LOCUS       NZ_CP077374             8723 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter lwoffii strain FDAARGOS 1394 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP077374
VERSION     NZ_CP077374.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357536
            Assembly: GCF_019048525.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8723)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.1.1, SPAdes v. 3.14.1
            Genome Coverage                   :: 8261.12x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:39:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,330
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 111
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 38 of 119
            Pseudo Genes (incomplete)         :: 73 of 119
            Pseudo Genes (internal stop)      :: 32 of 119
            Pseudo Genes (multiple problems)  :: 20 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077374.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1394"
                     /culture_collection="DSM:101862"
                     /db_xref="taxon:28090"
                     /plasmid="unnamed5"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            complement(join(8573..8723,1..374))
                     /locus_tag="I6L24_RS16360"
                     /old_locus_tag="I6L24_16365"
     CDS             complement(join(8573..8723,1..374))
                     /locus_tag="I6L24_RS16360"
                     /old_locus_tag="I6L24_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008307689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_127262066.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPSKKLDVKESVKIDSPNSAEVLLLRQQIDMLKNQLDDAKERESFYQNQIEMMQRLL
                     EAPKPKIQEPSEPELPKSAPIVEMKEEVIVVPPKENRLTTPENKRIPVPEHVEPEPKKR
                     GFLSRFFLPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(453..1382)
                     /gene="repM"
                     /locus_tag="I6L24_RS16365"
                     /old_locus_tag="I6L24_16370"
     CDS             complement(453..1382)
                     /gene="repM"
                     /locus_tag="I6L24_RS16365"
                     /old_locus_tag="I6L24_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_127262067.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLRDASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YEPQSGIVFLRFTQDIVPLITRLEENFTKYELEQVSRLTSSYAIRLYELLIQWRSAGKT
                     PIFDLSIFRQQLGVEAHQYKTMSNFKTYVLEFALKQVNELTDITAKYEQHKKGRSISGF
                     SFSFKQKKTNSDKVIKGTDTLALFSKMSDKQRHLFANKLSELPEMGRYSEGTESYQQFA
                     VRIAEMLQDSQKFQELFPYLQKVGYQAA"
                     /name="repM"
     gene            complement(1957..3393)
                     /gene="mobQ"
                     /locus_tag="I6L24_RS16370"
                     /old_locus_tag="I6L24_16375"
     CDS             complement(1957..3393)
                     /gene="mobQ"
                     /locus_tag="I6L24_RS16370"
                     /old_locus_tag="I6L24_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_216986685.1"
                     /translation="MAIYHFSVKTVARSAGRSATAAIAYRAGEKIYCKREGREHDYSRK
                     TGVEYKEIYLPEGAPEHLKNREKLWNEVEQRETRKNSTVAREFEIAFPSELNQEQRLAM
                     LEELCASIVERHQVAVDACIHAPHTGSGSDERNYHAHILMSTRKLTPEGFTEKTRELDQ
                     KHSGEIDHWREHFADICNVHLNLAGSTARVDHRSYKDQENGLEATLHEGPKVTELRRRG
