LOCUS       NZ_CP076868             9294 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain V3 plasmid unnamed5, complete sequence.
ACCESSION   NZ_CP076868
VERSION     NZ_CP076868.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19730657
            Assembly: GCF_019038575.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Janssen,L., Almeida,F., Campos,T., Pappas,G., Martins,V. and
            Damasceno,T.
  TITLE     A novel multidrug resistant, non-Tn4401 genetic element
            (NTEKPC)-bearing, strain type of Klebsiella pneumoniae isolated from
            an urban lake with drinking and recreational water reuse
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Janssen,L. and Almeida,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUN-2021) Department of Cell Biology, University of
            Brasilia, Campus Universitario Darcy Ribeiro, Instituto de Ciencias
            Biologicas, Bloco K, 1 andar, Brasilia, DF 70910-900, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7-b1585
            Assembly Name                     :: KpV3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 470.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:23:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,343
            CDSs (total)                      :: 5,223
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 84 of 216
            Pseudo Genes (incomplete)         :: 57 of 216
            Pseudo Genes (internal stop)      :: 102 of 216
            Pseudo Genes (multiple problems)  :: 20 of 216
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076868.1.
            Bacteria and source DNA available from Laboratorio de Analises
            Moleculares de Patogenos, Block I, Institute of Biological
            Sciences, University of Brasilia, Brasilia, Brazil.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="V3"
                     /isolation_source="lake"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Brazil: Paranoa Lake, Brasilia"
                     /lat_lon="15.740962 S 47.881235 W"
                     /collection_date="2019-02"
                     /collected_by="Luis Janssen"
     gene            1020..2408
                     /locus_tag="KRP25_RS26435"
                     /old_locus_tag="KRP25_26435"
     CDS             1020..2408
                     /locus_tag="KRP25_RS26435"
                     /old_locus_tag="KRP25_26435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_228744105.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIARCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTRRKTNIQILGLPGSGKSVIATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNSGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRE"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            2424..2984
                     /locus_tag="KRP25_RS26855"
     CDS             2424..2984
                     /locus_tag="KRP25_RS26855"
                     /inference="COORDINATES: protein motif:HMM:NF024108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_043019015.1"
                     /translation="MLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLP
                     AEFVKTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDS
                     FIQKEARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDP
                     LAEFRPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            2984..3715
                     /locus_tag="KRP25_RS26440"
                     /old_locus_tag="KRP25_26440"
     CDS             2984..3715
                     /locus_tag="KRP25_RS26440"
                     /old_locus_tag="KRP25_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            3722..4252
                     /locus_tag="KRP25_RS26445"
                     /old_locus_tag="KRP25_26445"
     CDS             3722..4252
                     /locus_tag="KRP25_RS26445"
                     /old_locus_tag="KRP25_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_217049281.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLARQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4280..4459)
                     /locus_tag="KRP25_RS26450"
                     /old_locus_tag="KRP25_26450"
     CDS             complement(4280..4459)
                     /locus_tag="KRP25_RS26450"
                     /old_locus_tag="KRP25_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4528..4905
                     /locus_tag="KRP25_RS26455"
                     /old_locus_tag="KRP25_26455"
     CDS             4528..4905
                     /locus_tag="KRP25_RS26455"
                     /old_locus_tag="KRP25_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_217049282.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWQWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(4967..5266)
                     /locus_tag="KRP25_RS26460"
                     /old_locus_tag="KRP25_26460"
     CDS             complement(4967..5266)
                     /locus_tag="KRP25_RS26460"
                     /old_locus_tag="KRP25_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5256..5519)
                     /locus_tag="KRP25_RS26465"
                     /old_locus_tag="KRP25_26465"
     CDS             complement(5256..5519)
                     /locus_tag="KRP25_RS26465"
                     /old_locus_tag="KRP25_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(5825..6244)
                     /locus_tag="KRP25_RS26470"
                     /old_locus_tag="KRP25_26470"
     CDS             complement(5825..6244)
                     /locus_tag="KRP25_RS26470"
                     /old_locus_tag="KRP25_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            6647..8332
                     /locus_tag="KRP25_RS26475"
                     /old_locus_tag="KRP25_26475"
     CDS             6647..8332
                     /locus_tag="KRP25_RS26475"
                     /old_locus_tag="KRP25_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8342..8599
                     /locus_tag="KRP25_RS26480"
                     /old_locus_tag="KRP25_26480"
     CDS             8342..8599
                     /locus_tag="KRP25_RS26480"
                     /old_locus_tag="KRP25_26480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            8684..8833
