LOCUS       NZ_CP079119             5251 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC01_S465PC plasmid
            pS465_P4, complete sequence.
ACCESSION   NZ_CP079119
VERSION     NZ_CP079119.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20115014
            Assembly: GCF_019286375.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC01_S465PC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC01_S465P
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:04:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,543
            Genes (coding)                    :: 5,402
            CDSs (with protein)               :: 5,402
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 61 of 141
            Pseudo Genes (incomplete)         :: 75 of 141
            Pseudo Genes (internal stop)      :: 24 of 141
            Pseudo Genes (multiple problems)  :: 17 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079119.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC01_S465PC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pS465_P4"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            complement(77..289)
                     /locus_tag="KWK18_RS28175"
                     /old_locus_tag="KWK18_28175"
     CDS             complement(77..289)
                     /locus_tag="KWK18_RS28175"
                     /old_locus_tag="KWK18_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(293..778)
                     /locus_tag="KWK18_RS28180"
                     /old_locus_tag="KWK18_28180"
     CDS             complement(293..778)
                     /locus_tag="KWK18_RS28180"
                     /old_locus_tag="KWK18_28180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<816..1493)
                     /locus_tag="KWK18_RS28660"
                     /old_locus_tag="KWK18_28185"
                     /pseudo=""
     CDS             complement(<816..1493)
                     /locus_tag="KWK18_RS28660"
                     /old_locus_tag="KWK18_28185"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1456..1776)
                     /locus_tag="KWK18_RS28190"
                     /old_locus_tag="KWK18_28190"
     CDS             complement(1456..1776)
                     /locus_tag="KWK18_RS28190"
                     /old_locus_tag="KWK18_28190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2413..2574
                     /locus_tag="KWK18_RS28195"
                     /old_locus_tag="KWK18_28195"
     CDS             2413..2574
                     /locus_tag="KWK18_RS28195"
                     /old_locus_tag="KWK18_28195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     gene            complement(2727..3347)
                     /locus_tag="KWK18_RS28200"
                     /old_locus_tag="KWK18_28200"
     CDS             complement(2727..3347)
                     /locus_tag="KWK18_RS28200"
                     /old_locus_tag="KWK18_28200"
                     /inference="COORDINATES: protein motif:HMM:NF025084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(3340..4695)
                     /locus_tag="KWK18_RS28205"
                     /old_locus_tag="KWK18_28205"
     CDS             complement(3340..4695)
                     /locus_tag="KWK18_RS28205"
                     /old_locus_tag="KWK18_28205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_014386473.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     Typing          complement(1891..2063)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          2010..2523
