LOCUS       NZ_CP077780             9294 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain CDI231 plasmid pCDI231-9.29, complete
            sequence.
ACCESSION   NZ_CP077780
VERSION     NZ_CP077780.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19925006
            Assembly: GCF_019107685.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Chen,L.
  TITLE     Whole-genome sequencing of two K. pneumoniae isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Chen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 111 Ideation Way, Nutley, NJ 07110,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:48:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,878
            CDSs (total)                      :: 5,755
            Genes (coding)                    :: 5,606
            CDSs (with protein)               :: 5,606
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 72 of 149
            Pseudo Genes (incomplete)         :: 83 of 149
            Pseudo Genes (internal stop)      :: 32 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077780.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CDI231"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCDI231-9.29"
     gene            complement(join(8633..9294,1..70))
                     /locus_tag="KUM55_RS25920"
                     /old_locus_tag="KUM55_25925"
     CDS             complement(join(8633..9294,1..70))
                     /locus_tag="KUM55_RS25920"
                     /old_locus_tag="KUM55_25925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(70..2034)
                     /locus_tag="KUM55_RS25925"
                     /old_locus_tag="KUM55_25930"
     CDS             complement(70..2034)
                     /locus_tag="KUM55_RS25925"
                     /old_locus_tag="KUM55_25930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3515..3664)
                     /locus_tag="KUM55_RS25930"
                     /old_locus_tag="KUM55_25935"
     CDS             complement(3515..3664)
                     /locus_tag="KUM55_RS25930"
                     /old_locus_tag="KUM55_25935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3749..4006)
                     /locus_tag="KUM55_RS25935"
                     /old_locus_tag="KUM55_25940"
     CDS             complement(3749..4006)
                     /locus_tag="KUM55_RS25935"
                     /old_locus_tag="KUM55_25940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(4016..5701)
                     /locus_tag="KUM55_RS25940"
                     /old_locus_tag="KUM55_25945"
     CDS             complement(4016..5701)
                     /locus_tag="KUM55_RS25940"
                     /old_locus_tag="KUM55_25945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6104..6523
                     /locus_tag="KUM55_RS25945"
                     /old_locus_tag="KUM55_25950"
     CDS             6104..6523
                     /locus_tag="KUM55_RS25945"
                     /old_locus_tag="KUM55_25950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            6829..7092
                     /locus_tag="KUM55_RS25950"
                     /old_locus_tag="KUM55_25955"
     CDS             6829..7092
                     /locus_tag="KUM55_RS25950"
