LOCUS       NZ_CP077775            11972 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain CDI694 plasmid pCDI694-12.0, complete
            sequence.
ACCESSION   NZ_CP077775
VERSION     NZ_CP077775.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19925007
            Assembly: GCF_019107705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Chen,L.
  TITLE     Whole-genome sequencing of two K. pneumoniae isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11972)
  AUTHORS   Chen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 111 Ideation Way, Nutley, NJ 07110,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:27:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,977
            CDSs (total)                      :: 5,855
            Genes (coding)                    :: 5,658
            CDSs (with protein)               :: 5,658
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 95 of 197
            Pseudo Genes (incomplete)         :: 113 of 197
            Pseudo Genes (internal stop)      :: 24 of 197
            Pseudo Genes (multiple problems)  :: 34 of 197
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077775.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CDI694"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCDI694-12.0"
     gene            complement(join(11257..11972,1..16))
                     /gene="mobC"
                     /locus_tag="KUM43_RS27055"
                     /old_locus_tag="KUM43_27050"
     CDS             complement(join(11257..11972,1..16))
                     /gene="mobC"
                     /locus_tag="KUM43_RS27055"
                     /old_locus_tag="KUM43_27050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(16..1980)
                     /locus_tag="KUM43_RS27060"
                     /old_locus_tag="KUM43_27055"
     CDS             complement(16..1980)
                     /locus_tag="KUM43_RS27060"
                     /old_locus_tag="KUM43_27055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3466..3615)
                     /locus_tag="KUM43_RS27065"
                     /old_locus_tag="KUM43_27060"
     CDS             complement(3466..3615)
                     /locus_tag="KUM43_RS27065"
                     /old_locus_tag="KUM43_27060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3700..3957)
                     /locus_tag="KUM43_RS27070"
                     /old_locus_tag="KUM43_27065"
     CDS             complement(3700..3957)
                     /locus_tag="KUM43_RS27070"
                     /old_locus_tag="KUM43_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3967..5652)
                     /locus_tag="KUM43_RS27075"
                     /old_locus_tag="KUM43_27070"
     CDS             complement(3967..5652)
                     /locus_tag="KUM43_RS27075"
                     /old_locus_tag="KUM43_27070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(5757..7244)
                     /gene="ltrA"
                     /locus_tag="KUM43_RS27080"
