LOCUS       NZ_CP077377             3065 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter lwoffii strain FDAARGOS 1394 plasmid unnamed8,
            complete sequence.
ACCESSION   NZ_CP077377
VERSION     NZ_CP077377.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357536
            Assembly: GCF_019048525.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.1.1, SPAdes v. 3.14.1
            Genome Coverage                   :: 8261.12x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:39:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,330
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 111
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 38 of 119
            Pseudo Genes (incomplete)         :: 73 of 119
            Pseudo Genes (internal stop)      :: 32 of 119
            Pseudo Genes (multiple problems)  :: 20 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077377.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1394"
                     /culture_collection="DSM:101862"
                     /db_xref="taxon:28090"
                     /plasmid="unnamed8"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            join(2554..3065,1..427)
                     /locus_tag="I6L24_RS16510"
                     /old_locus_tag="I6L24_16515"
     CDS             join(2554..3065,1..427)
                     /locus_tag="I6L24_RS16510"
                     /old_locus_tag="I6L24_16515"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_005080187.1"
                     /translation="MDLDVKKHYPKDWIVLQNRVVECFRGMSLDEKRLFIMATPLARTT
                     KISSNDPIFISSSDFSKECGIDLSTAYTALELASERLFTRFFGYTNAEGDRVKMRWLNK
                     VIYKAGQGGSELYFTDEVLLLLREFDALNPYTKYKKEVVLRLKKDYSLDFYHLAKKHQT
                     MGGFQISLDELFQQLGLPESYQDLSNLKKRVIKPSLDEITANTDIDLSYENVKRGRSVV
                     GFKFTVKEKPKPKVIETGRDPNTPDLFHKMTESQLDTFSSKLSELPEVQKMAHAGEDMK
                     PFIARIRSMLKDSEKQKTLLPHLAKLGFKSK"
                     /name="replication initiation protein"
     gene            424..615
                     /locus_tag="I6L24_RS16515"
                     /old_locus_tag="I6L24_16520"
     CDS             424..615
                     /locus_tag="I6L24_RS16515"
                     /old_locus_tag="I6L24_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005080189.1"
                     /translation="MMFEKKITQFEREVLLQEKIEQLENELKEFSDLQKKAYSERLQKS
                     IVGLENRIYRIKKMLYTT"
                     /name="hypothetical protein"
     gene            767..1900
                     /gene="mobV"
                     /locus_tag="I6L24_RS16520"
                     /old_locus_tag="I6L24_16525"
     CDS             767..1900
                     /gene="mobV"
                     /locus_tag="I6L24_RS16520"
                     /old_locus_tag="I6L24_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080191.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_005080191.1"
                     /translation="MSFAILRIQKLKSFADVGGSLSHNYRNRETLNADDARTHLNEHAL
                     DTNEKCMTAIRDRIPEKRRKDAVLCIEHLITASPEWDGWGTEKETAFFEQSKKWLENKY
                     GKNNVVSTTIHRDETTPHLVAYVVPVDEETGRLNAKKYIGGSRHTLSQMQTDFAVEVKD
                     LGLDRGIQGSKAKHTSIQEYYEKLNNYENEPGIEKGLTYEVPEPEFFESKNAYGERVAN
                     AMIDQIFDQIAPRFDRANALASQTEKLKKELSDTKKTLDEVQQRAKPYLDIINEYNHPN
                     LEKEFNKQVAKLKDNFDSALEHHRFLKRQEEQERFNQQRELRNQLHLEHEQKKQLAEQE
                     KQEKERLALLRRQELEKQRKNEPKKPDNANDNDYSPS"
                     /name="mobV"
     Typing          6..427
                     /name="rep_cluster_1223"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.199"
                     /coverage="99.0"
                     /evalue="2.36e-168"
     Typing          2555..3059
                     /name="rep_cluster_1223"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.465"
                     /coverage="99.0"
                     /evalue="1.9300000000000003e-129"
     Typing          738..1897
