LOCUS       NZ_CP078010            24350 bp    DNA     circular CON 13-OCT-2023
DEFINITION  Pseudomonas aeruginosa strain FAHZU31 plasmid pFAHZU31-KPC, complete
            sequence.
ACCESSION   NZ_CP078010
VERSION     NZ_CP078010.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19950563
            Assembly: GCF_019857645.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 24350)
  AUTHORS   Zhu,Y., Chen,J., Shen,H., Chen,Z., Yang,Q.-w., Zhu,J., Li,X.,
            Yang,Q., Zhao,F., Ji,J., Cai,H., Li,Y., Zhang,L., Leptihn,S., Hua,X.
            and Yu,Y.
  TITLE     Deciphering plasmidome of KPC-producing Pseudomonas aeruginosa
            reveals the blaKPC-2 copy number correlates with
            ceftazidime-avibactam resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24350)
  AUTHORS   Zhu,Y., Chen,J., Shen,H., Chen,Z., Yang,Q.-w., Zhu,J., Li,X.,
            Yang,Q., Zhao,F., Ji,J., Cai,H., Li,Y., Zhang,L., Leptihn,S., Hua,X.
            and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, Zhejiang University, 3 East Qingchun Road, Hangzhou,
            Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/13/2023 05:46:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,286
            CDSs (total)                      :: 6,207
            Genes (coding)                    :: 6,141
            CDSs (with protein)               :: 6,141
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 43 of 66
            Pseudo Genes (internal stop)      :: 6 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP078010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..24350
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="FAHZU31"
                     /host="Homo sapiens"
                     /db_xref="taxon:287"
                     /plasmid="pFAHZU31-KPC"
                     /geo_loc_name="China:Hangzhou"
                     /lat_lon="30.28 N 120.15 E"
                     /collection_date="2017-12-10"
     gene            1..1344
                     /locus_tag="KUU74_RS31130"
                     /old_locus_tag="KUU74_31125"
     CDS             1..1344
                     /locus_tag="KUU74_RS31130"
                     /old_locus_tag="KUU74_31125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049854848.1"
                     /translation="MGQAQLRLFSADDEAATYHDTARQGFFSLLMATGEGSGKKQDSYR
                     LSQMPVVLSMLDHSRDTWLSQAEFIKPNRRVVNLARIGLLFADLDTYREPWAQGRSPEQ
                     LAAAVMFRCYDEGVPPPSILVFSGRGVQAKWLLDGTLPRQALPRWNACQRYLIDRLAGL
                     GADPAAKDASRVLRLVNTVNSKSGEVCRVIHVEQGPDGEPIRYNFEYLAEALLPVARWD
                     IEADRKARADRRQFKLLPGGQTGNLRSLNGRQLAWDRLEDLRTLAALRGGVAEGERMQH
                     LFWRLNFLLLSGATHTGQMYHEAAALARELDPRWNYRSAELMTLYAKAKAHEAGEKVEF
                     GGKQFAPLYTPKNDTLISLFHITDDEQRKLRTLISRDMAAERHSEREKARRRAAGAVDR
                     ATYEANSASRQKPWEALGMSRRSWYRAGKPAAPADGTSPCVLQAEAGK"
                     /name="hypothetical protein"
     gene            1559..1861
                     /locus_tag="KUU74_RS31135"
                     /old_locus_tag="KUU74_31130"
     CDS             1559..1861
                     /locus_tag="KUU74_RS31135"
                     /old_locus_tag="KUU74_31130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072093556.1"
                     /translation="MSRARRSFPPALLDSLRAMTVQETLDRLGLYWKRDPDFVPVKDKA
                     TVRLNVSIGGGGVELLATGPKWYDTRAEQGGGGAIDLTMHLFRLSFVDAVKRLSP"
                     /name="hypothetical protein"
     gene            2202..2417
                     /locus_tag="KUU74_RS31140"
                     /old_locus_tag="KUU74_31135"
     CDS             2202..2417
                     /locus_tag="KUU74_RS31140"
                     /old_locus_tag="KUU74_31135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_261995155.1"
                     /translation="MSRIDALQQQALAQGLRERLTIRKRKVEAALARLDSGTYGLCCAC
                     HSDLEPELLNADPAVVFCQECATARQ"
                     /name="hypothetical protein"
     gene            2655..3530
                     /locus_tag="KUU74_RS31145"
                     /old_locus_tag="KUU74_31140"
     CDS             2655..3530
                     /locus_tag="KUU74_RS31145"
                     /old_locus_tag="KUU74_31140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="WP_222832122.1"
                     /translation="MARQGITFEQVAAVADALAGDGQQPTIRAVRERLGDTGSPNTIHK
                     HLAAWREARPVAAAAALELPQALTAAIAAEIERAASRARGEIEGRLVQAQAEAAELAAA
                     GEVLEGERDELAEQVAVLTTERDTLAGKAAQQAADLAEAQQRIEREQQAAESARLEVAT
                     ARHKIEAQAERVSEQAAEIERLRAALAAEQQGRTAAEQQAAVLAAKLEACADRVSRAEA
                     RADQIEQQAATAAQAHDTARAAAVQETRQAQAERDEARKVAAEAREQAARLAGQLEALT
                     AKERTSDERP"
                     /name="DNA-binding protein"
     gene            3517..4146
                     /locus_tag="KUU74_RS31555"
     CDS             3517..4146
                     /locus_tag="KUU74_RS31555"
                     /inference="COORDINATES: protein motif:HMM:NF037437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_065313977.1"
                     /translation="MKDRDHNEAMAGMFQAEPRFAADYLRQVLADGEPADVRAGLRQMA
                     DVLRVSQAAPPTDSAPSAGLFDRAGVRYEVACDVIGALIAHYAEIMGREREQAQPNEAV
                     LRVAGAMKAALAGERDDLDPRDSAGIEAAILRYAPLARRLYGPDENDHARQEQRRVDFD
                     QVNASLALEGLAMSADDLAVQALLIRGDITHDEAVQCYRILHRHAQ"
                     /name="antitoxin VbhA family protein"
     gene            4136..4420
                     /locus_tag="KUU74_RS31155"
                     /old_locus_tag="KUU74_31150"
     CDS             4136..4420
                     /locus_tag="KUU74_RS31155"
                     /old_locus_tag="KUU74_31150"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049854850.1"
                     /translation="MRSKTDWPPEVVGVLDLLESQPPEAAMLVGCFLAAVAHPDHVAEL
                     AMFDKLPSAARMVVGRFFSFFLAGGLDDAGREKLHSHMQAWFVRQRRSR"
                     /name="hypothetical protein"
     gene            complement(4673..4924)
                     /locus_tag="KUU74_RS31160"
                     /old_locus_tag="KUU74_31155"
     CDS             complement(4673..4924)
                     /locus_tag="KUU74_RS31160"
                     /old_locus_tag="KUU74_31155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348419.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_222832123.1"
                     /translation="MSPAAQSLAQTAHSARAVLAANMIRLRRERGWSQEALAFECGLHR
                     TFVAHCERQVRNISLDNLERIANAFGVSVYALLMPTGG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            5047..5388
                     /locus_tag="KUU74_RS31165"
                     /old_locus_tag="KUU74_31160"
     CDS             5047..5388
                     /locus_tag="KUU74_RS31165"
                     /old_locus_tag="KUU74_31160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223915932.1"
                     /translation="MLASLLIGAIEPAGAASLVGGQTDDSVCDLGSASQNARKIYEAGD
                     FIRAQCKNGQLLVGSGIVPAGGFDSEVVRLARTFCRMADIQTRRTQGNMAGLVMEIDEV
                     RCTIGKLPT"
                     /name="hypothetical protein"
