LOCUS       NZ_CP077410             3223 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Enterobacter asburiae strain FDAARGOS 1432 plasmid unnamed1,
            complete sequence.
ACCESSION   NZ_CP077410
VERSION     NZ_CP077410.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357574
            Assembly: GCF_019048485.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1482.15x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 03:02:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,727
            CDSs (total)                      :: 4,608
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 28 of 77
            Pseudo Genes (incomplete)         :: 45 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            Pseudo Genes (short protein)      :: 1 of 77
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077410.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1432"
                     /culture_collection="DSM:106614"
                     /db_xref="taxon:61645"
                     /plasmid="unnamed1"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            complement(join(3058..3223,1..167))
                     /locus_tag="I6L62_RS00005"
                     /old_locus_tag="I6L62_00005"
     CDS             complement(join(3058..3223,1..167))
                     /locus_tag="I6L62_RS00005"
                     /old_locus_tag="I6L62_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_016807542.1"
                     /translation="MNTYIFLGGAIVAEVIGTIFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(335..550)
                     /locus_tag="I6L62_RS00010"
                     /old_locus_tag="I6L62_00010"
     CDS             complement(335..550)
                     /locus_tag="I6L62_RS00010"
                     /old_locus_tag="I6L62_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(554..1039)
                     /locus_tag="I6L62_RS00015"
                     /old_locus_tag="I6L62_00015"
     CDS             complement(554..1039)
                     /locus_tag="I6L62_RS00015"
                     /old_locus_tag="I6L62_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1074..1778)
                     /locus_tag="I6L62_RS23715"
                     /old_locus_tag="I6L62_00020"
                     /pseudo=""
     CDS             complement(<1074..1778)
                     /locus_tag="I6L62_RS23715"
                     /old_locus_tag="I6L62_00020"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1717..2040)
                     /locus_tag="I6L62_RS00025"
                     /old_locus_tag="I6L62_00025"
     CDS             complement(1717..2040)
                     /locus_tag="I6L62_RS00025"
                     /old_locus_tag="I6L62_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2679..2849
                     /locus_tag="I6L62_RS00030"
                     /old_locus_tag="I6L62_00030"
     CDS             2679..2849
                     /locus_tag="I6L62_RS00030"
                     /old_locus_tag="I6L62_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004193911.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="hypothetical protein"
     Typing          complement(2137..2328)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          2308..2791
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          complement(238..1727)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtaggtatat tttccaatgt ttgggaaagc agataaaagg cagcgcaata acttattgca
       61 gttcctacag acggccacag gcgtgtaaat ccttctgaat atttcataaa gatggtgcct
      121 atgacttcag ccacaatagc ccctcccagg aaaatatatg tattcataaa aatacctcat
      181 gttgaaaaat ttaaatatct ttacataaca actaattaat agaacccggc ttatcatagc
