LOCUS       NZ_CP077743            11899 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Staphylococcus aureus strain NL1 plasmid pNL1-02, complete sequence.
ACCESSION   NZ_CP077743
VERSION     NZ_CP077743.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19813410
            Assembly: GCF_020003165.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11899)
  AUTHORS   Sabat,A.J., Bathoorn,E., Akkerboom,V., Chlebowicz,M.A. and
            Friedrich,A.W.
  TITLE     Staphylococcus aureus ST398
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11899)
  AUTHORS   Sabat,A.J., Bathoorn,E., Akkerboom,V., Chlebowicz,M.A. and
            Friedrich,A.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUN-2021) Medical Microbiology, University Medical
            Center Groningen, Hanzeplein 1, Groningen 9700 RB, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: DNASTAR SeqMan NGen v. 17.2.1.61
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 04:09:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,936
            CDSs (total)                      :: 2,854
            Genes (coding)                    :: 2,760
            CDSs (with protein)               :: 2,760
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 47 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 20 of 94
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077743.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11899
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="NL1"
                     /isolation_source="nose"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pNL1-02"
                     /geo_loc_name="Netherlands: Zwolle"
                     /lat_lon="52.51425405 N 6.12458278 E"
                     /collection_date="2021-02-22"
                     /collected_by="University Medical Center Groningen"
     gene            246..1250
                     /locus_tag="KTE72_RS14520"
                     /old_locus_tag="KTE72_14410"
     CDS             246..1250
                     /locus_tag="KTE72_RS14520"
                     /old_locus_tag="KTE72_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_078099054.1"
                     /translation="MQYNTTKCIDENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNVLEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EEVVKEKPTARWLFLTLSTKNAIDGDHLEQSLKHMSKAFNKLKMYTKVKKNLVGFLRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQVEWVDLWQKALQVNYRPVANIKAI
                     KPNQKGDKDIQAAIKETSKYSVKSSDFLTDDDEKNQEIVNDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKTTDEEEKAHSITAIWNFEKQNYYLKDLKR"
                     /name="protein rep"
     gene            complement(1908..2582)
                     /locus_tag="KTE72_RS14525"
                     /old_locus_tag="KTE72_14415"
     CDS             complement(1908..2582)
                     /locus_tag="KTE72_RS14525"
                     /old_locus_tag="KTE72_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_002512555.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TIYRWVQEYAPILYQIWKKKHKKAYYKWRVDETYIKIKGKWCYLYRAIDADGHTLDIWL
                     RKKRDNHSAYAFIKRLIKQFGKPQMIITDQAPSTKVAMSKLIKDFKLNPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSVNTAKNTLKGIECIYGLYKKNRRSLQIYGFSPCYEINHML
                     AS"
                     /name="IS6 family transposase"
     gene            <2639..2755
                     /locus_tag="KTE72_RS14710"
                     /pseudo=""
     CDS             <2639..2755
