LOCUS       NZ_CP079127            10654 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC03_AI1235PC
            plasmid pAI1235P_P7, complete sequence.
ACCESSION   NZ_CP079127
VERSION     NZ_CP079127.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20115067
            Assembly: GCF_019286455.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10654)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC03_AI1235PC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10654)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC03_AI1235PC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:15:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,786
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,489
            CDSs (with protein)               :: 5,489
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 74 of 173
            Pseudo Genes (incomplete)         :: 99 of 173
            Pseudo Genes (internal stop)      :: 26 of 173
            Pseudo Genes (multiple problems)  :: 24 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079127.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10654
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC03_AI1235PC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pAI1235P_P7"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            join(<10654..<10654,1..221)
                     /gene="fhuF"
                     /locus_tag="KWV87_RS28595"
                     /old_locus_tag="KWV87_28595"
                     /pseudo=""
     CDS             join(<10654..<10654,1..221)
                     /gene="fhuF"
                     /locus_tag="KWV87_RS28595"
                     /old_locus_tag="KWV87_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224976.1"
                     /GO_function="GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]; GO:0008199 - ferric iron binding [Evidence
                     IEA]; GO:0016491 - oxidoreductase activity [Evidence IEA]"
                     /GO_process="GO:0033214 - siderophore-dependent iron import
                     into cell [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /name="fhuF"
     gene            complement(271..1614)
                     /locus_tag="KWV87_RS28600"
                     /old_locus_tag="KWV87_28600"
     CDS             complement(271..1614)
                     /locus_tag="KWV87_RS28600"
                     /old_locus_tag="KWV87_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="WP_002887650.1"
                     /translation="MSANHAAFNLIFRFVENYISPIAGRISSQRHVMAIRDGFISAMPF
                     MIVGSFLLVFAYPPFSPDTTWGFARAWLDLAKEFEGRILTPFDMTMGIMSIYICAAISY
                     NLGKHYEKSNQLDPFMCAMLSIMAFLLIAAPKTNGTLPVDSLGGTGIFTAILVAIYCVE
                     MMRFLKAHNIGIRLPDQVPPMIKNSFDLLIPVLVVVLTLYPLSLFIQHHFDMLIPQAIM
                     AIFKPLVSAADSLPAILLAVLIGHLLWFAGIHGAAIVSGMLQMFWLTNLGMNQQALAQG
                     APLPHIFMEAFWTFFIVVGGSGATMGLVFCYLRSRSAHLRSIGRLSVVPSLFNINEPVI
                     FGTPIVMNPVFFIPFLLAPMVNAVLAWAAMKLDLIGRVISVVPWTAPAPIGGAWALGWD
                     FRAAILVIVLACVSAIIYFPFFKVYEKQLLAQEAEEAERAEQESQQTA"
                     /name="PTS cellobiose transporter subunit IIC"