      241 gaaggaccat gatgctgcca ctgcgatcgt tccagctcca gcgctttctg gtgctgcacc
      301 gcccgctcct gctgccggtg agtttctatc gcgttcagcc tttcgacaac cggcgcagct
      361 gctcgctcaa actctgcacc tgcctgctca agaccgtgac gcgctcgttc agcgccgcgt
      421 tctcccgttg aataagacca gacatcgtcc gccattccgc gaaggcgttc tcccactcgt
      481 ccagcctttt cgagtagtcc tgctgtagct gctctaatgc gctcagcaac tgtttttcca
      541 gctccgtcat gtgctatttc cccgccagta tcatccagct cgagtcctct ccgaactgtc
      601 ccgcttccgg gttcaccctc acgcagcggc gtctctgctc gccgcaggtc gagagctgta
      661 cgccgctgtt cctctctgac agcatggcct gcgtgctctt ctgctcccgg atggtcgtat
      721 agttctcgag tatctgctgc ccctgccacc acagaatgcc cgcgctcgac gctatcagca
      781 gcgcggagac cagaacgatg gtcagccacg tctggctgac catgcgcagc atccctttcg
      841 tgcgctggct catggctgag gacagcttcc ggtcgtggtc gaggatggcg gcgctgattc
      901 tcttctcgct ttcgctcagt tccgctctga cagacttctc gtgctcgctg aatgcggctt
      961 tcagcatctc gccggtagtc tgctgctgcg ctttcgattt ctgctctaag tccttcgcca
     1021 gcgttaaaag actgttcata gatggctccc ttcagtcgga tgtttcgccc cccgtccggg
     1081 tcggcaatgc tgatgctgct ttttgtggtg cgcaccacct caaaacctgc gctttccagc
     1141 gcctcagtga cgtcctgacg cgttttcagc tccccggatg aggcaagggc aagcaggccg
     1201 ctcgtaatcg cctgggcggc ttcctgcttc gcttccggca gcgcggaagg cgagaccagc
     1261 acccgccggt tctccggcgc gttcgggtca tgcagcccca gcctgccgtt cactatggtc
     1321 tgccaggcat cgatgcgcgg acggtcggcg cggtcgtaat acggctgtag ccgcttgccg
     1381 gtcagaagct ccgtgttcgg gatcaggaag ttcagctcca gccgcccctt gtcccggtgc
     1441 tcaacccaca gcacgctgta ctggtcttta tcgagtccgg gcatcagaac ccgctcgaaa
     1501 ctcgccatca gcttttcgcg ctggccgggc ggtaaatcct gttcggcaaa agacagaacg
     1561 ccggaagtgt actttttggc gtagggcgag gcgtcgataa gctcccggac ttcatccggc
     1621 ttcccctgca gaacgctggc gccgtcgcgc tggcgatctt tacccagcag ataatcgacc
     1681 ggcccgccgc cgccgccgcg cccccgagga tgaaacttaa cgatcatcat cagcaccctt
     1741 ttccagtacg gcatgccgca accgctcgag tccggcatcg atggccatca gcgcggcgac
     1801 aatctgcacg cggtcgtgtc cgcttccacc accggcgtta acctggcggg caatctggtt
     1861 aaggttgttc cccatgccgg caagctgacg aagcagcgcc ggcgagatcg acggaagttt
     1921 gccggcgcgt gcgggcttct cgtccaggca cgtctgccgc atccaggccg cgagttgctt
     1981 accctcgcag cgctccagca gacgccggtg ctcgtcctca gtcacccaca tggtgagcat
     2041 tttgctgcgt ttgtctgcca atccggcctc ctgataacgc cgggacggac gggaaatctt
     2101 ttcccggtcg gcacggtgtt ggacaagccg caggcgcggc agtggtttta gcgggtttcg
     2161 gggcgcagcc ctgaaccagt cacgtagcgc tagcggagtg tatactggct aaacatgtta
     2221 tgacggagtg gcaatttatg aaagtgcttc atgtgacagg ggaaaaatgc ggctgccggg
     2281 cgtaagtgca aaatgcaggt ggtgatgaaa tgacgcctcc tcgctcactc ggtcgctacg
     2341 ctcctgccgt gagactgcgg cgggcgttac cagctcacag attacgggat aagcaaatgc
     2401 cgctcgaagc aggaaaggcc aagaaaagga tgcggagttg ccgtttttcc ataggctccg
     2461 cccccctgac gagtatcacg aaaatgacgc tcaaatcagc ggtggcgaaa cccgatgcgg
     2521 cttaaagata ccaggcgttt ccccctggtg gctccctcgt gctcccccgc attcctgcct
     2581 ttcggtttac cggtgtcatt ccgctgttat ggccgcggtt tctcattcca cgcctgaaac
     2641 tcggttccgg gtaggcagtt cgctccagga tggactgtat gcacgaaccc cccgttcagt
     2701 ccgactacca cgcccgttcc ggtaactatc aacttgagtc caacccggaa agacacgaca
     2761 aatcgccagt ggcggtagcc attggtaact gggttcacgg agcagagatt gtagagaatg
     2821 cctacagagt tcttgaagtg aaagcctgag tgcggctaca ctgaaaggac agtttaggta
     2881 tctgcgctcc gtgaaagcca gttaccatcg gaacaaagag ttggtagctc tgaattccgg
     2941 caaacgaacc accgttggta gcggtggttt ttttgtttac agggaaaaag attacgcgca
     3001 gaaagaaagg atctcaagga aatccttttg ctttggccaa tatgcttcgg taaaaagtta
     3061 atgcgcagaa gattttgaaa aaatattaat aaccaaaact ccaacgcaaa taaggaacat
     3121 tcctgcaatt gcgggccaat caagtttttg accgctaacc aaccaaccag ctaggcttat
     3181 tagtacaatt cccgcaccag accaaattgc gtaagcaata ccg
//
