LOCUS       NZ_CP077842            11194 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Acinetobacter baumannii strain DETAB-P90 plasmid pDETAB9a, complete
            sequence.
ACCESSION   NZ_CP077842
VERSION     NZ_CP077842.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19774653
            Assembly: GCF_023517695.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Liu,H., Moran,R.A., Doughty,E.L., Hua,X., Ba,X., Jiang,Y.,
            Holmes,M., Mcnally,A., Van Schaik,W. and Yu,Y.
  TITLE     DETECTIVE: Carbapenem-resistant Acinetobacter baumannii study 2019
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11194)
  AUTHORS   Liu,H., Moran,R.A., Doughty,E.L., Hua,X., Ba,X., Jiang,Y.,
            Holmes,M., Mcnally,A., Van Schaik,W. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2021) Department of Infectious Diseases, Zhejiang
            University School of Medicine, Qingchundong 3 RD, Hangzhou 310016,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023517695.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 04:46:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,070
            CDSs (total)                      :: 3,973
            Genes (coding)                    :: 3,890
            CDSs (with protein)               :: 3,890
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 34 of 83
            Pseudo Genes (incomplete)         :: 42 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077842.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="DETAB-P90"
                     /isolation_source="Rectal swab_P131_BU4"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pDETAB9a"
                     /geo_loc_name="China: Hangzhou, Sir Run Run Shaw Hospital
                     ICU"
                     /lat_lon="30.256965 N 120.201625 E"
                     /collection_date="2019-10-29"
                     /collected_by="Yunsong Yu"
     gene            1..936
                     /gene="repM"
                     /locus_tag="KU251_RS20320"
                     /old_locus_tag="KU251_20210"
     CDS             1..936
                     /gene="repM"
                     /locus_tag="KU251_RS20320"
                     /old_locus_tag="KU251_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001292329.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLREASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YESQSGIIFLRFTQDIVPLITRLEENFTKYELQQVSRLSSSYAIRLYELLIQWRSAGKT
                     PLFDLSIFRQQLGVKPHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFTFKQKKMSNLPIKNKRDPDTIDIFSKMTDAQRHLFSHKLSELPEMGKYSHGTESYPQ
                     FAVRIAEMLQNPEKFKELYPYLQKVGFKAA"
                     /name="repM"
     gene            1014..1532
                     /locus_tag="KU251_RS20325"
                     /old_locus_tag="KU251_20215"
     CDS             1014..1532
                     /locus_tag="KU251_RS20325"
                     /old_locus_tag="KU251_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000839337.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPVQKQSVKETVKVDSPTSAEVLLLRQQIDLLKNQLDDAKNRESFYQNQIETMQRLL
                     EAPKTNTTQSDDPGSTQAESTVEEKEDVIAMPPKESEVIENKRIPVPEHIEPEPEKRGF
                     WSRFFKPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            1742..2041
                     /locus_tag="KU251_RS20330"
                     /old_locus_tag="KU251_20220"
     CDS             1742..2041
                     /locus_tag="KU251_RS20330"
