LOCUS       NZ_CP077831            11193 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Acinetobacter baumannii strain DETAB-E51 plasmid pDETAB9b, complete
            sequence.
ACCESSION   NZ_CP077831
VERSION     NZ_CP077831.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19762528
            Assembly: GCF_023517195.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11193)
  AUTHORS   Liu,H., Moran,R.A., Doughty,E.L., Hua,X., Ba,X., Jiang,Y.,
            Holmes,M., Mcnally,A., Van Schaik,W. and Yu,Y.
  TITLE     DETECTIVE: Carbapenem-resistant Acinetobacter baumannii study 2019
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11193)
  AUTHORS   Liu,H., Moran,R.A., Doughty,E.L., Hua,X., Ba,X., Jiang,Y.,
            Holmes,M., Mcnally,A., Van Schaik,W. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2021) Department of Infectious Diseases, Zhejiang
            University School of Medicine, Qingchundong 3 RD, Hangzhou 310016,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023517195.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 04:24:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,156
            CDSs (total)                      :: 4,059
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 22 of 67
            Pseudo Genes (incomplete)         :: 39 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077831.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11193
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="DETAB-E51"
                     /isolation_source="Bed controller_P22_BU23"
                     /db_xref="taxon:470"
                     /plasmid="pDETAB9b"
                     /geo_loc_name="China: Hangzhou, Sir Run Run Shaw Hospital
                     ICU"
                     /lat_lon="30.256965 N 120.201625 E"
                     /collection_date="2019-08-13"
                     /collected_by="Yunsong Yu"
     gene            1..936
                     /gene="repM"
                     /locus_tag="KU058_RS20755"
                     /old_locus_tag="KU058_20650"
     CDS             1..936
                     /gene="repM"
                     /locus_tag="KU058_RS20755"
                     /old_locus_tag="KU058_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001292329.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLREASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YESQSGIIFLRFTQDIVPLITRLEENFTKYELQQVSRLSSSYAIRLYELLIQWRSAGKT
                     PLFDLSIFRQQLGVKPHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFTFKQKKMSNLPIKNKRDPDTIDIFSKMTDAQRHLFSHKLSELPEMGKYSHGTESYPQ
                     FAVRIAEMLQNPEKFKELYPYLQKVGFKAA"
                     /name="repM"
     gene            1014..1532
                     /locus_tag="KU058_RS20760"
                     /old_locus_tag="KU058_20655"
     CDS             1014..1532
                     /locus_tag="KU058_RS20760"
                     /old_locus_tag="KU058_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000839337.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPVQKQSVKETVKVDSPTSAEVLLLRQQIDLLKNQLDDAKNRESFYQNQIETMQRLL
                     EAPKTNTTQSDDPGSTQAESTVEEKEDVIAMPPKESEVIENKRIPVPEHIEPEPEKRGF
                     WSRFFKPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            1742..2041
                     /locus_tag="KU058_RS20765"
                     /old_locus_tag="KU058_20660"
     CDS             1742..2041
                     /locus_tag="KU058_RS20765"
                     /old_locus_tag="KU058_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            2606..2962
