LOCUS       NZ_CP078128             5596 bp    DNA     circular CON 11-FEB-2024
DEFINITION  Klebsiella pneumoniae strain KPC-2 plasmid pKP169-P6, complete
            sequence.
ACCESSION   NZ_CP078128
VERSION     NZ_CP078128.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19312573
            Assembly: GCF_019444195.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Huang,Y.
  TITLE     Rapid detection of KPC-producing Klebsiella pneumoniae in China
            based on MALDI TOF MS method
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Huang,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2021) Department of Clinical Labortory, Renmin
            Hospital of Wuhan University, Zhangzidong Road, Wuhan 430060, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 04:05:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,179
            CDSs (total)                      :: 6,055
            Genes (coding)                    :: 5,851
            CDSs (with protein)               :: 5,851
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 79 of 204
            Pseudo Genes (incomplete)         :: 136 of 204
            Pseudo Genes (internal stop)      :: 25 of 204
            Pseudo Genes (multiple problems)  :: 35 of 204
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP078128.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPC-2"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP169-P6"
                     /geo_loc_name="China:Wuhan"
                     /collection_date="2020-12-28"
     gene            complement(join(5488..5596,1..116))
                     /locus_tag="KU889_RS30600"
     CDS             complement(join(5488..5596,1..116))
                     /locus_tag="KU889_RS30600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(783..995)
                     /locus_tag="KU889_RS30605"
     CDS             complement(783..995)
                     /locus_tag="KU889_RS30605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1002..1511)
                     /locus_tag="KU889_RS30610"
     CDS             complement(1002..1511)
                     /locus_tag="KU889_RS30610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1546..2250)
                     /locus_tag="KU889_RS31285"
                     /pseudo=""
     CDS             complement(<1546..2250)
                     /locus_tag="KU889_RS31285"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2189..2533)
                     /locus_tag="KU889_RS30620"
     CDS             complement(2189..2533)
                     /locus_tag="KU889_RS30620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2523..2699)
                     /locus_tag="KU889_RS30625"
     CDS             complement(2523..2699)
                     /locus_tag="KU889_RS30625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3339..3497
                     /locus_tag="KU889_RS31290"
     CDS             3339..3497
                     /locus_tag="KU889_RS31290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            3855..4181
                     /locus_tag="KU889_RS30630"
     CDS             3855..4181
                     /locus_tag="KU889_RS30630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4207..4752
                     /locus_tag="KU889_RS30635"
     CDS             4207..4752
                     /locus_tag="KU889_RS30635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            4818..5168
                     /locus_tag="KU889_RS30640"
     CDS             4818..5168
                     /locus_tag="KU889_RS30640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_149795812.1"
                     /translation="MYWLITFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(5238..5504)