                     /locus_tag="KTE72_RS14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002501032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     gene            complement(3575..3775)
                     /locus_tag="KTE72_RS14530"
                     /old_locus_tag="KTE72_14420"
     CDS             complement(3575..3775)
                     /locus_tag="KTE72_RS14530"
                     /old_locus_tag="KTE72_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499374.1"
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]; GO:0003723 - RNA binding [Evidence IEA]; GO:0001072 -
                     transcription antitermination factor activity, RNA binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="WP_031795395.1"
                     /translation="MNNGTVKWFNSEKGFGFIERENGNDVFVHFSGIAGEGYKSLEEGQ
                     NVDFDIVDGQRGEQATNVVAI"
                     /name="cold-shock protein"
     gene            4167..4778
                     /gene="cadD"
                     /locus_tag="KTE72_RS14535"
                     /old_locus_tag="KTE72_14425"
     ARG             4168..4775
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="95.12"
                     /coverage="100.0"
     gene            4797..5144
                     /locus_tag="KTE72_RS14540"
                     /old_locus_tag="KTE72_14430"
     CDS             4797..5144
                     /locus_tag="KTE72_RS14540"
                     /old_locus_tag="KTE72_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_002454672.1"
                     /translation="MSYENACDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNDVLDFYKKGKMAYYFIKDDEI
                     REFFSKNQKGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            5380..6222
                     /locus_tag="KTE72_RS14545"
                     /old_locus_tag="KTE72_14435"
     CDS             5380..6222
                     /locus_tag="KTE72_RS14545"
                     /old_locus_tag="KTE72_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417609.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_049948378.1"
                     /translation="MDKYTEKKQRNQVFQNFIERHIGENQMELVRECNTFLSFVADKTL
                     EKKKLYKANPCKNRFCPMCAWRKARKDALGLSLMMQYVKQVEQKEFIFLTLTTPNVRAN
                     DLEDEIKKYNKSFKKMFERKKVKTLSKGYVRKLEITYNEQEDTYNPHFHVLIAVNKSYF
                     KDTKVYVNQKEWLELWRDVTGMPEITQVHVQKIRQNNNKELYEMAKYSGKDSDYLVNQR
                     VFDTFYKSLKGKQVLVYSGLFKEARKKLKNGELDYLKEIDPTEYIYQIFGSVAKSEKI"
                     /name="protein rep"
     gene            complement(6254..6928)
                     /locus_tag="KTE72_RS14550"
                     /old_locus_tag="KTE72_14440"
     CDS             complement(6254..6928)
                     /locus_tag="KTE72_RS14550"
                     /old_locus_tag="KTE72_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_002512555.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TIYRWVQEYAPILYQIWKKKHKKAYYKWRVDETYIKIKGKWCYLYRAIDADGHTLDIWL
                     RKKRDNHSAYAFIKRLIKQFGKPQMIITDQAPSTKVAMSKLIKDFKLNPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSVNTAKNTLKGIECIYGLYKKNRRSLQIYGFSPCYEINHML
                     AS"
                     /name="IS6 family transposase"
     gene            7141..8517
                     /gene="tet(L)"
                     /locus_tag="KTE72_RS14560"
                     /old_locus_tag="KTE72_14450"
     ARG             7142..8514
                     /name="tet(L)"
                     /gene="tet(L)"
