LOCUS       NZ_CP077375             8260 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter lwoffii strain FDAARGOS 1394 plasmid unnamed6,
            complete sequence.
ACCESSION   NZ_CP077375
VERSION     NZ_CP077375.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357536
            Assembly: GCF_019048525.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.1.1, SPAdes v. 3.14.1
            Genome Coverage                   :: 8261.12x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:39:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,330
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 111
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 38 of 119
            Pseudo Genes (incomplete)         :: 73 of 119
            Pseudo Genes (internal stop)      :: 32 of 119
            Pseudo Genes (multiple problems)  :: 20 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077375.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1394"
                     /culture_collection="DSM:101862"
                     /db_xref="taxon:28090"
                     /plasmid="unnamed6"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            290..1477
                     /locus_tag="I6L24_RS16420"
                     /old_locus_tag="I6L24_16425"
     CDS             290..1477
                     /locus_tag="I6L24_RS16420"
                     /old_locus_tag="I6L24_16425"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_127262073.1"
                     /translation="MFYGLENIIDSEEFISDIFNKKSILKKNFSKLHEVFNLIDFDKYL
                     ITMEGNLHNVVRIIKDQKGISIPANVDNIETQRDFVLRQFSEGATLKLEDLDFRNTEIA
                     KMCKTLESIVGGEVFAKPILTGIGKKSFNIHYDMPDIFVIQLEGTKHWKVWPNHVKNPT
                     YSMQEVLVQDNLPDPEIDILLEPGDILYIPGGTPHIARCVDDYSLHMSIGMIPMKIHDV
                     LEGYIKMLSQQLPILRETIYPFSSNSNLEEIKNKIIDQLNCIPIKNMVDDYMLMQNAYK
                     HETSEYRLKSFLLSSKINLKSKLKLKLGSNLNIRKNRDKINVYYSSTIAPIALFKINPT
                     YIEFPEYCEDAIDYLIKMENIEFHPEDILTKLDSDSKIVLCQELLNAGILILVRL"
                     /name="cupin domain-containing protein"
     gene            1488..2300
                     /locus_tag="I6L24_RS16425"
                     /old_locus_tag="I6L24_16430"
     CDS             1488..2300
                     /locus_tag="I6L24_RS16425"
                     /old_locus_tag="I6L24_16430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017702831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="WP_127262074.1"
                     /translation="MYLNLLSKILRNTIYEVNAPTPTPLEMSEIEEILDSLLKEGIDLN
                     ISKDNLVAALQYTKKSRNIHTYVSEKSLDNIKSCVDLIEKDCVPGDLIDCGVLRGGTSI
                     YMAGILKSYGIKNRNLIVADSFEGLPPPCIEDGVFSNEFWYRFSKNLPMYFLDCYCDIE
                     QVKNNFKLYGLLSEQVIFKKGWFSKTLDPKYFKNNLSLIRIDADWYQSTLDVLESCYPK
                     LSKGGIVIIDDYKLVGCRKAVDEFRISNKIFDEIKFADKDAGVIWWRK"
                     /name="TylF/MycF/NovP-related O-methyltransferase"
     gene            2328..3524
                     /locus_tag="I6L24_RS16430"
                     /old_locus_tag="I6L24_16435"
     CDS             2328..3524
