LOCUS       NZ_CP077305            11469 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter pittii strain FDAARGOS 1396 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP077305
VERSION     NZ_CP077305.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357538
            Assembly: GCF_019048225.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11469)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 9.0.0, HGAP v. 4.0
            Genome Coverage                   :: 7929.75x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:33:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,734
            CDSs (total)                      :: 3,639
            Genes (coding)                    :: 3,564
            CDSs (with protein)               :: 3,564
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 34 of 75
            Pseudo Genes (incomplete)         :: 33 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077305.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11469
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS 1396"
                     /culture_collection="DSM:9312"
                     /db_xref="taxon:48296"
                     /plasmid="unnamed2"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            2149..3078
                     /gene="repM"
                     /locus_tag="I6L26_RS18440"
                     /old_locus_tag="I6L26_18440"
     CDS             2149..3078
                     /gene="repM"
                     /locus_tag="I6L26_RS18440"
                     /old_locus_tag="I6L26_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017401951.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005237331.1"
                     /translation="MSKELVVKTNRLNQAFQTLSLSEFHIVQLAIVDARHTGTGLSTDT
                     PLRIDALRYAEVFGTTRQNAYQRMKEAEDSLFNRRFSFFDEDGKLVKSRWIQQVKYLDD
                     EGAIELVFTLAVVQGISKIDGIKDFFTQYLLSQTAQLNSTYSARLYELLIQWRAIGKTP
                     VFELATFREQLGIGVNEYKRMDHFKTRVLDLAISEISEKTDIEATYQQHKKGRSISGFS
                     FSFKQKKSKTKSLENQTISGNLDLFSKKMTDSQRHLFSNKLSELPEMSKYSQGTESYPQ
                     FAVRIAEMLKDSEKLKEFAPMLEKVGYR"
                     /name="repM"
     gene            3147..3692
                     /locus_tag="I6L26_RS18445"
                     /old_locus_tag="I6L26_18445"
     CDS             3147..3692
                     /locus_tag="I6L26_RS18445"
                     /old_locus_tag="I6L26_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_171054282.1"
                     /translation="MKALSVIELAQLYGMNRQSIYKRINKGDLSKNSDGKIDFSEALRV
                     FGEPSHNNKDVTTLQSTSVQKETEVDMLKQQVDILKKQLELAHEREVFQREQLEAKDNQ
                     IEAIQRLLEAPKTNMTTFTDQKPSQDIATDPRSEPEPKHDGLTTPEQTENKRIPVPDHV
                     EPEQPKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(3889..>4023)
                     /locus_tag="I6L26_RS18450"
                     /old_locus_tag="I6L26_18450"
                     /pseudo=""
     CDS             complement(3889..>4023)
