LOCUS       NZ_CP079136             9117 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC04_AI1235MC
            plasmid pAI1235M_P5, complete sequence.
ACCESSION   NZ_CP079136
VERSION     NZ_CP079136.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20115068
            Assembly: GCF_019286515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC04_AI1235MC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9117)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC04_AI1235MC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:02:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,789
            CDSs (total)                      :: 5,665
            Genes (coding)                    :: 5,491
            CDSs (with protein)               :: 5,491
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 76 of 174
            Pseudo Genes (incomplete)         :: 99 of 174
            Pseudo Genes (internal stop)      :: 26 of 174
            Pseudo Genes (multiple problems)  :: 25 of 174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079136.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9117
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC04_AI1235MC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pAI1235M_P5"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            1..948
                     /locus_tag="KWY69_RS28670"
                     /old_locus_tag="KWY69_28670"
     CDS             1..948
                     /locus_tag="KWY69_RS28670"
                     /old_locus_tag="KWY69_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            1356..1748
                     /locus_tag="KWY69_RS28675"
                     /old_locus_tag="KWY69_28675"
     CDS             1356..1748
                     /locus_tag="KWY69_RS28675"
                     /old_locus_tag="KWY69_28675"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            1730..2068
                     /locus_tag="KWY69_RS28680"
                     /old_locus_tag="KWY69_28680"
     CDS             1730..2068
                     /locus_tag="KWY69_RS28680"
                     /old_locus_tag="KWY69_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2049..3503
                     /locus_tag="KWY69_RS29040"
     CDS             2049..3503
                     /locus_tag="KWY69_RS29040"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3785..4066)
                     /locus_tag="KWY69_RS28700"
                     /old_locus_tag="KWY69_28700"
     CDS             complement(3785..4066)
                     /locus_tag="KWY69_RS28700"
                     /old_locus_tag="KWY69_28700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            4410..5207
                     /locus_tag="KWY69_RS28705"
                     /old_locus_tag="KWY69_28705"
     CDS             4410..5207
                     /locus_tag="KWY69_RS28705"
                     /old_locus_tag="KWY69_28705"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            <5438..5797
                     /locus_tag="KWY69_RS28710"
                     /old_locus_tag="KWY69_28710"
                     /pseudo=""
     CDS             <5438..5797
                     /locus_tag="KWY69_RS28710"
                     /old_locus_tag="KWY69_28710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016943399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /name="WbuC family cupin fold metalloprotein"
     gene            complement(5844..6719)
                     /locus_tag="KWY69_RS28715"
                     /old_locus_tag="KWY69_28715"