     gene            1952..2410
                     /locus_tag="KWV87_RS28605"
                     /old_locus_tag="KWV87_28605"
     CDS             1952..2410
                     /locus_tag="KWV87_RS28605"
                     /old_locus_tag="KWV87_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="WP_004151383.1"
                     /translation="MSLQSVRQFFAEHAPDIEIIELNQSTATVALAAAAHNVEPGQIAK
                     TLSLKIKDKIVLIVARGDARLDNKKLKETFGAKARMLSSDEVVTWTGHPVGGVCPFGLE
                     NPLAVYCDVSLRHYQEVLPAAGAIHSAVRIEPERMAQLTDATWVDVCL"
                     /name="YbaK/EbsC family protein"
     gene            complement(2416..3090)
                     /gene="bglJ"
                     /locus_tag="KWV87_RS28610"
                     /old_locus_tag="KWV87_28610"
     CDS             complement(2416..3090)
                     /gene="bglJ"
                     /locus_tag="KWV87_RS28610"
                     /old_locus_tag="KWV87_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224973.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="WP_002887647.1"
                     /translation="MEKNSTTRHVAIVEKCVMTEMAWRYIFSREDNRRYHIHLFKNVSM
                     LRAAEIPFPWSTIIFSLSGSRSSRAESLLFVHEMARCRPEVQTIILANNESEMHLIGHL
                     MPACLHGILNKSAPRRGLQERLLQLLDNHHLSGSEQFCDREACNDPLSPTEHAILRYMS
                     WGYSLSDIAIQLNRNIKTIRAHKFNAMTKLGVRSDIGLLNAADILLHLPLNSTTSAVKQ
                     VA"
                     /name="bglJ"
     gene            complement(3051..3773)
                     /locus_tag="KWV87_RS28615"
                     /old_locus_tag="KWV87_28615"
     CDS             complement(3051..3773)
                     /locus_tag="KWV87_RS28615"
                     /old_locus_tag="KWV87_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224972.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="WP_002887645.1"
                     /translation="MLPGQGKFGVVISKIPVMQSGLKVIFNENLPDYELSFCRSHDELT
                     LLQLRRAVLVVADISGEIAHPRAVCERYYSLMTQYRDIHWVFMVSDSLYPLAVELLIRP
                     ESSLISESEPVNRLIEVICAGSRAAEQISRTLFAGDEDSDFASYHSAAMLTLSERKVLR
                     LLAKGWGINQIAALLKKSNKTISAQKNSAMRRLSLRSNAEMYAWINSAQGMQELNINSA
                     YGEQMEWKKIVQPAMSPS"
                     /name="LuxR C-terminal-related transcriptional regulator"
     gene            complement(4039..4920)
                     /locus_tag="KWV87_RS28620"
                     /old_locus_tag="KWV87_28620"
     CDS             complement(4039..4920)
                     /locus_tag="KWV87_RS28620"
                     /old_locus_tag="KWV87_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224971.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]; GO:0043565 - sequence-specific DNA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002887643.1"
                     /translation="MPFVQTVPVFKLYGEECGWPTPDLLHCESILQRSSLYQWHIRVHQ
                     HAEMVQLLYLHQGRAEIEIEGATAVMTESCIQVVPALCIHGFNFSPGTQGFVLSLALPL
                     LSRFENQFGRPLDVLSVPQCVPVGRSRGRMHTLFSALREEYCEEHDAREMMLYSLLGTL
                     LVWLNRQCHPASPAEDKAERKRSMMRHFARLIESHYRQHLPLAEYAKLIGLSVTHLNYL
