LOCUS       NZ_CP077984            12333 bp    DNA     circular CON 13-OCT-2023
DEFINITION  Pseudomonas aeruginosa strain ZPPH14 plasmid p2, complete sequence.
ACCESSION   NZ_CP077984
VERSION     NZ_CP077984.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19950573
            Assembly: GCF_019857445.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12333)
  AUTHORS   Zhu,Y., Chen,J., Shen,H., Chen,Z., Yang,Q.-w., Zhu,J., Li,X.,
            Yang,Q., Zhao,F., Ji,J., Cai,H., Li,Y., Zhang,L., Leptihn,S., Hua,X.
            and Yu,Y.
  TITLE     Deciphering plasmidome of KPC-producing Pseudomonas aeruginosa
            reveals the blaKPC-2 copy number correlates with
            ceftazidime-avibactam resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12333)
  AUTHORS   Zhu,Y., Chen,J., Shen,H., Chen,Z., Yang,Q.-w., Zhu,J., Li,X.,
            Yang,Q., Zhao,F., Ji,J., Cai,H., Li,Y., Zhang,L., Leptihn,S., Hua,X.
            and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, Zhejiang University, 3 East Qingchun Road, Hangzhou,
            Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 126x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/13/2023 02:58:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,973
            CDSs (total)                      :: 5,894
            Genes (coding)                    :: 5,833
            CDSs (with protein)               :: 5,833
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 31 of 61
            Pseudo Genes (incomplete)         :: 29 of 61
            Pseudo Genes (internal stop)      :: 9 of 61
            Pseudo Genes (multiple problems)  :: 7 of 61
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077984.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12333
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="ZPPH14"
                     /host="Homo sapiens"
                     /db_xref="taxon:287"
                     /plasmid="p2"
                     /geo_loc_name="China:Hangzhou"
                     /lat_lon="30.28 N 120.20 E"
                     /collection_date="2016-08-12"
     gene            join(12259..12333,1..969)
                     /locus_tag="KUU73_RS29715"
                     /old_locus_tag="KUU73_29705"
     CDS             join(12259..12333,1..969)
                     /locus_tag="KUU73_RS29715"
                     /old_locus_tag="KUU73_29705"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_222836370.1"
                     /translation="MHQLELFSPQTLPRRPYCTDDPASGMTPQPLQLALTYRHLQMNPP
                     NALFWLVVDVDAPVISDPISPQMRAIFDGTVPLPNFLAVNPANAHAHVYYALSRPVAKG
                     DHASIAATRYAAAIEAALIRALGGDPLYAGVIAKNPTHQHWRRVDLRSEPYTLHELEDY
                     LDLSGPSKSAQRAEALEECATRRNCWLFDQLRFHAYPLVSLYRQDANFNTWRRYLQACA
                     QGYNSELPAPLQANELSHIVKSVASWTWTQYSGLLSDEGFSKLQAHRGAKGGRISSLKR
                     AEAGTLEASMSAARAAKAARAADGDKAAEAARLQAEGVAVAEIARRLGVSRPTVYAYLK
                     KCKALKP"
                     /name="replication initiation protein"
     gene            complement(1186..4151)
                     /locus_tag="KUU73_RS29720"