                     /old_locus_tag="KU251_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            2607..2963
                     /locus_tag="KU251_RS20335"
                     /old_locus_tag="KU251_20225"
     CDS             2607..2963
                     /locus_tag="KU251_RS20335"
                     /old_locus_tag="KU251_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2956..3258
                     /locus_tag="KU251_RS20340"
                     /old_locus_tag="KU251_20230"
     CDS             2956..3258
                     /locus_tag="KU251_RS20340"
                     /old_locus_tag="KU251_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            3251..3460
                     /locus_tag="KU251_RS20345"
                     /old_locus_tag="KU251_20235"
     CDS             3251..3460
                     /locus_tag="KU251_RS20345"
                     /old_locus_tag="KU251_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069474.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(3786..4247)
                     /locus_tag="KU251_RS20350"
                     /old_locus_tag="KU251_20240"
     CDS             complement(3786..4247)
                     /locus_tag="KU251_RS20350"
                     /old_locus_tag="KU251_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(4552..6963)
                     /gene="znuD2"
                     /locus_tag="KU251_RS20355"
                     /old_locus_tag="KU251_20245"
     CDS             complement(4552..6963)
                     /gene="znuD2"
                     /locus_tag="KU251_RS20355"
                     /old_locus_tag="KU251_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            complement(7091..7405)
                     /locus_tag="KU251_RS20360"
                     /old_locus_tag="KU251_20250"
     CDS             complement(7091..7405)
                     /locus_tag="KU251_RS20360"
                     /old_locus_tag="KU251_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(7392..7679)
                     /locus_tag="KU251_RS20365"
                     /old_locus_tag="KU251_20255"
     CDS             complement(7392..7679)
                     /locus_tag="KU251_RS20365"
                     /old_locus_tag="KU251_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438827.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            complement(7947..8246)
                     /locus_tag="KU251_RS20370"
                     /old_locus_tag="KU251_20260"
     CDS             complement(7947..8246)
                     /locus_tag="KU251_RS20370"
                     /old_locus_tag="KU251_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            complement(8363..9124)
                     /locus_tag="KU251_RS20375"
                     /old_locus_tag="KU251_20265"
     CDS             complement(8363..9124)
                     /locus_tag="KU251_RS20375"
                     /old_locus_tag="KU251_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            9429..10598
                     /locus_tag="KU251_RS20380"
                     /old_locus_tag="KU251_20270"
     CDS             9429..10598
                     /locus_tag="KU251_RS20380"
                     /old_locus_tag="KU251_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002134206.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFEVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     Typing          2..936
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9508..10595
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagtgaat taatcgtaaa ggataatgct ctaattcaag caagttatac cttagataca
