LOCUS       NZ_CP077307             5594 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter pittii strain FDAARGOS 1396 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP077307
VERSION     NZ_CP077307.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357538
            Assembly: GCF_019048225.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 9.0.0, HGAP v. 4.0
            Genome Coverage                   :: 7929.75x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:33:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,734
            CDSs (total)                      :: 3,639
            Genes (coding)                    :: 3,564
            CDSs (with protein)               :: 3,564
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 34 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077307.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1396"
                     /culture_collection="DSM:9312"
                     /db_xref="taxon:48296"
                     /plasmid="unnamed4"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            1087..3042
                     /locus_tag="I6L26_RS18550"
                     /old_locus_tag="I6L26_18550"
     CDS             1087..3042
                     /locus_tag="I6L26_RS18550"
                     /old_locus_tag="I6L26_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017389689.1"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="WP_004870594.1"
                     /translation="MKVKFLTVSLMFILPFSVFAANGQVEKAVIESNNAYKTKYNLDDM
                     TEFNYAQKGFIAKPTGQIKSEKGNVIWDFDAFNFLNDPAPNTVNPALWRQAKLNNNVGL
                     FKVTDRVWQIRGFDLANMTIIQGDTGWIIVDTLTSKETAEAALKFARQHLGEQKISAII
                     FTHSHIDHFGGALGVLSTEEINARKTPIIAPQGFMEEATSENLMAGSAMIRRATYMYGT
                     FLPKNAEGLVDTGLGKAVAVGKMGILEPTQLITQAEQKMTIDGLDFVFYNVPGSEAPAE
                     LTFSIPSLKLYNGAEILSHTMHNLYTLRGAKVRDALKWVGYLDQAMQHAKASDVLIAQH
                     HWPVWGNDNIQDFIKTQRDVYKFTHDQTVRYMNSGFNGAEIAEKIQLPAALDQKLYAHG
                     YYGTLKHNVKAIYQYYMGWFDAHPSNLDPLPPKAVAKKYIELAGGENNALKNARDAYAQ
                     ADYRWAAEILKHIVLNNPQNQQAKDLLANTYRQLGYAAEASTWRNFFLVGAQELQNNVP
                     LQNTSDPSDLLIHTPTERFLEAMATNLDVENLKNENQCINLVLSDTQENFSLWVENSIM
                     QFKRHDDSKDLASDCPTLTVTKPLYLKMITGQIKGVKVLLSNESKVKGNPLEIGKFFAM
                     FKRPDSTFPIVTRPND"
                     /name="alkyl sulfatase dimerization domain-containing
                     protein"
     gene            complement(3275..5089)
                     /locus_tag="I6L26_RS18555"
                     /old_locus_tag="I6L26_18555"
     CDS             complement(3275..5089)
                     /locus_tag="I6L26_RS18555"
                     /old_locus_tag="I6L26_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_004706791.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLSGDVDLTAQLID
                     SSPFAKKYTSGCLSFYEHALSDQDKQQIMQNFEECLFPGLDKDQYQILWVQHQDKVNQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNAEHQLYDPDDPNNQQL
                     LINKKNLPKDVKDFKDQLHQRIYQAVVDGKVADRQKLVEWLESNEIKVTRQTQKSISIE
                     NPYEGATRPIRLSGEVYEQGFSATGEYRQEVQQRIATYRGTTSERYRANVTDYQRQLEH
                     KSQYHSDRYPAVRREISAEPTEQLTADREAARPVPSLATVQIEPFKANERADTDKRTAS
                     SEQKPTETAYHFEYGNDFGSCYFNYSHYCARFYGQKQVQRDTDTQYDHQRETNASGSPE
                     PIGGQFDVQHMQTAKQQSPTSMYSDQQGRRGLAEQLHGADGVLSDDRIRNTVIENHRRT
                     TAAITATTNSVAAATAEFRRDAKENYPSLISTIKGNGERTDRLSANTKVIIDNTDTISR
                     IGKKYSDLYRADQWQSQGDEPNYNELRGRTGENLNHTTVSLVTSSQSSAEFARNVRTIE
                     ANIVQMKERKKEMEQPQPKRDRGMNFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5079..5480)
                     /locus_tag="I6L26_RS18560"
                     /old_locus_tag="I6L26_18560"
