LOCUS       NZ_CP076864            11849 bp    DNA     linear   CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain V3 plasmid unnamed1.
ACCESSION   NZ_CP076864
VERSION     NZ_CP076864.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19730657
            Assembly: GCF_019038575.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11849)
  AUTHORS   Janssen,L., Almeida,F., Campos,T., Pappas,G., Martins,V. and
            Damasceno,T.
  TITLE     A novel multidrug resistant, non-Tn4401 genetic element
            (NTEKPC)-bearing, strain type of Klebsiella pneumoniae isolated from
            an urban lake with drinking and recreational water reuse
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11849)
  AUTHORS   Janssen,L. and Almeida,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUN-2021) Department of Cell Biology, University of
            Brasilia, Campus Universitario Darcy Ribeiro, Instituto de Ciencias
            Biologicas, Bloco K, 1 andar, Brasilia, DF 70910-900, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7-b1585
            Assembly Name                     :: KpV3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 470.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:23:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,343
            CDSs (total)                      :: 5,223
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 84 of 216
            Pseudo Genes (incomplete)         :: 57 of 216
            Pseudo Genes (internal stop)      :: 102 of 216
            Pseudo Genes (multiple problems)  :: 20 of 216
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076864.1.
            Bacteria and source DNA available from Laboratorio de Analises
            Moleculares de Patogenos, Block I, Institute of Biological
            Sciences, University of Brasilia, Brasilia, Brazil.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11849
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="V3"
                     /isolation_source="lake"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
                     /geo_loc_name="Brazil: Paranoa Lake, Brasilia"
                     /lat_lon="15.740962 S 47.881235 W"
                     /collection_date="2019-02"
                     /collected_by="Luis Janssen"
     gene            485..1649
                     /gene="sopA"
                     /locus_tag="KRP25_RS26155"
                     /old_locus_tag="KRP25_26155"
                     /pseudo=""
     CDS             485..1649
                     /gene="sopA"
                     /locus_tag="KRP25_RS26155"
                     /old_locus_tag="KRP25_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /name="sopA"
     gene            1652..2617
                     /locus_tag="KRP25_RS26160"
                     /old_locus_tag="KRP25_26160"
     CDS             1652..2617