                     IETEISRSNDEIKQRNQAQLQYDKNMDVLIAENEIKLSTLKTEQQIQIKNSAKTPPIDE
                     KALFEEKQRETLGKVLNREISAKDANLDLDFMQRNLKQAEINLTKHHKHQNEFNQQLAQ
                     EIVKSGLKQSHDKLQSLVDQHNELTQNKPLLFGKKAWEAQRDEIYQEHKKLKGQHEHQK
                     KHGVKDLLENEKFKEHAWKQYQQQHPAKAKQYQTLYPSYQVIKKCVDEIKAEQQMKLRQ
                     EQQLKAQQHAPKMKSRGMSR"
                     /name="mobQ"
     gene            3603..3869
                     /locus_tag="I6L24_RS16375"
                     /old_locus_tag="I6L24_16380"
     CDS             3603..3869
                     /locus_tag="I6L24_RS16375"
                     /old_locus_tag="I6L24_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_216986687.1"
                     /translation="MSVTETLDSKIKAQEEKLKQLKAQRQAALARERAKEKEQARKDDT
                     RRKSLIGSCMLKITEDDEQARAKLIAQMDKYLIDERDRKLFNL"
                     /name="mobilization protein"
     gene            3887..4318
                     /locus_tag="I6L24_RS16380"
                     /old_locus_tag="I6L24_16385"
     CDS             3887..4318
                     /locus_tag="I6L24_RS16380"
                     /old_locus_tag="I6L24_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_216986686.1"
                     /translation="MEDPTLDELIQKTKLFFEHHWNADLLGEPPEWSSVHFNFGKIPNY
                     DKQGVYAFIKGDLVTYIGVGTSRGAGRYRGNGLSARVMKYCRWNEDKTEYIAINPRLKD
                     AGSIITIGFERDRAYLANALEVFLISKLHPVHNVVKVGM"
                     /name="hypothetical protein"
     gene            complement(4404..5225)
                     /locus_tag="I6L24_RS16385"
                     /old_locus_tag="I6L24_16390"
     CDS             complement(4404..5225)
                     /locus_tag="I6L24_RS16385"
                     /old_locus_tag="I6L24_16390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_216986681.1"
                     /translation="MKNPENINIDDFESIKFFGVMFIKNLQKIANNQIKLDKEIPESIK
                     IDWRIISEWLKLEHSLQELDTNHLEIVEKAWLAYLARGDAPSLKLKPVFQKFSSIPKKN
                     EYLKNLPPPIIINIFDSLLATREEIVKKRLEDNLSQMKDKNILSTIHGCFSILWIPIVL
                     FFLLGFFSILSDIYSLKPIIYIFIFIILSFIFWKKIDFIKIKYLIHTYRKKLFYSSIWV
                     LLSSFIIYVFNPLELDWNYLEFIDYLKAGFIITLPILYLCIEIIYKKFIDI"
                     /name="hypothetical protein"
     gene            complement(5298..5612)
                     /locus_tag="I6L24_RS16390"
                     /old_locus_tag="I6L24_16395"
     CDS             complement(5298..5612)
                     /locus_tag="I6L24_RS16390"
                     /old_locus_tag="I6L24_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_216986682.1"
                     /translation="MSTVRYSRKELNEKFSEKQDAEIERLLTKGTVPDDQLDLSDIPEI
                     TDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(5599..5886)
                     /locus_tag="I6L24_RS16395"
                     /old_locus_tag="I6L24_16400"
     CDS             complement(5599..5886)
                     /locus_tag="I6L24_RS16395"
                     /old_locus_tag="I6L24_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_120366266.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFDDPLRISIQDRHTDG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERDKYEHG"
                     /name="BrnT family toxin"
     gene            6197..6583
                     /locus_tag="I6L24_RS16400"
                     /old_locus_tag="I6L24_16405"
     CDS             6197..6583
