LOCUS       NZ_CP077373            19982 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter lwoffii strain FDAARGOS 1394 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP077373
VERSION     NZ_CP077373.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357536
            Assembly: GCF_019048525.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 19982)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19982)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.1.1, SPAdes v. 3.14.1
            Genome Coverage                   :: 8261.12x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:39:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,330
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 111
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 38 of 119
            Pseudo Genes (incomplete)         :: 73 of 119
            Pseudo Genes (internal stop)      :: 32 of 119
            Pseudo Genes (multiple problems)  :: 20 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077373.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..19982
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1394"
                     /culture_collection="DSM:101862"
                     /db_xref="taxon:28090"
                     /plasmid="unnamed4"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            join(19828..19982,1..769)
                     /gene="repM"
                     /locus_tag="I6L24_RS16240"
                     /old_locus_tag="I6L24_16245"
     CDS             join(19828..19982,1..769)
                     /gene="repM"
                     /locus_tag="I6L24_RS16240"
                     /old_locus_tag="I6L24_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282204.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_004282204.1"
                     /translation="MKTELIVKDNALINASYNLDLVEQRLILLAIVEARESGKGINAND
                     PLTVHAESYINQFGVHRNTAYQALKDACDDLFARQFSYQSLSEKGNVINHKSRWVSEVA
                     YIDNEAVVRLIFAPAIVPLITRLEEQFTKYEIQQISNLTSAYAVRLYEILIAWRSTGKT
                     PLITIYDFRQKIGVLETEYKRMYDFKKYVLDIALKQVNEHTDINVKVEQHKTGRSITGF
                     SFSFKQKKSATNTANDISQDKELKINLTDAQRYLFASKLSELPEMAKLSQGNESYEQFA
                     ARIVTMLQEPNKLKEFMPLLRKVGFQ"
                     /name="repM"
     gene            837..1373
                     /locus_tag="I6L24_RS16245"
                     /old_locus_tag="I6L24_16250"
     CDS             837..1373
                     /locus_tag="I6L24_RS16245"
                     /old_locus_tag="I6L24_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_216986675.1"
                     /translation="MKKLSVSELAKLYGYSRQAVYAHIKKGNLSKGSDGLIDFSEALRV
                     FGEPQKSNSSVNQSQSTSRHNLTEVDLLKRQVDMLEKQLNQAIQRENQSLERESFYQEQ
                     IEAMQRLLEAPKANMTTFTDHGSEQDIATDSRPQTESNYDGLTTPENKRIPVPEHIEPT
                     PKKRGFLSRFFLPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(1656..3110)
                     /locus_tag="I6L24_RS16250"
                     /old_locus_tag="I6L24_16255"
     CDS             complement(1656..3110)
                     /locus_tag="I6L24_RS16250"
                     /old_locus_tag="I6L24_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995702.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_131275007.1"
                     /translation="MLSNVREQWFSNIRGDVLAGLVVGLALIPEAIAFSIIAGVDPKVG
                     LYASFCIAVVISFVGGRPAMISAATGAMALLMVTLVKEHGLEYLLAATILTGIIQILAG
                     YLKLAKLMRFVSKSVVIGFVNALAILIFMAQLPELINVTWHVYALVALGLAIIYLFPLV
                     PKLGKLLPSPLVCIIAITLLAIALGIDVRTVGDMGSLPDTLPMFLIPNIPLNIETLMII
                     LPYSVALAAVGLLESMMTATIVDEMTDTPSDKYQECKGQGIANIASGFMGGMAGCAMIG
                     QSMINVKSGGLTRLSTFSAGIFLLILVVFISDWLKVIPMAALVAVMIMVSISTFEWSSV
                     TQLKDNPKSSNIVMIATVIVVVATHNLALGVLTGVLLSALFLANKLENDIQVTASFEGT
                     ARLYDLRGQIFFSSSEKFMQGFDFKEDVKDVIIDLTHSHIWDVTSVAMLDSVVNKFQKN
                     GVNVTVRGLNEASSIMIDKYGTHARI"
                     /name="SulP family inorganic anion transporter"
     gene            complement(3358..3567)
                     /locus_tag="I6L24_RS16255"
                     /old_locus_tag="I6L24_16260"
     CDS             complement(3358..3567)
                     /locus_tag="I6L24_RS16255"
                     /old_locus_tag="I6L24_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010116191.1"
                     /translation="MFNFKIFNKVSAEVLTIKNDLQLNAELQLINKYKTATSEDYKQAI
                     VLIFKERGYTRLEIGQLLGELKAS"
                     /name="hypothetical protein"
     regulatory      3766..3833
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
                     /name=""
     gene            3950..4330
                     /gene="crcB"
                     /locus_tag="I6L24_RS16260"
                     /old_locus_tag="I6L24_16265"
     CDS             3950..4330
                     /gene="crcB"
                     /locus_tag="I6L24_RS16260"
                     /old_locus_tag="I6L24_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225409.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="WP_005225409.1"
                     /translation="MYLSLLSIALGSVIGAWLRWGISLRFNSVFENIPFGTVIVNLIGA
                     FIIGLAVSFFSNSSISPNYKLFVVTGFCGALTTFSTFSVEIVALLQASKFEYAISTIAI
                     HVIGSLIFTVLGILSYQFFTNH"
                     /name="crcB"
     gene            4357..4881
                     /gene="ppa"
                     /locus_tag="I6L24_RS16265"
                     /old_locus_tag="I6L24_16270"
     CDS             4357..4881
                     /gene="ppa"