     CDS             complement(5844..6719)
                     /locus_tag="KWY69_RS28715"
                     /old_locus_tag="KWY69_28715"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-15"
                     /protein_id="WP_000239590.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQSG
                     GRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKKSD
                     LVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGDETF
                     RLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNTTGAA
                     SIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESRRDVLA
                     SAAKIVTDGL"
                     /name="extended-spectrum class A beta-lactamase CTX-M-15"
     gene            complement(6975..8237)
                     /locus_tag="KWY69_RS28720"
                     /old_locus_tag="KWY69_28720"
     CDS             complement(6975..8237)
                     /locus_tag="KWY69_RS28720"
                     /old_locus_tag="KWY69_28720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEcp1 family transposase"
                     /protein_id="WP_000608644.1"
                     /translation="MINKIDFKAKNLTSNAGLFLLLENAKSNGIFDFIENDLVFDNDST
                     NKIKMNHIKTMLCGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFNFKTT
                     QMFRDINFKVFKKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKKLGNRCYNIQF
                     AFCDELKAYVTGFVRSGNTYTANGAAEMIKEIVANIKSDDLEILFRMDSGYFDEKIIET
                     IESLGCKYLIKAKSYSTLTSQATNSSIVFVKGEEGRETTELYTKLVKWEKDRRFVVSRV
                     LKPEKERAQLSLLEGSEYDYFFFVTNTTLLSEKVVIYYEKRGNAENYIKEAKYDMAVGH
                     LLLKSFWANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAAKIIKTAR
                     YVIMKLSENYPYKGVYEKCLV"
                     /name="IS1380-like element ISEcp1 family transposase"
     gene            complement(8590..>8742)
                     /locus_tag="KWY69_RS28725"
                     /old_locus_tag="KWY69_28725"
                     /pseudo=""
     CDS             complement(8590..>8742)
                     /locus_tag="KWY69_RS28725"
                     /old_locus_tag="KWY69_28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     ARG             complement(5848..6719)
                     /name="blaCTX-M-15"
                     /gene="blaCTX-M-15"
                     /gene_name="extended-spectrum class A beta-lactamase
                     CTX-M-15"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4411..5204
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          14..935
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          2050..3500
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgagaaagt gcgcacagct tgagctgttt cgggagcgcc tgccccggaa accctactat
       61 tccgacgagc ttacaacggg cctacggatt gcggatgttg cccgcgccct tggtgcgcgt
      121 tacatccagc cgaacgggcc aacgcaccgc cactggatcg tttttgacgt agaccatgca
      181 gccgctacgt tatcttggga tgacgtaggc gctccagccc ctaacattac cgtcacgaac
      241 aaggccaacg gccacgcgca tctgatctac ggactggata cgccgatccg cactgccccg
      301 gatggaaatg cagcccctct gcggtatgcc gccgccatcg aggccgcgct ccgggagaaa
      361 ttaggggcgg atatgggcta ctccgggctg atctgcaaga accccctgca tgagcactgg
      421 ctggtgcagg tatgggaacc ccggctctac gatttagcct ggctatccga ttacctcgat
      481 ctgagctcct acaatggccg caaatcgctt cctgagtacg ggcttgggcg aaactgtacc
      541 ctgttcgaga aaacgcgcct gtgggcctat aaagcgatcc ggcagggctg gccggattac
      601 cccgaatggt tggccgcttg tgttgaccgg gcatcaggat ataacgccca gttcgagcag