                     /locus_tag="KRP25_RS26160"
                     /old_locus_tag="KRP25_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition protein"
                     /protein_id="WP_217049233.1"
                     /translation="MKQRSILKNAPNIDSIMSKTHRAPASQPVSPMVGDLQRQLSSLSG
                     NSIMLPVCGRNVNFKLETIPADKVEMATMVWLGNERDQDLLNETSLADLVPSFLTSGQQ
                     NPAFARKTAGIIEVADGSRRRKTAIITGCDYRVLVGDLDDEQMQWLSQIGNDYRPTSAY
                     ERGKKYQRRLKDFDGSVKALAEAEGVDRNIITRCINTAGLPKDIIAIFQHPGELSARAG
                     HDLFKIYQGNEKAMLDAAQQLLRMKKQGEKFEPMRIIRALQDFIKTNAKDTQKTEKAYG
                     EGVVAKYNGNYVTLKFDNRKIPSSLMKKIEALLESELEKK"
                     /name="ParB/RepB/Spo0J family plasmid partition protein"
     gene            complement(2696..4195)
                     /locus_tag="KRP25_RS26165"
                     /old_locus_tag="KRP25_26165"
     CDS             complement(2696..4195)
                     /locus_tag="KRP25_RS26165"
                     /old_locus_tag="KRP25_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004148023.1"
                     /GO_component="GO:0098024 - virus tail, fiber [Evidence
                     IEA]"
                     /GO_process="GO:0046718 - viral entry into host cell
                     [Evidence IEA]; GO:0019062 - virion attachment to host cell
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="WP_217049235.1"
                     /translation="MAMYEVGTVTGAASQARVTGATTKWSQEALGILPGSILVVYRSGS
                     ADLYAIKSVDSDTQLTLTRNITTAFSGASYGIITAETASTSSFANQLASAFAFWRSVVE
                     GWSMALTGSGNITLTDPITGKQVTVPAIAGMAKASDLNALAKLTGGNKLDGSQVITSDN
                     AGFILGKNSDLALLKKQGQGGTIAVGSGTPFRVQRSRATTVSPADTFDDILVIGTNNQT
                     TLPGDLVVGGGFDNTAKGKLYSQALELSMGTPYIDFHFNYSTDDFTGRIMATAADQISV
                     QGSHWRVDRDLRVGGMADIGGWAQCGVDLSANRTDFGSPADGSLVSGGRIRSRMLGRGG
                     NGDTSGAWGGFYVEEYVGTEHRIIMYMDGFGRTDAWSFRAGGVISTPKGDVMTTGSDVR
                     LKTDFTQAPGNASERIERLGVCEYRMKGETRRRRGFIAQQAEKADDLYTFLGIEQEIDG
                     EKFKVMNVDYTAIIADPVTVAQGLLVKNQELERRISVLEGI"
                     /name="tail fiber domain-containing protein"
     gene            complement(4263..>7139)
                     /locus_tag="KRP25_RS26170"
                     /old_locus_tag="KRP25_26170"
                     /pseudo=""
     CDS             complement(4263..>7139)
                     /locus_tag="KRP25_RS26170"
                     /old_locus_tag="KRP25_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317731.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /name="DUF1983 domain-containing protein"
     gene            complement(7333..7992)
                     /locus_tag="KRP25_RS26835"
     CDS             complement(7333..7992)
                     /locus_tag="KRP25_RS26835"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228744098.1"