                     /locus_tag="I6L24_RS16265"
                     /old_locus_tag="I6L24_16270"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225406.1"
                     /GO_function="GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /GO_process="GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="WP_131275023.1"
                     /translation="MTFKNVKAGTNPPDDFNSIIEIPANAKPIKYEVNKEYDALLVDRF
                     LSTAMSYPYNYGYIPQTLSEDGDPLDVFVIAPHAVAVGSVIRSRPVGVMYMEDEAGIDA
                     KIIAVPHSKLTPLYNNVEDLSDLPQLQLDQMKHFFEHYKDLEKGKWMKFNGWGNVEEAR
                     QEILKSIQAYK"
                     /name="ppa"
     gene            complement(5388..5660)
                     /gene="nadS"
                     /locus_tag="I6L24_RS16270"
                     /old_locus_tag="I6L24_16275"
     CDS             complement(5388..5660)
                     /gene="nadS"
                     /locus_tag="I6L24_RS16270"
                     /old_locus_tag="I6L24_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_216986676.1"
                     /translation="MDNSLFDDLVASIKEAGAIKRNEIKASRVTELELPDIKEVREKTG
                     LTQAEFAARLHISARTLQNWEQGRRYPTGPAATLIRILDAHPTLI"
                     /name="nadS"
     gene            complement(5653..5973)
                     /locus_tag="I6L24_RS16275"
                     /old_locus_tag="I6L24_16280"
     CDS             complement(5653..5973)
                     /locus_tag="I6L24_RS16275"
                     /old_locus_tag="I6L24_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_216986677.1"
                     /translation="MLFIETSIFTKQIKELVSDKEYRQLQQDLLVQPDKGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETSILRQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6118..6717
                     /locus_tag="I6L24_RS16280"
                     /old_locus_tag="I6L24_16285"
     CDS             6118..6717
                     /locus_tag="I6L24_RS16280"
                     /old_locus_tag="I6L24_16285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_216986678.1"
                     /translation="MSNYIDCSCNGDNENCFRCYGSGRINLNELDPILPIKILQNPKLE
                     LEKNFNHETYVVTKDENGEIQKKILVQCKICANQIEKDIFKEHLITCKKNKIPLKKPIY
                     NEKKEFWIKNRRGKLVCIVICNICNRRVNKENLKKHMLKVHFQVKHLNKSSLQVSSEQY
                     DDVIQDRMNDDATKLYAHSYRENGRFGSHPEHDDHE"
                     /name="hypothetical protein"
     gene            complement(7163..7354)
                     /locus_tag="I6L24_RS16285"
                     /old_locus_tag="I6L24_16290"
     CDS             complement(7163..7354)
                     /locus_tag="I6L24_RS16285"
                     /old_locus_tag="I6L24_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282089.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="WP_004282089.1"
                     /translation="MRLLIALIFPWLLFFTIGRPFAGIICLLLQITVLGWIPAAIWAVY
                     ALSQYKTDQKIKRAIRSS"
                     /name="YqaE/Pmp3 family membrane protein"
     gene            complement(7384..7665)
                     /locus_tag="I6L24_RS16290"
                     /old_locus_tag="I6L24_16295"
     CDS             complement(7384..7665)
                     /locus_tag="I6L24_RS16290"
                     /old_locus_tag="I6L24_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_216986680.1"
                     /translation="MSVTETLDSKIKAQEEKLKQLKAQRQAAVARERAKEKEQARKDDT
                     RRKILIGSCMLKITEDDEQARAKLIAQMDKYLTDERDRKLFDLPAVNY"
                     /name="mobilization protein"
     gene            7875..9311
                     /gene="mobQ"
                     /locus_tag="I6L24_RS16295"
                     /old_locus_tag="I6L24_16300"
     CDS             7875..9311
                     /gene="mobQ"
                     /locus_tag="I6L24_RS16295"
                     /old_locus_tag="I6L24_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_216986679.1"
                     /translation="MAIYHFSVKTVARSAGRSATAAIAYRAGEKIYCEREGREHDYSRK
                     TGVEYKEIYLPKDAPEHLKNRERLWNEVEQRETRKNSTVAREFEIAFPNELNQEQRLAM
                     LEELCASIVERHQVAVDACIHAPHTGSGSDERNYHAHILMSTRKLTPEGFTEKTRELDQ
                     KHSGEIDHWREHFADICNVHLDLAGSTARVDHRSYKDQENGLEATLHEGPKVTELRRRG
                     IETEISRSNDEIKQRNQAQLQYDKNMDVLIAENEIKLSTLKTEQQIQIKNSAKTPPIDE
                     KSLFEEKQRETLGKVLKREISAKDANLDLDFMQQHLEKAEITLTKHHKHQNEFNQQLAQ
                     EIVKSGLKQSHDKLQSLVDQHNELTQNKPLLFGKKAWEAQRDEIYQEHKKLKGQHEHQK
                     KHGVKDLLENKKFKEHAWKQYQQQHPAKAKQYQTLYPSYQVIKKCVDEIKAEQQMKLRQ
                     EQQLKAQQHAPKMKSRGMSR"
                     /name="mobQ"
     gene            9837..10760
                     /gene="repM"
                     /locus_tag="I6L24_RS16300"
                     /old_locus_tag="I6L24_16305"
     CDS             9837..10760
                     /gene="repM"
                     /locus_tag="I6L24_RS16300"
                     /old_locus_tag="I6L24_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282204.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_004282204.1"
                     /translation="MKTELIVKDNALINASYNLDLVEQRLILLAIVEARESGKGINAND
                     PLTVHAESYINQFGVHRNTAYQALKDACDDLFARQFSYQSLSEKGNVINHKSRWVSEVA
                     YIDNEAVVRLIFAPAIVPLITRLEEQFTKYEIQQISNLTSAYAVRLYEILIAWRSTGKT
                     PLITIYDFRQKIGVLETEYKRMYDFKKYVLDIALKQVNEHTDINVKVEQHKTGRSITGF
                     SFSFKQKKSATNTANDISQDKELKINLTDAQRYLFASKLSELPEMAKLSQGNESYEQFA
                     ARIVTMLQEPNKLKEFMPLLRKVGFQ"
                     /name="repM"
     gene            10828..11364
                     /locus_tag="I6L24_RS16305"
