LOCUS       NZ_CP077993             2953 bp    DNA     circular CON 13-OCT-2023
DEFINITION  Pseudomonas aeruginosa strain ZPPH1 plasmid p5, complete sequence.
ACCESSION   NZ_CP077993
VERSION     NZ_CP077993.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19950571
            Assembly: GCF_019857485.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Zhu,Y., Chen,J., Shen,H., Chen,Z., Yang,Q.-w., Zhu,J., Li,X.,
            Yang,Q., Zhao,F., Ji,J., Cai,H., Li,Y., Zhang,L., Leptihn,S., Hua,X.
            and Yu,Y.
  TITLE     Deciphering plasmidome of KPC-producing Pseudomonas aeruginosa
            reveals the blaKPC-2 copy number correlates with
            ceftazidime-avibactam resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Zhu,Y., Chen,J., Shen,H., Chen,Z., Yang,Q.-w., Zhu,J., Li,X.,
            Yang,Q., Zhao,F., Ji,J., Cai,H., Li,Y., Zhang,L., Leptihn,S., Hua,X.
            and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, Zhejiang University, 3 East Qingchun Road, Hangzhou,
            Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/13/2023 02:50:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,456
            CDSs (total)                      :: 6,376
            Genes (coding)                    :: 6,314
            CDSs (with protein)               :: 6,314
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 25 of 62
            Pseudo Genes (incomplete)         :: 44 of 62
            Pseudo Genes (internal stop)      :: 4 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077993.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="ZPPH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:287"
                     /plasmid="p5"
                     /geo_loc_name="China:Hangzhou"
                     /lat_lon="30.28 N 120.18 E"
                     /collection_date="2017-07-05"
     gene            join(2837..2953,1..858)
                     /gene="mobV"
                     /locus_tag="KUU79_RS32165"
                     /old_locus_tag="KUU79_32150"
     CDS             join(2837..2953,1..858)
                     /gene="mobV"
                     /locus_tag="KUU79_RS32165"
                     /old_locus_tag="KUU79_32150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_033995413.1"
                     /translation="MAAYAIMRCKKLATMGSVAAALQHCYRERETPNADAERTPDNEHR
                     AATSTDAAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWAKATPQQQAAFFDQAQGWLA
                     AKYGADRIVTASIHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRDKMRADQTSFAEAVR
                     DLGLERGIEGSRATHQRVQSYYGAIERQPGHATITPQAIEPRVLRKGIFSKDVETPEAV
                     AARVTAAVREGYGPTVAAAAGARQEREKARQAQETARSLRDRLKPVLDALGPLNRDMQA
                     KAAQIIKAVGEKLLAEQREATRQRQAQRLRSRGRDRDDGGLSR"
                     /name="mobV"
     gene            complement(885..1235)
                     /locus_tag="KUU79_RS32170"
                     /old_locus_tag="KUU79_32155"
     CDS             complement(885..1235)
                     /locus_tag="KUU79_RS32170"
                     /old_locus_tag="KUU79_32155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="WP_077265610.1"
                     /translation="MSNETKEKIFMKKTFALAAMMAAIAPNLASAADEMIQTKYACEGG
                     KTLDVVYVGDYAVMLQMDELVPLKRAKSASGVRYISASKNYTYELWGKGNDMNLSTHDG
                     GKEQTVLSSCKP"
                     /name="MliC family protein"
     gene            complement(1232..1783)
                     /locus_tag="KUU79_RS32175"
                     /old_locus_tag="KUU79_32160"
     CDS             complement(1232..1783)
                     /locus_tag="KUU79_RS32175"
                     /old_locus_tag="KUU79_32160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA plasmid replication protein"
                     /protein_id="WP_033995415.1"
                     /translation="MQQITVATDAQRRIRQRELAMLDEKEAQEREEARKNKGFTQVYPL
                     GWKRIIELAKGNAGAFGLYSFFAENIDPTCGAVVCDQQFLADKMNVNRRTISRWLSYLE