                     /locus_tag="KU058_RS20770"
                     /old_locus_tag="KU058_20665"
     CDS             2606..2962
                     /locus_tag="KU058_RS20770"
                     /old_locus_tag="KU058_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2955..3257
                     /locus_tag="KU058_RS20775"
                     /old_locus_tag="KU058_20670"
     CDS             2955..3257
                     /locus_tag="KU058_RS20775"
                     /old_locus_tag="KU058_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            3250..3459
                     /locus_tag="KU058_RS20780"
                     /old_locus_tag="KU058_20675"
     CDS             3250..3459
                     /locus_tag="KU058_RS20780"
                     /old_locus_tag="KU058_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069474.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(3785..4246)
                     /locus_tag="KU058_RS20785"
                     /old_locus_tag="KU058_20680"
     CDS             complement(3785..4246)
                     /locus_tag="KU058_RS20785"
                     /old_locus_tag="KU058_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(4551..6962)
                     /gene="znuD2"
                     /locus_tag="KU058_RS20790"
                     /old_locus_tag="KU058_20685"
     CDS             complement(4551..6962)
                     /gene="znuD2"
                     /locus_tag="KU058_RS20790"
                     /old_locus_tag="KU058_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            complement(7090..7404)
                     /locus_tag="KU058_RS20795"
                     /old_locus_tag="KU058_20690"
     CDS             complement(7090..7404)
                     /locus_tag="KU058_RS20795"
                     /old_locus_tag="KU058_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(7391..7678)
                     /locus_tag="KU058_RS20800"
                     /old_locus_tag="KU058_20695"
     CDS             complement(7391..7678)
                     /locus_tag="KU058_RS20800"
                     /old_locus_tag="KU058_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438827.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            complement(7946..8245)
                     /locus_tag="KU058_RS20805"
                     /old_locus_tag="KU058_20700"
     CDS             complement(7946..8245)
                     /locus_tag="KU058_RS20805"
                     /old_locus_tag="KU058_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            complement(8362..9123)
                     /locus_tag="KU058_RS20810"
                     /old_locus_tag="KU058_20705"
     CDS             complement(8362..9123)
                     /locus_tag="KU058_RS20810"
                     /old_locus_tag="KU058_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            9428..10597
                     /locus_tag="KU058_RS20815"
                     /old_locus_tag="KU058_20710"
     CDS             9428..10597
                     /locus_tag="KU058_RS20815"
                     /old_locus_tag="KU058_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002134206.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFEVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     Typing          2..936
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9507..10594
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagtgaat taatcgtaaa ggataatgct ctaattcaag caagttatac cttagataca