                     /old_locus_tag="I6L24_16310"
     CDS             10828..11364
                     /locus_tag="I6L24_RS16305"
                     /old_locus_tag="I6L24_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_216986675.1"
                     /translation="MKKLSVSELAKLYGYSRQAVYAHIKKGNLSKGSDGLIDFSEALRV
                     FGEPQKSNSSVNQSQSTSRHNLTEVDLLKRQVDMLEKQLNQAIQRENQSLERESFYQEQ
                     IEAMQRLLEAPKANMTTFTDHGSEQDIATDSRPQTESNYDGLTTPENKRIPVPEHIEPT
                     PKKRGFLSRFFLPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(11647..13101)
                     /locus_tag="I6L24_RS16310"
                     /old_locus_tag="I6L24_16315"
     CDS             complement(11647..13101)
                     /locus_tag="I6L24_RS16310"
                     /old_locus_tag="I6L24_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995702.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_131275007.1"
                     /translation="MLSNVREQWFSNIRGDVLAGLVVGLALIPEAIAFSIIAGVDPKVG
                     LYASFCIAVVISFVGGRPAMISAATGAMALLMVTLVKEHGLEYLLAATILTGIIQILAG
                     YLKLAKLMRFVSKSVVIGFVNALAILIFMAQLPELINVTWHVYALVALGLAIIYLFPLV
                     PKLGKLLPSPLVCIIAITLLAIALGIDVRTVGDMGSLPDTLPMFLIPNIPLNIETLMII
                     LPYSVALAAVGLLESMMTATIVDEMTDTPSDKYQECKGQGIANIASGFMGGMAGCAMIG
                     QSMINVKSGGLTRLSTFSAGIFLLILVVFISDWLKVIPMAALVAVMIMVSISTFEWSSV
                     TQLKDNPKSSNIVMIATVIVVVATHNLALGVLTGVLLSALFLANKLENDIQVTASFEGT
                     ARLYDLRGQIFFSSSEKFMQGFDFKEDVKDVIIDLTHSHIWDVTSVAMLDSVVNKFQKN
                     GVNVTVRGLNEASSIMIDKYGTHARI"
                     /name="SulP family inorganic anion transporter"
     gene            complement(13349..13558)
                     /locus_tag="I6L24_RS16315"
                     /old_locus_tag="I6L24_16320"
     CDS             complement(13349..13558)
                     /locus_tag="I6L24_RS16315"
                     /old_locus_tag="I6L24_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010116191.1"
                     /translation="MFNFKIFNKVSAEVLTIKNDLQLNAELQLINKYKTATSEDYKQAI
                     VLIFKERGYTRLEIGQLLGELKAS"
                     /name="hypothetical protein"
     regulatory      13757..13824
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
                     /name=""
     gene            13941..14321
                     /gene="crcB"
                     /locus_tag="I6L24_RS16320"
                     /old_locus_tag="I6L24_16325"
     CDS             13941..14321
                     /gene="crcB"
                     /locus_tag="I6L24_RS16320"
                     /old_locus_tag="I6L24_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225409.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="WP_005225409.1"
                     /translation="MYLSLLSIALGSVIGAWLRWGISLRFNSVFENIPFGTVIVNLIGA
                     FIIGLAVSFFSNSSISPNYKLFVVTGFCGALTTFSTFSVEIVALLQASKFEYAISTIAI
                     HVIGSLIFTVLGILSYQFFTNH"
                     /name="crcB"
     gene            14348..14872
                     /gene="ppa"
                     /locus_tag="I6L24_RS16325"
                     /old_locus_tag="I6L24_16330"
     CDS             14348..14872
                     /gene="ppa"
                     /locus_tag="I6L24_RS16325"
                     /old_locus_tag="I6L24_16330"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225406.1"
                     /GO_function="GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /GO_process="GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="WP_131275023.1"
                     /translation="MTFKNVKAGTNPPDDFNSIIEIPANAKPIKYEVNKEYDALLVDRF
                     LSTAMSYPYNYGYIPQTLSEDGDPLDVFVIAPHAVAVGSVIRSRPVGVMYMEDEAGIDA
                     KIIAVPHSKLTPLYNNVEDLSDLPQLQLDQMKHFFEHYKDLEKGKWMKFNGWGNVEEAR
                     QEILKSIQAYK"
                     /name="ppa"
     gene            complement(15379..15651)
                     /gene="nadS"
                     /locus_tag="I6L24_RS16330"
                     /old_locus_tag="I6L24_16335"
     CDS             complement(15379..15651)
                     /gene="nadS"
                     /locus_tag="I6L24_RS16330"
                     /old_locus_tag="I6L24_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_216986676.1"
                     /translation="MDNSLFDDLVASIKEAGAIKRNEIKASRVTELELPDIKEVREKTG
                     LTQAEFAARLHISARTLQNWEQGRRYPTGPAATLIRILDAHPTLI"
                     /name="nadS"
     gene            complement(15644..15964)
                     /locus_tag="I6L24_RS16335"
                     /old_locus_tag="I6L24_16340"
     CDS             complement(15644..15964)
                     /locus_tag="I6L24_RS16335"
                     /old_locus_tag="I6L24_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_216986677.1"
                     /translation="MLFIETSIFTKQIKELVSDKEYRQLQQDLLVQPDKGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETSILRQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            16109..16708
                     /locus_tag="I6L24_RS16340"
                     /old_locus_tag="I6L24_16345"
     CDS             16109..16708
                     /locus_tag="I6L24_RS16340"
                     /old_locus_tag="I6L24_16345"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_216986678.1"
                     /translation="MSNYIDCSCNGDNENCFRCYGSGRINLNELDPILPIKILQNPKLE
                     LEKNFNHETYVVTKDENGEIQKKILVQCKICANQIEKDIFKEHLITCKKNKIPLKKPIY
                     NEKKEFWIKNRRGKLVCIVICNICNRRVNKENLKKHMLKVHFQVKHLNKSSLQVSSEQY