                     /locus_tag="KRP25_RS26485"
                     /old_locus_tag="KRP25_26485"
     CDS             8684..8833
                     /locus_tag="KRP25_RS26485"
                     /old_locus_tag="KRP25_26485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1647..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4..221)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.455"
                     /coverage="99.0"
                     /evalue="3.52e-75"
     Typing          complement(8937..9294)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.611"
                     /coverage="99.0"
                     /evalue="1.09e-179"
     Typing          2985..3712
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 ggaaacggca gggttggaac aggagagcgc aagagggagc gattcatcgg aaacggtgat
       61 gatctttaag tcctgtcggg tttcgccact cctgactgat tcatggttga gccaccggct
      121 cccacagatg caccgaaaaa gcgtctgttt atgtgaactc agtcaggagg gcggagccta
      181 tggaaaaacg ccacctgcgt ggccttgccg ttttgcctca catgttagtc ccctgcttat
      241 tcacggaatc tgtgggtaac tttgcatgtg tccgcagcgc ccgccgcagt ctcacggccg
      301 gagcgtagcg accgagtgag cgaggaggcg caatttcccc ggcacctaca agacggacac
      361 cggacgcgct gatgtggcat attgtcccct gattaagtct ttatattccc tgataataaa
      421 ttaccgggat aggtgaatca tatcggtttt tcgctgaaca ttttacgtta acctatctct
      481 gttgcttttt tctccttacg gagaaataca ggtcatcccc gcgagggcgt ggctgacctc
      541 agggggatat cccctgctta cgctggctgg acgccaccga cgcacggtct ctccccctct
      601 ttctggctcc gtccggctgg acgccggcca cgccagaaat tcacccggcc cccgtcaggc
      661 tcacgcctga ccacccacaa aaatccacgg tgctggacgc acctggcgaa gcagcaaagc
      721 tgctcctgat tttgcgggtg gtcagccgct cgctatggcg aactctgacg ggggccggaa
      781 caccggaaaa gaccgcactg gggtgccgcc ttttcctctc acacgctggg gcgtgctcgt
      841 tgttcctccg gggctgcgcc ttttccgcat ggggcggaaa gaggctggcc tccgtggtga
      901 aagctgaacg caatcaccac ggacgtgacc gccgccgcca tgccttgggg catgtccgcg
      961 gcttgtgcag cgggggctgc acatcacgcc ggcaaaccat cgtctgaagg agagagaaca
     1021 tgtttaatac ggattcgctt gcctggaagt ggcacattcc ggagctggcc cggcagattt
     1081 atccgtgggt cagctggctg taccaggagt attacggtct ggccggggct ttcctagtta
     1141 tcggcctgtt tttcggtctg gctgaacttt gggccggacg gatgcgtttt gtggggctga
     1201 gtcataagga gcgacgggag tatgccgatc gctataagcg cgataataaa cggccagcgc
     1261 cattttcggt gctggcctcg ctggattcgc ccgggcggat ggtcagggca cgtcgacatg
     1321 tctggaacta cgcgtactgg ctgaccggcg cggccggtct ggcccttctg ggggggctgg
     1381 ttgtcgccgt gtttatcgag agctggggag aaaccgtcct gcgtccggac ctctacgcaa
     1441 acgcagatta ttacctgctg cagccggcta acctgttcgg gattgcccgg tgcgcgggcg
     1501 ggctggctgt cgggattgtg ttggcgcggc tgctggcgtt cctgctgctt gacggtcatt
     1561 ttgcggctgc tgacgaggcg gtgaacgcga aactgaatca ggagagccgg cgcagcagcc
     1621 agcggacagg agagatgacc gacgtgcgcc acctgcattt cggggagccg gtaccggtta
     1681 atgcgctggc cgatttttcc tcggagcagg cccggcagga gcaggcggtg tttctgggta
     1741 aggatgagca gggcaagccg gtgctggttc cgcgtgatac ccggcgcaag accaacattc
     1801 agatcctcgg cctgcccggg agcggcaaga gcgtgatagc caccaacgcg ctcattcgct