     gene            5385..5639
                     /locus_tag="KUU74_RS31170"
                     /old_locus_tag="KUU74_31165"
     CDS             5385..5639
                     /locus_tag="KUU74_RS31170"
                     /old_locus_tag="KUU74_31165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_222832126.1"
                     /translation="MRKALLAGLLAVVAANVVAADGGAPLRGGVCIGPFRGADSVLHCE
                     HIGKVTIRQIYEKGFRVVHMQDDKNTASYVVLVIEEQAR"
                     /name="hypothetical protein"
     gene            5636..5938
                     /locus_tag="KUU74_RS31175"
                     /old_locus_tag="KUU74_31170"
     CDS             5636..5938
                     /locus_tag="KUU74_RS31175"
                     /old_locus_tag="KUU74_31170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223118780.1"
                     /translation="MRAKAWRMLFAGRRWMLWLPVPASVWLALPEWQHIPAMLFGGLIV
                     WIPFWLAWWLSDGFAGMSRWPGTGAPSVSGGVNPHTGKPCTVYHHPWGDTYVGGD"
                     /name="hypothetical protein"
     gene            complement(6020..>6154)
                     /locus_tag="KUU74_RS31635"
                     /pseudo=""
     CDS             complement(6020..>6154)
                     /locus_tag="KUU74_RS31635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008017239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /name="GDCCVxC domain-containing (seleno)protein"
     gene            6205..6969
                     /locus_tag="KUU74_RS31180"
                     /old_locus_tag="KUU74_31175"
     CDS             6205..6969
                     /locus_tag="KUU74_RS31180"
                     /old_locus_tag="KUU74_31175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="WP_001389365.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDHT
                     TIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTIDFY
                     LSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETAHRQ
                     VKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQPGIR
                     GEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
                     /name="IS6-like element IS6100 family transposase"
     gene            7334..8131
                     /locus_tag="KUU74_RS31185"
                     /old_locus_tag="KUU74_31180"
     CDS             7334..8131
                     /locus_tag="KUU74_RS31185"
                     /old_locus_tag="KUU74_31180"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016787 - hydrolase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="WP_223718344.1"
                     /translation="MAEVSAYLSSDADNLPRLSHEVAFDQEAAALSATAIAALLEGGAN
                     AELITERLLRNLCAHLRGRKGEKAPSKAHLELVLALEQLFGGQALRRAPHKRVLAAAQL
                     IAVQRLELQLTGDPQVDWVAARQFLDSSTEQVFKQIGIDGRYVRLLGKGSLLRSRLSEM
                     WRSAEGYSGAEKLVRDALIQDHFQAVTRDWKGLHLMTMHKSKGKEFTEVILYEGVMKGR
                     FLPTEATPDRVRQARLAMRVAVTRAMKKATILTPTGRNRCPLI"
                     /name="3'-5' exonuclease"
     gene            complement(8434..8751)
                     /locus_tag="KUU74_RS31190"
                     /old_locus_tag="KUU74_31185"
     CDS             complement(8434..8751)
                     /locus_tag="KUU74_RS31190"
                     /old_locus_tag="KUU74_31185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015270427.1"
                     /translation="MSNDAYLQTGQQPNYHLCYRTILCAMDERYDIRGYVLAEMVKTCL
                     KHRACLSAAQRECFMRYAQPEAIIYLEYLTANLLFGPQGRFSPKEYHYSQTADINQFRG
                     L"
                     /name="hypothetical protein"
     gene            complement(8748..9149)
                     /locus_tag="KUU74_RS31195"
                     /old_locus_tag="KUU74_31190"
     CDS             complement(8748..9149)
                     /locus_tag="KUU74_RS31195"
                     /old_locus_tag="KUU74_31190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015270426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015270426.1"
                     /translation="MNQEAFQISALSAAELADYLRNTKGTTSPGERYARAFGLNPAEFD
                     VYLATYRKSQADGATTTDSEAALRFIADVLRVVLMVTETGATIEQAIGWFRQDQLLTFE
                     GRTAEQLVSQGQVEQVLQFLASWQAGSQG"
                     /name="hypothetical protein"
     gene            9495..10118
                     /locus_tag="KUU74_RS31200"
                     /old_locus_tag="KUU74_31195"
     CDS             9495..10118
                     /locus_tag="KUU74_RS31200"
                     /old_locus_tag="KUU74_31195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172735239.1"
                     /translation="MNSANIELGRLAQELTDTQRNLEVEKRSTSKLTHDLQAERDAHEH
                     YLAALNEQRRQEQATFDNALTSLREDNRTLSADNIQLRGEILSLNKQLSGMTSQLTDLQ
                     AQNRKLALEADSAKDKLQVAEQSLMNLRIELATVKTQHSEAERQLESAVKERRETEEKL
                     VEAKASARTQNSEVRRLSAQLEGMKAELSSRVQSAEERGAEGNT"
                     /name="hypothetical protein"
     gene            10252..11406
                     /locus_tag="KUU74_RS31205"
                     /old_locus_tag="KUU74_31200"
     CDS             10252..11406
                     /locus_tag="KUU74_RS31205"
                     /old_locus_tag="KUU74_31200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_222832127.1"
                     /translation="MQLRAAASGLLLALLAGQTVAASFDCSKASSFPEKEICRDGYLSG
                     LDDWLAKSYRTALEVNPDQYEAVRQSQREWLATRDACTDQKCLDKTIGARIQALDQYVT
                     AERGKSASALAQAQLIKRQEEEAIRQRQTEERLAAEDAARQKQIENQRQQAIIELQQRA
                     QAAQAIESQRAHQQAPAQIPQYQPPQQVAQASSTTSFSPSYRAPEKSLWQQFKSSPAWK
                     YMLLAGALLTAWAMWRHHQGQATIYNDYTDAAITNLLPAAGVVLAFLMNWLELPRQLAM
                     VAGLTGFMLALMFAVYASVRTNQGWLSISLSLIAKMTLVSVFYAVLGALVASLFVSTKY
                     KGESQARANARNRREKKGLCCKDWRQSEVGCRSAPIRRLLRNGG"
                     /name="hypothetical protein"
     gene            complement(11378..12142)
                     /locus_tag="KUU74_RS31210"
                     /old_locus_tag="KUU74_31205"
     CDS             complement(11378..12142)
                     /locus_tag="KUU74_RS31210"
                     /old_locus_tag="KUU74_31205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="WP_001389365.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDHT
                     TIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTIDFY
                     LSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETAHRQ
                     VKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQPGIR
                     GEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
                     /name="IS6-like element IS6100 family transposase"
     gene            <12205..12360
                     /locus_tag="KUU74_RS31215"
                     /old_locus_tag="KUU74_31210"
                     /pseudo=""
     CDS             <12205..12360
                     /locus_tag="KUU74_RS31215"
                     /old_locus_tag="KUU74_31210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            12483..13463
                     /locus_tag="KUU74_RS31220"
