LOCUS       NZ_CP077393             4995 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain FDAARGOS 1435 plasmid unnamed,
            complete sequence.
ACCESSION   NZ_CP077393
VERSION     NZ_CP077393.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357577
            Assembly: GCF_019048625.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 1596.26x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019048625.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:17:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,606
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,436
            CDSs (with protein)               :: 4,436
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 19 of 54
            Pseudo Genes (incomplete)         :: 36 of 54
            Pseudo Genes (internal stop)      :: 10 of 54
            Pseudo Genes (multiple problems)  :: 10 of 54
            Pseudo Genes (short protein)      :: 1 of 54
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077393.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1435"
                     /culture_collection="DSM:46348"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            215..538
                     /locus_tag="I6L65_RS22920"
                     /old_locus_tag="I6L65_22920"
     CDS             215..538
                     /locus_tag="I6L65_RS22920"
                     /old_locus_tag="I6L65_22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_012477689.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            528..>1181
                     /locus_tag="I6L65_RS22925"
                     /old_locus_tag="I6L65_22925"
                     /pseudo=""
     CDS             528..>1181
                     /locus_tag="I6L65_RS22925"
                     /old_locus_tag="I6L65_22925"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1216..1707
                     /locus_tag="I6L65_RS22930"
                     /old_locus_tag="I6L65_22930"
     CDS             1216..1707
                     /locus_tag="I6L65_RS22930"
                     /old_locus_tag="I6L65_22930"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000096688.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1714..1929
                     /locus_tag="I6L65_RS22935"
                     /old_locus_tag="I6L65_22935"
     CDS             1714..1929
                     /locus_tag="I6L65_RS22935"
                     /old_locus_tag="I6L65_22935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029590204.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2416..2643)
                     /locus_tag="I6L65_RS22940"
                     /old_locus_tag="I6L65_22940"
     CDS             complement(2416..2643)
                     /locus_tag="I6L65_RS22940"
                     /old_locus_tag="I6L65_22940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053504437.1"
                     /translation="MGYHYKELTVIELTAEEFELHKEGYSLELLTNMRNEEHTKYWEVC
                     PSCEGKMDPEENQGHEGYCATCWLDNPDWW"
                     /name="hypothetical protein"
     gene            complement(2779..2982)
                     /locus_tag="I6L65_RS22945"
                     /old_locus_tag="I6L65_22945"
     CDS             complement(2779..2982)
                     /locus_tag="I6L65_RS22945"
                     /old_locus_tag="I6L65_22945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017191052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071686691.1"
                     /translation="MAIYVGPHSKVGRNGGSFQECDINTTNHTVTRKYGRYCTIRDNEY
                     APPTSVPGRHWLQIFRTPDNTE"
                     /name="hypothetical protein"
     gene            3424..3756
                     /locus_tag="I6L65_RS22950"
                     /old_locus_tag="I6L65_22950"
     CDS             3424..3756
                     /locus_tag="I6L65_RS22950"
                     /old_locus_tag="I6L65_22950"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_045332894.1"
