LOCUS       NZ_CP076840            15048 bp    DNA     circular CON 19-JUL-2023
DEFINITION  Staphylococcus aureus strain 55-100-016 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP076840
VERSION     NZ_CP076840.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19718690
            Assembly: GCF_018987325.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15048)
  AUTHORS   Larsen,J.
  TITLE     Pleuromutilin-resistance in MRSA CC398
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15048)
  AUTHORS   Larsen,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Depaertment of Bacteria, Parasites and
            Fungi, Statens Serum Institut, Artillerivej 5, Copenhagen S 2300,
            Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/19/2023 03:18:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,792
            CDSs (total)                      :: 2,710
            Genes (coding)                    :: 2,618
            CDSs (with protein)               :: 2,618
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 45 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 27 of 92
            Pseudo Genes (multiple problems)  :: 19 of 92
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076840.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..15048
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="55-100-016"
                     /host="Sus scrofa"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /geo_loc_name="Denmark"
                     /collection_date="2014"
     gene            join(14746..15048,1..420)
                     /locus_tag="KRH48_RS13745"
                     /old_locus_tag="KRH48_13745"
     CDS             join(14746..15048,1..420)
                     /locus_tag="KRH48_RS13745"
                     /old_locus_tag="KRH48_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_074347720.1"
                     /translation="MKSVKILSEELGVSKQTIFNNIKRLNIETLKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTAEDKQKKKSDNNVESKSNAQIVEILKKQIDTLNDQIERQES
                     RHETTIEFYRKELQERSKLLENQQILALESNKKIQKLENQLEEERQLNYSFDKVTNDRK
                     NINAQEADFTVEPKNTNQQQEENQTTDYSVISGENKDEDIKKVTTEEEKRVKEQESETN
                     NEVEQPSKKGFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            648..3434
                     /locus_tag="KRH48_RS13750"
                     /old_locus_tag="KRH48_13750"
     CDS             648..3434
                     /locus_tag="KRH48_RS13750"
                     /old_locus_tag="KRH48_13750"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002512569.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:0004386 -
                     helicase activity [Evidence IEA]; GO:0009035 - type I
                     site-specific deoxyribonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="WP_074368327.1"
                     /translation="MGYQSEYGLEENVIKQLQDLGYERVLLRNETQLKENFRRILNERN
                     ADKLEGTPISDSEFKRIMIDISDKSVFESAQILRDKYVLERDDETKVYLSLMNIKKWCQ
                     NTFQVTNQVSVNDTHKSRYDVTVLINGLPLVQIELKRSGVAITEAFNQIERYRRQNYTG
                     LFRFIQMFVVSNKMETRYYANSDQKIMKSHMFYWSDEKNERINVLKDFIESFLEPCHIA
                     KMISRYMIINETDKILMALRPYQVYAVEALLNRAIETNNNGYIWHTTGSGKTLTSFKAS
                     QILAQEPDIKKVIFLVDRKDLDGQTLGEFNKFESDSVDRTFNTKKLLKQMDDPTRKLIV
                     TTIQKMDNAIKSGHPAMERYKEDKVVFIIDECHRTQFGSMHRIIRQHFGNAQYFGFTGT
                     PRFEENASQDGRATADIFGECLHHYLIKDAIRDGNVLGFSVEYMNTFDRSEKIIEDGYV
                     NKINEAEIWMADERVQKVAEHIIANHNKKTRDQMYTGMLTVQSIPMAIQYYRVFQKLKK
                     QGVHNLNVTTIFSYQDNEAIDDTMDEQTRRDILEGIIQTYNETFDKNFSTDTFDGYFND
                     VSNRVKKGLPNQKLDILIVVDMFLTGFDSKKLNTLYVDRNLQHHTLIQAYSRTNRVEEP
                     TKPYGNIVAYRNLKEKTDEAIELFSKTDNTDTVLSLSFQAYLTKFKQALIELFELAETP
                     QAVDQLEREEDQRDFVIAYRELAKHMQKLKTFDEFVFDKETLGIDEQTYEDYKGKYLDI
                     YDKVMRPDKETLEGESILDNIDFNIEILRNDLINVQYILDLLNQIDLSNTEQQEKDIKE
