LOCUS       NZ_CP077845            11194 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Acinetobacter baumannii strain DETAB-E155 plasmid pDETAB9a, complete
            sequence.
ACCESSION   NZ_CP077845
VERSION     NZ_CP077845.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19762632
            Assembly: GCF_023517715.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Liu,H., Moran,R.A., Doughty,E.L., Hua,X., Ba,X., Jiang,Y.,
            Holmes,M., Mcnally,A., Van Schaik,W. and Yu,Y.
  TITLE     DETECTIVE: Carbapenem-resistant Acinetobacter baumannii study 2019
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11194)
  AUTHORS   Liu,H., Moran,R.A., Doughty,E.L., Hua,X., Ba,X., Jiang,Y.,
            Holmes,M., Mcnally,A., Van Schaik,W. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2021) Department of Infectious Diseases, Zhejiang
            University School of Medicine, Qingchundong 3 RD, Hangzhou 310016,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023517715.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 04:46:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,795
            Genes (coding)                    :: 3,727
            CDSs (with protein)               :: 3,727
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 23 of 68
            Pseudo Genes (incomplete)         :: 35 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077845.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="DETAB-E155"
                     /isolation_source="Infusion stand_P63_BU16"
                     /db_xref="taxon:470"
                     /plasmid="pDETAB9a"
                     /geo_loc_name="China: Hangzhou, Sir Run Run Shaw Hospital
                     ICU"
                     /lat_lon="30.256965 N 120.201625 E"
                     /collection_date="2019-09-03"
                     /collected_by="Yunsong Yu"
     gene            1..936
                     /gene="repM"
                     /locus_tag="KU039_RS19410"
                     /old_locus_tag="KU039_19315"
     CDS             1..936
                     /gene="repM"
                     /locus_tag="KU039_RS19410"
                     /old_locus_tag="KU039_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001292329.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLREASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YESQSGIIFLRFTQDIVPLITRLEENFTKYELQQVSRLSSSYAIRLYELLIQWRSAGKT
                     PLFDLSIFRQQLGVKPHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFTFKQKKMSNLPIKNKRDPDTIDIFSKMTDAQRHLFSHKLSELPEMGKYSHGTESYPQ
                     FAVRIAEMLQNPEKFKELYPYLQKVGFKAA"
                     /name="repM"
     gene            1014..1532
                     /locus_tag="KU039_RS19415"
                     /old_locus_tag="KU039_19320"
     CDS             1014..1532
                     /locus_tag="KU039_RS19415"
                     /old_locus_tag="KU039_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000839337.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPVQKQSVKETVKVDSPTSAEVLLLRQQIDLLKNQLDDAKNRESFYQNQIETMQRLL
                     EAPKTNTTQSDDPGSTQAESTVEEKEDVIAMPPKESEVIENKRIPVPEHIEPEPEKRGF
                     WSRFFKPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            1742..2041
                     /locus_tag="KU039_RS19420"
                     /old_locus_tag="KU039_19325"
     CDS             1742..2041
                     /locus_tag="KU039_RS19420"
                     /old_locus_tag="KU039_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            2309..2596