                     CREFYGCSALGVLHQRLMLEARRNLQYTRMTITQLSDYLGFSDAAYFSRFFRRYSGMSP
                     KAFRETIKDNAS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4937..6121)
                     /gene="pobA"
                     /locus_tag="KWV87_RS28625"
                     /old_locus_tag="KWV87_28625"
     CDS             complement(4937..6121)
                     /gene="pobA"
                     /locus_tag="KWV87_RS28625"
                     /old_locus_tag="KWV87_28625"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224970.1"
                     /GO_function="GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA]"
                     /GO_process="GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="WP_004178539.1"
                     /translation="MKTQVAIIGAGPSGLLLGQLLHNAGIHTVILERQTPQYVLGRIRA
                     GILESGTVDLLREAGVAQRMDAEGLVHHGVEFLFDGQRVPVALSELTDGKSVMVYGQTE
                     VTRDLMAARAASGAPIVYGVSEVAIHDAKSDRPTITYLSEGETCRLECDFIAGCDGFHG
                     VSRQSIPAGILQTYESVWPFGWLGLLADTPPVNPELIYAHHQRGFVLCSQRSLTRSRYY
                     LQVPLSDKVEAWSDERFWQELKSRLPEELASRLVTGHSLEKSIAPLRSFVVEPMQYGRL
                     FLVGDAAHIVPPTGAKGLNLAASDVNYLWRILREYYHRGRSDLLATYSQLALDRVWKGE
                     RFSWFMTRLLHDFPDQNAFDAKMQAADRRYYLGSRAGLTTIAENYVGLPMERVA"
                     /name="pobA"
     gene            6399..7175
                     /locus_tag="KWV87_RS28630"
                     /old_locus_tag="KWV87_28630"
     CDS             6399..7175
                     /locus_tag="KWV87_RS28630"
                     /old_locus_tag="KWV87_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224968.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="WP_004146059.1"
                     /translation="MQVDRTQQRAITRLCIQCGLFLLQHGAESALVEELSTRLGLALGM
                     DSVESAISSNAIVLTTIKNGECLTSTRKNVDRGINMHVVTEVQHIVILAEHKLLDYRDV
                     EKRFAQIVPLRYPRWLLVLMVGLSCACFCKLNNGGWDGALVSFCASTVAMYIRQVLTHR
                     SMHPQINFCITAFVATTISGLLLRLPAFASTPTIAMAASVLLLVPGFPLINAVADMFKG
                     HINTGLARWAIASLLTLATCIGVVMAMTVWGLRGWA"
                     /name="threonine/serine exporter ThrE family protein"
     gene            7166..7639
                     /locus_tag="KWV87_RS28635"
                     /old_locus_tag="KWV87_28635"
     CDS             7166..7639
                     /locus_tag="KWV87_RS28635"
                     /old_locus_tag="KWV87_28635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="WP_002887631.1"
                     /translation="MGIISFIFALAEDMLLAAIPAVGFAMVFNVPQRALRWCALLGAIG
                     HGSRMIMMSAGFNIEWATFLAALLVGSIGIQWSRWYLAHPKIFTVAAVIPMFPGISAYT
                     AMISAVKISHFGYSEEMMILLLSNFLKASSIVGALSIGLSIPGLWLYRKRPRV"
                     /name="threonine/serine exporter"
     gene            7742..8281
                     /gene="dnaT"
                     /locus_tag="KWV87_RS28640"
                     /old_locus_tag="KWV87_28640"
     CDS             7742..8281
                     /gene="dnaT"
                     /locus_tag="KWV87_RS28640"
                     /old_locus_tag="KWV87_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="WP_004178536.1"