                     IRKVLDKADDDQLRLKSDLIRSFLDKVVPNLNEGDSIDDAYYNYESKVKVGELEQFSEE
                     TDYPLELLKSLVKEFEFSGHLNNNLIEEGVSGGLLIRTKKIKRVKSFIKEMVKKYSV"
                     /name="type I restriction endonuclease subunit R"
     gene            3532..4107
                     /locus_tag="KRH48_RS13755"
                     /old_locus_tag="KRH48_13755"
     CDS             3532..4107
                     /locus_tag="KRH48_RS13755"
                     /old_locus_tag="KRH48_13755"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508614.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="WP_074347714.1"
                     /translation="MLKDYVNYQSGTLIQRLEVDESDLGNRYMIYDQNMLDIDLGLTID
                     DTYQPRVIKLKDTSRATIVHKDDIVISMMTGECTLVSTKHDGSILPYNYTKIEVTSDLL
                     EPAFLVYWFQLAPEVQSQYKQYMQGGSTIKKLTHQQLKSLYITLPSIERQRLIGQIGIK
                     EKQLNVLKQRQSRLKKQFLSNTLFKEDN"
                     /name="restriction endonuclease subunit S"
     gene            4108..5664
                     /locus_tag="KRH48_RS13760"
                     /old_locus_tag="KRH48_13760"
     CDS             4108..5664
                     /locus_tag="KRH48_RS13760"
                     /old_locus_tag="KRH48_13760"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469809.1"
                     /GO_function="GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA]; GO:0009007 -
                     site-specific DNA-methyltransferase (adenine-specific)
                     activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA];
                     GO:0009307 - DNA restriction-modification system [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M"
                     /protein_id="WP_074347715.1"
                     /translation="MSITEKQRQQQAELQKNLWSIADDLRGNMDANEFKNYILGMIFYR
                     FLSEKIEEQAQILLAEDNIDYETAMADEDYRPVLEQEFISRIGYVIEPQYLFGHLVKKI
                     EKQAFEMEDLSNAIKNIENSTRGHDSEDDFIHLFDDLDLNSSRLGNSNAARTKLISKVM
                     MKISTLPFVHSDMEIDMLGDAYEYLIGQFAASSGKKAGEFYTPQQVSTILAKIVTANKK
                     DIKSVYDPTCGSGSLLLRVGREANVRQYYGQEYNSTTYNLARMNMLLHDINYANFKIEN
                     GDTIEDPAISDERFEAVVANPPYSAKWSSDPQFLEDPRFSNYGKLAPKSKADFAFIQHM
                     IYHLDDNGTMAVVLPHGVLFRGAAEGVIREYLIKEKNYLDAVIGLPSNLFFGTSIPTSI
                     LVFKKCREDDDNVLFIDASQSFEKGKNQNHLTSEDVEKIVETYKNRETLDKYSYAASLE
                     EIAENDYNLNIPRYVDRFEEEEPIDLEQVQKDLNQIDDEIVKVEQEIEGYLKELGVLKH
                     D"
                     /name="type I restriction-modification system subunit M"
     gene            5657..6865
                     /locus_tag="KRH48_RS13765"
                     /old_locus_tag="KRH48_13765"
     CDS             5657..6865
                     /locus_tag="KRH48_RS13765"
                     /old_locus_tag="KRH48_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="WP_074347716.1"
                     /translation="MTNQTKNVPELRFPEFDGEWEERKIGNYIVEIKKYDTQQHSSYSV
                     ATSSRRGLFKQAEYFDGNRVFSDKEVLYSVVPPNTITYRHMSDDNIFKFNINSFNESVL
                     VSREYPVFDVNDYADKNFVFYELNNSSNFLKFCIKQKKGGTRTRLYFKVLENYETFYPE
                     KTEQQKIGEFFSKLDRQIELEEQKLEKLEQQKKGYMQKIFSQELRFKDKNGNEYPEWEV
                     IKLKDILSERKEYASKIGNYPHATLSTSGISLKSDRYNRDFLVKDKNKKYKVTIMNDIC
                     YNPANLKFGVITRNHIGSAIFSPIYITFEVNNAHSPLFIELLVTRNDFINRVRKYEQGT
                     VYERMAVKPEDFLNYETKIPCLEEQEKIGDFFSKLDKVINKQRQKIDELKLRKQGLLQK
                     MFV"
                     /name="restriction endonuclease subunit S"
     gene            <7149..7232
                     /locus_tag="KRH48_RS14035"
                     /old_locus_tag="KRH48_13770"
                     /pseudo=""
     CDS             <7149..7232
                     /locus_tag="KRH48_RS14035"
                     /old_locus_tag="KRH48_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /name="peptide-binding protein"
     gene            7281..7364