                     /old_locus_tag="KUU73_29710"
                     /pseudo=""
     CDS             complement(1186..4151)
                     /locus_tag="KUU73_RS29720"
                     /old_locus_tag="KUU73_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            complement(4155..4715)
                     /locus_tag="KUU73_RS29725"
                     /old_locus_tag="KUU73_29715"
     CDS             complement(4155..4715)
                     /locus_tag="KUU73_RS29725"
                     /old_locus_tag="KUU73_29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_172689450.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQVEVGKLFTDKASGKDTQRPQL
                     EAMLGFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAITLRQRAAAGEP
                     KAQLAREFGISRETLYQYLRKDD"
                     /name="recombinase family protein"
     gene            complement(5117..5347)
                     /locus_tag="KUU73_RS29730"
                     /old_locus_tag="KUU73_29720"
     CDS             complement(5117..5347)
                     /locus_tag="KUU73_RS29730"
                     /old_locus_tag="KUU73_29720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_222836371.1"
                     /translation="MKTTLDHDALQALQEAGALRELRAIRRAPGWQLQARLATRWLDVR
                     SRREPVRIWGSLDSLAKFCDALGVRCLSVEL"
                     /name="hypothetical protein"
     gene            5869..6459
                     /locus_tag="KUU73_RS29735"
                     /old_locus_tag="KUU73_29725"
     CDS             5869..6459
                     /locus_tag="KUU73_RS29735"
                     /old_locus_tag="KUU73_29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_222836372.1"
                     /translation="MANGKQASAKKLKMAEKAQQLMGVHFPGIPPQWVWHRKTNDGYST
                     VPRTLPIAMQAIDTQSKGQPAGHTLLCLWARAPDHPVITIENPTTFAAEAGFLGERAVD
                     TWRRRMRTLRDLHFIQTKPGASGEFHYVLLMNPNAAVERMRVSGQVSDGVYGRFIDRLI
                     EVGAFGEIEAIRELWEAQRAAAPAVAEAAPSTS"
                     /name="hypothetical protein"
     gene            complement(7385..7915)
                     /locus_tag="KUU73_RS29740"
                     /old_locus_tag="KUU73_29730"
     CDS             complement(7385..7915)
                     /locus_tag="KUU73_RS29740"
                     /old_locus_tag="KUU73_29730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="WP_222836364.1"
                     /translation="MNAPGHVKELPGQKTWVDYGLADLRSLPTELRSQAPVEIPAADSV
                     DAAVELVAGVFNLGDPTIDRTAMTTPVGLLTVVRDKLVHIVEKRPDARERYTNHAVSTL
                     NDPYEVWKVEYDNGNHRLAFIGAFEGKRQMLVIVDTIDGQVLWNFMHTDAKSLNKHRHG
                     LPIYQRSPPENKA"
                     /name="PBECR2 nuclease fold domain-containing protein"
     gene            complement(8268..8555)
                     /locus_tag="KUU73_RS29745"
                     /old_locus_tag="KUU73_29735"
     CDS             complement(8268..8555)
                     /locus_tag="KUU73_RS29745"
                     /old_locus_tag="KUU73_29735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_222836365.1"