                     /locus_tag="KU039_RS19425"
                     /old_locus_tag="KU039_19330"
     CDS             2309..2596
                     /locus_tag="KU039_RS19425"
                     /old_locus_tag="KU039_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438827.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            2583..2897
                     /locus_tag="KU039_RS19430"
                     /old_locus_tag="KU039_19335"
     CDS             2583..2897
                     /locus_tag="KU039_RS19430"
                     /old_locus_tag="KU039_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            3025..5436
                     /gene="znuD2"
                     /locus_tag="KU039_RS19435"
                     /old_locus_tag="KU039_19340"
     CDS             3025..5436
                     /gene="znuD2"
                     /locus_tag="KU039_RS19435"
                     /old_locus_tag="KU039_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            5741..6202
                     /locus_tag="KU039_RS19440"
                     /old_locus_tag="KU039_19345"
     CDS             5741..6202
                     /locus_tag="KU039_RS19440"
                     /old_locus_tag="KU039_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(6528..6737)
                     /locus_tag="KU039_RS19445"
                     /old_locus_tag="KU039_19350"
     CDS             complement(6528..6737)
                     /locus_tag="KU039_RS19445"
                     /old_locus_tag="KU039_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069474.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(6730..7032)
                     /locus_tag="KU039_RS19450"
                     /old_locus_tag="KU039_19355"
     CDS             complement(6730..7032)
                     /locus_tag="KU039_RS19450"
                     /old_locus_tag="KU039_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7025..7381)
                     /locus_tag="KU039_RS19455"
                     /old_locus_tag="KU039_19360"
     CDS             complement(7025..7381)
                     /locus_tag="KU039_RS19455"
                     /old_locus_tag="KU039_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7947..8246)
                     /locus_tag="KU039_RS19460"
                     /old_locus_tag="KU039_19365"
     CDS             complement(7947..8246)
                     /locus_tag="KU039_RS19460"
                     /old_locus_tag="KU039_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            complement(8363..9124)
                     /locus_tag="KU039_RS19465"
                     /old_locus_tag="KU039_19370"
     CDS             complement(8363..9124)
                     /locus_tag="KU039_RS19465"
                     /old_locus_tag="KU039_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            9429..10598
                     /locus_tag="KU039_RS19470"
                     /old_locus_tag="KU039_19375"
     CDS             9429..10598
                     /locus_tag="KU039_RS19470"
                     /old_locus_tag="KU039_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002134206.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFEVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     Typing          2..936
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9508..10595
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagtgaat taatcgtaaa ggataatgct ctaattcaag caagttatac cttagataca