                     /translation="MVRAYFTLTNSWARYWVKWKQTPTTGTKRLILCRIESNTSKDQTV
                     YINSPKFEVGNVVSDWNESPSDSASASAVDLLTTAVNQQGTSISSIGNRTTSLENGLST
                     TNANVAKKADASSLQTLQNTVTQQGNDISSRGTRVTVIENSLTSGANLIPNAKMLNNAQ
                     GWDGNATTVDGYAAVSSARGWQPSSAPFEVTPGDTLDLSLMCLVDAAITFASGVAL"
                     /name="hypothetical protein"
     gene            complement(7940..9163)
                     /locus_tag="KRP25_RS26840"
     CDS             complement(7940..9163)
                     /locus_tag="KRP25_RS26840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228744099.1"
                     /translation="MNSLTGRVEKTESGLTAANSNITSLSSSLSQQSKRGANLLPDGTF
                     ESYAVGHNLANNRAIVTTDDSHGGNKCIRVTRPNDYNANATDNTDTHIFSGFRVRDNAV
                     FYMECWVKLDAKSTAMAENVQISVGLSLQYQDNSRQWPAVTKAAKDLSSTQWTKVSGYL
                     KSTKSGIKQAMVRISIPNVSSVKAGNSFLIDDSVITEVTDAYNAQSTADANANAISTLN
                     STVSQQGDQITSQGNSITELTNDPATTNNNVSNKADANALTALTNRVTQTEKDINSTSS
                     SVTNLNNKVDSISVGGTNLIKNSGDMTGWSNVTSETYRGNAVIGATVKAGSGYKDLREI
                     TLESPVDAGEYVYSFYAKGGVAGQTMTAFFYNPNTTTSIETSRGAKGNNTDGTCVLHAD
                     QFMGSLLG"
                     /name="hypothetical protein"
     gene            complement(9051..9401)
                     /locus_tag="KRP25_RS26845"
     CDS             complement(9051..9401)
                     /locus_tag="KRP25_RS26845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228744100.1"
                     /translation="MNQWTGQAYINLGLKLSDKVAEMYSGLGVSALANAAGVLSSNVSQ
                     IGNDVVSNSQSITQLRNALTQTDANVAQQSGSDGDELANRTSGEDGVRSDGGEQQHYVA
                     QQLSEPAIQTRR"
                     /name="hypothetical protein"
     gene            complement(<9751..>11849)
                     /locus_tag="KRP25_RS26185"
                     /old_locus_tag="KRP25_26185"
                     /pseudo=""
     CDS             complement(<9751..>11849)
                     /locus_tag="KRP25_RS26185"
                     /old_locus_tag="KRP25_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317731.1"
                     /GO_function="GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /name="carbohydrate binding domain-containing protein"
ORIGIN
        1 ctccgttatg ttatgatgct tgactatgtt ctctggattg gttacgatat caatcattgt
       61 gattaaccta tgatatgcaa ttgtgttatg atgttggttt tggtgatcaa gttaaggaat
      121 ggggaagcct aaagttttct ttgtgtttat gttgtctgat agtgttacgt tgttgtggtc
      181 tatttatcgg ttgatgtcct ttagataaaa agggcctcga aagcagcctg ttatcttccg
      241 atagagttga agacagataa ccattttatc tataaaaaac ataaagttag gttttacact
      301 tcttcttcta acttaccttc tactcataat gcatcggcta agcacgctaa aagcctgttt
      361 ctgttggtta aatcataaac tttgcttttt atcatcaact ttgcgttttt tacgttgctt
      421 tgcgttaaat acataaagta cgataaaaaa cataaaggaa aattactcct caagggggat