                     /locus_tag="I6L24_RS16430"
                     /old_locus_tag="I6L24_16435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="WP_127262075.1"
                     /translation="MKKNKLLLCSEGFSIFGGWIDFIVILSIAAFKFNINSFEISVLSA
                     GILLPSIVFVKYIGVLSNSKNTINYLMISLLIRAIIGIIIIFCESLLIFSFFVVLRATF
                     NSFNLPAIQTIAANNVSETDRNSYYTSLNIVNSISKILAPSLGGIIALVYDSNYSLILS
                     SIFTVISFIIIFCIRNSLNIVQTERKNEKKLSLKTSESERKMWNLVLPIICIFFLFVFM
                     INNQLPVILKDLKMNEASLGLLISSSALGNILYGFYNLKLIKNTSIDSLKKLKNSTFSI
                     MIIFILIGILINSSILSISYLMILFFVSGVFSSKFSIYLNIYLSTYMLENFGQIISKLQ
                     AIQNLIILFAPFLGAFILIKYNPSILFITCGVLGLVSMLILNILIKYSLFKNLNNVET"
                     /name="MFS transporter"
     gene            complement(<3635..>3773)
                     /locus_tag="I6L24_RS16435"
                     /old_locus_tag="I6L24_16440"
                     /pseudo=""
     CDS             complement(<3635..>3773)
                     /locus_tag="I6L24_RS16435"
                     /old_locus_tag="I6L24_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611903.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /name="ISNCY family transposase"
     gene            complement(4039..4596)
                     /locus_tag="I6L24_RS16440"
                     /old_locus_tag="I6L24_16445"
     CDS             complement(4039..4596)
                     /locus_tag="I6L24_RS16440"
                     /old_locus_tag="I6L24_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005402002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_240664393.1"
                     /translation="MNTTKFSVMDASKAFKKSRTTIYEALKNGELSRDHDGLIDLSELI
                     RVYGNPVGVQSSTRTEQVQKDVQVHVQSEVESVLKDQISLLKNQLDLANQREKSLMQHI
                     EDLTHRIEFKGTLEQPKQENINKLNESTNSNIATDPRPQTESNYDELTTPQKDNKRIPV
                     PEHVEPEPKKRGFLSRFFLPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(4657..5607)
                     /gene="repM"
                     /locus_tag="I6L24_RS16445"
                     /old_locus_tag="I6L24_16450"
     CDS             complement(4657..5607)
                     /gene="repM"
                     /locus_tag="I6L24_RS16445"
                     /old_locus_tag="I6L24_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225810.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005225810.1"
                     /translation="MSRSENLDMSILVSKANDLVKAHYKMTVVEHRLFNIALSKIRSNK
                     ITLDNNVPITISAHEYADLFSDTIDGGYKALRTAVDTLFERQFTLEREISNTKKHVRTY
                     RFIQMKGYLEGEARIEIQFANDVLPFVSELANKFTQIDLQHTVDLSSQYANRLYEILKE
                     VQNYKEQKVFLELDDLRSRFGIENKYKTMSNLKDNVLDLAVTQINKSKACDIYNLQYTN
                     KKAGKKIIGFEFTYKIRKQPKKSDIAIQPIVLKMTDSQRYLFANKLSELTEMNKYSQGT
                     ESYSQFAVRIAEMLQDPLKIEELLPYLKKVGFNTK"
                     /name="repM"
     gene            5911..6090
                     /locus_tag="I6L24_RS16450"
                     /old_locus_tag="I6L24_16455"
     CDS             5911..6090
                     /locus_tag="I6L24_RS16450"
