LOCUS       NZ_CP077435             2944 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella aerogenes strain NTT31XS plasmid pNTT31XS-2k, complete
            sequence.
ACCESSION   NZ_CP077435
VERSION     NZ_CP077435.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19778273
            Assembly: GCF_019048885.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Liu,Z. and Li,R.
  TITLE     Co-occurrence of tigecycline and carbapenem resistance in
            Enterobacter hormaechei and Klebsiella spp. from a porcine
            slaughterhouse
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Liu,Z. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2021) Veterinary, Yangzhou University, 88
            University South Road, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:39:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,139
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,932
            CDSs (with protein)               :: 4,932
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 35 of 88
            Pseudo Genes (incomplete)         :: 57 of 88
            Pseudo Genes (internal stop)      :: 8 of 88
            Pseudo Genes (multiple problems)  :: 11 of 88
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077435.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="NTT31XS"
                     /isolation_source="intestinal contents"
                     /host="porcine"
                     /db_xref="taxon:548"
                     /plasmid="pNTT31XS-2k"
                     /geo_loc_name="China: Yangzhou"
                     /collection_date="2020-07"
     gene            860..1897
                     /locus_tag="KSL85_RS25590"
                     /old_locus_tag="KSL85_25590"
     CDS             860..1897
                     /locus_tag="KSL85_RS25590"
                     /old_locus_tag="KSL85_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RnlA family toxin"
                     /protein_id="WP_085004987.1"
                     /translation="MAQNPFKALNINTDKIEAALTQNGVTNFSSIVRNERETHLSGTYK
                     EIDFLIKLMPSGGNTTIGRASGQNNTYFDEIAGIIKENCLYSDKKTFEYTIPRFSDENK
                     EMLFAFLTEENVKIEEDKNNDPNCKHQYIMTTGNGDRVRAKIYNRGSIQFQGKYLQIAS
                     LINDFMCTILDMKEIIEQKNQEFNVDIKKETIESELHSKLPKSIDLIHDDIKKQLSCSL
                     IMKQIDVEMEDYSTYCFSALRSIEGFIYQLLGSVCNPSSSRNLGEYFIENNPKYIIRDI
                     HQETINGEIAEVLCECYTYWHENRHGLFHMKPGVADTKTIDKAESIEIIDTVCQLIDKG
                     MARLK"
                     /name="type II toxin-antitoxin system RnlA family toxin"
     gene            1897..2277
                     /locus_tag="KSL85_RS25595"
                     /old_locus_tag="KSL85_25595"
     CDS             1897..2277
                     /locus_tag="KSL85_RS25595"
                     /old_locus_tag="KSL85_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RnlB family
                     antitoxin"
                     /protein_id="WP_094315615.1"
                     /translation="MKINKKYQIEVIKDKDKTLFIVYATDIYSPSEFFSKIESDLKKKK
                     VGGDVFFDLIIPNGGKKDRYVHTSFNGERLTSYTLNKVTETDKYKSLSERSASFFKNNF
                     NNINANLLSKATLFALKQGIPI"
                     /name="type II toxin-antitoxin system RnlB family
                     antitoxin"
     Typing          113..591
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.821"
                     /coverage="93.0"
                     /evalue="6.030000000000001e-167"
ORIGIN
        1 ccttgttaat ccaagaacgt caggtgacgt cactttgcgg cgttagccct atcctgcatc
       61 gtattaaaaa atgcagcgat ggcgcgtacg gtggtgaaga taagcccgaa gatattccgc