                     /locus_tag="I6L24_RS16400"
                     /old_locus_tag="I6L24_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017030686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_216986683.1"
                     /translation="MLKKTFYGLSILTLISITAHAETTYRTDSFGNTRATDSYGNTTTY
                     RTDSFGNTRGSDGSNMRTDSFGNTRATDSYGNTTTYRTDSFGNTRGNDGSNMRTDSFGN
                     TRITDSNGNTQTCRKDSFGTTRCN"
                     /name="hypothetical protein"
     gene            complement(6675..6956)
                     /locus_tag="I6L24_RS16405"
                     /old_locus_tag="I6L24_16410"
     CDS             complement(6675..6956)
                     /locus_tag="I6L24_RS16405"
                     /old_locus_tag="I6L24_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985610.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="WP_000985610.1"
                     /translation="MAIKLRKWDSAEHLKTEEDMQAYLQACIDESNGDAAFIAKALGNI
                     AKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTVIKVVKALNLRLAI"
                     /name="addiction module antidote protein"
     gene            complement(6957..7259)
                     /locus_tag="I6L24_RS16410"
                     /old_locus_tag="I6L24_16415"
     CDS             complement(6957..7259)
                     /locus_tag="I6L24_RS16410"
                     /old_locus_tag="I6L24_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286964.1"
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000286964.1"
                     /translation="MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVG
                     EGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKSTQSKDIKLALQLAQDLEEEL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7432..8226
                     /locus_tag="I6L24_RS16415"
                     /old_locus_tag="I6L24_16420"
     CDS             7432..8226
                     /locus_tag="I6L24_RS16415"
                     /old_locus_tag="I6L24_16420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_216986684.1"
                     /translation="MQSLFSKIFLLNIFIMVGGVAHAIEVKPFKSIKNDDGYPEDIDLY
                     KITNKYSYLYIKQYPFYIENSNSNAGFISEIDPQCEILEKIYKQQQTTYIVTSTCGSWF
                     GMSSAESHYVIYEFNDVSKLIKSETIKFKESLDITISELNSGIKIASSPSPKTGKKYNY
                     EYKNGSLYDFSTSMNRNLVSKENDRLCKGFYNVFIRYEYEYKPDVKVNSIYDIGTANYS
                     WASHKIKSTPRLEEKFLSLLKAKTPKQRKQISYENFKKEYCD"
                     /name="hypothetical protein"
     Typing          complement(455..1382)
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.489"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1961..3393)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.795"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgggagctg atttagggag ttcgggttca ctaggctctt ggatctttgg tttgggtgct
       61 tccagtagac gttgcatcat ctcaatttga ttttgataaa aggattcacg ctcttttgca
      121 tcatcaagtt gatttttcag catgtctatc tgttgcctaa gtagtaaaac ttctgccgaa
      181 tttggactgt ctatttttac agactctttt acatctagtt tttttgaagg ttcaccaaaa
      241 acacggatag cctcggctag atcaatttta ttatcagaat ttttacttaa aattcctttc
      301 tttatgttgt tgtaaatcgt ttgtctattg atattgtaaa gcttagaaag ctcaagaact
      361 gtaaggcttt tcatgttgta aatctatgcc taactctgtc aaaagttgtt gtctattaga
      421 ctgcctaata gacaaatgat caagtataaa ttttaggcag cttgatagcc aactttttga
      481 agataaggga ataattcttg gaatttttga gaatcttgaa gcatttctgc aatgcgcaca