      481 tggaatgtcg cttattaatt tactgcatga atgtatcagc cgcgggcagg agatgacgca
      541 ggccattgct atagctcaat ttggtgatga tagccccgag gctcgccgta tcactcgtcg
      601 ctgggggata accgaggttg ctgatttaat cggcgtttcg ccgcaagcaa ttagggatgc
      661 tgaaaaaaaa tgggcgacta ccaccacctg attttgagtt acgcggtcgc gttgaacgtc
      721 gcgccggcta taccatcgac cagattagcc atatgcgcag cattttcggc aatccgaatc
      781 aacggcctgc agacaaaaat ccagctgtgt tagctgttat gtcgcacaaa ggcggcgttt
      841 ataaaacctc atctgcagtg catgaagctc aatggttagc tctgcaaggt caccgagtgc
      901 tactcattga aggtaacgat ccgcaaggga ccgcctcgat gtatcacggc tatgtaccgg
      961 atttacacat ccatgccgag gatactttgc tcccgtttta ccttggtgag cgtgataatg
     1021 cggaatacgc tataaaaccg acctgctggc caggtctgga cattatcccc agctgcctgg
     1081 cgctgcaccg tatcgaaaca gatctgatgc aataccatgc ccaaggaaaa ctacctcatc
     1141 ctccgcatct gatgctacgg gccgctatcg aatccgtatg ggataactat gacatcatcg
     1201 tcattgatag cgctccaaac ttagggacag gtacgattaa tgttgtttgc gcagccgata
     1261 ttatcgtcgt ggctacccct gcagagctat ttgattattc atccgttctg cagttcttca
     1321 ccatgcttct ggaccttctt aaaaccgttg atttaggtgg ttttgagcct gttgtacggc
     1381 tgttactaac gaaatacagt ctgaccactg gtaatcaatc gcgctggatg gaagagcaaa
     1441 tcagaaatac atggggatcg atggtcctgc gtcaggttgt ccgagtgacg gatgaagtag
     1501 gaaagggcca aataaaaatg cgcacagtat ttgaacaggc cgcgaaccag cgttcaacgc
     1561 ttaatgcatg gcgaaatgca gtctctattt gggagcctgt ttgccaagaa attttcgatg
     1621 acttgattaa acctcgttgg gaggactaat tgtgaaacag cgctctattt taaaaaatgc
     1681 ccccaacatc gacagcatta tgagcaagac gcaccgggcg cccgcatcac agcctgtgtc
     1741 accgatggtg ggtgatttgc aacgccagct tagttcatta tcgggaaaca gtattatgct
     1801 gcctgtgtgt ggacgtaacg ttaactttaa gctggaaact atcccagcgg ataaagttga
     1861 aatggcgacg atggtttggc tcggtaatga acgtgatcaa gacctgctca acgaaacgtc
     1921 cttggccgat ctcgttccgt cattcctcac ttcggggcag cagaacccgg cgttcgctcg
     1981 caaaactgca ggcatcatag aggttgctga tggttctcgg cgccgaaaaa cagccatcat
     2041 tacaggttgc gattatcgcg tattagtcgg tgatctcgat gacgaacaaa tgcagtggct
     2101 ctctcaaatc ggcaacgatt atcgaccaac aagcgcatat gagcggggga aaaaatatca
     2161 gcgccgcctg aaagattttg acggcagcgt aaaagcttta gctgaagcag aaggggtgga
     2221 ccgcaatatc ataacccgct gcatcaacac agctggcctg cctaaagata tcatcgccat
     2281 ttttcaacat cctggtgagc tatcagcccg tgcggggcat gacttgttca agatatatca
     2341 gggaaacgag aaagctatgc tcgatgcggc gcagcagctg ctacgcatga agaagcaggg
     2401 tgaaaagttc gaacctatgc ggatcatccg agcattacag gattttatta agaccaatgc
     2461 aaaagataca caaaagaccg aaaaagcata tggtgaaggg gttgttgcga aatataacgg
     2521 taattacgtt accttaaaat ttgataaccg gaagattcca tccagcttaa tgaaaaaaat
     2581 tgaggcgttg cttgagtcag agttagaaaa aaaataaacc acataagaaa aaaggcccct
     2641 gcattgcagg ggccttttat gttctgtttt ttattagcgg ctaatcgtag ggcaatcaga
     2701 tcccctccag tacagatata cgcctttcca gttcctgatt tttaaccagt aaaccctgcg