     CDS             complement(5079..5480)
                     /locus_tag="I6L26_RS18560"
                     /old_locus_tag="I6L26_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004706792.1"
                     /translation="MEIDSVTKQKVVRERTFKVRLSESEYQLLDANNPFGSIAKLLRQS
                     AVDKATKIYNSKADDAEKIKSTKIVESSYSKTERNLILELGRIGNNVNQIAKAINTDIA
                     GTGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(3279..5089)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctattaaacg aagtgaaata ggggtaactg tgtatccttg cttatttaaa aagggaaaga
       61 attatttata ctaaaattta atcaaatgat acagtatata aaccctaata agaactggca
      121 taaatcttgt aaaacaatag cactcaactc tttctcacca ccaccacagc cctcgctttg
      181 ctcaggcaga tggtgagcgt tgagagagtt tcggcaatac tctccctaag ggctgtcatt
      241 caaaaaataa cacatcccct aagtattgcc gatttaacct gattaggctg acggagccac
      301 gcatcggtcg tatccgcacc atgcttcaca tacatggcag acaaggacta aaggtactgc
      361 acctctcccc acgcgctccc tgtcgaagta tggcggtatc tctgcgagga tttctccaac
      421 atgcaactta accaacctat acaactactc tcaacgctcc gcttttgtgg gattcccctg
      481 taacggttca gctcctgctt ctcagctttc actatcgcta cgatgcacca gtgacactta
      541 ttccctccac atgcaccgca tatcttcctt aaaaaggatg cacagaagct gataaggctc
      601 gaatactgtc tatgtattgc ttattggatg aacgagagtc aggtaacgaa agacttttta
      661 ctgatacaat gcttgccatt ttttatcaat ttgatagaat ttgaaaagcg gtacaagccg
      721 aaaggtgaaa tcataacggt cgtcaaacct tatgatcttc gttacttgat gctcgaacat
      781 caaataactg cgtaccacca aatttaagac aaaacccctc taagttgcaa gactcagagg
      841 ggtttttctt taggttttaa caaaattttt atttttctct cctactaact catgcccaat
      901 aaggactgcg tagttttaat aaaaacaggt atattcttgt aaaatattat tttatgattc
      961 catcataaaa aatgatggct gttattataa tgtttgaatt gaaaagcttc tattaattca
     1021 acacatatca aaaatagtat agacaaggaa atcaacattt atggatattt tgaagaagga
     1081 aagacaatga aagttaaatt tttaacggtt tcattgatgt ttattttgcc attttctgtt
     1141 tttgcagcaa atggacaagt cgaaaaagcg gtcattgaat caaataatgc gtataaaacg
     1201 aaatataatt tagacgatat gacagagttt aattatgcgc aaaaaggctt tattgccaag
     1261 ccaacaggtc aaattaaaag tgaaaaaggc aatgtgatct gggattttga tgcgtttaat
     1321 tttttaaatg atccagcacc aaatactgtt aatcctgcac tatggcgaca agcaaagctc
     1381 aacaataatg ttggtctatt taaagtgaca gatcgagttt ggcaaattcg tggttttgat
     1441 ttggcaaata tgaccattat tcagggtgat actggctgga ttattgtgga tacattaacc
     1501 tctaaagaaa cggctgaagc agcacttaaa tttgctaggc agcatttagg tgagcaaaaa
     1561 atttcagcca ttatctttac ccatagccat atcgatcatt ttggtggtgc attaggcgtt
     1621 ctttctacag aagaaattaa cgctagaaaa accccaatta ttgcgccaca aggctttatg
     1681 gaagaagcga ccagtgaaaa tttgatggca ggttcagcca tgatacgtcg tgcgacctat
     1741 atgtatggaa cctttttacc aaagaatgct gaaggattgg tggatacagg tttaggtaag
     1801 gctgttgctg ttgggaaaat gggaatcctt gagcctacac aactgatcac ccaagcagaa
     1861 cagaaaatga ccattgatgg tttggatttt gtgttttata acgtgccggg ttcagaagcg
     1921 ccagcagagt taaccttctc tatcccatca ctgaaacttt ataatggcgc agaaattctt
     1981 tcgcatacca tgcataatct ttataccctc cgcggtgcca aagtccgtga tgccttaaaa
     2041 tgggtaggct atttagacca agcaatgcag catgccaaag catccgatgt attgattgca
     2101 caacatcatt ggcctgtctg gggcaatgac aatattcaag attttattaa aacccagcga
     2161 gatgtttata agttcacaca tgatcagacg gtgagatata tgaactctgg ttttaatggt
     2221 gctgaaattg ccgagaaaat tcagcttcca gcggcacttg atcaaaagct atatgcgcat
     2281 ggctattatg gaaccttaaa gcataatgta aaagcgatat accaatacta tatgggatgg
     2341 ttcgatgctc atccttccaa tttagatcca ttaccgccta aagctgttgc aaaaaaatat
     2401 attgagttgg caggtgggga gaataatgca ttaaaaaatg cgcgggatgc ctatgctcaa
     2461 gctgactata gatgggctgc cgagatttta aaacatattg tgttgaataa ccctcaaaac
     2521 caacaagcaa aagacttatt ggcaaatacg tatcgtcaat tgggctatgc tgctgaggct