                     /old_locus_tag="KUM55_25955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            7082..7381
                     /locus_tag="KUM55_RS25955"
                     /old_locus_tag="KUM55_25960"
     CDS             7082..7381
                     /locus_tag="KUM55_RS25955"
                     /old_locus_tag="KUM55_25960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7443..7820)
                     /locus_tag="KUM55_RS25960"
                     /old_locus_tag="KUM55_25965"
     CDS             complement(7443..7820)
                     /locus_tag="KUM55_RS25960"
                     /old_locus_tag="KUM55_25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            7889..8068
                     /locus_tag="KUM55_RS25965"
                     /old_locus_tag="KUM55_25970"
     CDS             7889..8068
                     /locus_tag="KUM55_RS25965"
                     /old_locus_tag="KUM55_25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8096..8626)
                     /locus_tag="KUM55_RS25970"
                     /old_locus_tag="KUM55_25975"
     CDS             complement(8096..8626)
                     /locus_tag="KUM55_RS25970"
                     /old_locus_tag="KUM55_25975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2834..3412
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8637..9268)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="87.0"
                     /evalue="5.25e-138"
ORIGIN
        1 tcagccgggt tttctccgcg ttccgcgcct gtcgtttcgc gtggctgacg ttgtacagct
       61 ccagcgccat cagtaaatcc ccgcaaaatc atcatccgct ttcggcccgc cgccgtcggc
      121 cgggggccgg aactccgcca ggggatcggt tttcgccagc accgggaaac gctgcaacgg
      181 gatttcccgg cgctcaaccc gcagcggact gctgaacgcc atccgggcca cacccagccc
      241 ggtcatgacc gcgaacccgt ccggcaggtg ttgcagggtg ttcacgtcaa acagcggccg
      301 cgcctccttc tggatgaagc tgtccccgct gatatgctcc acgttccccg cctcccggct
      361 ggcacggcgg cgctccacgt ccaccctgat ttccccggtc tgccccgccg cccactgcgc
      421 ggactccagg ctggccgccc ggtaaaacca gcggatcggc gtgttatcca gtaccgtggt
      481 tttcacaaat tcagcgggca gatcctgccc gcactgcccg aaatcgccca gcgactggtg
      541 cgcgagcaac agattgcagt cccggctgcg cagcgtcccc agtgcgttca gtacatactt
      601 cgacagcaga tatttaattt catcgagcat cacgctgacg tgtggccact cccggaactc
      661 gtcccgcgca atgatgattt gcgcgcagcg ggcaaacagc attttttgca cacggatcac
      721 cgcctcgtcg tccatcgagc cgataacgta caggcagccg ccgccgttga taatccccgc
      781 cacatcgatg ccactgtccg tctgcaggac gctcaggtca gcgacgtttt ccagcctagt
      841 gatcaacccc ttcacgtcct ttttcagggc ctccggcagc gcgtacgccg ccgccagcac
      901 ctgctgaatg tttgccccct gcggaaactg ctccgcaatc agcttcgccg ctttctgctc
      961 gctgatgcgg tagtggtccg ccacgtcgcc ggtctcgccc aggttaagcc cggcaatcag
     1021 caggtttttc agcgcgtact ggtcgagatc gcggaacaga ttcagctgcg ccggtttccc
     1081 cggccgcaga tccagcaggg tgaagtccgg acagtgcgcc cggaagacgt gcggcgccca
     1141 tgcgtcccct ttggggtcga agtacaccac cgcgtccccg aaatcgcgta cgcagcgaat
     1201 gagcgcgttg gtggccatca cgctcttgcc gctcccgggc aggccgagga tctgaatgtt
     1261 ggtcttgcgc caggtatcac gcggaaccag caccggcttg ccctgctcat ccttacccag
     1321 aaacaccgcc tgctcctgcc gggcctgctc cgaggaaaaa tcggccagcg cattaaccgg
     1381 taccggctcc ccgaaatgca ggtggcgcac gtcggtcatc tctcctgtcc gctggctgct