      121 ttcctcgctc actgactcgc tccgctcggt cgttcagctg cggcgagcgg agggggctgt
      181 cacatcaaac gataaagcga tgaggggcga tagaagggaa ataaaaaggc cgcggcgctg
      241 ccggttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag
      301 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctga tggctccctc
      361 gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt
      421 ttatctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg
      481 tatgcacgaa ccccccgttc agtccgaccg ctgcgccttg tccggtaact atcatcttga
      541 gtccaacccg gaaaggcacg acaaagcgcc actggcagca gccactggta acggattcgc
      601 agaacaaaga agtgttgaag tgtgaggtga agtacagcta cactgtaata acgaatttgg
      661 tttctgtgct ctgcgaaagc cagttacctc ggttcaaaga gttggtagct caacgaacct
      721 tgagaaaacc accgttggta gcggtggttt tttctttatg cgatgatgaa tcaatcagtc
      781 tatcaagtca acgaaccgcg cttccattga caaaaaaaca agatctgcac atattcagaa
      841 aaaacaacgg attgaggcaa tggcacagaa cccatttaaa gcactaaata ttaatacaga
      901 caagattgaa gctgccttga cgcagaatgg cgtcacaaac ttttcttcta tcgtgagaaa
      961 cgaaagagaa acacatttat cagggacata taaagaaata gattttttaa taaagctaat
     1021 gccatcaggt ggaaacacca ctataggaag ggcatcagga caaaacaaca cttactttga
     1081 tgagatcgct ggcattataa aagaaaactg tttgtattca gataaaaaaa cgtttgaata
     1141 cacaatccca agattcagcg atgaaaataa ggaaatgttg tttgcatttc ttactgaaga
     1201 aaacgtaaaa atagaagaag ataaaaacaa cgaccccaac tgtaaacatc aatacatcat
     1261 gaccactggc aatggtgaca gagtcagagc aaaaatttac aaccgtggct caattcaatt
     1321 ccaaggtaaa tatcttcaaa tcgcaagttt gattaatgat ttcatgtgca ccatactgga
     1381 catgaaagag attatagaac agaagaatca agaatttaat gttgacataa aaaaagaaac
     1441 aattgagtca gaattacata gtaaactacc aaaaagcatc gacctaatcc atgacgacat
     1501 caaaaaacag ctatcctgct cgctcataat gaaacaaata gatgtcgaaa tggaagatta
     1561 ctcaacatat tgtttctcag ccttgagatc tatagaaggc tttatatatc aactacttgg
     1621 aagcgtatgc aacccatcat catcaaggaa tcttggcgag tacttcattg agaataaccc
     1681 gaaatacata ataagagata tacaccaaga aactattaat ggtgaaatag cagaagtttt
     1741 gtgtgagtgc tacacttact ggcatgaaaa cagacatggc ttgttccata tgaaaccagg
     1801 tgtggctgac actaagacaa ttgacaaagc agaatctatt gaaattatcg atactgtatg
     1861 tcagttgata gataaaggca tggctaggtt gaaataatga aaataaacaa aaaatatcaa
     1921 atagaggtga taaaggataa agataaaact ttattcattg tctacgctac agatatttat
     1981 agcccgagcg aatttttctc taaaatcgag tctgatttaa agaaaaagaa agttggtggc
     2041 gatgtttttt ttgatttaat aattcctaat ggtggaaaaa aagaccgtta cgtccataca
     2101 tcattcaacg gcgagcgatt gacaagctac acattaaaca aagttacgga aacagataag
     2161 tataaatcct tatcggaacg ctcagcttcg ttctttaaga ataactttaa taatattaat
     2221 gcaaaccttc tatctaaagc cacattattt gctttaaaac aaggtattcc aatataaaag
     2281 caagttcaga tattgaacat aacatccgtt gcgtgcaccg cctgagaggc tctcagggcg
     2341 tttctgcggc ctcctgcctg aaaacgggga ttaacccccg ttaaaaaccg gttttaagcc
     2401 acagttaatt tctgcgcgtg tgcatggcta tgcacacgaa cgcatggccg ccattttagc
     2461 ggctaaaatc ccctgtacgc cctgaaagca atcatgaacc accctgataa cggtgaacgg
     2521 gtgcgcagtt tactgtgcgg ccgtgaggcg ttggccaggc cgtcaggcat ggaaggattt
     2581 attgctaagg gggtcggatt taacgtggat gaatcagcac gcagtgatga acgggattcg
     2641 gaaggggcac ccttgcggcg cggcgatgta tacatcgcta acgtgccttg gtcatttatt
     2701 gcaagaaggg agaggtagca ttttgatagc aaaaaagtag caaaaagata gcaaccgaaa
     2761 gaccagaaga ctctggagat agcaaaaaag tagcaaaatg gtagcaaaaa gatagcaaac
     2821 atgcgggagc taaccaggag tctttagcgg gccggcggta ctccaaaaac acatcttgtc
     2881 ccgctgtttt gtgaataagc cagcatggct gtgggcacaa aacatttcct gtccgctgct
     2941 ttaa
//