                     /locus_tag="I6L26_RS18450"
                     /old_locus_tag="I6L26_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004733157.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /name="cysteine hydrolase"
     gene            4142..4471
                     /locus_tag="I6L26_RS18780"
     CDS             4142..4471
                     /locus_tag="I6L26_RS18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005237346.1"
                     /translation="MKNLQIVIAQALHSYEFEHMEQSISKSISDTILEILVNDGPFEKI
                     DCYLYSKKDSKKILKSSIHYVIAVLERAKYRVQKAQQDNLSPLYICLYVIKKIEGFIEY
                     LNTKF"
                     /name="hypothetical protein"
     gene            4721..5008
                     /locus_tag="I6L26_RS18455"
                     /old_locus_tag="I6L26_18455"
     CDS             4721..5008
                     /locus_tag="I6L26_RS18455"
                     /old_locus_tag="I6L26_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_039890878.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDG
                     EERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERDKYEHG"
                     /name="BrnT family toxin"
     gene            4995..5309
                     /locus_tag="I6L26_RS18460"
                     /old_locus_tag="I6L26_18460"
     CDS             4995..5309
                     /locus_tag="I6L26_RS18460"
                     /old_locus_tag="I6L26_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_057095489.1"
                     /translation="MSMVKYSRKELNEKFSDKQDAEIERLLAKGTVSDDHLDLSDMPEI
                     TDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            5532..7670
                     /locus_tag="I6L26_RS18465"
                     /old_locus_tag="I6L26_18465"
     CDS             5532..7670
                     /locus_tag="I6L26_RS18465"
                     /old_locus_tag="I6L26_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="WP_057095490.1"
                     /translation="MIKVIWGTNKEKPIASRQLAETLSSNMSLEGFLYIGYPIVGSPLG
                     PTKFDALLLSESKGIIAFDLVEGTDLTNYINNQDEMASMLEVKLKPYPKLKKGRHLKFD
                     IKTVTFCPAKKNNLPQVEDNLYNIANISNLNEVINSFEWESDEDTFKELKAAIQVVTNI
                     RSGAIRRQTKKENSLGSRLQKLEDSIANLDQHQSEAVIETVDGVQRIRGLAGSGKTIVL
                     ALKVAYLHAQNQSWKIAVTFHTRALKEQFKRLINNFTIEQLGSEPDWDSIDIFNSWGAP
                     GDKENDGMYHRYCVENSIDYYDFQTAKNEFGGDDAFKNVCKLALEQSSSNKIEKYDLIL
                     IDEAQDFPPEFIKLCYKFLKEPKRLVYAYDELQSLNGLSVLPPEQLFGKDSKGVPLVTL
                     EEEDPTKPKKDIILEKCYRNSRPLLATAHTLGFGLTRPKGLIQFFDNDSLWEDVGYSVK
                     KGKIEGGKEVELIRTSESSPLFLEEHTPLEELIKFEVFDSVRDMNQMLFDSIVKNIHED
                     ELKPEDILVINPDPFTTQKNVGIVRKALQQNEIDSEIAGVTTSRDIFRKNGSVTFTGIF
                     RAKGNEAAMVYIIHAQDCADSYDPNHLALIRNRLFTAITRSKAWVRVLGIGPSMQALKE
                     EWETLKNNDYALKFVYPTEKQKNTLKLINKDMSVQERNRRRKLTHNIQEIIHAINSGEL
                     PTELIPEELINILKKSGH"
                     /name="ATP-binding domain-containing protein"