                     /translation="MSSRILTSHFSGLEEFLQQHAALLAKSTDGTVAVFANNAPAFYAL
                     TPARLAQLLELEARLARPGSDIALDPQFFEEPAAAPVAVPMGKFAMYAGWRPDADFQRL
                     AALWGIALSQPVTPEELAAFVAYWQAEGKVFHHVQWQQKLARSVQISRASNGGQPKRDV
                     NSVSEPDSHIPRGFRG"
                     /name="dnaT"
     gene            8284..9021
                     /gene="dnaC"
                     /locus_tag="KWV87_RS28645"
                     /old_locus_tag="KWV87_28645"
     CDS             8284..9021
                     /gene="dnaC"
                     /locus_tag="KWV87_RS28645"
                     /old_locus_tag="KWV87_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="WP_002887623.1"
                     /translation="MKNVGDLMKRLQKMMPAHIEPAFKTGEELLAWQKEQGRLRSEALE
                     RENRAMKMQRTFNRSGIRPLHQNCSFDNYRVECEGQMNALARARQYVEEFDGNIASFIF
                     SGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFGNRETSEEQLLNDLSMV
                     DLLVIDEIGMQTESRYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMNKLLGERVMDRMR
                     LGNSLWVIFNWESYRHRVTGKEY"
                     /name="dnaC"
     gene            9087..9578
                     /locus_tag="KWV87_RS28650"
                     /old_locus_tag="KWV87_28650"
     CDS             9087..9578
                     /locus_tag="KWV87_RS28650"
                     /old_locus_tag="KWV87_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="WP_002887620.1"
                     /translation="MKSFKNSVIAALAVSAFTLSGAHAASWQDSLNSAASQLSGSNAQA
                     SGGMSVSSLTSLLNGSSQSLTASSMNNAAGILSYCAKQKLASVTSADNVKNQVLDKLGL
                     STPEKQKQDTSYLDGLQGLLNSKNGQQLDLNTLGNSSLAKQVKTKACDLVLKQGVNFLS
                     "
                     /name="DUF2501 domain-containing protein"
     gene            9836..>10654
                     /locus_tag="KWV87_RS28655"
                     /old_locus_tag="KWV87_28655"
                     /pseudo=""
     CDS             9836..>10654
                     /locus_tag="KWV87_RS28655"
                     /old_locus_tag="KWV87_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224963.1"
                     /note="catalyzes the transfer of phosphoglycerol to the
                     glucan backbone; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerol transferase I"
                     /name="phosphoglycerol transferase I"
ORIGIN
        1 tgattaactg gtatcttggg gaaatgcgcg cgctgctggg tgatgagcgg cttgccgcgc
       61 tacgtcagca ctgttttttt gaaaaacaac tcgccgatgg ccaggataac ccgctgtggc
      121 gaacggtgat gctgcgtgaa gggcagttag tccgacgcac ctgctgtcag cgctatcgcc
      181 tgccggatgt ccaacagtgc ggcgactgta cgcttaaata attaccactt acctgaaaag
      241 taacgggcgg cctttaccgc ccgttgctat ttatgcggtc tgttgactct cctgctccgc
      301 ccgctcggcc tcttccgctt cctgagcaag cagctgtttc tcatatacct taaagaatgg
      361 gaagtagatg attgccgaca cgcaggccag aacaatgacc agaatcgccg cccggaaatc
      421 ccagcccagc gcccaggcgc cgccaattgg cgctggcgcc gtccatggta ctacggagat
      481 gacgcggcca atcagatcca gcttcattgc cgcccaggcc agcaccgcgt tcaccatcgg
      541 cgccagcagg aacggaataa agaacactgg gttcatgacg ataggcgtcc caaagatcac