     2581 tcaacttggc gtaatttctt tttggtgggt gctcaagagc tacaaaataa tgtaccccta
     2641 caaaatacat ctgatccgag tgatttattg attcacacgc caactgaaag atttcttgaa
     2701 gccatggcga caaatttaga tgtggaaaat ttaaaaaatg aaaaccagtg tatcaatctg
     2761 gttttgtcgg atacccaaga gaatttttca ttatgggttg aaaactcaat catgcagttt
     2821 aagcgtcatg atgacagtaa agatttggca tcggattgcc caacattaac cgtaaccaag
     2881 cccttatatc taaaaatgat tacaggtcaa ataaaagggg taaaagtcct gctttctaac
     2941 gaaagtaaag tcaaaggcaa tccattggaa attggaaaat tctttgcaat gtttaaacgt
     3001 ccagactcaa cttttccaat tgtcactcga ccaaatgatt aggatgaaat gactgggctt
     3061 aaagaaccct accaaattaa agtctccgct tttatgtgca cacattagtg cacataaata
     3121 caaaattagt ttaattattt caataattta caatcaataa aaaagcctaa cttgtctaag
     3181 tcgggctttt ttattgattc ttcacgtttc acgcctaata tttaatcatt gttccatatt
     3241 tttgattagc aacagtcgga atcgagcata aaccctacat gccaaaattc atgccccgat
     3301 ctcgcttagg ttggggctgc tccatttcct ttttccgttc cttcatttgg acgatattcg
     3361 cttcaatagt tcggacgttt cgagcaaatt ctgctgagct ttggctactt gtgacgagac
     3421 tgaccgttgt gtggttgaga ttttctccag tacgtcctcg taattcgttg taatttggct
     3481 catctccctg actttgccat tgatctgctc ggtataggtc gctgtatttt tttccaattc
     3541 gtgaaattgt gtctgtattg tcgataatga cttttgtgtt tgcactaagt ctgtctgtac
     3601 gctctccatt gcctttgatt gtgctgataa ggcttggata attttctttt gcatcacgtc
     3661 taaactctgc tgttgctgct gcaactgagt ttgttgttgc tgtaattgct gctgttgttc
     3721 ttcgatgatt ttcaataact gtgtttctaa ttcggtcatc acttaatacc ccatcggcac
     3781 catgaagttg ttctgccaat cccctgcgtc cttgttgatc tgaatacata gacgttgggc
     3841 tttgctgttt tgctgtctgc atgtgctgaa cgtcaaactg ccccccgatc ggttcaggtg
     3901 atccactcgc atttgtttct cgttgatggt catattgagt gtccgtatct cgttgtactt
     3961 gcttttgccc ataaaaccga gcgcaataat gagaataatt aaaatagcag ctcccaaaat
     4021 cattgccata ttcaaagtgg taagctgttt cagtaggctt ttgttctgaa cttgctgttc
     4081 ttttgtctgt atctgctcgt tcatttgctt taaacggctc aatttggact gtagcaaggc
     4141 ttggtactgg tcgagctgct tctcgatctg ctgtgagctg ttctgtaggt tctgcgctaa
     4201 tttctcgtct gactgctgga tagcggtcgc tgtgatattg gctcttgtgc tcaagctgtc
     4261 tttgataatc tgtgacgttt gctcgatacc gctcagaagt tgttcctcgg tacgttgcaa
     4321 ttcgctgttg tacttcttgt cgatattcgc cagtagcgct aaagccttgc tcataaacct
     4381 ctccacttaa acggatagga cgtgttgcgc cctcgtatgg attttcgatt gatattgatt
     4441 tttgggtttg tcgggtgact ttgatttcat tcgattcaag ccattcaacc aatttttgcc
     4501 gatcggcaac tttgccatcg actacagctt ggtaaatcct ttggtgtaac tgatctttaa
     4561 aatccttcac atccttgggt aagttcttct tattgattaa aagctgctga ttgttcggat
     4621 catcgggatc atatagttga tgttctgcat tggtaatctg cttaaacaga tccactcgat
     4681 ctaaatccac tggcgcataa aatggctgta gccgtttgcc tgtcgaaagc tcaacgtttg
     4741 gaatcacaaa attcagctca agccgtgtct ctccagtatc ttgattcacc ttatcttggt
     4801 gctgtaccca taaaatttgg tactgatctt tatcgagacc agggaaaagg cattcctcaa
     4861 aattttgcat gatctgctgt ttatcctggt cagataaagc atgctcgtaa aaggataaac
     4921 aaccactggt atatttcttg gcaaaaggac tgctatcaat cagttgagcg gtcaagtcaa
     4981 catcaccact taaaacctgt gcatgttctc gctctctatc ttcaccaagc aaataatcta
     5041 gacaaccttt agataatcct gaaccatgtc taaaaaaatc aacgatcatc tggtcgtaac
     5101 tcccgaagtt gttgatctat ggaaatcaac aaatgaagta acttcacttt gtcaaaagta
     5161 cctgtccctg caatatccgt attaatcgct ttggcaatct gattcacgtt attacctata
     5221 cgacccaatt ctaggattaa atttctctct gtcttgctat atgaagattc aacaatcttt
     5281 gttgatttaa ttttttcagc atcatctgct ttggaattat aaatctttgt tgctttatct
     5341 actgctgact gacgtagcaa ctttgcgatt gaaccaaaag gattattggc atcgagcaat
     5401 tgatattctg attctgaaag tctgacttta aacgtccgtt cccgtactac tttctgttta
     5461 gttactgaat ctatttccac ttgaacaatc ccattcttgg tctaaccggt ttagaggaaa
     5521 atctttgatt tttctttaaa ccggttttcg ggggtatcgg gggtgaagcc cctgatcaag
     5581 gtcacagctt agca
//