     2761 ccaccgtaac cgggtcagca atgattgccg tgtaatccac attcatcacc ttaaattttt
     2821 cgccatcaat ctcctgctcg atgccgagga aagtatacag atcatcagct ttttcagcct
     2881 gctgagcgat aaaaccacgc ctccggcgcg tttccccctt catccggtac tcacacaccc
     2941 ctaagcgttc aatgcgctct gaggcgtttc caggcgcttg tgtgaagtcg gttttcagtc
     3001 gcacgtcgga accggtcgtc atgacgtcgc ccttgggcgt ggaaattacc ccacctgcgc
     3061 ggaatgacca tgcgtcggtt ctcccgaagc cgtccatata catgataatc ctgtgttcgg
     3121 ttcccacgta ctcttcaaca tagaaaccgc cccacgcccc ggaggtgtca ccgttaccgc
     3181 cgcgtcccag cattctggat cgaatacgtc cgcctgaaac caacgaacca tcagcagggg
     3241 aaccaaagtc tgttctgttg gccgaaaggt cgactccgca ttgcgcccac cctccaatat
     3301 ctgccatacc accaacacga agatccctgt caactcgcca atgactacct tgtacactaa
     3361 tttgatcggc ggcagtggcc ataatccgcc cggtgaagtc gtcggtgctg tagttaaaat
     3421 gaaagtcgat gtacggagtg cccatcgaca gctctaacgc ctgggaatac agcttgccct
     3481 ttgccgtatt atcgaaacca ccaccgacaa ccaaatctcc gggcaacgtc gtctggttgt
     3541 tggtgccaat aacgaggatg tcatcaaagg tgtctgccgg tgatacagtg gtcgctcttg
     3601 aacgctgaac cctaaacggt gttcccgagc caacggcaat tgtcccgcct tgcccctgtt
     3661 ttttgagcag agccagatct gagttcttac cgagtataaa accggcatta tcgctggtta
     3721 taacctgcga gccgtcgagt ttgtttcctc cggtgagttt tgccagcgcg ttaagatccg
     3781 aggccttcgc catcccggct atcgccggca cggtcacctg ctttcctgtg atcgggtcag
     3841 tcagggtgat attgccgctg ccggtcaggg ccatcgacca gccctccact acactacgcc
     3901 agaatgcaaa cgcgctggcc agctggttag caaacgacga ggtgctggcg gtttcagcgg
     3961 taataatgcc gtaactggca ccggaaaatg cggtggtgat attccgggtc agcgtcagtt
     4021 gcgtgtcgct gtccacggat ttgatcgcat acaggtcagc actaccgctg cggtagacca
     4081 ctagaatcga cccgggcagt atccccagcg cctcctgtga ccattttgtt gtcgcacctg
     4141 tcacccgtgc ctgcgacgcg gcacccgtga cggtaccgac ttcatacatc gccataataa
     4201 agttcctccc ggatggtttt cctcggaaaa gaaaaggccc cttgcggggc catatattgt
     4261 tatcaggttg atgaaccgcg aattacgttg ttattgcgcg cggcgaaggc gtcgaatctt
     4321 gagaggatgg ttaataccat ctgtccgtta cggtccggta acagccatat cgcagaacag
     4381 cgtcgccagt accggcaggg acgacaaatg acatgttgtc tacgttcatt gacccttgcg
     4441 ccgttaacag gcgtgttgct ccacctgttt catacataag ctcaatgcct agctccatac
     4501 tgccgccacc agacgtagcg cctgtgccac gaagaagcat tgcatacgac agatagccgg
     4561 gattcagtcc gccgtcagcc cctggcgcgt aggacatccc cgctctgagt ttcgcgccgc
     4621 cgtcgatagt gatgctatcc gtctggagga gtccaccgcg aatattgatc acctggtcga
     4681 aattctgccg ccggaaccgg gccagctcaa accactgcca tgtagccttc ggcaccttgc
     4741 gcgtgcggtg ctgagcgatg ttgatcgcaa atgacccgat gtcgccctcg atgtggttcg
     4801 catataccgt accccggaac cagccgtcag tcgcataaac cgcaccgcga acgatcacgt
     4861 tgttgaactg cgaagagcca tccttagcga tacgccagcc gcgtgacccg tcaacaaagt
     4921 cattcgagcg gatctcgtgg ccgatcttcg cgttcgtgat ggaaccgtcc gcgattttgg
     4981 ttgaggtcag ggaactgttt ttgatacgtg ccgtatcgat atacagctca ttgccttcgg