                     /translation="MVKPRSATAAGSHQTATTEQAERLAQELADKPYGKRKDVPPIAPE
                     TNRKAVAVGISLPPDMIERLQDAAHQNKRAGSGQKTVSGIIRELLEQAGY"
                     /name="hypothetical protein"
     gene            complement(8548..9189)
                     /locus_tag="KUU73_RS29750"
                     /old_locus_tag="KUU73_29740"
     CDS             complement(8548..9189)
                     /locus_tag="KUU73_RS29750"
                     /old_locus_tag="KUU73_29740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_222836366.1"
                     /translation="MITIVGCNKGGASKTTTATNIAVALAKAGRDVVLVDADPQRTAAK
                     WHAEREASGISPAFPLIEKRDNITSAIRALAEKFEHVVVDVAGRNSRELITGCVVADVL
                     LAPHQCSQFDLDTLEELQEQVAKIRDLNPDLRVLVYHAMASTNPSVKGRERAEFAGYLA
                     EFPEFELLDAVGYYRKAYKDAASEGRSVLETSNGEAASEIDALVREVYRG"
                     /name="AAA family ATPase"
     gene            complement(9352..9831)
                     /locus_tag="KUU73_RS29755"
                     /old_locus_tag="KUU73_29745"
     CDS             complement(9352..9831)
                     /locus_tag="KUU73_RS29755"
                     /old_locus_tag="KUU73_29745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_222836367.1"
                     /translation="MKVTTDKLLRPSKEELDLLKNLDELYQFGDGSEFDNLVRSALMDD
                     LRRGLIHPRQGGGLDVEERADSLHLRGRYYILKDAELPSEEHRDVFRRLCHQSPAMPFD
                     VDLPGGQVANGQLVEERDFQRLLRCIVALVEKLLGLGDVPERDKTHNLSDDFELK"
                     /name="hypothetical protein"
     gene            10017..10367
                     /locus_tag="KUU73_RS29760"
                     /old_locus_tag="KUU73_29750"
     CDS             10017..10367
                     /locus_tag="KUU73_RS29760"
                     /old_locus_tag="KUU73_29750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_222836368.1"
                     /translation="MSKKRRGPQPLPQEDKRVIRVVMKLTAEEYELAKIKAAGINLAKY
                     ARAALLQAKPAPPPPVLPTANVEVWKGSAALQNNLNQLVKRLHVQGLEAGDVEELAALV
                     ASLRAALVGVKS"
                     /name="hypothetical protein"
     gene            10373..11992
                     /locus_tag="KUU73_RS29765"
                     /old_locus_tag="KUU73_29755"
     CDS             10373..11992
                     /locus_tag="KUU73_RS29765"
                     /old_locus_tag="KUU73_29755"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_261993712.1"
                     /translation="MQKIKRGSGFRGVAAYMLNDKNAQLLGGNMSGRTAEELGREFNVS
                     RQLRPDIKRPVWHNSLRLPSGERLAPEQWCQVADEYMRRMGFSEAHQRVYVMHDDPQGQ
                     HVHIAASRVALDGQVYLGRNENLLSTRHIQALEHDFGLTVTPGPTLENGRIRAPDIRRP
                     TAGEVGRYERTGESPARYQLAGLIDQALQDRPTAVQFVERLQLAGVEVRPNINKAGLSG
                     FSFSINGVAFKGSQLGDKYKAKALISRGLNYEQDRDYEWLKRLAASIGNCQSSRGNAAD
                     NAGPGDDTIRGTSAPGASDSCERVGEAVLHGNTTNTNRRDSDAGEVDRSHWRGESINRS
                     HSETSGGTEAERNAIQLAETSRRPGGQNKGPGNASNHQHMHSSRKRAGAPNTALEASLK
                     CQEAATTRAADRMLSLMATAIRKALRGGAPGELTYGLIQFSRQFLEQAKTPRREPVQER
                     QPQVDQQPAPEALQGPQEAPRQVLEGLALDHALQGLEQAPSRIEQHRQALAERERAEIK