      541 gcaaattgct ggtagctttc ggtgccttct gaatatcgcc ccatctcagg taattcagaa
      601 agtttgttgg caaagagatg acgttgttta tcactcattt tggagaaaag agctaatgta
      661 tccgttccct taattacttt gtcagagttg gtttttttct gcttaaaact gaaagaaaaa
      721 ccggaaattg aacgtccttt tttatgttgt tcatatttag ccgttatatc ggttaactca
      781 tttacttgct tgagggcaaa ctctaaaaca tacgttttaa aattactcat tgttttgtat
      841 tgatgagctt cgacaccaag ttgttgtcta aagatcgata gatcaaaaat tggcgtttta
      901 ccggcagatc tccattgaat taataactcg tataaccgaa tagcataaga gctagttaat
      961 ctcgaaacct gctccaattc atatttagta aagttttctt ccagtcgagt tatgagtgga
     1021 acaatgtctt gcgtgaaacg taggaaaacg atcccggatt gaggttcata tccaatttta
     1081 tcgacccaac gacagtgaaa gctacgatcc ttaccagtct taggattaat gtcatgatac
     1141 gtgacatagc gatcaaataa gcttttagat gcatcccgaa gcacggtata agcagtgtgt
     1201 ttctcgacat taaaggtatt gatataacta cttgcatgta cttctaaaag gctattttca
     1261 gttatcccat gccctgtttc tcgagcttca gcaatggcta ataggatcag cctttgttca
     1321 actgtatcta gggtatagct agcttgaatt aaagcattat cctttacgat taattcactc
     1381 atttctaata ataaatttat aattaaacta ggtcgtacaa tagactattt taattgattt
     1441 gtaaatcatc ttgatttgag gtcgataaac ctagttttaa ggtcgataaa cctagtttta
     1501 tgggtcgata aacctagttt tatgggtcga taaacctagt tttatgggtc gataaaccta
     1561 gttttattgg ctctgaaacc cttgtgtata aaggattaca gcttacttaa aagcatttaa
     1621 aagattaaaa ataattaaaa gcctaaagta tgaaaaattg cccttggttt tcgcttcgct
     1681 caaactctat tgaatctcgc tttcgctcga ttcgtcgggg caatttttgg ttcatatttt
     1741 cgtgactttt agaaaagcaa aagcaagatc aaaagcagct cggaattcgc ttcgctcatg
     1801 agcttttttc tcgctacgct cggtttggga taaaacttgg gaagttcgca cccattcggg
     1861 atgctctgta gggtaggtga ttaatggata gggagcttgt aatcaaggaa aggaaagggc
     1921 aaattttctc agttcgctcg tagacactcg ctctgtttag cgactcatgc cacgagattt
     1981 cattttaggc gcatgttgct gtgctttgag ctgttgttcc tgtctgagtt tcatctgttg
     2041 ttctgctttg atctcatcca cacatttttt aatgacttga taggatgggt atagagtctg
     2101 gtattgcttg gctttggctg gatgttgttg ttggtactgc ttccaggcat gttctttaaa
     2161 cttttcattc tccaataaat ccttcacacc atgttttttc tggtgttcat gctgaccttt
     2221 cagctttttg tgttcctggt agatctcatc acgttgggct tcccaggctt tttttccaaa
     2281 cagtaagggt ttgttctggg ttaattcgtt gtgttgatcg accaggcttt gcaatttgtc
     2341 atgactttgc ttgagtccgc ttttcacgat ctcctgggca agctgctgat tgaactcgtt
     2401 ttgatgtttg tggtgcttgg tcaggtttat ttcggcttgt ttgagattgc gctgcataaa
     2461 atccaggtct agattcgcgt cttttgcgct gatttcgcga tttagcactt taccaagggt
     2521 ttctctctgt ttttcctcaa acagggcttt ttcgtcaatt ggtggcgttt tagcgctatt
     2581 cttgatttgg atctgctgtt ctgttttcag tgtgctgagt tttatctcat tttcagcaat
     2641 tagaacgtcc atatttttgt cgtattgcaa ctgagcctga tttctctgtt tgatttcatc
     2701 attgcttcgg ctgatttcgg tttcgatgcc tcttctacgt agttcggtga ctttcggtcc
     2761 ttcgtgcagg gtggcttcca ggccattctc ctggtctttg tagctgcggt ggtcgacccg
     2821 ggctgtggat ccggccagat tcaggtgcac attgcagata tcggcaaagt gttcacgcca
     2881 gtgatcgatt tctccactgt gtttctgatc gagttcccgg gttttctctg taaaaccttc
     2941 tggcgtaagt ttacgtgtgc tcattaggat atgggcgtga taattgcgct catcactgcc
     3001 ggatccggta tgcggtgcat gaatacaggc atcgactgct acctgatgtc tttccacgat
     3061 gctggcacat aattcttcga gcatggccag acgttgttcc tggttcagtt cacttggaaa
     3121 ggcaatttca aattccctgg ctacagtcga gttttttcgg gtttcccgtt gttcaacttc
     3181 gttccagagt ttttctcgat tctttaaatg ctctggtgcg ccttctggta aataaatttc
     3241 tttgtattcc acaccggttt ttcggctgta gtcatgttct cggccttcac gtttgcaata