                     /old_locus_tag="KUU74_31215"
     CDS             12483..13463
                     /locus_tag="KUU74_RS31220"
                     /old_locus_tag="KUU74_31215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213985.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481-like element ISKpn27 family transposase"
                     /protein_id="WP_013213985.1"
                     /translation="MTQALHSQARTTHLIREEIRNSTLPQAELARMYNVTRQTIRKWQN
                     RESPEDKSHAPNKMYTTLTPEQELIVVELRKTLLLPTDDLLAVTREFINPAVSRAGLGR
                     CLRRHGVSDLRNLVEQEGTAPATKKTFKDYEPGFVHIDIKYLPQMPDETARRYLFVAID
                     RATRWVFIELYADQTDGSSGDFLNKVQQACPVKIVKLLTDNGSQFTDRFTAGGKKKEPS
                     GTHVFDRLCKQLGIEHRLIPPRHPQTNGMVERFNGRISDIVNQTRFGSAAELESTLRNY
                     VKIYNHSIPQRALQHKTPVQALKEWHEKRPELFRKRVYNQPGLDI"
                     /name="IS481-like element ISKpn27 family transposase"
     gene            13739..14620
                     /locus_tag="KUU74_RS31225"
                     /old_locus_tag="KUU74_31220"
     CDS             13739..14620
                     /locus_tag="KUU74_RS31225"
                     /old_locus_tag="KUU74_31220"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /protein_id="WP_004199234.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-2"
     gene            complement(14870..>15751)
                     /locus_tag="KUU74_RS31230"
                     /old_locus_tag="KUU74_31225"
                     /pseudo=""
     CDS             complement(14870..>15751)
                     /locus_tag="KUU74_RS31230"
                     /old_locus_tag="KUU74_31225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            complement(15855..16151)
                     /locus_tag="KUU74_RS31235"
                     /old_locus_tag="KUU74_31230"
     CDS             complement(15855..16151)
                     /locus_tag="KUU74_RS31235"
                     /old_locus_tag="KUU74_31230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="WP_013213987.1"
                     /translation="MIRPETLRPFAEDWQAPTADEIKEVLELIRQRKGLSKPLSGVDVA
                     DLVGLPGERGSGKGTRTFRRWVSKTNPSPIAYGAWSILAHLAGFGAIWDADRD"
                     /name="transcriptional regulator"
     gene            complement(16197..16352)
                     /locus_tag="KUU74_RS31240"
                     /old_locus_tag="KUU74_31235"
     CDS             complement(16197..16352)
                     /locus_tag="KUU74_RS31240"
                     /old_locus_tag="KUU74_31235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_013213988.1"
                     /translation="MNTDIVSLAKHIGDALTARRPVHMPRMKMRELGLLLIELKAYREA
                     AATVTH"
                     /name="hypothetical protein"
     gene            complement(16480..16905)
                     /locus_tag="KUU74_RS31245"
                     /old_locus_tag="KUU74_31240"
     CDS             complement(16480..16905)
                     /locus_tag="KUU74_RS31245"
                     /old_locus_tag="KUU74_31240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="WP_013213989.1"
                     /translation="MMQTELNPLICSLVATPRRMAAMPRYVGRFYVVFESMLYQQMKGL
                     CREYRGAYWLMWELSNGGFYMAPGRRDEMLNIEAMNYFSGQMSADAAGITACLYLYSHL
                     SFHTEGADQERFSRLYHSLRDWACEHDEKEAILAAID"
                     /name="antirestriction protein"
     gene            complement(17016..>17120)
                     /locus_tag="KUU74_RS31560"
                     /pseudo=""
     CDS             complement(17016..>17120)
                     /locus_tag="KUU74_RS31560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            17178..17942
                     /locus_tag="KUU74_RS31250"
                     /old_locus_tag="KUU74_31245"
     CDS             17178..17942
                     /locus_tag="KUU74_RS31250"
                     /old_locus_tag="KUU74_31245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="WP_001389365.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDHT
                     TIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTIDFY
                     LSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETAHRQ
                     VKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQPGIR
                     GEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
                     /name="IS6-like element IS6100 family transposase"
     gene            <18011..18304
                     /locus_tag="KUU74_RS31255"
                     /old_locus_tag="KUU74_31250"
                     /pseudo=""
     CDS             <18011..18304
                     /locus_tag="KUU74_RS31255"
                     /old_locus_tag="KUU74_31250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939714.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /name="MerR family DNA-binding protein"
     gene            complement(18387..18962)
                     /locus_tag="KUU74_RS31260"
                     /old_locus_tag="KUU74_31255"
     CDS             complement(18387..18962)
                     /locus_tag="KUU74_RS31260"
                     /old_locus_tag="KUU74_31255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159185.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001173919.1"
                     /translation="MALIGYARVSTAEQDTALQTDALRNAGCERVFEDTASGAKADRPG
                     LADALAYLRDGDVLVVWRLDRLGRSLPHLIETVGKLEARGVGFRSLTENIDTTTPGGRL
                     IFHVFGALGQFERDLIRERTKAGLTAAAARGRKGGRKPVVTADKLQRARELVANGLNVR
                     EAAARLKVGKTALYAALQAASSGSAADS"
                     /name="recombinase family protein"
     gene            complement(19089..19295)
                     /locus_tag="KUU74_RS31265"
                     /old_locus_tag="KUU74_31260"
     CDS             complement(19089..19295)
                     /locus_tag="KUU74_RS31265"
                     /old_locus_tag="KUU74_31260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="WP_222935264.1"
                     /translation="MNDDLKPVLVALATDQPLDVRYRDHDLSGDWAGYRECHIKPDLLL
                     IYRKSDADTLRLARLGSHSELFG"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            19447..20016
                     /locus_tag="KUU74_RS31270"
                     /old_locus_tag="KUU74_31265"
     CDS             19447..20016
                     /locus_tag="KUU74_RS31270"
                     /old_locus_tag="KUU74_31265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003380658.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_043496681.1"
                     /translation="MNQRIGYARVSTDDQHLDLQRDALQQAGCSVIYEEAASGKSAARP
                     ELEQCRKALRAGDTLVVWRLDRLGRSLHDLVQIVAELEQRGVHFESLTEKIETGSASGK
                     LQFHVFAALAEFERGLIRERTQAGLAAARARGRAGGRKPKLDDQQVREIKALLRDPDIK
                     VAEVARRYGVSRTTLYKHVGVITPRQ"
                     /name="recombinase family protein"
     gene            20026..21495
                     /locus_tag="KUU74_RS31275"
                     /old_locus_tag="KUU74_31270"
     CDS             20026..21495
                     /locus_tag="KUU74_RS31275"