                     AAQRKQREREAGVLEGPELSLR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
ORIGIN
        1 atgactcctc agccgctgca gttggcgttg acctatcgac acctgcagat gaacccgccg
       61 aacgcgttgt tctggctggt cgttgacgtt gacgcgcccg tgatcagtga tccgatttcg
      121 ccgcagatgc gcgcgatttt cgacggcacc gtgccactgc cgaacttcct cgcggtaaac
      181 cctgcgaatg ctcacgcaca tgtctactac gcgctctcta ggccggtagc caagggcgat
      241 catgcgagca ttgcggcaac gcgctacgcc gccgccatcg aagccgcgct gattcgggct
      301 ctcgggggcg atccgctgta tgccggcgtg atcgcgaaaa acccgaccca ccagcattgg
      361 cggcgcgtgg atcttcgctc ggagccctac accctgcacg agctcgagga ctacctcgac
      421 ctcagcggcc cgagcaaatc tgcgcaacgc gccgaggccc tggaagagtg cgcgacgcga
      481 cgcaactgct ggctgttcga tcagttgcgg ttccacgcct acccgttggt ctcgctgtac
      541 cgtcaggacg cgaatttcaa cacctggcgg cgctaccttc aggcctgcgc ccagggctac
      601 aacagcgagc taccggcgcc gctgcaggcc aacgagctga gccacatcgt gaaatcggtg
      661 gcctcgtgga cgtggacgca gtacagcggt ctgctcagcg acgaggggtt cagcaagctt
      721 caagcgcatc gaggcgccaa aggaggccgc atcagctccc tgaagcgggc cgaagctgga
      781 acgttggagg caagcatgag cgctgcccgt gccgcgaagg ctgctagggc ggcagatgga
      841 gacaaggcag cggaggctgc ccgcctgcag gcggagggcg tcgcggttgc agagattgcc
      901 cgccgcctgg gggtgagtcg gccaactgtc tacgcctacc tgaaaaagtg taaagctctg
      961 aagccatagt cagacaggcc cccgtcaggg ggccgctctt gctcttcctt aaaccacgga
     1021 gccccgtagg ggcgacagcc ttttcctgcg ctctaccctg taaaccccct agaccagacc
     1081 tctgcagctc taacggcaaa agggaaaaca ggctcctgac tgtcagtgct cccccatgca
     1141 catagctatc tgggggcgaa tagagaaaac ggaaaaaatc gtacgctaag gttttccggg
     1201 cagccttagc ggccgaaact tcccgtcctc cagcctgcgg ctctgccgcc agacataatc
     1261 gccggtcaga ttgatgtgct cccaccccag cggcgacagg tattgcagca gctcaccatc
     1321 caccgacttc cccgcatcgc ccatcgcctg ggtggcgcgc tccaagtaca ccgtgttcca
     1381 caacacgatg gcggccgtca ccaggttgag gccactggcc cggtagcgct gctgctcgaa
     1441 gctccgatcc ctgatctcgc cgaggcggtt gaagaacacc gccctggcca gggagttgcg
     1501 ggcttcgccc ttgttcagtc cagcatgcac gcgccggcgc agctcgacgc tttgcaacca
     1561 atcgaggatg aaaagcgtgc gctcgatccg gcccagctcg cgcagggcca cggccaggcc
     1621 gttctggcgc ggatagctgc cgagcttgcg cagcatcagc gaggcggtga ccgtgccctg
     1681 cttgatcgag ctggccaggc gcaggatgtc gtcccaatgg gcgcggacgt gcttgatgtt
     1741 cagggtgccg ccaaccattg ggcgcaatgt cgggtagtcc tggacgctat tcggaacata
     1801 cagcttggtt tctccgaggt cgcggatgcg cggtgcgaag cggaagccca gcaggtgcat
     1861 cagggcgaag acatgatcgg tgaagccagc cgtgtcagtg tagtgctcct cgatccgcaa
     1921 gtcggactcg tggtacagca gaccgtcgag cacataggta gagtcgcgca cgccgacatt
     1981 caccacgcgg gtgctgaacg gtgcgtactg gtcggagatg tgggtgtaga acagccgccc
     2041 cggctcgctg ccgtacttcg ggttgacgtg gccggtgctt tcgcctcgac cacccgcccg
     2101 gaagcgctgg ccatcggaag aagaggtcgt gccgtcgccc cagtgagcgg cgaaggtatg
     2161 acggtactgg tggttgacca gctcggccag ggccgccgag taggtttcgt ctcggatgtg
     2221 ccaggcttgc agccaggaca gcttggcgta ggtcaggccg gggctcgact cggccatctt
     2281 ggtcagcccg aggttgattg catcgcccag gatcgctgaa agcagcaatg tccggtcttt
     2341 ggcctcggca ccgtccttca ggtgtgtgaa gtggcggctg aagcccgtcc agtcgtccac
