LOCUS       NZ_CP077980             2953 bp    DNA     circular CON 13-OCT-2023
DEFINITION  Pseudomonas aeruginosa strain ZPPH29 plasmid p3, complete sequence.
ACCESSION   NZ_CP077980
VERSION     NZ_CP077980.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19950574
            Assembly: GCF_019857425.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Zhu,Y., Chen,J., Shen,H., Chen,Z., Yang,Q.-w., Zhu,J., Li,X.,
            Yang,Q., Zhao,F., Ji,J., Cai,H., Li,Y., Zhang,L., Leptihn,S., Hua,X.
            and Yu,Y.
  TITLE     Deciphering plasmidome of KPC-producing Pseudomonas aeruginosa
            reveals the blaKPC-2 copy number correlates with
            ceftazidime-avibactam resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Zhu,Y., Chen,J., Shen,H., Chen,Z., Yang,Q.-w., Zhu,J., Li,X.,
            Yang,Q., Zhao,F., Ji,J., Cai,H., Li,Y., Zhang,L., Leptihn,S., Hua,X.
            and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, Zhejiang University, 3 East Qingchun Road, Hangzhou,
            Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 138x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/13/2023 02:51:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,722
            CDSs (total)                      :: 6,641
            Genes (coding)                    :: 6,592
            CDSs (with protein)               :: 6,592
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 33 of 49
            Pseudo Genes (internal stop)      :: 7 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077980.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="ZPPH29"
                     /host="Homo sapiens"
                     /db_xref="taxon:287"
                     /plasmid="p3"
                     /geo_loc_name="China:Hangzhou"
                     /lat_lon="30.28 N 120.21 E"
                     /collection_date="2017-04-27"
     gene            complement(join(2144..2953,1..165))
                     /gene="mobV"
                     /locus_tag="KUU70_RS33540"
                     /old_locus_tag="KUU70_33540"
     CDS             complement(join(2144..2953,1..165))
                     /gene="mobV"
                     /locus_tag="KUU70_RS33540"
                     /old_locus_tag="KUU70_33540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_033995413.1"
                     /translation="MAAYAIMRCKKLATMGSVAAALQHCYRERETPNADAERTPDNEHR
                     AATSTDAAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWAKATPQQQAAFFDQAQGWLA
                     AKYGADRIVTASIHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRDKMRADQTSFAEAVR
                     DLGLERGIEGSRATHQRVQSYYGAIERQPGHATITPQAIEPRVLRKGIFSKDVETPEAV
                     AARVTAAVREGYGPTVAAAAGARQEREKARQAQETARSLRDRLKPVLDALGPLNRDMQA
                     KAAQIIKAVGEKLLAEQREATRQRQAQRLRSRGRDRDDGGLSR"
                     /name="mobV"
     gene            1219..1770
                     /locus_tag="KUU70_RS33545"
                     /old_locus_tag="KUU70_33545"
     CDS             1219..1770
                     /locus_tag="KUU70_RS33545"
                     /old_locus_tag="KUU70_33545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA plasmid replication protein"
                     /protein_id="WP_033995415.1"
                     /translation="MQQITVATDAQRRIRQRELAMLDEKEAQEREEARKNKGFTQVYPL
                     GWKRIIELAKGNAGAFGLYSFFAENIDPTCGAVVCDQQFLADKMNVNRRTISRWLSYLE
                     ENRALVRIPVAGKVCAYALDPHEVWKGYDNAKDYAAFVTKTLVNKDGDIRRRIMSMFSG
                     EKQNDDRDPNTIDMFESTPQ"
                     /name="RepA plasmid replication protein"
     gene            1767..2117
                     /locus_tag="KUU70_RS33550"
                     /old_locus_tag="KUU70_33550"
     CDS             1767..2117
                     /locus_tag="KUU70_RS33550"
                     /old_locus_tag="KUU70_33550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="WP_077265610.1"