                     /old_locus_tag="KUU74_31270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686642.1"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]; GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="WP_049854844.1"
                     /translation="MLQQLDTADSVRREVAPRTAQKHKAEFGQFMTPSSVARFMASLFP
                     PSTLQTCRLLDAGAGVGALSCAFLDRWVTGGFGFESVEATAYEIDEKLRGHLAQHLAGY
                     SRVTPRIIAGDYIELATAEGLQDRGYTHAILNPPYKKINSQSDHRLALRTVGIETVNLY
                     SAFVALAVGEAAPGGQIVAIIPRSFCNGPYYRPFRDFILERAAIRHMHLFESRNKAFRD
                     DEVLQENIIIRLERGGQQGPVTVTTSTDDSFSDLTTHEHPFDRIVFPDDPERFIHVPTT
                     TEKSAIELSPAVRYSLADIGVKVSTGPVVDFRLKAHLRDMPEPGTVPLIYPGHLSITGT
                     VWPVPGLKKPNAIMRNDETEKWLYPNGFYCVVRRFSSKEEKRRVVASVVDPAAFGDHSV
                     LGFENHMNLFHEDKHGLPEALARGLAVFLNTTAVDEHFRRFNGHTQVNATDLKLMKYPS
                     RDTLTELGKWAMQQGTLTQEQIDAKLGTLTA"
                     /name="Eco57I restriction-modification methylase
                     domain-containing protein"
     gene            21492..22445
                     /locus_tag="KUU74_RS31280"
                     /old_locus_tag="KUU74_31275"
     CDS             21492..22445
                     /locus_tag="KUU74_RS31280"
                     /old_locus_tag="KUU74_31275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064713.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000287 - magnesium ion binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="WP_049854845.1"
                     /translation="MNNQNDYIEAAQQIIVSLGLPRAQQNERSALCLLALLNLTPGKAW
                     ADAENPLVGITPIMDWAREHYGKEYAPNTRETFRRQSMHQFCDAGVALYNPDKPDRPVN
                     SPKAVYQIEPAALALLRTFGTPAWHDSLAAYLAERETLVARYAKEREQNRIPVEIAPGQ
                     QITLSPGEHSELIRAIIEDFAPRFAPGSVLVYAGDTGDKWGYFDAALLAGLGVDVDSHG
                     KMPDVVLHFTAKNWLLLVESVTSHGPVDGKRHAELAKLFAGSTAGLVYVTAFPNRAIMG
                     RYLGEIAWETEVWVADAPSHLIHFNGVRFLGPYPTE"
                     /name="BsuBI/PstI family type II restriction endonuclease"
     gene            22463..22711
                     /locus_tag="KUU74_RS31285"
                     /old_locus_tag="KUU74_31280"
     CDS             22463..22711
                     /locus_tag="KUU74_RS31285"
                     /old_locus_tag="KUU74_31280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049854846.1"
                     /translation="MTTPTTETAKEVLRGYGRANEAALASLMDVQDWQRIARQELERRA
                     TSIVQALDDATLEAIAAGTLDMRAMCRQVADEIHQTA"
                     /name="hypothetical protein"
     gene            22866..23504
                     /gene="parA"
                     /locus_tag="KUU74_RS31290"
                     /old_locus_tag="KUU74_31285"
     CDS             22866..23504
                     /gene="parA"
                     /locus_tag="KUU74_RS31290"
                     /old_locus_tag="KUU74_31285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="WP_008166580.1"
                     /translation="MQVIAVLNQKGGAGKTTIATHLARALQLDGADVLLVDSDPQGSAR
                     DWAAVREDQPVPVVGIDRPTIERDLKSVARKDFVVIDGAPQAHDLAVSAMKAADCVLIP
                     VQPSPYDIWATSDLVDLVKQRIELTDGKLKAAFVVSRAIKGTKIGAEVSEALAGYGLPI
                     LQTRITQRVIYPSSAASGTTAIDQEPTSEAAEEIRALAEEVRRFLNCTI"
                     /name="parA"
     gene            23547..23747
                     /locus_tag="KUU74_RS31295"
                     /old_locus_tag="KUU74_31290"
     CDS             23547..23747
                     /locus_tag="KUU74_RS31295"
                     /old_locus_tag="KUU74_31290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partition protein ParG"
                     /protein_id="WP_049854854.1"
                     /translation="MKAGRPSAAKAGPTLSDLADKAATVRVNFDLDRAEHTKLKIYAAQ
                     TGRSITDILRELVRSLDEKSD"
                     /name="plasmid partition protein ParG"
     gene            23830..24183
                     /gene="parC"
                     /locus_tag="KUU74_RS31300"
                     /old_locus_tag="KUU74_31295"
     CDS             23830..24183
                     /gene="parC"
                     /locus_tag="KUU74_RS31300"
                     /old_locus_tag="KUU74_31295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020750543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParC family partition-associated protein"
                     /protein_id="WP_261995154.1"
                     /translation="MNNVDEKPGVRYKAMVESIEPLKLQVLPPAVFKEFAAATPHEIHG
                     AAVRNTEQGIVIVLNIGNERRILGQYRGGIRFFRSFDGAAAVLRQHGVLQWTADATGWI
                     PRTLEAKERSSTG"
                     /name="parC"
     ARG             13740..14617
                     /name="blaKPC-2"
                     /gene="blaKPC-2"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..1280
                     /name="IncP"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.286"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 atggggcagg cacagctacg acttttcagc gccgatgatg aagcggcgac atatcacgac
       61 acagcccggc agggcttttt ctcgttgctc atggcgacgg gcgagggcag cggcaagaag
      121 caggacagtt accggctgtc acagatgccc gtggtgctct ccatgctcga ccacagccgg
      181 gatacctggc tgtctcaggc cgagttcatc aagccaaacc gccgtgtcgt caatcttgcc
      241 cgtatcgggc tgctgttcgc tgacttggac acctaccgcg agccgtgggc gcaggggcgc
      301 agccccgagc aactggccgc cgctgtcatg ttccgctgct acgacgaagg tgtgccgccg
      361 ccgtcgatcc tcgttttctc tggccgtggc gtgcaagcaa agtggctgct ggatggcacc
      421 ttgccgcgtc aggcgctgcc gcgctggaat gcctgccagc gttacctgat tgaccgtctg
      481 gccgggctgg gcgctgatcc agcggccaag gatgcaagcc gcgtgctgcg gctggtcaat
      541 acggtgaaca gcaagagcgg cgaggtttgc cgcgtcatcc acgttgagca aggcccggac
      601 ggcgagccga tccggtacaa cttcgagtat ctggcggagg cgctgctgcc ggtggcccgc
      661 tgggacatcg aggccgaccg caaggcccgc gccgaccgtc gccagttcaa gctgctgccg
      721 ggcggccaaa ccggcaacct gcgcagcctc aacgggcggc aactggcgtg ggatcgtctg
      781 gaggacttgc gcacgctggc cgcgctgcgc ggcggcgtgg ccgaaggcga gcggatgcag
      841 cacctgtttt ggcggctgaa cttcctgctg ctgtcaggcg cgacgcacac gggccagatg
      901 taccacgagg ccgccgcgct ggcccgtgag cttgatccac ggtggaacta ccgcagcgcc
      961 gagcttatga cgctgtacgc caaggccaag gctcacgaag ccggggaaaa ggtggagttc
     1021 ggcggcaagc agtttgcgcc gctttacacg cccaagaacg acacgctgat ttccctgttc
     1081 cacatcaccg acgacgagca acgcaagctg cgcacgctca ttagccggga catggccgca
     1141 gagcgccaca gcgagcgcga gaaggcccgc aggcgtgccg ctggggctgt cgatcgtgcg
     1201 acctatgagg ccaattccgc gagccgtcag aagccgtggg aagccttggg catgagccgc
     1261 cgcagttggt atcgggcagg gaaaccagcc gcgcctgccg atggcacaag tccgtgcgta
     1321 ttacaggcgg aggcggggaa atgagggttg gcacaagtcc gtgcgtatta ctaatggcga
     1381 agcctacgcc cggcgcgaag cgcctgtttt tgagtagccc agttttattt ataaaacgag
     1441 tagcccggca aggacggtgg gtaactgctt gcagttatcc acgggccgcg cagcgccgga
     1501 acggcgcgga gagccgcccg cagggcggcc ccctcgtcca gttcgccggg tggctgtcat
     1561 gagccgtgcc cgtcgatcct ttccgcctgc gctgctggat tcgctgcggg caatgacggt
     1621 gcaggaaacg ctagaccggc tgggcctgta ctggaagcgt gatcctgact ttgtgccggt
     1681 gaaggacaag gccacggtgc ggctgaatgt gtccattggt ggcggcggtg ttgagctact