                     DDVIQDRMNDDATKLYAHSYRENGRFGSHPEHDDHE"
                     /name="hypothetical protein"
     gene            complement(17154..17345)
                     /locus_tag="I6L24_RS16345"
                     /old_locus_tag="I6L24_16350"
     CDS             complement(17154..17345)
                     /locus_tag="I6L24_RS16345"
                     /old_locus_tag="I6L24_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282089.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="WP_004282089.1"
                     /translation="MRLLIALIFPWLLFFTIGRPFAGIICLLLQITVLGWIPAAIWAVY
                     ALSQYKTDQKIKRAIRSS"
                     /name="YqaE/Pmp3 family membrane protein"
     gene            complement(17375..17656)
                     /locus_tag="I6L24_RS16350"
                     /old_locus_tag="I6L24_16355"
     CDS             complement(17375..17656)
                     /locus_tag="I6L24_RS16350"
                     /old_locus_tag="I6L24_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_216986680.1"
                     /translation="MSVTETLDSKIKAQEEKLKQLKAQRQAAVARERAKEKEQARKDDT
                     RRKILIGSCMLKITEDDEQARAKLIAQMDKYLTDERDRKLFDLPAVNY"
                     /name="mobilization protein"
     gene            17866..19302
                     /gene="mobQ"
                     /locus_tag="I6L24_RS16355"
                     /old_locus_tag="I6L24_16360"
     CDS             17866..19302
                     /gene="mobQ"
                     /locus_tag="I6L24_RS16355"
                     /old_locus_tag="I6L24_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_216986679.1"
                     /translation="MAIYHFSVKTVARSAGRSATAAIAYRAGEKIYCEREGREHDYSRK
                     TGVEYKEIYLPKDAPEHLKNRERLWNEVEQRETRKNSTVAREFEIAFPNELNQEQRLAM
                     LEELCASIVERHQVAVDACIHAPHTGSGSDERNYHAHILMSTRKLTPEGFTEKTRELDQ
                     KHSGEIDHWREHFADICNVHLDLAGSTARVDHRSYKDQENGLEATLHEGPKVTELRRRG
                     IETEISRSNDEIKQRNQAQLQYDKNMDVLIAENEIKLSTLKTEQQIQIKNSAKTPPIDE
                     KSLFEEKQRETLGKVLKREISAKDANLDLDFMQQHLEKAEITLTKHHKHQNEFNQQLAQ
                     EIVKSGLKQSHDKLQSLVDQHNELTQNKPLLFGKKAWEAQRDEIYQEHKKLKGQHEHQK
                     KHGVKDLLENKKFKEHAWKQYQQQHPAKAKQYQTLYPSYQVIKKCVDEIKAEQQMKLRQ
                     EQQLKAQQHAPKMKSRGMSR"
                     /name="mobQ"
     Typing          2..769
                     /name="rep_cluster_1254"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.74"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9838..10760
                     /name="rep_cluster_1254"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.784"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7876..9308
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.749"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          17867..19299
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.749"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagttatatc aatcaatttg gtgtacatcg aaatactgct tatcaagcct taaaagatgc
       61 ttgcgatgac ctatttgcaa gacaattcag ttatcaaagt cttagtgaaa aaggtaatgt
      121 cattaatcac aagtcaagat gggtaagcga ggttgcttat attgataatg aggctgtcgt
      181 tagacttatt tttgctccag ctattgtgcc tttaataact cgattagaag aacaatttac
      241 aaagtatgag atacagcaaa taagcaattt aacgagtgct tatgctgttc gcttatatga
      301 aatattgatt gcgtggcgta gtaccggaaa aacgcctctt ataactatct acgatttcag
      361 acaaaaaata ggtgtactcg agactgaata caaaaggatg tacgatttta agaaatatgt
      421 tttagacatt gcattaaagc aagtaaatga acataccgat attaatgtca aagttgaaca
      481 acataaaacc ggcagatcca ttacgggctt ttcatttagc ttcaaacaga aaaagtcagc
      541 aacaaataca gccaatgaca ttagtcagga caaagagcta aaaatcaatt tgactgatgc
      601 acaacgatat ttatttgcta gtaaattatc agaacttcct gagatggcta aactttcaca
      661 aggtaatgaa agctatgaac agtttgctgc tcggattgta accatgctgc aagagccaaa
      721 taaattaaaa gaatttatgc cattacttcg aaaagtcggc tttcaatgaa tgatgtcaat
      781 tgacatagta aagttaacaa tcaactatta acactatttg aaaactggtt tacataatga
      841 aaaaactgtc agtttcagaa ctagcgaaat tatatggata ttcaagacaa gctgtatatg
      901 cccatataaa gaaaggaaat ttatcaaaag gatcggatgg attaattgac ttttcagaag
      961 ccttgagagt ttttggggaa ccacaaaaaa gcaattctag tgtcaaccaa agtcaatcaa
     1021 ctagtaggca taacttaaca gaagttgact tgctaaaacg tcaagttgac atgctggaaa
     1081 aacaattaaa tcaggcaata cagagagaaa atcaatcatt agaacgtgaa tcgttttatc
     1141 aagaacagat tgaggccatg cagcgcttac tggaagctcc aaaagccaat atgactacgt
     1201 ttaccgatca tggatctgaa caggatatag caacagattc tcggccacag actgaatcga
     1261 actatgacgg attgactact ccagagaaca aacgcatccc tgttccagaa catattgagc
     1321 ctacacctaa aaagaggggc ttcctgagtc gttttttcct tccttacggt taaccaggac
     1381 tccacttttt tgatgagcca atttcagtgc aagcacatga aataatgact cctcaaaaaa
     1441 ccggatgagg tttattaaaa ctgaacaaaa ccgtggaaca ttgcttaaat agtaagcgtg
     1501 gaacatgaaa aatcattaaa aaagcccaac tgagtgcagt tgggcttttt tgtatacgat
     1561 aagactatga tttatataga ataaaaaaga gcatttcgta taaggtgtat tatgttaatt
     1621 ttagagattc tacataaatg tagagtctcc ttcacttaga ttctggcatg tgttccatac
     1681 ttatcaatca tgatagagct agcttcattt aagccacgca cagtgacatt cacaccattc
     1741 ttttgaaatt tatttacaac agaatcaagc atggcgactg atgttacgtc ccaaatgtga
     1801 gaatgggtca aatcaataat cacatccttt acatcctctt taaagtcaaa accttgcatg
     1861 aatttttcag aagaactaaa gaagatctga cctctcaaat catacaaacg ggccgttccc
     1921 tcaaaagaag ctgtcacctg gatatcattt tcaagtttat tcgccaagaa caaggctgaa
     1981 agcaatacgc ccgttaatac acctagagcc aagttatgcg tagcaacaac tacaatcact
     2041 gtcgcaatca tgacgatatt actactctta ggattatctt ttaactgagt gacagaactc
