LOCUS       NZ_CP077434             3336 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella aerogenes strain NTT31XS plasmid pNTT31XS-3k, complete
            sequence.
ACCESSION   NZ_CP077434
VERSION     NZ_CP077434.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19778273
            Assembly: GCF_019048885.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Liu,Z. and Li,R.
  TITLE     Co-occurrence of tigecycline and carbapenem resistance in
            Enterobacter hormaechei and Klebsiella spp. from a porcine
            slaughterhouse
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Liu,Z. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2021) Veterinary, Yangzhou University, 88
            University South Road, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:39:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,139
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,932
            CDSs (with protein)               :: 4,932
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 35 of 88
            Pseudo Genes (incomplete)         :: 57 of 88
            Pseudo Genes (internal stop)      :: 8 of 88
            Pseudo Genes (multiple problems)  :: 11 of 88
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077434.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="NTT31XS"
                     /isolation_source="intestinal contents"
                     /host="porcine"
                     /db_xref="taxon:548"
                     /plasmid="pNTT31XS-3k"
                     /geo_loc_name="China: Yangzhou"
                     /collection_date="2020-07"
     gene            550..681
                     /locus_tag="KSL85_RS25910"
     CDS             550..681
                     /locus_tag="KSL85_RS25910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023224512.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDKAPLAAATGNRISRAS"
                     /name="hypothetical protein"
     gene            1201..2136
                     /locus_tag="KSL85_RS25575"
                     /old_locus_tag="KSL85_25575"
     CDS             1201..2136
                     /locus_tag="KSL85_RS25575"
                     /old_locus_tag="KSL85_25575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_171878940.1"
                     /translation="MTAQDVINVLTILKANDSTSFSKIQRALKMSISQLEGIIDGLTAM
                     GIVYKSSFTSYSLTELTSKPVVSDGVRKAFEDIITNRGTYLSEELLQKVSTPFIPLMTH
                     EYKNAPVKVMIVGQETLGMEDAFSTIVSVDDYINESIESFNKFNFGEDLRNSHFWYAFD
                     EVVKYFNLPSRRHAYWTNLHKFQLIENDGDSVSISKLPSKDIMTMIHMQRELFLAEIKD
                     TKPDIIIYFTGGQTWVLDHYLNNGKKLAVKAIDERSHLGIIQTEFLHCPIAICTDHPAR
                     RGYTQAIVDHRANLLKYAADKFYASESARV"
                     /name="hypothetical protein"
     gene            <2166..2269
                     /locus_tag="KSL85_RS25580"
                     /old_locus_tag="KSL85_25580"
                     /pseudo=""
     CDS             <2166..2269
                     /locus_tag="KSL85_RS25580"
                     /old_locus_tag="KSL85_25580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558297.1"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(2329..2787)
                     /locus_tag="KSL85_RS25585"
                     /old_locus_tag="KSL85_25585"
     CDS             complement(2329..2787)
                     /locus_tag="KSL85_RS25585"
                     /old_locus_tag="KSL85_25585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_171878937.1"
                     /translation="MTKKSVPHCIVHACMNGLFFLTTELHRSFSSYITFTTLLHHLYIT
                     FTSFLQLIRHFHPLCHLYITPFLNVLTAGFPAAPATLHGHAINCVPVHRFRMGRTPETG
                     LPLAEWCEFPRYALARILMHAVKTEPARFWRVFGWFVACFTGFPSETP"
                     /name="hypothetical protein"
     Typing          170..659
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.13"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 aaaacgcctg gtcagggcac agccccgaca ccccagagcc gactgtaggt cactgggcga
       61 acgcggcgga atgatgtcgg atgacgtcat tttgcggcgt tcgccctatc ctgcatcgta