                     /gene_name="tetracycline efflux MFS transporter Tet(L)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(9161..9895)
                     /locus_tag="KTE72_RS14565"
                     /old_locus_tag="KTE72_14455"
     CDS             complement(9161..9895)
                     /locus_tag="KTE72_RS14565"
                     /old_locus_tag="KTE72_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="WP_002345002.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHFT
                     FELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNIPY
                     NISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPREYF
                     HPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKYARID
                     DLNNISFEQFLSLFNSYKLFNR"
                     /name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
     gene            10316..11527
                     /gene="mobV"
                     /locus_tag="KTE72_RS14570"
                     /old_locus_tag="KTE72_14460"
     CDS             10316..11527
                     /gene="mobV"
                     /locus_tag="KTE72_RS14570"
                     /old_locus_tag="KTE72_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033179284.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_032494831.1"
                     /translation="MSFVVARMQKMKSGNLVGVGNHNQRNTDNHSNKDIDVERSYLNYD
                     LVNRTENYKRDIEQFINDNKSSSRAVRKDAVLINEWIITSDNQFFKDKDDKEIKDFFEQ
                     SKDYFAEKFGDENIRYAQVHLDETTPHMHLGIVPFNAEKKLSAKTVFNRQALQAVQDEL
                     PKYLNERGFELERGEKGSERKNLTVPEYKKAKDELKEMTTTLEQRKSEVLALSNDKTAT
                     IKKESLDLKDETKTVKVPSGETIGIGKLRHEFMKNEERKTGNVIISKDKLNALIQGYED
                     LYSSNERLKKYAETDLPKEITKVKGKYSELVKEYNDLVRKSNRNIETLEELEKENKSLK
                     NEIKDIYKGFKAYFKDSKQVTTEQVKTLVGGVVDKVKEFVKGGEFEKIHRNETRTRDRD
                     ELSR"
                     /name="mobV"
     ARG             complement(9165..9895)
                     /name="erm(T)"
                     /gene="erm(T)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(9162..9895)
                     /name="ErmT"
                     /gene="ErmT"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.18"
                     /coverage="100.0"
     Typing          247..1250
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.806"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5381..6208
                     /name="rep_cluster_2246"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.004"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          10317..11524
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcagggaat gagtttataa aataaaaaaa gcacctgaaa aggtgtcttt ttttgatggt
       61 tttgaacttg ttctttctta tcttgatact atatagaaat aactcaaaga tataaaaagt
      121 tgctttatcg ttggtatgac agtgttttta tgtatttttg tgcgtgaaag aaaaaattga
      181 cataaaaaag ctccagtaat atgattaagt tacgacgctt aaatcaatac tggaggtctt
      241 tttatatgca atataatact actaaatgta tagacgaaaa tcaagataat aaaacattaa
      301 aagatatgac gaaaagtggg aaacaacgcc catggagaga aaagaagata gataatgtaa
      361 gttatgcaga tatactggaa atcttaaaaa ttaaaaaggc ttttaacgta aaacaatgtg
      421 gtaacgtctt agaattcaag ccaactgatg aaggttattt gaaattacat aagacatggt
      481 tttgtaagtc aaaattatgc ccagtttgta attggaggcg tgctatgaaa aatagttatc
      541 aagctcaaaa agtgattgaa gaagtggtta aagaaaagcc aactgctcgt tggttatttc
      601 ttactttatc gactaaaaat gcaatagacg gtgaccactt agaacaaagt ttaaaacaca
      661 tgtctaaggc ttttaataaa ttaaagatgt atacaaaagt taaaaagaat ttggtgggtt