                     /locus_tag="KRH48_RS13775"
                     /old_locus_tag="KRH48_13775"
     CDS             7281..7364
                     /locus_tag="KRH48_RS13775"
                     /old_locus_tag="KRH48_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /GO_process="GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="WP_001814874.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
                     /name="23S rRNA methyltransferase attenuation leader
                     peptide"
     gene            7489..8226
                     /gene="erm(B)"
                     /locus_tag="KRH48_RS13780"
                     /old_locus_tag="KRH48_13780"
     ARG             7490..8223
                     /name="erm(B)"
                     /gene="erm(B)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8632..9183
                     /locus_tag="KRH48_RS13785"
                     /old_locus_tag="KRH48_13785"
     CDS             8632..9183
                     /locus_tag="KRH48_RS13785"
                     /old_locus_tag="KRH48_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508682.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_031795062.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDVLVIYKLDRLGRTTKQLIELSQWLDENNIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLAAARARGRKGGRPSLPDHKKREIKFLYDEQKWTGE
                     DIAKKTGVSRSTVYRVIKES"
                     /name="recombinase family protein"
     gene            complement(9463..9555)
                     /locus_tag="KRH48_RS13790"
                     /old_locus_tag="KRH48_13790"
     CDS             complement(9463..9555)
                     /locus_tag="KRH48_RS13790"
                     /old_locus_tag="KRH48_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_023016171.1"
                     /translation="MAILFVSIIAPVISGCIVAIFTFWLNNRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(10042..10488)
                     /locus_tag="KRH48_RS13795"
                     /old_locus_tag="KRH48_13795"
     CDS             complement(10042..10488)
                     /locus_tag="KRH48_RS13795"
                     /old_locus_tag="KRH48_13795"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074347718.1"
                     /translation="MEFLELFTRDLLFWQILIVVFGSIVIKKISFPKLHHVEMNQKSTK
                     ALSILSNFFIFIMVSIMICMLILLTLSEVITQISIKEMKEHQQTFYGYLIFLFMYSMYF
                     ALLLSPIWIKKRKFFLLELINKDGEKKYFKLSKRLNYNGIDNLI"
                     /name="hypothetical protein"
     gene            complement(11624..>11797)
                     /locus_tag="KRH48_RS13800"
                     /old_locus_tag="KRH48_13800"
                     /pseudo=""
     CDS             complement(11624..>11797)
                     /locus_tag="KRH48_RS13800"
                     /old_locus_tag="KRH48_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002492756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /name="rhodanese-like domain-containing protein"
     gene            complement(11899..12441)
                     /locus_tag="KRH48_RS13805"
                     /old_locus_tag="KRH48_13805"
     CDS             complement(11899..12441)
                     /locus_tag="KRH48_RS13805"
                     /old_locus_tag="KRH48_13805"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_231411151.1"
                     /translation="MKNIHQDVSKSYANDTDISNTDISNTDISEMNGMSGNNIDSMPEI
                     NDHSLHSNHENDNYLIDEQSHLLKNLPKDLQEFIKSRYILADSISIINKIMQAKREAAN
                     RRGDSLFFMFEENPFLSEKIMDILITVNREMKKKRESVQAMLGYYYNSLVNGLEEFHFT
                     EQARKTVQNKENPFLDI"
                     /name="hypothetical protein"
     gene            complement(13375..14331)
                     /locus_tag="KRH48_RS13810"
                     /old_locus_tag="KRH48_13810"
     CDS             complement(13375..14331)
                     /locus_tag="KRH48_RS13810"
