LOCUS       NZ_CP076743             8964 bp    DNA     circular CON 16-APR-2024
DEFINITION  Acinetobacter baumannii strain X4-705 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP076743
VERSION     NZ_CP076743.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19690117
            Assembly: GCF_029774355.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Hsieh,Y.C., Wu,J.W., Chen,Y.Y., Quyen,T.L.T., Liao,W.C., Li,S.W.,
            Chen,Y.C. and Pan,Y.J.
  TITLE     An Outbreak of tet(X6)-Carrying Tigecycline-Resistant Acinetobacter
            baumannii Isolates with a New Capsular Type at a Hospital in Taiwan
  JOURNAL   Antibiotics (Basel) 10 (10), 1239 (2021)
   PUBMED   34680819
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   Pan,Y.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) Department of Microbiology and Immunology,
            China Medical University, Taichung, Taiwan, No.91 Hsueh-Shih Road,
            Taichung 40402, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 374x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029774355.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 10:13:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,957
            CDSs (total)                      :: 3,861
            Genes (coding)                    :: 3,781
            CDSs (with protein)               :: 3,781
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 28 of 80
            Pseudo Genes (incomplete)         :: 46 of 80
            Pseudo Genes (internal stop)      :: 18 of 80
            Pseudo Genes (multiple problems)  :: 11 of 80
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076743.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="X4-705"
                     /isolation_source="pleural effusion"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed1"
                     /geo_loc_name="Taiwan"
                     /collection_date="2020-07-10"
     gene            join(6827..8964,1..268)
                     /gene="znuD2"
                     /locus_tag="KRO89_RS19170"
                     /old_locus_tag="KRO89_19055"
     CDS             join(6827..8964,1..268)
                     /gene="znuD2"
                     /locus_tag="KRO89_RS19170"
                     /old_locus_tag="KRO89_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_279447081.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLDFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTLAY
                     KNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            573..1034
                     /locus_tag="KRO89_RS19175"
                     /old_locus_tag="KRO89_19060"
     CDS             573..1034
                     /locus_tag="KRO89_RS19175"
                     /old_locus_tag="KRO89_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(1357..1530)
                     /locus_tag="KRO89_RS19180"
                     /old_locus_tag="KRO89_19065"
     CDS             complement(1357..1530)
                     /locus_tag="KRO89_RS19180"
                     /old_locus_tag="KRO89_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(1530..1901)
                     /locus_tag="KRO89_RS19185"
                     /old_locus_tag="KRO89_19070"
     CDS             complement(1530..1901)
                     /locus_tag="KRO89_RS19185"
                     /old_locus_tag="KRO89_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(2173..2427)
                     /locus_tag="KRO89_RS19850"
     CDS             complement(2173..2427)
                     /locus_tag="KRO89_RS19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            3111..4061