     2401 gtccatcagc agttcggtga tcttgatgcg cggcagcaaa tggctggtct ggtcgatcag
     2461 cgcctgcgcg gtattgggca ccgcggaatc cagcggggtg atcttcagcc cggactcggt
     2521 gaggatggca tcgggcagct cgttgtcctt ggccaggcgg gtgacggtgg ccagctgctc
     2581 gtccagcagc tgcaagcgct cttccaggta ctggttgctg ttcgggttga tcgccagggg
     2641 cagagcctgc gcatgcttga gcgcggcgaa cctctctgcc ggcagcaggt agtcgtcgaa
     2701 gtcgcggaac tgccgcgagc ccttgaccca gatgtcaccg gagcgcagcg cgttcttcag
     2761 ctcggacagg gcgcagattt cgtagaagcg ccggtcgagg ccttccgggg ttatcactag
     2821 cggcttccag cgcggcttga tgaaggcggt gggcgcatcg gccggcacct tgcgcaggtt
     2881 gtaggcgttc atctcgctca gggtctgcac ggctgccagc acgccttgcg cagccggggc
     2941 agcgcgcagt tccagcacct ccagcaaggc cggcgtgtaa cggcgcagag tggcgaagtt
     3001 ctcgccgacc aggtgcagat ggtcgaagcc ttccggccgg gccagcagct cggcctcgct
     3061 gacgctctcg gtgaactcgt cccagggaat caccgcctca atggcggcat agggatcgct
     3121 accgctttcc ttggcttcca gcagagcctg gccgatcttc gagtacaggc gcaccttgtc
     3181 gttgatcgcc ttgccctgct tctggaactg ctgctgatgc ttgtgcttcg cgctgctgaa
     3241 cagcttgacc aggatacggt cgtgcagatc gaccaactca tcaatcaccg tcgcggtgct
     3301 ctccagcacc acggcggcca gggtcgcgta gcgtcgctgc ggctcgaact tgccgaggtc
     3361 tttgggcgtc atctgcccac cctcgcgggc cagcttgagc aggcggttct ggtggatgtg
     3421 tcggcccagg ccttcgggca agtccaccag ctgaaatgtc ttcagccgct cgatgtgttc
     3481 gagcatgtgg cggagttcgg tttcagcggt gcctgtcgca gccaggtcaa ccaggtgatg
     3541 ctgctgccgg ccttgagctt caacagctcg tccagcttgg cccgatgcga gtccgtgagt
     3601 ggttcgacca gggcgcggta gactcggcga ttggcccgcg caatggcctc cgaacaagct
     3661 cgatcaatta cgctcaacgc cggcagaatg cgccgcttct gtcgcaggct ctccagggcc
     3721 tgaccggcca gcagtaagcc cttgtcggtc tgctgggcca actcggtcag ctcgcgcacc
     3781 agggcgcgga agtcggacag gccgaacggg gccagttgta ggtaggtgcg cagttcctgg
     3841 gcgtgctcgc ggcgagtcac gtcgcgttcg ccgtacttca accaactcgc cgggtcggcc
     3901 tgaacttgct tggccaccca ctggatgacc ggctcgggcg gctcgctgtc ggtgcccaac
     3961 gcatagccgg ggtagcgcag caggctgagc tgcaccgcga agcccaggcg gttggcgtcg
     4021 ccgcgccgct ggcggatcag cgacaggtcg gagtcgttga aggtgtagta gcggatcagg
     4081 tcatcctggc tttccggcaa cgcaagcaag gtgtcgcgct ccgtagccga gagaatcgag
     4141 cgacgcggca tggttcagtc gtccttgcga aggtactgat agagggtttc tcggctgatg
     4201 ccgaactcgc gggcgagctg cgccttcggc tcgcccgcag cggcccgctg ccgcagcgtg
     4261 atggcttgct cgtcggagag ggctttcttg cggccccggt aggcaccgcg ctgcttggcc
     4321 agggcgatgc cctcgcgctg ccgctcgcga atcagggcgc gctcaaactc ggcaaaggcc
     4381 cccatgaccg acagcatcag gttggccatc ggcgagtcct cgccggtgaa caccaggcct
     4441 tccttcaaga actcgatgcg cacaccgcgc tgggtcagct tctgcaccag gcggcgcaaa
     4501 tcatcgaggt ttcgtgccag gcgatccatg ctgtgcacca ccacggtgtc gccttcgcga
     4561 acgaaaccga gcatggcctc cagctgggga cgctgggtgt ccttgcccga ggctttgtcg
     4621 gtgaacagct tgccgacctc gacctgttca agttggcgtt ccgggttctg gtcaaagctg
     4681 ctgacccgga cgtagccgat gcggtgcccc tgcacgatgt ctccttggtt gaaggcggct
     4741 taagtgcact ttctgttccg ttgtgcctca aagcccattt ctgtcaggct gaaatctata
     4801 accttcgcgg gcatgtgtca aaaaatggga aagcagactc tattctgacg aagcggcgcg