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(4..1466)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.107"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 gtttccgcca gctctttttc atgctgtttc tgctcgtatg cccgttcggc ggccaccgcc
       61 atttcatgcc gctgcactac tggctcaaac gctgtaactg cctgctcaag ttcgccacct
      121 gctgactcaa gcgcgtgacg cgctcgttca gcgccgcgtt ctcccgttgc ataagaccag
      181 acatcttctg ccattccgcg aaggcgctct cccactcgtc cagcctttgc gagtagtcct
      241 gatgtagctg ctctaatgcg gtcagcaact gtttttccag ttccgtcatg ggctatttcc
      301 tctccagtac catccagttc ccttcctccc cgtaagctcc cgctttcggg ttcactttga
      361 cgcaggacag tttcttctcc ccgcagagcg acagctgcac gccgtggttc ttctccgaca
      421 gcatggcctg cgtccgctcc tgcgcccgga tagtctggta attcccggtg atctgctgcc
      481 cctgccacca cagaatgctc ccgctcgtgg cgatcagcag taccgagacc agggtgatgg
      541 tcagccacgt ccggccgacc atacgcagca cgctcagccg gttcgactgc atcgccgcgg
      601 tcataccctg ctcgtgggcg ctgatggcgt cgctgattct cttcgcgctt gcgctcagtt
      661 ccgcttttac agactgctcg tgctcgctga atgcggcttt cagcatctcg ccggtactct
      721 gctgctgcgc tttcgattgc tgctccaagt ccttcgccag cgctaaaaga ctgctcataa
      781 atggctccct tcagtcggat gtttcgcccc ccgtccgggt cggcaatgct gatactgttt
      841 tttgtggtgc ggaccacctc aaaacctgcc gctgtgagcg cccctgtgac gtcctcgcgc
      901 gttttgagct ccccggatga ggctaaggcc agcaacccac gtgtaatcgc ctgagcggct
      961 tccagcttcg tttccggcag tgcggacggc gtgaccagta accgtcggtt ctccggggcg
     1021 tttggatcat gcagccccag cctgccgttc accacggtct gccaggcatc gatgcgcggc
     1081 cggtcggcgc gatcgtagta cggctgcagg cgtttcccgg tcagcagttc agtgttgggg
     1141 atcaggaaat tcagctccag ccgccccttg tcggtgtgct ccacccacag aatgctgtac
     1201 tggtctttat cgagcccggg catcagaacc cgctcaaagc tggccatgat ctgctcccgc
     1261 tgcccgggcg gcagttcagc ctccgcgaac gacagcacgc cggaggtgta ctttttcgca
     1321 tacggcgagg catcgataag ctcccggact tcctccggtt ttccctgcag cacggtcgcc
     1381 ccctcgcgct gccggtcttt tcccagcagg taatccaccg gaccggcacc accaccgcgc
     1441 ccccgggggt gaaatttaac gatcatcatc cccccctttt tccagcaccg catgccgcag
     1501 ccgctccagt cccgcgtcga ttgccatcag tgcggccaca atctgcaccc ggtcatgacc
     1561 ggcaccaccg gcattcactt tccgggcgat ctggttcagg ttgttcccca taccggcaag
     1621 ctggcgcagc agcgccggcg atatcgacgg cagtctgccg gcacgcgccg gcttctcatc
     1681 gaggcaggtc tgacgcatcc acgccgccag ctgcttgccg tcgcaccgct cgagcagccg
     1741 ccggtgttcg tcttccgtca cccacatcgt gagcatcttg ttgcgtttgt ctgccagagt
     1801 agcctcctga taacaccggg acgggcggga aatgtttccc ggtcggcacg gtgttggaca
     1861 agccgcaggc gcgtcagtcg cttttagcgg gtttcggggc gcagccctga accagtcacg
     1921 tagcgctagc ggagtgtata ctggcttatt atgttggcat gagtgcggta atcaggaaag
     1981 tgcttcatgt gacagaggaa aatgcagctg cgggcgtaag gcagaaaatg gaagttcaga
     2041 ggatatgacg cctcctcgct cactcagtcg ctgccgctcc tgccgttcgg ctgcggcggg
     2101 cgttacctgc acacggagaa gagcgaaggc atatggcatg acaaacgggg cagaaaaagg
     2161 atgcgccgct gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     2221 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2281 gtggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     2341 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     2401 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt ctccggtaac
     2461 tatcatcttg agtccaaccc ggaaagacac ggttcatcgc cactggcagc agccattggt