      601 cggctcgttg atattgaaca ggcttggcac cacgctcaaa cgaccaatgg agcgcaaatg
      661 cgctgaacgg ctgcgcagat aacagaacac cagtcccatg gtcgccccgg aacctccgac
      721 cacgataaag aaggtccaga acgcttccat aaagatatgc ggcagaggcg cgccctgggc
      781 cagcgcctgc tggttcatac cgaggttagt cagccagaac atctgcagca tcccggacac
      841 gatcgccgca ccgtggatcc cggcaaacca cagcaggtgg ccgatcagca ccgccaacag
      901 aattgccggc agagagtcgg ctgccgaaac cagcggcttg aaaatggcca taatggcctg
      961 cgggatcagc atgtcaaaat ggtgctggat aaacaggctc agcggataga gcgtcagcac
     1021 gaccaccagc accgggatca acagatcaaa cgagtttttg atcatcggcg gaacctggtc
     1081 cggcaagcgg atcccaatgt tgtgcgcttt caagaaacgc atcatctcca cgcagtagat
     1141 cgccaccagg atggcggtaa agatcccggt ccccccgagg ctatccaccg gcagcgtgcc
     1201 attggtcttc ggcgcggcaa tcagcaaaaa ggccatgatc gacagcattg cgcacataaa
     1261 gggatcgagc tgattggatt tctcgtaatg cttaccgagg ttataggaga ttgccgcgca
     1321 aatgtagatc gacatgatcc ccatcgtcat atcaaagggg gtcagaatac gcccctcaaa
     1381 ctctttcgcc agatccagcc aggcgcgggc aaaaccccag gtggtatccg gtgagaacgg
     1441 tggataagca aacactaaca ggaacgatcc gacgatcata aacggcatcg ctgagataaa
     1501 tccgtcacga atagccatga cgtggcgctg cgacgagatc cgccctgcta tggggcttat
     1561 gtaattttct acaaagcgaa atatcaaatt aaacgcagca tggttagcag acatagtagc
     1621 cctcctgaca ggctattgat actggcccaa caacgattac cctgccgtta cgatgaccgc
     1681 tttttgacgc agcaggccgc tggcggatgt gcaaccggct atactgctca tgaggcaacg
     1741 attcatacac ggctcttttg ttataaataa aaggagaagt gactgttcca gtattcatct
     1801 caatgcagat aaccgcaacc ggttacagta accggttacg gcgattgtga aggagatcga
     1861 caaaatagaa agaatctgga ttgtccgcgc tgatatttac actccgccag gcttgtgaca
     1921 gatttaacca ggcttaaatc aggagaatcc aatgagtctg cagtctgtac ggcagttttt
     1981 tgccgaacac gcccccgata tcgaaattat tgagttaaat cagagtaccg ccaccgtcgc
     2041 acttgccgct gccgcacaca acgttgagcc ggggcaaatt gccaaaacgc tgtcgctcaa
     2101 gatcaaagat aagattgtac tgatcgtcgc caggggcgat gcgcggctgg ataataaaaa
     2161 gctgaaagag acctttggcg cgaaagcacg catgctaagc agcgatgaag tggttacctg
     2221 gaccggtcat ccggtcggcg gcgtttgccc gtttggtctg gaaaatccgc tggcagtcta
     2281 ctgcgacgtc tctctcagac attatcagga agtactaccc gcagcgggag ccatccatag
     2341 cgcggttcgc attgaaccag aacgcatggc gcagctcacc gatgcaacat gggtagatgt
     2401 gtgtttatag ccgcattacg ctacctgttt aacggcgctg gtcgtactat tgagcggtaa
     2461 atgcaacagg atatccgccg cgtttaataa accaatgtct gagcgaactc cgagcttggt
     2521 catggcgtta aacttatgcg cgcggatcgt tttaatattc cggttgagct gtatcgcaat
     2581 atcagaaagc gaatatcccc acgacatata ccgaaggatg gcgtgctccg tcgggcttag
     2641 cggatcgttg cacgcctctc tgtcgcaaaa ttgttcgctg ccgctgagat gatgattgtc
     2701 cagtagctgc agcaaacgct cctgtagccc gcgtcgcggc gcggatttat tcagtatgcc
     2761 gtgcaaacag gccggcataa gatggccgat caggtgcatt tcactttcat tattagccag
     2821 aataatcgtt tggacttccg gccggcaccg cgccatttca tgaacaaaga gcaggctctc
     2881 ggctcgtgaa gaacgacttc ccgaaagaga aaaaataatg gttgaccagg gaaacggtat
     2941 ttccgccgcc ctgagcatac tgacattttt aaacagatga atatgataac ggcgattatc
     3001 ttcacgacta aagatatagc gccatgccat ttccgtcatc acgcattttt ctacgatggc
     3061 gacatggcgg gttgtactat ttttttccat tccatttgct ccccgtaagc tgaattgata