                     /old_locus_tag="KUM43_27075"
     CDS             complement(5757..7244)
                     /gene="ltrA"
                     /locus_tag="KUM43_RS27080"
                     /old_locus_tag="KUM43_27075"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7889..8134
                     /locus_tag="KUM43_RS27085"
                     /old_locus_tag="KUM43_27080"
     CDS             7889..8134
                     /locus_tag="KUM43_RS27085"
                     /old_locus_tag="KUM43_27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8408..8779
                     /gene="tolA"
                     /locus_tag="KUM43_RS27090"
                     /old_locus_tag="KUM43_27085"
     CDS             8408..8779
                     /gene="tolA"
                     /locus_tag="KUM43_RS27090"
                     /old_locus_tag="KUM43_27085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8776..9141
                     /locus_tag="KUM43_RS29880"
     CDS             8776..9141
                     /locus_tag="KUM43_RS29880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            9316..9594
                     /locus_tag="KUM43_RS27100"
                     /old_locus_tag="KUM43_27095"
     CDS             9316..9594
                     /locus_tag="KUM43_RS27100"
                     /old_locus_tag="KUM43_27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9582..9896
                     /locus_tag="KUM43_RS27105"
                     /old_locus_tag="KUM43_27100"
     CDS             9582..9896
                     /locus_tag="KUM43_RS27105"
                     /old_locus_tag="KUM43_27100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10060..10488
                     /locus_tag="KUM43_RS27110"
                     /old_locus_tag="KUM43_27105"
     CDS             10060..10488
                     /locus_tag="KUM43_RS27110"
                     /old_locus_tag="KUM43_27105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            10514..10693
                     /locus_tag="KUM43_RS27115"
                     /old_locus_tag="KUM43_27110"
     CDS             10514..10693
                     /locus_tag="KUM43_RS27115"
                     /old_locus_tag="KUM43_27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10720..11250)
                     /locus_tag="KUM43_RS27120"
                     /old_locus_tag="KUM43_27115"
     CDS             complement(10720..11250)
                     /locus_tag="KUM43_RS27120"
                     /old_locus_tag="KUM43_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..10652
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2783..3363
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.333"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11261..11970)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.093"
                     /coverage="98.0"
                     /evalue="1.28e-137"
ORIGIN
        1 actgctccag tgtcatcagt aaagccccgc aaaatcatca tccgcttttg gtccgccgcc