     gene            7672..8343
                     /locus_tag="I6L26_RS18470"
                     /old_locus_tag="I6L26_18470"
     CDS             7672..8343
                     /locus_tag="I6L26_RS18470"
                     /old_locus_tag="I6L26_18470"
                     /inference="COORDINATES: protein motif:HMM:NF021564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2290 domain-containing protein"
                     /protein_id="WP_057095488.1"
                     /translation="MDSDAISKQCTEIIDRLILSGLSIEQSYPSTRMNASGDYEIGFKK
                     GIEISQTLKNIPYEDIYKSVLDEKSYHIHLIDGGLVSFNYIFEKNDDVFFIKKHRLSFY
                     PSPSLPSFEDCPELYSNDEIFLECTKKNLVKFPIRFDFDPKNHVNVTHPASHVTFGQFL
                     NCRIPVSMPVTPRKFILFLLRNFYYSGYTKSKNIFDKKMVNVIPFHTITSEERKIGHFI
                     L"
                     /name="DUF2290 domain-containing protein"
     gene            complement(8464..9627)
                     /locus_tag="I6L26_RS18475"
                     /old_locus_tag="I6L26_18475"
     CDS             complement(8464..9627)
                     /locus_tag="I6L26_RS18475"
                     /old_locus_tag="I6L26_18475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994878.1"
                     /GO_function="GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]; GO:0004024 - alcohol dehydrogenase
                     activity, zinc-dependent [Evidence IEA]; GO:0008270 - zinc
                     ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="WP_005237355.1"
                     /translation="MRALTYHGARDVRVESVPDPVIQEPDDVILRVTATAICGSDLHLY
                     RGKIPATEDGDIFGHEFMGIVEEVGPEVTEVKKGDRVIIPFVIACGHCFFCEHELMAAC
                     ENTNTGRGAILNKKQIPPGAALFGFSHLYGGIPGGQAEYVRIPKGNVGPFKVPGSLPDE
                     KVLFLTDILPTAWQAVNNAQVGRGSSVAIYGAGPVGLLAAACARMLGAEQIFMVDHHPY
                     RLSFAHQTYGVIPVNFDEVDAAEFIIQNTTGYRGVDAVIDAVGFEAKGSMLETVMTNLK
                     LEGSSGSALRQCIAAVRRGGVVSVPGIYAGPIHGFLFGDAFDKGLTFKMGQTHVHNYLP
                     QLLEHIENGDLSPDLIITHRMKLEDAAEGYRIFDKKEEDCRKVILTP"
                     /name="zinc-dependent alcohol dehydrogenase"
     gene            complement(9871..10203)
                     /locus_tag="I6L26_RS18480"
                     /old_locus_tag="I6L26_18480"
     CDS             complement(9871..10203)
                     /locus_tag="I6L26_RS18480"
                     /old_locus_tag="I6L26_18480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032049750.1"
                     /translation="MPKPFLGSADDLAKFLDKHFRLGVKENGSSSFKFLNKGEAYSVLK
                     YVARDVINEEVERLKVEDPKAVARYNPGPSKIRLLDPLKPSTTGYIHIVLKRRYGSGNY
                     CIRPIR"
                     /name="hypothetical protein"
     gene            complement(10288..11283)
                     /locus_tag="I6L26_RS18485"
                     /old_locus_tag="I6L26_18485"
     CDS             complement(10288..11283)
                     /locus_tag="I6L26_RS18485"
                     /old_locus_tag="I6L26_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032049752.1"