       61 gttgaacaaa gactgatcct attagccatt gctgaagctc gagaaactgg acatgggata
      121 actgaaaata gtcttttaga agtacatgca agtagttata taaatacttt taatgtcgag
      181 aaacataccg cttataccgt actcagagag gcatctaaaa gcttatttga tcgctatgtc
      241 acataccatg acattaatcc taagacaggt aaggatcgta gctttcattg tcgttgggtc
      301 gacaaaattg ggtatgaatc tcaatcagga attattttcc tacgattcac ccaagatatc
      361 gttccactca taactcgact tgaagaaaat ttcactaaat atgaattgca gcaggtttct
      421 aggttaagta gctcatatgc tattcggcta tacgagctat taattcaatg gagatctgcc
      481 ggaaaaacgc cactttttga tctatctatc tttagacaac aacttggtgt caaacctcat
      541 caatacaaaa caatgagtaa ctttaaaaca tatgttttag attttgctct taagcaggta
      601 aatgagttaa ccgatataac agctaaatat gagcaacata aaaaagggcg ttctatttca
      661 ggtttttcat tcactttcaa acagaaaaaa atgagtaatc tgccaataaa aaataagcgt
      721 gacccagaca ctatagatat tttctcaaaa atgacagatg ctcaacgcca tctgttttcc
      781 cacaaactgt cagaacttcc tgaaatggga aagtattctc atggtacaga aagctatccg
      841 caattcgctg tacgcattgc agaaatgctt caaaacccag aaaagtttaa agaactctat
      901 ccttatctcc aaaaagtagg cttcaaagct gcatagaaga tagacttgct gatttgtcta
      961 ttagacagtc taatagacac taatttttta caagattagg cagagattta cacatgaaaa
     1021 gccttacagt tcttgagctt tctaagcttt acaatattaa tcgacaaacc atttacaaca
     1081 atataaagaa aggtatttta agtaaaaatt ctgacaataa gattgatctt gcagaagcga
     1141 tccgggtatt tggtgagcca gtacaaaaac agagtgtaaa agaaactgta aaagtagaca
     1201 gtccaacttc ggcagaagtt ttactcctta gacagcagat agacctacta aaaaatcagc
     1261 tagatgatgc aaaaaataga gaaagcttct atcaaaatca aattgaaact atgcaacgcc
     1321 tattggaagc tccaaaaacc aatacaacac aatctgatga tcctggatca acacaggcag
     1381 aatccacagt agaagaaaaa gaggacgtca tagctatgcc tcctaaggag tcagaggtta
     1441 tcgagaataa gcgtattcca gtccctgaac atattgagcc agaaccagaa aaaaggggct
     1501 tctggagccg ttttttcaag ccctatggat gataggacta tttaatcaga ctaatcaaaa
     1561 ccgtggaaca atgatcaata attgatcgtg aaacataaaa aatcactaaa acagcctgag
     1621 cgtgactgct taggctgttt ttacatccat aagacattgt tttaaaatgc tttttacaaa
     1681 gaatttcgta taaggtgtat tatgttaatt ttagggaaac ttaattctat aggtgatacc
     1741 aatggacaaa aagatagaag agaaaactaa agatattaat gaaaaaattc aaaaattgga
     1801 tttagctatt caagaactaa tagaaactac agacaatttt tttattaatg ataagtacta
     1861 tggaattgaa tgtgctttta ttttagacca tgcactaaat attaaaatgc actttttcaa
     1921 caagtatcca gcatttgcac cacctcactt acgaggagta gaaaattatg atcgaattat
     1981 acatattaga gaaaataatc ctgaagataa gctagaacaa atttctcctc ttaaagacta
     2041 aattttctaa tcttgtactt agagtcccct actatttaac ataatggata tatgcgaaat
     2101 ggatctttaa gattacttta agatcagtag cttacattga gtcataccct ctggaacgac
     2161 ctcgttcaac cgacttagaa acaagtcggt ttttttatgc tcaattttga tacttcttgc
     2221 caataaaatt gtttaaaaaa taatcaaaaa tagcaattcc atagagttta taagcttttt
     2281 tacccaaaag aatccactat ttctgtggat aaccactggg ataaattttg attatttttt
     2341 gtacgttttg gcttacagcg tttcacgaaa ttgtctgtat ctatcccccg atgaagctct
     2401 tatgatcgat ctatatggac aggatgttca atgacttaaa acaacaataa taatcagtca
     2461 ggcaccatga ccatcaggta gtcagcccta cctatgcgct ccataccgaa aaatcccgac
     2521 atgacgtcgg gattttttat tgccatttgc aaagtcattc aatcagattt aatatataga
     2581 ctgtaatcta tattgtgatt gtaatatgtg gacagtcatt acaacggatc tgtttaatga
     2641 atggcttgaa cagcaagatg aagcaacaca agagaaagtc ttagctgcat tggttgttct
     2701 acaacagcaa gggcctagtt taggtcgtcc tttagtagat accgtctacg attctaaatt
     2761 taccaatatg aaggaactac gtgttcagca tcgtggtaaa ccgttaaggg cattttttgc