       61 gtcggccgga ggcaggtact ccgccagcgg gtccgttttc gccagcaccg ggaaggtttt
      121 cagcgaaatt tcccggcgct caacccgcag cgggctgctg aacgccagcc gcgccacgcc
      181 cagcccggtc atcaccgcga acccgtcagg caggtgttgc agggtgttca cgtcaaacag
      241 cggccgcgcc tccttctgga tgaagctgtc gccgctgatg tgctccacgt tccccgcctc
      301 ccggctggcc cggcggcgct ccacgtccac ccggatctcc ccggtctggc ccgccgccca
      361 ctgcgcggac tcctggctgg ccgcgcgata gaaccagcgg atcggcgtgt tatccagtac
      421 cgtggttttc acaaaatcag cgggcagatc ctgcccgcac tgaccgaagt cgcccagcga
      481 ctggtgcgcg agcagcaggt tgcagtcccg gctgcgcagc gtccccagcg cgttcagcac
      541 gtacttcgac agcagatatt taatctcatc gagcatgatg ctcgcgtgcg gccacctccg
      601 gaactcatcc cgcgcaatga tgatttgcgc gcagcgggca aacagcatct tctgcacccg
      661 gatcaccgcc tcatcgtcca tcgagccgat gacgtacagg cagccgccgc cgttgattat
      721 ccccgccaca tcgataccgc tgtccgtctg caggacgctc aggtcggcca cgttctccag
      781 cttggtgatc agccccttca cgtccttttt cagggcctcc ggcaacgcgt acgccgccgc
      841 cagcacctgc tgaatgtttg ccccctgcgg gaactgctcc gcaatcagct tcgccgcttt
      901 ctgctcgctg atgcggtagt ggtccgccac gtcgccggtc tcactcaggt taaacccggc
      961 aaccagcaga ttcttcaggg cgtactggtc gagatcgcgg aacagattca gctgcgccgg
     1021 tttccccggc cgcagatcca gcagggtgaa gtccgggcag tgcgcccgga agacgtgcgg
     1081 cgcccacgcg tcccctttgg ggtcgaagta caccacagcg tccccgaaat cgcgtacgca
     1141 gcgaatgagc gcgttggtgg ccatcgcgct cttgccgctc ccgggcaggc cgaggatctg
     1201 aatattggtc ttgcgccagg tatcacgagg gaccagcacc ggctggccct gctcatcctt
     1261 acccagaaac accgcctgct gtttccgggc ctgctccgtg gaaaaatcgg ccagcgcgtt
     1321 aaccggtacc ggctccccga aatgcaggtg gcggacgtct gtcatttctc cggtccgctg
     1381 gctgctgcgc cggctctcct gattcagttt cgcgttcacc gcctcgtcag cggccgcaaa
     1441 atgaccatca agcagcagga acgccagcag ccgcgccaga aggacgccgg cgacaagccc
     1501 tccggcacac caggctatcc cgaacaggtt agccggctgc agcaggtaat aatctgcgtt
     1561 cgcatagagg tccgggcgca ggacggtttc tccccagctc tcgataaaca cggcgacaac
     1621 cagccccccc agaagggcca gaccggcagc gccggtcagc cagtacgcgt agttccagac
     1681 atgtcgccgt gccctgacca tccgcccggg tgaatccagc gaggccagca ccgaaaatgg
     1741 cacagtccgg ttattatcgc gcttatagcg atcggcatac tcccgccgcg ccttatggct
     1801 cagccccaca aaacgcattc ggccggccca gagttcagcc agaccgaaaa acaggccgat
     1861 aaccagaaaa aaaccggcca gactgtaata ctcccggtac agccagctga cccacgggta
     1921 aatctgccgg gccagctccg ggatatgcca ctgccaggca agcgaatccg tattaaacat
     1981 gcaatctctc tccttcagac gacgggttta ccggcgtgat gcgcagcccc cgctgcacaa
     2041 gccgcggaca tgccccaagg catggcggcg gcggtcacgt ccgtggtgat tgcgttcagc
     2101 tttcaccgcg gaggccagcc tctttccgcc ccatgcggaa aaggcgcagc cccggaggaa
     2161 caacgagcac gccccagcgt gtgagaggaa aaggcggcac cccagtgcgg tcttttccgg
     2221 tgttccggcc cccgtcagag ttcgccatag cgagcggctg accacccgca aaatcaggag
     2281 cagctttgct gcttcgtcag gtgcgtccag caccgtggat ttttgtgggt ggtcaggcgt
     2341 gagcctgacg ggggccgggt gaatttctgg cgtggccggc gtccagccgg acagagccag
     2401 aaagagggga aaagaccgtg cgtcggtggc gtccagccag cgtaagcaag gtcttttccc