                     /translation="MIHYSPMSRYTAQKIVDKVGHGAYFYSHFSVEGEDNLFFPKIDKL
                     IKKLTDKYHLDLTSRQRSYRLNTKKEPIADLIVQKRVNSTIFDFWLLITTPNTHKFNTQ
                     LSQINLKPRLSGQRVAEAENVVWNRENEQQEISVIQDYFRDQEKFKFVLQKPYLKLNFG
                     NGKYVELVRLSHSTKNSKKYASNRKKSEKNYTWTWRYDEPTVHLIEKKYKEIINDLISN
                     PNKSVGIGKWQQLNADLQHYTVFKGNRHQVGRLFTQAVGYHYKKGQSNLRNAEYYQPLT
                     LSYLPRQENYAEDFIQFVILRRLFEETGREFGKENVHEENYNQLINQYLI"
                     /name="hypothetical protein"
     Typing          2150..3078
                     /name="rep_cluster_1261"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgtgcaatt aagtggttcc taagtgacaa tatttacacc tgtttttacc tgctatatga
       61 tggaattatt cattgactga agcagtctga ccaactgcct gaccatttcg tttttacgat
      121 tttgagcagg cgtcagcctg cttaaagcca tgttttttta gatagcgcac gttcctctac
      181 tagccatttt gggatgaccg attgagcgta ctacctctga aggggggttc ggtacgatct
      241 gatgggcgct ggagtttact ctttcgatcc aatccaagct gtgatttttc tttatgactt
      301 ttcacggggt tgggcgacaa ggacacggga gcctataagc aatcgaaaga ttgtgaaatg
      361 gtcgttaggt tagatatagc ctagctagat ggttgtttac atcgaaaacc ctgttctagc
      421 aattaccgtg agaacctgac aggacgttaa ggttcgttaa taacggttat gtattgcggc
      481 tcatgcacgt tggaaacaac ggataggcgc agttcatagg cacaactgta ggcgtttgag
      541 gtaacagcga tgttatcggc tgcggagagg aaacttggaa gcagcggcac cactgagtga
      601 gatagtctca agcaaggtgg gtcaacggat agctcaagca gtatacatcg ggatgcatga
      661 aataattcca taattgcccg agatacgtgt agatcaagag ccatacgctc agattttttc
      721 tgatttgcca tatccccatt tttttaagga accgcgttgg ggggaaactg gaagtgcagg
      781 tcaggagaga gagcattggc ggtctggtcg ttaagattgc tagaaataac ttgggaatct
      841 cttagctgct gctgtcatgg cactggtagg ttaagagagg gagagacgat gagtagggcg
      901 tataaggcat ataacttgtt atatgttgaa gtcgtggcaa atattgctaa aacaataggc
      961 gaactcaatt gcgactggca gtcgtaattg gtaaagggag tctaaactct atgtgatgtt
     1021 tacgtggagc cgtttccgaa agtttgagtc ggttttatgt tgttatctaa aaaagcgaaa
     1081 aactcgttaa agatggtttg gtcgccatcg ttgttttgct tgggtcagga ttggtctcct
     1141 gacttatgca agactaaaca acagtctaat gaaagaatgt gatgtgatat ttagccactt
     1201 ttagagtgca acagtcttgt taatcaccgc tctgttcaat agtgccaggt gctatttctc
     1261 tggatagttt taaagtttag aaaaactgat gttagcagac attccaatct gtgagttttt
     1321 actctttttt agtaaagaaa gggcgtactg tgatgattct taggctctgc cgacctaagt
     1381 tattcgataa acagcgtggt agttacgtca gtgcactata gccccctcgg ttttcttcgg
     1441 caaagaaagc gaaacgaggt gggtgtcttt aatttctaaa atgcgatatt ttgcgatttt
     1501 aggagctttc taacttagga catagaatct atgcttaaac gtagtaaatg agctaaaacc
     1561 cccatttttt gcgtttaaaa gccattctca gaacgagtgt ctacgagtga acattgaaaa
     1621 gttgcgccta gacttcctga aaaacagttt tttcagtata taaatttcta aaaacagaaa
     1681 attatgtctc caaagtctac ttcaaatcga gcgtagcgag caaaaaaagc tcatgagcga
     1741 agcgaattcc gagttgcttt tgctttttct aaaattcacg aaaatattaa ctaaaaaatt
     1801 gccccgacga atcgagcgag agcgagattc aatagagttt gagcgtagcg aaaaccaagg