     2101 cattcaaaag tactaataga gaccatgatc atcaccgcta ccaatgcagc cattggaatc
     2161 actttgagcc agtcactaat aaatacaacc agaataagaa gaaaaatacc cgcagagaat
     2221 gttgataagc gtgtaaggcc gcctgatttt acgttaatca tagattgacc aatcatggca
     2281 caaccggcca tgccacccat aaagccagaa gcaatattcg caatcccttg tcccttacat
     2341 tcctgatatt tatcacttgg cgtatctgtc atttcatcca cgatggttgc tgtcatcatc
     2401 gattcaagta aacctacagc cgccagtgct accgagtaag gcaaaataat catcaatgtt
     2461 tcaatattca aaggaatatt tggaatcaaa aacattggaa gtgtgtcagg taaagacccc
     2521 atatcaccca cagtccgtac atcaattcct aaagcgattg ccagtaatgt gatcgcaatg
     2581 atgcagacaa gtggagaggg gagtaatttt cctaatttgg gaaccaaagg aaagagataa
     2641 ataatcgcca gacctaaagc aaccagagca tatacatgcc aggtcacatt aatcagttct
     2701 ggtaactgag ccatgaaaat taagatcgct aacgcattta cgaatccaat aactacagat
     2761 ttagatacga accgcattaa cttggcgagc tttaaatagc ctgccaatat ctgaattatg
     2821 cctgtcagaa ttgttgctgc cagcagatat tcaagcccat gctctttgac caatgtgacc
     2881 ataagcagtg ccatagcccc tgtagcagct gaaatcattg ctggacgacc acctacgaaa
     2941 gaaatcacga cagcaataca gaaggatgcg tatagaccga ctttaggatc tacacctgca
     3001 ataattgaaa atgcgatggc ttcagggatg agtgcaagac cgacgactaa tccggcaagt
     3061 acatccccac ggatgttaga gaaccactgt tctcggacat tagataacat aaacatagcc
     3121 tgttttttaa tttttcagat aggccagtga tgtgatatat acaacacaca agcgactaca
     3181 ccacatcact agacagtgat gagttattta cagatttaag agggtaaata aacttaaggt
     3241 ggcgtaagcg tcataagatg gaggaacttt ttagagtaaa aaatcaggac aagggtaaca
     3301 gctaggtact tttttataaa gcctggatgt tataccaagc tttgtttaat cggtatttta
     3361 tgaagctttt agctccccaa gtaattgacc tatttccaaa cgtgtatagc cacgttcttt
     3421 aaagatcaaa acaatagcct gtttataatc ttcagaagtg gccgttttat atttattaat
     3481 taattgcagc tcagcattaa gctgaaggtc atttttgatg gtcaatactt ctgcagagac
     3541 tttattaaaa atcttgaaat taaacataca ggtgctcttt atcaatattg tagcggcccg
     3601 actacaacaa acacctgcct cagcttagct ggacaccctt tccaaaaaga ttatagaaaa
     3661 aatgaaaagt ttcaagtttt actaaactaa agataaaaaa gtatcaaact tacgtatttt
     3721 aataatagaa ggttgttgaa ttagctaatc aatttttaaa taatagtact caaaggagat
     3781 ggctatcctc cttacacaaa ccgccaattc tggctgatga tacctacgtt tccctcaagc
     3841 agggtacgta gtcgcgtgct ctgcttttac aaaatggagc tacgctatgt tttcccgtca
     3901 taaagttctt tatttatata tcttttgctg ctcataacta ggagcagaca tgtatctttc
     3961 tcttttatca attgctctcg gttctgtaat cggtgcctgg cttcgttggg gcataagtct
     4021 aagatttaat agcgtatttg aaaatattcc tttcgggaca gtgattgtta acttaatcgg
     4081 tgcttttatt attggattag ctgtatcatt tttttcaaat agctcaataa gccctaatta
     4141 taagttattc gttgtgacag gcttctgcgg tgctttaacg accttttcaa cattttcagt
     4201 tgaaattgtt gcgttgctac aagcttcaaa gtttgaatat gctatctcaa caatagccat
     4261 ccatgtaata ggttcattaa tctttacagt actaggaata ctttcctatc aatttttcac
     4321 taatcattaa taatctaaat attagaaggt aaaacaatga cttttaaaaa tgttaaagca
     4381 ggtactaatc caccagatga ctttaatagc atcatcgaaa tcccggcaaa tgctaaaccc
     4441 atcaagtatg aagtgaataa agaatatgat gctttattgg ttgatcgctt tctaagtaca
     4501 gcaatgtcat atccgtacaa ttacggttat attcctcaaa cattaagcga ggatggtgat
     4561 cccttagatg tatttgtaat agctccccat gcggttgctg taggatcagt aattcgcagc
     4621 agacctgtcg gagtcatgta tatggaagat gaagccggta ttgatgcaaa gatcattgct
     4681 gtacctcatt ctaagctcac acctctatat aacaatgttg aagatctgag cgacctccct
     4741 cagcttcaat tagatcaaat gaaacatttc tttgaacatt ataaagactt ggaaaaaggt
     4801 aaatggatga aatttaatgg ttggggtaat gtagaagaag ccagacagga gattctaaaa
     4861 tctatccaag catacaaata atttagataa aagggaccca aatctgaggt ccctttttat
     4921 gctatttaac ataatggcgg ttatacgaag tacacttgta tattaactta aatatcaatt
     4981 acttaaaaaa ttaatcctga tcataaaaac cgcaaaaagc cgattttgaa acaagttggc
     5041 ttttttatga gcggttttga tacttcttgc caataaaatt gtttaaaaaa tagtcaattt
     5101 tgcgattttt taaattttat caatgcttga atgcttaaaa ttatccacat tttttgtgga
     5161 taaaatttag gctattttgt acgctttagc tcacatcaat ctcggccctt tgcggctatc
     5221 cggcatactg gaattttctt atgatgggta catagagaac aggatgttcc aaataacaag
     5281 aataagaaag atcgcaccag gaacgtgagg taagacagga gtcataccta gcaacccaat
     5341 atgaaaaacc ccggcaggat gccggggttt tttattatct aaacttttta aattagggtt
     5401 ggatgggcat ccagaatgcg aattagagtc gctgcagggc cagtcggata gcgacgacct
     5461 tgctcccagt tttgcagggt tctcgcgctg atatgcaaac gtgcagcaaa ttctgcctgg
     5521 gttaagccag ttttctcacg tacttcctta atatccggca attcaagctc tgtgactcga
     5581 cttgccttta tctcattacg tttgatagca ccagcttctt taattgaagc aaccaggtca
     5641 tcaaataagc tgttatccat gaaattgctc cttcactaat tgacgcagga tcgaggtctc
     5701 tttatcagtc agattgtctt tcacagactt tggataagct accaagaaaa agatctgatc
     5761 atcttctgtg acccaatagt agatcacacg gataccgcca cttttaccct tgttaccttg
     5821 agcacaacgt actttacgaa tacctccgcc attcttgatt aagtcgccct tatcaggctg
     5881 taccaagaga tcttgctgga gctgacgata ctctttatca ctcacaagtt ctttgatttg
     5941 cttggtaaaa atactggttt caatgaataa cataggttga agtacgtcaa tggcgtataa
     6001 gaattttagc agttctaaaa caaaaagacg agaactatga aatctcgttt ttgtgtaatc
     6061 tgtattatat taatttgaaa tgaaacttta ttattatatt tttaagggga ataaattatg
     6121 agtaattata tagactgctc atgtaacgga gataatgaaa attgctttag atgttatggc
     6181 tcaggacgaa ttaatctgaa tgaattagac ccaatattac ctataaaaat tttgcaaaat
     6241 ccaaaattgg aacttgaaaa aaatttcaat catgaaactt atgtagtgac taaagatgaa
     6301 aatggagaaa tacaaaaaaa aatattagtt caatgtaaaa tttgtgcaaa tcaaatcgaa
     6361 aaagatattt tcaaggagca tttaattaca tgtaaaaaaa ataaaattcc tctaaaaaaa
     6421 cctatatata atgaaaaaaa agaattttgg ataaaaaata gaaggggaaa gctagtctgt
     6481 atcgttatat gtaatatatg caatagaaga gtgaataaag aaaatctaaa aaaacatatg
     6541 ctaaaagtac acttccaagt taaacattta aataaaagta gcctacaagt ttcttcagaa