     1861 gcgtacgcga tttcggggac gcggtggtgt acttcgaccc caaaggggac gcatgggcgc
     1921 cgcacgtctt ccgggcgcac tgtccggact tcaccctgct ggatctgcgg ccggggaaac
     1981 cggcgcagct gaatctgttc cgcgatctcg accagtacgc gctgaaaaac ctgctgattg
     2041 ccgggcttaa ctcgggcgag accggcgacg tggcggacca ctaccgcatc agcgagcaga
     2101 aagcggcgaa gctgattgcg gagcagtttc cgcagggggc aaacattcag caggtgctgg
     2161 cggcggcgta cgcgctgccg gaggccctga aaaaggacgt gaaggggttg atcactaggc
     2221 tggaaaacgt cgctgacctg agcgtcctgc agacggacag tggcatcgat gtggcgggga
     2281 ttatcaacgg cggcggctgc ctgtacgtta tcggctcgat ggacgacgag gcggtgatcc
     2341 gtgtgcaaaa aatgctgttt gcccgctgcg cgcaaatcat cattgcgcgg gacgagttcc
     2401 gggagtagct acacgtcagc gtgatgctcg atgaaattaa atatctgctg tcgaagtatg
     2461 tactgaacgc actggggacg ctgcgcagcc gggactgcaa tctgttgctc gcgcaccagt
     2521 cgctgggcga tttcgggcag tgcgggcagg atctgcccgc tgaatttgtg aaaaccacgg
     2581 tactggataa cacgccgatc cgctggtttt accgggcggc cagcctggag tccgcgcagt
     2641 gggcggcggg gcagaccggg gaaatcaggg tggacgtgga gcgccgccgt gccagccggg
     2701 aggcggggaa cgtggagcat atcagcgggg acagcttcat ccagaaggag gcgcggccgc
     2761 tgtttgacgt gaacaccctg caacacctgc cggacgggtt cgcggtcatg accgggctgg
     2821 gtgtggcccg gatggcgttc agcagtccgc tgcgggttga gcgccgggaa atcccgttgc
     2881 agcgtttccc ggtgctggcg aaaaccgatc ccctggcgga gttccggccc ccggccgacg
     2941 gcggcgggcc gaaagcggat gatgattttg cggggattta ctgatggcgc tggagctgta
     3001 caacgtcagc cacgcgaaac gacaggcgcg gaacgcggag aaaacccggc tgaccctgcg
     3061 ctggttgcgg gaagagctgt gttccacggc ggagctggtt gcgcggcggc tggggattgc
     3121 ggcggtgcag ccggtctacc gcttcctcga cagcctggtg gcgaaagggc tgctggtccg
     3181 gaccaaatat ccggtggacg gccgtcaggt cagcgtgtgg gggctgacgc cgcatggcgt
     3241 ggcgttctcg ttcgacgagg acgaaccgct ggcggatgtt attccgtttc agccgtcaag
     3301 ggtgtcggca gcacatctgc cgcaccggct ggccgtgcaa tccctgcgcc ttgatatgga
     3361 agccagaggg gccaccggct ggcgcattct gcaccgtctg agtcttaaag gcatgaaagt
     3421 gccggacgcc ctggcggagc tggacggtaa aacggtggcg tttgaggtgg agcgcaccgt
     3481 caagagccgt cgccggtatc aggaagtggt gtccgggtat ctgttcaacc gcaaagctaa
     3541 cggcatcgat gaaatctggt acatctgccc ggaccgggcg acgcaggtca gggtgcagcg
     3601 ggcaatcctg tcggtggatg aaatcgtgaa cccacagacc ggcgaggccc gcaaaacggc
     3661 agagctggat cgtgaacggt tgtttgcgtg ctttaaattt atgacaacgg agtgagggat
     3721 tatggcgctg tacatggtat ttattgaaga cgcggaggac cggaacggcg agcgctgcat
     3781 catgccgacc agcgagcatc tggaagatat ggtggtgctc gcccggcaga ccggggcgta
     3841 tgttttctgt tttgtcgacc gggcccagca tctggcgatg atgaaatcca tcgggatgta
     3901 tgacgagcgg ctcaacagcg acgaaaaagc cacgtacttt ttcactggta aaaatccgca
     3961 atgggaacgg aacaaaccca caggaatgaa gctgtgtgat tacctctatc gcaaggtgga
     4021 gcggttcgcc atcgagaacg gtgtgcaggt ggttgatgcc gggaagtgct caccggagca
     4081 gattaacgcg atgattgacg tggtgagtga gcggcacggg gaaagcgcgg tgaacgagaa
     4141 gctgaaagcg gagctgatgc gggaatataa cagccgctat tttaacgtgc cggtacctga
     4201 actgccggag gacaaacctg cccgccggtg gtggccgttc ggcaaaaaat aaatgccagc
     4261 accggccatg ccggtgctgt tattcctccc cgagcttatc ccgcatcacc tgccagagtg