                     /gene="repM"
                     /locus_tag="KRO89_RS19190"
                     /old_locus_tag="KRO89_19075"
     CDS             3111..4061
                     /gene="repM"
                     /locus_tag="KRO89_RS19190"
                     /old_locus_tag="KRO89_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            4054..4629
                     /locus_tag="KRO89_RS19195"
                     /old_locus_tag="KRO89_19080"
     CDS             4054..4629
                     /locus_tag="KRO89_RS19195"
                     /old_locus_tag="KRO89_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            4649..4792
                     /locus_tag="KRO89_RS19200"
                     /old_locus_tag="KRO89_19085"
     CDS             4649..4792
                     /locus_tag="KRO89_RS19200"
                     /old_locus_tag="KRO89_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(4975..5802)
                     /locus_tag="KRO89_RS19205"
                     /old_locus_tag="KRO89_19090"
     CDS             complement(4975..5802)
                     /locus_tag="KRO89_RS19205"
                     /old_locus_tag="KRO89_19090"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            6111..6398
                     /locus_tag="KRO89_RS19210"
                     /old_locus_tag="KRO89_19095"
     CDS             6111..6398
                     /locus_tag="KRO89_RS19210"
                     /old_locus_tag="KRO89_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            6385..6699
                     /locus_tag="KRO89_RS19215"
                     /old_locus_tag="KRO89_19100"
     CDS             6385..6699
                     /locus_tag="KRO89_RS19215"
                     /old_locus_tag="KRO89_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     ARG             complement(4979..5802)
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          3112..4061
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgattttgat gaatcgctta aaggtgaaat agaatattat cgagtcttta aacaagataa
       61 tatttctaaa tttgagcagg ttacatcagg ttataatatg cttaatatga ctttagcata
      121 taaaaataag ctatctcaca cagaatatga tttattcttt aaagcgaata atttacttga
      181 tcagaaagtc tatgcccatg aaacattttt gccatatatt ccccaaattg gaagaaattt
      241 tagtttaggc ttaaatctta atttctagat aagatactgt aatcttctaa gaaaatttct
      301 tagaagattt ttcttataaa atgattagat taataattaa ggtaagcgtc cgctgaatcc
      361 catacctatt ttttaattcg atttaggttt ccagcagtgt ggcagtaact cttcaatctg
      421 ggtcacttta tgtgtcggca gccttttcag cactgaatag ccaccgattt tgtagacacc
      481 ttttagctag ataaaaagca atttttttga tctttaaaga atgaggatat aagctgttta
      541 aaacttaatt ctaattggtt aataggtgaa aaatgaaatt agcagaagct ctcttacttc
      601 gaagtgatca acaaaaaaaa attatttcac taaaacaacg tatcaatgca aatgtattgg
      661 tacaagatgg tgatcaacca tctgaagatc caaatgaact actaaaacaa gtattttctt
      721 taattcaaga atttcaaaaa ttaagttatg caattcatga aactaatgct ttaacaaaat
      781 taaatgatgg ccgttctcta cttgctttat tgactttgcg tgatgaattt gtagaacagc
      841 acaaaaccct aactgcagca ataagtaata catctcgtga atctgatcgt tatagtacgc
      901 gggaaataaa gtggcataaa gttattccag tttcatcatt acaaaaacaa gctgatgata
      961 ttagtcttaa attacgtgat ttaaacgtgt taattcagtc gactaattgg aaaattgatt
     1021 tgattgaagc ctaatagtcc tttagatata atcaagttga acatactggg cgagtacaag
     1081 atcccttact tccacaacat aggattgtaa ataaagtgta gtggcttgca aagcaagtgg
     1141 ataagcttaa aattttaagt acaaatcaat ccatcacttg gcataactca aatctttgtg
     1201 caaacaaagt tacacgttac ttataactac catgtactga cagtatgttc acctcaatta
     1261 ctaaaaataa agaaatgaac aagcatcagc tcaactacat ttctattttt aattcagttc
