LOCUS       NZ_CP078001             2953 bp    DNA     circular CON 13-OCT-2023
DEFINITION  Pseudomonas aeruginosa strain SRRSH1120 plasmid p2, complete
            sequence.
ACCESSION   NZ_CP078001
VERSION     NZ_CP078001.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19950568
            Assembly: GCF_019857545.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Zhu,Y., Chen,J., Shen,H., Chen,Z., Yang,Q.-w., Zhu,J., Li,X.,
            Yang,Q., Zhao,F., Ji,J., Cai,H., Li,Y., Zhang,L., Leptihn,S., Hua,X.
            and Yu,Y.
  TITLE     Deciphering plasmidome of KPC-producing Pseudomonas aeruginosa
            reveals the blaKPC-2 copy number correlates with
            ceftazidime-avibactam resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Zhu,Y., Chen,J., Shen,H., Chen,Z., Yang,Q.-w., Zhu,J., Li,X.,
            Yang,Q., Zhao,F., Ji,J., Cai,H., Li,Y., Zhang,L., Leptihn,S., Hua,X.
            and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, Zhejiang University, 3 East Qingchun Road, Hangzhou,
            Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 218x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/13/2023 02:54:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,662
            CDSs (total)                      :: 6,582
            Genes (coding)                    :: 6,516
            CDSs (with protein)               :: 6,516
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 47 of 66
            Pseudo Genes (internal stop)      :: 4 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP078001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="SRRSH1120"
                     /host="Homo sapiens"
                     /db_xref="taxon:287"
                     /plasmid="p2"
                     /geo_loc_name="China:Hangzhou"
                     /lat_lon="30.28 N 120.15 E"
                     /collection_date="2018-09-10"
     gene            join(2893..2953,1..491)
                     /locus_tag="KUU71_RS33195"
                     /old_locus_tag="KUU71_33190"
     CDS             join(2893..2953,1..491)
                     /locus_tag="KUU71_RS33195"
                     /old_locus_tag="KUU71_33190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA plasmid replication protein"
                     /protein_id="WP_033995415.1"
                     /translation="MQQITVATDAQRRIRQRELAMLDEKEAQEREEARKNKGFTQVYPL
                     GWKRIIELAKGNAGAFGLYSFFAENIDPTCGAVVCDQQFLADKMNVNRRTISRWLSYLE
                     ENRALVRIPVAGKVCAYALDPHEVWKGYDNAKDYAAFVTKTLVNKDGDIRRRIMSMFSG
                     EKQNDDRDPNTIDMFESTPQ"
                     /name="RepA plasmid replication protein"
     gene            488..838
                     /locus_tag="KUU71_RS33200"
                     /old_locus_tag="KUU71_33195"
     CDS             488..838
                     /locus_tag="KUU71_RS33200"
                     /old_locus_tag="KUU71_33195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="WP_077265610.1"
                     /translation="MSNETKEKIFMKKTFALAAMMAAIAPNLASAADEMIQTKYACEGG
                     KTLDVVYVGDYAVMLQMDELVPLKRAKSASGVRYISASKNYTYELWGKGNDMNLSTHDG
                     GKEQTVLSSCKP"
                     /name="MliC family protein"
     gene            complement(865..1839)
                     /gene="mobV"
                     /locus_tag="KUU71_RS33205"
                     /old_locus_tag="KUU71_33200"
     CDS             complement(865..1839)
                     /gene="mobV"
                     /locus_tag="KUU71_RS33205"
                     /old_locus_tag="KUU71_33200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_033995413.1"
                     /translation="MAAYAIMRCKKLATMGSVAAALQHCYRERETPNADAERTPDNEHR
                     AATSTDAAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWAKATPQQQAAFFDQAQGWLA
                     AKYGADRIVTASIHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRDKMRADQTSFAEAVR
                     DLGLERGIEGSRATHQRVQSYYGAIERQPGHATITPQAIEPRVLRKGIFSKDVETPEAV
                     AARVTAAVREGYGPTVAAAAGARQEREKARQAQETARSLRDRLKPVLDALGPLNRDMQA
                     KAAQIIKAVGEKLLAEQREATRQRQAQRLRSRGRDRDDGGLSR"
                     /name="mobV"
ORIGIN
        1 tgctggacga gaaagaggct caggagcgcg aagaggcgcg gaagaacaag gggttcacgc
       61 aggtgtatcc cttgggttgg aagcgcatca ttgagcttgc taaaggcaat gctggggcct
      121 tcggcctgta ttcattcttt gctgaaaata tcgacccaac ctgcggcgcg gtggtctgcg
      181 atcagcagtt tttggcagac aagatgaacg tcaacaggcg gacaatcagc cgttggttgt
      241 cttacctgga agaaaatcgc gcgcttgtcc gcatccctgt tgctggcaag gtttgcgcct
      301 atgcgctgga tcctcacgag gtctggaagg gctacgacaa cgccaaggat tacgcagctt
      361 tcgtgaccaa aaccttggtc aacaaggatg gcgatatccg ccgccggatc atgtcgatgt
      421 tcagcggcga gaagcagaac gacgaccgcg acccgaacac cattgacatg tttgaaagca
      481 ctccccaatg agcaacgaaa cgaaggagaa aatctttatg aaaaaaacgt tcgctcttgc
      541 tgccatgatg gcggcaatcg ctccgaatct tgcttcagca gcggatgaga tgatccagac
      601 caagtacgct tgcgaaggcg gaaagacgtt ggatgtcgtg tacgtcggag actatgccgt
      661 gatgctgcaa atggatgagc ttgtcccact gaaacgcgcc aagagcgcat caggagtgcg
      721 ctacatctcc gctagcaaaa actacaccta cgaactgtgg ggtaagggca acgacatgaa
      781 cctttcgacc cacgatggcg ggaaggagca aaccgtcctc agtagctgca agccataaac
      841 cccttccgtc ggccacctgt agggtcaacg agacagcccg ccgtcgtcgc gatcccggcc
      901 gcgactgcga agccgttggg cctgccgctg gcgtgtcgcc tcgcgctgtt cggcgagcag
      961 cttctcgccc acggccttga tgatctgggc cgccttggcc tgcatatcgc gattaagcgg
     1021 ccccagagcg tccagaacgg gcttcaggcg gtcacgcagg ctccgggctg tctcctgtgc
     1081 ctgccgggcc ttctcgcgct cctgacgcgc tcctgcggcc gctgcgacgg tcgggccata
     1141 cccttccctg acggcagcgg tcacgcgggc ggccacggcc tccggcgtct ccacgtcctt
     1201 cgagaaaatc cccttgcgta gcacccgcgg ctcgatggcc tgcggcgtga tggtggcgtg
     1261 cccgggctgg cgctcgatgg cgccgtagta gctctggacg cgctgatggg tggcccggct
     1321 gccctcgatg ccgcgttcca gcccgagatc cctcaccgcc tcggcaaagc tggtctggtc
     1381 ggcccgcatc ttgtcgcggc caccgatgaa ctccttggcc gacagccgcc cgtcctgcgt
     1441 cagcggcacg acgaaggccg acaggtgcgg ggtggcctcg tcgcggtgga tgctggccgt
     1501 cacgatgcga tccgcgccgt atttggccgc cagccagccc tgcgcctggt cgaagaacgc
     1561 ggcctgctgc tggggcgtgg ccttggccca ccattccggg ctggccgtca tcacgtattc
     1621 gaccgccagc accgcgtcct tgcgccgctt ctccggcagc aactcgcgca gccgccccat
     1681 cgccgcatcg gttgaggtgg ccgcccggtg ctcgttgtcc ggcgtgcgtt cggcgtcagc
     1741 gttgggcgtt tcgcgctcgc ggtagcagtg ctgcaacgcc gccgccacgc tgcccatcgt
     1801 cgccagcttc ttgcatcgca tgatcgcgta ggccgccatg cctgcccctc cccttggtgt
     1861 ccgaatccgg ccagtcgggg cagcgcaagg cggtgcctcc ggcgggccac tatcagatga
     1921 tagtgtactc actagacttt gcgaagcaaa gtcgtgcccg cctgcggcgg ttgcagcgcc
     1981 cttcgggctt gctcctgcgg cggccttcgg ccttgcccaa aggggctttg cccctctgga
     2041 ctccccaccc tcgccgccgg gaggctcggg gggcaggggt ggcgggcttc gcccttcgtc
     2101 cctgccccca ctcgcacagg ctggggcgct gcggtcagcg tcaagggttc ggcttcgccc
     2161 gcgtcctcgc ccggtccctg cgcttcgctc cgggctgcgg cctgcgggcg cgcccttgac
     2221 cctgccctcc gctgaaggcg tcagccagtg gcccgctggc gtgtcggaaa tcgcctcatg
     2281 gccgggtagg aaatttccga cagcgccgga gtggaaggcg ggtcaagggc gcagggggtg
     2341 gctggcgcgg cccgcagccc gcagggcgag gacacgggcc acccccggag agcgcagcgg
     2401 cccttgacgc gcctggaacg accccacgct ccccggtagg ggtgcaggag tgaagggccg
     2461 caggcccgta gcccctgcac ccctgccacg tggcgaacgc ggggggtggc ggggggcagc
     2521 gcccaccgcc ccaaggctgc gcagcggccg ccggaggccc cccaaacaac gtcacccggc
     2581 cggtggccgg gtgggccttt tccagaggcc ccgcaggggc cgtcagcggg gtgccaaggc
     2641 cgcgtagcgg ccgccggcgg ggcggcagcc ccttagagga gaaaggggga aatgctaagt
     2701 tattgattta taaggatttg tagaaatgca ttgagacaca tagggtgtcc taacgttgag
     2761 acatgcatgg atgtctcaac aggtagcaag gttgagacgc aaactgtctc gacgtgagac
     2821 ataatttgtc gtatcattgc gactcgaaat ggcgcaatga gcggacaaat ttcccgactt
     2881 tggaggaacc ccatgcagca gatcacggtg gcgaccgacg cccagcgtcg tatccgccag
     2941 cgagagctgg cca
//