                     /old_locus_tag="KRH48_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_074347719.1"
                     /translation="MSNFKRITIQEFEQERFYKLYKFLFEDEYFRKLSDSSKIAYCIFR
                     DRFELSKMNNWIDENGNVYLIFTTKDLCELLNCGTQKVTKIKKELENFNLLEQERMGLN
                     KPNKIYILEPKKPSKTSNNKEFRKSKFQNSENQNSRVLKIKKQEFRKSKSNDTNLSNTD
                     YIKTKNNDMNDLNDIAAKNKISNHSNHTNQFSNNLDDKDYKEILLQEFPDQLTNYLLNY
                     NYRDLEMIKAIILKAKKSFNSNHDDAYYMLEDIEDEILIALKRFKKAIYDRGVKGQKET
                     IHSMQGYLMQTILTELEELYSADMRRKNMTGNNIFNI"
                     /name="replication initiator protein A"
     ARG             7490..8226
                     /name="ErmB"
                     /gene="ErmB"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="98.78"
                     /coverage="98.79"
ORIGIN
        1 caagaaagtc gtcatgaaac aacaattgaa ttttacagaa aagagttgca agaaaggtca
       61 aaattgcttg aaaatcaaca aatattagca ttagaaagta ataaaaaaat tcaaaagtta
      121 gagaatcaat tagaagaaga aagacagctt aattactctt ttgataaagt gactaatgat
      181 agaaaaaata ttaatgcaca agaagccgat tttactgtag aacctaaaaa tactaaccaa
      241 caacaagagg aaaatcaaac aacggattat agtgttatat ctggtgagaa taaggatgaa
      301 gatataaaaa aagttactac agaggaagag aaacgagtta aagaacaaga aagtgagact
      361 aataatgagg tggagcagcc atctaaaaaa ggattttgga gtcgtttgtt tggtaactaa
      421 ttatattaaa aagcacgaca agttcatgaa cttgtcgtgc tttttatgtg gatagaactt
      481 ctgataatca tcgattatgt aaatgattgg tataagataa ataattatat atatcttata
      541 aaatataata gcaattatgg tgttgagcat cattactcac catatataag ctttcttata
      601 tgttattatt taattatatt aaagattgat agggtggagc gattaaattg ggataccaaa
      661 gcgaatatgg tttagaagaa aatgtgatta aacagctaca agatttgggg tatgagcgtg
      721 tattattgcg caacgaaaca caattaaaag agaattttag acgtatttta aatgaacgaa
      781 atgcagataa attagagggt acgcctatat cagatagtga gtttaaacgt atcatgattg
      841 atatcagtga taaaagtgta tttgaaagtg ctcaaatatt aagagataaa tatgtactag
      901 aacgtgacga tgaaacaaaa gtttatctta gcttaatgaa tataaaaaag tggtgtcaaa
      961 atacatttca agtcacaaat caagtaagtg tgaatgatac acacaagagt cgttatgatg
     1021 taactgtttt aattaatgga ttacctttgg ttcaaattga acttaaacgt agtggcgttg
     1081 cgattacaga agcttttaat cagattgaac gttatcgccg tcaaaactat acagggctat
     1141 tcagattcat acaaatgttt gttgtgagta ataaaatgga aacccgctat tatgcgaata
     1201 gcgatcaaaa aatcatgaaa agtcatatgt tctactggag cgatgaaaaa aatgagcgca
     1261 ttaatgtgct taaagatttt attgaatctt tcttagaacc gtgccatatt gctaaaatga
     1321 ttagccgtta tatgattatt aatgaaacag ataaaatatt aatggccttg agaccgtatc
     1381 aagtttatgc tgtagaggct ttgttaaatc gagctataga aacaaataac aatggttata
     1441 tttggcatac aacagggagt ggtaaaaccc tcacatcgtt taaagccagt caaatattag
     1501 cgcaagaacc agatattaag aaagttatat tcttagtcga tcgcaaagac ctagatggtc
     1561 aaactttagg ggaatttaat aagtttgagt cagattctgt tgataggacg ttcaatacta
     1621 aaaagttact caaacaaatg gatgatccta cacggaaact aattgttacg acgattcaaa
     1681 aaatggataa tgcgattaaa tcaggacatc cagcaatgga acgttataaa gaagataaag
     1741 tcgttttcat tattgacgaa tgccatcgaa cacaatttgg ttcaatgcac cgtatcatca
     1801 gacaacattt cggaaacgcc caatactttg gttttacggg aacacctaga tttgaagaga
     1861 atgcgagtca agatggacgc gcgactgcgg atatttttgg agaatgtcta caccattatt
     1921 taatcaaaga tgcgattaga gatggtaacg tgctaggttt ttctgttgaa tatatgaata
     1981 cgtttgaccg tagtgaaaaa attatcgaag atggttatgt taataaaatt aatgaagctg
     2041 aaatatggat ggcagacgaa cgtgttcaaa aagtagcaga acatattatt gcgaatcata
     2101 ataaaaaaac acgtgatcaa atgtacacag gtatgttgac cgttcaaagt attccaatgg
     2161 cgattcaata ttatcgtgtt ttccaaaaat tgaagaaaca aggtgtacat aaccttaatg
     2221 tcacgacaat attcagttat caagataacg aagccataga tgatacgatg gatgaacaaa
     2281 caagaagaga tatattagaa ggtatcattc aaacgtataa tgagaccttt gataaaaatt
     2341 tctcaacgga tacgtttgat ggttacttca atgatgtatc aaaccgtgtc aaaaaaggat
     2401 tacccaatca aaaattagat attcttattg ttgtcgatat gttcttaaca ggctttgata
     2461 gtaaaaaatt gaatacgtta tatgttgatc gtaatttaca gcatcatacg cttattcaag