                     /locus_tag="KU889_RS30645"
     CDS             complement(5238..5504)
                     /locus_tag="KU889_RS30645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(2786..2862)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          3051..3448
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(668..2199)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcttccagat gcgccagtat tgcttcccgc gcataaaacg tctttgttct gccggtttgc
       61 ttcgccagcg agtccagacg ggactcaatc tcatcagata accggatagc aagcatataa
      121 acctcccgaa caccatttgc tatacatgta tatcaacagt accacaatca acctaaaaga
      181 acaaaaaata atcttgttag cccctcttga taatccgtaa cgcggtcaat atatctagct
      241 tgagggcttt cctcttaact attgaaagga taacgattat gggtcttaag ccgggtccga
      301 aacgaattgc cgattcaaca ggggaacctg ataaacgcca gcgtgacaat aaaaaaacgc
      361 cgggaaatac tgacaaattg aaacccagca aatcttcaaa aaaataagtc agtagttaaa
      421 agccctccgg ggcttttttg tatctgccgt tcacgctgat taaacataac acccgttgcc
      481 cgcaccgccc cggacgggtt cgccgcgaac ggggtggcag tcaggtaaaa ccggcaacaa
      541 accacccgat aaaccgcccc gttcgcgaca gataaatttt acctggctgc atagccatgc
      601 agccaggtga atgacatccg gtttcagcgt aaatgcccga tgagaaccct gttttcctgc
      661 atttcagccc agcgacggac cgtcatagct tcgctgccgg gaacgcgctt ccgtcagttc
      721 cttttcgtgc tgccgttgct cgtgcgcccg ttctgcggcc acggccatct catgacgctg
      781 cactaccggc tcaaacgctg cagttgcccg ctcaagcgtt ccacctgctg actcaagcgc
      841 gtcacgcgct cgctcagcgc cgcgttctcc cgttgcataa gccctgacat tttccgccat
      901 tccccgaagg cgctctccca ctcgtccagc cttttcgagt agtcctgctg tagctgctct
      961 aatgcgctca gcaactgttt ttccagctcg gtcatcggca cttactcctt caccagtctc
     1021 agcgcgttct gcttcgaccg cccgttcccg accgtccagc tctgcgtcct gtctacggcc
     1081 gtccccttcg gcagcaccag aaaacgtccg ttgctgtctt ccaggtacgt cacgccccag
     1141 gtcttcgcgt tcagcttcga cagcgctgcg ttctgccggt cgatttcggc cagatttgac
     1201 gctatcaagc tcccctgata ccacaacacc ccgctcaggc tggcgaacag cagtcctgac
     1261 accatcgtga tggtcagcca tgtccgcccg accatacgca tcacgctcag gcggttcgac
     1321 tgcatggccg cgctcatacc ctgctcgtgg gcgcggatgg cggcgctgat tctcttcccg
     1381 ctttcgctca gttccgctct gacagacttc tcgtgctcgc tgaatgcggc tttcagcatt
     1441 tcgccggtac tctgctgctg cgctttcgat ttctgctcta agtccttcgc cagcgttaaa
     1501 agactgttca tagatggctc ccttaagtcg gatgtttcgc cctccgtccg ggtcggcaat
     1561 gctgatgctg ctctttgtgg tgcgcacgac ctcaaaacct gcgctttcca gcgcctcagt
     1621 gacgtcctga cgcgttttaa gctcccctga cgaggcgagg gccagtaagc cccgcgtaat
     1681 agcctctgcg gcttcctgct tcgttttggg taacgaagat ggcgtcacca gcgcgcgccg
     1741 gttctccggt gcgttcgggt cgtgcagccc cagtcgcccg ttcaccacgg tctgccacgc
     1801 atcgatgcgc ggacggtctg cccggtcgta gtacggctgg agacgcctgc cggtcagcag
     1861 ttccgtgttc ggtaccagaa aattcagttc cagccgcccc ttgtcccggt gttcaaccca
     1921 cagcacgctg tactggtctt tatcgagtcc gggcatcaga acccgctcga agctcgccat
     1981 cagcttttca cgctggccgg gcggtaaatc ctgttcagca aaagacagga cgccggaggt
     2041 gtactttttg gcgtagggcg aggcgtcgat aagctcccgg acctcctccg gctttccctg
     2101 caggacgatg gccccgtcgc gctggcggtc tttccccagc agataatcca ccggcccggc
     2161 accgccgcct cgccctcgcg gatgaaactt aacgatcatc gtctgccccc ttttccagca
     2221 cggcatgccg cagccgctcg agttcggcat caatggccat cagcgcggcc accacctgaa
     2281 cccggtcagc gcctgaccac tggccgccgt taattttccg ggcgatctgg ttgaggttgt
     2341 tccccatccc ggcaagctgg cgcagcaaaa ccggatcgat ggacggcagc cgcaacgacc
     2401 gcgccgggcg cgtatcgaga caggtttccc gcatccacac cgccagttgc cttccattgc
     2461 agcgctccag cagctgctgg tactccccat cggtcacccg gatacctaaa aaacgggtac
     2521 gcttaaccgc catcctctcc tccgatatcc gcaaaatgtg accgggctga acgctcaagc
     2581 tctgccatct gctcccggcg gtaatcttcc tcctgtgcct gacgcatcag atttgccatc