     1741 ggcgaccggg ccgaagtggt acgacacacg ggcagagcag ggcggcggcg gtgccatcga
     1801 cctgaccatg cacctgttca gattgtcatt cgtggatgcg gtcaaacggt tgtcgccttg
     1861 atcgtgtttg caagatgcct ccggcggccc tgccgggggc caaacggtcg cgtttgatcc
     1921 tcgggagcag ggggacggcc tgcggcctaa acccctgccc cactcgggga gcgtgagccg
     1981 ttccgagggc atcaagcgac tttcgcggca taaacggtgc ccccctacgg gctacccccc
     2041 atttattcca cggttcgctt gaccccctct ccactcgaaa acccggacgg cggacgacct
     2101 cgcaggcggc ccgtggcggc gttttctcga cgtggccctg cctgccgcag gctcaacagt
     2161 ccgccgcgcc ggtcgagctg gatcaatcct gtgtcggtag ggtgtcgcgt atcgacgccc
     2221 tccaacaaca ggctcttgcc caaggattgc gggagcggct gaccattcgc aagcgcaagg
     2281 tcgaagctgc gctggcccgc ctcgactctg gcacctatgg gctgtgctgc gcctgtcaca
     2341 gtgatttgga gccggaactg ttgaacgccg atcctgctgt tgtgttctgc caggaatgcg
     2401 cgacagcgcg tcaatgacaa agccgtattg atgcccaacg gcggagccgt tttgccttaa
     2461 cgtgctttat tttccgtttt ctgaggcgac cccaacgagc cgaaggcgag gcgtcagcac
     2521 ggtgtttcac ccaaaaagag cagccgaccg ggcagcggta cgttacgaaa accgcttgca
     2581 tatcgtatct gtattgtgta tcatatctgt attgatacga tttacaccga tacggtacga
     2641 aaggagcaac gatcatggca cgtcagggca tcacttttga gcaggtcgcc gccgtcgctg
     2701 atgcgctggc aggcgatggt cagcagccca ccatccgggc agtgcgcgag cggttgggcg
     2761 acaccggcag cccgaacacc atccacaagc atctggccgc gtggcgtgag gctcgcccgg
     2821 tggccgccgc cgctgcgctg gaactgccgc aggcgctgac cgctgccatt gccgccgaaa
     2881 tcgagcgggc cgcatcgcgg gcacgcggcg agatcgaggg ccgtctggtg caggcgcagg
     2941 cggaggccgc cgagctggcc gccgctggcg aggtgctgga aggcgaacgc gacgagctgg
     3001 ccgagcaggt ggccgtgctg accactgagc gcgacacgct ggcaggcaaa gccgcccagc
     3061 aggccgccga cttggccgag gcgcagcagc gcatcgagcg cgagcagcag gcggcagagt
     3121 ctgcccggct ggaggtggca accgcccggc acaagatcga ggcgcaggcc gagcgcgtga
     3181 gcgagcaggc cgcagagatc gagcgcctac gcgctgcgct ggccgccgaa caacagggcc
     3241 gcaccgccgc cgaacaacag gccgccgtgc tggccgccaa gctggaggcg tgcgccgacc
     3301 gtgtgagccg tgcagaggct cgcgccgatc agatcgagca gcaggccgcc acggctgcgc
     3361 aggcccacga caccgcccgc gccgcagcag tgcaggaaac ccggcaggcg caggccgagc
     3421 gcgacgaagc ccgcaaggtg gccgccgagg cccgcgagca ggccgcgcgc ctggcggggc
     3481 aattggaagc cctcacagcg aaggagcgga catccgatga aagaccgtga ccacaacgaa
     3541 gcgatggccg gcatgtttca ggccgaaccc cgatttgccg ccgactatct gcggcaggtg
     3601 ttggcggatg gcgagcctgc ggacgtgcgc gccggcttgc ggcaaatggc ggatgtgctg
     3661 cgtgtcagcc aggccgcccc gccgaccgat tccgcgcctt cggcgggtct ctttgaccgg
     3721 gccggtgtgc gctacgaagt ggcgtgcgat gtgatcgggg cgttgattgc ccattacgcc
     3781 gaaatcatgg gacgggaacg cgagcaggcg cagccgaatg aggcggtttt gcgcgttgcc
     3841 ggggcgatga aggcggcgct ggccggggag cgggatgatc tcgatccgcg cgacagcgcc
     3901 ggcatcgagg cggcgatttt gcgctatgcg ccattggcgc gccggctgta tggcccggat
     3961 gaaaacgacc acgcccgcca ggagcagcgc cgcgtcgatt tcgaccaggt gaatgcttcg
     4021 ctggccttgg agggtctggc gatgagcgcg gatgatttgg cggttcaggc gctgctgatc
     4081 cggggcgata tcacccacga tgaggcggtg cagtgctacc gcatcctgca tcgccatgcg
     4141 cagtaaaacc gactggccac ccgaggtggt cggtgtactg gatttgcttg aatcgcagcc
     4201 gccagaggcg gcgatgctgg tgggctgttt tctggcggcg gtggcccatc ccgatcatgt
     4261 ggccgagctg gcgatgttcg acaaattgcc gtcagcggcc cggatggtcg tcggtcggtt
     4321 tttctcgttt ttcctggcgg gcggcctgga cgatgccggg cgcgaaaaac tgcattcgca
     4381 catgcaggca tggttcgtcc gtcagcgccg ttcccggtga atggcctgcc tcatccacta
     4441 gggccgggca agggatggac agcaggcgat gctggcttgc gggacggccc cgcaccccca
     4501 gaaaacttgt cacacaccac gcaactcgcg ttgcttcgct aaaagctttg tgccgcaagg
     4561 cttttagcgc ggcgacaccg aagacacatc gcggcgacac cgaacgggcc gaaccagctt
     4621 aaaacccttg ccgcgcaagg aaaaaagccg tgctttcgca cgcgaaagtg gttcaaccgc
     4681 ccgtcggcat cagcagggca tagacagaca cgccgaaggc attggcgatg cgctccaggt
     4741 tgtccaggga gatgtttctg acttggcgct cgcagtgcgc aacaaaggtg cgatgtaggc
     4801 cgcactcgaa ggcgagcgct tcttgtgacc agcctctttc ccggcgtaat cggatcatat
     4861 tggccgcgag cacggcccgc gcggaatgcg cggtttgtgc gagagattgg gcggcgggag
     4921 acatgcaaac cagtctcccg atgtgctgct tttacgtcag ccgtgtttaa gtcacaatat
     4981 ggtttcctca tagaagacaa cggcgtggcg atgggcagaa aaacagcaat caggcgaggg
     5041 tgtgccgtgt tggccagcct gttgattggc gcaattgaac cggccggcgc ggcctccctg
     5101 gtcggcggtc aaaccgacga ttccgtgtgc gacctgggca gcgcctcaca gaacgcccgc
     5161 aagatttacg aggcgggcga cttcatccgc gcgcagtgca aaaacggtca actgttggtg
     5221 ggttccggca tcgtgcctgc tggcggattt gactcggagg tggtgcgcct ggcgcgcacc
     5281 ttctgccgca tggcggacat tcagacccgg cgcacacagg gcaacatggc cggcctcgtc
     5341 atggagatcg acgaggtacg gtgcaccatc gggaagctgc cgacatgaga aaagcgctgc
     5401 tggctggcct cctggccgtt gtggcggcaa acgtggttgc cgctgatggc ggcgcgccct
     5461 tgcgcggcgg cgtttgcatc ggcccgttcc ggggcgcaga ttccgttctg cactgcgagc
     5521 acatcggcaa ggtgacgatc cgccagattt acgaaaaggg ctttcgggtc gttcacatgc
     5581 aggacgacaa gaacacagcc agctacgttg tgctggttat tgaggagcag gcgcgatgag
     5641 ggcgaaggcg tggcggatgc tgtttgccgg gcggcgctgg atgctctggc ttcccgtgcc
     5701 ggcgtcggta tggctggcgc tccccgaatg gcagcacatc cccgccatgt tgttcggcgg
     5761 cctgatcgtg tggattccct tctggctcgc gtggtggctc agtgatggtt tcgcgggcat
     5821 gtccaggtgg cccggaaccg gcgcgccttc ggtgtcgggc ggggtgaacc cgcacaccgg
     5881 caagccatgc acggtgtatc accatccgtg gggagatacc tacgtcggtg gggactgagt
     5941 gcccgcttga agaccgatga caagggccgg cttttgctgg cccttgtcgt tttgtaggtg
     6001 ggtggccttc tggatggagc tatccgcaac atccgcgctg ctgttggatt ggcgggcatg
     6061 gcaccgagcc gaacgaacag aacacgcagc aatcacccgg attgggacgc agcagtgcct
     6121 tgcagtttgg gcattcgtaa tagggctctg ttgcaaaaat cgtgaagctt gagcatgctt
     6181 ggcggagatt ggacggacgg aacgatgacg gatttcaagt ggcgccattt ccagggtgat
     6241 gtgatcctgt gggcggtgcg ctggtattgt cgctatccga tcagctatcg cgaccttgag
     6301 gaaatgctgg cggaacgcgg catttcggtc gaccatacga cgatctatcg ctgggtccag
     6361 tgctacgccc cggagatgga gaagcggctg cgctggttct ggcggcgtgg ctttgatccg
     6421 agctggcgcc tggatgaaac ctacgtcaag gtgcggggca agtggaccta cctgtaccgg
     6481 gcagtcgaca agcggggcga cacgatcgat ttctacctgt cgccgacccg cagcgccaag
     6541 gcagcgaagc ggttcctggg caaggccctg cgaggcctga agcactggga aaagcctgcc
     6601 acgctcaata ccgacaaagc gccgagctat ggtgcagcga tcaccgaatt gaagcgcgaa
     6661 ggaaagctgg accgggagac ggcccaccgg caggtgaagt atctcaataa cgtgatcgag
     6721 gccgatcacg gaaagctcaa gatactgatc aagccggtgc gcggtttcaa atcgatcccc
     6781 acggcctatg ccacgatcaa gggattcgaa gtcatgcgag ccctgcgcaa aggacaggct
     6841 cgcccctggt gcctgcagcc cggcatcagg ggcgaggtgc gccttgtgga gagagctttt
     6901 ggcattgggc cctcggcgct gacggaggcc atgggcatgc tcaaccacca tttcgcagca
     6961 gccgcctgat cggcgcagag cgacagccta cctctgactg ccgccaatct ttgcaacaga
     7021 gccaatctac gaattccgcg gtgccagtgc tagcaggatt caagagttcg aacaggccta
     7081 ccgccccgcc tccttttctt ttggaatgga aaatcaccgg agcacaggga ccgacattgt
     7141 ccagttcggc aacgatctgc tggcaggagt aaataggggc agagcctaca cccatgtgtc
     7201 ggttacaccc tatgcgccgc gcaaggggat gctccatcgg gctttcaaat ttgccattct
     7261 gactgctata gcacggttga atagatacgg acgtgattgg tcactagcca ttctggttcc
     7321 cagcaaaagc ttcatggctg aggtttctgc gtacctttcg agtgatgcgg acaatctgcc