     6601 caatatgatg atgttattca agatagaatg aatgacgatg ctacaaaatt atatgcacat
     6661 tcctatagag agaatggacg gtttggatct cacccagaac atgatgatca tgaataagat
     6721 ttctactaag ctctataata tactttctgt tcaataattt ttaacgtgga tgccgctcta
     6781 caaggttttt tagccaattt taagaaaggc ttattagaag aatttatcaa ttttttagac
     6841 gtatggtttc tgagttagaa agtcgctgta ttgattctat caaatttttc agtaacttcc
     6901 gaatcctaat gtttcgcttt atggcaaaaa atagagtgaa ctcgataaaa aaatgaaacc
     6961 ctctaattct tgttttaatt atttttctaa ctaagaatta gagggttaat ttctttacct
     7021 ctggagtttt ataattattg ttataaaatt tattaaatca cttattgctc aaataatcct
     7081 cacaatctga tcatttactc atggataagt gtgatggaaa tcacaaaaac gatcagatct
     7141 aaaaatcaca caattacact atttatgatg agcgaattgc tcttttaatt ttctgatcag
     7201 tcttgtattg cgacaaagca tagaccgccc agatggcagc cgggatccaa cctaaaactg
     7261 taatctgaag tagcaaacaa atgatgcctg caaatggtcg tcctatggtg aaaaacagca
     7321 gccatggaaa aattaaagca attagcaatc tcatttcaaa ttcctatgta tcttttcaaa
     7381 aaattaataa ttaactgccg gcagatcaaa cagcttacga tctctttcat ccgttaaata
     7441 cttatccatc tgagcaatta atttagctct ggcttgttca tcatcttctg tgatttttaa
     7501 catgcaggat ccgattaaaa tcttacgtct ggtatcgtcc tttcttgcct gttctttctc
     7561 tttggctcgt tctcttgcta ctgctgcctg tctttgagcc tttaactgct ttagcttttc
     7621 ttcctgtgcc ttgattttgc tgtctagtgt ttcagtaacg ctcattaatc caataccatc
     7681 atcctgggaa accatcccta acataagcgc acttgaatat gtattcaagg tgctatatgt
     7741 atagtaatgt tttcctcgaa gagcgcactt atgcaaactt cgtttgcaag tacgattggg
     7801 gagtccccaa taccccgaca accgtatcca cggttgtatt tcactacgtg aaaatttaaa
     7861 agcgaaaaac aggcatggcg atttaccact tttcagtcaa aaccgtagca cgatcagcag
     7921 ggcgttccgc cactgctgca atcgcctatc gggccggtga aaagatctat tgtgaacgtg
     7981 aaggccgaga acatgactac agccgaaaaa ccggtgtgga atataaagaa atttatttac
     8041 ccaaagacgc accagagcat ttaaaaaacc gggaaagact ctggaatgaa gtagaacaac
     8101 gagaaacccg aaaaaactcg acagttgcgc gggaatttga aatcgctttt ccaaatgaat
     8161 taaatcagga acaacgcctg gccatgctcg aagaattatg tgccagcatc gtggaaagac
     8221 atcaggtagc agtcgatgcc tgtattcatg caccgcatac cggatccggc agtgatgagc
     8281 gcaattatca cgcccatatc ctaatgagca cacgtaaact tacgccagaa ggttttacag
     8341 agaaaacccg ggaactcgat cagaaacaca gtggagaaat cgatcactgg cgtgaacact
     8401 ttgccgatat ctgcaatgtg cacctggatc tggccggatc cacagcccgg gtcgaccacc
     8461 gcagctacaa agaccaggag aatggcctgg aagccaccct gcacgaagga ccgaaagtca
     8521 ccgaactacg tagaagaggc atcgaaaccg aaatcagccg aagcaatgat gaaatcaaac
     8581 agagaaatca ggctcagttg caatacgaca aaaatatgga cgttctaatt gctgaaaatg
     8641 agataaaact cagcacattg aaaacagaac agcagatcca aatcaagaat agcgctaaaa
     8701 cgccaccaat tgacgaaaaa tccctgtttg aggaaaaaca gagggaaacc cttggcaaag
     8761 tgctaaaacg cgaaatcagc gcaaaagacg cgaatctaga cctggatttc atgcagcagc
     8821 atctagagaa ggccgaaata accctgacca agcaccacaa acatcaaaac gagttcaatc
     8881 agcagcttgc ccaggagatc gtgaaaagcg gactcaagca aagtcatgac aaattgcaaa
     8941 gcctggtcga tcaacacaac gaattaaccc agaacaaacc cttactgttt ggaaaaaaag
     9001 cctgggaagc ccaacgtgat gagatctacc aggaacacaa aaagctgaaa ggtcagcatg
     9061 aacaccagaa aaaacatggt gtgaaggatt tattggagaa taaaaagttc aaagaacatg
     9121 cctggaagca gtaccaacaa caacatccgg ccaaagccaa gcaataccag actctatacc
     9181 catcctatca agtcattaaa aaatgtgtgg atgagatcaa agcagaacaa cagatgaaac
     9241 tcagacaaga acaacagctc aaagcacagc aacatgcgcc taaaatgaaa tctcgtggca
     9301 tgagtcgata aacagagcga gtgtctacga gcgaactgaa aaaattcgcc cctttctttc
     9361 cctgattaca agctccctat ccatccatca cctgcatggc agagcatccc gcatgggtgc
     9421 gaactttcca aattggcttc ccaaaccgag cgaagtgagt aaaaaagctc atgagcgaag
     9481 cgaattccga gctgcttttg ctttttctta aattcacgag aatatgaacc aaaaattgcc
     9541 ccgacgaatc gagcgaaagc gagattcaat agagtttgag cgaagcgaaa accaagggca
     9601 atttttcatg aaatatgctt ttaattattt ttaagctttt aaatgctttt agatagcctg
     9661 taaacctgaa tttataaggc tttgagagat tataaaacga ggtttacctt gcattaaaac
     9721 gaggtttacc ttgcattaaa acgaggttta ccttgcatta aaacgaggtt taccttgcat
     9781 taagcgagtt gataatataa cctcgtctta ttgacttaaa aatataaatt tttcatatga
     9841 aaaccgaact aattgtcaaa gataacgctt taattaatgc tagttataac cttgatctag
     9901 tagaacaacg attaattctt ttagctatcg ttgaagccag ggaatcaggt aaagggataa
     9961 atgctaatga tccattaaca gttcatgctg aaagttatat caatcaattt ggtgtacatc
    10021 gaaatactgc ttatcaagcc ttaaaagatg cttgcgatga cctatttgca agacaattca
    10081 gttatcaaag tcttagtgaa aaaggtaatg tcattaatca caagtcaaga tgggtaagcg
    10141 aggttgctta tattgataat gaggctgtcg ttagacttat ttttgctcca gctattgtgc
    10201 ctttaataac tcgattagaa gaacaattta caaagtatga gatacagcaa ataagcaatt
    10261 taacgagtgc ttatgctgtt cgcttatatg aaatattgat tgcgtggcgt agtaccggaa
    10321 aaacgcctct tataactatc tacgatttca gacaaaaaat aggtgtactc gagactgaat
    10381 acaaaaggat gtacgatttt aagaaatatg ttttagacat tgcattaaag caagtaaatg
    10441 aacataccga tattaatgtc aaagttgaac aacataaaac cggcagatcc attacgggct
    10501 tttcatttag cttcaaacag aaaaagtcag caacaaatac agccaatgac attagtcagg
    10561 acaaagagct aaaaatcaat ttgactgatg cacaacgata tttatttgct agtaaattat
    10621 cagaacttcc tgagatggct aaactttcac aaggtaatga aagctatgaa cagtttgctg
    10681 ctcggattgt aaccatgctg caagagccaa ataaattaaa agaatttatg ccattacttc
    10741 gaaaagtcgg ctttcaatga atgatgtcaa ttgacatagt aaagttaaca atcaactatt
    10801 aacactattt gaaaactggt ttacataatg aaaaaactgt cagtttcaga actagcgaaa
    10861 ttatatggat attcaagaca agctgtatat gcccatataa agaaaggaaa tttatcaaaa
    10921 ggatcggatg gattaattga cttttcagaa gccttgagag tttttgggga accacaaaaa
    10981 agcaattcta gtgtcaacca aagtcaatca actagtaggc ataacttaac agaagttgac