     2521 cttactcacg taccaatcgt gtggaagagc caacgaaacc ctatggaaat attgttgctt
     2581 atagaaactt aaaagaaaaa acggatgagg ctattgagct tttctctaaa acagacaata
     2641 cagatacggt attgagctta tcgttccaag cgtacttaac gaaatttaaa caggctctga
     2701 ttgaactatt tgaattagct gaaacacctc aagccgtaga ccaattagaa agagaagaag
     2761 atcagcgtga ttttgtaatc gcatatcgtg agttagccaa gcatatgcaa aagttaaaaa
     2821 cgtttgatga atttgtattt gacaaggaaa cgttaggtat tgatgaacaa acatatgaag
     2881 attataaagg taaatattta gatatctatg ataaagtgat gcgacctgat aaagaaactc
     2941 tagagggaga atctatatta gacaatattg attttaatat tgagatttta cgaaatgatt
     3001 tgattaatgt tcaatatatt ttagatttat taaatcaaat tgatttaagt aatacagaac
     3061 aacaagaaaa agatattaaa gagattcgta aagtgcttga taaagctgat gatgatcaat
     3121 taaggttgaa atcagattta attcgaagct tcttagataa agtcgtaccg aacttaaatg
     3181 aaggtgattc aattgatgat gcttattata attatgaatc taaagtgaag gtcggagagc
     3241 tggaacaatt ttctgaggaa acagattatc cgttagaatt attgaaatcg ttagttaagg
     3301 aatttgaatt tagtggtcat ttaaataata acttaattga agaaggcgtt tctgggggtc
     3361 ttttgatacg tactaaaaaa ataaagcgtg ttaaatcatt tatcaaagag atggttaaaa
     3421 aatatagtgt ttaatgtaaa acaagtgtag ttttttaaaa ctacacttgt ttttatttta
     3481 aagtcatgct acatttgttt tgtagaaaaa caaaactaca ggaggtttgg tatgttaaaa
     3541 gattatgtta attatcaaag tggcacatta atacagcgct tagaagttga tgagagcgac
     3601 ctggggaatc gttatatgat atatgatcaa aatatgttag atatagattt agggttaacc
     3661 atagatgata catatcaacc tagagtcatc aaattaaaag atacatcaag agcaaccatt
     3721 gtacataaag atgatattgt tataagtatg atgacaggtg agtgcacctt agtgagcaca
     3781 aaacacgatg gttcaatttt accttataac tatacaaaaa ttgaagttac ttcagactta
     3841 ttggaacctg cttttttagt ctattggttt caactagcac cagaggtaca atcacaatat
     3901 aagcaatata tgcagggtgg ctcaacaata aaaaaactaa cacatcaaca gttaaaatca
     3961 ctctatatta ccttaccttc aattgaacga caacggttga ttggacaaat tggtattaaa
     4021 gaaaaacaat taaacgtatt aaaacaaaga caaagtcgat taaaaaagca attcttatcc
     4081 aacactttat tcaaggagga caattaaatg tctattacgg aaaaacaacg ccaacaacaa
     4141 gctgaattac aaaaaaatct atggtctatc gctgatgact tacgtggcaa tatggatgcc
     4201 aacgagttta aaaactatat cttaggtatg attttttatc gtttcttaag tgaaaaaatt
     4261 gaagaacaag cacaaatact cttagctgaa gacaatatag attatgaaac tgcgatggca
     4321 gatgaagatt atagacccgt cttagaacaa gaatttattt cacgtatcgg ttatgtgatt
     4381 gaaccacaat atctatttgg tcacttagtt aaaaaaattg agaaacaagc ttttgaaatg
     4441 gaagacctga gcaatgcgat taaaaacata gaaaattcta cacgaggtca tgatagtgaa
     4501 gatgacttta ttcacttatt tgatgattta gaccttaatt cttcacgttt gggtaactct
     4561 aatgctgcac gtacaaaatt gatttcaaaa gtaatgatga agatatctac attacctttt
     4621 gttcatagcg atatggagat tgatatgtta ggtgatgctt acgagtactt aattggtcaa
     4681 ttcgcagcaa gctcgggtaa aaaagctggc gaattctata caccacaaca agtttcaaca
     4741 attttagcta aaattgtaac cgcaaataag aaagatatta agagcgttta tgaccctaca
     4801 tgtggatcag gatcattact ccttcgtgtt gggcgtgaag ctaatgtacg tcaatattat
     4861 ggtcaagagt acaatagtac aacgtataac ttagctcgta tgaacatgtt attacatgac
     4921 attaactatg cgaattttaa gattgaaaat ggagatacga tagaagatcc ggctatttca
     4981 gacgaacgtt ttgaagctgt tgttgcaaat ccaccttata gtgcgaaatg gagttctgat
     5041 ccacaattct tagaagatcc acgctttagt aattatggta aattagcgcc taaatcaaaa
     5101 gctgactttg cattcattca acatatgatt tatcacttag atgataatgg aacaatggca
     5161 gtggttctcc ctcatggtgt gttattccgt ggtgcggctg aaggtgtcat tcgcgaatac
     5221 ttaattaaag aaaaaaacta tttagatgcg gtcattggtt tgccatcaaa cttattcttc
     5281 ggtacatcta taccaacaag tatacttgtt tttaaaaaat gtcgtgaaga tgatgacaac
     5341 gttctgttta ttgatgcatc gcaatcattt gaaaaaggca aaaatcaaaa ccatttaaca
     5401 tcagaagatg tagaaaaaat tgttgaaacg tataaaaacc gcgaaacatt agataaatat
     5461 agttatgcgg caagtttaga agaaatcgca gaaaatgact ataaccttaa tatcccaaga
     5521 tatgtcgata gatttgaaga agaagaacct attgatttag aacaagttca aaaagactta
     5581 aatcaaattg atgatgaaat tgtcaaagtc gaacaagaaa tagaaggcta cttaaaagaa