                     /translation="MSNDENEYTFKPVNAQAIKALKSLPEWVQAAFVHALDSVFYGVDP
                     GMSIAHLTVIGPGVVELRINGRPAYRCVYRSKAPGQVDLLHAFVKTCQGPDKKNMATTA
                     SRYKLL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3772..4080)
                     /locus_tag="I6L65_RS22955"
                     /old_locus_tag="I6L65_22955"
     CDS             complement(3772..4080)
                     /locus_tag="I6L65_RS22955"
                     /old_locus_tag="I6L65_22955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003341209.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_012477691.1"
                     /translation="MKNSFELISTDPVKFNTWSIKSKLIIIIRKLMIDNKWSQSEAAEK
                     LGISQPRVSNMLNGKLDKFSVDTLLEMVFKMGYKLDMDFTPLNTESPLTMVVKKAMV"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(4430..4561)
                     /locus_tag="I6L65_RS23115"
     CDS             complement(4430..4561)
                     /locus_tag="I6L65_RS23115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058645281.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPEKHDLTPLAAATGKGCTRDN"
                     /name="hypothetical protein"
     Typing          2..102
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.039"
                     /coverage="99.0"
                     /evalue="2.11e-42"
     Typing          complement(4431..4957)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          529..2021
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cacatgaagc acttcactga catccgcatt cgtgccaaca tagtaagcca gtatacactc
       61 cgctagcgct acgtgactgg ttcagggctg cgccccgaaa cccgccaaaa gcctgacgcg
      121 cctgcggctt gtccaacccc gcgccgaccg ggaaaggctt tcccgcccgt cccggcgtta
      181 tcaggaggcc ggtttgtcag agaaacggag caagatgctc accatgtggg tgacggagga
      241 cgagcaccgg cggctgctcg agcgctgtga cggcaggcag ctggcggcgt ggatgcggca
      301 gatctgcctg gacgaaaagc cggcacgttc cgggaagctc ccctcgatct cgccggcgct
      361 gctgcgccag cttgccggca tgggcaataa cctgaaccag atagcccgcc gtgttaacgc
      421 cggcggcggc accggccatg acagggtaca gattgtggcg gcgctgatgg ccatcgatgc
      481 cggccttgag cggctgcggc acgccgttct tgagaaagga acggacgatg atcgttaaat
      541 ttcatccgcg cggccgcggc ggtggcgccg ggccggtgga ctatctgctg ggtaaagatc
      601 ggcagcgtga aggcgccacc gtcctgcagg ggaagccgga agaagtccgg gagctgattg
      661 acgcgtcgcc ttacgttaag aaatacacct ccggcgttct gtcgtttgcg gaggccgatc
      721 tgcctcccgg ccagcgggaa aaactgatag ccagcttcga gcgggttctg atgcccggac
      781 tcgataaaga tcagtacagc attctgtggg tggagcacgc ggacaagggg cgtctggagc
      841 tgaactttct gatccccaat accgagctgc tgaccggcag gcggctccag ccttactacg
      901 accgcgccga ccggccgcgc atcgatgcct ggcagactat cgtgaacggc agactggggc
      961 tgcacgaccc gaacgcgccg gaaaaccgcc gggcgctggt cacgccgtcc gggctgccga
     1021 aaacgaagca ggaggccgca gaagccatta cccggggact gctgaccctt gcttcgtccg
     1081 gggagctgaa aagccgtcag gatgtcactg acactcttga aggtgcaggt tttgaggtgg
     1141 tgcgcaccac gaaaagcagt atcagcattg ccgacccgga cggggggcga aacatccgac
     1201 tgaagggagc catctatgag cagtctttta acgctggcga aggacttaga gcagcaatcg
     1261 aaagcgcagc agcagagtac cggcgagatg ctgaaagccg cattcagcga gcacgagcag
     1321 tctgtcagag cggaactgag cgcaagcgcg aggagaatca gcgacgccat cctcgcccac
     1381 gagcagagta tgagcgaggc gatggagaaa aaccggcgga gcgtactgcg gactgccggg
     1441 cggacatggc tcaccatcct gatggtgtca gcgctgctga tcggcacgag cgggagcatt
     1501 ctgtggtggc aggggcagca gataaccgac aattacacgc atcttcgtca gcaggaagat
     1561 accctggcga aaatgaccgc ccggacgtgg ggagtgcgct atcaggagag cagcgacggc
     1621 cggaggtttc ttatcctgcc gccggggatg caaaccgaag ccatccccta cgacgggacg
     1681 acatggatcc gcctgaaaca ggagtagcgg aaaatgacag agctggaaaa gcagttgctg
     1741 agcgcattag agcagctaca gcaggactac tcgcaaaggc tggacgaatg ggagagcgcc
     1801 ttcgcggaat ggcggagcat gtgtgggctt atgcaacggg agaacgcgat gctgaacgag
     1861 cgcgtgtcgg acttgagcac gcaggtgctg agtttaagcg agcagctgcg ccgcttgtcc