      721 ttttacgctc aactgaagta acagttaata aaaatgacgg tagttataat caacatatgc
      781 acgttttatt gtgtgttgaa aatgcgtatt tcagaaaaaa agaaaactac attacgcaag
      841 ttgaatgggt tgatttatgg caaaaagcat tacaggttaa ttatcgacca gtagcaaata
      901 ttaaagcgat caaaccaaat caaaaaggcg ataaagatat tcaagcagct atcaaagaaa
      961 cctctaaata ttcagttaag tcatctgatt ttttaactga tgatgacgaa aaaaatcaag
     1021 aaatcgtgaa tgatttggaa aaaggtttat accgaaaacg tatgttgagt tatggtggat
     1081 tgcttaaaca aaaacataaa attttaaatt tagacgatgc cgaagatggc aatttgatta
     1141 atacaagtga cgaagataaa acaacagacg aagaagaaaa agcacattca attacggcaa
     1201 tttggaattt tgaaaaacaa aattattact taaaagattt gaaacgttag cttaaaagct
     1261 agcgtttttt ttatgtcttt ttgcccctgc gggaactata gcgttcaacc ggcatggttg
     1321 cccaattttc atgttaattt ttaaatttgc atgtaactgg gcagtgttta ttttttcttg
     1381 cccattttgc taacttttaa gtttgcatat aaatgggcag tgtctaaaaa attccccact
     1441 ggtcatgaac aaaaaaatga gctaaacaat ttaacttttt aagttatata aattttaggg
     1501 agttccaatc aaattagaaa aatatataaa agagctatat cgtttttctg gcttatgttt
     1561 aaaaagtaca agtgattgta tttaaaatga ctttagcaat ttaagtaata tgtcttttga
     1621 atacaagtta aatgtcagaa agatgtaccc ttattttgct catatttgcg ttttaagaaa
     1681 catatataaa tatacaggta taaaaatata taaatataga gtttgctcta aataccccct
     1741 taaaattcaa aataaaggca tttaaaaatt aagaaaataa aaaagctact agcaaaaaac
     1801 tagtagctta aatcttagtt aaatttctat gtagtattaa tttttaggtt ctgttgcaaa
     1861 gtcagaaaaa atataactaa ttatcatttt aacatggtag tattccctta actggccaac
     1921 atgtgattaa tttcgtagca cggcgaaaat ccgtagatct gaagagacct acggttcttt
     1981 ttatatagac cataaataca ttcaatacct ttgagtgtat tctttgctgt attgacgctt
     2041 tgatatctcg tctttctcac tttaatatga cggtgatcct gctcaatgag gttattaaga
     2101 tatttagatg tacaatgaca gtcgggatta agtttaaaat ctttaatcaa cttagacatt
     2161 gccaccttcg tcgaaggtgc ctggtctgta attatcattt gaggtttacc aaattgttta
     2221 ataagtcgct taataaacgc atatgctgaa tgattatctc gtttcttacg taaccaaata
     2281 tccaatgtat gaccatctgc atcaatggca cgatataaat agcaccattt tccttttatt
     2341 ttaatgtatg tctcatcaac gcgccattta taataagctt ttttatgctt tttcttccaa
     2401 atttgataca aaataggagc atattcttga acccaacggt agatcgttga atgatgaacg
     2461 ttaacgccgc gttctcttag tatttcagat atatcacgat aactaagcgc gtatcttaag
     2521 taatagccaa cggctacagt tattacatct ttattaaatt gcttatatct aaaataattc
     2581 atagagaacg ctcctttttt gttaaaatta tactataaaa ttaactttgc aacagaacca
     2641 aattttctat atttggaata aaaaagagta cttagccagt gaaatatatg atttaagtga
     2701 acaagaaaaa aaagagatta atcatcaaat gataaacgaa attgaagaag agtaaaaagg
     2761 gcgttagcaa tagctagcgt cttttttatt ccaattgcct tatggtttgg aaccaacaag
     2821 gcttaatgtg ttgttgagtt ggtacttgtt tgggattaat cccaaatcaa gaccaaccaa
     2881 ctcgctagac taaatctagc taacacaaag cctattccaa accataagtt ttctcggcat
     2941 aaaagtgagc atgattttaa attgaaatag tgcgtgattt ttataaagtg caaacccttt
     3001 tcgataaccc ccttttcagt caaaaggggt agtgcaggat taagagttgg tagccaactc
     3061 gtgaaaagcg ttggcacgct tggctttaga gtgggatcat tataactttt ttatgggata
     3121 tatagtattg gaggccttga tatgactggc ttgagcatgg gatattgcat gtaaaatcga
     3181 aaacttggtt tgcggaaact ttgcatttaa ctttgcaata gatttgttgt tgtattcggc
     3241 tcatatttgc gttttaagag gatgtatgaa cttttaggta taaaacccat acaatttacc
     3301 tctaagcctt caaaataccc tttaaaattc aaaataagga cataaaaaat aaaagctacc
     3361 ccagataaaa tgaagtagct ttttctttca tacggtctgt ttatttttct ttcgacgccg
     3421 tatcgtcgat tgaattcaaa aaagttatgt gctataaatc tgcatcactt tttcaatatg
     3481 atatttgaac gttcatataa tgatatctct gttacttttt aaaagaaatt cgccataaaa
