LOCUS       NZ_CP079137             3692 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC04_AI1235MC
            plasmid pAI1235M_P6, complete sequence.
ACCESSION   NZ_CP079137
VERSION     NZ_CP079137.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20115068
            Assembly: GCF_019286515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC04_AI1235MC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC04_AI1235MC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:02:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,789
            CDSs (total)                      :: 5,665
            Genes (coding)                    :: 5,491
            CDSs (with protein)               :: 5,491
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 99 of 174
            Pseudo Genes (internal stop)      :: 26 of 174
            Pseudo Genes (multiple problems)  :: 25 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079137.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC04_AI1235MC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pAI1235M_P6"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            join(3429..3692,1..3)
                     /locus_tag="KWY69_RS28730"
                     /old_locus_tag="KWY69_28730"
     CDS             join(3429..3692,1..3)
                     /locus_tag="KWY69_RS28730"
                     /old_locus_tag="KWY69_28730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000262326.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRILCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(65..544)
                     /locus_tag="KWY69_RS28735"
                     /old_locus_tag="KWY69_28735"
     CDS             complement(65..544)
                     /locus_tag="KWY69_RS28735"
                     /old_locus_tag="KWY69_28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017896843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227525340.1"
                     /translation="MRKTLLLCVPLFLLAGCAQQKQQMPDANYEKFAKVEVASDACLKS
                     NFITAQEAGQAHSNISLFLSSWAYDYVRYSNLLAQGHEEVKKIKITQEGCNLLRAKIYQ
                     DNIEAQRYQKQMEMAAQQKALADQQALQSIQNMQNTLPKTTYCNQIGTQTICNSY"
                     /name="hypothetical protein"
     gene            complement(636..1067)
                     /locus_tag="KWY69_RS28740"
                     /old_locus_tag="KWY69_28740"
     CDS             complement(636..1067)
                     /locus_tag="KWY69_RS28740"
                     /old_locus_tag="KWY69_28740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_053274236.1"
                     /translation="MNIPTHLKHKPIIAVENYDRIDGPYADDTDAMGLSVGIAQWNTPG
                     YTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICIAMDYSKHGRLSSSDDFPVSRSG
                     GNPELSRHIDLMKNELKENHEYLDESLKRLAAELKRLGY"
                     /name="DUF6530 family protein"
     gene            2212..2439
                     /locus_tag="KWY69_RS28745"
                     /old_locus_tag="KWY69_28745"
     CDS             2212..2439
                     /locus_tag="KWY69_RS28745"
                     /old_locus_tag="KWY69_28745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224019247.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(2487..2675)
                     /locus_tag="KWY69_RS28750"
                     /old_locus_tag="KWY69_28750"
     CDS             complement(2487..2675)
                     /locus_tag="KWY69_RS28750"
                     /old_locus_tag="KWY69_28750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3221..3445
                     /locus_tag="KWY69_RS28755"
                     /old_locus_tag="KWY69_28755"
     CDS             3221..3445
                     /locus_tag="KWY69_RS28755"
                     /old_locus_tag="KWY69_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     Typing          complement(1354..1873)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.686"
                     /coverage="89.0"
                     /evalue="4.31e-144"
ORIGIN
        1 tgaagcccgc tatgcgggct ttttttgatg ttgacaacgg atgttatggt aaatcaattt
       61 atgatcagta gctgttacag attgtctgtg tgccaatctg gttacagtaa gttgttttag
      121 gtaaggtgtt ttgcatattt tgaattgatt gtaacgcctg ctgatctgct aaggcttttt
      181 gttgtgcagc catctccatc tgtttttgat atctctgtgc ctcgatgttg tcttgataga