      661 ccgctccccg caaacgaagt ccggcacacg gccaagtcca ttgccaaatg gacgcaccag
      721 cacctaagcc ccgcagggtt cagagaggag caggcccgga gaggtgcgaa gggcggcaag
      781 gtatccaagg gagggggaag gccgagcaat agcggtaagg acaagtccga tctattgccg
      841 gaggttctgc ggcttaaggc tcaggggtac accaaccgcg atatagcgga cgatcttcaa
      901 atctccccca gtacggtgag cgtttaccta aagcgtgatc gtccgtgatc gaaacaagcc
      961 ttatcagata atagccccct ttaggggggt tcctgcgctc ttgtctttta gccttaaaaa
     1021 atccttttag cgcggatctt tggcgaggtg gacaactgcg cctgtccgtc catcgccccc
     1081 ctgttttttc ggggggtgtg ggcggcagca gttatccacg gagccgctct ttcccttgat
     1141 cttgctctag ggcttgcagt atcccgccca tggttgaata atgtactaag caaggctaca
     1201 cagaggcccc tctacctgct tcgcggtctc gggcctctgt ggccttgttc aggggtgcta
     1261 cgcccccccg aaaccccctg tggcccccgc tcgatccgcc ccttcgggac ggtctgcgcg
     1321 gtggcttctt ggctctggag aatggacgag gccctatgtc aacaacacag atagagaaag
     1381 attcgagaag gaaggcagtc aatggcgtta tttctagttt ccaactagag ggggaggcca
     1441 taaccgatga attcaaaatt ctacttgaag attatatcaa tggaaaaatt tccaccgatg
     1501 aggtgttagc tatggtaagt gacgaaccca caacaacgga atacgactac gaaatcaccc
     1561 cccgcactct cggtcttggc gggggctgga atctgcgttt gctggagaac ggcgaagagg
     1621 tcggcggggg tgttttcccc ttgccggagc actgcgattt tcgggatgaa aaggcgctcc
     1681 agacgctact cgatagcctc tatgaggacg ccttagcgga ggcctctgca tggctggcca
     1741 gccgttaaag cgcctccgct ctatcaagat ccgggtctca gatgcggagc ttgagcgcct
     1801 gcgggagctc tgccccaagg cccaactggc ggagtggatg cgggagcaat gtctcggtgt
     1861 tgcgcaaccc cagcgccgaa cgccggcgcc taccgtagat ccggcgctct tgcggcagtt
     1921 ggccggaatg ggcaacaacc tgaaccagat cgcccggcgc gttaattccg gggagtgggg
     1981 gccgttggat cggctgcgca tcatagcgga gctgtccgct ataggccgcg agctggagga
     2041 actgcaccat gatcatcaaa ttccataacc gtggggtagg ccggggctcc gggcctgttg
     2101 gctacttgtt gggccgtgac gggaaccggg aaggcgcaac gctcgaccgg ggcaaccctg
     2161 cggtgattga agccctgatt gattccagtc cctacgccaa aaaatacacc tctggcgttc
     2221 tgtcgtttgc cgaggctgat ttacccaggg cgaccaagga caagctgatg agcgactttg
     2281 aaaaggcgct cctgcccgga ctggagggca accagtacgc tgttttgtgg gtagagcacc
     2341 gcgacaaggg gcggctggag ctgaattttg tagtcccgaa catcgaattg cagagcggga
     2401 agcggctgca accctacttc cacaaggccg acaatccgcg cctcgatgcg tggcgaaccg
     2461 tggtaaacgg ggagctgaaa ttgcacgacc cggacgatcc gttaaaccgt caggccgtta
     2521 tcacgccaaa ggatttgccg agggggcaca aggaggccgc tcaggccatt acagacgggc
     2581 ttttacgcat ggccggagca ggggagatcc agagccgcga tgatgtgcgc aaagccttgc
     2641 agggtgcggg tttcagcgtg gtacgcgaaa ccaaaaacag cctgagcatt gccgacccgg
     2701 acggggggcg gaacatccga ttaaaggggg tgctgtatga gcaagatttt agacatggcg
     2761 aaggacttcg agcagagctc gaagcagcag gccaacgcta tcgagaagcg tctgaccgaa
     2821 gaattcacga ggcacgagaa agctatctcc ggggcgttga aatcaagcga ggagaaaatg
     2881 agcgccgcta ttgccgagca cagccagaaa atgggctggc tgttgctcaa aacgtggatc
     2941 tggatgctgg tcagcatcgt cctgttcttg agcgcaacga gcgggatcct ctggtatcag
     3001 ggggaattga tagcggagcg gcaagccact ctccaagcct tggcagagaa gggcggcaag
     3061 ctaaagctga cccagtgcgg gaagaaattg gagcaccttt gcgtgcagat ctccgacccc
     3121 aatccaaccg tttgggggaa ggatacctat tggatcgtgc cgaaggggta ctaaatgacg
     3181 ggattggagc gggaattgct ctgcgcgttc gaggctttgc agagcgcata cgagcagcag
     3241 cacaaggcat ggcaggaaag ctacgcgaac ttggcgagca tgtacgagag caccaaacaa
     3301 gaggccgaga tcagcagggc agcagcagag cgattgacga ggcaggtgaa cggcttaacg
     3361 cagcaagtgg acggcttgag caagcagctg gagggcttga ccgggctagt cagcaagttg
     3421 caaaagaaag ggaacacagg ctcctgaagg cactggatcg aggctgggag cgtggagaca