       61 gttgaacaaa gactgatcct attagccatt gctgaagctc gagaaactgg acatgggata
      121 actgaaaata gtcttttaga agtacatgca agtagttata taaatacttt taatgtcgag
      181 aaacataccg cttataccgt actcagagag gcatctaaaa gcttatttga tcgctatgtc
      241 acataccatg acattaatcc taagacaggt aaggatcgta gctttcattg tcgttgggtc
      301 gacaaaattg ggtatgaatc tcaatcagga attattttcc tacgattcac ccaagatatc
      361 gttccactca taactcgact tgaagaaaat ttcactaaat atgaattgca gcaggtttct
      421 aggttaagta gctcatatgc tattcggcta tacgagctat taattcaatg gagatctgcc
      481 ggaaaaacgc cactttttga tctatctatc tttagacaac aacttggtgt caaacctcat
      541 caatacaaaa caatgagtaa ctttaaaaca tatgttttag attttgctct taagcaggta
      601 aatgagttaa ccgatataac agctaaatat gagcaacata aaaaagggcg ttctatttca
      661 ggtttttcat tcactttcaa acagaaaaaa atgagtaatc tgccaataaa aaataagcgt
      721 gacccagaca ctatagatat tttctcaaaa atgacagatg ctcaacgcca tctgttttcc
      781 cacaaactgt cagaacttcc tgaaatggga aagtattctc atggtacaga aagctatccg
      841 caattcgctg tacgcattgc agaaatgctt caaaacccag aaaagtttaa agaactctat
      901 ccttatctcc aaaaagtagg cttcaaagct gcatagaaga tagacttgct gatttgtcta
      961 ttagacagtc taatagacac taatttttta caagattagg cagagattta cacatgaaaa
     1021 gccttacagt tcttgagctt tctaagcttt acaatattaa tcgacaaacc atttacaaca
     1081 atataaagaa aggtatttta agtaaaaatt ctgacaataa gattgatctt gcagaagcga
     1141 tccgggtatt tggtgagcca gtacaaaaac agagtgtaaa agaaactgta aaagtagaca
     1201 gtccaacttc ggcagaagtt ttactcctta gacagcagat agacctacta aaaaatcagc
     1261 tagatgatgc aaaaaataga gaaagcttct atcaaaatca aattgaaact atgcaacgcc
     1321 tattggaagc tccaaaaacc aatacaacac aatctgatga tcctggatca acacaggcag
     1381 aatccacagt agaagaaaaa gaggacgtca tagctatgcc tcctaaggag tcagaggtta
     1441 tcgagaataa gcgtattcca gtccctgaac atattgagcc agaaccagaa aaaaggggct
     1501 tctggagccg ttttttcaag ccctatggat gataggacta tttaatcaga ctaatcaaaa
     1561 ccgtggaaca atgatcaata attgatcgtg aaacataaaa aatcactaaa acagcctgag
     1621 cgtgactgct taggctgttt ttacatccat aagacattgt tttaaaatgc tttttacaaa
     1681 gaatttcgta taaggtgtat tatgttaatt ttagggaaac ttaattctat aggtgatacc
     1741 aatggacaaa aagatagaag agaaaactaa agatattaat gaaaaaattc aaaaattgga
     1801 tttagctatt caagaactaa tagaaactac agacaatttt tttattaatg ataagtacta
     1861 tggaattgaa tgtgctttta ttttagacca tgcactaaat attaaaatgc actttttcaa
     1921 caagtatcca gcatttgcac cacctcactt acgaggagta gaaaattatg atcgaattat
     1981 acatattaga gaaaataatc ctgaagataa gctagaacaa atttctcctc ttaaagacta
     2041 aattttctaa tcttgtactt agagtcccct actatttaac ataatggata ttatacgaag
     2101 tacacttgta tattaattta aatatcaact acttaataaa aatataaaaa tccaaaaatg
     2161 cccaaaaagc cgactttgaa acaagtcggc ttttttatga tcgattttga tagtttttgc
     2221 caataaatct gactaaaaat tacgcttctc aacttgagtg atacaatttc gtgatacatt
     2281 attgtatata caaatttgaa tattttttat ggaacagtat tttgaatggg atgaggctaa
     2341 gaatcgaaag aatcagaaaa agcatgatat ctctttcgaa actgcaagcc ttgtttttga
     2401 agatccatta cggatctcaa tccaagacag atatactaat ggtgaagaac gttggcaaac
     2461 cattggacga gttaaaggcg tactaatgtt attggtggct cacaccattt ttgatgaaga
     2521 tgactgtgaa atcatccgaa tcattagtgc aaggcaagtc actaaagcgg agcgaaacaa
     2581 atatgagcat ggttagatac tcacacaaag agctgaatga gaaatttggt gagaagcaag
     2641 atgctgaaat tcaacgcttg cttgctaaag gcacggttcc tgatgatcag ctagatctat
     2701 cagatattcc tgaaattaca gactggagca atgctgtacg ccaaaatcaa ttttaccgcc
     2761 cagtaaagca acaaacttca attcggttag atgctgatgt acttgcttgg ttcaaagcac