       61 gttgaacaaa gactgatcct attagccatt gctgaagctc gagaaactgg acatgggata
      121 actgaaaata gtcttttaga agtacatgca agtagttata taaatacttt taatgtcgag
      181 aaacataccg cttataccgt actcagagag gcatctaaaa gcttatttga tcgctatgtc
      241 acataccatg acattaatcc taagacaggt aaggatcgta gctttcattg tcgttgggtc
      301 gacaaaattg ggtatgaatc tcaatcagga attattttcc tacgattcac ccaagatatc
      361 gttccactca taactcgact tgaagaaaat ttcactaaat atgaattgca gcaggtttct
      421 aggttaagta gctcatatgc tattcggcta tacgagctat taattcaatg gagatctgcc
      481 ggaaaaacgc cactttttga tctatctatc tttagacaac aacttggtgt caaacctcat
      541 caatacaaaa caatgagtaa ctttaaaaca tatgttttag attttgctct taagcaggta
      601 aatgagttaa ccgatataac agctaaatat gagcaacata aaaaagggcg ttctatttca
      661 ggtttttcat tcactttcaa acagaaaaaa atgagtaatc tgccaataaa aaataagcgt
      721 gacccagaca ctatagatat tttctcaaaa atgacagatg ctcaacgcca tctgttttcc
      781 cacaaactgt cagaacttcc tgaaatggga aagtattctc atggtacaga aagctatccg
      841 caattcgctg tacgcattgc agaaatgctt caaaacccag aaaagtttaa agaactctat
      901 ccttatctcc aaaaagtagg cttcaaagct gcatagaaga tagacttgct gatttgtcta
      961 ttagacagtc taatagacac taatttttta caagattagg cagagattta cacatgaaaa
     1021 gccttacagt tcttgagctt tctaagcttt acaatattaa tcgacaaacc atttacaaca
     1081 atataaagaa aggtatttta agtaaaaatt ctgacaataa gattgatctt gcagaagcga
     1141 tccgggtatt tggtgagcca gtacaaaaac agagtgtaaa agaaactgta aaagtagaca
     1201 gtccaacttc ggcagaagtt ttactcctta gacagcagat agacctacta aaaaatcagc
     1261 tagatgatgc aaaaaataga gaaagcttct atcaaaatca aattgaaact atgcaacgcc
     1321 tattggaagc tccaaaaacc aatacaacac aatctgatga tcctggatca acacaggcag
     1381 aatccacagt agaagaaaaa gaggacgtca tagctatgcc tcctaaggag tcagaggtta
     1441 tcgagaataa gcgtattcca gtccctgaac atattgagcc agaaccagaa aaaaggggct
     1501 tctggagccg ttttttcaag ccctatggat gataggacta tttaatcaga ctaatcaaaa
     1561 ccgtggaaca atgatcaata attgatcgtg aaacataaaa aatcactaaa acagcctgag
     1621 cgtgactgct taggctgttt ttacatccat aagacattgt tttaaaatgc tttttacaaa
     1681 gaatttcgta taaggtgtat tatgttaatt ttagggaaac ttaattctat aggtgatacc
     1741 aatggacaaa aagatagaag agaaaactaa agatattaat gaaaaaattc aaaaattgga
     1801 tttagctatt caagaactaa tagaaactac agacaatttt tttattaatg ataagtacta
     1861 tggaattgaa tgtgctttta ttttagacca tgcactaaat attaaaatgc actttttcaa
     1921 caagtatcca gcatttgcac cacctcactt acgaggagta gaaaattatg atcgaattat
     1981 acatattaga gaaaataatc ctgaagataa gctagaacaa atttctcctc ttaaagacta
     2041 aattttctaa tcttgtactt agagtcccct actatttaac ataatggata ttatgcgaaa
     2101 tggatcttta agattacttt aagatcagta gcttacattg agtcataccc tctggaacga
     2161 cctcgttcaa ccgacttaga aacaagtcgg tttttttatg ctcaattttg atacttcttg
     2221 ccaataaaat tgtttaaaaa ataatcaaaa atagcaattc catagagttt ataagctttt
     2281 ttacccaaaa gaatccacta tttctgtgga taaccactgg gataaatttt gattattttt
     2341 tgtacgtttt ggcttacagc gtttcacgaa attgtctgta tctatccccc gatgaagctc
     2401 ttatgatcga tctatatgga caggatgttc aatgacttaa aacaacaata ataatcagtc
     2461 aggcaccatg accatcaggt agtcagccct acctatgcgc tccataccga aaaatcccga
     2521 catgacgtcg ggatttttta ttgccatttg caaagtcatt caatcagatt taatatatag
     2581 actgtaatct atattgtgat tgtaatatgt ggacagtcat tacaacggat ctgtttaatg
     2641 aatggcttga acagcaagat gaagcaacac aagagaaagt cttagctgca ttggttgttc
     2701 tacaacagca agggcctagt ttaggtcgtc ctttagtaga taccgtctac gattctaaat
     2761 ttaccaatat gaaggaacta cgtgttcagc atcgtggtaa accgttaagg gcattttttg