     7381 caggctttcc catgaggtgg cgttcgacca ggaggcggca gcgctctctg ctacggcgat
     7441 cgccgcgttg ctggaaggtg gcgcgaatgc tgaactcatt actgaaagac ttcttagaaa
     7501 cctatgtgct catctgcgtg gcaggaaagg cgagaaagcc ccaagcaagg ctcacttgga
     7561 attggtactc gctctggagc agttgttcgg tgggcaagca cttcgtcgag cgccgcacaa
     7621 gcgggttctt gctgccgcac agcttattgc agtccagcgt cttgagttgc aattgactgg
     7681 cgacccccaa gttgactggg ttgccgcacg acaatttctg gattcaagca ccgagcaggt
     7741 tttcaagcag atcggtatag atggtcggta tgtgcgcttg cttgggaagg ggtcgctgct
     7801 tcgaagccgt ttgagcgaga tgtggcgctc agctgaaggc tattcaggag cagagaagct
     7861 ggtgcgggat gctcttattc aggaccactt tcaagctgtc accagggact ggaaggggct
     7921 ccatctgatg accatgcaca aatcgaaggg aaaggaattc acagaggtca ttctttatga
     7981 gggagtgatg aaggggcgtt tcttgccgac cgaagcaacc ccggatcgcg tgcgtcaggc
     8041 ccgtttagcc atgcgtgtcg ctgtcactcg tgcaatgaag aaggcgacta tcttgacgcc
     8101 gactgggcga aatcgctgcc cgttgatttg aacgcaagat tggggatgcc gccagggacc
     8161 atcgtattat tactgatgtg cggctgatcg gacgttgcga tccgctaacc tagagtgaaa
     8221 gacaaaatgc ccaggcccaa ccacctcgcc ttctttgtct ggcggataat ggtgagcggg
     8281 ttccagatta gtgcaaaacg gcgctggttg atcaaggttg taccattacc cctttcttac
     8341 agctcgcgga tgatactccg attaatcggc tttcgaataa gtacgagttt gatcctgtcc
     8401 gatgtcctgt acactagctc tttttcattt tcatcagagg cctcgaaact gattgatatc
     8461 tgctgtttgt gaataatgat actccttggg tgaaaagcgt ccctgtgggc caaacaataa
     8521 attggccgtt agatattcga gatagatgat cgcctccggt tgcgcatatc gcataaagca
     8581 ctcgcgctgg gccgcagata gacaggctcg gtgcttcaga catgttttca ccatttccgc
     8641 cagtacatag ccgcgtatgt cgtatcgctc atccatggcg caaaggatgg ttcgatagca
     8701 taagtgatag ttcggttgct ggccggtctg taggtaggcg tcgttactca tccttgactc
     8761 ccggcctgcc aggaggccag aaattgcagc acctgctcaa cttggccctg actgactagt
     8821 tgctccgcag ttcgcccctc gaaagttaac aattgatctt ggcggaacca gccaattgct
     8881 tgctcgatag tcgcaccggt ctcagtcacc atcagaacca cacgtaggac atctgctatg
     8941 aagcgtagcg ctgcttcgga gtcagtcgtg gttgcaccat ctgcctggct tttgcgatac
     9001 gtagcgaggt aaacgtcaaa ctcagctggg ttcagaccaa aagccctggc gtaacgttcc
     9061 cctggagagg ttgtcccttt cgtgttgcgt aggtaatcag ccaattcagc agctgacagc
     9121 gcgcttattt gaaatgcctc ttggttcatg acttgttccc ctcgcgacct ttcgttaaca
     9181 gcatgccccc aaatcaacag taagcaaagc catagctgtg tggcttttat ggtcgacgtg
     9241 gcaagggcgg tcgttcagcg gaagcgtatg tacgccacgg atgtggagct ttcacgcaac
     9301 ggcctaggag catcccccga tgacacagta gacacagaga gggccaggcg gaacgactga
     9361 gaccacgcga tggtggtggg cggcctggaa ctaatgttta gggaatgtgc tgtagggtcg
     9421 atttgggggt ttgacttacc gctttttggc cttttcagtt ctgattactg ctgctgggcg
     9481 aactgactga gcgcttgaac tcggccaaca ttgagttggg ccgcctggcc caggagctga
     9541 ctgacacgca acgtaacctg gaggtggaga agcgctcgac cagcaagcta acccacgacc
     9601 ttcaagcaga gcgggatgct catgagcact atctcgcagc cctgaacgag caacgccggc
     9661 aggagcaggc gactttcgat aacgcgctga cctctctgcg cgaagacaac cgcaccctct
     9721 cggcggacaa catccaactg agaggggaaa tcctgagcct gaataagcaa ctgtctggca
     9781 tgacctccca gttgaccgac ctgcaagcgc agaacaggaa gttggcgctt gaggctgaca
     9841 gcgcgaagga caagctgcag gtcgcagagc aatcactcat gaacctgagg atcgaactcg
     9901 ccacggtaaa aactcagcat tcggaggccg aaaggcagct tgaaagcgcc gtcaaggaga
     9961 gacgagagac cgaggagaaa ctggtggaag caaaagcctc tgctcgaact cagaacagcg
    10021 aggtgagacg cctttccgcc cagctggagg gcatgaaggc agagctttcc tcccgcgtcc
    10081 aatccgcgga agaacgtggt gcggaaggaa acacctgagg acctggcaga tagccatcag
    10141 taggactgac tggcatcatt cggctcgggc gttatggtca acgggggctc ataaaccgcc
    10201 gtgggccgcc gataacgcag acaggcgatc aacaagtaaa ggatgatctt gatgcaactt
    10261 cgtgcagcag cctccggtct gcttctggcc ctcctggccg gccaaactgt ggcagcaagc
    10321 tttgactgct ccaaggccag cagcttcccc gaaaaagaaa tctgccgtga tggctacctt
    10381 tccgggttgg acgattggtt agccaaatcc tataggaccg ctctggaggt aaatcctgac
    10441 cagtacgagg cggtccgtca gtcgcagcgt gaatggctcg ctacaaggga tgcatgtact
    10501 gaccaaaagt gcctcgacaa aaccatcggc gcccgcatac aggcgcttga tcagtatgtg
    10561 acggcagaga ggggtaaatc ggcatcggct ttagctcagg cccagctcat caagcgccag
    10621 gaagaggagg ccatcaggca gcggcaaact gaggagcgcc tggcggctga agacgcagct
    10681 cgacagaagc agatcgagaa tcagcgccag caggcgatca tcgaactcca gcagcgcgct
    10741 caggccgccc aagcgatcga atctcaacgg gcgcaccagc aggcaccagc ccaaatcccg
    10801 cagtaccaac caccccaaca ggttgcgcaa gcttcgagta caacctcgtt ctcgccgtca
    10861 taccgcgcgc cggagaaatc tctgtggcag cagttcaaat ccagcccggc ctggaagtac
    10921 atgctgctgg cgggtgcgct tctgacagct tgggcgatgt ggaggcacca tcaggggcaa
    10981 gcaaccatct acaacgacta tacggatgcc gcaatcacga acctcttgcc ggctgcaggc
    11041 gtggtgttgg ccttcttgat gaactggctc gagctccccc gtcaactcgc aatggtcgca
    11101 ggcctaacgg gtttcatgct tgcattgatg ttcgcggtgt atgcgtcagt gcgaaccaat
    11161 cagggatggc tgagcatcag cctgtcactc atcgccaaaa tgactctggt gtcagtgttc
    11221 tatgcggtgc tcggcgcact ggtggcatca ctattcgtaa gcacaaagta caaaggggag
    11281 tcccaggcta gagcgaatgc aaggaatcga agggagaaga aagggctctg ttgcaaagat
    11341 tggcggcagt cagaggtagg ctgtcgctct gcgccgatca ggcggctgct gcgaaatggt
    11401 ggttgagcat gcccatggcc tccgtcagcg ccgagggccc aatgccaaaa gctctctcca
    11461 caaggcgcac ctcgcccctg atgccgggct gcaggcacca ggggcgagcc tgtcctttgc
    11521 gcagggctcg catgacttcg aatcccttga tcgtggcata ggccgtgggg atcgatttga
    11581 aaccgcgcac cggcttgatc agtatcttga gctttccgtg atcggcctcg atcacgttat
    11641 tgagatactt cacctgccgg tgggccgtct cccggtccag ctttccttcg cgcttcaatt
    11701 cggtgatcgc tgcaccatag ctcggcgctt tgtcggtatt gagcgtggca ggcttttccc
    11761 agtgcttcag gcctcgcagg gccttgccca ggaaccgctt cgctgccttg gcgctgcggg
    11821 tcggcgacag gtagaaatcg atcgtgtcgc cccgcttgtc gactgcccgg tacaggtagg
    11881 tccacttgcc ccgcaccttg acgtaggttt catccaggcg ccagctcgga tcaaagccac
    11941 gccgccagaa ccagcgcagc cgcttctcca tctccggggc gtagcactgg acccagcgat
    12001 agatcgtcgt atggtcgacc gaaatgccgc gttccgccag catttcctca aggtcgcgat
    12061 agctgatcgg atagcgacaa taccagcgca ccgcccacag gatcacatca ccctggaaat
    12121 ggcgccactt gaaatccgtc atcgttccgt ccgtccaatc tccgccaagc atgctcaagc
    12181 ttcacgattt ttgcaacaga gccgctgaaa ggaatcaaat ttggccgcag gcgtaccgtg
    12241 gacaggaacg tcgtgctgac gcttcatcag aagggcactg gtgcaacgga aattgctcat
    12301 cagctcagta ttgcccgctc cacggtttat aaaattcttg aagacgaaag ggcctcgtga
    12361 tacgcctatt tttataggtg tctggactcg tgggatcatg tacccatgcg tagctggccg
    12421 ctcttcaagg ccagacacgt tttgcgtaca ctgttccaat ccgactcttc actggcaact
    12481 cgatgaccca ggcactgcac agccaagccc gtactaccca cctgatccgt gaggaaatca
    12541 ggaactcgac gctcccgcag gccgaactgg ccaggatgta caacgtcacc cgccaaacca
    12601 tccgaaagtg gcaaaaccgc gagtctcctg aagacaagtc gcatgcgccg aacaagatgt
    12661 acacgacgct cacgcccgag caggagctca tcgtggtgga gctgcgcaag acgttgctgc
    12721 tgcccacgga cgacctgctg gcggtcacgc gcgagttcat caatccagcc gtctcgcgtg
    12781 ccggcctggg acgttgcctg cgccgccacg gtgtctcgga tctacgtaac ctggtcgagc
    12841 aggaaggcac tgcgcccgcc acgaaaaaga ccttcaagga ctacgagccg ggctttgtgc
    12901 acatcgacat caagtacctg ccgcagatgc ccgacgagac ggcaaggcgc tatctcttcg
    12961 ttgccatcga ccgtgctacg cgctgggtct tcatcgagct ctatgccgac cagaccgatg