     2821 aaggcaaagg ttatcaaaca cgtatgaatg aaattttacg agatgctatg ctaaaagaat
     2881 taaaaaatca tcaataagaa tgggagctta ggctcccatt tttgacttca tttttagggg
     2941 ggattaccat tcaaaaattt aaatagtaga ttttataaat ttttaagtgt tatattataa
     3001 tataacatta aatttggttg ttttatgcta aataaaagta aactattttt agctttaatt
     3061 actttagggg cctcgaaaat tttattggca gctgaagggc ctgttactac cttaaataca
     3121 atagtattaa ctgcacaatc tgatgagtta gggtctgagc tgttaggcaa aagtctaaat
     3181 gttagtaatc agtttattga tactagtaaa cttaaacagc gttcgacaac tctaggggat
     3241 gccttgggta cggaattagg aattcattct aatcaatatg gaggtggagc atctactcct
     3301 attattcgtg ggcaggaagg aaaacgtatt aaagttttac agaataatgc tgacgtgttg
     3361 gatatgtcta atatgtctcc agatcacgct gtcacagttg aaccaagttt agctaagtct
     3421 attgaaatta tacgtggtgc aagtaccctt ttgtatagtt caaatagtgc ggcaggggta
     3481 gtcaatgtca ttgactataa aattccaacc caaatgcctc aagacggatt agagggtaat
     3541 actacattac gttttaatac tggcagtaat gagaagttga ccacagcagg tgtaacagtt
     3601 ggcttaagcc cacatgtagc tttacgggca gaagggttat atcgtaatgc tggtaactat
     3661 aaaactcctc attaccagtc tagctcttat aattctttag aagatcttga aaaccaaaac
     3721 agtatttata aaaatttaaa atatttacca gaaagctggg cagagtcgag agtaggtacg
     3781 cttggattat cctggataga cgataatact tatttaggag tttcttatac acatcgtcat
     3841 gatgaatatg gtttaccagc ccatagccat ttatatgaag gttgtggtgc ctctgcaatc
     3901 ggtatcgatt cacgaattag tggattaaaa aattatcttt tatattatcc tcaattgatg
     3961 gatgagcaag atattaatta cataaatcca cgtcctgatt gtcatcagca taatcatatt
     4021 catgaaacta atttctctca taatgctcct tatattgatt taaatactcg acgttatgat
     4081 gtgcgtggag aattcactca accatttacc ggtattgaca aaattcgtac ctcattgagt
     4141 tatatagatt attttcacaa tgaactcgag ggagataaga ttactaactt ttttaaaaat
     4201 acaggaaaag taggccgcat agagttatca catcagccat taggggaatt aaccggtatc
     4261 ttggggctac aatatttgga acaggataat agtgctttat caccagtcca ttctcaagaa
     4321 ggccatacca cctatctaga taatcagcag ctattaaatc gaaatgtcac taaaaacttt
     4381 agtgtatttg gcctagaaaa atataactgg aacgacttta ctttcgaact aggtgctcgt
     4441 atagaaaagc agaaagttag tatggattat gatatagaaa aaattaaaga ttcaatgaaa
     4501 ccttggccta ataaatataa cagtccttat gtagaaaaaa ataataaaat cagggctcaa
     4561 aatttaaaat ctattttaga agctgttcaa cctaacaaag aaacagcttt ttcttatgca
     4621 ggtacagtcc actggagatt tgctccaaat tatatcttat cattaacagg tacacatcaa
     4681 gaaagattac ctaatgcaca agaaatgtat actcatggta tgcatctcgc tactaattct
     4741 tttgaaatag gtaaccgctt tctaagaaaa gagaaatcca ataatttaga aatcagtctg
     4801 gcctataagg atgatttact tgattatcaa atcagtactt actattatga ttttgataac
     4861 tatatatatt tacagacact caatgaagtt ttaggaacta caaaagttag agatcagcat
     4921 acactacgta ttaatcatta tagccagtct gctgctaatt tttatggatt agagggcaac
     4981 atagggtatc aatttaattc tgtttatcac ggctctcttt ttggagatta tgtgaaaggc
     5041 cgtttaacaa atttacctga tgctgtaatt gcatacgata tctggaatag agaaccgact
     5101 ttagctcccc aaaaggatcg ttacactcct agattacctc ctgctcgttt gggtacacga
     5161 ctaaaagctg attttgatga atcgcttaaa ggtgaaatag aatattatcg agtctttaaa
     5221 caagataata tttctaaatt tgagcaggtt acatcaggtt ataatatgct taatatgact
     5281 ttagcatata aaaataagct atctcacaca gaatatgatt tattctttaa agcgaataat
     5341 ttacttgatc agaaagtcta tgcccatgaa acatttttgc catatattcc ccaaattgga
     5401 agaaatttta gtttaggctt aaatcttaat ttctagataa gatactgtaa tcttctaaga
     5461 aaatttctta gaagattttt cttataaaat gattagatta ataattaagg taagcgtccg
     5521 ctgaatccca tacctatttt ttaattcgat ttaggtttcc agcagtgtgg cagtaactct
     5581 tcaatctggg tcactttatg tgtcggcagc cttttcagca ctgaatagcc accgattttg