     3481 gatcgcgagg ctttgagcgt tgacggaaca aaacggccgt cagttataaa aaaaggaaag
     3541 tgctatcact ttccttttat cggcgggaat tatcgttgag ttcttctatg ccatttttca
     3601 tagtagtaac tgtaatcttc atctctacca ccagattcaa tccagtcatc atactcaggg
     3661 ctatttttga aatctgaatc agacatgcta actccttgtt attagttggc ttctatggaa
     3721 gctagcgcac cgtgaagtta tgatcaacga tgtagtccac atcattgtcg aaatgtgcgt
     3781 tcagctagtc ttcggtgttt tctgctttat ttctagcggc gattctcgcg tctgcccatg
     3841 cggcagactt tttagcctgt tctcgttgtt cttcggttga agactcgctg ttgagaactt
     3901 tgttgctcgt ttgtttgacc accgaatcca aacctgttgt tgctgcaaaa tgagctaggt
     3961 taacaactcc cttacctaac aacttggcac tagcgattgc ggtttcttta gccccaaaag
     4021 catcagacac ctcctcaagc gctttagata ggttaaaaaa tcccatactc cccctaagaa
     4081 tatcatcaat aaaattgagt gttgctctgt ggataacttg cagagtttat taagtatcat
     4141 tgcagcaaag atgaaatcaa tgatttatca aaaatgattg aaaggtggtt gtaaataatg
     4201 ttacaatgtg tgagaagcag tctaaattct tcgtgaaata gtgatttttg aagctaataa
     4261 aaaacacacg tggaatttag gtaatagtta aagcttgtgc ctgattgttg ccacaattgg
     4321 tcagcagttt gctagggaat gagagggcag agaatgataa gcttagcact taatttgtat
     4381 ggatatattg cattaagcaa ggggacgtta tgcgtgtatt agccttatcg gctgtgtttt
     4441 tggtggcatc gattatcgga atgccagcgg tagcaaagga atggcaagaa aacaaaagtt
     4501 ggaatgctca ctttactgaa cataaatcac agggcgtagt tgtgctctgg aatgagaata
     4561 agcagcaagg atttaccaat aatcttaaac gggcgaacca agcattttta cccgcatcta
     4621 cctttaaaat tcccaatagc ttgatcgccc tcgatttggg cgtggttaag gatgaacacc
     4681 aagtctttaa gtgggatgga cagacgcgtg atatcgccgc ttggaatcgt gaccatgact
     4741 taattaccgc gatgaagtac tcagttgtgc ctgtttatca agaatttgcc cgccaaattg
     4801 gtgaggcacg tatgagtaaa atgctgcacg ccttcgatta tggcaatgag gatatctcgg
     4861 gcaatgtaga cagtttttgg ctcgatggtg gtattcgcat ttcggctacc cagcaaatcg
     4921 cttttttacg caagctgtat cacaacaagc tgcacgtttc tgagcgtagt cagcgcatcg
     4981 tgaaacaagc catgctgacc gaagccaatg gcgactatat tattcgggct aaaacgggat
     5041 actcgactag tatcgaacct aagattggct ggtgggttgg ttgggttgaa cttgatgata
     5101 atgtgtggtt ttttgcgatg aatatggata tgcccacatc ggatggttta gggctgcgcc
     5161 aagccatcac aaaagaagtg ctcaaacagg agaaaattat tccctagaag tggttagcgc
     5221 gtatttgtgt gaaatagccg tcatataagc tgtaaagata tatggacaaa atgctgatgc
     5281 tcgatgcgct tgtagttaat ggctcatact taatcggtca ggcctaatcg acttaagctc
     5341 cgcgcgttaa ctttcagcct gagcattagc atcacattcc taaacgtaaa aagcctagcc
     5401 aatttgctag gcttttttgt tatctcaatg gccatggtca ctcaacatgg ggcctgggac
     5461 ctacgtgcgc ccgcaccgac accctcacac cttcgagcta ctgttgccat taaggggtcg
     5521 tttcgtggtg ctgaattttg acgatcgggg taccgtcacc catcgggcga tattggggga
     5581 aacctgtacg gtgctggaga tggccgcagg aacctggcat gccgtgctgt cgctggatac
     5641 cggtggcata atttttgaag taaaacacgg tggctatcaa cccgtggctg ccgatgacta
     5701 tgcgcactgg gctccagcgg aaggagaacc aggaaccacg gagcttatgg cctggtatgc
     5761 gcaagcgcag gtgggcgaca gcacttttgc cgtctaaggc gataaacaaa aacggaatga
     5821 gtttccccat tccgtttccg ctattacaaa ccgtcggtga cgattttagc cgccgacgct
     5881 aatacatcgc gacggctttc tgccttaggt tgaggctggg tgaagtaagt gaccagaatc
     5941 agcggcgcac gatcttttgg ccagatcacc gcgatatcgt tggtggtgcc atagccaccg
     6001 ctgccggttt tatcccccac aacccaggaa gcaggcagtc cagcctgaat gctcgctgca
     6061 ccggtggtat tgcctttcat ccatgtcacc agctgcgccc gttggctgtc gcccaatgct
     6121 ttacccagcg tcagattccg cagagtttgc gccattgccc gaggtgaagt ggtatcacgc
     6181 ggatcgcccg gaatggcggt gtttaacgtc ggctcggtac ggtcgagacg gaacgtttcg
     6241 tctcccagct gtcgggcgaa cgcggtgacg ctagccgggc cgccaacgtg agcaatcagc