     3121 ttcagctctt gcattccctg cgcgctgttg atccaggcat acatttcagc gttgctgcgt
     3181 agagataagc gacgcattgc gctgtttttc tgcgcgctga tcgtcttatt actctttttc
     3241 aacagcgcgg cgatctggtt tattccccac cccttcgcca gaaggcgcag aactttacgc
     3301 tctgaaagag tgagcatcgc cgcagagtgg tagcttgcga aatcgctgtc ctcatccccg
     3361 gcaaacagcg tacggctaat ctgttcagcc gcacggctac cggcgcaaat cacctcaatc
     3421 agccggttaa ccggctcgct ctcggagatc aacgagcttt caggacgaat taatagctca
     3481 acggccaacg gatagaggct gtcgctgacc ataaaaaccc agtgaatatc acggtactga
     3541 gtcatgaggg agtaataacg ctcgcagacg gctcgggggt gcgcaatctc gccggagata
     3601 tccgcgacaa ccagcaccgc ccgccgcagt tgtaaaaggg tgagctcatc atgagaacga
     3661 caaaaactca gttcgtaatc agggaggttc tcattgaaga tcacctttaa ccctgattgc
     3721 atgacgggaa ttttactaat aacgacgcca aacttacctt gtcctggtaa cataacacct
     3781 ccgcttcctg tcgaagttcc ttcgcttgcc tgcaaacgat cggcgagcga ctttatcgcc
     3841 atatcatttc ctggatgaat attaaaatca tattcatggc ctgcgcacac gatatacgta
     3901 acgaccgaac cgtggtcata ccgtgtaaaa tattattgcc gtgtatactt gcgattaata
     3961 ttaacacaga cattcctgcg ctaataaatt agaaaagata aaaacattat ttagcaaaaa
     4021 taatttcagt actgaacatt atgaagcatt atctttaatt gtttctctaa atgctttggg
     4081 tgacataccg ctatagcgac ggaaaaaacg cgaaaaatag gctgcatcgc taaaccccaa
     4141 ataatcagag agttgtgtaa tcgtcatgcg ggtgtactgc agattgcgtc gcgcttcaag
     4201 catcaaccgc tgatgcaata ctccaagagc gctacagcca taaaactcac gacataaata
     4261 atttaagtgc gtaaccgaca gtccaatcag cttcgcatac tccgccaacg ggagatgctg
     4321 tcggtagtgg ctctcgataa ggcgggcgaa gtggcgcatc atgctgcgct tccgctccgc
     4381 tttatcttcc gcgggggacg cgggatggca ctgtcggttc agccagacca acagcgtccc
     4441 cagcagcgaa tagagcatca tttctcgcgc atcgtgctct tcgcagtact cctcgcgaag
     4501 cgctgaaaac agcgtatgca tgcgcccgcg agaacggccc accggcacac actgcggcac
     4561 gctaagcaca tccagagggc gaccgaactg attttcaaac cggctcagca gcggcagcgc
     4621 cagagagagc acaaaccctt gcgtcccggg tgaaaaatta aacccgtgaa tacagagggc
     4681 gggaaccacc tggatgcagg actccgtcat caccgcggtg gcgccttcaa tttcgatctc
     4741 cgcccgcccc tgatgcagat agagaagctg gaccatttcc gcatgctgat ggacgcgaat
     4801 atgccactga tacaggctgc tgcgctgtaa aatcgactca cagtgcagca ggtccggcgt
     4861 cggccagccg cactcttcgc catacagctt aaatacgggc acggtttgaa cgaacggcat
     4921 gatgactcct cttaattcag gcgacacgct ccatgggcaa gcccacgtag ttttccgcga
     4981 tggtggtcag cccggcgcgc gagcccagat agtagcggcg gtcggcggcc tgcattttag
     5041 cgtcgaacgc gttctgatcc ggaaaatcat gcagcaggcg ggtcataaac cagctaaagc
     5101 gttccccttt ccagacccga tccagcgcca gttgcgagta ggtcgccagc agatcgctgc
     5161 ggccacgatg gtagtattcg cgcagaatcc gccacagata gttcacgtcc gatgccgcca
     5221 gatttaatcc tttcgccccg gttggcggaa cgatatgcgc cgcatcccct accaggaaca
     5281 accgcccata ttgcatcggc tccacgacga agctacgcag gggggcaatg ctcttctcaa
     5341 gtgagtgacc agtcactaac ctggatgcca gctcctccgg caagcggctt ttcagctcct
     5401 gccagaagcg ctcgtccgac catgcttcaa ccttatcgct cagcggaacc tgcagataat
     5461 agcggctgcg agtcagtgag cgttgactgc acaatacaaa cccccgctga tggtgcgcat
     5521 aaataagctc cggattgacc ggcggcgtat ccgccagcag gcctagccag ccaaacggcc
     5581 acacgctctc gtaggtctgc aaaatgccag ctggaatact ttgccgcgat acgccgtgga