     2521 aacggattag aggaagtgag tcttgaagtt ctgcgccagt taaggctaaa ctgaaaggac
     2581 aatttttggt gactgcgctc cctctaagcc agttacccgg tacataagtt ggtagctcat
     2641 gtaccttcca aaaaccaccg ttggtagcgg tggtttttat ttttcataga ttacagcctg
     2701 taataagcca tggtcaatgg attacttcaa aagtcgtcaa tcgccttaag tagctcatgg
     2761 aatgatgtat aacatggaaa tagataaggt tcgtgcgtgt ttttataatt agttttagcg
     2821 taaatatcta gctttcttgc gttatctttc gcagtaccaa tcctctctct tatagcaagg
     2881 taagttagtt tactaccttt atcatagtta gttattaatt tttttagcgg ggagctagac
     2941 atcaaattat catacgacga aaaatctttg gttgtgaagc cgaaatgcaa caataaccat
     3001 aattcaaaac aaacacagga tattgctata tttatcccat gcctattagc tttattccat
     3061 gccttttgat gacatgaaat agacgaaggc gtcagatgct cttgatcata aacaacccaa
     3121 aagacgtcac cttctgcgga agatgatgaa ttctttttct ttatcgcctc atccaccaac
     3181 tgctcaggtg tatttttcct tgttttaggt attttaaata cacttaaagt tcttttagat
     3241 ctgtctgaaa tataagcctc aaggtataat ggttcggttt tctcaccctc acagtatata
     3301 tacattactt gtgggacttt cttattattt ccatgttttt tactcatcag aaagatcccc
     3361 gtcaaactct tcaaaaaaac catcagacaa ttctttgacg tcaatatccg gcattaccaa
     3421 ggatatatca ccagatataa atgaggctac ctttaagtag tttatgtcgg gaaccccacc
     3481 aaacctaccc tcatcatacc aagctgcgta aggtgttgtt gtttttatct ttttcttatc
     3541 aaaatcatct aagctataaa gagacgacgc acctttattt ttttcacaaa accatatctg
     3601 atctcttctc attttctctg gagccattaa ctgcatatta tgggttgaga atacaagttg
     3661 tgagcctttt ttattgacat caggatcatt aaataactta acaatcaagt cagcaatatg
     3721 cgggtgcaag ctattatcta actcatcaac aattacaacc atactatcat taaacgcaga
     3781 tctaattaga ggtatgattt caaataattt ttgggtgcct tcagactctc tccccatagg
     3841 gaaagaaaca ctcccccctt catcagtagg atgagagaac atataacgat atttataatc
     3901 ttcttcaatt atctccttta tttcttgagg gatttcctca tccattttta ttggtatttc
     3961 cgcctcaacc tcccgtacag atataccgga aacaccagtg tcaaccatgc ataaaacctt
     4021 ggccgtttca tttacgacgt cctccctctc cccaaaagaa gatattctta tcttttcatt
     4081 taaacctata tgtctaattc cctttctaaa aaagttataa gcttctttaa ttatgtctgg
     4141 tgatgcggca ttattacctg ctttcgctaa gtatgaatta ttagggaaga atggtatttt
     4201 tttaatacct cctttataat gcccaccaaa cttgattgtt tcccaggtgt cgctctcatc
     4261 tctagtaaat aaatttgctt taaccctaga tggataatag tctaatgatt caaataaaat
     4321 ccgattttta ataaaagcaa cttcataact gaaccgattc ccctcaacag aataaaattc
     4381 agcctcaaac tttacaggag cctcattcgt agccgaagaa agagcatatg gctcatagca
     4441 gggaatccgt ttaccttctt tcaaatcacc gctttctgtt gcgataaatt ttatagcttc
     4501 aaaagccatg agaacgttag atttaccaga cgcattagca ccataaatcc cagcactcat
     4561 gagcgttcca acattcattc catcattttt atagatatga gttgttaaat ggttactaga
     4621 accggatgcg ctcaagttta atgtcgattt ttccttaacc gacctaaaat tttcaatagt
     4681 caaataaaca aacatgcata cctcctaacg gggatttttc gccaaaaaag cgaaaaatca
     4741 atcataggaa agatatccta tatgttttaa ttttggaaca acatcttcct taaaaaacgt
     4801 tgtgctattt ataattacac gctatttacc ataacatctg ttgcccgcac cgctccggac
     4861 aggttcgcca caaacggggc ggcagtcagg taaaaccggc aacaaaccac ccgatacacc
     4921 gccccgttcg tgacagataa attttacccg gctgcatggt catgcagcca gatgcatacg
     4981 gtctgatttc aggccagaat ccggatgaac gccttaaaaa caggaaaatg cttctccagc
     5041 cagtcccaga tctccagcgc catgtacagg caattcagca ccgtctcaat cacgttaaac
     5101 agtctcataa tccctccgtt gtatacgctg tgatggaaac atcacgaccg tatttagcgg
     5161 gaaatttatg agcccttctt cttggggttt gtcactggat gcgcgcagtc agcccagcgt
     5221 cggaccgtca tagctccgct gccgggaacg g
//