     5041 caaccatcac cggaacagcc gtcgcattat tacggttaaa cagcgagaag cggtcagcgt
     5101 agaggatcat atcgcttgtt tcaccattgc tgcccagcgt aatccccgcg ccaacattct
     5161 tcccgttaac cgtctcaacc ttcatcgacc acagcgaact caccgtacca ttcacatccg
     5221 ccacggtttt ggcggtgttc tgaacggaag cgctgagatc cccgacactg gatgtcaggg
     5281 tcgtctgctg cgttgccagc gcctccagtg ccgttgcatg cgtctgctgg gtactggtga
     5341 tactggccac cgatttaatc gtgttgtcca gcgtcgtctg gtttttgatg ttggcggccg
     5401 cctgcgcgtc aatctgcgac tgaagcgagg tattcaggct ggcctgtgtg ctctggctgt
     5461 cgctcagcgt cttcgccatg ttatcgacgc gggagttggc gttatccact ttcgaggcca
     5521 gtgctgtctg ctgctgcgcc tgggcagtga ttttcccttc ggcatccgtt acgcgcgccg
     5581 tcaggccgct cacggcgctc gccgtcgcgt cagaggcatc ctgtgccgct ttcgcatcgg
     5641 taacatccgt gatgaccaga tcgtcgatat acagtgaata gccggggtac cgttgccggt
     5701 ggcgccacga gtggagatcc agaccaccgc gcgtgttctg cctgccccgt tgttactggc
     5761 gatacccgtg aatttcaccc acttatcgcg cgcgcccaga gaggcttcgc tgacagtgac
     5821 cgccgcctgc cacgcatttt gcccggcagc attctgcgac tggataccga ccagcgttgt
     5881 ccacccggag gagggcgcct gatccgccgg catcatagcc cagaactcaa accggaactt
     5941 cgcatcctca cggactgact gccagctccc aagctgttta tcgctgttgc cgttgtcgtt
     6001 cgctcctcga ctcacctgca ggctcttatt gccggtgaat ttctgagacg ccaccacaac
     6061 ggcggtgccg cccccgccca gcacatgacc atctccgtag ctttcgaacg taccgtcaac
     6121 ccacggatta gctccctgag tactgatggt attgatgctg ctggtcagcg acgtgatact
     6181 ctgcgactgg ctggtgatag tgttttccac cggcttacgc gaccagtcag tgaactcact
     6241 gcagtcgtgt cagccttttt actcaccgta tcgtttgtcg acctgaggct gttctccagc
     6301 gccgttatcg aggaactcag ggagtcaata ttacccccct gggtcttcac tgtgttctgg
     6361 agcgtggtaa ttgccgacgc gttcgcatca gccttcatca tcacaccgcc ggctgcaccc
     6421 agccccatca ttatcccgtt cacaaattcg actgaggtcg aaatgtgagc ggtggcatct
     6481 ccaccggttg gcgcacgcag ttccagacca tcgcccgacc tcatgccttt gcggccaagg
     6541 agaatatagg caccacgata cggcagggag ttgacgactt cggatgtacc accaagagat
     6601 tccagagcag acaatatctt acctcggttg ccagatggct catcgaatgt caggacgcaa
     6661 acgtaagtgc cactagtcaa cgccccgata tcagccgaca tcgtgtcgcc attattcgcg
     6721 ctgccaaaga catcgtatgt tttggaggtc gcaatcaccg ctgatccgtc gctttgtttt
     6781 gcaaaagtga ccagcgccca gctgcgacca ggggtaaaca ggtttttgcc gctttcatca
     6841 aaaaccccgg gagttacgct gttgccattc ccccgtgcag tgacagtaaa cacagtgcga
     6901 cggttcatcg aggcctgcag gctggtaatg ctactgttcg ccgcagttaa atcgcccccc
     6961 tgagatgtca ccgtgttccg taaatcctgc aatgcagaag catcagcctt cttaccgata
     7021 tcgttctgag cggtagccag tccgttttcc agtgaggtgg ttctattacc aatagagctg
     7081 attgcatcac cctgcgtctt aaccgtactg gtcagcgagt caatagcgga actattcgct
     7141 atagtcgttg cgtctcgacg ggtaacaacg acgttataga ttgtgacacc cgcggtaacg
     7201 cctatgttcg gttgcagcct tgctgtggtc gcgccgacag gaacgacaaa cgtaccgctt
     7261 acagccttct tctcaccagc ttgataactc aagttgtttg tgtagacggt tttattgttg