     1861 gcaatttttc attccctgtg tttttaatta ttttaaggtt tttaaatgtt tttaacttat
     1921 ttttaattat taaatttcaa taactaatct ttgtattccc ccacgtttag ttacccattc
     1981 ccccacgttt agttacccat tcccccacgt ttagttaccc attcccccac gtttagttac
     2041 ccattccccc acgtttagtt accctcctaa ttacaccctt aactacacag ttgtagttat
     2101 acccacattg ttataatctg tgggtataaa aatatttcaa tcgagtctat gtcaaaagaa
     2161 ttagttgtaa aaaccaatcg gttaaaccaa gcttttcaaa ctctatcttt atcagagttt
     2221 catattgttc aattagccat tgttgatgct agacatacgg gtactggact aagtacagat
     2281 actcctttac gaatagatgc tttgagatat gctgaagttt ttgggacaac aaggcaaaat
     2341 gcttatcaga gaatgaaaga agcagaggat tctttattta acagaagatt tagcttcttt
     2401 gatgaagatg ggaaattagt caaaagtaga tggattcagc aagtaaaata tctagatgat
     2461 gaaggagcta ttgaacttgt tttcacttta gctgttgtac aaggcataag taaaattgat
     2521 ggtattaaag acttttttac tcaatattta ctaagccaaa cagcccagct taacagtaca
     2581 tattcagcaa gattatacga gttattaatt caatggagag ctattggaaa aacgccagta
     2641 tttgaattag caacttttag agaacaactt ggaattggtg ttaatgaata caagcgtatg
     2701 gatcacttta aaactagagt tcttgactta gccatttctg aaattagcga aaaaacagat
     2761 atagaggcaa cctaccaaca gcataaaaaa ggccgttcaa tatctggttt ttctttttct
     2821 tttaaacaaa aaaaatctaa aactaaatca ttagaaaatc agacaatatc tgggaatcta
     2881 gatctttttt caaaaaaaat gactgactct caacgccact tattttccaa taagctatct
     2941 gaacttcctg aaatgagtaa atactctcaa ggaactgaaa gctatcctca attcgcagta
     3001 agaattgctg aaatgcttaa agattctgag aagttaaaag agtttgctcc aatgcttgaa
     3061 aaggttggct atcgttaaat cattgatgta acgttacatc actaaatact gtctactgac
     3121 atacagactt ttacttgggt tacatgatga aagcactatc tgttattgaa ttagctcaac
     3181 tctatggaat gaatcgtcaa agcatctata aacgtataaa taaaggagat ttatcaaaaa
     3241 atagtgatgg aaaaatagat ttttcagaag ctctacgtgt atttggtgaa ccatcccaca
     3301 ataataagga tgtaaccacg ttacagtcta cttcggtaca aaaagagaca gaagtagaca
     3361 tgcttaaaca acaagtagac atactaaaaa aacagttaga acttgcacac gaacgagaag
     3421 tttttcaacg agaacaacta gaagcaaaag acaatcaaat agaagcaata caacgactgt
     3481 tagaagctcc aaaaaccaat atgactacgt ttaccgatca gaaacctagc caagatatag
     3541 caacggatcc tcggtcagaa cctgagccga aacatgacgg attgactact ccggaacaaa
     3601 cagaaaataa gcggattccg gtgccggatc acgttgaacc ggagcagccg aaaagaggct
     3661 tttggagccg tttttttaga ccctatgatt aaccacgatt gtttaactaa tatgaataaa
     3721 gtcgtgaaat attgatcaaa taataggcgt ggaacgtgaa aaatcataaa aaaaaaagcc
     3781 caccttgggg aggttgggca aaaaagaaac tacagcgttg aattttcaag agaattataa
     3841 cgcatttcgt ataaggtgta ttatgttaat tttaggaaat cttaagcttt agccaacata
     3901 atcttcagtt gatattactt gagcatatgc aaattcaaaa gcggccatta atgttgcatg
     3961 gacatgacca gcaggtactt taacgccatt aaattccaag tcagctctgg ccctagaatc
     4021 atttttttaa cttttcataa cgaagaataa aaataactca atgtttactt taggttttat
     4081 acgctttttc ttttcaggag ataatgctag gatcaaaatt ggctaacatt aaataatcat
     4141 aatgaagaat cttcaaatag ttatagcaca agctctacat tcatatgaat ttgaacatat
     4201 ggagcaatca ataagtaaat ccattagcga cactatattg gaaatattgg tcaatgatgg