     4861 gccctgcctg acatcaagtt agggtatagc ctagattgac atgcgcgatg caacccttaa
     4921 cttgcttgca cctatcgttt ccatgctagc tttatcgtaa cgctaaggaa gctctgaaaa
     4981 agcccatgtt tcgcgaaatc accgctctgt aacccgcatg gttgctggga tgcatttctc
     5041 gagcgggggc tttttcagac cttccttagc ttagcgtacg attttttccg aattctgcgg
     5101 ttcccccatc tgcagctcac agctcaacgg atagacacct tacgcccagg gcatcacaga
     5161 acttggccag gctgtccagc gagccccaga tgcgtacggg ttcgcggcgg ctgcgcacgt
     5221 cgagccagcg cgtggccagc cgggcctgca gctgccagcc gggcgccctg cggatcgccc
     5281 gtagctcacg cagcgcgccg gcttcctgca gcgcctgcag ggcgtcgtga tcgagcgtcg
     5341 ttttcatcgg gcctcctggg cctgtcggtc gtcgccgcaa accatagcag gccctgcagg
     5401 cctaccagag gcccgccctg tggcagcctg caggccctgg agcgggcgcc gtaggccgtt
     5461 aggccgtggg gggctatttg acgtaatcgc aaattacgcg aagtgggagg ggccgctgcc
     5521 tataccgttc gtctggtttt agggggccgc gaggggggct agcacctcct ctacggcccc
     5581 ctttgcattc ccctggagca cgacgagcac atcggaggac agggatgacg agatgactag
     5641 gagcacgcag gtaacctatt aaggttttga tttaaaagag attatatcga tttccgtgcg
     5701 tgaaaccgcg cattacatat tgctgagttg tattattttc agcttattca ggtggtttaa
     5761 tatgacaatt caatccaaaa cataggctat agggatgcgc gaatatagta attgaggttg
     5821 ttgggttttt ggccgagact cacctcaaca gatagcggga acacagttat ggcaaacgga
     5881 aagcaggcct cggcgaaaaa gctaaaaatg gccgagaagg cccaacagtt gatgggtgtc
     5941 cacttccctg gaatccctcc ccagtgggtt tggcatcgga agactaatga tggctattcg
     6001 actgttccac ggacgctgcc gatcgctatg caggcaatcg atacgcaatc taaaggtcaa
     6061 cctgctggcc acacattgct ctgcctgtgg gcgcgagctc cagatcaccc ggtaatcacc
     6121 attgagaacc caaccacatt tgctgctgaa gcgggattcc tgggggaaag ggccgtcgat
     6181 acctggcgcc gccggatgag gacactacgt gatctccact tcatccagac caagccgggc
     6241 gcttcaggcg agttccatta cgtcctgctt atgaatccga acgccgcagt tgaacgaatg
     6301 agggtttctg gtcaggtttc ggatggagtt tacggtcggt tcattgaccg cttgattgag
     6361 gtgggggctt tcggagagat cgaggcaatt cgagagcttt gggaagccca gcgcgccgct
     6421 gctcctgctg tagcagaggc cgctccttcc acgtcctgag ccgagaggag gttcaaaatg
     6481 ctcataagaa gagtttccgg acatctggtg cgggcttgcc caccaggtgt ccaagcggga
     6541 aggaaggccc atagctagag cgggtttcga atcgacctag ctagggccat ggcaagcgca
     6601 atcactcgct gctgagaatg gctgcaacca gactcagcgc ccctagtgta gcaatggaga
     6661 aggactgctt tgaaagcagt ccttctcttt tagggggctg gctcgacatc tgtttatgtc
     6721 acccctgtag agcttgctgc ttggggtggg tgacacgaac ctgcgagctg gacgctacag
     6781 gagacaaggc cgtgacaaaa aaacctcaaa aaaaacccca tttggggcta tttccggtca
     6841 gcttcgctaa atacatggtc tagaaggaaa cagagaccat gaataaccca accaagcaat
     6901 tcctcgtcac cactgcacta tccatccttg ccttgggctt cgcctcaggg cttatccccc
     6961 agtacaaaga cgacggagcg aaacccgagc caacccccta tgtttggtgt gtcccccagc
     7021 caccgacgaa gacctgtctg atccgggtgt gacccccgga tcagacaggt cgaaagtggc
     7081 gcgatgcgcg caggctgcgg taggccgtcc gcgttttgat cctccggaat gctgggtgaa
     7141 tccggatgag ttccgatgat cagaaatgag taaaaacctt tgaaaaatct tttctcattc
     7201 cctaacatgg gaactgagag cgcgaaaccg gttgcctcca ggtggcaata accggcgtag
     7261 gaaccccgta agtagcctgg atactcacgt agggtgagac ctccccatcc ccgcctgtgg
     7321 atgccaatag caggccgtga tccctaaaaa aggggcagct caaagctgcc cctttttctt