     2821 cattcgatcc cctaagacaa gcgattgtgc tgtgtattgg tgataagagt aataaaaagc
     2881 gtttttatac agagatgcta gcaattgcag atgagcaata cgcacttcat ctctcaactt
     2941 taggagatca atctaatggc taagagctta caagaattat tggctagtcg ctctccagag
     3001 agtcaagctc gtattcaaaa aatggctgat gaattacttt tagaaagtca gcttcacctt
     3061 atccgagaag agttagaaat ttcccagaaa gaactagctg caacccttgg aattaaacag
     3121 ccgtcattat cagcaatcga aaatcgtggt catgatctta aaatttcaac gatgaaaaag
     3181 tatgttgaag caatgggtgg aaaacttcgt attgatgtag agcttccaac aggaaaacac
     3241 atagggttca atgtctaata ataaaccttt gaaaaatagt actaaattac ttgttaactt
     3301 agacaagttt atatttttag tcaatgctgc cgatagccta gaagaaattg aaattattag
     3361 agatttatgc tgtgaatatt tttcacattg taagaggcca agctattaca ttgatatttt
     3421 tgacaatgct tattggataa aatattacga aatagcttaa tccttctaga ttcgcataag
     3481 agattttatg ttaaatgccg aaatgaatta aaaatagaaa tgtagttgag ctgatgcttg
     3541 ctcatttctt tatttttagt aattgaggtg aacatactgt cagtacatgg tagttataag
     3601 taacgtgtaa ctttgtttgc acaaagattt gagttatgcc aagtgatgga ttgatttgta
     3661 cttaaaattt taagcttatc cacttgcttt gcaagccact acactttatt tacaatccta
     3721 tgttgtggaa gtaagggatc ttgtactcgc ccagtatgtt caacttgatt atatctaaag
     3781 gactattagg cttcaatcaa atcaattttc caattagtcg actgaattaa cacgtttaaa
     3841 tcacgtaatt taagactaat atcatcagct tgtttttgta atgatgaaac tggaataact
     3901 ttatgccact ttatttcccg cgtactataa cgatcagatt cacgagatgt attacttatt
     3961 gctgcagtta gggttttgtg ctgttctaca aattcatcac gcaaagtcaa taaagcaagt
     4021 agagaacggc catcatttaa ttttgttaaa gcattagttt catgaattgc ataacttaat
     4081 ttttgaaatt cttgaattaa agaaaatact tgttttagta gttcatttgg atcttcagat
     4141 ggttgatcac catcttgtac caatacattt gcattgatac gttgttttag tgaaataatt
     4201 tttttttgtt gatcacttcg aagtaagaga gcttctgcta atttcatttt tcacctatta
     4261 accaattaga attaagtttt aaacagctta tatcctcatt ctttaaagat caaaaaaatt
     4321 gctttttatc tagctaaaag gtgtctacaa aatcggtggc tattcagtgc tgaaaaggct
     4381 gccgacacat aaagtgaccc agattgaaga gttactgcca cactgctgga aacctaaatc
     4441 gaattaaaaa ataggtatgg gattcagcgg acgcttacct taattattaa tctaatcatt
     4501 ttataagaaa aatcttctaa gaaattttct tagaagatta cagtatctta tctagaaatt
     4561 aagatttaag cctaaactaa aatttcttcc aatttgggga atatatggca aaaatgtttc
     4621 atgggcatag actttctgat caagtaaatt attcgcttta aagaataaat catattctgt
     4681 gtgagatagc ttatttttat atgctaaagt catattaagc atattataac ctgatgtaac
     4741 ctgctcaaat ttagaaatat tatcttgttt aaagactcga taatattcta tttcaccttt
     4801 aagcgattca tcaaaatcag cttttagtcg tgtacccaaa cgagcaggag gtaatctagg
     4861 agtgtaacga tccttttggg gagctaaagt cggttctcta ttccagatat cgtatgcaat
     4921 tacagcatca ggtaaatttg ttaaacggcc tttcacataa tctccaaaaa gagagccgtg
     4981 ataaacagaa ttaaattgat accctatgtt gccctctaat ccataaaaat tagcagcaga
     5041 ctggctataa tgattaatac gtagtgtatg ctgatctcta acttttgtag ttcctaaaac
     5101 ttcattgagt gtctgtaaat atatatagtt atcaaaatca taatagtaag tactgatttg
     5161 ataatcaagt aaatcatcct tataggccag actgatttct aaattattgg atttctcttt
     5221 tcttagaaag cggttaccta tttcaaaaga attagtagcg agatgcatac catgagtata
     5281 catttcttgt gcattaggta atctttcttg atgtgtacct gttaatgata agatataatt
     5341 tggagcaaat ctccagtgga ctgtacctgc ataagaaaaa gctgtttctt tgttaggttg
     5401 aacagcttct aaaatagatt ttaaattttg agccctgatt ttattatttt tttctacata
     5461 aggactgtta tatttattag gccaaggttt cattgaatct ttaatttttt ctatatcata
     5521 atccatacta actttctgct tttctatacg agcacctagt tcgaaagtaa agtcgttcca