     4261 gccttttgaa aaaattgatt gttatctgta ttcaaaaaaa gattctaaaa aaattctaaa
     4321 aagctctata cactatgtaa ttgctgtctt agaaagggcg aagtatagag tacagaaagc
     4381 tcagcaagat aatctgagtc ctttgtatat ctgcttatac gtgattaaga aaattgaggg
     4441 ctttatcgaa tacttaaata ctaaatttta actgtgaaag cctccattta acataatggc
     4501 gattatacga agtacacttg tatattaata taaatatcag atacttaaaa ttttggctct
     4561 agccaaaaaa tgcacaaaaa gccgattttg aaacaagtcg gcttttttat gagcagtttt
     4621 gaaatatttt gccaataaat ttgactaaaa attacgcttt tcagcttgag tgatacaatt
     4681 ttgcgataca ttattgtata tacaaatttg aatgtttttt atggaacagt attttgaatg
     4741 ggatgaggct aagaatcgaa agaatcagaa aaagcacgat gtctctttcg aaactgcaag
     4801 ccttgttttt gaagatccgt tgaggatctc aatccaagac agacataccg atggcgaaga
     4861 acgttggcag accattggga aagtaaaagg cgtactaatg ctcttagtag ctcacaccat
     4921 ctttgatgaa gatgactgtg aaatcatacg aatcattagt gcaagacaag taactaaagc
     4981 agagcgagat aaatatgagc atggttaaat actcacgcaa agaactgaat gaaaaattca
     5041 gcgacaagca agatgctgaa attgaacgct tgcttgctaa gggaacagtt tctgacgatc
     5101 atctagatct atcagatatg cctgaaatta ccgactggag taatgctgta cgccacaatc
     5161 aattctatcg tccagtgaag caacaaacat ctattcgttt agatgctgac gtacttgcat
     5221 ggttcaaagc acaaggcaaa ggttatcaga cacgtatgaa tgaaatctta cgggatgcta
     5281 tgctaaaaga attaaaaaat catcaataaa aatgggagct taggctccca tttttgactg
     5341 cgtataacgt gtattatatt aattttagat atttttaata ataagcaatc tctaaattga
     5401 gatgtttaag tttatttgat ttcgaactaa ccataagagt tattcatatc aatgcttaac
     5461 ttgataagac taaggcttat aaatatagta gagcattgct tgatttcaag attttaaata
     5521 aaggtattgc tgtgattaaa gttatttggg gcactaataa agaaaaacca attgcttcaa
     5581 gacaacttgc ggaaacatta tcttctaaca tgagtttaga aggctttctt tatataggtt
     5641 atccaattgt aggctctcct ctaggaccga caaaatttga tgcactactt ttaagcgagt
     5701 caaaagggat cattgctttt gatttagtgg aaggaacgga ccttacaaac tatataaata
     5761 atcaagatga aatggcctcc atgttggagg tgaaattaaa accttatccc aagttaaaaa
     5821 aaggtcgtca tcttaagttt gatattaaaa ccgtaacttt ctgtcccgct aaaaaaaata
     5881 atttgccaca agtcgaggat aatctataca acatcgctaa tatttccaat ctaaatgaag
     5941 ttatcaattc atttgagtgg gaatcagatg aagatacgtt taaagaatta aaagcagcaa
     6001 ttcaagttgt tactaatatt cgatctggtg ctattagaag acaaactaaa aaggaaaatt
     6061 ctttaggaag tcgcctacaa aaattagaag attctatagc gaacctagat cagcaccagt
     6121 ctgaagcagt cattgaaaca gtggatggtg tacaacggat cagagggtta gcaggatcag
     6181 gaaaaactat cgtattagcg ttaaaagttg cttatttaca tgcacagaac caaagttgga
     6241 aaatagcagt aactttccat acacgtgcgt taaaagaaca gtttaaaagg ctaatcaaca
     6301 actttactat tgaacaatta ggtagcgaac ctgattggga ttctattgat atatttaact
     6361 catggggagc acccggagat aaagaaaacg atgggatgta tcaccgatat tgtgttgaaa
     6421 actcaataga ttactatgat ttccaaacgg ctaaaaatga gttcggaggg gatgatgctt
     6481 tcaaaaatgt ttgtaaacta gctttagaac agtcctcatc taataaaata gaaaaatatg
     6541 atttaatttt gattgatgaa gctcaagact tccctcctga gttcattaag ttatgttata
     6601 aatttttaaa ggaacctaag cgattagttt atgcatatga tgaacttcag tctttaaatg
