LOCUS       NZ_CP077976             2953 bp    DNA     circular CON 13-OCT-2023
DEFINITION  Pseudomonas aeruginosa strain ZPPH33 plasmid p5, complete sequence.
ACCESSION   NZ_CP077976
VERSION     NZ_CP077976.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19950575
            Assembly: GCF_019857405.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Zhu,Y., Chen,J., Shen,H., Chen,Z., Yang,Q.-w., Zhu,J., Li,X.,
            Yang,Q., Zhao,F., Ji,J., Cai,H., Li,Y., Zhang,L., Leptihn,S., Hua,X.
            and Yu,Y.
  TITLE     Deciphering plasmidome of KPC-producing Pseudomonas aeruginosa
            reveals the blaKPC-2 copy number correlates with
            ceftazidime-avibactam resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Zhu,Y., Chen,J., Shen,H., Chen,Z., Yang,Q.-w., Zhu,J., Li,X.,
            Yang,Q., Zhao,F., Ji,J., Cai,H., Li,Y., Zhang,L., Leptihn,S., Hua,X.
            and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, Zhejiang University, 3 East Qingchun Road, Hangzhou,
            Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 117x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/13/2023 03:00:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,445
            CDSs (total)                      :: 6,365
            Genes (coding)                    :: 6,306
            CDSs (with protein)               :: 6,306
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 25 of 59
            Pseudo Genes (incomplete)         :: 41 of 59
            Pseudo Genes (internal stop)      :: 4 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077976.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="ZPPH33"
                     /host="Homo sapiens"
                     /db_xref="taxon:287"
                     /plasmid="p5"
                     /geo_loc_name="China:Hangzhou"
                     /lat_lon="30.28 N 120.22 E"
                     /collection_date="2017-03-14"
     gene            join(2805..2953,1..403)
                     /locus_tag="KUU78_RS32110"
                     /old_locus_tag="KUU78_32095"
     CDS             join(2805..2953,1..403)
                     /locus_tag="KUU78_RS32110"
                     /old_locus_tag="KUU78_32095"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA plasmid replication protein"
                     /protein_id="WP_033995415.1"
                     /translation="MQQITVATDAQRRIRQRELAMLDEKEAQEREEARKNKGFTQVYPL
                     GWKRIIELAKGNAGAFGLYSFFAENIDPTCGAVVCDQQFLADKMNVNRRTISRWLSYLE
                     ENRALVRIPVAGKVCAYALDPHEVWKGYDNAKDYAAFVTKTLVNKDGDIRRRIMSMFSG
                     EKQNDDRDPNTIDMFESTPQ"
                     /name="RepA plasmid replication protein"
     gene            400..750
                     /locus_tag="KUU78_RS32115"
                     /old_locus_tag="KUU78_32100"
     CDS             400..750
                     /locus_tag="KUU78_RS32115"
                     /old_locus_tag="KUU78_32100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="WP_077265610.1"
                     /translation="MSNETKEKIFMKKTFALAAMMAAIAPNLASAADEMIQTKYACEGG
                     KTLDVVYVGDYAVMLQMDELVPLKRAKSASGVRYISASKNYTYELWGKGNDMNLSTHDG
                     GKEQTVLSSCKP"
                     /name="MliC family protein"
     gene            complement(777..1751)
                     /gene="mobV"
                     /locus_tag="KUU78_RS32120"
                     /old_locus_tag="KUU78_32105"
     CDS             complement(777..1751)
                     /gene="mobV"
                     /locus_tag="KUU78_RS32120"
                     /old_locus_tag="KUU78_32105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_033995413.1"
                     /translation="MAAYAIMRCKKLATMGSVAAALQHCYRERETPNADAERTPDNEHR
                     AATSTDAAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWAKATPQQQAAFFDQAQGWLA
                     AKYGADRIVTASIHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRDKMRADQTSFAEAVR
                     DLGLERGIEGSRATHQRVQSYYGAIERQPGHATITPQAIEPRVLRKGIFSKDVETPEAV
                     AARVTAAVREGYGPTVAAAAGARQEREKARQAQETARSLRDRLKPVLDALGPLNRDMQA
                     KAAQIIKAVGEKLLAEQREATRQRQAQRLRSRGRDRDDGGLSR"
                     /name="mobV"
ORIGIN
        1 cattgagctt gctaaaggca atgctggggc cttcggcctg tattcattct ttgctgaaaa
       61 tatcgaccca acctgcggcg cggtggtctg cgatcagcag tttttggcag acaagatgaa
      121 cgtcaacagg cggacaatca gccgttggtt gtcttacctg gaagaaaatc gcgcgcttgt
      181 ccgcatccct gttgctggca aggtttgcgc ctatgcgctg gatcctcacg aggtctggaa
      241 gggctacgac aacgccaagg attacgcagc tttcgtgacc aaaaccttgg tcaacaagga
      301 tggcgatatc cgccgccgga tcatgtcgat gttcagcggc gagaagcaga acgacgaccg
      361 cgacccgaac accattgaca tgtttgaaag cactccccaa tgagcaacga aacgaaggag
      421 aaaatcttta tgaaaaaaac gttcgctctt gctgccatga tggcggcaat cgctccgaat
      481 cttgcttcag cagcggatga gatgatccag accaagtacg cttgcgaagg cggaaagacg
      541 ttggatgtcg tgtacgtcgg agactatgcc gtgatgctgc aaatggatga gcttgtccca
      601 ctgaaacgcg ccaagagcgc atcaggagtg cgctacatct ccgctagcaa aaactacacc
      661 tacgaactgt ggggtaaggg caacgacatg aacctttcga cccacgatgg cgggaaggag
      721 caaaccgtcc tcagtagctg caagccataa accccttccg tcggccacct gtagggtcaa
      781 cgagacagcc cgccgtcgtc gcgatcccgg ccgcgactgc gaagccgttg ggcctgccgc
      841 tggcgtgtcg cctcgcgctg ttcggcgagc agcttctcgc ccacggcctt gatgatctgg
      901 gccgccttgg cctgcatatc gcgattaagc ggccccagag cgtccagaac gggcttcagg
      961 cggtcacgca ggctccgggc tgtctcctgt gcctgccggg ccttctcgcg ctcctgacgc
     1021 gctcctgcgg ccgctgcgac ggtcgggcca tacccttccc tgacggcagc ggtcacgcgg
     1081 gcggccacgg cctccggcgt ctccacgtcc ttcgagaaaa tccccttgcg cagcacccgc
     1141 ggctcgatgg cctgcggcgt gatggtggcg tgcccgggct ggcgctcgat ggcgccgtag
     1201 tagctctgga cgcgctgatg ggtggcccgg ctgccctcga tgccgcgttc cagcccgaga
     1261 tccctcaccg cctcggcaaa gctggtctgg tcggcccgca tcttgtcgcg gccaccgatg
     1321 aactccttgg ccgacagccg cccgtcctgc gtcagcggca cgacgaaggc cgacaggtgc
     1381 ggggtggcct cgtcgcggtg gatgctggcc gtcacgatgc gatccgcgcc gtatttggcc
     1441 gccagccagc cctgcgcctg gtcgaagaac gcggcctgct gctggggcgt ggccttggcc
     1501 caccattccg ggctggccgt catcacgtat tcgaccgcca gcaccgcgtc cttgcgccgc
     1561 ttctccggca gcaactcgcg cagccgcccc atcgccgcat cggttgaggt ggccgcccgg
     1621 tgctcgttgt ccggcgtgcg ttcggcgtca gcgttgggcg tttcgcgctc gcggtagcag
     1681 tgctgcaacg ccgccgccac gctgcccatc gtcgccagct tcttgcatcg catgatcgcg
     1741 taggccgcca tgcctgcccc tccccttggt gtccgaatcc ggccagtcgg ggcagcgcaa
     1801 ggcggtgcct ccggcgggcc actatcagat gatagtgtac tcactagact ttgcgaagca
     1861 aagtcgtgcc cgcctgcggc ggttgcagcg cccttcgggc ttgctcctgc ggcggccttc
     1921 ggccttgccc aaaggggctt tgcccctctg gactccccac cctcgccgcc gggaggctcg
     1981 gggggcaggg gtggcgggct tcgcccttcg tccctgcccc cactcgcaca ggctggggcg
     2041 ctgcggtcag cgtcaagggt tcggcttcgc ccgcgtcctc gcccggtccc tgcgcttcgc
     2101 tccgggctgc ggcctgcggg cgcgcccttg accctgccct ccgctgaagg cgtcagccag
     2161 tggcccgctg gcgtgtcgga aatcgcctca tggccgggta ggaaatttcc gacagcgccg
     2221 gagtggaagg cgggtcaagg gcgcaggggg tggctggcgc ggcccgcagc ccgcagggcg
     2281 aggacacggg ccacccccgg agagcgcagc ggcccttgac gcgcctggaa cgaccccacg
     2341 ctccccggta ggggtgcagg agtgaagggc cgcaggcccg tagcccctgc acccctgcca
     2401 cgtggcgaac gcggggggtg gcggggggca gcgcccaccg ccccaaggct gcgcagcggc
     2461 cgccggaggc cccccaaaca acgtcacccg gccggtggcc gggtgggcct tttccagagg
     2521 ccccgcaggg gccgtcagcg gggtgccaag gccgctacgc ggccgccggc ggggcggcag
     2581 ccccttagag gagaaagggg gaaatgctaa gttattgatt tataaggatt tgtagaaatg
     2641 cattgagaca catagggtgt cctaacgttg agacatgcat ggatgtctca acaggtagca
     2701 aggttgagac gcaaactgtc tcgacgtgag acataatttg tcgtatcatt gcgactcgaa
     2761 atggcgcaat gagcggacaa atttcccgac tttggaggaa ccccatgcag cagatcacgg
     2821 tggcgaccga cgcccagcgt cgtatccgcc agcgagagct ggccatgctg gacgagaaag
     2881 aggctcagga gcgcgaagag gcgcggaaga acaaggggtt cacgcaggtg tatcccttgg
     2941 gttggaagcg cat
//