     5581 gttatatttt tctaggccaa atacactaaa gtttttagtg acatttcgat ttaatagctg
     5641 ctgattatct agataggtgg tatggccttc ttgagaatgg actggtgata aagcactatt
     5701 atcctgttcc aaatattgta gccccaagat accggttaat tcccctaatg gctgatgtga
     5761 taactctatg cggcctactt ttcctgtatt tttaaaaaag ttagtaatct tatctccctc
     5821 gagttcattg tgaaaataat ctatataact caatgaggta cgaattttgt caataccggt
     5881 aaatggttga gtgaattctc cacgcacatc ataacgtcga gtatttaaat caatataagg
     5941 agcattatga gagaaattag tttcatgaat atgattatgc tgatgacaat caggacgtgg
     6001 atttatgtaa ttaatatctt gctcatccat caattgagga taatataaaa gataattttt
     6061 taatccacta attcgtgaat cgataccgat tgcagaggca ccacaacctt catataaatg
     6121 gctatgggct ggtaaaccat attcatcatg acgatgtgta taagaaactc ctaaataagt
     6181 attatcgtct atccaggata atccaagcgt acctactctc gactctgccc agctttctgg
     6241 taaatatttt aaatttttat aaatactgtt ttggttttca agatcttcta aagaattata
     6301 agagctagac tggtaatgag gagttttata gttaccagca ttacgatata acccttctgc
     6361 ccgtaaagct acatgtgggc ttaagccaac tgttacacct gctgtggtca acttctcatt
     6421 actgccagta ttaaaacgta atgtagtatt accctctaat ccgtcttgag gcatttgggt
     6481 tggaatttta tagtcaatga cattgactac ccctgccgca ctatttgaac tatacaaaag
     6541 ggtacttgca ccacgtataa tttcaataga cttagctaaa cttggttcaa ctgtgacagc
     6601 gtgatctgga gacatattag acatatccaa cacgtcagca ttattctgta aaactttaat
     6661 acgttttcct tcctgcccac gaataatagg agtagatgct ccacctccat attgattaga
     6721 atgaattcct aattccgtac ccaaggcatc ccctagagtt gtcgaacgct gtttaagttt
     6781 actagtatca ataaactgat tactaacatt tagacttttg cctaacagct cagaccctaa
     6841 ctcatcagat tgtgcagtta atactattgt atttaaggta gtaacaggcc cttcagctgc
     6901 caataaaatt ttcgaggccc ctaaagtaat taaagctaaa aatagtttac ttttatttag
     6961 cataaaacaa ccaaatttaa tgttatatta taatataaca cttaaaaatt tataaaatct
     7021 actatttaaa tttttgaatg gtaatccccc ctaaaaatga agtcaaaaat gggagcctaa
     7081 gctcccattc ttattgatga ttttttaatt cttttagcat agcatctcgt aaaatttcat
     7141 tcatacgtgt ttgataacct ttgccttgtg ctttgaacca agcaagtaca tcagcatcta
     7201 accgaattga agtttgttgc tttactgggc ggtaaaattg attttggcgt acagcattgc
     7261 tccagtctgt aatttcagga atatctgata gatctagctg atcatcagga accgtgcctt
     7321 tagcaagcaa gcgttgaatt tcagcatctt gcttctcacc aaatttctca ttcagctctt
     7381 tgtgtgagta tctaaccatg ctcatatttg tttcgctccg ctttagtgac ttgccttgca
     7441 ctaatgattc ggatgatttc acagtcatct tcatcaaaaa tggtgtgagc caccaataac
     7501 attagtacgc ctttaactcg tccaatggtt tgccaacgtt cttcaccatt agtatatctg
     7561 tcttggattg agatccgtaa tggatcttca aaaacaaggc ttgcagtttc gaaagagata
     7621 tcatgctttt tctgattctt tcgattctta gcctcatccc attcaaaata ctgttccata
     7681 aaaaatattc aaatttgtat atacaataat gtatcacgaa attgtatcac tcaagttgag
     7741 aagcgtaatt tttagtcaga tttattggca aaaactatca aaatcgatca taaaaaagcc
     7801 gacttgtttc aaagtcggct ttttgggcat ttttggattt ttatattttt attaagtagt
     7861 tgatatttaa attaatatac aagtgtactt cgtataatat ccattatgtt aaatagtagg
     7921 ggactctaag tacaagatta gaaaatttag tctttaagag gagaaatttg ttctagctta
     7981 tcttcaggat tattttctct aatatgtata attcgatcat aattttctac tcctcgtaag
     8041 tgaggtggtg caaatgctgg atacttgttg aaaaagtgca ttttaatatt tagtgcatgg
     8101 tctaaaataa aagcacattc aattccatag tacttatcat taataaaaaa attgtctgta
     8161 gtttctatta gttcttgaat agctaaatcc aatttttgaa ttttttcatt aatatcttta
     8221 gttttctctt ctatcttttt gtccattggt atcacctata gaattaagtt tccctaaaat
     8281 taacataata caccttatgc gaaattccaa tgtaagccca aaatagaaat gtccaacttc
     8341 tctaaaatcc ctgctaaata aactactctt cgcctttttc taaggccttt ttaatttcaa