     5641 aaccgtcgca accggcaata aaatcgcact ccagacggca ggtttcccct tcgctgaggt
     5701 aggtgatcgt cggccggtcg cttttggcgt catggatagc cacctcgctc acgccataga
     5761 cgatcggcgc gccgctggcg gcccgggcag ccatcagatc gcgggtgact tcggtctggc
     5821 cataaaccat tacgctctta ccgtcggtta gctcactcag tgcgacgggc acccgctggc
     5881 cgtcaaacag aaactccacc ccatgatgca ccaggccttc ggcatccatc cgctgcgcca
     5941 cgcccgcttc gcgcagcaga tcaacggtac cgctttccag aattcccgcg cggatgcgcc
     6001 ccagcacata ctgcggcgtt tgacgctcaa ggatcacggt atggatcccg gcgttgtgca
     6061 gcaactgccc cagcagcagc ccggaggggc ccgcgccaat aatcgcgacc tgtgtcttca
     6121 tagggatgtc tcactcttgc agggttaatt atttgtataa tttttgttta ataaaagtgc
     6181 ttttctgcat ttttgataag tcagtgggga aaaagaaggg atgaatggcg acaaaaattg
     6241 cacttttcac caaactggca aaagtgtggg atgcttcaaa ttctgccgcc ccgcagaccg
     6301 ggaatttttg cacattattt gatccccccc ctcagccgcc actgcgcttc aggttaaagt
     6361 atcggcagat accaggcgtc gtggacaata gaggaagtat gcaggttgat cggacacagc
     6421 agcgggcaat cacacgttta tgcatccagt gtggtctttt tctgttacag cacggcgccg
     6481 agagtgcgct ggtggaggaa ctgtcaacac gtctcgggct ggcgttgggg atggacagcg
     6541 ttgaaagcgc catctcttcg aatgccatcg tcctgaccac cattaaaaat ggcgaatgtc
     6601 ttacctctac gcgcaaaaat gtcgatcgcg gcattaatat gcatgtggtg acggaagtgc
     6661 agcacattgt gatcctcgcc gagcataagc tgctggacta tcgcgacgtc gagaagcgtt
     6721 ttgcgcagat cgttcccctg cgctatccgc gctggctgct ggtgctgatg gtgggtctct
     6781 cctgcgcctg tttctgcaaa ctgaataacg gcggttggga tggggcgctg gtcagcttct
     6841 gcgccagcac ggtagccatg tacattcgcc aggtgctgac ccatcgctcc atgcatccgc
     6901 agatcaactt ctgcatcacc gcctttgtcg ccaccaccat ctccgggctg ctgctgcgcc
     6961 tcccggcgtt tgccagcacg ccgaccattg ccatggccgc cagcgtcctg ctgctggtcc
     7021 cgggttttcc attaatcaat gccgtcgccg atatgtttaa agggcatatc aataccggac
     7081 tcgcccgctg ggcgatcgcc agcctgctaa ccctcgccac ctgtattggc gtggtgatgg
     7141 ccatgaccgt atgggggctg cgcggatggg cataatctca tttatttttg cgctggcgga
     7201 agacatgctg ctggcggcga tccccgccgt cgggtttgcg atggttttta atgtccctca
     7261 gcgcgctctg cgctggtgcg cgctgctggg ggcgattggc catggctcgc gtatgatcat
     7321 gatgagcgcc ggttttaaca ttgaatgggc cacctttctc gccgctctgc tggtgggcag
     7381 cattggcatt caatggtcgc gctggtatct ggcgcatccg aagattttta ccgtcgccgc
     7441 cgtgatcccg atgtttcctg gcatctcagc ctacacggcg atgatttccg cggtaaaaat
     7501 cagtcatttt ggctattcgg aagagatgat gattttgctg ctgagcaatt ttttaaaggc
     7561 ctcttcgatt gtgggcgccc tttcgattgg cctgtcgata cccggcctgt ggctgtatcg
     7621 taagcgtcct cgcgtctaac cccgacgtcg cggttaatcc gcgacgctcc ttccgcgatg
     7681 gcctctgtgc tactatgtgc agatcacccc actctttgca gttcagagtt gagatagagt
     7741 tatgtcttcg cgaattttaa cctcccattt tagcggcctg gaagagttcc tccagcagca
     7801 cgcagcgctg ctggctaaat cgactgacgg caccgttgcc gtttttgcca ataacgcgcc
     7861 ggcattctat gcgctgacgc cagcgcggct ggcgcaactg ctggagctgg aagccaggct
     7921 ggcgcgcccc ggcagcgaca tcgcgcttga cccgcagttt ttcgaggagc ccgccgcggc
     7981 gccggttgcg gtgccgatgg gcaaatttgc tatgtacgcc gggtggcggc ctgacgccga
     8041 ttttcagcgc ctggccgcgc tgtggggcat tgccctgagc cagccggtta cccccgaaga
     8101 gctggccgct ttcgtcgctt actggcaggc ggaaggtaaa gtctttcacc acgtgcagtg