     1441 gcgccggctc tcctgattca gtttcgcgtt caccgcctcg tcagcagccg caaaatgacc
     1501 gtcaagcagc aggaacgcca gcagccgcgc caacacaatc ccgacagcca gcccgcccgc
     1561 gcaccaggca atcccgaaca ggttagccgg ctgcagcagg taataatctg cgtttgcgta
     1621 gaggtccgga cgcaggacgg tttctcccca gctctcgata aacacggcga caaccagccc
     1681 ccccagaagg gccagaccgg ccgcgccggt cagccagtac gcgtagttcc agacatgtcg
     1741 acgtgccctg accatccgcc cgggcgaatc cagcgaggcc agcaccgaaa atggcgctgg
     1801 ccgtttatta tcgcgcttat agcgatcggc atactcccgt cgctccttat gactcagccc
     1861 cacaaaacgc atccgtccgg cccaaagttc agccagaccg aaaaacaggc cgataactag
     1921 gaaagccccg gccagaccgt aatactcctg gtacagccag ctgacccacg gataaatctg
     1981 ccgggccagc tccggaatgt gccacttcca ggcaagcgaa tccgtattaa acatgttctc
     2041 tctccttcag acgatggttt gccggcgtga tgtgcagccc ccgctgcaca agccgcggac
     2101 atgccccaag gcatggcggc ggcggtcacg tccgtggtga ttgcgttcag ctttcaccac
     2161 ggaggccagc ctctttccgc cccatgcgga aaaggcgcag ccccggagga acaacgagca
     2221 cgccccagcg tgtgagagga aaaggcggca ccccagtgcg gtcttttccg gtgttccggc
     2281 ccccgtcaga gttcgccata gcgagcggct gaccacccgc aaaatcagga gcagctttgc
     2341 tgcttcgcca ggtgcgtcca gcaccgtgga tttttgtggg tggtcaggcg tgagcctgac
     2401 gggggccggg tgaatttctg gcgtggccgg cgtccagccg gacggagcca gaaagagggg
     2461 gagagaccgt gcgtcggtgg cgtccagcca gcgtaagcag gggatatccc cctgaggtca
     2521 gccacgccct cgcggggatg acctgtattt ctccgtaagg agaaaaaagc aacagagata
     2581 ggttaacgta aaatgttcag cgaaaaaccg atatgattca cctatcctgg taatttatta
     2641 tcagggaata taaagactta atcaggggac aatatgccac atcagcgcgt ccggtgtccg
     2701 tcttgtaggt gccggggaaa ttgcgcctcc tcgctcactc ggtcgctacg ctccggccgt
     2761 gagactgcgg cgggcgctgc ggacacatgc aaagttaccc acagattccg tgaataagca
     2821 ggggactaac atgtgaggca aaacggcaag gccacgcagg tggcgttttt ccataggctc
     2881 cgccctcctg actgagttca cataaacaga cgctttttcg gtgcatctgt gggagccggt
     2941 ggctcaacca tgaatcagtc aggagtggcg aaacccgaca ggacttaaag atcatcaccg
     3001 tttccgatga atcgctccct cttgcgctct cctgttccaa ccctgccgtt tcccggatac
     3061 ctgttccgcc tttctccctt gcgggaagtg tggcgctttc tcatagctca cactctggta
     3121 tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac cccccgttca
     3181 gcccgactgc tgcgccttat ccggtaactg ttcacttgag tccaacccgg aaaagcacgc
     3241 aaaacgccac tggcagcagc cattggtaac tgggagttcg cagaggattt gtttagctaa
     3301 acacgcggtt gctcttgaag tgtgcgccaa agtccggcta cactggaagg acagatttgg
     3361 ttgctgtgct ctgcgaaagc cagttaccac ggttaagcag ttccccaact gacttaacct
     3421 tcgatcaaac cacctcccca ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga
     3481 aaaaaaggat ctcaagaaga tcctttgatc ttttctactg aaccgcgatc cccgtcagtt
     3541 cagaggagga cgatggtgcc accgttccac cctgaacatc ccggatatag tttgcctgac
     3601 atgcgaccag aagaaagcct gaaaaaatca atataaaaag aaaaacaacc tttacctttt
     3661 tcatatacct tccctgtatt taaaaacccc ggtattcatc cctgaacacc ggaagtctgt
     3721 atttcaaatc atccttgata acttttgctt accagttacc atcccggtaa acaaaggata
     3781 caaagtaatc aaatttacta atatcgatta catttttaaa gtgtggctgt aatatcgaaa
     3841 cccatgattt ttcaacatca aaccaaccat tattaatatt atcctttaaa ggcatcccca
     3901 gactttcaat gacagaacca tcatcaccga agtcttttga gtattcacca cctttaaact
     3961 cttcggtttt cttatcaaac caatgaatat gtaatttaag ccccatattt acctcttaaa
     4021 gatatttttt aatgtttcgt tttggatccg gccctttaac ctgcttaccc gtttttggat