     6301 ttattcatcg ccacgttatc gctgtactgt agcgcggccg cgctaagctc agccagtgac
     6361 atcgtcccat tgacgtgctt ttccgcaatc ggattatagt taacaaggtc agattttttg
     6421 atctcaactc gctgatttaa cagattcggt tcgctttcac ttttcttcag caccgcggcc
     6481 gcggccatca ctttactggt gctgcacatc gcaaagcgct catcagcacg ataaagtatt
     6541 tgcgaattat ctgctgtgtt aatcaatgcc acacccagtc tgcctcccga ctgccgctct
     6601 aattcggcaa gtttttgctg tacgtccgcc gtttgcgcat acagcggcac acttcctaac
     6661 aacagcgtga cggttgccgt cgccatcagc gtgaactggc gcagtgattt tttaaccatg
     6721 ggattcctta ttctggaaga tacgaaataa caacaacatg aatagtccct aaattccacg
     6781 tgtgtttttt attagcttca aaaatcacta tttcacgaag aatttagact gcttctcaca
     6841 cattgtaaca ttatttacaa ccacctttca atcatttttg ataaatcatt gatttcatct
     6901 ttgctgcaat gatacttaat aaactctgca agttatccac agagcaacac tcaattttat
     6961 tgatgatatt cttattatac cagacatttt tcatacactc ccttgtacgg atagttttcc
     7021 gacaacttca tgattacata tcttgcggtt ttgattattt ttgctgcaag aaatacatac
     7081 ttcaaacgaa aggtctttat ttgctgtctg tattctgaag agtccaagga atcaaacttg
     7141 aacaacaaaa ataggttata tgaaagcatc atcatttgaa acacggcttc attcgcccaa
     7201 aatgacttta gcaagagatg acccaccgcc atgtcgtatt tggcttcttt gatatagttt
     7261 tcagcattac cacgcttttc atagtatata actacttttt cagaaagcaa ggtagtattt
     7321 gttacaaaga aaaagtagtc gtattcggaa ccttctaaaa gtgataattg tgctctttct
     7381 ttttctggtt tcagtacgcg agatacgaca aatcttctgt ctttttccca tttaactaat
     7441 tttgtataca gttctgtagt ttctctacct tcttctcctt taacgaatac aattgatgaa
     7501 ttcgttgctt gtgaggtgag tgtagaataa cttttggctt taattaaata tttgcatcca
     7561 agagattcta tcgtttcgat aattttttca tcaaagtagc cactatccat tcgaaataaa
     7621 atttctaaat cgtctgattt gatgttagca acaatttctt tgatcatttc cgcagcaccg
     7681 tttgcagtgt aagtattgcc acttcttaca aatccggtaa catatgcttt taattcgtcg
     7741 caaaatgcaa attggatatt gtagcatcgg tttcccagtt tcttaggatt atatcctttt
     7801 gacgcacctt cttgatgacc ttctacgtta attacactac tatcaatatc aatcgtaatg
     7861 gatgtcaatt tacttttagt gagcagtttt ttaaagactt taaaattaat gtctctaaac
     7921 atttgggttg tcttgaagtt gaagtttcct agaaaccgtg acactgtttc aggttctttt
     7981 acggaaatat caaactcgtt gacgagggga tcattttgaa gtagctttag acgttctaac
     8041 ttatcaatgc caatgaagtg accgcagagc atggtcttta tatgattcat cttgatttta
     8101 tttgttgagt cattatcaaa tacgaggtca ttttcaataa aatcaaaaat cccattgctt
     8161 tttgcattct caaggagcag aaaaagacct gcatttgatg ttagattctt agctttgaaa
     8221 tcaattttat taatcataat tagaacccct ttttactact tttcttacta ttattttacc
     8281 atatatcgag tcataaaagc tgataattta acatattttt gagcactttt ctttcaccca
     8341 atgggtgaaa gctgaatttc gaaggaatgc atatttatca aggctttgat tatgcttttt
     8401 gaagtactga cgtagaatct aggtgggggt gtgaataaag gagtggcgat gtcagtaaag
     8461 gtgaatgccg ctgcgataaa gtgcctagcc catcaaggat tgtaggcttt cttggctgct
     8521 aaagcgtttg acgaaaaagt ccaagaaggc gctgatattg gttctccatc attttcatct
     8581 accggtttac tataggtcaa ccaggccgtc cttgccggcg cttgggacgc agacgatggc
     8641 atcaaatgcc tcgctcaaat tcgtttctac agaggcgctt tgggaccgca ttcgctttgc
     8701 tttcatgggg gcatctgtca atgtcagaca tgaatcctcc gatataggtt gatttttgat
     8761 ctcttcaaca cttggtgttg tccgactaga gagacttata tggatcagta ttcttgcact
     8821 gacaatggtg ccactcaatc ccaaggatcg acctagttag aggcgtagat caagcaattc
     8881 aaaacagcgc agaagcagaa ggaggtggaa atatgttcca gggtgaaagg ttacatgcaa
     8941 ctaacgttgc tctctaggat gtgcctgctg taacgtattg tccagaaaag acaaaccccc
     9001 gttatcgctt gcatcttggc ggagctgcga aaaacggggg ccgtgacgaa tcacaagaga
     9061 agtctatcac atggatcatg ccaaacaacc ccacgccgcc aataagcgcg gtggtgc
//