     2461 ctgaagtcag ccacgcccca gcggggatga cttgcatttc tccgcaagga gaaaaaagca
     2521 acagagatag gttaacgtaa aatgttcagc gaaaaaccga catggttcac ctatcctgat
     2581 aattgatcgt cagggaatag aaagacttaa tcaggggaca atatcccaca tcagcgcgtc
     2641 cggtgaccgt cttgcaggtg ccggggaaat tgcgcctcct cgctcactcg gtcgctacgc
     2701 tccgggcgtg agactgcggc gggcgctgcg gacacataca aagttaccca cagattccgt
     2761 ggataagcag gggactaaca tgtgaggcaa aacggcaggg ccgcgccggt ggcgtttttc
     2821 cataggctcc gccctcctgc cagagttcac ataaacagac gctttttcgg tgcatctgtg
     2881 ggagccgtga ggctcaacca tgaatctgac agtacgggcg aaacccgaca ggacttaaag
     2941 atccccaccg tttccggtag gtcgctccct cttgcgctct cctgttccga ccctgccgtt
     3001 taccggatac ctgttccgcc gttctccctt tcgggaagtg tggcgctttc tcatagctca
     3061 cgctgctggt atctcggctc ggtttaggtc gttcgctcca agctgggctg tatgcacgaa
     3121 ccccccgttc agcccgactg ctgcgccctt tccggtaact atcatcttga gtccaacccg
     3181 gacggacacg caaaaacgcc actggcagca gccattggta actgggagtt cgcagaggat
     3241 ttgtttagct aaacacgcgg ttgctcttga agtgtgcgcc taactgcggc tacactgaaa
     3301 gaacagtttt ggtatctgcg gtctcgtata cccagttacc cggttaagca gttccccaac
     3361 tgacttaacc ttcgatcaaa ccgcctcccc aggcggtttt ttcgtttaca gtgcaagaga
     3421 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact gaaccgcgat
     3481 ccccgtcagt tcagaggagg acgatggtgc caccgttccg ccctgaacat cccggatata
     3541 atttgcctga catgcggcca gaagaaagcc agcaacaatc aatataaaaa gaaaaatagt
     3601 ctttaccttt ttcatatact ttccctgtat ttaaagactc cggcattcat ccctgaacac
     3661 cggaagtcag tatttcaaat catccttgat aattttttgt taccagttac cgtcccgata
     3721 atcaaaagct atataacaat tatccacatc aggttttatt tgatttttaa aaaatggctg
     3781 tagtaaagga acccattctt tcttcaaatc aaactcacca ttattaatat tatcctttaa
     3841 aggcattccc aagctttcaa tgacagaacc atcgtcacca aagtctttag aatactcttc
     3901 ccctttaaac tcttcagttt tcttatcaaa ccatgtcaaa cgtaatttaa gccccatatt
     3961 tacctctcaa agatattttt taatgttccg ttttggatct ggccctttaa cctgcttacc
     4021 cgttttggga tcaaatgcgc ctaggtgttc gccatcactg gcacgatacc cttcaagctc
     4081 accgtgctgg gaatcccatt cataaatttt acgtttttta tcgccatacc atcgggcccg
     4141 cttaccacca ccaccttgtt taggtgtttt aggatcacct cgctttaact ctccaagtcc
     4201 ctttatgtct tctgttttag gtactggatg ataatcatgg ccgtagtctt tagtcccctt
     4261 gcgaggcttg ttcttttcct catttaactt attttctgca tccttcgctt ttttctcctt
     4321 ttgtttccgg ctctccatcg ccccggtaag tgcagcctta tctctggata aggattcatt
     4381 cattgcgttt aaagatgcct gagccgcttt taatttctca ttctgattct gaatatcctt
     4441 atcaagatag cgaacctgat cctgccaccc ggagccatat tcatgaggcg ggttataagt
     4501 cacaaaatca tctttatttg cctctgcctc ttttaccttt ttatcggctt cctgaacagc
     4561 agcccttgca ccggggagcg ccttctctgt cgatgcaatg cggctgttta aggtcgcaat
     4621 atttttatct tcggcatcca gctcagcttt cgctttatca tagtctcttt tcagcccctc
     4681 ttccggatga gcggcgtccc atgcctgctg gcgacgcttt tcttcctcct gacgctgttt
     4741 tacctgtgcc ggggtaagaa catctgtcac cgacacataa accggcttct gtccggtctc