     6661 gtctgtccgt tttacctcct gaacagttat ttgggaaaga ttccaaagga gtgccattag
     6721 taaccttaga agaagaagat ccaacaaaac ctaaaaaaga tattattctc gaaaaatgtt
     6781 accgcaattc acgtccacta cttgcaaccg ctcatacact agggtttgga ttaactagac
     6841 caaaaggatt aattcaattt tttgataatg attctctatg ggaagatgtt ggttattcgg
     6901 ttaaaaaagg taaaattgaa ggtggaaaag aagtagagtt aattagaact agtgaatcaa
     6961 gccctctatt tttggaagaa catacacctc ttgaagaact tataaaattt gaggtatttg
     7021 attcagttcg agatatgaat caaatgcttt tcgattctat tgtcaaaaat atccatgaag
     7081 atgagcttaa gcctgaagat atcttagtta ttaatcctga ccctttcaca acacagaaaa
     7141 acgtagggat tgtaagaaaa gcattacaac aaaatgagat agattctgaa attgcaggtg
     7201 tcacaacctc cagagatatc ttcagaaaaa atggatctgt tacttttaca ggtatcttca
     7261 gagcaaaagg taatgaggct gcaatggtct acattatcca cgctcaagat tgtgcagatt
     7321 cctatgatcc aaatcactta gcactaatca gaaatagatt atttactgct attactcgga
     7381 gtaaagcttg ggttcgtgtc ttgggaattg ggccatctat gcaggctcta aaagaagaat
     7441 gggaaactct aaaaaacaac gattatgctt taaagtttgt ttatccgaca gaaaagcaga
     7501 aaaatacatt aaagttaatt aataaagata tgtctgttca agagaggaat agacgaagaa
     7561 aactgacaca taatattcaa gaaattatac atgctatcaa ttcaggagaa ttaccaactg
     7621 aattaattcc tgaagaatta attaatattt taaaaaaatc agggcattaa gatggattct
     7681 gatgctattt ctaaacaatg tactgaaatc attgaccgcc ttattttatc tgggttatca
     7741 atagaacaaa gctatccatc cacacgtatg aatgcgagtg gagattatga aattggattt
     7801 aaaaaaggta ttgaaatttc tcaaacgcta aaaaatatcc cttatgaaga tatatataag
     7861 tctgttttag atgaaaaaag ctatcatatt cacctgatag atggaggctt agtttctttt
     7921 aattatattt ttgagaaaaa tgatgatgtt ttttttataa aaaaacatag attgtcattc
     7981 tatccttcac catcattgcc atcctttgaa gattgcccag aactttatag taatgatgaa
     8041 attttcttag agtgtactaa aaaaaattta gttaaatttc ccattagatt cgactttgat
     8101 cccaaaaatc atgtgaatgt tactcatcct gcatcgcatg taacatttgg gcaatttctc
     8161 aattgtagaa tacctgtttc aatgccagtt actccaagaa aatttattct atttttactt
     8221 cgtaattttt attattctgg ctatacaaaa agtaagaata tttttgataa aaaaatggtt
     8281 aatgtcattc catttcatac tattactagt gaagagagaa aaattggtca cttcatttta
     8341 tagttcacct atactttatt taacataaaa tctcttatat gcaattcagt tgtaagccca
     8401 aatggaaatg tccgatttct ccaaagtaaa aaagcgcctg atggcgcttt cctttaatca
     8461 tatttatggc gtcagaataa ctttacgaca gtcttcttct tttttatcaa aaatacggta
     8521 accttccgca gcatcttcca gtttcatgcg atgggtaata atcagatctg gagacagatc
     8581 tccattttca atatgttcta gcaactgcgg caagtaatta tgcacatgag tttgtcccat
     8641 tttaaaggtt aaacctttgt caaaggcatc accaaacagg aagccgtgaa ttggaccggc
     8701 ataaattcca ggtacactca ccacaccgcc gcggcggacc gctgcaatac attgtcttaa
     8761 cgctgaacca ctcgaacctt ctaatttcag gttagtcatt acggtttcca gcatgctgcc
     8821 tttggcttca aaaccgaccg catcaatgac ggcatcgact ccacgataac ccgtggtatt
     8881 ttgaatgata aattcagcgg catcgacttc atcaaaattg accggaatca ccccataggt
     8941 ttgatgtgcg aaactcagac gataaggatg gtgatcgacc ataaaaatct gctcggcacc
     9001 gagcatccgc gcacaggcag cagccagcag accaactggg ccagcaccat aaatcgccac