     7381 tttctcaggc cttattttct ggcgggctac gctggtagat cggcaggcca tgacgatgct
     7441 tgttcaatga cttcgcgtcg gtgtgcatga aattccacag cacctggcca tcaatcgtat
     7501 cgacgataac cagcatctgc cgtttccctt cgaatgctcc aatgaaggcc aagcggtgat
     7561 tgccattgtc gtactcaacc ttccagacct catatgggtc gtttaacgtg gaaaccgcgt
     7621 ggtttgtgta gcgctcgcga gcatccggtc gcttttccac gatgtgtacc agtttgtccc
     7681 taaccactgt gagcagacca acaggcgtgg tcatggcggt gcgatctatg gtcgggtcac
     7741 ccaggttaaa gacacctgcg acaagctcta cggcagcgtc aacgctgtcg gctgcaggta
     7801 tttcaacagg tgcttgtgag cgaagttcag tcggcaggga acgcaggtcg gctagtccgt
     7861 aatcgaccca ggttttctgg ccaggcaact ccttcacatg gccaggggca ttcatgtagg
     7921 ggcattcctt tcgagtagtg gcgcgcagaa tacctgatcg gtagctatgt gccacgtcca
     7981 ccccaaaggg cgctgcccct tggaaccccg ctcttgccgc cgagggctcg ggagcgtgga
     8041 cggtagagcc gcccacactc ctccgctgag gctattccgt cgaggtttag tcaagggtgc
     8101 gcttcgcccg tgcctccgtt cgccgggccg gtggggctgg cccgacgagc cgggggcgcg
     8161 gcccttgacc tgggtgagct tggaggagat acgtaacccc taacctatta gggaggccca
     8221 acgccgcgcg agccgaggat ttcttatgaa ttcataattc attattgtta ataacctgct
     8281 tgttctagca gctcgcgaat gatcccggaa acggttttct ggcctgagcc cgcccgtttg
     8341 ttctggtggg cggcatcctg taggcgctcg atcatatctg gcggcaggct gatgcctacc
     8401 gcgaccgctt ttctattggt ttcgggagcg attggcggga catctttcct tttcccatag
     8461 ggcttgtctg cgagttcctg tgccaggcgc tcggcctgct ctgtggtggc ggtctggtgg
     8521 cttcctgcag cggtagcaga gcgcggttta accacggtag acctcccgca ccagggcatc
     8581 gatttcgctc gctgcctcgc cattgctggt ttccagcaca gagcgtccct ctgacgcggc
     8641 gtctttataa gctttgcggt agtagcccac tgcatcgagc aattcgaact cggggaattc
     8701 ggctaggtag ccggcgaact cggcgcgctc gcggcccttc accgatggat tggttgaggc
     8761 cattgcgtga tagaccagta cccgcaaatc cgggtttagg tcacggattt tggcgacctg
     8821 ttcctgtagc tcttccaggg tgtcgagatc gaactggctg cactgatggg gggccaggag
     8881 cacgtcagcg acgacgcagc cggtgatcag ctcgcgagag ttgcggccgg ccacgtccac
     8941 gaccacatgc tcgaatttct cggccagggc gcgtatagcg gacgtgatgt tgtcccgctt
     9001 ctcgattagc ggaaacgctg gagagattcc tgacgcttcg cgctccgcgt gccatttggc
     9061 agcggtgcgc tgcgggtcag cgtcaaccag aaccacgtca cggcctgcct tggcgagcgc
     9121 tacagcgata ttcgtggcgg tcgtcgtttt cgacgcgccc cctttattgc agccgacaat
     9181 ggtgatcacc tggcttgctc cttgtgaatc aatatttgct aaatatgaat tatgaattca
     9241 tatttagcaa gattctgccc cttcttggag cccctcacgg agaaggggag ggagctaagc
     9301 cccccgaagg ccagtcttcg ggaggtcgcc tattcggccc gaatgggccg gctacttcag
     9361 ttcgaagtcg tcggataggt tgtgggtctt gtccctctcg gggacatctc caaggccgag
     9421 cagcttttcc acaagtgcca cgatgcagcg caacagccgc tggaaatctc tctcctcgac
     9481 gagctgaccg ttcgcgacct ggccccctgg tagatcgacg tcgaacggca tcgccgggga
     9541 ctggtggcac agccgcctga acacgtcgcg atgttcctcg ctgggcaact cggcatcttt
     9601 caggatgtag tagcggcccc tcaggtgcag cgaatcggcg cgctcttcta cgtccagtcc
     9661 accgccctgg cgtggatgaa ttaagccgcg tctcaagtca tccatcagcg ccgacctaac
     9721 caagttatcg aactcggaac catccccaaa ttggtaaagt tcatccagat ttttcagcaa
     9781 atctaactct tcttttgaag gtctaagaag tttgtcggtt gtaactttca taatatatac