                     /translation="MSNETKEKIFMKKTFALAAMMAAIAPNLASAADEMIQTKYACEGG
                     KTLDVVYVGDYAVMLQMDELVPLKRAKSASGVRYISASKNYTYELWGKGNDMNLSTHDG
                     GKEQTVLSSCKP"
                     /name="MliC family protein"
ORIGIN
        1 ccccatcgcc gcatcggttg aggtggccgc ccggtgctcg ttgtccggcg tgcgttcggc
       61 gtcagcgttg ggcgtttcgc gctcgcggta gcagtgctgc aacgccgccg ccacgctgcc
      121 catcgtcgcc agcttcttgc atcgcatgat cgcgtaggcc gccatgcctg cccctcccct
      181 tggtgtccga atccggccag tcggggcagc gcaaggcggt gcctccggcg ggccactatc
      241 agatgatagt gtactcacta gactttgcga agcaaagtcg tgcccgcctg cggcggttgc
      301 agcgcccttc gggcttgctc ctgcggcggc cttcggcctt gcccaaaggg gctttgcccc
      361 tctggactcc ccaccctcgc cgccgggagg ctcggggggc aggggtggcg ggcttcgccc
      421 ttcgtccctg cccccactcg cacaggctgg ggcgctgcgg tcagcgtcaa gggttcggct
      481 tcgcccgcgt cctcgcccgg tccctgcgct tcgctccggg ctgcggcctg cgggcgcgcc
      541 cttgaccctg ccctccgctg aaggcgtcag ccagtggccc gctggcgtgt cggaaatcgc
      601 ctcatggccg ggtaggaaat ttccgacagc gccggagtgg aaggcgggtc aagggcgcag
      661 ggggtggctg gcgcggcccg cagcccgcag ggcgaggaca cgggccaccc ccggagagcg
      721 cagcggccct tgacgcgcct ggaacgaccc cacgctcccc ggtaggggtg caggagtgaa
      781 gggccgcagg cccgtagccc ctgcacccct gccacgtggc gaacgcgggg ggtggcgggg
      841 ggcagcgccc accgccccaa ggctgcgcag cggccgccgg aggcccccca aacaacgtca
      901 cccggccggt ggccgggtgg gccttttcca gaggccccgc aggggccgtc agcggggtgc
      961 caaggccgcg tagcggccgc cggcggggcg gcagcccctt agaggagaaa gggggaaatg
     1021 ctaagttatt gatttataag gatttgtaga aatgcattga gacacatagg gtgtcctaac
     1081 gttgagacat gcatggatgt ctcaacaggt agcaaggttg agacgcaaac tgtctcgacg
     1141 tgagacataa tttgtcgtat cattgcgact cgaaatggcg caatgagcgg acaaatttcc
     1201 cgactttgga ggaaccccat gcagcagatc acggtggcga ccgacgccca gcgtcgtatc
     1261 cgccagcgag agctggccat gctggacgag aaagaggctc aggagcgcga agaggcgcgg
     1321 aagaacaagg ggttcacgca ggtgtatccc ttgggttgga agcgcatcat tgagcttgct
     1381 aaaggcaatg ctggggcctt cggcctgtat tcattctttg ctgaaaatat cgacccaacc
     1441 tgcggcgcgg tggtctgcga tcagcagttt ttggcagaca agatgaacgt caacaggcgg
     1501 acaatcagcc gttggttgtc ttacctggaa gaaaatcgcg cgcttgtccg catccctgtt
     1561 gctggcaagg tttgcgccta tgcgctggat cctcacgagg tctggaaggg ctacgacaac
     1621 gccaaggatt acgcagcttt cgtgaccaaa accttggtca acaaggatgg cgatatccgc
     1681 cgccggatca tgtcgatgtt cagcggcgag aagcagaacg acgaccgcga cccgaacacc
     1741 attgacatgt ttgaaagcac tccccaatga gcaacgaaac gaaggagaaa atctttatga
     1801 aaaaaacgtt cgctcttgct gccatgatgg cggcaatcgc tccgaatctt gcttcagcag
     1861 cggatgagat gatccagacc aagtacgctt gcgaaggcgg aaagacgttg gatgtcgtgt
     1921 acgtcggaga ctatgccgtg atgctgcaaa tggatgagct tgtcccactg aaacgcgcca
     1981 agagcgcatc aggagtgcgc tacatctccg ctagcaaaaa ctacacctac gaactgtggg
     2041 gtaagggcaa cgacatgaac ctttcgaccc acgatggcgg gaaggagcaa accgtcctca
     2101 gtagctgcaa gccataaacc ccttccgtcg gccacctgta gggtcaacga gacagcccgc
     2161 cgtcgtcgcg atcccggccg cgactgcgaa gccgttgggc ctgccgctgg cgtgtcgcct
     2221 cgcgctgttc ggcgagcagc ttctcgccca cggccttgat gatctgggcc gccttggcct
     2281 gcatatcgcg attaagcggc cccagagcgt ccagaacggg cttcaggcgg tcacgcaggc
     2341 tccgggctgt ctcctgtgcc tgccgggcct tctcgcgctc ctgacgcgct cctgcggccg
     2401 ctgcgacggt cgggccatac ccttccctga cggcagcggt cacgcgggcg gccacggcct
     2461 ccggcgtctc cacgtccttc gagaaaatcc ccttgcgcag cacccgcggc tcgatggcct
     2521 gcggcgtgat ggtggcgtgc ccgggctggc gctcgatggc gccgtagtag ctctggacgc
     2581 gctgatgggt ggcccggctg ccctcgatgc cgcgttccag cccgagatcc ctcaccgcct
     2641 cggcaaagct ggtctggtcg gcccgcatct tgtcgcggcc accgatgaac tccttggccg
     2701 acagccgccc gtcctgcgtc agcggcacga cgaaggccga caggtgcggg gtggcctcgt
     2761 cgcggtggat gctggccgtc acgatgcgat ccgcgccgta tttggccgcc agccagccct
     2821 gcgcctggtc gaagaacgcg gcctgctgct ggggcgtggc cttggcccac cattccgggc
     2881 tggccgtcat cacgtattcg accgccagca ccgcgtcctt gcgccgcttc tccggcagca
     2941 actcgcgcag ccg
//