                     ENRALVRIPVAGKVCAYALDPHEVWKGYDNAKDYAAFVTKTLVNKDGDIRRRIMSMFSG
                     EKQNDDRDPNTIDMFESTPQ"
                     /name="RepA plasmid replication protein"
ORIGIN
        1 ccggacaacg agcaccgggc ggccacctca accgatgcgg cgatggggcg gctgcgcgag
       61 ttgctgccgg agaagcggcg caaggacgcg gtgctggcgg tcgaatacgt gatgacggcc
      121 agcccggaat ggtgggccaa ggccacgccc cagcagcagg ccgcgttctt cgaccaggcg
      181 cagggctggc tggcggccaa atacggcgcg gatcgcatcg tgacggccag catccaccgc
      241 gacgaggcca ccccgcacct gtcggccttc gtcgtgccgc tgacgcagga cgggcggctg
      301 tcggccaagg agttcatcgg tggccgcgac aagatgcggg ccgaccagac cagctttgcc
      361 gaggcggtga gggatctcgg gctggaacgc ggcatcgagg gcagccgggc cacccatcag
      421 cgcgtccaga gctactacgg cgccatcgag cgccagcccg ggcacgccac catcacgccg
      481 caggccatcg agccgcgggt gctgcgcaag gggattttct cgaaggacgt ggagacgccg
      541 gaggccgtgg ccgcccgcgt gaccgctgcc gtcagggaag ggtatggccc gaccgtcgca
      601 gcggccgcag gagcgcgtca ggagcgcgag aaggcccggc aggcacagga gacagcccgg
      661 agcctgcgtg accgcctgaa gcccgttctg gacgctctgg ggccgcttaa tcgcgatatg
      721 caggccaagg cggcccagat catcaaggcc gtgggcgaga agctgctcgc cgaacagcgc
      781 gaggcgacac gccagcggca ggcccaacgg cttcgcagtc gcggccggga tcgcgacgac
      841 ggcgggctgt ctcgttgacc ctacaggtgg ccgacggaag gggtttatgg cttgcagcta
      901 ctgaggacgg tttgctcctt cccgccatcg tgggtcgaaa ggttcatgtc gttgccctta
      961 ccccacagtt cgtaggtgta gtttttgcta gcggagatgt agcgcactcc tgatgcgctc
     1021 ttggcgcgtt tcagtgggac aagctcatcc atttgcagca tcacggcata gtctccgacg
     1081 tacacgacat ccaacgtctt tccgccttcg caagcgtact tggtctggat catctcatcc
     1141 gctgctgaag caagattcgg agcgattgcc gccatcatgg cagcaagagc gaacgttttt
     1201 ttcataaaga ttttctcctt cgtttcgttg ctcattgggg agtgctttca aacatgtcaa
     1261 tggtgttcgg gtcgcggtcg tcgttctgct tctcgccgct gaacatcgac atgatccggc
     1321 ggcggatatc gccatccttg ttgaccaagg ttttggtcac gaaagctgcg taatccttgg
     1381 cgttgtcgta gcccttccag acctcgtgag gatccagcgc ataggcgcaa accttgccag
     1441 caacagggat gcggacaagc gcgcgatttt cttccaggta agacaaccaa cggctgattg
     1501 tccgcctgtt gacgttcatc ttgtctgcca aaaactgctg atcgcagacc accgcgccgc
     1561 aggttgggtc gatattttca gcaaagaatg aatacaggcc gaaggcccca gcattgcctt
     1621 tagcaagctc aatgatgcgc ttccaaccca agggatacac ctgcgtgaac cccttgttct
     1681 tccgcgcctc ttcgcgctcc tgagcctctt tctcgtccag catggccagc tctcgctggc
     1741 ggatacgacg ctgggcgtcg gtcgccaccg tgatctgctg catggggttc ctccaaagtc
     1801 gggaaatttg tccgctcatt gcgccatttc gagtcgcaat gatacgacaa attatgtctc
     1861 acgtcgagac agtttgcgtc tcaaccttgc tacctgttga gacatccatg catgtctcaa
     1921 cgttaggaca ccctatgtgt ctcaatgcat ttctacaaat ccttataaat caataactta
     1981 gcatttcccc ctttctcctc taaggggctg ccgccccgcc ggcggccgcg tagcggcctt
     2041 ggcaccccgc tgacggcccc tgcggggcct ctggaaaagg cccacccggc caccggccgg
     2101 gtgacgttgt ttggggggcc tccggcggcc gctgcgcagc cttggggcgg tgggcgctgc
     2161 cccccgccac cccccgcgtt cgccacgtgg caggggtgca ggggctacgg gcctgcggcc
     2221 cttcactcct gcacccctac cggggagcgt ggggtcgttc caggcgcgtc aagggccgct
     2281 gcgctctccg ggggtggccc gtgtcctcgc cctgcgggct gcgggccgcg ccagccaccc
     2341 cctgcgccct tgacccgcct tccactccgg cgctgtcgga aatttcctac ccggccatga
     2401 ggcgatttcc gacacgccag cgggccactg gctgacgcct tcagcggagg gcagggtcaa
     2461 gggcgcgccc gcaggccgca gcccggagcg aagcgcaggg accgggcgag gacgcgggcg
     2521 aagccgaacc cttgacgctg accgcagcgc cccagcctgt gcgagtgggg gcagggacga
     2581 agggcgaagc ccgccacccc tgccccccga gcctcccggc ggcgagggtg gggagtccag
     2641 aggggcaaag cccctttggg caaggccgaa ggccgccgca ggagcaagcc cgaagggcgc
     2701 tgcaaccgcc gcaggcgggc acgactttgc ttcgcaaagt ctagtgagta cactatcatc
     2761 tgatagtggc ccgccggagg caccgccttg cgctgccccg actggccgga ttcggacacc
     2821 aaggggaggg gcaggcatgg cggcctacgc gatcatgcga tgcaagaagc tggcgacgat
     2881 gggcagcgtg gcggcggcgt tgcagcactg ctaccgcgag cgcgaaacgc ccaacgctga
     2941 cgccgaacgc acg
//