      121 ttaaaaaatg cagctatggc gcgtacggtg ccaaagataa gcccggagat attccgcttc
      181 ctcgctcact gactcgctcc gctcggtcgt tcggctgcgg cgagcggtac cggcttactg
      241 gcggggcgga aatttcctgg catcttccag gaagaagctt aacagggaag caagaaggcc
      301 acggcgaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
      361 tcaaatcagt ggtggcgaaa cccgacagga cttaaagata ccaggcgttt ccccctggtg
      421 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
      481 ggccgcgttt atctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
      541 ctggactgta tgcacgaacc ccccgttcag tccgactgct gcgccttatc cggtaactat
      601 cgtcttgagt ccaacccgga aagacacgac aaagcgccac tggcagcagc cactggtaac
      661 aggattagca gagcgagtta atgtaggtgg tgctatagag ttcttgaagt ggtggcctaa
      721 ctacggctac actggaagga cagtatttgg tatctgcgct ctgctgaagc cagttaccag
      781 gttaagcagt tccccaactg acttaacctt cgaaaaacca cctccccagg tggttttttc
      841 gtttcttgga gaagagacta cgacgacaaa tgacggatct caagaagatc cttttaacgt
      901 acaaaaattt ccgttctcca aactacccac caggataact taccaatcat aaaaatcatg
      961 ataggccact acggtcgatg tcttgaagca atgctcggtt atagtcgttt atctctgagg
     1021 atttttagct tgtaaccaat caggcaaagt cgttattgat gccggttcat tagagaaata
     1081 taggggccaa tccagggatt accggggcat gtgatttttg actaattttt tgtactgcga
     1141 ccgtcagaat gagttaaacg cgtatctaca ttgaagcagt tcaatatgag gaaaacagga
     1201 atgacagcac aagatgtaat caacgttctc actattctta aggccaacga ctcaaccagt
     1261 tttagcaaga ttcaacgcgc gttaaagatg agtatttcgc agttggaggg catcattgat
     1321 gggttaactg caatgggaat agtgtataaa tcatcattca cttcatacag ccttactgag
     1381 ttaacctcta aacctgttgt tagtgacggt gtaagaaagg cattcgaaga tatcattacc
     1441 aatcgcggta cttacctgtc agaagaacta ctacaaaagg taagtacacc attcatccca
     1501 ctgatgacgc acgaatacaa aaatgcccca gttaaagtga tgatcgtggg gcaagaaact
     1561 ctgggaatgg aagatgcttt cagtaccatc gtttcggtcg atgactacat taatgagagc
     1621 attgaatcct tcaataaatt caattttggt gaagatttaa ggaatagcca cttttggtat
     1681 gcatttgatg aagtagtaaa gtattttaac ctaccatcca gaaggcatgc ttattggact
     1741 aatcttcata agtttcaatt aattgaaaat gatggagata gtgtcagcat ttccaaacta
     1801 ccttcaaaag atatcatgac aatgattcat atgcagcgtg aattattctt ggctgaaatt
     1861 aaagatacca agccagatat catcatctac ttcacgggtg ggcaaacctg ggtgttagat
     1921 cattatctaa acaatggtaa aaaattagca gttaaggcaa tagatgaacg ctcgcatctg
     1981 gggattatcc agacagaatt tttgcattgc ccaatcgcta tatgtaccga ccaccctgct
     2041 agaaggggat atactcaagc catcgtagat catagagcta atctgcttaa atatgctgct
     2101 gacaaatttt atgcaagtga atcggcacgg gtttaacaga ccacccagag tcatttaaga
     2161 tcgcttacat cgaaatgttt tataacagta acgtcggcat ggttctagcg aacacatgtc
     2221 accgacagaa tatgaaaacc agtattatca acggctcgga agtgtctaga ttatccgtgg
     2281 cgattcaatt taccataaca tccgttgccc gcaccgcctg agaagctctc agggcgtttc
     2341 tgacgggaaa ccggtaaaac aggcaacaaa ccacccgaaa acccgccaga aacgcgcagg
     2401 ctctgttttt acggcatgca tgagtatgcg cgcaagtgca tagcgcggaa attcacacca
     2461 ttcagccagg ggaagtcctg tttccggcgt tcttcccatc cgaaaacggt gaacggggac
     2521 gcaatttatt gcgtggccgt gaagcgttgc cggcgcagcc ggaaaacccg ctgtcagcac
     2581 gttcaggaag ggggtgatgt acaaatggca cagaggatga aagtgacgta tgagctgaag
     2641 gaatgatgta aaagtgatgt ataaatgatg taaaagtgtt gtaaaagtga tgtatgaact
     2701 gaaggagcga tgtaattctg tagtcaggaa gaataacccg ttcatgcagg catgaacgat
     2761 acagtgagga acgctttttt ttgtcacgat ctccccttaa cctgcgtaaa tcaccctcca
     2821 tggttgatta tttttcaacc tcaaaatggt catacaccta acgcagccgc gattttcatc
     2881 gcaactgcat gaattaaaag aaaaaaaatg aatgtttcgt tttcacgagt aatacccagg
     2941 tattaaaaac ccactgtaat tacatgaaaa taaagaaata aaaggtaata ccggcgtatt
     3001 accacgtacg cgggcaagcg aaaaagctat aacgcttccc ggccgacagg cggcccagaa
     3061 tccacagggg catgcaggat ctcttcagta accttcgcca ttaaatcaaa aaggtcatat
     3121 tcgccgggaa gctgcgtatc agtcgaaccc cgttgctcat catattcaga tggcatgggg
     3181 tgatatccag tagcagagag gttgttagcg ggtttcgggg cgcagccctg aaccagtcac
     3241 gtagcgctag cggagtgtat actggctaaa cttcagagat gccaaggaag ggacaggata
     3301 tgttttgtag caccgccacc acggtggcga tactcc
//