     5641 ttaggagtgt tgaagcatga ctaatcaaac aaaaaatgta ccagagttga gattcccaga
     5701 gtttgatggg gagtgggaag agagaaagat aggaaattat attgtagaaa taaaaaagta
     5761 tgacacacaa caacactcta gttattcagt tgcaacatca tcaagaagag gtctttttaa
     5821 acaagctgaa tactttgatg gtaatagagt ttttagtgat aaagaagttt tatatagtgt
     5881 agtcccgcca aatacgatca cttataggca tatgagtgat gataatatat ttaaatttaa
     5941 tatcaattca tttaacgaaa gtgtcttagt aagcagagag tatccagttt ttgacgttaa
     6001 tgattatgct gataagaact ttgtatttta tgaactaaat aatagtagta actttttaaa
     6061 attttgtata aagcaaaaaa agggaggtac tcgaacacgt ctatatttca aggtattaga
     6121 aaattacgaa acattttatc ctgaaaaaac tgaacaacaa aaaatcggtg aatttttcag
     6181 caaactcgac cgccaaattg aactagaaga acaaaaacta gaaaaactgg aacaacagaa
     6241 aaaagggtat atgcaaaaga ttttttctca agaattaaga tttaaagata aaaatggaaa
     6301 tgaatatcca gaatgggaag taataaaatt aaaggatatt ttaagtgaaa gaaaagaata
     6361 tgctagtaaa ataggaaatt acccacatgc tacattatca actagtggta tttcattaaa
     6421 gtctgataga tataacagag actttttagt aaaagataaa aacaaaaaat ataaagttac
     6481 tataatgaat gatatttgct ataatcctgc aaatttaaaa tttggggtaa ttactagaaa
     6541 tcatattggt tcagcaattt tttcacctat ctatattaca tttgaggtaa ataatgcaca
     6601 ttcaccatta tttattgaat tactagttac tcgaaatgat tttataaacc gagttagaaa
     6661 atatgaacaa ggcacagtat atgagagaat ggcagttaaa ccagaagact ttttaaatta
     6721 tgaaactaaa attccttgtt tagaagaaca agaaaaaatt ggtgatttct ttagtaaatt
     6781 agataaagta attaataagc aaaggcaaaa aattgatgag ttgaaattac gtaaacaagg
     6841 actcttacaa aaaatgttcg tataggggtc ccgagcacct acgaggaatt tgtatcgata
     6901 agaaatagat ttaaaaattt cgctgttatt ttgtacattt aacttgacgg tgacatctct
     6961 ctattgtgag ttattagtgg tacagttttc aaccgtttta attataaaaa agtggtgcat
     7021 ttttaaattg gcacaaacag gtaacggtta ttgcaggtgt atttcttatc tatgggttta
     7081 acatggattt tatcattaaa atcatgagta ttgtccgaga gtgattggtc ttgcgtatgg
     7141 ttaaccctaa agttatggaa ataagactta gaagcaaact taagagtgtg ttgatagtgc
     7201 attatcttaa aattgtgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa
     7261 ttaagaagga gggattcgtc atgttggtat tccaaatgcg taatgtagat aaaacatcta
     7321 ctgttttgaa acagactaaa aacagtgatt acgcagataa ataaatacgt tagattaatt
     7381 cctaccagtg actaatctta tgacttttta aacagataac taaaattaca aacaaatcgt
     7441 ttaacttctg tatttattta tagatgtaat cacttcagga gtgattacat gaacaaaaat
     7501 ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa
     7561 ttgaatttaa aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg
     7621 acgaaactgg ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc
     7681 aacttatcgt cagaaaaatt aaaactgaat actcgtgtca ctttaattca ccaagatatt
     7741 ctacagtttc aattccctaa caaacagagg tataaaattg ttgggagtat tccttaccat
     7801 ttaagcacac aaattattaa aaaagtggtt tttgaaagcc atgcgtctga catctatctg
     7861 attgttgaag aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc
     7921 ttgcacactc aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct
     7981 aaaccaaaag taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat
     8041 aaatattgga agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa
     8101 ctgtttacta aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt
     8161 accgttactt atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg
     8221 aaataattct atgagtcgct tttgtaaatt tggaaagtta cacgttacta aagggaatgt
     8281 agataaatta ttaggtatac tactgacagc ttccaaggag ctaaagaggt ccctagcgcc
     8341 tacggggaat ttgtatcgat aaggaataga tttaaaaatt tcgctgttat tttgtacaat
     8401 aaggataaat ttgaatggta ccataaacga tcgtttttga gacaaaacct cctactacag
     8461 aattaggagg tttttcttac atacttcagt ttctaaactt gtgttataat caatgtgtca
     8521 taaatgtcaa aaaacacgag tttttagacg atgggagtag tgtttatttg catcgagaac
     8581 gagatttcat tagtaagtac ctataaaacg attaaaagag gtggtattca aatggctaaa
     8641 attggttatg cacgtgtatc aacacaagat caaagtttag acggacaaat agatacactt
     8701 gaagaatatg gttgtgaacg tatctttagt gagaaagcta gtggacgtaa aacaaaaaga