     4081 cgaatgcccc gaggtgttcg ccatcactgg cgcggtaccc ttcaagctca ccgtgctggg
     4141 aatcccattc ataaatttta cgctttttat caccatacca tcgggcccgc ttaccaccac
     4201 caccttgttt aggggtttta ggtttaccct ctttcaactc tcccaacccc ttaatatctt
     4261 cagtcttggg atcaggaaaa taatcatggc cgtaatcttt agttccctta cgaggttttt
     4321 tcttttcctc atttaattta ttttctgcat ccttcgcttt tttctccttt tgtttccggc
     4381 tctccatcgc cccggaaagc gcagccttat ccctggataa ggattcattc atttcgttta
     4441 aagatgtctg ggcagctttt aatttctcat tctgattctg aatatcctta tcaagatagc
     4501 gaacctgatc ctgccacccg gagccatatt catgaggcgg gttataagtc acaaaatcat
     4561 ctttatttgc ctctgcctct tttacctttt tatcggcttc ctgaacagca gcccttgcac
     4621 cggggatcgc cttctctgtc gatgcaatgc gactgtttaa ggtcgcaata tttttatctt
     4681 cagcatccag ctcagctttc gctttatcat actctctttt cagcccctct tccgggtgag
     4741 cagcgtccca tgcctgctgg cgacgctttt cttcctccag acgctgtttt acctgtgccg
     4801 gagtaagaac atccgtcacc gacacataga ccggtttctg tccgctctct ttcgggaaac
     4861 gaataacggc ctcacgggag ttaccaccgg ccgtaaaacc agccggacgt gaatcacctt
     4921 tttcagcaat aatgcctttt ggcggggctt tcgcggccgg aacaccttta ggtacgctca
     4981 cctgcagaga cgggagaccc ggaatcgaca cactgaatac ccccggacgt tttgtcggtt
     5041 tcgcatccac cacaggaaca ctcattggcc ggccggtgac aaccgcaata tgctgccgct
     5101 cgtccttcac aatatccgcc acacgtttag tgacgctgac cgtcgcctgg tccagcggca
     5161 gggagctgac cggcgtctcc gttaccgtgt ctgccggcaa tgacgtcacg atttttgaca
     5221 tcattttcgg atcatctttt gctatctcag aaggcagcac tccgtaaatc gcaatccccc
     5281 acagaccaaa cttaaacgga cctttcaggg cagccagcac atcagcaaca gcggccgaca
     5341 acgcatcgcc ggaaacggat aaagccagtc cttcagcccc cggagtggcc agggcaggca
     5401 gaccgaaggc catcgcggtg gccgaagact gtccgccacc agagctcccg ctatgattgc
     5461 cgctgttacc gccattacca ccgggtttac tcccgccgtt accaaactcc cccgggttat
     5521 agttatgaat atccccgtta ggtgtatgcg tcgtacccca gcccggcccg ctgtttggat
     5581 ccgtaccacc acctgatgaa gggccacctt tacctgatgt accgttcaca ttcccactgg
     5641 cctgaccacc attgtgtccc agacctgaat tacccggacc tcgtccgtct ccgccactca
     5701 tatacttttc ctctttgact tttaaaacaa taaactaaaa taaaatactg tacatataac
     5761 cactggtttt atatacagta caaaaattac gctgccttgt ttttcatgtc aagtctgtaa
     5821 atttgcgttt ttgtgaaaat gatcaaaaaa acagccactc acaccatgcg caatcccctc
     5881 cagatgcaac aaaaaccagc acaaacggcg ccgttctccg gcctcagtat gttttactga
     5941 aatcattata tgagatacca gaaagccccc tccgggacag gaaaagaaac ggggggtctg
     6001 gttcgggttg ttcggtcact gaacacggtc agaaactgat gataagatag cctgcggata
     6061 tataactcaa tgagaaaaaa gaattaaagg taaggagacg ctcatgagac gttccggcag
     6121 caaactgtgt cttatcgcca tgataacagc gacagcaact ctcactgcct gtgctccaaa
     6181 gggcagcgtg gaacagcata cccggcatta cgtatatgct tcagatgacg gttttgatcc
     6241 caatttttcc acccagaaag ctgacacaac acgaatgatg gtgccttttt tccggcagtt
     6301 ctgggatatg ggcgcaaagg ataaagcgac cggaaaatca cggagtgatg tgcagcaacg
     6361 cattcagcag tttcacagtc aggaattttt aaactcactc caaggcacaa cacaatttgc
     6421 gggtaccgat taccgcagca aagacctcac accgaaaaaa tcaaggctac tggctgacac
     6481 catttcagct gtttatctcg atggctatga aggcagacag taagtggatt taccataacg
     6541 cgtcattacg cgcaccgctc agacgggctc agggcgtttc cggcggctac gggataaaac
     6601 aggcaacaac ccccccggaa aactgccgga aacgcgcccg ataaatttta tcctaatgca