     5641 tagacacctt ttagctagat aaaaagcaat ttttttgatc tttaaagaat gaggatataa
     5701 gctgtttaaa acttaattct aattggttaa taggtgaaaa atgaaattag cagaagctct
     5761 cttacttcga agtgatcaac aaaaaaaaat tatttcacta aaacaacgta tcaatgcaaa
     5821 tgtattggta caagatggtg atcaaccatc tgaagatcca aatgaactac taaaacaagt
     5881 attttcttta attcaagaat ttcaaaaatt aagttatgca attcatgaaa ctaatgcttt
     5941 aacaaaatta aatgatggcc gttctctact tgctttattg actttgcgtg atgaatttgt
     6001 agaacagcac aaaaccctaa ctgcagcaat aagtaataca tctcgtgaat ctgatcgtta
     6061 tagtacgcgg gaaataaagt ggcataaagt tattccagtt tcatcattac aaaaacaagc
     6121 tgatgatatt agtcttaaat tacgtgattt aaacgtgtta attcagtcga ctaattggaa
     6181 aattgatttg attgaagcct aatagtcctt tagatataat caagttgaac atactgggcg
     6241 agtacaagat cccttacttc cacaacatag gattgtaaat aaagtgtagt ggcttgcaaa
     6301 gcaagtggat aagcttaaaa ttttaagtac aaatcaatcc atcacttggc ataactcaaa
     6361 tctttgtgca aacaaagtta cacgttactt ataactacca tgtactgaca gtatgttcac
     6421 ctcaattact aaaaataaag aaatgagcaa gcatcagctc aactacattt ctatttttaa
     6481 ttcatttcgg catttaacat aaaatctctt atgcgaatct agaaggatta agctatttcg
     6541 taatatttta tccaataagc attgtcaaaa atatcaatgt aatagcttgg cctcttacaa
     6601 tgtgaaaaat attcacagca taaatctcta ataatttcaa tttcttctag gctatcggca
     6661 gcattgacta aaaatataaa cttgtctaag ttaacaagta atttagtact atttttcaaa
     6721 ggtttattat tagacattga accctatgtg ttttcctgtt ggaagctcta catcaatacg
     6781 aagttttcca cccattgctt caacatactt tttcatcgtt gaaattttaa gatcatgacc
     6841 acgattttcg attgctgata atgacggctg tttaattcca agggttgcag ctagttcttt
     6901 ctgggaaatt tctaactctt ctcggataag gtgaagctga ctttctaaaa gtaattcatc
     6961 agccattttt tgaatacgag cttgactctc tggagagcga ctagccaata attcttgtaa
     7021 gctcttagcc attagattga tctcctaaag ttgagagatg aagtgcgtat tgctcatctg
     7081 caattgctag catctctgta taaaaacgct ttttattact cttatcacca atacacagca
     7141 caatcgcttg tcttagggga tcgaatgcaa aaaatgccct taacggttta ccacgatgct
     7201 gaacacgtag ttccttcata ttggtaaatt tagaatcgta gacggtatct actaaaggac
     7261 gacctaaact aggcccttgc tgttgtagaa caaccaatgc agctaagact ttctcttgtg
     7321 ttgcttcatc ttgctgttca agccattcat taaacagatc cgttgtaatg actgtccaca
     7381 tattacaatc acaatataga ttacagtcta tatattaaat ctgattgaat gactttgcaa
     7441 atggcaataa aaaatcccga cgtcatgtcg ggatttttcg gtatggagcg cataggtagg
     7501 gctgactacc tgatggtcat ggtgcctgac tgattattat tgttgtttta agtcattgaa
     7561 catcctgtcc atatagatcg atcataagag cttcatcggg ggatagatac agacaatttc
     7621 gtgaaacgct gtaagccaaa acgtacaaaa aataatcaaa atttatccca gtggttatcc
     7681 acagaaatag tggattcttt tgggtaaaaa agcttataaa ctctatggaa ttgctatttt
     7741 tgattatttt ttaaacaatt ttattggcaa gaagtatcaa aattgagcat aaaaaaaccg
     7801 acttgtttct aagtcggttg aacgaggtcg ttccagaggg tatgactcaa tgtaagctac
     7861 tgatcttaaa gtaatcttaa agatccattt cgcataatat ccattatgtt aaatagtagg
     7921 ggactctaag tacaagatta gaaaatttag tctttaagag gagaaatttg ttctagctta
     7981 tcttcaggat tattttctct aatatgtata attcgatcat aattttctac tcctcgtaag
     8041 tgaggtggtg caaatgctgg atacttgttg aaaaagtgca ttttaatatt tagtgcatgg
     8101 tctaaaataa aagcacattc aattccatag tacttatcat taataaaaaa attgtctgta
     8161 gtttctatta gttcttgaat agctaaatcc aatttttgaa ttttttcatt aatatcttta
     8221 gttttctctt ctatcttttt gtccattggt atcacctata gaattaagtt tccctaaaat
     8281 taacataata caccttatgc gaaattccaa tgtaagccca aaatagaaat gtccaacttc
     8341 tctaaaatcc ctgctaaata aactactctt cgcctttttc taaggccttt ttaatttcaa