     3541 aaacccagtc aatgattgac tgggtttttt atatttagat tgcaacaaca ttagttgctt
     3601 gttcgccacg ttgaccgtca acaatgtcga aatctacgtt ttgaccttct tctaaagatt
     3661 tataaccttc accagcaatg ccagagaagt gaacgaaaac atcgttgcca ttttctcttt
     3721 cgataaagcc aaaacctttt tctgaattaa accattttac tgtaccgtta ttcatataga
     3781 atacctccga gtgcttttgc acataatatt tgtaacaact tcatttataa aaaagaggat
     3841 attctaaaaa atacactaca attaaattca cgagctttat tacgttagat taagtatacg
     3901 cttatttctt tgttaagtac agctttaatt ttaatagcac gcatttatgc cgagaaaatt
     3961 tattgatcaa tgagaagaac ccttaactaa acttgcagac gaatgtcggc atagcgtgag
     4021 ctattaagcc gaccattcga caagttttgg gtttgttaag ggttcagagg ctcgttgtca
     4081 ataaagcaat tggaataaag cacctatgat tatttgaaaa cataagttat aatacattca
     4141 aaaatatttt tgaatgaggt gtaattatga ttcagactat tgtaactgct gctattcttt
     4201 atattgcgac agcattagat ttattagtga ttttattaat gttctttgct agagcaaaga
     4261 ctagaaaaga atatagagat atttatgttg gtcaatattt agggtctatt attttaatat
     4321 tagttagttt gtttttagct tttgtattaa attatgttcc agagaagtgg atattaggtt
     4381 tattgggttt aataccaatt tatcttggaa ttaaagtggc tatttatgat gattgtgaag
     4441 gagaaaagag agctaaaaaa gaattgaatg aaaaaggatt gtctaaatta gttggtacgg
     4501 ttgcaattgt tacgatagca agttgtggtg ctgataatat tggtttattt gttccatatt
     4561 ttgtgacatt aagtgttact aatttattaa ttactttgtt tgtcttttta attttcttct
     4621 tggtatttac tgcacaaaaa ttagctaata ttccaggagt tggagaaatt gttgagaaat
     4681 ttagtcgttg gattatggct gttatttata tagctttagg tttatttatt attattgaaa
     4741 atgacactat tcaaacaatt ttaggattta tattttaatt tagggtgtga ttttatatga
     4801 gttatgaaaa tgcttgtgat gtgatctgtg tacatgagga taaagttaac aatgctttaa
     4861 gttttttaga agatgataaa tctaagaaat tacttaacat tttagaaaag atttgtgatg
     4921 agaagaaatt gaaaattata ttatctttga ttaaagaaga tgagttgtgt gtttgtgata
     4981 tttctttgat attaaaaatg agtgttgctt caacttcaca tcatttaaga cttttatata
     5041 aaaatgatgt acttgatttt tataaaaagg gaaagatggc atattatttt attaaagacg
     5101 atgaaataag agagtttttc tctaaaaatc agaagggttt ttgaaacgag tgaaacgagt
     5161 ttcttctttt cttgataata agggtaacta tttcgatttg aaatagttac ccttaaatcg
     5221 ttggtgtgac tagcttcaag cacaaaaaaa gttgcttttt tcagcatact attgtatcgt
     5281 tttaggtgtc taagctaaac gaaaaataga aaggagaaaa agcaactttt tttatataca
     5341 tagtttgaga aaaactaagt tgtttttgtg tgttataaca tggataagta tactgagaaa
     5401 aaacagagaa atcaagtgtt tcagaatttt atagaacgtc atattgggga aaatcagatg
     5461 gaattagtaa gggaatgtaa tacattttta tcgtttgttg cggataaaac gcttgaaaag
     5521 aaaaaattgt ataaagcgaa tccttgtaag aatcgctttt gtccaatgtg tgcatggcgt
     5581 aaagctcgga aagatgcttt ggggttgtcg ttgatgatgc agtatgtaaa gcaggtagaa
     5641 caaaaagagt ttatcttttt aactttaacg acgccgaatg taagggcaaa tgatcttgaa
     5701 gatgaaatca aaaagtataa taagtctttt aagaaaatgt ttgagcgtaa aaaagtaaag
     5761 acactttcta aaggttacgt tcgtaaatta gagattactt ataatgaaca agaagataca
     5821 tataatccgc attttcacgt gttaattgca gtgaataaat cttatttcaa agatactaaa
     5881 gtttatgtta atcaaaaaga gtggcttgag ttatggcggg atgtaacggg tatgcctgaa
     5941 ataacacaag tacatgtcca gaaaatccga caaaataata ataaagaact ttatgaaatg
     6001 gctaagtatt caggaaaaga tagcgattat ttagtgaatc aaagagtatt cgacactttt
     6061 tataagtcat taaaagggaa acaggtttta gtttattcag gtctttttaa agaggctaga
     6121 aaaaagttaa aaaatggcga gttagattat ttaaaagaaa tcgatccaac agaatacatt
     6181 tatcaaattt tcggttctgt tgcaaagtca gaaaaaatat aactaattat cattttaaca
     6241 tggtagtatt cccttaactg gccaacatgt gattaatttc gtagcacggc gaaaatccgt
     6301 agatctgaag agacctacgg ttctttttat atagaccata aatacattca atacctttga