     9841 ctccgagggt ggtttttatg ttattgccct tcatttatat atttgacagt gagcttgctc
     9901 atttgcaagc ataaaatcgt gtggagccag ggctaactcg ggtgccccga gtttctcatt
     9961 tctcttcgct cattcggcct ggcttttcgc tgcgctcaaa gaagaataac aaaagcatga
    10021 gcaaaaaaag gagggggccg cagccgctgc cccaggaaga taaaagagtt attcgcgtgg
    10081 tcatgaagtt gaccgccgaa gaatacgaac tagccaagat taaggccgct ggcattaacc
    10141 tggctaaata cgccagggcg gcattattgc aggcgaaacc agccccgcca ccccctgtat
    10201 tacctacggc aaacgtcgaa gtttggaagg gttctgcagc tctgcagaac aacctcaacc
    10261 aactggttaa acgcctacac gtccaggggc tcgaggccgg cgacgtggaa gaactggccg
    10321 ccttggtggc gagcctacgg gccgcgctgg tgggggttaa atcgtgaaag gcatgcagaa
    10381 gattaagcgc ggcagcggct ttagaggtgt cgccgcgtac atgctcaacg acaaaaacgc
    10441 gcagttactc ggcggaaata tgagtggtcg aaccgccgag gaactgggcc gggagttcaa
    10501 cgtctcccgc cagttgcggc ccgatatcaa gcggccggtt tggcacaact cgctgagact
    10561 gccatcgggc gaacgcctgg ccccggagca gtggtgccag gtggcggacg aatacatgcg
    10621 gcgcatgggg ttcagtgagg cgcatcaacg tgtgtacgtg atgcacgacg accctcaggg
    10681 gcagcatgtc cacatcgcgg caagccgcgt cgcccttgac gggcaggttt atctggggcg
    10741 taacgagaat ctgctcagta cccgccacat tcaggccctg gagcacgatt tcggcctaac
    10801 agtcacccct ggcccgactc tggaaaacgg gcgaatccga gcgccggata tccgccgccc
    10861 aacagccggc gaggtgggcc gctacgaacg taccggagag tctcctgcgc gctatcagct
    10921 ggccggcctg atcgatcagg ccctgcagga caggccgacc gccgtgcagt tcgtggagcg
    10981 cctgcagctc gccggagttg aggttaggcc aaacataaat aaggctggtc taagtggctt
    11041 tagtttctct attaatggcg tggcatttaa gggatcacaa ctaggagata aatataaggc
    11101 gaaagcgcta ataagcaggg ggcttaatta tgagcaagat cgagactatg agtggcttaa
    11161 acgcctcgct gcatcaattg gaaattgtca aagcagcagg ggaaatgcag cagacaatgc
    11221 aggccctgga gatgacacta tccgaggaac gtctgcgcca ggtgctagcg atagttgcga
    11281 gagagtcggc gaagctgttc tccatggaaa tacgacaaac actaaccgac gtgatagcga
    11341 cgcaggagaa gttgatagaa gccattggcg aggcgagagc attaatagaa gccattccga
    11401 aacctccggc ggtacagaag cggaaaggaa cgccattcaa cttgccgaaa cgagtcgtcg
    11461 ccctggaggg caaaataagg gccctggaaa cgctagtaat catcagcata tgcatagcag
    11521 tcgtaagcgt gccggcgccc ctaatactgc tctggaggct tctctaaagt gtcaggaagc
    11581 agcgacgacc agggcggcag atcggatgct gtcactgatg gcaacagcta ttagaaaggc
    11641 cctgcggggt ggggcgcctg gcgagctgac atatggtttg atccagtttt cccggcagtt
    11701 cctggagcag gcgaaaacac cccgccgcga gcccgtccag gagaggcagc cccaggtcga
    11761 ccagcagccg gcgccggagg cgctgcaggg gccccaggag gcccctcgcc aggtgttgga
    11821 aggtcttgct cttgatcatg ctctgcaggg cctggagcag gcccctagcc gcatcgagca
    11881 gcaccgccag gccctagccg agcgcgagcg cgcagagatc aaggccgcgc agcggaagca
    11941 gcgagagaga gaggcgggag tgctggaggg gccggaactg agccttcgct agcagagcgg
    12001 tcgagggatt tggtataaat ctcggattag cccttttcga cgttgccgcc tttgggtacg
    12061 gagccgaacg gaagacgaag gggctagaaa agcaaaaccc cgcctggcag cggggtttta
    12121 caagaagaat gtccagggcc taagcgcgat tcaaacttgg cggtgagagc gcgcagaggc
    12181 ccccttagac ggggatcagt atatgagtag catactcata ccggcaagac cttcccgtcg
    12241 ctcaatgggg cggcgggtat gcatcagcta gaacttttct cacctcagac gctgccgcgt
    12301 cgaccttact gcactgacga tccagcgtcg ggg
//