     4321 tttccgcatc ctcgtgtagc cgctcgcaca tgtcggccgg ggtatccata tccagctcat
     4381 tcagtttatc gagcaatagc agcgactgct ccttaataaa tttagccata ttgagggctg
     4441 ttttttcctg ttttgtcatg attctttcct tccgggcatg gtgtgcgcct gtcaggtata
     4501 ctgtctcaaa aatagcgggg tgagacaatg gcgtgggtga cggtgagaca ggcgacggaa
     4561 ctgacgggta aagcccgaag ttccctgtat cgggatatgg cgaaaggccg cgtatcatac
     4621 cgtacggaag ctgatggcgg ccgggtggtg gataccagcg agcttatccg ggtgtacggt
     4681 gagttaagac aggatgagac tcacgagcgg gacgagttga gactgtcggg tgagactgaa
     4741 aaaatggcct ccgacgcgtt gatcgcggag ataaaagcac tcagggagga ggtggccggg
     4801 ctacggcagg aaatgcagtc catgagactg ctggaacaca aaccggagac agcagagacc
     4861 ccggccggaa caccgcgccg gtggtggcag tggggaaaac gctgacgcgc tgtgcttgtc
     4921 caaccggcga cgaccacgca atgaattgcg tccccgtcac cggttattag agaacaatat
     4981 ggggattctg gcgctggccg accacggcca gaacccggac ttttctggtc tggtcatcaa
     5041 catcatagag gacatgctgt cccatcagac tgatccggcg aacgtcctcg ccatagcgtg
     5101 agtccacttc atagagtcgc gggaagtcgg ccagtttgtc cgccgcagcg agaacccggt
     5161 cggtaacgtt ctgcgcatcc cacagggatg aattttccgc gatatagtca aacagcgtcg
     5221 ttacatcctc gatggctccc tgcttccatt ccacgtcata cggcatcgcg cagttcctga
     5281 taacggccat gcaggcgctg gcggagttgt tccgtggtca tacccggacg cgtgtcagca
     5341 cgggcggcgg tcactttggc gcgaacctga tcttcatact ctgcctgggc cagttcttcg
     5401 ttagccagtc gctgcaacag cgcagctttg tccgggccac cttccggcag tatgcggggc
     5461 aggctttcag ccagttcccg aagaaagttg gcgttttcgt agtgtgtagt gttgctcatt
     5521 gcggagcctc ccgggttaat gcggcaatat gaaggaagtt tagcaggggg cggtctgaaa
     5581 gaatatggtc gtgtcagtca ggctgatgaa gcagaaagtc ggtaaaaaag ggcttacggc
     5641 atccatttta cgtcaaaaca tatgccatgc agttgcgtgc ataggcatgc attaggataa
     5701 aatttatcgg gcgcgtttcc ggcagttttc cgggggggtt gttgcctgtt ttatcccgta
     5761 gccgccggaa acgccctgag cccgtctgag cggtgcgcgt aatgacgcgt tatggtaaat
     5821 ccacttactg tctgccttca tagccatcga gataaacagc tgaaatggtg tcagccagta
     5881 gccttgattt tttcggtgtg aggtctttgc tgcggtaatc ggtacccgca aattgtgttg
     5941 tgccttggag tgagtttaaa aattcctgac tgtgaaactg ctgaatgcgt tgctgcacat
     6001 cactccgtga ttttccggtc gctttatcct ttgcgcccat atcccagaac tgccggaaaa
     6061 aaggcaccat cattcgtgtt gtgtcagctt tctgggtgga aaaattggga tcaaaaccgt
     6121 catctgaagc atatacgtaa tgccgggtat gctgttccac gctgcccttt ggagcacagg
     6181 cagtgagagt tgctgtcgct gttatcatgg cgataagaca cagtttgctg ccggaacgtc
     6241 tcatgagcgt ctccttacct ttaattcttt tttctcattg agttatatat ccgcaggcta
     6301 tcttatcatc agtttctgac cgtgttcagt gaccgaacaa cccgaaccag accccccgtt
     6361 tcttttcctg tcccggaggg ggctttctgg tatctcatat aatgatttca gtaaaacata
     6421 ctgaggccgg agaacggcgc cgtttgtgct ggtttttgtt gcatctggag gggattgcgc
     6481 atggtgtgag tggctgtttt tttgatcatt ttcacaaaaa cgcaaattta cagacttgac
     6541 atgaaaaaca aggcagcgta atttttgtac tgtatataaa accagtggtt atatgtacag
     6601 tattttattt tagtttattg ttttaaaagt caaagaggaa aagtatatga gtggcggaga
     6661 cggacgaggt ccgggtaatt caggtctggg acacaatggt ggtcaggcca gtgggaatgt
     6721 gaacggtaca tcaggtaaag gtggcccttc atcaggtggt ggtacggatc caaacagcgg
     6781 gccgggctgg ggtacgacgc atacacctaa cggggatatt cataactata acccggggga