     6361 gtgtattctt tgctgtattg acgctttgat atctcgtctt tctcacttta atatgacggt
     6421 gatcctgctc aatgaggtta ttaagatatt tagatgtaca atgacagtcg ggattaagtt
     6481 taaaatcttt aatcaactta gacattgcca ccttcgtcga aggtgcctgg tctgtaatta
     6541 tcatttgagg tttaccaaat tgtttaataa gtcgcttaat aaacgcatat gctgaatgat
     6601 tatctcgttt cttacgtaac caaatatcca atgtatgacc atctgcatca atggcacgat
     6661 ataaatagca ccattttcct tttattttaa tgtatgtctc atcaacgcgc catttataat
     6721 aagctttttt atgctttttc ttccaaattt gatacaaaat aggagcatat tcttgaaccc
     6781 aacggtagat cgttgaatga tgaacgttaa cgccgcgttc tcttagtatt tcagatatat
     6841 cacgataact aagcgcgtat cttaagtaat agccaacggc tacagttatt acatctttat
     6901 taaattgctt atatctaaaa taattcatag agaacgctcc ttttttgtta aaattatact
     6961 ataaaattaa ctttgcaaca gaaccattta aaacttagtt tatatgtggt aaaatgtttt
     7021 aatcaagttt aggaggaatt aattatgaag tgtaatgaat gtaacagggt tcaattaaaa
     7081 gagggaagcg tatcattaac cctataaact acgtctgccc tcattattgg agggtgaaat
     7141 gtgaatacat cctattcaca atcgaattta cgacacaacc aaattttaat ttggctttgc
     7201 attttatctt tttttagcgt attaaatgaa atggttttga acgtctcatt acctgatatt
     7261 gcaaatgatt ttaataaacc acctgcgagt acaaactggg tgaacacagc ctttatgtta
     7321 accttttcca ttggaacagc tgtatatgga aagctatctg atcaattagg catcaaaagg
     7381 ttactcctat ttggaattat aataaattgt ttcgggtcgg taattgggtt tgttggccat
     7441 tctttctttt ccttacttat tatggctcgt tttattcaag gggctggtgc agctgcattt
     7501 ccagcactcg taatggttgt agttgcgcgc tatattccaa aggaaaatag gggtaaagca
     7561 tttggtctta ttggatcgat agtagccatg ggagaaggag tcggtccagc gattggtgga
     7621 atgatagccc attatattca ttggtcctat cttctactca ttcctatgat aacaattatc
     7681 actgttccgt ttcttatgaa attattaaag aaagaagtaa ggataaaagg tcattttgat
     7741 atcaaaggaa ttatactaat gtctgtaggc attgtatttt ttatgttgtt tacaacatca
     7801 tatagcattt cttttcttat cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc
     7861 aggaaagtaa cagatccttt tgttgatccc ggattaggga aaaatatacc ttttatgatt
     7921 ggagttcttt gtgggggaat tatatttgga acagtagcag ggtttgtctc tatggttcct
     7981 tatatgatga aagatgttca ccagctaagt actgccgaaa tcggaagtgt aattattttc
     8041 cctggaacaa tgagtgtcat tattttcggc tacattggtg ggatacttgt tgatagaaga
     8101 ggtcctttat acgtgttaaa catcggagtt acatttcttt ctgttagctt tttaactgct
     8161 tcctttcttt tagaaacaac atcatggttc atgacaatta taatcgtatt tgttttaggt
     8221 gggctttcgt tcaccaaaac agttatatca acaattgttt caagtagctt gaaacagcag
     8281 gaagctggtg ctggaatgag tttgcttaac tttaccagct ttttatcaga gggaacaggt
     8341 attgcaattg taggtggttt attatccata cccttacttg atcaaaggtt gttacctatg
     8401 gaagttgatc agtcaactta tctgtatagt aatttgttat tacttttttc aggaatcatt
     8461 gtcattagtt ggctggttac cttgaatgta tataaacatt ctcaaaggga tttctaaatc
     8521 gttaagggat caactttggg agagagttca aaattgatcc tttttttata acaggaattc
     8581 aaatcttttt gttccattaa agggcgcgat tgctgaataa aagatacgag agacctctct
     8641 tgtatctttt ttattttgag tggttttgtc cgttacacta gaaaaccgaa agacaataaa
     8701 aattttattc ttgctgagtc tggctttcgg taagctagac aaaacggaca aaataaaaat
     8761 tggcaagggt ttaaaggtgg agattttttg agtgatcttc tcaaaaaata ctacctgtcc
     8821 cttgctgatt tttaaacgag cacgagagca aaacccccct ttgctgaggt ggcagagggc
     8881 aggttttttt gtttcttttt tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt
     8941 tggtcggcac tgccgacagc ctcgcagagc acacacttta tgaatataaa gtatagtgtg
     9001 ttatacttta catggaagta attgccggaa atagtgaaaa tgcctcacat ttgtgccacc
     9061 taaaaaggag cgatttacat atgagttatg cagtttacgg gccaagattg tttaagcaaa