     4801 tttcgggaaa cgaataacgg cctcacggga gttaccaccg gctgtaaaac cagccggacg
     4861 tgaatcacct ttttcagcaa caatgccttt tggcggggct ttcgctgtcg gaacaccttt
     4921 cggtacgctc acctgcagag acgggagacc cggaatcgac acactgaata cccccggacg
     4981 ttttgtcggt ttcgcatcca ccacagggac actcattggc cgacctgcga caaccgcaat
     5041 atgctgccgc tcatccttca ccacatccac aacccgttgt ctgacacgaa ccgtcgcctg
     5101 gtccagtggt aaagtacttg ccggagtctc cgtcaccgca tcggccggta atgacgtcat
     5161 aatttttgac atcattttcg gatcatcttt tgcaatctca gaaggcagca caccgtagat
     5221 cgcaatcccc cacagaccaa acttaaacgg ccccttcagg gcagccagca catcagcaac
     5281 ggcggccgac aacgcatcgc cggaaactga taaagccagt ccttcagccc ccggagtagc
     5341 cagggcaggc agaccgaagg ccatcgcagt ggccgaagac tgtccgccac cagagctacc
     5401 gctatgattg ccgctgttac caccattacc accgggttta ctcccgccgt taccaaactc
     5461 ccccggatta tagttatgaa tatctccatt cggcgtatgc gtcgtacccc agcccggccc
     5521 gctgtttgga tccgtaccac cacctgatga agggccacct ttaccagacg taccgttcac
     5581 attcccactg gcctgaccac cattatgtcc cagacctgaa ttacccggac ctcgaccgtc
     5641 tccaccactc ataccgtttt cctctttgac atttaaaaca ataaattaaa gccaacatgt
     5701 cgcggtaggg atacccgtta ccggataccc cccgcacaga tcccggcgtg cgcgatttac
     5761 gcaccgggct cctgcctcgg gtgtctggcg gtgaaccgct ccacaggcca tggatgaaga
     5821 acccgaaccc ttggtagcca tgcggctgcc agtttgtttg ctttcgtcca ggtcgtatca
     5881 tccttctggc tcctgcgcct gagcgcccgg cgccagaggt ttgttacgtg tgtcctgaac
     5941 ttctgcatgg tggggaagtt gcccggtacc gagtgatagt tcaggtatcc ctgaaccact
     6001 ctcctgagcc attttccctg ttcggggatt gagtaatgcc agcgccttcg cagaccgtct
     6061 ttgatggctt tcagagttgc cgtcatccga tcccggcggg tctttcgtat cagcatgaac
     6121 ctgccgttgc gatctttccc gctgatgtgc gtgaacccga ggaagttgaa cgtttctggt
     6181 ttgccttttc ccctgatggc acggttttcg gcagcgaagc ggccgaactc catcagacgg
     6241 gttttctccg ggtgaaccgt gagtccgaac tccctcagtc tgcgctgcat ggctatacgg
     6301 aagcgccggg catcgtatcg tttgtcgaac ccgatgacga tgtcatcggc gtatctgacc
     6361 attaccacat tgcctgtggc atagcgacgt cgccactgat gcgcccacag atcgaagacg
     6421 tagtggaggt atatgtttgc cagcagcggt gagatgaccg caccctgtgg ggtgccttcc
     6481 tccgttgctc gccattgacc ctcctccgac gtcccggctg tgagccactt acgtatgagc
     6541 ctgattaccc tccggtcgcc gatccgatgc tctgtgaacc tgatcagcca ttcgtggctc
     6601 accctgtcga agaactgact gatgtcggca tccagtaccc agtttacgtt agtgcgtacc
     6661 agccctgtgg ccagtgcgtc cagtgcatcg tgctggcttc gcccgggtct gaacccgtat
     6721 gagaacccca taaagtcgtt ttcatagact gcgttcagga ttttcaccag cgcatactgg
     6781 acgatcttgt cctccagcga ggcgatgccg agcgggcgtt gttttccatc cgcttttggg
     6841 atgtagtgac gcctgccggg ctgcgccctg tagctgccct gatgtagcct ccggtgcaga
     6901 tctgttatgt tgttcttcat gtttccggcg tagtccatcc acctgatgcc atccactccg
     6961 gcggccgctt tcctgctcag ggagaggaat gcggcttcca gtgcttcgac tgtcagcagg
     7021 tggaacaatg ctgtaaaccg ttctttcttc cgctgcttcg cagcttcccg cacgcgtgac
     7081 agcctctgtg acatgctttc ccggctctgt gtccggcgca tgtgtggctg ttccgcgttc
     7141 cccttggccc cgctccttcg ctccactgac tccgctcctt tcgggttgtt cgcctgcttc