    11041 ttgctaaaac gtcaagttga catgctggaa aaacaattaa atcaggcaat acagagagaa
    11101 aatcaatcat tagaacgtga atcgttttat caagaacaga ttgaggccat gcagcgctta
    11161 ctggaagctc caaaagccaa tatgactacg tttaccgatc atggatctga acaggatata
    11221 gcaacagatt ctcggccaca gactgaatcg aactatgacg gattgactac tccagagaac
    11281 aaacgcatcc ctgttccaga acatattgag cctacaccta aaaagagggg cttcctgagt
    11341 cgttttttcc ttccttacgg ttaaccagga ctccactttt ttgatgagcc aatttcagtg
    11401 caagcacatg aaataatgac tcctcaaaaa accggatgag gtttattaaa actgaacaaa
    11461 accgtggaac attgcttaaa tagtaagcgt ggaacatgaa aaatcattaa aaaagcccaa
    11521 ctgagtgcag ttgggctttt ttgtatacga taagactatg atttatatag aataaaaaag
    11581 agcatttcgt ataaggtgta ttatgttaat tttagagatt ctacataaat gtagagtctc
    11641 cttcacttag attctggcat gtgttccata cttatcaatc atgatagagc tagcttcatt
    11701 taagccacgc acagtgacat tcacaccatt cttttgaaat ttatttacaa cagaatcaag
    11761 catggcgact gatgttacgt cccaaatgtg agaatgggtc aaatcaataa tcacatcctt
    11821 tacatcctct ttaaagtcaa aaccttgcat gaatttttca gaagaactaa agaagatctg
    11881 acctctcaaa tcatacaaac gggccgttcc ctcaaaagaa gctgtcacct ggatatcatt
    11941 ttcaagttta ttcgccaaga acaaggctga aagcaatacg cccgttaata cacctagagc
    12001 caagttatgc gtagcaacaa ctacaatcac tgtcgcaatc atgacgatat tactactctt
    12061 aggattatct tttaactgag tgacagaact ccattcaaaa gtactaatag agaccatgat
    12121 catcaccgct accaatgcag ccattggaat cactttgagc cagtcactaa taaatacaac
    12181 cagaataaga agaaaaatac ccgcagagaa tgttgataag cgtgtaaggc cgcctgattt
    12241 tacgttaatc atagattgac caatcatggc acaaccggcc atgccaccca taaagccaga
    12301 agcaatattc gcaatccctt gtcccttaca ttcctgatat ttatcacttg gcgtatctgt
    12361 catttcatcc acgatggttg ctgtcatcat cgattcaagt aaacctacag ccgccagtgc
    12421 taccgagtaa ggcaaaataa tcatcaatgt ttcaatattc aaaggaatat ttggaatcaa
    12481 aaacattgga agtgtgtcag gtaaagaccc catatcaccc acagtccgta catcaattcc
    12541 taaagcgatt gccagtaatg tgatcgcaat gatgcagaca agtggagagg ggagtaattt
    12601 tcctaatttg ggaaccaaag gaaagagata aataatcgcc agacctaaag caaccagagc
    12661 atatacatgc caggtcacat taatcagttc tggtaactga gccatgaaaa ttaagatcgc
    12721 taacgcattt acgaatccaa taactacaga tttagatacg aaccgcatta acttggcgag
    12781 ctttaaatag cctgccaata tctgaattat gcctgtcaga attgttgctg ccagcagata
    12841 ttcaagccca tgctctttga ccaatgtgac cataagcagt gccatagccc ctgtagcagc
    12901 tgaaatcatt gctggacgac cacctacgaa agaaatcacg acagcaatac agaaggatgc
    12961 gtatagaccg actttaggat ctacacctgc aataattgaa aatgcgatgg cttcagggat
    13021 gagtgcaaga ccgacgacta atccggcaag tacatcccca cggatgttag agaaccactg
    13081 ttctcggaca ttagataaca taaacatagc ctgtttttta atttttcaga taggccagtg
    13141 atgtgatata tacaacacac aagcgactac accacatcac tagacagtga tgagttattt
    13201 acagatttaa gagggtaaat aaacttaagg tggcgtaagc gtcataagat ggaggaactt
    13261 tttagagtaa aaaatcagga caagggtaac agctaggtac ttttttataa agcctggatg
    13321 ttataccaag ctttgtttaa tcggtatttt atgaagcttt tagctcccca agtaattgac
    13381 ctatttccaa acgtgtatag ccacgttctt taaagatcaa aacaatagcc tgtttataat
    13441 cttcagaagt ggccgtttta tatttattaa ttaattgcag ctcagcatta agctgaaggt
    13501 catttttgat ggtcaatact tctgcagaga ctttattaaa aatcttgaaa ttaaacatac
    13561 aggtgctctt tatcaatatt gtagcggccc gactacaaca aacacctgcc tcagcttagc
    13621 tggacaccct ttccaaaaag attatagaaa aaatgaaaag tttcaagttt tactaaacta
    13681 aagataaaaa agtatcaaac ttacgtattt taataataga aggttgttga attagctaat
    13741 caatttttaa ataatagtac tcaaaggaga tggctatcct ccttacacaa accgccaatt
    13801 ctggctgatg atacctacgt ttccctcaag cagggtacgt agtcgcgtgc tctgctttta
    13861 caaaatggag ctacgctatg ttttcccgtc ataaagttct ttatttatat atcttttgct
    13921 gctcataact aggagcagac atgtatcttt ctcttttatc aattgctctc ggttctgtaa
    13981 tcggtgcctg gcttcgttgg ggcataagtc taagatttaa tagcgtattt gaaaatattc
    14041 ctttcgggac agtgattgtt aacttaatcg gtgcttttat tattggatta gctgtatcat
    14101 ttttttcaaa tagctcaata agccctaatt ataagttatt cgttgtgaca ggcttctgcg
    14161 gtgctttaac gaccttttca acattttcag ttgaaattgt tgcgttgcta caagcttcaa
    14221 agtttgaata tgctatctca acaatagcca tccatgtaat aggttcatta atctttacag
    14281 tactaggaat actttcctat caatttttca ctaatcatta ataatctaaa tattagaagg
    14341 taaaacaatg acttttaaaa atgttaaagc aggtactaat ccaccagatg actttaatag
    14401 catcatcgaa atcccggcaa atgctaaacc catcaagtat gaagtgaata aagaatatga
    14461 tgctttattg gttgatcgct ttctaagtac agcaatgtca tatccgtaca attacggtta
    14521 tattcctcaa acattaagcg aggatggtga tcccttagat gtatttgtaa tagctcccca
    14581 tgcggttgct gtaggatcag taattcgcag cagacctgtc ggagtcatgt atatggaaga
    14641 tgaagccggt attgatgcaa agatcattgc tgtacctcat tctaagctca cacctctata
    14701 taacaatgtt gaagatctga gcgacctccc tcagcttcaa ttagatcaaa tgaaacattt
    14761 ctttgaacat tataaagact tggaaaaagg taaatggatg aaatttaatg gttggggtaa
    14821 tgtagaagaa gccagacagg agattctaaa atctatccaa gcatacaaat aatttagata
    14881 aaagggaccc aaatctgagg tcccttttta tgctatttaa cataatggcg gttatacgaa
    14941 gtacacttgt atattaactt aaatatcaat tacttaaaaa attaatcctg atcataaaaa
    15001 ccgcaaaaag ccgattttga aacaagttgg cttttttatg agcggttttg atacttcttg
    15061 ccaataaaat tgtttaaaaa atagtcaatt ttgcgatttt ttaaatttta tcaatgcttg
    15121 aatgcttaaa attatccaca ttttttgtgg ataaaattta ggctattttg tacgctttag
    15181 ctcacatcaa tctcggccct ttgcggctat ccggcatact ggaattttct tatgatgggt
    15241 acatagagaa caggatgttc caaataacaa gaataagaaa gatcgcacca ggaacgtgag
    15301 gtaagacagg agtcatacct agcaacccaa tatgaaaaac cccggcagga tgccggggtt
    15361 ttttattatc taaacttttt aaattagggt tggatgggca tccagaatgc gaattagagt
    15421 cgctgcaggg ccagtcggat agcgacgacc ttgctcccag ttttgcaggg ttctcgcgct
    15481 gatatgcaaa cgtgcagcaa attctgcctg ggttaagcca gttttctcac gtacttcctt