                     /old_locus_tag="I6L24_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005173935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005173935.1"
                     /translation="MKIALGFRYAQTLLNLAFARFVGAIFGSYFREFEKKQKQDQKQLG
                     IRFAHELFFSLRSI"
                     /name="hypothetical protein"
     gene            complement(6220..7656)
                     /gene="mobQ"
                     /locus_tag="I6L24_RS16455"
                     /old_locus_tag="I6L24_16460"
     CDS             complement(6220..7656)
                     /gene="mobQ"
                     /locus_tag="I6L24_RS16455"
                     /old_locus_tag="I6L24_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_216986688.1"
                     /translation="MAIYHFSVKTVARSAGRSATAAIAYRAGEKIYCEREGREHDYSRK
                     TGVEYKEIYLPEGAPEHLNNREKLWNEVEQRETRKNSTVAREFEIAFPSELNQEQRLAM
                     LEELCASIVDRHQVAVDACIHAPHTGSGSDERNYHAHILMSTRKLTPEGFTEKTRELDQ
                     KHSGEIEHWREHFADICNMHLDLAGYTARVDHRSYKDQENGLEATLHEGPKVTELRRKG
                     IETEISRSNDEIKQRNQAQLQYGKNMDLLIAENEIKLSTLKTEQQIQIKNSAKTPPIDE
                     KALFEEKQRETLGKVLKREISAKDANLDLDFMQRNLKQAEINLTKHHKHQNEFNQQLAQ
                     EIVKSGLKQSHDKLQSLVDQHNELTQHKPLLFGKKAWEAQRDAIYQEHKKLKGQHEHQK
                     KHGVKDLLENEKFKEHAWKQYQQQHPAKAKQYQTLYPSYQVIKKCVDEIKAEQQMKLRQ
                     EQQLKAQQQAPKMKSRGMSR"
                     /name="mobQ"
     gene            7866..8135
                     /locus_tag="I6L24_RS16460"
                     /old_locus_tag="I6L24_16465"
     CDS             7866..8135
                     /locus_tag="I6L24_RS16460"
                     /old_locus_tag="I6L24_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_127262076.1"
                     /translation="MSVTETLDSKIKAQEEKLKQLKAQRQAALARERAKEKEQVRKDDT
                     RRKILIGSCMLKITENDEQARAKLIAQMDKYLTDERDRKLFDLI"
                     /name="mobilization protein"
     Typing          complement(6224..7656)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.795"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actggataag cctttaattg taggtacgta cagctaaact taaaaaattg acaaattaaa
       61 aaataaagaa tataaaaaaa cttgtgtgaa ctaaacgcac attttcatat taattaaaag
      121 gataaaacta tgaaattaac gaaagatcaa gtaaaactca tacgagttgg cgaaagtatc
      181 gcaacagttc aattagtctt attttaattt taaataagac taatagataa agatagccac
      241 tattaacata gtggctatct attttaataa taaatagaag agtttgataa tgttttatgg
      301 cttagaaaat ataattgatt ctgaagaatt cattagtgat atttttaata aaaaatcaat
      361 tttaaaaaag aatttttcaa aattacatga agtttttaat ttaattgatt ttgataaata
      421 tttaataaca atggaaggta atttacataa cgttgtaaga attattaaag atcaaaaagg
      481 aatatcaata ccagcgaacg tggataatat tgaaacccaa cgtgattttg tattaagaca
      541 atttagtgaa ggtgctactt taaaacttga agatcttgat tttagaaata ctgaaatagc
      601 taaaatgtgt aaaacattag aaagtattgt tggtggggag gtatttgcta aaccaatatt
      661 aactggaata gggaaaaaaa gttttaatat ccattatgat atgcctgata ttttcgttat
      721 tcagcttgaa ggaacaaaac attggaaagt ttggccaaat catgtaaaaa accctactta
      781 ttctatgcaa gaagtattag tacaagataa tcttcctgat ccggaaatag atatattatt