    13021 gcagcagtgg cgacttcctc aacaaagtcc agcaagcctg tcccgtcaag atcgtcaagc
    13081 ttctgaccga caacggcagc cagttcaccg accgcttcac ggctggcggc aagaagaagg
    13141 aacccagcgg cacacacgtg ttcgaccgcc tgtgcaagca gctcggcatc gagcaccggc
    13201 tcatcccgcc tcgtcatccg cagaccaacg gcatggtgga gcgcttcaac ggtcgtatca
    13261 gcgacatcgt caaccagacc cgttttggtt cagctgccga actggaatcg acgctgcgca
    13321 attacgtcaa gatctacaac cacagcattc cgcaacgcgc gctccaacac aaaacacccg
    13381 ttcaggcgct caaggaatgg catgaaaaac gccctgaatt gttcaggaaa cgcgtgtata
    13441 accagccggg tcttgacata taggttaatg tcatgataat aatggtttct tagacgtcag
    13501 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    13561 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    13621 tgacttctta acgtgagttt tcgttccact gagcgtcaga cccctaatcc cagctgtagc
    13681 ggcctgatta catccggccg ctacacctag ctccaccttc aaacaaggaa tatcgttgat
    13741 gtcactgtat cgccgtctag ttctgctgtc ttgtctctca tggccgctgg ctggcttttc
    13801 tgccaccgcg ctgaccaacc tcgtcgcgga accattcgct aaactcgaac aggactttgg
    13861 cggctccatc ggtgtgtacg cgatggatac cggctcaggc gcaactgtaa gttaccgcgc
    13921 tgaggagcgc ttcccactgt gcagctcatt caagggcttt cttgctgccg ctgtgctggc
    13981 tcgcagccag cagcaggccg gcttgctgga cacacccatc cgttacggca aaaatgcgct
    14041 ggttccgtgg tcacccatct cggaaaaata tctgacaaca ggcatgacgg tggcggagct
    14101 gtccgcggcc gccgtgcaat acagtgataa cgccgccgcc aatttgttgc tgaaggagtt
    14161 gggcggcccg gccgggctga cggccttcat gcgctctatc ggcgatacca cgttccgtct
    14221 ggaccgctgg gagctggagc tgaactccgc catcccaggc gatgcgcgcg atacctcatc
    14281 gccgcgcgcc gtgacggaaa gcttacaaaa actgacactg ggctctgcac tggctgcgcc
    14341 gcagcggcag cagtttgttg attggctaaa gggaaacacg accggcaacc accgcatccg
    14401 cgcggcggtg ccggcagact gggcagtcgg agacaaaacc ggaacctgcg gagtgtatgg
    14461 cacggcaaat gactatgccg tcgtctggcc cactgggcgc gcacctattg tgttggccgt
    14521 ctacacccgg gcgcctaaca aggatgacaa gcacagcgag gccgtcatcg ccgctgcggc
    14581 tagactcgcg ctcgagggat tgggcgtcaa cgggcagtaa ggctctgaaa atcatctatt
    14641 ggcccaccac cgccgccctt gcgggcggca tggattacca accactgtca catttaggct
    14701 aggagtctgc gcggcagagc cgtgtgaccg gttttctgta gagcactgac gatggcggcg
    14761 gcgctctctg caattggcaa ggcgtcggcg ccaaggatac caatcttgcg gcgcgcggcg
    14821 tgttatgacg actggggtgc atttgagccg ccccatttaa ccttcgccct cacagatacg
    14881 ccattcgcct cagatttagc gccatgcaga cgagcttcca ctcggcttgc accttgtcca
    14941 ggcccctcat gctgaactga cgcaatccca tcaccgcctt gatccaacca ttcggagcct
    15001 ccacgatcga cttgcgccgg cggtaagctg catcgccttg ctccgttttc aatttcgccg
    15061 caatcgccgc cgtatgcgga tgggtcttgg cattgacctt ggcatcttca cgtccctcgc
    15121 ggccgagggc aacgatgaca tcgccgtggt gatcggcgac ctttgccaga acagcctcac
    15181 tacggaatcc cgcatccgcc agcgtctggg ccggcatttc tccggtgttg gcctgaactg
    15241 ctgccagcat gcccagcagc gcctgactgt ccgcggcgca gttggtcaac tccgccgcca
    15301 cgatgatctg gtgctcggca tcgaccgctg tgtacccgtt gtagctctgc tcggagccac
    15361 caccggcgtg tttcatgatc cggctgtccg gatcggtgaa gctttcctga tcacggtcat
    15421 ccggcacacc aaactcgcgt ttgtacgagc caccgccctt gtccgagcca tccggatggc
    15481 gaggccggcg gccatcgtct tcgctgcgcc cccgggcctg gtccgcttca cgctggcgcg
    15541 cttccaggcg cgcctttgcc gcctggatcg cctccaggcg cttctcgcgg cgagaaatct
    15601 cggcaggaat gtccagctcc ggctcgttac gctcctggtc gtcggtagcc ttggcgcgat
    15661 caagcagcgc cttgatctcg caatgcaatt cgtcctcggc cggcttcatg cgcttatagc
    15721 tcatcgcctt gtggtcgcca tgtttggcgg cttcgtattc aggttcttta gagagatcct
    15781 ttagatcgcc gttcctcact actgccggga agtctcccca gggagcacgt tcggccttat
    15841 gttcggtcat ggtttcaatc ccggtcagcg tcccatatag ccccgaaacc tgccaggtgg
    15901 gccaagatag accaggcacc ataagcaata gggctagggt tggttttgct cacccaacgc
    15961 ctgaaggttc gggttccctt tccgctgcct ctctcaccag gcaaaccaac gagatcagcg
    16021 acatcaacgc cactgagagg cttactaagc ccttttcgtt gcctaatcag ctccaacacc
    16081 tctttgattt catcggcggt tggagcctgc caatcttcag cgaacggcct aagcgtttca
    16141 ggtctaatca tgcgataaat cctcaaatag ccccggtcaa tgccggggct tttttatcaa
    16201 tgggtaacgg tcgcggcggc ctcccgatac gccttcagtt caatcaagag caagccaagc
    16261 tcccgcatct tcatgcgcgg catatgaacg gggcggcggg ccgtcagagc gtcgccaatg
    16321 tgttttgcaa gtgatacaat atccgtattc atcgcgttta tcctcaaatt gaccggtgaa
    16381 agcccctgtg attggttgca gccataccag gggcaccatt tctttttcta cctatgcacc
    16441 tcctttaaga gccccggcca tcaccggggc tttctgttgc tagtctattg cggccaagat
    16501 ggcctctttt tcgtcatgct cacaagccca atcccgcaag ctgtgataca gccttgaaaa
    16561 ccgctcctgg tcagcgcctt ctgtgtgaaa tgacagatgg ctatagaggt agaggcaagc
    16621 ggtaatcccg gcagcatcgg cgctcatttg gccactaaag tagttcatgg cctcgatgtt
    16681 cagcatttca tcacgacgcc caggagccat ataaaagccg ccgttcgaca gctcccacat
    16741 aagccaataa gccccgcgat actcgcggca gagccctttc atttgctggt aaagcatcga
    16801 ctcgaaaacc acatagaaac ggcctacata gcggggcatt gcagccatac ggcgaggggt
    16861 ggcgaccagg gagcagatca ggggattaag ttctgtttgc atcatcggag ttctccggta
    16921 ttgaccgtgg cgggtgcccg gccttctgag atatataata ggccattttg gcctattgtg
    16981 tcaagcgggt acaataaaaa ataggccaaa tcggcctact ttttttctct ccaactgagc
    17041 cacattttat tcagctccag gctgcgggct ggccaatctg gcagattcac cgcatgggcg
    17101 cggtagttga tccgagggct ctgttgcaaa aatcgtgaag cttgagcatg cttggcggag
    17161 attggacgga cggaacgatg acggatttca agtggcgcca tttccagggt gatgtgatcc
    17221 tgtgggcggt gcgctggtat tgtcgctatc cgatcagcta tcgcgacctt gaggaaatgc
    17281 tggcggaacg cggcatttcg gtcgaccata cgacgatcta tcgctgggtc cagtgctacg
    17341 ccccggagat ggagaagcgg ctgcgctggt tctggcggcg tggctttgat ccgagctggc
    17401 gcctggatga aacctacgtc aaggtgcggg gcaagtggac ctacctgtac cgggcagtcg
    17461 acaagcgggg cgacacgatc gatttctacc tgtcgccgac ccgcagcgcc aaggcagcga
    17521 agcggttcct gggcaaggcc ctgcgaggcc tgaagcactg ggaaaagcct gccacgctca
    17581 ataccgacaa agcgccgagc tatggtgcag cgatcaccga attgaagcgc gaaggaaagc
    17641 tggaccggga gacggcccac cggcaggtga agtatctcaa taacgtgatc gaggccgatc
    17701 acggaaagct caagatactg atcaagccgg tgcgcggttt caaatcgatc cccacggcct
    17761 atgccacgat caagggattc gaagtcatgc gagccctgcg caaaggacag gctcgcccct
    17821 ggtgcctgca gcccggcatc aggggcgagg tgcgccttgt ggagagagct tttggcattg
    17881 ggccctcggc gctgacggag gccatgggca tgctcaacca ccatttcgca gcagccgcct
    17941 gatcggcgca gagcgacagc ctacctctga ctgccgccaa tctttgcaac agagcccgta
    18001 atagaacagg catcggcgct atgccaccga gcaagtccga cgggtgcgct tcatcaagcg
    18061 ggcgcaggcg ttgggtttca cgctgtccga gattggcgcg ttgctcgcac tggatgcggc
    18121 ttgcatctgt cacgacacgc gagcgctggc tgcccgtaag ttgggattga tcgagcagaa
    18181 gatggccgac ctcgccgcta tgcaggtggt acttggcggc ctgatgcgac agtgcgacgc
    18241 cggaagtggt ggggccgact gccccatcat tgacgcactg ataggcaatt cagactgttc
    18301 ataaaacggt ggttttctgg acaggtctgc agggtttcgc tttttcatgg ccgggtgtcg
    18361 ttttcagaag gcgactgcac gaaatatcag gagtcggctg cactgccaga actggccgcc
    18421 tgcaaggcag cgtagagagc ggtttttcct accttgaggc gtgcggcggc ctcgcggaca
    18481 ttcaagccgt tggcgacaag ctcccgcgct cgttgcaact tgtcggcagt gacaaccggc
    18541 ttgcgtccgc ctttgcgccc gcgtgcggcg gcagcggtca acccggcctt ggtgcgctcg
    18601 cggatcaggt cgcgctcgaa ctggcccagc gcaccgaaca catggaagat gagccgtccg
    18661 cccggcgtgg tggtgtcgat gttttcagtc aacgagcgga agccgacgcc ccgtgcttcc