     2821 attcgatccc ctaagacaag cgattgtgct gtgtattggt gataagagta ataaaaagcg
     2881 tttttataca gagatgctag caattgcaga tgagcaatac gcacttcatc tctcaacttt
     2941 aggagatcaa tctaatggct aagagcttac aagaattatt ggctagtcgc tctccagaga
     3001 gtcaagctcg tattcaaaaa atggctgatg aattactttt agaaagtcag cttcacctta
     3061 tccgagaaga gttagaaatt tcccagaaag aactagctgc aacccttgga attaaacagc
     3121 cgtcattatc agcaatcgaa aatcgtggtc atgatcttaa aatttcaacg atgaaaaagt
     3181 atgttgaagc aatgggtgga aaacttcgta ttgatgtaga gcttccaaca ggaaaacaca
     3241 tagggttcaa tgtctaataa taaacctttg aaaaatagta ctaaattact tgttaactta
     3301 gacaagttta tatttttagt caatgctgcc gatagcctag aagaaattga aattattaga
     3361 gatttatgct gtgaatattt ttcacattgt aagaggccaa gctattacat tgatattttt
     3421 gacaatgctt attggataaa atattacgaa atagcttaat ccttctagat tcgcataaga
     3481 gattttatgt taaatgccga aatgaattaa aaatagaaat gtagttgagc tgatgcttgc
     3541 tcatttcttt atttttagta attgaggtga acatactgtc agtacatggt agttataagt
     3601 aacgtgtaac tttgtttgca caaagatttg agttatgcca agtgatggat tgatttgtac
     3661 ttaaaatttt aagcttatcc acttgctttg caagccacta cactttattt acaatcctat
     3721 gttgtggaag taagggatct tgtactcgcc cagtatgttc aacttgatta tatctaaagg
     3781 actattaggc ttcaatcaaa tcaattttcc aattagtcga ctgaattaac acgtttaaat
     3841 cacgtaattt aagactaata tcatcagctt gtttttgtaa tgatgaaact ggaataactt
     3901 tatgccactt tatttcccgc gtactataac gatcagattc acgagatgta ttacttattg
     3961 ctgcagttag ggttttgtgc tgttctacaa attcatcacg caaagtcaat aaagcaagta
     4021 gagaacggcc atcatttaat tttgttaaag cattagtttc atgaattgca taacttaatt
     4081 tttgaaattc ttgaattaaa gaaaatactt gttttagtag ttcatttgga tcttcagatg
     4141 gttgatcacc atcttgtacc aatacatttg cattgatacg ttgttttagt gaaataattt
     4201 ttttttgttg atcacttcga agtaagagag cttctgctaa tttcattttt cacctattaa
     4261 ccaattagaa ttaagtttta aacagcttat atcctcattc tttaaagatc aaaaaaattg
     4321 ctttttatct agctaaaagg tgtctacaaa atcggtggct attcagtgct gaaaaggctg
     4381 ccgacacata aagtgaccca gattgaagag ttactgccac actgctggaa acctaaatcg
     4441 aattaaaaaa taggtatggg attcagcgga cgcttacctt aattattaat ctaatcattt
     4501 tataagaaaa atcttctaag aaattttctt agaagattac agtatcttat ctagaaatta
     4561 agatttaagc ctaaactaaa atttcttcca atttggggaa tatatggcaa aaatgtttca
     4621 tgggcataga ctttctgatc aagtaaatta ttcgctttaa agaataaatc atattctgtg
     4681 tgagatagct tatttttata tgctaaagtc atattaagca tattataacc tgatgtaacc
     4741 tgctcaaatt tagaaatatt atcttgttta aagactcgat aatattctat ttcaccttta
     4801 agcgattcat caaaatcagc ttttagtcgt gtacccaaac gagcaggagg taatctagga
     4861 gtgtaacgat ccttttgggg agctaaagtc ggttctctat tccagatatc gtatgcaatt
     4921 acagcatcag gtaaatttgt taaacggcct ttcacataat ctccaaaaag agagccgtga
     4981 taaacagaat taaattgata ccctatgttg ccctctaatc cataaaaatt agcagcagac
     5041 tggctataat gattaatacg tagtgtatgc tgatctctaa cttttgtagt tcctaaaact
     5101 tcattgagtg tctgtaaata tatatagtta tcaaaatcat aatagtaagt actgatttga
     5161 taatcaagta aatcatcctt ataggccaga ctgatttcta aattattgga tttctctttt
     5221 cttagaaagc ggttacctat ttcaaaagaa ttagtagcga gatgcatacc atgagtatac
     5281 atttcttgtg cattaggtaa tctttcttga tgtgtacctg ttaatgataa gatataattt
     5341 ggagcaaatc tccagtggac tgtacctgca taagaaaaag ctgtttcttt gttaggttga
     5401 acagcttcta aaatagattt taaattttga gccctgattt tattattttt ttctacataa
     5461 ggactgttat atttattagg ccaaggtttc attgaatctt taattttttc tatatcataa
     5521 tccatactaa ctttctgctt ttctatacga gcacctagtt cgaaagtaaa gtcgttccag
     5581 ttatattttt ctaggccaaa tacactaaag tttttagtga catttcgatt taatagctgc