      841 agaaccagga gatatacttt atattccagg aggaacacca catatagcta gatgtgtaga
      901 tgattattct cttcatatgt caattggtat gattccaatg aaaattcatg atgtattaga
      961 aggttatatt aaaatgctat ctcaacaatt acctatatta agagaaacaa tatatccttt
     1021 ttcatcaaat agtaatttag aagaaattaa aaataaaata atagatcagc ttaattgtat
     1081 accaattaaa aatatggttg atgattatat gttaatgcaa aatgcatata aacatgaaac
     1141 ctcagaatat agacttaaat cttttctttt aagttctaaa ataaacttaa aaagcaaatt
     1201 aaaacttaaa ttaggaagta atctaaatat tagaaagaat agagataaaa ttaatgtata
     1261 ttatagctcg actatagcac ctatagcatt atttaaaata aaccctacat atattgagtt
     1321 tccagaatat tgtgaagatg ctatagatta tttaattaaa atggaaaata ttgaatttca
     1381 ccccgaggac atattaacta aacttgactc tgattccaag atagtccttt gtcaagagtt
     1441 attaaatgct ggaattttaa ttttagtacg tttatagaag taatagaatg tatttaaatt
     1501 tattaagtaa aatattaaga aatacaatat atgaagttaa tgctcctact cctactccat
     1561 tagaaatgtc agagatagaa gagatattag atagtttact aaaagaagga atcgatctta
     1621 atatttctaa agataattta gtggctgcat tacaatatac gaaaaaatca agaaatatac
     1681 atacttatgt tagtgagaaa tcattagata atataaaaag ttgtgttgat ttgattgaaa
     1741 aagattgtgt tccaggtgat cttattgatt gtggggtctt aagaggtggg acatcaattt
     1801 atatggctgg tattttaaaa tcatatggta taaaaaacag aaatcttata gtagctgatt
     1861 cttttgaagg gcttcctcca ccctgtatag aggatggagt tttttctaat gaattttggt
     1921 atagattttc aaaaaactta cctatgtatt ttttagactg ttattgtgat atagaacaag
     1981 taaaaaataa ttttaaatta tatgggctat tatctgaaca ggtaatattt aaaaagggat
     2041 ggttcagtaa aacactagat ccaaaatatt ttaaaaataa tttatctctt ataaggatcg
     2101 atgctgattg gtatcaatca actttagatg tcctagaaag ctgttatcct aagttaagta
     2161 aaggaggtat agtgatcatt gatgattata aactagttgg ttgtagaaaa gcagttgatg
     2221 aattcagaat ttctaacaaa atatttgatg aaattaaatt tgcagataaa gatgccggtg
     2281 taatttggtg gcgtaaataa ttaattagtt tattaatgaa ataaattatg aaaaagaata
     2341 aattattatt gtgttctgaa ggttttagta tatttggtgg gtggattgat tttattgtta
     2401 tattatctat agcagctttt aaattcaata taaattcttt tgaaatttct gttttaagtg
     2461 ctggtatatt attacctagt attgtttttg taaaatatat tggagtactt tctaatagta
     2521 aaaatactat taattatctt atgatttcgc ttttaattag agcaataatt ggaataatta
     2581 taatattttg tgaaagcctg ttaatttttt ccttttttgt tgtactaaga gccacattta
     2641 atagttttaa cttacctgca attcaaacta tagctgctaa taatgtttct gaaactgata
     2701 gaaatagtta ttatactagc ttaaatatag tgaatagtat ttcaaaaatc ttggctccat
     2761 cattaggagg tataatagct cttgtttacg atagtaatta tagtttaatt ttatctagta
     2821 tatttaccgt tattagtttt attataatct tttgtattag aaatagttta aatatagttc
     2881 aaacagaaag aaaaaatgaa aaaaaattaa gcttaaaaac aagtgaatct gaaagaaaaa
     2941 tgtggaattt agttttgcct attatttgca tattttttct ttttgttttt atgataaata
     3001 atcaattacc agtaatttta aaagatttaa aaatgaatga ggcatcttta ggtcttctta
     3061 tatcatccag tgctctagga aatattttat atggattcta caatttaaaa ttaattaaaa
     3121 acacttcgat cgatagttta aagaagttga aaaattctac tttttcaata atgataatat
     3181 ttatattaat cggaattctg attaatagta gtattttatc aataagttac ttgatgattt
     3241 tattttttgt atctggtgtt tttagttcta aattttctat ctatttaaat atttatttat
