LOCUS       NZ_CP078011             2953 bp    DNA     circular CON 13-OCT-2023
DEFINITION  Pseudomonas aeruginosa strain FAHZU31 plasmid p3, complete sequence.
ACCESSION   NZ_CP078011
VERSION     NZ_CP078011.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19950563
            Assembly: GCF_019857645.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Zhu,Y., Chen,J., Shen,H., Chen,Z., Yang,Q.-w., Zhu,J., Li,X.,
            Yang,Q., Zhao,F., Ji,J., Cai,H., Li,Y., Zhang,L., Leptihn,S., Hua,X.
            and Yu,Y.
  TITLE     Deciphering plasmidome of KPC-producing Pseudomonas aeruginosa
            reveals the blaKPC-2 copy number correlates with
            ceftazidime-avibactam resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Zhu,Y., Chen,J., Shen,H., Chen,Z., Yang,Q.-w., Zhu,J., Li,X.,
            Yang,Q., Zhao,F., Ji,J., Cai,H., Li,Y., Zhang,L., Leptihn,S., Hua,X.
            and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, Zhejiang University, 3 East Qingchun Road, Hangzhou,
            Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/13/2023 05:46:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,286
            CDSs (total)                      :: 6,207
            Genes (coding)                    :: 6,141
            CDSs (with protein)               :: 6,141
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 43 of 66
            Pseudo Genes (internal stop)      :: 6 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP078011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="FAHZU31"
                     /host="Homo sapiens"
                     /db_xref="taxon:287"
                     /plasmid="p3"
                     /geo_loc_name="China:Hangzhou"
                     /lat_lon="30.28 N 120.15 E"
                     /collection_date="2017-12-10"
     gene            complement(join(2783..2953,1..381))
                     /locus_tag="KUU74_RS31305"
                     /old_locus_tag="KUU74_31300"
     CDS             complement(join(2783..2953,1..381))
                     /locus_tag="KUU74_RS31305"
                     /old_locus_tag="KUU74_31300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA plasmid replication protein"
                     /protein_id="WP_033995415.1"
                     /translation="MQQITVATDAQRRIRQRELAMLDEKEAQEREEARKNKGFTQVYPL
                     GWKRIIELAKGNAGAFGLYSFFAENIDPTCGAVVCDQQFLADKMNVNRRTISRWLSYLE
                     ENRALVRIPVAGKVCAYALDPHEVWKGYDNAKDYAAFVTKTLVNKDGDIRRRIMSMFSG
                     EKQNDDRDPNTIDMFESTPQ"
                     /name="RepA plasmid replication protein"
     gene            1435..2409
                     /gene="mobV"
                     /locus_tag="KUU74_RS31310"
                     /old_locus_tag="KUU74_31305"
     CDS             1435..2409
                     /gene="mobV"
                     /locus_tag="KUU74_RS31310"
                     /old_locus_tag="KUU74_31305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_033995413.1"
                     /translation="MAAYAIMRCKKLATMGSVAAALQHCYRERETPNADAERTPDNEHR
                     AATSTDAAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWAKATPQQQAAFFDQAQGWLA
                     AKYGADRIVTASIHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRDKMRADQTSFAEAVR
                     DLGLERGIEGSRATHQRVQSYYGAIERQPGHATITPQAIEPRVLRKGIFSKDVETPEAV
                     AARVTAAVREGYGPTVAAAAGARQEREKARQAQETARSLRDRLKPVLDALGPLNRDMQA
                     KAAQIIKAVGEKLLAEQREATRQRQAQRLRSRGRDRDDGGLSR"
                     /name="mobV"
     gene            complement(2436..2786)
                     /locus_tag="KUU74_RS31315"
                     /old_locus_tag="KUU74_31310"
     CDS             complement(2436..2786)
                     /locus_tag="KUU74_RS31315"
                     /old_locus_tag="KUU74_31310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="WP_077265610.1"
                     /translation="MSNETKEKIFMKKTFALAAMMAAIAPNLASAADEMIQTKYACEGG