     5641 tgattatcta gataggtggt atggccttct tgagaatgga ctggtgataa agcactatta
     5701 tcctgttcca aatattgtag ccccaagata ccggttaatt cccctaatgg ctgatgtgat
     5761 aactctatgc ggcctacttt tcctgtattt ttaaaaaagt tagtaatctt atctccctcg
     5821 agttcattgt gaaaataatc tatataactc aatgaggtac gaattttgtc aataccggta
     5881 aatggttgag tgaattctcc acgcacatca taacgtcgag tatttaaatc aatataagga
     5941 gcattatgag agaaattagt ttcatgaata tgattatgct gatgacaatc aggacgtgga
     6001 tttatgtaat taatatcttg ctcatccatc aattgaggat aatataaaag ataatttttt
     6061 aatccactaa ttcgtgaatc gataccgatt gcagaggcac cacaaccttc atataaatgg
     6121 ctatgggctg gtaaaccata ttcatcatga cgatgtgtat aagaaactcc taaataagta
     6181 ttatcgtcta tccaggataa tccaagcgta cctactctcg actctgccca gctttctggt
     6241 aaatatttta aatttttata aatactgttt tggttttcaa gatcttctaa agaattataa
     6301 gagctagact ggtaatgagg agttttatag ttaccagcat tacgatataa cccttctgcc
     6361 cgtaaagcta catgtgggct taagccaact gttacacctg ctgtggtcaa cttctcatta
     6421 ctgccagtat taaaacgtaa tgtagtatta ccctctaatc cgtcttgagg catttgggtt
     6481 ggaattttat agtcaatgac attgactacc cctgccgcac tatttgaact atacaaaagg
     6541 gtacttgcac cacgtataat ttcaatagac ttagctaaac ttggttcaac tgtgacagcg
     6601 tgatctggag acatattaga catatccaac acgtcagcat tattctgtaa aactttaata
     6661 cgttttcctt cctgcccacg aataatagga gtagatgctc cacctccata ttgattagaa
     6721 tgaattccta attccgtacc caaggcatcc cctagagttg tcgaacgctg tttaagttta
     6781 ctagtatcaa taaactgatt actaacattt agacttttgc ctaacagctc agaccctaac
     6841 tcatcagatt gtgcagttaa tactattgta tttaaggtag taacaggccc ttcagctgcc
     6901 aataaaattt tcgaggcccc taaagtaatt aaagctaaaa atagtttact tttatttagc
     6961 ataaaacaac caaatttaat gttatattat aatataacac ttaaaaattt ataaaatcta
     7021 ctatttaaat ttttgaatgg taatcccccc taaaaatgaa gtcaaaaatg ggagcctaag
     7081 ctcccattct tattgatgat tttttaattc ttttagcata gcatctcgta aaatttcatt
     7141 catacgtgtt tgataacctt tgccttgtgc tttgaaccaa gcaagtacat cagcatctaa
     7201 ccgaattgaa gtttgttgct ttactgggcg gtaaaattga ttttggcgta cagcattgct
     7261 ccagtctgta atttcaggaa tatctgatag atctagctga tcatcaggaa ccgtgccttt
     7321 agcaagcaag cgttgaattt cagcatcttg cttctcacca aatttctcat tcagctcttt
     7381 gtgtgagtat ctaaccatgc tcatatttgt ttcgctccgc tttagtgact tgccttgcac
     7441 taatgattcg gatgatttca cagtcatctt catcaaaaat ggtgtgagcc accaataaca
     7501 ttagtacgcc tttaactcgt ccaatggttt gccaacgttc ttcaccatta gtatatctgt
     7561 cttggattga gatccgtaat ggatcttcaa aaacaaggct tgcagtttcg aaagagatat
     7621 catgcttttt ctgattcttt cgattcttag cctcatccca ttcaaaatac tgttccataa
     7681 aaaatattca aatttgtata tacaataatg tatcacgaaa ttgtatcact caagttgaga
     7741 agcgtaattt ttagtcagat ttattggcaa aaactatcaa aatcgatcat aaaaaagccg
     7801 acttgtttca aagtcggctt tttgggcatt tttggatttt tatattttta ttaagtagtt
     7861 gatatttaaa ttaatataca agtgtacttc gtataatatc cattatgtta aatagtaggg
     7921 gactctaagt acaagattag aaaatttagt ctttaagagg agaaatttgt tctagcttat
     7981 cttcaggatt attttctcta atatgtataa ttcgatcata attttctact cctcgtaagt
     8041 gaggtggtgc aaatgctgga tacttgttga aaaagtgcat tttaatattt agtgcatggt
     8101 ctaaaataaa agcacattca attccatagt acttatcatt aataaaaaaa ttgtctgtag
     8161 tttctattag ttcttgaata gctaaatcca atttttgaat tttttcatta atatctttag
     8221 ttttctcttc tatctttttg tccattggta tcacctatag aattaagttt ccctaaaatt
     8281 aacataatac accttatgcg aaattccaat gtaagcccaa aatagaaatg tccaacttct
     8341 ctaaaatccc tgctaaataa actactcttc gcctttttct aaggcctttt taatttcaat