     3301 gatcttttca ccggcccgat aagcaatcgc agcagtggcg gaacgccctg ctgatcgtgc
     3361 tacggttttg actgaaaagt ggtaaatcgc catgcctgtt tttcgctttt aaattttcac
     3421 gtagtgaaat acaaccgtga atacggttgt cggggtattg gggagacccc aatcgtactt
     3481 gcaaacgaag tttgcataag tgcgctcttc gaggaaaaca ttactataca tatagcacct
     3541 tgaatgcata ttcaagtgcg cttatgttag gaatggtttc ccaggatgat ggtattggat
     3601 taatgagtgt tactgaaact ttagacagca agattaaggc acaggaagag aagctaaaac
     3661 agcttaaggc tcaaagacag gcagcattag caagagaacg tgccaaagag aaagagcagg
     3721 cacgaaagga tgataccaga cgtaaaagtt taatcggatc ctgcatgttg aaaatcactg
     3781 aagatgatga acaagccaga gcaaaattga ttgcacagat ggataagtat ttaatagatg
     3841 agcgtgatcg taagcttttt aatttataaa ttttgagaga tgagttgtgg aagatccaac
     3901 acttgatgag ttaatacaga aaacaaaact attctttgaa catcactgga atgcagatct
     3961 attaggagag cctcctgaat ggtcatcagt acattttaat tttgggaaaa ttcctaatta
     4021 cgataaacaa ggtgtatatg cttttatcaa aggggattta gtgacttata ttggtgttgg
     4081 gacttctaga ggtgctggtc gttatcgtgg taatggctta agcgccaggg taatgaaata
     4141 ttgccgatgg aatgaagata aaactgaata tattgcgata aatccaagac tgaaagatgc
     4201 cggatccatc attacgatag gctttgaacg tgatagggct tatttagcaa atgctctaga
     4261 ggtttttctt atctctaaac tccatcctgt ccacaatgtg gttaaagttg gtatgtaatt
     4321 tcgcataaga gattttatgt taaataaaaa tctatcttat agatgctatt aataaattga
     4381 agttttactt atccagtaat taatcatata tctatgaatt ttttataaat tatttcaatg
     4441 cataaatata atattggcaa tgttataata aatccagctt ttaaataatc tataaattct
     4501 agatagttcc aatccaattc taacgggttg aaaacgtaaa taataaaact agacaataaa
     4561 acccaaatag atgaataaaa aagctttttt ctatacgtat gaattaaata ttttattttg
     4621 atgaaatcta tttttttcca aaaaataaat gaaagtataa tgaagataaa tatatatatg
     4681 attggtttta aactatatat atctgataat attgaaaaaa aacccaataa aaaaaataaa
     4741 actataggta tccaaagaat tgagaagcaa ccatgtattg ttgataagat gtttttatct
     4801 ttcatttgtg aaagattatc ttctaacctc tttttaacta tttcttctct ggtagcgagt
     4861 aagctatcga aaatattaat tatgattggt ggaggtaggt tttttaggta ctcatttttc
     4921 ttaggaatag atgaaaattt ttgaaatact ggttttaact ttaatgaagg agcatctccc
     4981 ctagccaagt aggctaacca tgctttctcc acgatttcta aatgatttgt atcaagttct
     5041 tgaagtgagt gttctaattt cagccattct gaaataattc tccaatcgat ttttatagat
     5101 tcaggtattt ctttatctaa ctttatttgg ttattagcga ttttttggag attttttata
     5161 aacattacac caaaaaattt gatggattca aaatcgtcaa tattgatatt ttcaggattt
     5221 ttcataagat ttctttaaat taacataatg ggcattatgc gaagttcaca caaaaatggg
     5281 agtataaact cccattttta ttgatgattt tttaattctt tcagcatagc atctcgcaga
     5341 atctcattca tccgggtttg atagccttta ccttgagctt taaaccatgc aagtacatca
     5401 gcatctaaac gaatggaggt ctgttgcttc actgggcgat agaattgatt atggcgtaca
     5461 gcattgctcc agtctgtaat ttcaggaata tctgataaat ctagctgatc atcaggaacc
     5521 gtccccttcg taagcaagcg ttcgatttca gcatcttgct tctcactaaa tttttcattt
     5581 agttctttgc gtgagtatct aaccgtgctc atatttatcg cgctccgcct tagtgacttg
     5641 tcttgcacta atgattcgta tgatttcaca gtcatcttca tcaaagatgg tgtgagctac
     5701 taatagcatt agtacgcctt ttactcttcc aatggtttgc caacgttctt caccatcagt
     5761 atgtctgtcc tggattgata tccttaatgg atcatcaaag acaaggcttg cagtttcgaa
     5821 agagatatca tgctttttct gattctttcg attctttgcc tcatcccatt cgaaatattg
     5881 ttccataaaa aacattcaaa ttcgtatata caataatgta tcacaaaatt gtatcactca
     5941 agttgaaaag cgtaattctt agtcaaattt attggcaaaa catatcaaaa ccgctcataa