     7201 gccgctacta tgagcgagtc cgacttctcc tctccgtaca tcaccggcta tgactcctcg
     7261 tcttcccggt gcgggccatc tccgacactg gcagatggtc agaggggaga tctcccggtt
     7321 cccgcgtaga gatcgtattg acatgccagg gtctcagacc ccgccgggtc catgtggcac
     7381 tcgcagtgtc gcaccctatg atgttgcctt ccgttaacag tacaacgtcg gcacccggta
     7441 atttaatata catttcgtgg ctcaatggct ggcctgtcaa cacccctgtc aacgcttcgc
     7501 cccatacctc gcggtatgca acgcatgact cggggacctt gtggattgct ggtccttcaa
     7561 tggtcgggga ctttcacccc ttgatctcta accggtctcc cggcgcacac tgtacatata
     7621 cacagtataa ttattacctt actcctcccg ttttcaggtg tcaatatatg agattagttc
     7681 tcatcttcag gcacaaaaaa gcgcccggag ccacctgtaa gcagatcgcg tcttttcccg
     7741 aaaatttctc atggcaacaa attttattat ttaagacatt catcacactt acatttcaga
     7801 tggtatagac acatctgaaa gctaactggc atgaggagag gacctgcggt taaactgttc
     7861 gggaaacctg agacgcccgg aggccactat gacgcacctg aaactgactc ccgctgaaga
     7921 aaagcgcctg gcggacgcct ttgtcgctca ggcactggcc atgggcctga aagggaacaa
     7981 aacgctgcca aaggcggtaa aacaacaggt gcgggaaagg gtgcttcgtg ccattcagga
     8041 cgaacgtgaa gccaggcggg cgcaggaaat gcgtcgggag aaatacgagg ctacggaaac
     8101 caccttcacc tggcagccac agcgccgccg gtaaggggta tttaccataa cgtcgcattg
     8161 cccgcaccgc tcagacggtc tgagggcgtt tccggcggct acgggataaa acaggcaaca
     8221 aaccacccgg aaaaccgccg gaaacgcgcc cggtaaattg taacccgctg catgcctatg
     8281 cacgcaactg catggcgggc cgtttggtgt aaaaacgata cgataagccg tgtttatgta
     8341 aatgaacgtg gccgtaaaga gtgacaaata tacatgtaac attttttact gacaggtcat
     8401 caacccgatg cgtttaatac cctttatcct gctggcattg tgcagcccct tactcctgac
     8461 ggcctgtaag cattccacca cccagccgcc gaaaatttat actctggata agtcatctaa
     8521 aatcagtagc tacgctgcgg ccatcaagac cgctgttgaa atgcagatgt ttgaaccaga
     8581 ggcctatgcg ggaaaaatgt gcaccgtgcg gatatccata caacgtgatg gcactctgaa
     8641 cagcgccatt gctgaaggag gagatccgaa actctgtaaa gcagcaatat cagccgttac
     8701 ccacgccaaa attccgccgg caccggatga aaatacctgg caaatattca aaaacgcccc
     8761 tctggatttc agaccatgaa agcttttact ctgacagtgc tggcggccat gttcctcact
     8821 ccggtacatg cgataccact aaaacccgta accacactgc agccaaccta cccggtacag
     8881 gccgcagcaa aacgcatcga aggtcaggtg accgccacgt ttgatattct ggacgatgga
     8941 cgagtggaca acgtgtcgct taccggctgt cacctttttt ttcgggaaac gctgacggca
     9001 atgaggcact ggctatatcc cgcagggcac ccggcaatgc gacaggttat aaattttgaa
     9061 ttcagtctga aaaatgacgg cttacaggat ccgcgaccag tcaccaccca aaagctcaat
     9121 gatgatggtt gccgccagta acaggtcctt taacccgcag gctgcaatag tcctggatga
     9181 tgagtgagcc tgtagcagcc ctgattagtg gaagcgaagc tgaaaaatgg gcagcaaaag
     9241 ccgtatttct ggcaaatagc gccgccacgg attaaagtag tcatgtgtaa tacatatgac
     9301 tacaaagggg cttttatgtc atcagcaaca tccatcaggc tcgatgaaga acttaaggac
     9361 cggctgaaaa cgctggccga tgaccggcac cgctcagcgc acgcgctaat gctggaggca
     9421 atcaccgaat acatcgaccg ggaagagaag cgcagccagt acctgcggga cggacaggcc
     9481 gcatggcagc actatcagga aacgggtctg caccttactg cggaagaagc ggaagcctgg
     9541 ataagtacct ggggaactga gaacgagcag gatgctccgc catgccacag gtaacgattt