     8761 acggaacttg ataaatgttt agattattta cgtgaaggcg atgtgttggt tatttacaaa
     8821 ctcgatcgtc ttggtcgtac aacgaaacaa ctcatcgaac tttctcaatg gttggacgaa
     8881 aataacatcg atttacatat tattgatatg aacgtatcga ccaaagatgc gatgggaaaa
     8941 atgttcttta ccatgatgag tgcatttgcg gaacttgaag ctaacttatt aagtgaacgt
     9001 acaaaaaaag gactagcagc tgcaagagca agaggacgca aaggtggtcg gccttcatta
     9061 cctgaccaca agaaaagaga aataaagttc ttatatgatg aacaaaaatg gactggtgaa
     9121 gatatcgcaa agaagacagg tgtaagtcgt tctactgttt atagagtaat taaagaatct
     9181 tagttaacta tatggttctg ttaatttggt tatcatggag gttttataaa tactctaagt
     9241 ttattaaaat gatgaataac ttaaataaat ttggaataga aaaaccaccg ttaattttca
     9301 aactatataa ctttatattt tttaattaat aataaatact cttttgcatt cctaccctat
     9361 atgatgttat actgtattta acaaagagag ttccataggt acaccaatcc cctcactatt
     9421 tccggtagtg aggggatttt ttggtgcagc tatgtcgcct tattacttgt tacgattgtt
     9481 tagccaaaac gtgaaaatcg caacaatgca tcctgaaatc acaggtgcaa ttatactaac
     9541 aaagagaatt gccataggtc tcacctcctt ccatagtaat ttcgcctatg gaagttaagg
     9601 cgactccatt attatatcat ctgtgtcttt taaagcatat gcacttccga taagttgaat
     9661 tatgttattt tctatacggt attttccttt taccaaacca taatgaatat aatttataac
     9721 cacctactat aatgtatgta gtgaataata tagaaggaca tgtcattaat aatttaaatt
     9781 ttattaatga tatctctaaa ttggtagacc aaagtgtaaa tattgaagct atagataacc
     9841 ataaaatata gatagctaat aaaaataata cactataaat tactcttctc cagattaaag
     9901 gaagtatttc gaattcttta aaattatttg tgctgatatc aaaaacccat ttgtattttg
     9961 gtataaatac caacctacct tccctacttt ttatatcttc cactgtttcc tcgtatattt
    10021 ttccatcttc atcttcataa attaaattaa attatcaatt ccgttataat tcaatcgctt
    10081 tgaaagttta aaatactttt tttctccatc tttattaatt aactctaaaa gaaaaaattt
    10141 ccttttctta atccaaatgg gtgaaagtaa cagtgcgaaa tacatagaat acataaataa
    10201 aaatattaag tatccataaa aagtttgctg atgttctttc atttctttta tactaatttg
    10261 agtaattact tcagataatg ttaaaagaat taacatgcaa atcatgattg aaaccattat
    10321 aaatataaaa aaatttgaaa gaattgataa agctttagta cttttttgat tcatttcaac
    10381 atggtgtaac tttggaaatg agattttttt tattactatc gatccaaaca caacgattaa
    10441 aatttgccaa aataataaat cccgagtaaa taactctaaa aactccatga taatgcctcc
    10501 aataaaaaaa ttaatacata actattatat ttcactatat atatgtataa ttttcgataa
    10561 taaacggact aattgcaaca ttaaaagcag aaacacatga tggtgaatta ttaagaagaa
    10621 tattcaataa ttagtatcaa aataaaacac tgatctatta tgattggtgg tttatttttt
    10681 gtttaatcaa cacaaaaaaa agaatttata aggctttttt tagaatttaa aagaatttat
    10741 aaacgtataa acgttgattt aataggcttg taaagaattt agatacaagt tcactcaatg
    10801 ccgctgcaca aaaaatgtaa aaatgcacat ttgttttttg ttaaattttg taatctcgta
    10861 aaccattgat ataataggtt ttataagaca tttacttttg agcataagac tgtttgtaac
    10921 gggctagata cctcagctat gctgagtgac acgctagtgg cattgtatcc ccttatgctc
    10981 ttaggttaga atctcaaaag aagttataag agaagataac ctaatataat tagaatgaat
    11041 caggataaaa attaatttga ttcgtctcat ttaaaccata tattaaagtg attaattttt
    11101 aatattttaa aattattcca cggcgtttaa ggatctatta ataaattaat ccttcaataa
    11161 ctctaaagaa aatattgcac aacgagtaac tttagctata attttttcta ataataattc
    11221 cccatttcat tttttgtttt atgatttatt ggatcaattt aatttaaaaa taatcattaa
    11281 taatttgaaa gcacaaatta attatccctc attcacaaat tgaatcttta aaatacaaag
    11341 tctatatcca tgatttttca aaatcatttg tatatctttt atcagaaatt caagtttaaa
    11401 atttactctt ccccaattca tttcctgttc aatttgtaat atataaaaat gttaatcagg
    11461 ttttttacac agatattatt tcaccattaa aaacaaataa tgacttttta tcctaaaaat
    11521 tttattattc ttgtcataat taaattgcta ttaatatcaa agcaatgaaa atcatttcaa
    11581 aaaatattcc tttttcagaa gaaaaaggaa tcaaacaata aaattattct acatcttcta
    11641 aaggtaagct catgcctttc caaccttcta aagctccagc taattcatat gcttcaaaac
    11701 catgcttgta aaaatctaat aaagcagcct tacctaaaat agttccacca gtccaatcat
    11761 aaactacata tatcttatct ttgtctaaac tagataccaa taattagcta aaaaacttat
    11821 tactgagcga gtttccataa aataatcatc ttttaaacat atttaagaag tatatataaa
    11881 cgatcattat ttgatcgttt atatatctag aaatggattt tctttatttt gtactgtttt