     7321 agactaggtc cgtcaaagcg caacccccga ggcgaaagta attgccgcat ccaccaaaca
     7381 catcaggctt aaatccagcg tatcgcccgg tgtcacttca aacggtgcgg agctcggttg
     7441 ccatcccctt gcgctgctca cagcagcata accgtcaaca gttgtcgcgt tcccatccca
     7501 cccctgtgcg ttattgagca tcttggcgtt agggatgagg ttagcaccag acgtcaggct
     7561 attctcgatg accgttacac gagtgccccg gctggaaatg tcattaccct gctgcgtcac
     7621 cgtgttctgg agtgtctgca gggatgaagc atctgccttc ttcgcgacgt tagcattagt
     7681 ggtgctaaga ccattctcca gcgacgttgt gcgattacca atagagctta tggaagtgcc
     7741 ctgctgattc actgccgttg tcaacaagtc cacagccgac gcgctggcgc tatcagacgg
     7801 agactcgttc cagtcggaaa caacgttacc tacctcaaac ttcggactgt tgatgtacac
     7861 cgtctggtct ttggaggtat tactctcgat acggcacaga atcaggcgct tggtacccgt
     7921 ggtaggtgtc tgtttccact taacccaata gcgagcccat gaattggtca gcgtgaagta
     7981 cgcacgtacc atcggtgtta ttacctttcg cacctcggct ggtctcgata gacgttgtgg
     8041 tgttcggatt gtagaagaac gccgtcatcg tctggccggc aacaccgcct ttcgcataga
     8101 agctgtaaac gtactcacct gcatcgacag gcgactcaag cgtgatttcg cgcagatcct
     8161 tgtaaccgga gccggctttt accgttgcac caattaccgc gttaccacga tacgtctcgc
     8221 tggtaacgtt cgaccagccg gtcatatcgc cggagttctt gatcaggttt gttccaccga
     8281 cagaaataga atcaaccttg ttgttcagat tcgtgacaga cgaactcgtt gagttaatgt
     8341 ctttttcggt ctgggtaaca cggttggtca gtgccgtcag agcattagcg tctgctttat
     8401 tgctgacgtt gttattagtc gtcgccgggt catttgtcag ttcagtgatg ctgttaccct
     8461 gactggtgat ctggtcgccc tgttgggata cggtcgagtt cagtgtcgaa atcgcattag
     8521 cgttagcatc tgctgtactt tgcgcattgt aggcatcagt cacttcggta atgaccgagt
     8581 catcaatgag gaatgagtta cccgccttaa cgctgctaac gttaggaata gaaatcctga
     8641 ccattgcctg cttaataccg ctcttcgttg atttcaggta accagaaacc ttcgtccatt
     8701 gagttgaaga gagatccttt gccgctttgg taactgccgg ccactgccgg gagttgtcct
     8761 gatactggag cgataagccg acggaaatct gcacgttctc ggccatagcg gtactcttgg
     8821 catccagctt aacccagcat tccatataga agactgcgtt atcgcgaacc cggaatccgc
     8881 tgaaaatgtg ggtatcagtg ttgtcagttg cattggcatt gtagtcattc ggacgcgtca
     8941 cacgaatgca tttattgccg ccatgcgagt cgtcagtggt cacgatggca cggttattcg
     9001 ccagattgtg gccaaccgcg tagctttcaa aagtgccatc aggaagcaga ttagcgccgc
     9061 gtttggattg ctggctcaga gagctgctga gcgacgtaat gttgctgttc gccgccgtca
     9121 gaccggactc cgtcttctcc actcgtccgg ttagcgagtt catcgccgtc tgatccgctt
     9181 tgctgtgcca cattcgcgtc tgtctgcgtc agcgcattcc ggagctgggt gatgctctgc
     9241 gaattgctga ccacatcgtt gccaatctgg ctgacattcg agctgagtac gccggctgcg
     9301 tttgccagcg cggaaacacc gagaccggag tacatctcag caaccttgtc tgacagcttc
     9361 aggcccaggt tgatatacgc ctggccggtc cactgattca ccagaaactc aacggtattc
     9421 cagccgactt tcagttcaaa actgccggta gtccagctgg cattccccga gcaacctgaa
     9481 cgccatttac aaatacagcg ccggtatcat caaaaatcct gttaccgggc gccattgtga
     9541 tggtggtatc ggcggccact ttcaccggca ggaatacagc gcgatcagat agctgccggc