     8401 ttactgacaa taattctccg cccttttgcc aggtatgtaa tccttctaac acctgaataa
     8461 aaagtttatt tgctttaaaa tattcatttt catatgtctt aacatcttta aaaattaaag
     8521 atttacgtac ttcattatga aactttgtcg tattcacaat ccgattggta atctgttgag
     8581 gagtatcatt gctaatatca atattgagtt tcttaaatgc gacaagaaca gcaccaccta
     8641 attcaaattt acgttgattt tcagctttgc gttttttccc agcatccttc ttctgtagct
     8701 gactcaaata caattctaaa tcactgtatt caagcttttg cgctttaaaa ttcagatacc
     8761 gtgtcgccaa ggtttttaaa atatttagtt ccatctgatc tagtgcattc ttttgaaaaa
     8821 gttcatacag atctaaaaac ttctgttcat actcggtttt aagaggcaga ttattcaact
     8881 cttcaaacag caatttcaac ttcagatgtt cacgataagg ttttaaagcc tctttgacca
     8941 gaacctgatc tgtctgagtt agcccctcaa aattacaatc aacactattt aaagccttta
     9001 acacttgaga ttgaagatca gaaagctgtt cttgctgttc taaaaactct acaaatgcat
     9061 gtaactcagc aatctgtaac aactgagcag gtcgaccacg cttttttgct ttttccattt
     9121 ccatcatctg ccgccaaatt ttcagaactg caacctcttt aaatatggtt ttaccatatt
     9181 taaagagggc agtctgaaaa taattattta atataaaaat aaaaattctt ttcaagaaat
     9241 tttattttta tattaaataa ttcgcacttg acagtttctc ctcatcggag aaactagtac
     9301 gcaatggggc gcttccccct tgaccccctt caaccggatt tttcagccat acaggcattt
     9361 caaatccggg cggttttcat tccgtttcac tgcataaaaa ccgaacccac aacccagcag
     9421 aggtgaacat ggccagctac cacttcagcg tcaaaagcaa aaataagggc tatgcgaccg
     9481 gccattatct ctacatttcc cggctgatgc agtatgaagg catccgcaaa accagcaatg
     9541 agaccataga acatattgaa cctggtcgat gtatgcctgc ctttgtaaaa gatcccattg
     9601 atttttggca ggcagcagat acgtatgaac gagccaatgc caaggcctac atggagtatg
     9661 aaattgccct accgaatgag ttcacgtcag aacagcgtaa aacgctcata gagacgtttt
     9721 tagacaaaca tatagttcca cagcaatatc cgcactcata cgccatacac aacgtaaaat
     9781 cacgtatcag tggcgaagat cagcctcatt gtcatctgat gttctcgcta aaagcggatg
     9841 acggcattga acgcagcgca gaacagtact tcaaacgcta taaccctcaa gatccagcca
     9901 aaggcggtgc caaaaaaatc caacttcaag atggccatga agactattcc acttttttga
     9961 tctatatccg aaaacaatgg gaaaatcatt tgaatgatgc ccttgcacag cactgcccga
    10021 ccgtcaccta cacactggat ggccaagaca tcaccatcaa aaatcaggtt tcagcagaca
    10081 gctatgaaaa atataacgag atccacagca cgctatacct tccagagcca aaactaggcg
    10141 ttggacaaca aaatgcgaca gcggaatatc tcgcccaaat ccaaaaaatc agagaacaca
    10201 accagcaaga acgagaactc gaagaacgcc aaaagcaatt agatcaacaa catgaatatt
    10261 tttatagtgc tgaaaacaac ccattttcat cattggaagt catgtattac ctgaattact
    10321 taatcaagaa tgcaacaaat aaggacaaac taaaaaatga tttgaatgaa attatcaaaa
    10381 accaaggccc tatacttaaa caagaagctt ggtccatcta cgaaatgaat gaaaaactag
    10441 aagactcaag acgtaacagt accccatgtt ttgagttcga ggtgcattca cttcctgaat
    10501 acgtattacc gatacaaaat acgcctccca aacgcactca atcagaacca gtacaaccta
    10561 aagctgatga ttatgattac ggtggccctg gtttttaagc tcagttggtt ttatccaaag
    10621 tttacagagc gagtgtctac gagcgaacag acacaattaa aatttttgcc atgctttttc
    10681 cctgattaca aactcccctt tgctgctgag actgagatac agagcatccc ggagggtgcg
    10741 aacagattta atttgcatct aggtcgagcg aagcgagtat aaaaaactta tgagcgaagc
    10801 gaattccgag ttgcttttgc ttttttgaag ttatcacgag attaacccaa aaattgcccc
    10861 ttcgaactga gcgtaagcga agttcaatag agtttgagcg tagcgaaaac atagggcaat
    10921 ttttcattca ttgggctttt aattattttt aatcttttaa atgcttttaa ataagctgta
    10981 aacctttatt tataatagtt tcatagttaa taaaactagg tttatcgacc cataaaacta
    11041 ggtttatcga cccataaaac taggtttatc gacccataaa actaggttta tcgaccttaa
    11101 agctaggttt atcgacccat aattaagtta atttacaact caattgaaat agtctattgt
    11161 acaacctagt ttaattataa atttattatt agaa
//