     3301 ctacttatat gttagaaaat tttggtcaaa taatatcaaa attacaagct atacaaaatt
     3361 taattatttt atttgcccca tttttaggtg cattcattct aattaaatat aatccatcta
     3421 tcctttttat tacatgtgga gttcttggtt tggtgagtat gcttatacta aacattttaa
     3481 ttaaatatag tttatttaaa aatttaaata atgttgaaac ataaaattat attagtggat
     3541 aactagaata agttaatatt caatctaaat tatttagtgt taagctttat tttataggaa
     3601 ttttgatacc tacattttag gtgaagattt atttttttag ctcttcagga agcctagagt
     3661 tactgatttg gctgcgtgta gcattgggct aatgcagcat ttgctttgta tttttacaat
     3721 aagcgtttga tctgacgttg tgtaatatca agtagctgag cagcctgaga ctggattgga
     3781 gaattatttt aattaattta ttttaagatt tcctaaaatt aacataatac accttatacg
     3841 aaatgcttga tatttttctt taaatatcag tatattaaag taagccgttt tggagtatag
     3901 ggcaccagaa cggcttttta ttgattattc atgttccacg gttttattca gtcttaataa
     3961 acctcatccg gttttttggt gagtcattat ttcatgtgct tgcactgaaa ttggctcatc
     4021 aaaaaattgg agtcctggct aaccataagg aagaaaaaaa cggctcagga aacccctttt
     4081 ttttggttca ggctcaacgt gttctggaac agggatacgt ttattgtctt tctgaggagt
     4141 agtcaattcg tcatagtttg actcggtctg tggccgagga tctgttgcta tattcgaatt
     4201 agtagattca tttagtttat taatattttc ttgttttggc tgttctaagg tgcctttaaa
     4261 ctcaattcta tgagtaagat cttcaatatg ttgcatcaaa gacttttctc tttgatttgc
     4321 taaatctaac tgatttttga gtaaagaaat ttgatctttt aatacacttt caacctcaga
     4381 ttgaacgtgt acttgtacat ccttctgaac ttgttcagta cgtgtactgg actgaacacc
     4441 gactggattg ccataaactc tgataagttc agataagtct ataagaccat catgatctct
     4501 tgataattca ccatttttta aagcctcata tattgttgta cgtgattttt taaaggcttt
     4561 acttgcatcc atgacgctga attttgttgt gttcaatgta atccctcagt gcgtgtacaa
     4621 ggatgttcat gtgaacatcc aatctgaaca tgaagtttat tttgtattaa atcctacttt
     4681 ctttagatat ggcagtaatt cttcaatttt aagtggatcc tgcaacattt cggcaatgcg
     4741 aacagcaaat tgagagtaac tttctgtacc ttgagaatat ttattcatct cggtaagctc
     4801 agagagttta ttagcaaaaa gataacgttg cgaatcagtc atttttaaaa ctataggttg
     4861 tatagctata tctgacttct ttggttgctt tctaatttta taggtaaact caaatcctat
     4921 aatttttttc cctgccttct tattagtata ttgaaggtta tatatatcgc aggctttgct
     4981 cttatttatt tgagtgacag ctaagtccag gacattgtct tttagatttg acatagtttt
     5041 atatttattt tcgataccga acctagatcg aagatcgtcc aactctagaa aaactttttg
     5101 ctctttatag ttctgcactt ctttaagaat ttcatataag cgattggcat attggctgct
     5161 taaatcaacc gtatgttgaa gatcaatttg agtaaattta ttagctagct cacttacaaa
     5221 tggtaagacg tcattagcaa attgaatttc aattctagct tcaccttcaa gatatccctt
     5281 catttgaata aatctatatg ttctaacgtg ttttttagta tttgagattt ctcgctctaa
     5341 tgtgaactgg cgttcaaata aagtatctac agccgttcta agggctttat aaccgccatc
     5401 tatggtgtct gaaaacagat ctgcatattc atgagctgaa atggtaattg gtacattatt
     5461 atctaatgta atcttattag aacgaatttt tgataaagcg atattgaaca atctgtgctc
     5521 aacaacagtc attttataat gagcttttac taaatcattt gctttagaga caagaatcga
     5581 catgtctaaa ttttctgaac ggctcattct tatttcccta taaacattat atggatttac
     5641 caaaatctag ataaattttt cacttaggga atcatcataa caatactcat ttaaatagta
     5701 aaataaaaac ctatgtttat acggataaaa cctatgtttg aaacggataa aacctatgtt