     6001 aaaagccgac ttgtttcaaa atcggctttt tggtcatttt agaatttttc tgatttgaat
     6061 aagtaactga tattccaatt aatatacaag tgtacttcgt ataaccgcca ttatgttaaa
     6121 tggaggagag gaactttttt aaatcgtcct atatacttta ctaaaattac ttataaacaa
     6181 tatgcggaat tttattatgt taaaaaaaac tttctatggc ttaagtatct taaccctcat
     6241 ttctataaca gctcatgctg aaacaactta tcgtacggat agttttggta atacacgtgc
     6301 tactgattct tatggtaata cgacgactta tcgcacagat agctttggta atacgcgtgg
     6361 tagtgatggt tctaatatgc gtacagacag ctttggtaat acacgtgcta ctgattctta
     6421 tggtaatacg acgacttatc gtacagatag ctttggtaat acgcgtggta atgatggttc
     6481 taatatgcgt acagacagct ttggcaatac gcgtattaca gactctaatg gtaatacaca
     6541 aacgtgcaga aaggattcct ttggaaccac tcgatgtaac taattcaaaa tttaattttc
     6601 tctaaaatta acatactaca cgttatatta agttgaaaaa gggagcttta agctcccttt
     6661 ttgactagtt ttttttaaat tgctaagcgt aaattcaatg cttttacgac tttaataaca
     6721 gtatcaaagc ttggatttac atcgccagaa agtgctttat aaagactttc tcggcctaaa
     6781 ccagtgtctc ttgataattg agccattccc ttagcttttg caatattacc taaagctttt
     6841 gcaatgaatg ccgcatcacc attagattca tcgatacagg cttgtaggta agcttgcata
     6901 tcttcttctg ttttaaggtg ttcagcactg tcccatttac gaagtttgat agccatttac
     6961 agctcctcct ctaaatcttg tgctaattgt agagcaagtt ttatatcttt actttgcgta
     7021 gacttgtctc cgcctgctaa aagaataaca accctttgcc cttgcttaca gtaatagatc
     7081 ctgtagccag gtccaaagaa gaaacgtaat tcagagacac cttcaccgac tggttcagta
     7141 tctccaaagt ttccatcttc aactcgatca attcggactt gtatgcgtct ttttgcctgc
     7201 tggtctttta acttggtaaa ccagtcatca aagacttcag tggtatatat tgagtacata
     7261 agaataatgt atcctaaggg atacacttat gcaagagata aacttgaata tatggggcta
     7321 caaaaaaatc gtatgacaac cattatctta aatggctgta tggatatttt accttttttt
     7381 caaactagta gttaaaatca tttcaaaatc taaacttata aggtatttaa agtgcaaagc
     7441 cttttttcta aaatatttct actaaatatc ttcataatgg taggtggcgt tgcgcacgct
     7501 atcgaagtca aaccattcaa atctataaaa aatgatgatg gttatccaga agatattgat
     7561 ctatataaaa taacaaataa atacagttat ttatatataa aacaataccc attttatatt
     7621 gagaacagta atagtaatgc tggttttatt agtgagatag atccacaatg tgaaatctta
     7681 gaaaagatat ataaacaaca gcagactact tatattgtaa caagtacatg tggctcatgg
     7741 tttggcatga gtagtgcaga aagccattat gttatatatg agtttaacga tgtatcaaaa
     7801 ttaattaaat ctgaaactat taaatttaaa gagtcattag atattacgat ctcggagctg
     7861 aattcaggta taaaaatagc atcaagtcca agtccaaaaa ctgggaaaaa atataattat
     7921 gaatataaaa atgggagctt atatgacttc tcaacatcta tgaatagaaa tttagtttca
     7981 aaagagaatg atagactatg taagggattc tataatgtat tcataaggta cgaatatgaa
     8041 tacaagccag atgtaaaagt aaacagtatc tatgatatag gtactgctaa ttacagttgg
     8101 gcatcacata aaattaaatc tacaccacga ttagaagaaa agttcctcag cttgctaaaa
     8161 gcaaaaactc caaaacaaag aaaacaaata tcttacgaga atttcaaaaa agaatattgt
     8221 gattagatta agaatcaaag attagaatct taaagattca ctaatagaat tttaaaaacc
     8281 ttaagcctaa aagaattcgc aaataagact aatgaggttt tttaaaatta acataataca
     8341 cgttatacga aatgctcttt tttattctat ataaatcata gtcttatcgt atacaaaaaa
     8401 gcccaactgc actcagttgg gcttttttaa tgatttttca tgttccacgc ttactattta
     8461 agcaatgttc cacggttttg ttcagtttta ataaacctca tccggttttt tgatgagtca
     8521 ttatttcatg tgcttgcact gaaattggct catcaaaaaa ctggagtcct ggctaaccat
     8581 aaggaaggaa aaaacggctc aggaaacccc tttttttagg ttcaggctca acgtgttctg
     8641 gaacagggat acgtttgttc tctggagtag ttaatctgtt ctcttttggt ggaactacta
     8701 tcacctcttc cttcatttct aca
//