     8161 gcagcagaag ctggcgcgta gcgtgcaaat cagccgcgcc agcaacggcg gacagccgaa
     8221 gcgcgatgtg aacagcgtta gcgaacccga cagccatatt ccgcgaggtt tccgaggatg
     8281 agcatgaaaa acgttggcga cctgatgaaa cgcctgcaga aaatgatgcc ggcacatatt
     8341 gaaccggcgt ttaaaaccgg cgaagagctg ctggcctggc aaaaagagca aggccggctg
     8401 cgttccgaag cgctggagcg agagaatcgc gcgatgaaaa tgcagcgcac cttcaaccgc
     8461 tcggggatcc gcccgctgca tcagaactgc tcctttgata actatcgcgt cgagtgcgag
     8521 gggcagatga atgcgctcgc ccgggcgcgc cagtacgtgg aagagttcga tggcaatatc
     8581 gccagcttta tcttctccgg taaaccgggc actggcaaaa accacctggc ggcagccatt
     8641 tgcaacgagc tgctgctgcg cgggaagtcg gtcttaatca tcaccgtggc cgatattatg
     8701 tcggcgatga aagatacctt cggcaaccgg gaaacgagcg aagaacagtt gcttaacgac
     8761 ctcagcatgg tcgacctgct ggtgatcgat gaaattggga tgcagacgga gtctcgctat
     8821 gaaaaggtca ttatcaatca gatagttgac cggcgctcat cctccaaacg acctaccggg
     8881 atgctcacca acagcaatat ggaagagatg aacaaactgc tcggcgagcg ggtgatggac
     8941 cgtatgcggc tcggcaacag cctgtgggtt atcttcaatt gggaaagtta ccggcaccgc
     9001 gttacaggca aggagtatta agagatttcc gggcgacgac ggcgttatac taaagcgatt
     9061 attcagtcac gcttatgagt ccgtctatga aatcgtttaa aaatagcgtt attgccgcgc
     9121 ttgccgttag cgcgtttacc ctctctggcg cccacgccgc ctcctggcag gattccctta
     9181 acagcgctgc cagccagctc agcggcagta acgcccaggc cagcggcggc atgtccgtct
     9241 cttcgctaac cagcctgctc aacggcagca gccaatccct gaccgccagc agcatgaata
     9301 atgcggccgg gatcctgtca tattgcgcga aacagaagct ggcctccgtg accagcgccg
     9361 ataacgtcaa aaatcaggtg ctggataaac tggggcttag cacgccggaa aaacaaaaac
     9421 aggacaccag ctatctggat ggcctgcagg ggctgctcaa cagcaaaaac ggccagcagt
     9481 tggacctgaa tacgctcggc aatagctcgc tggcgaaaca ggtaaaaacc aaagcctgcg
     9541 atctggtctt aaaacagggt gttaatttcc tctcctgatc ctgactgaca aggccgcatt
     9601 tgttgccttc gcggcggcgc cgcggcctga aacgcttttt tttgcagttt tcctgttccg
     9661 ttttcccatt tcgcccgatg cgatgccgat caaaaaaggc aacttctaaa ccaattctga
     9721 ataactgcac actttcatga cgctagaatt gcagcaagat ggcgctgcaa ttacagtggg
     9781 gcttgaaaag taatctgttc tgccagccga taactggcat taatgaggat gagctgtgtc
     9841 agagttgtta tccatcgcct tatttttagc ttcggtggtg ctgtatgcct ggaaagcggg
     9901 tcgtaacacc tggtggtttg ccgccacgct cacggtgtta ggcctgttcg tggtgctgaa
     9961 tatcaccctc tacgccagtg actacttcac cggcgacggc atcaacgacg cggtactcta
    10021 cactctgacc aacagcctga ccggcgccgg gatcggcaaa tatatactgc cgggggtcgg
    10081 cgtggccgtc gcactggtcg ccgtctttgg cgccctcggc tgggtgctgc gccgccgtcg
    10141 gcatcatcct catcacgtcg gttacagcct ggcggcgcta ctgctggcgc tggcctccgt
    10201 tgatgccagc ccggccttcc atcagatcag cgagctggtg aaatcccagt cacgcgaggg
    10261 cgatccagac tttgccgcgt actataaaga gccgtcgaag caaatcgaca atccgcagct
    10321 gaacctggtc tatatctatg gcgaaagtct cgagcgcacc tattttgata acgacgcctt
    10381 ccccaacctc acccctgagc ttggcagaat taaagatgag gccattgatt tcagcaatac
    10441 catgcagctg ccggggaccg attacactat cgccggcatg gtcgcctcac agtgcggcat
    10501 tccgctgttc gcgccgttcg aaggtaatgc ctcagcctca gtctccagct tctttccaca
    10561 gaatatttgc cttggcgata ttctgaagaa ctccggttac gagaattact ttgtgcaggg
    10621 ggctaacctg cgctttgccg gcaaagacgt gttt
//