     9601 ccgcgctggc acagcgcgat ttacaacgcc ttcaggactt tctcaaaacc aaaaatcggc
     9661 tggcagccag aaaaggaggt gaggtgatcg tccgggctat ccagcaactg aaaacattgc
     9721 cagacattgg ccgcccggtg ccgtttctgc cgctggaata taaggaactg gtgatcgggt
     9781 ttggcgacag tggctatgtg atgctctacc gccacgaccg ggaaatggac cagattgtga
     9841 tcgtcaccgt caggcatcag aaagaatccg ggtatccggg ggcagacagc ctctaataac
     9901 cgatgacggg ggcgcaattc attgcgtggc cgtcgccggt ttgacaagcg cagcgcgtca
     9961 gaaaaccaca tcctgaacgg aaaaacgggt aaaattcagc gatacattta ccgtgagaaa
    10021 ttacacacct ttcacacccg ataaaatcag gggtatacag tggcgaaaac attgctcaac
    10081 ctttcacagg cagcacaagc cgcgggcatt accagaagaa cgctatacaa tcatgtaaaa
    10141 cagggaaagg ttaccgtgtc acgtgacggg aaaaacaacc ctgtcgtgga tgtgtcagaa
    10201 ctgatccgtg tatacggaaa tgtgaatatc cctgagaaac aaattcccgg catttcacac
    10261 cgtgaaaata cacaaaaaaa tttcccacag gaacagcttc tggcaatgca aaaggaactg
    10321 gccgatctcc ggcaggctgt caccctgatg ctggaagata aaacatcccg ggaagaagaa
    10381 cgccgccagc acgatgatga gcgccggaaa ctccaggccg aagtggacag attgacaaca
    10441 gaactgacac agaagaaaaa acgcttctgg tccggctggt ttagctgaga acttaccgaa
    10501 gggaatggag aacatgacga agcaggaaaa aactgccctc aatatggcta aattcattaa
    10561 ggatcagtcg ctgctgttgc tggaaaagct gaacgagctg gacatggata ccccggccga
    10621 catgtgcgaa cggcttcacg aggatgcgga aacactctgg caggtgatgc gggataagct
    10681 cggggaagaa taacagcacc ggccatgccg gtgctggcgt tattttttgc cgaacggcca
    10741 ccaccggcgg gcgggtttgt cctccggcag ttcaggcacc ggcacgttaa aatagcggct
    10801 gttatattcc cgcatcagct ccgctttcag cttctcgttc accgcgcttt ccccgtgccg
    10861 ctcgctgacc acctcaatca tcgcgttaat ctgctccggt gagcacttcc cggcatcaac
    10921 cacctgcaca ccgttctcga tggcgaaccg ctccaccttt cgatagaggt aatcacacag
    10981 cttcattcct gtgggtttgt cccgttccca ttgcggattt tcaccggtga aaaagtacgt
    11041 ggctttttcg tcgctgttga gccgctcgtc atacatcccg atggatttca tcatcgccag
    11101 atgctgggcc cggtcgacaa aacagaaaac atacgccccg gtctgccagg cgagcaccac
    11161 catgtcttcc agatgctcgc tggttggcat aatgcagcgc tcgccgtctt tgtcttccac
    11221 gtcctcaata aataccatgt acagcgccat aatccctcac tccgttgtca taaatttaaa
    11281 gcacgcgaac agcctctccc ggtccagctc caccgttttg cgggcctcgc cggtctgtgg
    11341 attcacgatt tcatccaccg ccagaatcgc ccgctgtacc cggacaagcg tcggccggtc
    11401 agggcagatg taccagattt catcgatgcc gttcgcccgg cggttgaaca gatacccgga
    11461 caccacttcc tgataccgcc tgcggctctt gacggtgcgc tctacctcaa atgccaccgt
    11521 tctgccgtcc agctccgcca gcgcgtccgg cactttcatc cctttcaggg ccatacggtg
    11581 caggtaccgc cagccggtgg ccccccggcc ctccatggag aggcgcaggg actgcacggc
    11641 cagccggtgc ggcagctgcg ccgccgacac ccttgacggc tgaaacggaa taacgtccgt
    11701 cagcggctcg tcctcgtcga acgagaacgc caccccgtgc ggcgtcagcc cccacacgct
    11761 gacctgacgg ccgtcaaccg ggtatttcgc ccggaccagc agccctttcg ccaccagact
    11821 gtccaggaag cggtaaaccg gctgtaccgc cgcaatcccc agccgtcgcg caaccagctc
    11881 cgccgtggaa cacagctctt cccgcagcca gcgcagggtc agccgtgttt tctccgcgtt
    11941 ccgcgcctgc cgcttcgcgt ggctgacgtt gt
//