     6661 tgcctatgca cgcaactgca tggcatatgt tttgacgtaa aatggatgcc gtaagccctt
     6721 ttttaccgac tttctgcttc atcagcctga ctgacacgac catattcttt cagaccgccc
     6781 cctgctaaac ttccttcata ttgccgcatt aacccgggag gctccgcaat gagcaacact
     6841 acacactacg aaaacgccaa ctttcttcgg gaactggctg aaagcctgcc ccgcatactg
     6901 ccggaaggtg gcccggacaa agctgcgctg ttgcagcgac tggctaacga agaactggcc
     6961 caggcagagt atgaagatca ggttcgcgcc aaagtgaccg ccgcccgtgc tgacacgcgt
     7021 ccgggtatga ccacggaaca actccgccag cgcctgcatg gccgttatca ggaactgcgc
     7081 gatgccgtat gacgtggaat ggaagcaggg agccatcgag gatgtaacga cgctgtttga
     7141 ctatatcgcg gaaaattcat ccctgtggga tgcgcagaac gttaccgacc gggttctcgc
     7201 tgcggcggac aaactggccg acttcccgcg actctatgaa gtggactcac gctatggcga
     7261 ggacgttcgc cggatcagtc tgatgggaca gcatgtcctc tatgatgttg atgaccagac
     7321 cagaaaagtc cgggttctgg ccgtggtcgg ccagcgccag aatccccata ttgttctcta
     7381 ataaccggtg acggggacgc aattcattgc gtggccgtcg ccggttggac aagcacagcg
     7441 cgtcagcgtt ttccccacgg ccaccaccgg cgcggtgttc cggccggggt ctctgctgtc
     7501 tccggtttgt gttccagcag tctcatggac tgcatttcct gccgtagccc ggccacctcc
     7561 tccctgagtg cttttatctc cgcgatcaac gcgtcggagg ccattttttc agtctcaccc
     7621 gacagtctca actcgtcccg ctcgtgagtc tcatcctgtc ttaactcacc gtacacccgg
     7681 ataagctcgc tggtatccac cacccggccg ccatcagctt ccgtacggta tgatacgcgg
     7741 cctttcgcca tatcccgata cagggaactt cgggctttac ccgtcagttc cgtcgcctgt
     7801 ctcaccgtca cccacgccat tgtctcaccc cgctattttt gagacagtat acctgacagg
     7861 cgcacaccat gcccggaagg aaagaatcat gacaaaacag gaaaaaacag ccctcaatat
     7921 ggctaaattt attaaggagc agtcgctgct attgctcgat aaactgaatg agctggatat
     7981 ggataccccg gccgacatgt gcgagcggct acacgaggat gcggaaacac tctggcaggt
     8041 gatgcgggat aagctcgggg aggaataaca gcaccggcat ggccggtgct ggcatttatt
     8101 ttttgccgaa cggccaccac cggcgggcag gtttgtcctc cggcagttca ggtaccggca
     8161 cgttaaaata gcggctgtta tattcccgca tcagctccgc tttcagcttc tcgttcaccg
     8221 cgctttcccc gtgccgctca ctcaccacgt caatcatcgc gttaatctgc tccggtgagc
     8281 acttcccggc atcaaccacc tgcacaccgt tctcgatggc gaaccgctcc accttgcgat
     8341 agaggtaatc acacagcttc attcctgtgg gtttgttccg ttcccattgc ggatttttac
     8401 cagtgaaaaa gtacgtggct ttttcgtcgc tgttgagccg ctcgtcatac atcccgatgg
     8461 atttcatcat cgccagatgc tgggcccggt cgacaaaaca gaaaacatac gccccggtct
     8521 gccaggcgag caccaccata tcttccagat gctcgctggt cggcatgatg cagcgctcgc
     8581 cgttccggtc ctccgcgtct tcaataaata ccatgtacag cgccataatc cctcactccg
     8641 ttgtcataaa tttaaagcac gcaaacaacc gttcacgatc cagctctgcc gttttgcggg
     8701 cctcgccggt ctgtgggttc acgatttcat ccaccgacag gattgcccgc tgcaccctga
     8761 cctgcgtcgc ccggtccggg cagatgtacc agatttcatc gatgccgtta gctttgcggt
     8821 tgaacagata cccggacacc acttcctgat accggcgacg gctcttgacg gtgcgctcca
     8881 cctcaaacgc caccgtttta ccgtccagct ccgccagggc gtccggcact ttcatgcctt
     8941 taagactcag acggtgcaga atgcgccagc cggtggcccc tctggcttcc atatcaaggc
     9001 gcagggattg cacggccagc cggtgcggca gatgtgctgc cgacaccctt gacggctgaa
     9061 acggaataac atccgccagc ggttcgtcct cgtcgaacga gaacgccacg ccatgcggcg
     9121 tcagccccca cacgctgacc tgacggccgt ccaccggata tttggtccgg accagcagcc
     9181 ctttcgccac caggctgtcg aggaagcggt agaccggctg caccgccgca atccccagcc
     9241 gccgcgcaac cagctccgcc gtggaacaca gctcttcccg caaccagcgc aggg
//