     2641 actgccggat gccaggatct tttttttacg acgagcggct tcttcttcgg cttctgcatt
     2701 atccctcctt cgcaaacgcc ggacggcacg gaaaagtttt ccgggtcgct ccggcgttgg
     2761 acaagcgcca gcgcgtcagt ggtgttagcg ggtttcgggg cgcagccctg aaccagtcac
     2821 agcgtttacg ctgtgtatac tggcttacta tgttggcatg agagcacgat tcatgaaagt
     2881 gcctcatgtg gcaggtgtga tatgtggctc aggagcgtcc gcaataccat gcacggacag
     2941 atgaaatgac gcctcctcgc tcactcggta gctgcgctcc tgccgttcgg ctgcggcggg
     3001 cgtgaccgac tcacggataa cgggataaag caaaggcgct caaatcagaa agggccggag
     3061 aaaaggatgc ggcgttgccg tttttccata ggctccgcct cccctgacga gcatcacaga
     3121 aatggacgct caagtcagag gaggacgaaa cccgacagga cttaaagatg ccggtgattt
     3181 ctctccggcg gctccctcgt gcgctctcct gttccgaccc tgccgtttac cggatacctg
     3241 ttccgccttt ttccctttcg ggaagcgtgg cgctttctca tagctcacgc tgctggtatc
     3301 tcggctcggt ttaggtcgtt cgctccaagc tgggctgtat gcacgaaccc cccgttcagc
     3361 ccgactgctg cgccctttcc ggtaactatc atcttgagtc caacccggac ggacacgcaa
     3421 aaacgccact ggcagcagcc attggtaact ggatatatac gagagagata ttgagaggtc
     3481 ttgaagtggg tgcctaactg cggctacact gaaagaacag ttttggtatc tgcggtctcg
     3541 tatacccagt tacccggtta agcagttccc caactgactt aaccttcgat caaaccgcct
     3601 ccccaggcgg ttttttcgtt tacagtgcaa gagattacgc gcagaaaaaa aggatctcaa
     3661 gaagatcctt taaatgatcg tcagttctgt tatccccgga gctccatcca tcccacggaa
     3721 aaacacccgc cactgatgcc ctcagcgcac tgcacatcat gtacaaacca ctccctccct
     3781 ccttttttat tgcgtttctt aatgcgcaat ggtagctttg tgcgcaacaa aaaacgcaat
     3841 agcaaggggg ggtaatgaga gaaagaacaa atatcatgct agatatagga ttaaagcaat
     3901 ccttacaacg gctttcgaat aaaaccaaaa tgagtctaag tgatcttatt aaccagagat
     3961 tatcaggttc actttcagtt gaagaacgca ttggcgaaaa gcctgaatgg atgagaaacc
     4021 ttgatcccta tctcgccaat cacttttcaa atattgagtt accgctccct tcaccattaa
     4081 aagaatacca agaaaaaatg gatgagatag attcaattat taatgacatg atggttctaa
     4141 aggaatattt cagagctaag ttttacgacg aaataaaata ggcacgctat acggaggcgg
     4201 agaattatgt tttttaaacg gtatttaacg gagaaggaaa tatacaaggc agcactccca
     4261 attgttaggc ttcaagggag agttaacact tcattacgtt ttttatccaa atactcagat
     4321 gtaaacaaaa atggattaat tgcattgcat cttaatttat tacattttat ctcatacaac
     4381 atacacggtg tagagtctct gaaaatcact caagcaggca taaacaaagc tgtacaacaa
     4441 agccctcacg cagatgaaat aaaaaatatt gcgcaggcaa taggacgcaa aggacctgag
     4501 tacatcgaag gattaattag aaacccatgg gggaagaaaa atgataatcc actaatattt
     4561 ataatgagca ttgaagaaag tataaaagaa acccttaact caggttatat gttatataga
     4621 gtagctccca taactgaggg tgaagcagct gctttcatgg atgttcttaa acaattatta
     4681 ttatttgagg ataattttcg cctttttggt agagacttgg tttataaaat agaaaaatta
     4741 aaaatcagat aacaaagtat ggtgaacgaa aaatgaaaga gaggatagtg aaatgtgtaa
     4801 taccaagatt ctcaactatg tattggttaa ttacgttcct agcatttata ttccccggtc
     4861 gatcttttgc tgacggttac accgcttact accgatgtaa tggtactcag gaagcatctc
     4921 ttactgttaa agataacaaa ctaagtatgg ttgtaggtct ttctaagttt aataggcaac
     4981 tggatactta tagattcaaa gaaaagggaa tcgaagtagt aatgcagaga gctgcttctg
     5041 atgataactc tgaggccata atgtctttta ttatgcctga cgttaacaat atgaccttat
     5101 tagttaaaaa taacggctac attatcgtta agacaacctg ctggttaact aaaaccgaca
     5161 aacagtagaa cacgtagtta gtcgagtatt taccataata cctgttgtcg gcatcaaaaa
     5221 agcccgcata gcgggcttca gtcgtaaact tcgcggcgat gtccaatcgt ggcggcaagg
     5281 ataacaagct cgtcatctcg gatctcacac agaacgcgat aatctcccac ccgatagcgc
     5341 cagaactcgc ccagctcacc tttgagcggc ttccctgact ggcgaggatc ggcagcaact
     5401 gcaatgcgta aactcataaa atccacaatc cgtcgtgcgt tctgtttgtc catcttgcgt
     5461 aacgatttga gcgcccgatc ggaatagtta atcgtccaga ccaagtgact tcctcacgtc
     5521 ttcagacgaa tgcactgctt catcaccacg gcgaacgcgt gcagcagttt ctgctgaaag
     5581 ataataatcc tccagg
//