     6841 gtttggtaac ggcgggagta aacccggtgg taatggcggt aacagcggca atcatagcgg
     6901 gagctctggt ggcggacagt cttcggccac cgcgatggcc ttcggtctgc ctgccctggc
     6961 cactccgggg gctgaaggac tggctttatc cgtttccggc gatgcgttgt cggccgctgt
     7021 tgctgatgtg ctggctgccc tgaaaggtcc gtttaagttt ggtctgtggg ggattgcgat
     7081 ttacggagtg ctgccttctg agatagcaaa agatgatccg aaaatgatgt caaaaatcgt
     7141 gacgtcattg ccggcagaca cggtaacgga gacgccggtc agctccctgc cgctggacca
     7201 ggcgacggtc agcgtcacta aacgtgtggc ggatattgtg aaggacgagc ggcagcatat
     7261 tgcggttgtc accggccggc caatgagtgt tcctgtggtg gatgcgaaac cgacaaaacg
     7321 tccgggggta ttcagtgtgt cgattccggg tctcccgtct ctgcaggtga gcgtacctaa
     7381 aggtgttccg gccgcgaaag ccccgccaaa aggcattatt gctgaaaaag gtgattcacg
     7441 tccggctggt tttacggccg gtggtaactc ccgtgaggcc gttattcgtt tcccgaaaga
     7501 gagcggacag aaaccggtct atgtgtcggt gacggatgtt cttactccgg cacaggtaaa
     7561 acagcgtctg gaggaagaaa agcgtcgcca gcaggcatgg gacgctgctc acccggaaga
     7621 ggggctgaaa agagagtatg ataaagcgaa agctgagctg gatgctgaag ataaaaatat
     7681 tgcgacctta aacagtcgca ttgcatcgac agagaaggcg atccccggtg caagggctgc
     7741 tgttcaggaa gccgataaaa aggtaaaaga ggcagaggca aataaagatg attttgtgac
     7801 ttataacccg cctcatgaat atggctccgg gtggcaggat caggttcgct atcttgataa
     7861 ggatattcag aatcagaatg agaaattaaa agctgcccag acatctttaa acgaaatgaa
     7921 tgaatcctta tccagggata aggctgcgct ttccggggcg atggagagcc ggaaacaaaa
     7981 ggagaaaaaa gcgaaggatg cagaaaataa attaaatgag gaaaagaaaa aacctcgtaa
     8041 gggaactaaa gattacggcc atgattattt tcctgatccc aagactgaag atattaaggg
     8101 gttgggagag ttgaaagagg gtaaacctaa aacccctaaa caaggtggtg gtggtaagcg
     8161 ggcccgatgg tatggtgata aaaagcgtaa aatttatgaa tgggattccc agcacggtga
     8221 gcttgaaggg taccgcgcca gtgatggcga acacctcggg gcattcgatc caaaaacggg
     8281 taagcaggtt aaagggccgg atccaaaacg aaacattaaa aaatatcttt aagaggtaaa
     8341 tatggggctt aaattacata ttcattggtt tgataagaaa accgaagagt ttaaaggtgg
     8401 tgaatactca aaagacttcg gtgatgatgg ttctgtcatt gaaagtctgg ggatgccttt
     8461 aaaggataat attaataatg gttggtttga tgttgaaaaa tcatgggttt cgatattaca
     8521 gccacacttt aaaaatgtaa tcgatattag taaatttgat tactttgtat cctttgttta
     8581 ccgggatggt aactggtaag caaaagttat caaggatgat ttgaaataca gacttccggt
     8641 gttcagggat gaataccggg gtttttaaat acagggaagg tatatgaaaa aggtaaaggt
     8701 tgtttttctt tttatattga ttttttcagg ctttcttctg gtcgcatgtc aggcaaacta
     8761 tatccgggat gttcagggtg gaacggtggc accatcgtcc tcctctgaac tgacggggat
     8821 cgcggttcag tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8881 ttttgccctg taaacgaaaa aaccacctgg ggaggtggtt tgatcgaagg ttaagtcagt
     8941 tggggaactg cttaaccgtg gtaactggct ttcgcagagc acagcaacca aatctgtcct
     9001 tccagtgtag ccggactttg gcgcacactt caagagcaac cgcgtgttta gctaaacaaa
     9061 tcctctgcga actcccagtt accaatggct gctgccagtg gcgttttgcg tgcttttccg
     9121 ggttggactc aagtgaacag ttaccggata aggcgcagca gtcgggctga acggggggtt
     9181 cttgcttaca gcccagcttg gagcgaacga cctacaccga gccgagatac cagagtgtga
     9241 gctatgagaa agcgccacac ttcccgcaag ggagaaaggc ggaacaggta tccg
//