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.698"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agacgaatta tttcagcagt tgggcttgcc tgaatcctat caagacctaa gcaacctaaa
       61 aaaacgtgta attaagccat cactggacga gatcacagcc aacaccgaca tagacctaag
      121 ctatgagaat gtgaaacgtg ggcgttccgt ggttggcttt aagttcactg tcaaagaaaa
      181 accaaagccc aaagttatag aaacaggacg agatccaaat acacctgacc tattccacaa
      241 aatgacagaa tctcaacttg atacgtttag cagtaaactt tcagaactcc ctgaagtgca
      301 aaagatggca catgcaggcg aagatatgaa accatttatt gctcgtattc gttcgatgct
      361 caaagactct gaaaaacaaa aaacgttgct tccccatcta gcaaaattag gatttaaatc
      421 gaaatgatgt ttgagaaaaa aatcactcag tttgaaaggg aagttttgct acaagaaaaa
      481 attgagcagc tcgaaaacga gcttaaagaa ttttctgatc ttcaaaaaaa agcatatagc
      541 gaacggcttc aaaaaagtat cgtgggttta gaaaatagaa tctatcgaat caagaaaatg
      601 ctttatacaa cctgaaaggt tgtcaggggt taaggggagg attcccctta ctcatgaact
      661 ttcaatgtac ctttaggtgc tttgaaagtg gagtgagtcc acttcgctat cgcaaagctc
      721 aaaaagctct gctatcgtgt gttttttgcg gtgtcggatt tcgcccatgt catttgctat
      781 tttgcgtatt cagaaactca aatcatttgc cgatgttgga ggcagtcttt cgcataatta
      841 tcgcaatcga gaaacgttaa atgccgatga tgctcgtaca catttgaatg aacatgcctt
      901 ggataccaac gaaaaatgta tgaccgctat tcgtgatcgt attcctgaaa aaagacgtaa
      961 agatgcagtt ttgtgtattg aacatttgat tacagcgagt ccggagtggg atggctgggg
     1021 aactgaaaaa gaaaccgcat tttttgagca gtcgaaaaaa tggcttgaaa ataaatatgg
     1081 taaaaataat gtcgtcagca ccaccattca ccgtgatgaa acaacccctc accttgtcgc
     1141 ctacgttgtt ccagtggacg aagaaacggg gcgattgaat gctaaaaaat atatcggtgg
     1201 atctagacat acactttcac aaatgcagac tgattttgca gttgaagtaa aagatttagg
     1261 actagatcga gggatccaag ggagcaaggc aaaacatacg tctattcaag aatattatga
     1321 aaaattgaac aattacgaga atgaaccagg cattgaaaaa ggactcacct atgaagtgcc
     1381 tgaacctgag ttttttgaat ctaaaaatgc ctacggtgag agagtcgcaa acgctatgat
     1441 tgatcagata tttgatcaaa ttgcgccacg atttgaccga gcgaatgcac tggctagtca
     1501 aacggaaaaa ttaaaaaaag aactgtctga taccaaaaaa acactcgatg aggttcagca
     1561 acgagcaaag ccatatttag acataatcaa cgaatataat catccaaatc ttgagaaaga
     1621 atttaataag caagttgcta aattaaaaga taattttgat tcagcacttg aacatcatag
     1681 attcctaaaa agacaggaag aacaagaaag atttaaccaa caacgtgaac ttcgcaatca
     1741 attacactta gagcacgaac aaaaaaaaca actggctgag caagaaaagc aggaaaaaga
     1801 acgtttggca cttttaagac gtcaagaatt agaaaaacag cgaaaaaatg agcctaaaaa
     1861 acctgataac gccaatgata acgactactc accctcataa cacccattta aacgcaaaaa
     1921 aatggggttt ttagccatgt ttggtgtttt atgcatgaaa gtatagttga gataataaaa
     1981 acctcttaga acgcaaatta gagcgtttta gcgagtgttt acgagcgaca caatgaaaat
     2041 tcgcttattc ccccctctgg aaaaccgctt ttgcttttta aaacagatga atcgcagatg
     2101 tagcaatgct ttcaacgcta tatctatacg tttatcgacc caaagagtta gcgagtgtct
     2161 atgagcggag tattgatgct tttgctctta aaaaagcatg agcatagcga atgcattagt
     2221 aacgcttttg cttttgcttt tgctcttgaa aagacacgag cgaagcgagt gccatagcct
     2281 ttgattttgc ctttgattct cttttttagc ttttaaaaag cttttaaaga cttttcgccc
     2341 ctctacagac cttgctagac atagctttca gactttaaaa aagtacaaat gccaagtatt
     2401 aaagtacaaa cgccaagtta aaagcactta aaaaagtaca aacgccaagt taaaaaagta
     2461 caaacgccaa gtattaaagt acacaattaa actttgaact tttattggtt aaaaggtatt
     2521 gtcttaaacg atgtacttta tttggaaaat actatggatt tggatgttaa aaaacactat
     2581 cccaaagact ggatagtttt acaaaataga gtggttgaat gctttagagg tatgtccctt
     2641 gatgaaaagc gtttatttat catggcaacc cctttggcaa gaaccacaaa aatatcaagc
     2701 aatgatccta tttttatatc ttcgagtgac ttttcaaagg agtgtggcat tgacttatca
     2761 acggcttata cagctttaga attggcatct gaacgcctat ttactcggtt ctttggttac
     2821 accaatgctg agggtgacag ggtaaaaatg cgttggctaa acaaggtgat ttataaggca
     2881 gggcaaggcg gttcagaact atattttaca gatgaagtac ttctattact tagagagttt
     2941 gacgccctaa acccttatac caagtataaa aaagaggtcg ttttacggct taaaaaagac
     3001 tactcgctag acttctatca cttggctaag aaacaccaaa caatgggcgg ttttcaaatc
     3061 agcct
//