     9121 ataagataag ttaagggatg cagtttatgc accccttaac ttatctatta aataatttat
     9181 agctattgaa aagagacaag aattgttcaa agctaatatt gtttaaatcg tcaattcttg
     9241 catattttaa ggcttgataa aattggtttt tggaaaatag ttttatgtat tctttgttaa
     9301 cccatttcat aacaaaattt tcatattgtt ttctatcttt gagtgatatt tttgaagggt
     9361 gtctttttaa tacaattaac gagctattaa ctctaggttt tggatgaaag tattctctag
     9421 ggattttact taatatggat atatcaactt ctgtcattaa aaatagtgct agtgaacgat
     9481 ttgtatttag caacctttta gcaaatccat attccactat taaataactt tctgtagctg
     9541 tgctttcaaa aacaattttt cgaattatat ctgtacttat attgtagggt atatttccat
     9601 atatcttata tgacttattt ttaggaaact taaattgtaa aatatcttta ttgataacct
     9661 ggaagttctc atattcaata agtttgtttt tagttatcct acataattta ggatctattt
     9721 caatggcggt tacataatta caccttttag ctaattcaaa cgtgaaatga cctttccctg
     9781 aaccaatctc aataatatta tcatttgtat ttaaatgtac atttctcaaa atttcattta
     9841 tatgatgctt tgaagtaata aagttttgac tatcttttat attttttttg ttcattataa
     9901 cccactccta tgaggttata atgaatgttt atattaacaa tccattataa ccacttattt
     9961 tttgtttggt tgattatgaa attagaataa ttatagcaca agctctgata aataataaca
    10021 tgatgaacga ctgttaaatt tatactgcaa ttgggcccta ttgttgaata aagatacgag
    10081 agatttctct tgtatctttt ttatttttga ttggtaacga aagcatcccc cctttgctga
    10141 ggtggcagag gtcgggtttt tttgtttctt tttttacgaa aaaaagaaag gtcttaaaag
    10201 gttttatggt tttggtcggc actgccgaca gcctcgcaga gtacacactt atatgaatat
    10261 aaagtatagt gtgtatactt tacatggaag ttgtaccgaa gttaggagtg tttatatgtc
    10321 atttgtagtg gctcgtatgc aaaagatgaa atcaggaaat ttggttgggg tgggtaatca
    10381 taatcagaga aatacagaca atcattcgaa caaagatatt gatgttgaac ggtcgtattt
    10441 aaattatgat ttggtcaatc ggacagaaaa ttataaacga gatattgagc aatttattaa
    10501 tgacaacaaa tcaagtagtc gtgctgtcag aaaagatgct gtattaataa acgaatggat
    10561 cataacttct gacaaccaat tttttaaaga taaagacgat aaagaaatta aagacttttt
    10621 cgaacagtcg aaagattact tcgctgaaaa atttggtgat gagaatatcc gctacgctca
    10681 agttcactta gacgaaacaa caccacatat gcacttaggt attgtgcctt tcaatgcgga
    10741 aaagaagtta tcagcaaaga ctgtttttaa tcgtcaagcc ttacaggcgg tacaagacga
    10801 attaccaaaa tatttaaatg agcgtggttt tgagcttgaa cgtggcgaaa aagggtcaga
    10861 gcgtaaaaat ttaacggtgc ctgaatataa aaaggcaaag gacgaattaa aagagatgac
    10921 taccacgctg gaacagcgga agtcagaagt tttagcgtta tcgaacgata aaacagcgac
    10981 tataaaaaaa gagagtttgg atttaaaaga cgaaacgaaa acagtcaaag tacctagtgg
    11041 cgaaaccatt ggtattggta agttgcgaca tgaatttatg aaaaatgaag aacgtaaaac
    11101 aggaaatgtg attatttcta aagataaatt aaatgctttg atacaaggct atgaagattt
    11161 atatagctca aatgagcgac taaagaaata tgcggaaact gatttaccta aagaaatcac
    11221 taaagttaaa gggaaatata gcgaattagt gaaagaatac aatgatttgg tgcgtaagag
    11281 taatcggaat atcgaaacac ttgaagaatt agagaaagag aataaatcgc ttaaaaacga
    11341 aattaaggac atttacaaag ggtttaaagc gtactttaaa gattctaagc aagttacaac
    11401 tgaacaggtt aaaacgcttg ttggtggcgt tgtagacaaa gtaaaagaat ttgtgaaagg
    11461 tggagagttt gaaaaaattc atagaaatga aactcgaaca cgagatagag atgaattaag
    11521 tcgttagaag ctcatatttg cgttctaagg agttttaaat caattcgagt atatttatac
    11581 ctgtttggtc ttttcgtcgg cataagcgtt aaaatttgcg aaaaagaaag cagtacaagg
    11641 gcgaacgtag tgagtgcatt tacccttgtg ctgctttttt cgtaaattag cttttgcaaa
    11701 ccgacagaaa agccaacagg tatgactgtc tactcacttt tcagaggcat gatcaaacgc
    11761 attgcgtttg tgggtgtggg caacgcccac ggttttattg ttttaggaat tgggaaatgg
    11821 actgggggga gattttgaaa aaatcgacca acttctgggg ggtactacga ccgagaggta
    11881 aaaaaagaac gaagtcgag
//