                     KTLDVVYVGDYAVMLQMDELVPLKRAKSASGVRYISASKNYTYELWGKGNDMNLSTHDG
                     GKEQTVLSSCKP"
                     /name="MliC family protein"
ORIGIN
        1 ctcgtgagga tccagcgcat aggcgcaaac cttgccagca acagggatgc ggacaagcgc
       61 gcgattttct tccaggtaag acaaccaacg gctgattgtc cgcctgttga cgttcatctt
      121 gtctgccaaa aactgctgat cgcagaccac cgcgccgcag gttgggtcga tattttcagc
      181 aaagaatgaa tacaggccga aggccccagc attgccttta gcaagctcaa tgatgcgctt
      241 ccaacccaag ggatacacct gcgtgaaccc cttgttcttc cgcgcctctt cgcgctcctg
      301 agcctctttc tcgtccagca tggccagctc tcgctggcgg atacgacgct gggcgtcggt
      361 cgccaccgtg atctgctgca tggggttcct ccaaagtcgg gaaatttgtc cgctcattgc
      421 gccatttcga gtcgcaatga tacgacaaat tatgtctcac gtcgagacag tttgcgtctc
      481 aaccttgcta cctgttgaga catccatgca tgtctcaacg ttaggacacc ctatgtgtct
      541 caatgcattt ctacaaatcc ttataaatca ataacttagc atttccccct ttctcctcta
      601 aggggctgcc gccccgccgg cggccgctac gcggccttgg caccccgctg acggcccctg
      661 cggggcctct ggaaaaggcc cacccggcca ccggccgggt gacgttgttt ggggggcctc
      721 cggcggccgc tgcgcagcct tggggcggtg ggcgctgccc cccgccaccc cccgcgttcg
      781 ccacgtggca ggggtgcagg ggctacgggc ctgcggccct tcactcctgc acccctaccg
      841 gggagcgtgg ggtcgttcca ggcgcgtcaa gggccgctgc gctctccggg ggtggcccgt
      901 gtcctcgccc tgcgggctgc gggccgcgcc agccaccccc tgcgcccttg acccgccttc
      961 cactccggcg ctgtcggaaa tttcctaccc ggccatgagg cgatttccga cacgccagcg
     1021 ggccactggc tgacgccttc agcggagggc agggtcaagg gcgcgcccgc aggccgcagc
     1081 ccggagcgaa gcgcagggac cgggcgagga cgcgggcgaa gccgaaccct tgacgctgac
     1141 cgcagcgccc cagcctgtgc gagtgggggc agggacgaag ggcgaagccc gccacccctg
     1201 ccccccgagc ctcccggcgg cgagggtggg gagtccagag gggcaaagcc cctttgggca
     1261 aggccgaagg ccgccgcagg agcaagcccg aagggcgctg caaccgccgc aggcgggcac
     1321 gactttgctt cgcaaagtct agtgagtaca ctatcatctg atagtggccc gccggaggca
     1381 ccgccttgcg ctgccccgac tggccggatt cggacaccaa ggggaggggc aggcatggcg
     1441 gcctacgcga tcatgcgatg caagaagctg gcgacgatgg gcagcgtggc ggcggcgttg
     1501 cagcactgct accgcgagcg cgaaacgccc aacgctgacg ccgaacgcac gccggacaac
     1561 gagcaccggg cggccacctc aaccgatgcg gcgatggggc ggctgcgcga gttgctgccg
     1621 gagaagcggc gcaaggacgc ggtgctggcg gtcgaatacg tgatgacggc cagcccggaa
     1681 tggtgggcca aggccacgcc ccagcagcag gccgcgttct tcgaccaggc gcagggctgg
     1741 ctggcggcca aatacggcgc ggatcgcatc gtgacggcca gcatccaccg cgacgaggcc
     1801 accccgcacc tgtcggcctt cgtcgtgccg ctgacgcagg acgggcggct gtcggccaag
     1861 gagttcatcg gtggccgcga caagatgcgg gccgaccaga ccagctttgc cgaggcggtg
     1921 agggatctcg ggctggaacg cggcatcgag ggcagccggg ccacccatca gcgcgtccag
     1981 agctactacg gcgccatcga gcgccagccc gggcacgcca ccatcacgcc gcaggccatc
     2041 gagccgcggg tgctgcgcaa ggggattttc tcgaaggacg tggagacgcc ggaggccgtg
     2101 gccgcccgcg tgaccgctgc cgtcagggaa gggtatggcc cgaccgtcgc agcggccgca
     2161 ggagcgcgtc aggagcgcga gaaggcccgg caggcacagg agacagcccg gagcctgcgt
     2221 gaccgcctga agcccgttct ggacgctctg gggccgctta atcgcgatat gcaggccaag
     2281 gcggcccaga tcatcaaggc cgtgggcgag aagctgctcg ccgaacagcg cgaggcgaca
     2341 cgccagcggc aggcccaacg gcttcgcagt cgcggccggg atcgcgacga cggcgggctg
     2401 tctcgttgac cctacaggtg gccgacggaa ggggtttatg gcttgcagct actgaggacg
     2461 gtttgctcct tcccgccatc gtgggtcgaa aggttcatgt cgttgccctt accccacagt
     2521 tcgtaggtgt agtttttgct agcggagatg tagcgcactc ctgatgcgct cttggcgcgt
     2581 ttcagtggga caagctcatc catttgcagc atcacggcat agtctccgac gtacacgaca
     2641 tccaacgtct ttccgccttc gcaagcgtac ttggtctgga tcatctcatc cgctgctgaa
     2701 gcaagattcg gagcgattgc cgccatcatg gcagcaagag cgaacgtttt tttcataaag
     2761 attttctcct tcgtttcgtt gctcattggg gagtgctttc aaacatgtca atggtgttcg
     2821 ggtcgcggtc gtcgttctgc ttctcgccgc tgaacatcga catgatccgg cggcggatat
     2881 cgccatcctt gttgaccaag gttttggtca cgaaagctgc gtaatccttg gcgttgtcgt
     2941 agcccttcca gac
//