     9601 ggacgtaaag tccagtttgg ccgcgtcggc cacctcatcc acgaccacgg gcgccacggc
     9661 gcgaatatcg ctgaatgacg ggactgtccc ggcgttagcc agctgcacag gatagatccg
     9721 acgggaccag ctattcggct ggccattgac cagctgattc gacagactgg tgatgctgtc
     9781 agtattgctg cgaatattct ggccgttttg ctctacctgc tgtgttaacg cggtgaccgc
     9841 gctcgcctcc gctttcttcg ccagcgcggc atttgtcgtg ttcaggctgt tctgcaggtt
     9901 cgtcagctgc tggctttgcg aggtgatatg cccttccgct gtactgaccc ggttcgttag
     9961 tccggtaacg gcgccagcgg tggcatcgat gtccacccgg tcggtaacgt cagtgacgta
    10021 aaaatcatcg aagtagcggc ttccgctaat cagatagttg ctcatcgtca ccggcaggct
    10081 ggctgtctcc gtcgctttcc agcgaccgga aatcagggtc cagtttgtcc ccaccgtacc
    10141 gctgttatat ggacgctcaa aaaccggctg gccggcagag ttaccgatcc gcagcttgtt
    10201 gttccccgcg ccattatccg tcgtcgctcc gggttccttg acccacaccc cgatttcata
    10261 ggttcgcccc tgaacaaacg ggatgtattg ccccggagac acgcttcccg gatcaacctt
    10321 cagcgcccgc gtcccgctgt gaggagcgga aacctccacc acactggtcg cggttgaccg
    10381 cccggtataa ccatccagcc cgcgttcaaa cgagggattc acgaccaggt tacccggtat
    10441 ctgcccgctg gcatcgatat ctgccgcgac ctgcgagagg ctgttcgaca ggttcgtcag
    10501 cgaattgctc tggctctcca gcgttttgcc ctgctgcgtc actttcgtgt cgagcgtggc
    10561 cagcgcagtc gcatcggctt tctgcgccag cgctttatcg gtattcgcca gatttccggt
    10621 cagcttcgtg atggcgctgt tcgcagccgt caggtcattg cccagctgtt tgacggtatt
    10681 ggtcaaatcc tgcaccgctg tcgcatcagc ctttttggcc actgcggcat tggtggttgc
    10741 cagcccgttt tccagctggg ttgtccggtt gccggtcgag gtcagcagat taccctgttg
    10801 agtcacggtg gtggtcaggg agtcaaccgc cgccgccgtg gcgtccgcag tatcctgaac
    10861 cttttgcgcc gctgtcacat ttcgcatatg ccagtccgta acgaaccata cggtgccata
    10921 cgggctgttc tgcgagatct gcaggaacgg gcggatataa cccctgtcca ccatcgcctg
    10981 cgtgaccttg aagcgccggt ggttctctgc caggtcgcgg agggggattt tccgccaccc
    11041 gccatgagtg gcgcaccggt gctagtatct ggccgaacgg cggtaccaac atacagatta
    11101 aaattcgcgg tgccggcgcc gcaggcaacc agtgcgctga tctcaatcac atcgttaagc
    11161 gtggccggga acgcggcaaa gttaggatgg tgatcccggc tggcaattct ggccgcataa
    11221 ccatacgggc atcccgacgg aacatcctca gccgtcgtgg ataccacact gaaccccatc
    11281 tggtcgtaag ttggatcaaa tgtcgggttg ggaattaaat ccccgcctga tgcgtttccg
    11341 gcccgtacag cggatttcag cgaggtaatg ttggcgttag cagccgtcaa cccggattcc
    11401 gtcttctcca ctcgtccggt tagcgagttc atcgccgtct gatccgcttt gctgaccacg
    11461 ttcgcgtctg tctgcgtcag cgcattccgg agctgggtga tgctctgcga attgctgacc
    11521 acatcgttgc caatctggct gacattcgag ctgagtacgc cggctgcgtt tgccagcgcg
    11581 gaaacaccga gaccggagta catctcagca accttgtctg acagcttcag gcccaggttg
    11641 atatacgcct gtccggtcca ctggttcacc agaaactcaa cggtattcca gcctgctttc
    11701 agttcaaaac taacggtact cgtgcttgcg ttaccccaag cgacctgaac cccattcaca
    11761 aatacagcac cggtatcatc aaaaaccctg ttaccgggcg ccattgtgat ggtggtatcg
    11821 gcggccactt ttacccggca ggaatacag
//