     8401 ttactgacaa taattctccg cccttttgcc aggtatgtaa tccttctaac acctgaataa
     8461 aaagtttatt tgctttaaaa tattcatttt catatgtctt aacatcttta aaaattaaag
     8521 atttacgtac ttcattatga aactttgtcg tattcacaat ccgattggta atctgttgag
     8581 gagtatcatt gctaatatca atattgagtt tcttaaatgc gacaagaaca gcaccaccta
     8641 attcaaattt acgttgattt tcagctttgc gttttttccc agcatccttc ttctgtagct
     8701 gactcaaata caattctaaa tcactgtatt caagcttttg cgctttaaaa ttcagatacc
     8761 gtgtcgccaa ggtttttaaa atatttagtt ccatctgatc tagtgcattc ttttgaaaaa
     8821 gttcatacag atctaaaaac ttctgttcat actcggtttt aagaggcaga ttattcaact
     8881 cttcaaacag caatttcaac ttcagatgtt cacgataagg ttttaaagcc tctttgacca
     8941 gaacctgatc tgtctgagtt agcccctcaa aattacaatc aacactattt aaagccttta
     9001 acacttgaga ttgaagatca gaaagctgtt cttgctgttc taaaaactct acaaatgcat
     9061 gtaactcagc aatctgtaac aactgagcag gtcgaccacg cttttttgct ttttccattt
     9121 ccatcatctg ccgccaaatt ttcagaactg caacctcttt aaatatggtt ttaccatatt
     9181 taaagagggc agtctgaaaa taattattta atataaaaat aaaaattctt ttcaagaaat
     9241 tttattttta tattaaataa ttcgcacttg acagtttctc ctcatcggag aaactagtac
     9301 gcaatggggc gcttccccct tgaccccctt caaccggatt tttcagccat acaggcattt
     9361 caaatccggg cggttttcat tccgtttcac tgcataaaaa ccgaacccac aacccagcag
     9421 aggtgaacat ggccagctac cacttcagcg tcaaaagcaa aaataagggc tatgcgaccg
     9481 gccattatct ctacatttcc cggctgatgc agtatgaagg catccgcaaa accagcaatg
     9541 agaccataga acatattgaa cctggtcgat gtatgcctgc ctttgtaaaa gatcccattg
     9601 atttttggca ggcagcagat acgtatgaac gagccaatgc caaggcctac atggagtatg
     9661 aaattgccct accgaatgag ttcacgtcag aacagcgtaa aacgctcata gagacgtttt
     9721 tagacaaaca tatagttcca cagcaatatc cgcactcata cgccatacac aacgtaaaat
     9781 cacgtatcag tggcgaagat cagcctcatt gtcatctgat gttctcgcta aaagcggatg
     9841 acggcattga acgcagcgca gaacagtact tcaaacgcta taaccctcaa gatccagcca
     9901 aaggcggtgc caaaaaaatc caacttcaag atggccatga agactattcc acttttttga
     9961 tctatatccg aaaacaatgg gaaaatcatt tgaatgatgc ccttgcacag cactgcccga
    10021 ccgtcaccta cacactggat ggccaagaca tcaccatcaa aaatcaggtt tcagcagaca
    10081 gctatgaaaa atataacgag atccacagca cgctatacct tccagagcca aaactaggcg
    10141 ttggacaaca aaatgcgaca gcggaatatc tcgcccaaat ccaaaaaatc agagaacaca
    10201 accagcaaga acgagaactc gaagaacgcc aaaagcaatt agatcaacaa catgaatatt
    10261 tttatagtgc tgaaaacaac ccattttcat cattggaagt catgtattac ctgaattact
    10321 taatcaagaa tgcaacaaat aaggacaaac taaaaaatga tttgaatgaa attatcaaaa
    10381 accaaggccc tatacttaaa caagaagctt ggtccatcta cgaaatgaat gaaaaactag
    10441 aagactcaag acgtaacagt accccatgtt ttgagttcga ggtgcattca cttcctgaat
    10501 acgtattacc gatacaaaat acgcctccca aacgcactca atcagaacca gtacaaccta
    10561 aagctgatga ttatgattac ggtggccctg gtttttaagc tcagttggtt ttatccaaag
    10621 tttacagagc gagtgtctac gagcgaacag acacaattaa aatttttgcc atgctttttc
    10681 cctgattaca aactcccctt tgctgctgag actgagatac agagcatccc ggagggtgcg
    10741 aacagattta atttgcatct aggtcgagcg aagcgagtat aaaaaactta tgagcgaagc
    10801 gaattccgag ttgcttttgc ttttttgaag ttatcacgag attaacccaa aaattgcccc
    10861 ttcgaactga gcgtaagcga agttcaatag agtttgagcg tagcgaaaac atagggcaat
    10921 ttttcattca ttgggctttt aattattttt aatcttttaa atgcttttaa ataagctgta
    10981 aacctttatt tataatagtt tcatagttaa taaaactagg tttatcgacc cataaaacta
    11041 ggtttatcga cccataaaac taggtttatc gacccataaa actaggttta tcgaccttaa
    11101 agctaggttt atcgacccat aattaagtta atttacaact caattgaaat agtctattgt
    11161 acaacctagt ttaattataa atttattatt agaa
//