    18721 agcttgccga ccgtctcaat caggtgcggc agggagcggc caagccgatc cagccgccag
    18781 acgaccagca catcgccatc gcgcagataa gccagcgcat cggccaagcc gggccggtct
    18841 gccttggccc cggatgccgt gtcctcgaaa acccgttcgc agcctgcatt gcgcagcgca
    18901 tccgtctgca aggcggtgtc ctgttccgcc gtcgataccc gcgcatagcc gatgagtgcc
    18961 atttccgccc atttgtccgt tataccgtcc gcctatctta ttgtccgtca tcccgttgcg
    19021 caagatgttt tcggacaggc gtccggtttg gccgcctaat gatcgtttgg cggacaaggc
    19081 gactggcatc agccgaacag ctcgctatgg gagccaagcc gcgccagtcg cagggtgtcg
    19141 gcgtcggact tgcggtagat cagcagcagg tcgggcttga tgtggcattc gcggtagccc
    19201 gcccaatcgc cggaaagatc gtggtcgcgg tagcgcacat ccaggggctg atcggtcgcc
    19261 agcgcgacca gaaccggctt gaggtcatca ttcatgcagt ggactcctga cactgttcgt
    19321 tcaatagacg actgcacaac agccttcaaa ccgcgttgtc tggtgcagtc gtcttctgaa
    19381 aatgacaccg ggcataccca aaaccgttaa gataaccgtc aatttttcta tgttcaggca
    19441 ctgtcaatga atcaacgcat tggatacgcc cgcgtttcga cggacgacca gcacctagac
    19501 ctgcagcgcg acgcgctcca gcaggccggg tgcagcgtga tctacgagga agcggccagc
    19561 ggcaagagcg cggcacgccc ggagcttgag cagtgccgca aggcgttacg ggccggggat
    19621 accctcgtgg tgtggcggct tgatcgactc gggcgcagcc tgcatgacct ggtgcagatc
    19681 gtggccgagc ttgagcagcg cggcgtgcat ttcgagagcc tgaccgagaa gatcgagacg
    19741 ggcagcgcat cgggcaagct gcaattccat gtattcgccg cactggccga gttcgagcgc
    19801 ggcttgatcc gtgagcggac gcaggccggg ctggcagcag cccgcgcccg tggccgggcc
    19861 ggtggccgca agccgaagtt agacgaccag caggtgcgag agatcaaagc cctgttgcgc
    19921 gatccagata ttaaggtggc cgaggtagcc cgccgttatg gcgtgtcccg aaccaccctt
    19981 tacaagcacg tcggcgtcat cacgccgcgt caatgaggac ttgggatgct ccaacaacta
    20041 gacaccgcag acagcgtgcg ccgcgaggtg gcaccccgta ctgcgcagaa acacaaagcc
    20101 gagttcggcc agttcatgac gccttcgagc gtggcccgct tcatggcgtc cctgttccca
    20161 ccgagcaccc tgcagacctg ccgcctactg gacgcaggcg cgggcgtggg cgcgttgtcc
    20221 tgcgcctttc tcgaccgctg ggtgacgggc ggctttggct ttgagtctgt cgaggcgacg
    20281 gcctacgaga tcgacgagaa gctgcgcggc caccttgcac agcacttggc cggatacagc
    20341 cgcgtgacgc cccgcatcat cgcgggcgat tacatcgagc tggcgaccgc cgaaggtctg
    20401 caggatcgag gctataccca tgcgatcctc aatccgcctt acaagaagat caacagccaa
    20461 tcagaccacc ggctggccct gcgcactgtc ggcatcgaga cggtgaatct gtattccgcc
    20521 tttgtcgcgc tggccgtggg cgaggcagcg ccgggcgggc agattgtggc catcatcccg
    20581 cgcagcttct gcaacgggcc gtattaccgc ccgttccgtg acttcatcct tgagcgggcg
    20641 gcgatccgcc acatgcacct gttcgagtcg cgcaacaagg ctttcaggga tgacgaggtg
    20701 ctgcaggaaa acatcatcat ccgcttggag cgtggcggcc agcaggggcc ggtcacggtc
    20761 acgacttcga ccgatgacag cttttccgac ctgaccaccc acgaacaccc gttcgaccgg
    20821 atcgtgttcc cggatgaccc ggagcggttc attcatgtgc cgaccaccac cgagaaaagc
    20881 gccatcgagc tatcgcccgc cgtgcgctac tcgctggccg acattggcgt gaaggtatcg
    20941 accgggccgg tagtcgattt tcggctgaaa gcgcacctgc gcgacatgcc agagccgggc
    21001 accgtgcccc tgatctaccc cggacacctg agcatcaccg ggacggtgtg gccggtgccg
    21061 ggcttgaaga agcccaacgc gatcatgcgc aacgacgaga cggaaaagtg gctttacccg
    21121 aacgggtttt attgcgtggt gcgccgcttc tcgtcgaagg aggaaaagcg ccgcgtggtg
    21181 gcaagcgtgg ttgatcctgc cgcctttggc gatcattccg tgctgggctt cgagaaccac
    21241 atgaacctct ttcacgagga caaacacggc ttgcccgagg cgctggcccg tgggctggcc
    21301 gtgttcctga acacgaccgc agtagatgag catttccggc gcttcaacgg ccatacgcag
    21361 gtcaatgcaa ccgacctcaa gctgatgaag tacccgagcc gtgacaccct gaccgagctt
    21421 ggcaagtggg ccatgcagca aggaacgctc acgcaagaac agatcgacgc caagctagga
    21481 accctgaccg catgaacaac caaaacgact acatcgaggc cgcgcagcaa atcatcgttt
    21541 ccttgggcct gccccgagcg cagcagaacg agcgttccgc cctgtgcctg ctggccctcc
    21601 tgaacctcac gccgggcaag gcatgggccg acgcggaaaa cccgcttgtg ggcatcacgc
    21661 ccatcatgga ttgggcacgg gagcactacg gcaaggagta cgcgcccaac acccgcgaga
    21721 cattccgccg ccagtccatg caccagttct gcgacgctgg cgtggccctc tacaacccgg
    21781 acaagcccga ccgcccggtg aatagcccga aggcggttta tcagatcgaa cccgccgcgc
    21841 tggccctgct gcgcaccttc ggcactccgg catggcatga cagcctcgcc gcctatctgg
    21901 ccgagcgtga aacgctggtt gcccgctacg ccaaggagcg cgagcagaac cgcatcccgg
    21961 ttgagattgc accgggccag caaatcaccc tcagccccgg cgagcacagc gaactgatcc
    22021 gggccatcat cgaggacttc gccccgcgct ttgcgccggg tagcgtgctg gtctatgccg
    22081 gcgacacggg cgacaagtgg ggctacttcg acgccgccct gctggccggg ctgggtgtcg
    22141 atgtggattc acacggcaag atgcctgacg tggtgctgca cttcaccgcg aaaaactggc
    22201 tgctactggt ggagtccgtc accagtcacg ggccggtcga tggaaagcgg cacgccgagc
    22261 ttgccaagct gtttgccgga tcgactgccg ggctggtcta tgtgaccgcc ttcccgaacc
    22321 gggccatcat gggccgctac cttggggaaa tcgcatggga aaccgaggta tgggtggccg
    22381 atgcgccctc tcacctgatc cacttcaacg gggtgcggtt ccttggcccc taccctaccg
    22441 aatgagcaca ggaggcccga ccatgaccac acccaccacc gagacagcaa aagaggtgct
    22501 gcgcggctac ggacgcgcca acgaggccgc gctggcgtcc ctgatggacg tgcaagactg
    22561 gcagcgcatc gcccggcagg aacttgagcg ccgggctacc agcatcgtgc aggcgctgga
    22621 tgatgcgacc cttgaggcca tcgcagcggg cacgctcgac atgcgggcca tgtgccgaca
    22681 ggtggccgac gagatccacc agaccgccta agccctcccc tgccccgcca ccgaaccccg
    22741 ccgcgtgcgg ggtttttcat ggcttcaaat cctcaaaagg tgcatttact caaatcctca
    22801 atatgctata ctgcgcttga gtaattcctt ttttcctcaa acgctcaata gctcaaggag
    22861 tgcatatgca agtcattgcc gtactgaacc agaagggcgg ggcgggtaaa accaccatcg
    22921 ccacccacct tgcccgcgcc ctgcaactcg acggcgcaga cgtgttgctg gttgattccg
    22981 acccgcaggg cagcgcccgc gattgggccg ccgtccgcga ggatcaaccc gtgcccgtgg
    23041 ttggcatcga ccggcccacc atcgagcgcg atctaaagag cgtggcccgg aaagactttg
    23101 ttgtgatcga cggagcgccg caggctcacg atctcgccgt gtcggcaatg aaggccgccg
    23161 attgcgtgct gatcccggtg cagccgtcgc cctacgacat ttgggcaacc tctgacctcg
    23221 tggatttggt caagcagcgc atcgagctta ccgacggcaa gctgaaagcg gctttcgtgg
    23281 tcagccgggc gatcaagggc accaagattg gcgcggaagt ctccgaggcg ctggccgggt
    23341 acggcctgcc gatcctgcaa acgcgcatca cgcagcgcgt catctacccg agcagcgcgg
    23401 caagcggcac cacggccatt gaccaagagc cgacgagcga ggccgccgag gaaatccggg
    23461 cgctggctga ggaagtgcgc cgtttcctca attgcaccat ttgagtaatt aaggagtagg
    23521 tgaaaatgag tagcgggaaa cttggcatga aagcaggccg cccgagcgcc gcgaaggctg
    23581 gccctaccct gtccgacctt gccgacaagg ccgccaccgt ccgggtgaat ttcgacctcg
    23641 accgggccga gcatacgaag ctgaagattt acgccgccca aaccgggcgc agcatcacgg
    23701 acattctgcg cgaactggtg cgctcacttg atgaaaagag tgattgagta attgagtgcg
    23761 gcgacgcggg gcaaggttta gcgcctgcaa gcatggactc tagtccatac tttattgaag
    23821 ggtgcgagat tgaataatgt tgatgaaaaa ccgggcgtga ggtataaagc gatggttgag
    23881 tctatcgagc cattgaagtt gcaagtgcta ccccctgccg tgttcaagga gtttgccgcg
    23941 gcgactcccc acgaaatcca cggggcagca gtgcgaaaca cggagcaggg cattgtcatc
    24001 gttctgaaca tcgggaacga gcggcgcatc ctcgggcagt accggggggg aatccgcttc
    24061 tttcgttcgt tcgatggtgc agcggcagtg ctcaggcagc atggggtgct gcaatggacg
    24121 gcggatgcaa caggatggat tccgcgcacc ttggaggcca aggagcggag ttcaacagga
    24181 tgaaaaagca aagccccgcg tggcagcggg gcttggcaag agggcagggc gctagacagg
    24241 tggcactgtt tagaacccca aggcgtgacc gatgcaggca gaccagacac accgaactat
    24301 ggtctgcatt gtagcgccgt accccttgcc gcacaagcgg aaagaggctc
//