    11941 tcttgcttgc tctgtaaaat gaaattcttc taaaccattt actaatgaat tatagtagta
    12001 gcctaacatt gcttgaactg attcgcgttt ctttttcatt tctcgattga cagttattaa
    12061 aatatccata attttttccg ataagaatgg gttttcttcg aacataaaga ataaactatc
    12121 accacgtcta ttagctgctt cccttttagc ttgcataatt ttatttataa tacttatgct
    12181 atcagctaat atatatctac ttttaatgaa ttcttgtaag tctttaggta agttttttaa
    12241 taaatgagat tgttcatcaa ttaaataatt atcattttca tggtttgaat ggagtgaatg
    12301 gtcatttatt tcaggcatac tatctatatt attaccactc attccattca tttcactaat
    12361 atcagtatta ctaatatcag tattactaat atcagtatca tttgcgtatg attttgatac
    12421 gtcttgatgt atgtttttca tatttcttga cgtatgattt tgatacgtct ggacgtatga
    12481 ttttgatacg tccttgtcat tactgacgtc atcgccattt tcttcgctaa ttatagtata
    12541 aatatcatct tgtgtaacaa ctggcttttt aagatataat ttattaggtt tatttaaacc
    12601 aactcttatt tgactaaata aatctgcttt ttctagctct ttttattttt gttaattttt
    12661 cttttctaca atttaagatt tccattagct tttcatttga ataaatacta acaaagaaaa
    12721 aagcagataa aacgaacatt attgtcacat tcaataaatt ttctcggcat aaatgcgtcg
    12781 tctacttttt tctagaacga ttaatcaaga aataacctaa aaggccacaa atcaaaataa
    12841 ataaaaatat aagcattttc tatctccttt ttggcaaaac tttatcacaa gacttattaa
    12901 aaaattaaat acccttattt tggattttaa gggggcattt taaagattta ggggtaaaac
    12961 atatagtttt atacctaaaa acctacataa gcttttaaaa cgcaaatatg agccgaataa
    13021 atatattcta attctacaaa ctaaaatttg atcaaaacca gtgactaatt ttttagacac
    13081 tgcccattta catgcaaatt taaaaattgg cataaaaact tgtcaaccat gccggttgaa
    13141 cgctatagtt cctgcagagg caaaaggcat aaaaaaaacg ctagcttttg agctaacgtt
    13201 cattatttcg ttttataatt tgagtcagtt ttgcaatagc tattaaaacc aacattggta
    13261 taactccaaa gataaatgac atccacatat gacaaaactc cttatcaaca gttaaaaatt
    13321 cggactttga ttgaatattt ttaagtttct ttgggtaatg taagtacatt gaaattaaat
    13381 attaaaaata ttgttgcctg tcatattttt tcttctcata tctgctgaat ataattcttc
    13441 gagttcagtt aaaattgttt gcattaagta tccttgcatt gaatggattg tttccttttg
    13501 acctttaact cctctgtcgt atattgcttt tttaaatctc tttaaagcaa ttaatatttc
    13561 atcttcaata tcttctaaca tataatacgc atcatcgtga tttgagttaa acgatttttt
    13621 ggctttaaga ataatagctt ttatcatttc taaatctcta taattataat ttaataaata
    13681 attcgttaat tgatctggga attcttgtaa tagaatttct ttataatctt tatcatccaa
    13741 attatttgaa aattgatttg tgtgatttga atgattgctt attttatttt tagccgctat
    13801 atcattcaag tcattcatat cattattctt agtctttata taatcagtat tacttaagtt
    13861 agtatcatta gattttgatt ttcgaaattc ttgttttttg attttcaaaa ctcttgaatt
    13921 ttgattttca ctattctgga attttgattt tcgaaattcc ttgttgttac tggttttgga
    13981 aggctttttt ggttctaata tatatatctt attaggtttg tttaaaccca ttctttcttg
    14041 ttctaataaa ttaaaatttt ctaattcctt tttaatttta gttacttttt gagttccgca
    14101 atttaaaagt tcacataaat ctttagtagt aaatattaaa tatacattcc cattttcatc
    14161 aatccagtta ttcattttag ataattcaaa acgatctcta aatatacagt aggcaatttt
    14221 tgaagaatca ctaagtttcc taaaatattc atcttcaaat aaaaatttat aaagtttata
    14281 aaatctttct tgttcaaatt cttgtattgt aattcttttg aaatttgaca taataaaaac
    14341 ctctttccga attttagtat ggtaagaggt aataaaacgc ttttatatag ctttacattt
    14401 atttaacgat ttaagttctt gcactaaggt gacattcatt atataataca agtataaaat
    14461 tgatattaaa caaacgtgcc gcaacgtttt attaccaacc cccaactatt accagtagtt
    14521 gggggtttta atgtgttaag actattatag cactttaaac aaggttataa aaggagaaaa
    14581 taagagtttg tcaagggttg ataaaggctt gtcaacaact ataaaccctt gacaaacctt
    14641 tgacgaaata agatcatttc atcttttata actttgaata taaaccttca taagtttata
    14701 atatagcata aagggttgat aaacatttta taaagggttg attatatgaa aagtgtgaaa
    14761 atcttaagtg aagaattagg ggttagtaag caaacaattt ttaataacat taaacgattg
    14821 aatatagaga cattgaaaca ggaaaatact tcctttatta aagaagatac ggatatagaa
    14881 aaaattattc aaagggtaaa tgaaaataaa aagaaatatg gttttgaaag tactgcagaa
    14941 gacaaacaaa aaaaaaagtc agataataat gttgaatcca aaagtaatgc tcaaatagta
    15001 gaaatcctta aaaaacagat tgatacttta aatgatcaaa ttgaaaga
//