      241 tttttgcgcg caaaaggttg cagccttcct gtgttatctt gatttttttt acttcttcat
      301 gtccttgcgc caagaggttt gagtaacgaa cgtagtcata cgcccatgaa cttaaaaata
      361 aggatatgtt ggaatgtgcc tgacctgctt cctgtgctgt aatgaagttg gattttaagc
      421 aggcatcaga ggcaacctcg acctttgcga acttttcgta attagcatct ggcatttgtt
      481 gtttttgttg ggcgcaacca gcaagtaaaa acaacggaac gcaaagtaat aacgtttttc
      541 tcatttagtg ttttatcctt ttttgacaag aaaaacctaa tgccccgtgg tgggtatata
      601 aaaaatcacg ggggagcgta gcgaccttcg tctacttaat aacctaaacg ttttagttct
      661 gctgctaaac gcttaagact ttcatctaaa tactcgtgat tctcttttaa ttcgtttttc
      721 atcaaatcaa tatgacgaga taactcagga ttacctcctg aacgactgac cggaaagtcg
      781 tcgctggaag ataagcgacc atgtttgctg taatccatcg ctatgcagat aagcgttgct
      841 aagtcgatga cacggtgaag aggtaactct tcagattgcc gtgaccactt ttctccggtg
      901 ttgcgccaga cttttgctga tagctcggta tatccggggg tgttccattg agcaattcca
      961 acggaaagcc ccatagcatc agtgtcatca gcataggggc catcaattcg atcatagttt
     1021 tctacagcga taatcggttt gtgtttcaga tgagtaggga tgttcatatc attctctgca
     1081 gtgtgaattt ggcttcttaa tcagtaaaca agaaaacaaa taaatttact aattagtaaa
     1141 tgtaacattt tatgaattgt tggatttgaa tttgttggag tggatcttct tgagatcatt
     1201 tatggctgag cgtaatctct tgctctgaaa acgaaaaaac cacctgggga ggtggttttt
     1261 cgaaggttag gtcagttggg gaactgctta accaggtaac tgtcttgtgc gagacgagtc
     1321 acctaaactg tccttccagt gtagccggac tcaggctccc acttcaagaa ctccagataa
     1381 atctctcgca catctcagtt accaatggct accgccactg gcgatttttc gtgtctgttc
     1441 cgggttggac tcaagtgata gttaccggga cgacgtggta gtcgggctga acggggggtt
     1501 cttgcataca gcccagcttg gagcgaactg tctaaacgga acggggcgtg gtgatttagg
     1561 taaagcttcc accacaacac ggacgccgca tgacgggaac aggagagcgc aagagggagc
     1621 catcaggtgg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg
     1681 agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggcttcgccg
     1741 tggccttctt gcttccctgt taagcttctt cctggaagat gccaggaaat ttccgccccg
     1801 ccagtaagcc ggtaccgctc gccgcagccg aacgaccgag cggagcgagt cagtgagcga
     1861 ggaagcggaa tatctccggg cttatctttg gcaccgtacg cgccatagct gcatttttta
     1921 atacgatgca ggatagggca gacgccgcaa aatgacgtca tccgacatca ttccgccgcg
     1981 ttcgcccagt gacctacagt cggctctggg gtgtcggggc tgtgccctga cacccgctaa
     2041 aagcactgac gcgcctgcgg cttgcccaac cccgcgccgt ccgggaaaga atttcccgat
     2101 cgacccgacg tttctgttta cctgtgatgt taacgtttgg atctttttcc cgggtttacc
     2161 tattttcttc taatgaagcg ttcacagctg acgttaatag atgggtgctt catgtttggt
     2221 ttttcaccag gagaacttat gaacctttgc cccgatgaac gtctgctctt tgtgcggatg
     2281 atttccgcta tgcttcgccg ttcgggcggt gatgcgggtg ctgttatgtt tgaggcgtat
     2341 cgccatattg tgtccgatac caatcaagct cgtcgttcct acatgctcga cctgctcgaa
     2401 agcgtcaggc atgactatgt acacggtgga tatacatagt tgccatggat acgcttttcg
     2461 tctaagtgag cgttattttc gtctctttat tcgccgtcct gtataccgaa gcgtgcgctc
     2521 agcttctgat aaagcgcttc tgcctgttcg tgcattcgct cacagtcggc tgcctcgtca
     2581 tcaagatcga gcacgtcgag tttttcgagc agcagcagcg actgcgcccg gataaatttt
     2641 gccatgttca gtgcggctga ttcctgtttt gtcatacggc tttttcccgg ttttatggtg
     2701 atcgcagact gaaatcttac ttcattcact tgagtgcata gccatgcagg tacgcagaaa
     2761 tcatcagggg cgttttttct cgatatgggc acagaaaggt gtatgtttca ggccggagtc
     2821 ctcagaaacg ccctgagggc gcctgaggtg gtgcgggcaa cagatgttat gttcaatcag
     2881 actgatctgc gggtacaaaa aaagccccgg agggctttta actactgact tatttttttg
     2941 aggatttgct gggtttcaat ttgtcagtat tccccggcgt ttttttattg tcacgctggc
     3001 gtttatcagg ttcccctgtt gaatcggcaa ttcgtttcgg acccggctta agacccataa
     3061 tcgttatcct ttcaatagtt aagaggaaag ccctcaagct ggatatattg acagcgttac
     3121 ggattatcaa gaggggccag caagattatt ttttgttctt ttaggttgat tgcgatacta
     3181 ttgctataca tgtatatcaa atggtgttca ggaggtttat atgcttgcta tccggttatc
     3241 tgatgagatt gagtcccgtc tggactcgct ggcgaagcaa accggcagaa caaagacgtt
     3301 ctatgcgcgg gaagcaatac tggcgcatct ggaagacctg gaagattatt atctttcagc
     3361 agaaactgcc gcacgcgttc gccgtggtga tgaagccgtg cattcgtctg aagacgtgag
     3421 gaagtcactt ggtctggacg attaactatt ccgaccgggc gctcaaatcg ttacgcaaga
     3481 tggacaaaca gaacgcacga cggattgtgg attttatgga tttacgcatt gcagttgctg
     3541 ccgatcctcg ccagtcaggg aagccgctca aaggtgagct gggcgagttc tggcgctatc
     3601 gggtgggaga ttatcgcatt ctgtgtgaga tccgggatga cgagctggtt atccttgccg
     3661 ccaccattgg ccatcgccgc gaagtttacg ac
//