    15541 aatatccggc aattcaagct ctgtgactcg acttgccttt atctcattac gtttgatagc
    15601 accagcttct ttaattgaag caaccaggtc atcaaataag ctgttatcca tgaaattgct
    15661 ccttcactaa ttgacgcagg atcgaggtct ctttatcagt cagattgtct ttcacagact
    15721 ttggataagc taccaagaaa aagatctgat catcttctgt gacccaatag tagatcacac
    15781 ggataccgcc acttttaccc ttgttacctt gagcacaacg tactttacga atacctccgc
    15841 cattcttgat taagtcgccc ttatcaggct gtaccaagag atcttgctgg agctgacgat
    15901 actctttatc actcacaagt tctttgattt gcttggtaaa aatactggtt tcaatgaata
    15961 acataggttg aagtacgtca atggcgtata agaattttag cagttctaaa acaaaaagac
    16021 gagaactatg aaatctcgtt tttgtgtaat ctgtattata ttaatttgaa atgaaacttt
    16081 attattatat ttttaagggg aataaattat gagtaattat atagactgct catgtaacgg
    16141 agataatgaa aattgcttta gatgttatgg ctcaggacga attaatctga atgaattaga
    16201 cccaatatta cctataaaaa ttttgcaaaa tccaaaattg gaacttgaaa aaaatttcaa
    16261 tcatgaaact tatgtagtga ctaaagatga aaatggagaa atacaaaaaa aaatattagt
    16321 tcaatgtaaa atttgtgcaa atcaaatcga aaaagatatt ttcaaggagc atttaattac
    16381 atgtaaaaaa aataaaattc ctctaaaaaa acctatatat aatgaaaaaa aagaattttg
    16441 gataaaaaat agaaggggaa agctagtctg tatcgttata tgtaatatat gcaatagaag
    16501 agtgaataaa gaaaatctaa aaaaacatat gctaaaagta cacttccaag ttaaacattt
    16561 aaataaaagt agcctacaag tttcttcaga acaatatgat gatgttattc aagatagaat
    16621 gaatgacgat gctacaaaat tatatgcaca ttcctataga gagaatggac ggtttggatc
    16681 tcacccagaa catgatgatc atgaataaga tttctactaa gctctataat atactttctg
    16741 ttcaataatt tttaacgtgg atgccgctct acaaggtttt ttagccaatt ttaagaaagg
    16801 cttattagaa gaatttatca attttttaga cgtatggttt ctgagttaga aagtcgctgt
    16861 attgattcta tcaaattttt cagtaacttc cgaatcctaa tgtttcgctt tatggcaaaa
    16921 aatagagtga actcgataaa aaaatgaaac cctctaattc ttgttttaat tatttttcta
    16981 actaagaatt agagggttaa tttctttacc tctggagttt tataattatt gttataaaat
    17041 ttattaaatc acttattgct caaataatcc tcacaatctg atcatttact catggataag
    17101 tgtgatggaa atcacaaaaa cgatcagatc taaaaatcac acaattacac tatttatgat
    17161 gagcgaattg ctcttttaat tttctgatca gtcttgtatt gcgacaaagc atagaccgcc
    17221 cagatggcag ccgggatcca acctaaaact gtaatctgaa gtagcaaaca aatgatgcct
    17281 gcaaatggtc gtcctatggt gaaaaacagc agccatggaa aaattaaagc aattagcaat
    17341 ctcatttcaa attcctatgt atcttttcaa aaaattaata attaactgcc ggcagatcaa
    17401 acagcttacg atctctttca tccgttaaat acttatccat ctgagcaatt aatttagctc
    17461 tggcttgttc atcatcttct gtgattttta acatgcagga tccgattaaa atcttacgtc
    17521 tggtatcgtc ctttcttgcc tgttctttct ctttggctcg ttctcttgct actgctgcct
    17581 gtctttgagc ctttaactgc tttagctttt cttcctgtgc cttgattttg ctgtctagtg
    17641 tttcagtaac gctcattaat ccaataccat catcctggga aaccatccct aacataagcg
    17701 cacttgaata tgtattcaag gtgctatatg tatagtaatg ttttcctcga agagcgcact
    17761 tatgcaaact tcgtttgcaa gtacgattgg ggagtcccca ataccccgac aaccgtatcc
    17821 acggttgtat ttcactacgt gaaaatttaa aagcgaaaaa caggcatggc gatttaccac
    17881 ttttcagtca aaaccgtagc acgatcagca gggcgttccg ccactgctgc aatcgcctat
    17941 cgggccggtg aaaagatcta ttgtgaacgt gaaggccgag aacatgacta cagccgaaaa
    18001 accggtgtgg aatataaaga aatttattta cccaaagacg caccagagca tttaaaaaac
    18061 cgggaaagac tctggaatga agtagaacaa cgagaaaccc gaaaaaactc gacagttgcg
    18121 cgggaatttg aaatcgcttt tccaaatgaa ttaaatcagg aacaacgcct ggccatgctc
    18181 gaagaattat gtgccagcat cgtggaaaga catcaggtag cagtcgatgc ctgtattcat
    18241 gcaccgcata ccggatccgg cagtgatgag cgcaattatc acgcccatat cctaatgagc
    18301 acacgtaaac ttacgccaga aggttttaca gagaaaaccc gggaactcga tcagaaacac
    18361 agtggagaaa tcgatcactg gcgtgaacac tttgccgata tctgcaatgt gcacctggat
    18421 ctggccggat ccacagcccg ggtcgaccac cgcagctaca aagaccagga gaatggcctg
    18481 gaagccaccc tgcacgaagg accgaaagtc accgaactac gtagaagagg catcgaaacc
    18541 gaaatcagcc gaagcaatga tgaaatcaaa cagagaaatc aggctcagtt gcaatacgac
    18601 aaaaatatgg acgttctaat tgctgaaaat gagataaaac tcagcacatt gaaaacagaa
    18661 cagcagatcc aaatcaagaa tagcgctaaa acgccaccaa ttgacgaaaa atccctgttt
    18721 gaggaaaaac agagggaaac ccttggcaaa gtgctaaaac gcgaaatcag cgcaaaagac
    18781 gcgaatctag acctggattt catgcagcag catctagaga aggccgaaat aaccctgacc
    18841 aagcaccaca aacatcaaaa cgagttcaat cagcagcttg cccaggagat cgtgaaaagc
    18901 ggactcaagc aaagtcatga caaattgcaa agcctggtcg atcaacacaa cgaattaacc
    18961 cagaacaaac ccttactgtt tggaaaaaaa gcctgggaag cccaacgtga tgagatctac
    19021 caggaacaca aaaagctgaa aggtcagcat gaacaccaga aaaaacatgg tgtgaaggat
    19081 ttattggaga ataaaaagtt caaagaacat gcctggaagc agtaccaaca acaacatccg
    19141 gccaaagcca agcaatacca gactctatac ccatcctatc aagtcattaa aaaatgtgtg
    19201 gatgagatca aagcagaaca acagatgaaa ctcagacaag aacaacagct caaagcacag
    19261 caacatgcgc ctaaaatgaa atctcgtggc atgagtcgat aaacagagcg agtgtctacg
    19321 agcgaactga aaaaattcgc ccctttcttt ccctgattac aagctcccta tccatccatc
    19381 acctgcatgg cagagcatcc cgcatgggtg cgaactttcc aaattggctt cccaaaccga
    19441 gcgaagtgag taaaaaagct catgagcgaa gcgaattccg agctgctttt gctttttctt
    19501 aaattcacga gaatatgaac caaaaattgc cccgacgaat cgagcgaaag cgagattcaa
    19561 tagagtttga gcgaagcgaa aaccaagggc aatttttcat gaaatatgct tttaattatt
    19621 tttaagcttt taaatgcttt tagatagcct gtaaacctga atttataagg ctttgagaga
    19681 ttataaaacg aggtttacct tgcattaaaa cgaggtttac cttgcattaa aacgaggttt
    19741 accttgcatt aaaacgaggt ttaccttgca ttaagcgagt tgataatata acctcgtctt
    19801 attgacttaa aaatataaat ttttcatatg aaaaccgaac taattgtcaa agataacgct
    19861 ttaattaatg ctagttataa ccttgatcta gtagaacaac gattaattct tttagctatc
    19921 gttgaagcca gggaatcagg taaagggata aatgctaatg atccattaac agttcatgct
    19981 ga
//