     5761 tgaaacggat aaaacctatg tttatacgga taaaacctat gtttgaaacg gataaaacct
     5821 atgtttatac caccataata tattgaaaac aaaaaagaaa aaatgcttca aatattaaat
     5881 attataaata taaaataaaa aagaaaattc atgaaaattg cccttggttt tcgctacgct
     5941 caaactctat tgaatctcgc tttcgctcga ttcgttgggg caatttttgg ttcatatttt
     6001 cgtgaatttg agaaaaagca aaagcaagat caaaagcagc tcggaattcg cttcgctcat
     6061 gagctttttt tctcgctacg ctcgatttag gaatcaaact tggaaagttc gcacccatgc
     6121 gggatgctct gtgatgcagg tgattgatgg atagggagct tgtaatcagg gaaagggatg
     6181 ggcaaatttt ctcagttcgc tcgtagacac tcgctctgtt tatcgactca tgccacgaga
     6241 tttcatttta ggagcttgtt gctgtgcttt gagctgttgt tcttgtctga gtttcatctg
     6301 ttgttctgct ttgatctcat ccacacattt tttaatgact tgataggatg ggtatagagt
     6361 ctggtattgc ttggctttgg ccggatgttg ttgttggtac tgcttccagg catgttcttt
     6421 aaacttttca ttttccaata aatccttcac accatgtttt ttctggtgtt catgctgacc
     6481 tttcagcttt ttgtgttcct ggtaaatcgc atcacgttgg gcttcccaag ctttttttcc
     6541 aaacagtaag ggtttgtgct gggttaattc gttgtgttga tcgaccaggc tttgcaattt
     6601 gtcatgactt tgcttgagtc cgcttttcac gatctcctgg gcaagctgct gattgaactc
     6661 gttttgatgt ttgtggtgct tggtcaggtt tatttcggct tgtttgagat tgcgctgcat
     6721 aaaatccagg tctagattcg cgtcttttgc gctgatttcg cgttttagca ctttgccaag
     6781 ggtttccctc tgtttttcct caaacagggc tttttcgtca attggtggcg ttttagcgct
     6841 atttttgatt tggatctgct gttcagtttt cagtgtgctg agtttgatct cattttcagc
     6901 aatcagcaga tccatatttt tgccgtattg cagttgagcc tgatttctct gtttgatttc
     6961 atcattgctg cggctgattt cggtctcaat cccttttcgg cgtagttcgg tgactttcgg
     7021 tccttcgtgc agggtggctt ccaggccatt ctcctggtct ttgtagctgc gatggtcgac
     7081 tcgggcggtg tatccggcta gatccaggtg catattgcag atatcggcaa agtgttcgcg
     7141 ccagtgttca atttctccac tgtgtttctg atccagttcc cgggttttct cggtaaaacc
     7201 ttccggagta agtttgcgtg tgctcatcag gatatgggcg tgataattgc gttcatcact
     7261 gccggatccg gtatgcggtg catggataca ggcatcgacg gccacctgat gtctgtccac
     7321 gatactggcg catagttctt cgagcatggc cagacgttgt tcctggttca actcacttgg
     7381 aaaggcaatt tcaaattccc gggcaactgt cgagtttttt cgggtttcgc gttgttcgac
     7441 ttcgttccag agtttttctc gattatttaa atgttctggt gcgccttcgg gtaagtaaat
     7501 ctctttgtat tccaccccgg tttttcggct gtagtcatgt tctcggcctt cacgttcgca
     7561 ataaatcttt tcaccggccc gataggcaat cgcggcagtg gcggatcgcc ctgctgatcg
     7621 tgctacggtt ttgactgaaa agtggtaaat cgccatgtct atttttcgct tttaaatttt
     7681 cacgtagtga aatacaaccg tggatacggt tgtcggggta ttggggagac cccaatcgta
     7741 cttgcaaacg aagtttgcat aagtgcgctc ttcgaggaaa acattactat acatatagca
     7801 ccttgaatgc atattcaagt gcgcttatgt tagggatggt ttcccaggat gatggtatcg
     7861 gattaatgag cgttactgaa acactagaca gcaaaattaa agcccaggaa gaaaagctga
     7921 aacagctcaa agctcaaaga caggcagcac tggcaagaga acgtgccaaa gagaaagaac
     7981 aggtacgaaa ggatgatacc agacgcaaga ttttaattgg atcctgcatg ttgaaaatta
     8041 ccgaaaatga tgaacaagct agagcaaaac tgattgctca gatggataag tatttaacgg
     8101 atgaaagaga tcgtaagctg tttgatttaa tctgaattac ttaagataaa tatctgtagt
     8161 ttataaaatc tgaatttttg gcttatttcg atggtttaat tgatatgcac acaatccatt
     8221 catagatccc acttaaaaag tcagtgatac tacacacttt
//