     9061 actggaacct cgaccaactt gggcattgtt cactgcttgc caggcggttg gtagaatatc
     9121 ggtcaggaat aatacttttt catcaggcag ggagcctggc actttaaatg gtccgacatt
     9181 gcccttgggg atccgcacat attcagcttg accacccggt ataccaccat atagatggct
     9241 aaagccaaat aaagctgccc ctggtggaat ctgtttttta ttgagaattg caccacggcc
     9301 ggtattggta ttttcacagg cagccatgag ttcatgttca cagaaaaaac agtgaccgca
     9361 tgcaatcaca aagggaataa tcacccggtc accttttttg acttcggtta cttcagggcc
     9421 gacttcttct acaattccca taaattcatg gccgaaaata tcaccgtctt cagtggccgg
     9481 gattttcccc cggtataaat gcagatctga gccacagatg gcggtggctg tcacccttaa
     9541 aataacgtca tctggttcct gaatcactgg atctggaacg gattcaactc gtacatcccg
     9601 agcaccatga taggtaagag cacgcatgcg gatttcctct ttctagttca acattaaaca
     9661 gactcgtatc aaggctgcca aaaataaaaa acacttttat tagagactta tgcgccaaga
     9721 aatcgctgta aattaatgta tgaaataaga catccgagtg tttaaaaaat aaatttatat
     9781 agggtttacg taattcgtga tcttatgtgt cgaatgaata gaaaagaaca gaattaaaga
     9841 aacacctaga ttgttagaac ccatttctag ctatcttata ggcctaatgc agtagtttcc
     9901 gctgccatat cttcgcttaa gtactatatg tatataccct gttgtagaag gtttaagagg
     9961 atccaataat ctaattttag aaggtccggg attgtaacga gctactgctt tcggatcttc
    10021 aacttttaaa cgctctactt cttcattgat aacatcacgg gccacatatt ttaaaacgct
    10081 ataagcctct cctttattca gaaatttgaa agaggaactt ccattttcct ttactcctaa
    10141 acggaagtgc ttatctaaaa atttagctaa atcatcagca gaacctaaaa aaggttttgg
    10201 cataatcttc tccttaaact aatccttata ttattcagca ttagaactgc aaactttatt
    10261 aagtatcaat ccattaacag ttttaattta aataagatat tgattaatca attgattata
    10321 attttcttca tgcacatttt ccttaccaaa ctctcgtcct gtttcttcaa aaagacgtct
    10381 taaaatcacg aattgaataa aatcttccgc ataattttct tgtctaggca aatacgataa
    10441 cgtcaacggc tgataatatt cagcgttcct caaattcgat tgtcctttct tgtagtgata
    10501 gccgacagct tgcgtgaaca gtcgcccgac ttgatgacga ttacctttga acacagtgta
    10561 atgttgtaaa tcggcattta actgctgcca tttcccgatg cctactgatt tattagggtt
    10621 agaaatcaaa tcattaataa tttctttgta tttcttctcg attaaatgaa cggttggttc
    10681 gtcataccgc catgtccaag tgtagttttt ctcactcttt ttacgatttg aagcatattt
    10741 ttttgagttt ttggtcgagt gtgaaagtcg aactagctcg acatacttac cattgccaaa
    10801 attgagcttt aaatagggtt tttgaagtac aaatttgaac ttttcctgat ctcgaaagta
    10861 gtcctggatg acgcttattt cttgttgttc attctcacga ttccaaacaa cattttcagc
    10921 ctcagctact cgttgtccag atagacgtgg tttcaagttt atttggctga gctgcgtgtt
    10981 aaatttatga gtattaggcg tagtaataag aagccaaaaa tcaaaaatcg tactgtttac
    11041 acgtttttgc acaatgagat cggcaatagg ctctttttta gtgttcagtc ggtatgatct
    11101 ctgcctcgaa gttaggtcta agtggtattt atcggttaat ttcttaatca gcttatctat
    11161 tttagggaaa aaaaggttgt cttctccctc aacagaaaaa tgagaataaa aataagcgcc
    11221 atgcccgacc ttatctacga ttttctgagc tgtataacga ctcattgggc tgtagtgaat
    11281 cattttattg tcaccaagca atatttataa aattgtcatc aaacaatatt acaaaaactt
    11341 gattgtaatc aagcaatatt tataaaaagt taaagactta ttttataaga tttttataaa
    11401 aatttagtgg cgaagcgact tttgccaatg ccgaaggcat tggccggact taattgacac
    11461 aatttgtca
//