     1921 gggaactgaa tgccatcgag ctccggcaga gggagaatga gtatcagaaa acactggagc
     1981 tggagcgcca gccgcagaaa acctaccgcg ggccgacact ttaaaccatc caggcagctg
     2041 acaacccgca cgaagagggg ctcttatttc tcccgctaaa taggttcgtg atcaaaaagg
     2101 tcacgactta ttcaggagcg ggaaatgaag ctgttaaatc ttattgaatc attgcttaac
     2161 tgcctgtatc tggcggcgga gctgtgggac tggctggaga agcatttgtc ggtttttaag
     2221 gcattggcgc ggattttagt gtgactttaa catccatgca tctggctgca tgactatgca
     2281 ccagtgtaat attcagcggc tgcgtttctg gtgttttttc gttatgtctg tcgccacttt
     2341 ctcgcgtctg gcaccggaaa cgcagccaac cccgtttcag cggtgcgggc aacggatgtt
     2401 atggtaaatt attaactacc accagtcagg gttatcgagc cagcatgtgg cacagtaacc
     2461 ttcatgccct tgattttctt ctggatccat ttttccttcg caggaagggc aaacttccca
     2521 atacttggtg tgttcttcat ttctcatgtt agttaaaagc tctaaagagt atccctcttt
     2581 atgaagctca aactcttccg cagttaattc gatgacggtt aattccttgt aatggtaacc
     2641 cattcgcttg cctcgcttta tgcagctcaa tactaagata ctgaaaagca ttaaatgtca
     2701 gtgttttcag tatcttacaa tgataatcct atccggttta tacaagggat attaccgctt
     2761 tactaagcag aagatgaatt actctgtgtt atctggcgtg cggaaaattt gcagccaatg
     2821 gcggcctgga acgctggttg gaggggcgta ctcattatcg cggattgtgc agtagcggcc
     2881 atattttcgg gttaccgtat gattcgttgt gttgatatca cattcctgga atgaaccacc
     2941 attacgacct actttgctgt gaggaccaac ataaatagcc attctaagct ccttcgtcag
     3001 atagttgatt accagtatct aagtagattt tattaatttc tatatttatc aatatcatag
     3061 tgtgctttgt tggctttcca cattgcgtcg ataaaatcct catatcttga tgtaaggaaa
     3121 accaacacat gtaactgtta ggtcttctgc gcctaagtca aattgatttt tttttattca
     3181 aacagacgtt tgaagcacta ataagaattg atgacatata agaatttata tactatggca
     3241 tcgttggcga gattgccaat gaattgttgg ttcaacgagc aataaccatg cgcgcacttt
     3301 cctaaatata gggcggtgtg gtttttttat gctcataaat caatcaatca atcggagttt
     3361 atatgcgaaa cgttttaaca atagttaagt tgctaaaaat caataaccta gatgaggatg
     3421 tttatgtcaa acgacgaaaa tgaatacacg ttcaaacctg ttaatgccca ggctatcaag
     3481 gcattaaaat cactcccaga atgggttcag gctgcattcg tgcacgcatt ggattcggtg
     3541 ttctacgggg tggatccggg gatgtccatc gcacacttga cggtcatcgg ccccggcgtt
     3601 gttgagttga gaatcaatgg tcgcccagct tatcgctgtg tataccgcag taaggcaccc
     3661 ggccaggtgg acttactgca tgcgtttgtg aaaacgtgcc aaggaccaga taagaaaaac
     3721 atggcaacca cagcttcgcg ctacaagttg ctctaatcaa attggcgatg gttacaccat
     3781 cgcctttttt acaaccatag tcaggggcga ctctgtgttt aatggagtga agtccatatc
     3841 cagcttgtac cccatcttga aaaccatttc caacagcgta tcgacggaga atttgtccag
     3901 ttttccgttc agcatattgc ttactcttgg ttgactgata cctaactttt ctgccgcttc
     3961 tgattgcgac catttgttat caatcattag ttttctaatt atgataatga gttttgactt
     4021 gatcgaccat gtattgaact ttactgggtc tgtactgatc agctcaaatg aatttttcat
     4081 agaacagctc cacttcggat tacctgtatc gcaatgataa aagttccggc aaggaactcc
     4141 ttatatatat atacgggcgg ggcattatac aagtaatgat attgctttca agaggaaaaa
     4201 gtgtaaagac attttgatct tttaagtaaa gcttattgtg ttgacgtatc aatccagcaa
     4261 agagtagtct ttgcccataa acgaaaaaac cacccggcaa ggtggttttt cgaaggttca
     4321 ttaaaccggc aagtttatga accgtggtaa cagggtgtac aagaccgctg ccacctaatt
     4381 cgtccttcca gtttagccgt agttggactg atacttcaag aactctgcat cagttatctc
     4441 ttgtacaccc tttaccagtg gctgccgcca gtggcgttaa gtcgtgcttt tccgggttgg
     4501 actcaagtcg atagttaccg gaacaggcgc agcagtcgga ctgaacgggg ggttcgtgca
     4561 tacagtccag cttggagcga actgccttcc cggaaccgag tgtcaggcgt ggaatgagat
     4621 aaacgcggcc ataacagcgg agtgacaccg gtaaaccgaa aggcaggaac aggagagcgc
     4681 acgagggagc cgccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4741 cactgatttg agcgtcagat tttgtgatgc ttgtcagggg ggcggagcct atggaaaaac
     4801 ggcttcgccg cggccttatt gcttccctgc taagtttctt cctggcatct tccaggacat
     4861 ttccgccccg ccagtaagcc atttccgctc gccgcagccg aacgaccgag cgcagcgagt
     4921 cagtgagcga ggaagcggaa tatatcctgt atcgcatatt ctgctgacgc gccggtgccg
     4981 tattttttct ccttc
//