     8401 tactgacaat aattctccgc ccttttgcca ggtatgtaat ccttctaaca cctgaataaa
     8461 aagtttattt gctttaaaat attcattttc atatgtctta acatctttaa aaattaaaga
     8521 tttacgtact tcattatgaa actttgtcgt attcacaatc cgattggtaa tctgttgagg
     8581 agtatcattg ctaatatcaa tattgagttt cttaaatgcg acaagaacag caccacctaa
     8641 ttcaaattta cgttgatttt cagctttgcg ttttttccca gcatccttct tctgtagctg
     8701 actcaaatac aattctaaat cactgtattc aagcttttgc gctttaaaat tcagataccg
     8761 tgtcgccaag gtttttaaaa tatttagttc catctgatct agtgcattct tttgaaaaag
     8821 ttcatacaga tctaaaaact tctgttcata ctcggtttta agaggcagat tattcaactc
     8881 ttcaaacagc aatttcaact tcagatgttc acgataaggt tttaaagcct ctttgaccag
     8941 aacctgatct gtctgagtta gcccctcaaa attacaatca acactattta aagcctttaa
     9001 cacttgagat tgaagatcag aaagctgttc ttgctgttct aaaaactcta caaatgcatg
     9061 taactcagca atctgtaaca actgagcagg tcgaccacgc ttttttgctt tttccatttc
     9121 catcatctgc cgccaaattt tcagaactgc aacctcttta aatatggttt taccatattt
     9181 aaagagggca gtctgaaaat aattatttaa tataaaaata aaaattcttt tcaagaaatt
     9241 ttatttttat attaaataat tcgcacttga cagtttctcc tcatcggaga aactagtacg
     9301 caatggggcg cttccccctt gacccccttc aaccggattt ttcagccata caggcatttc
     9361 aaatccgggc ggttttcatt ccgtttcact gcataaaaac cgaacccaca acccagcaga
     9421 ggtgaacatg gccagctacc acttcagcgt caaaagcaaa aataagggct atgcgaccgg
     9481 ccattatctc tacatttccc ggctgatgca gtatgaaggc atccgcaaaa ccagcaatga
     9541 gaccatagaa catattgaac ctggtcgatg tatgcctgcc tttgtaaaag atcccattga
     9601 tttttggcag gcagcagata cgtatgaacg agccaatgcc aaggcctaca tggagtatga
     9661 aattgcccta ccgaatgagt tcacgtcaga acagcgtaaa acgctcatag agacgttttt
     9721 agacaaacat atagttccac agcaatatcc gcactcatac gccatacaca acgtaaaatc
     9781 acgtatcagt ggcgaagatc agcctcattg tcatctgatg ttctcgctaa aagcggatga
     9841 cggcattgaa cgcagcgcag aacagtactt caaacgctat aaccctcaag atccagccaa
     9901 aggcggtgcc aaaaaaatcc aacttcaaga tggccatgaa gactattcca cttttttgat
     9961 ctatatccga aaacaatggg aaaatcattt gaatgatgcc cttgcacagc actgcccgac
    10021 cgtcacctac acactggatg gccaagacat caccatcaaa aatcaggttt cagcagacag
    10081 ctatgaaaaa tataacgaga tccacagcac gctatacctt ccagagccaa aactaggcgt
    10141 tggacaacaa aatgcgacag cggaatatct cgcccaaatc caaaaaatca gagaacacaa
    10201 ccagcaagaa cgagaactcg aagaacgcca aaagcaatta gatcaacaac atgaatattt
    10261 ttatagtgct gaaaacaacc cattttcatc attggaagtc atgtattacc tgaattactt
    10321 aatcaagaat gcaacaaata aggacaaact aaaaaatgat ttgaatgaaa ttatcaaaaa
    10381 ccaaggccct atacttaaac aagaagcttg gtccatctac gaaatgaatg aaaaactaga
    10441 agactcaaga cgtaacagta ccccatgttt tgagttcgag gtgcattcac ttcctgaata
    10501 cgtattaccg atacaaaata cgcctcccaa acgcactcaa tcagaaccag tacaacctaa
    10561 agctgatgat tatgattacg gtggccctgg tttttaagct cagttggttt tatccaaagt
    10621 ttacagagcg agtgtctacg agcgaacaga cacaattaaa atttttgcca tgctttttcc
    10681 ctgattacaa actccccttt gctgctgaga ctgagataca gagcatcccg gagggtgcga
    10741 acagatttaa tttgcatcta ggtcgagcga agcgagtata aaaaacttat gagcgaagcg
    10801 aattccgagt tgcttttgct tttttgaagt tatcacgaga ttaacccaaa aattgcccct
    10861 tcgaactgag cgtaagcgaa gttcaataga gtttgagcgt agcgaaaaca tagggcaatt
    10921 tttcattcat tgggctttta attattttta atcttttaaa tgcttttaaa taagctgtaa
    10981 acctttattt ataatagttt catagttaat aaaactaggt ttatcgaccc ataaaactag
    11041 gtttatcgac ccataaaact aggtttatcg acccataaaa ctaggtttat cgaccttaaa
    11101 gctaggttta tcgacccata attaagttaa tttacaactc aattgaaata gtctattgta
    11161 caacctagtt taattataaa tttattatta gaa
//