     1321 ggcatttaac ataaaatctc ttatacgaag cccaggttaa ttctgggctt tttgttcaat
     1381 ctcatcattg gccgagaaca tacttggatt cgtaagcctg atattttcct gacaacttcc
     1441 acacagcatc aatcctgcaa aaagttgtac attttgtcta ttttcacaaa gcgtacatgg
     1501 ctttgaaagc tgaataatat tatctgacac tactttaccc tcttatagtt atcatctgca
     1561 tatgaataac ctttagcttg tatcattcta acctcatctt tagaagcgcc atttttcatt
     1621 aagagccaca acatcatgaa atatagtcca gaaaagtctt catagtattt ttcagaaaaa
     1681 ttgttatctt cctcaaaatc atctattcgc ttttctaatc gattcaccag atcctcaata
     1741 cgatcggctt gctgtttaac agcaatagat tgaaaatcat tggtcaaaat tttactgaat
     1801 aatttactgg ttctgattcc ttctgaatta gccaattgat cgtagaattc tgctacttgt
     1861 ggatctaacc tgactgcata agttttgact ttttcgccca taaattacac ctcttattaa
     1921 aattagattt tgtacaaaat taaatatata taattcaact aattaaataa aaaataataa
     1981 ttagattacg tacaaactta tttgtacaga attaatttat tctaaaacaa aaacttattt
     2041 aaatataatg ctttttttat gtacaatcat gagtacatat tttttcttaa atttatcaat
     2101 atcttatcta aaaaaagtgt tgttttgatg tacaaccatg agcacagcat aaaaaataga
     2161 taattcaatc acttattcta aaaaaagacc atttttctgt acacgtttct gagaccctaa
     2221 aaaatcgctg aaatccttat cctgtaaggc tttgaggggt gtcatcgtta gatgcgtggt
     2281 gtgtgacaaa aaaatcccgg catgtgccgg atcttggatt ggaaaattgg ctaaagtgac
     2341 gtagggccgg tgcttggttt tgcatggaaa aaagtattta tttccttgtt tagaaaaacg
     2401 taaaaaatca attttttgtc cattcatcca ggttaaaaat ttcaacccaa aactttaatt
     2461 atgaaaagct tcatagaacg cgttcaaatg cgatttaaga gcctttacct agaaaacata
     2521 gatctcatag tgaaaaacac aaaacagctc aaaaaacgca aatgagagcg aaataaagag
     2581 gtattttgac tttagataga tgcatagagc gagtgtctac gagcgaacta tcaaaatttg
     2641 cgcctagacc ctctgaaaaa catttttttg tcctctttag cctaagaaag cgtaagtttc
     2701 gtgcagaaat ttgctcctgg atcgagcgta gcgagtaaaa aagttttatg agcgaagcga
     2761 attccaagtt tcttttgctt ttttttaaag tcacgccagt attaaccaaa aaattgcccc
     2821 aacgaactga gcgaaagcga agttcaatag agtttgagcg aagcgaaaac caagggcaat
     2881 ttttcattcc ctgggctttt aattatttta aagtttttaa atacttttaa atggcttgaa
     2941 agccttatga ataaagggtt ttagctctta tatgtccacg tttaccttgc aatatgtcca
     3001 cgtttacctt gcaatatgtc cacgtttacc ttgcaatatg tccacgttta ccttgcatta
     3061 gtacacaaat aatattaacg tgtacttata cacaataaaa aatagtggct atgagagatt
     3121 tagttgtaaa ggacaatgcc ttaatcaacg caagctataa cttagactta gtagaacaac
     3181 gtttaatttt attggctatt gttgaagcaa gggaaagtgg gaaagggatt aatgcaaatg
     3241 atccattaga agttcatgca gatagttata tcaatcaatt tggtgtacac cgtaatacag
     3301 cttatcaagc cttaaaagat gcttgtaagg atttattcgc gcgtcaattt agctatcaag
     3361 agaaaaaagc taatgggaat atccgaaatg ttatgagtcg ttgggtatct caaattgctt
     3421 ataacgacaa tgaagcaact gtagacttaa tatttgcacc tgctgttgtt cccttcataa
     3481 cccgactgga agaacaattt actaaatatg aattacagca agttagtagt cttagtagcg
     3541 cttatgccat tcgcttatat gagcttctaa ttcagtggcg aagcactggt aaaaccccaa
     3601 ccatagaact acaagaattt agaaagaagt taggcgttct tgataatgaa tatttacgga
     3661 tggctcattt aaaagagcgt gttttagagc tttcaattaa acaaataaat gagcatacgg
     3721 atataactgt aaaatatgaa cagcataaaa gaggacgttc tatttcagga ttttctttta
     3781 cctttaaaca gaagaagaag gatagcccat caatagaaag agatccgaac actttggagc
     3841 ttttttcaaa gatgaccgat gctcaacggc atatgtttgc aaataaactt tcagaactcc
     3901 ctgaaatggg tcgctattca caaggaacag aaagctacca acagtttgct gtacgtattg
     3961 ctgagatgct acaagatccc gctcaattca aagagctata cccataccta aaaaaagtgg
     4021 gatacatgcc atcaaataaa aaggacaccg taaatggcta agttatcact aagtgaagta
     4081 tctaaaaaat ttcatgtgga tagatcaacc atttacagag ctgtacgtaa tggacgttta
     4141 tcacgctcca gtgatggaca attcgatcta gcagaggtca tacgatgctt tggagaacct
     4201 gagcaaacat ctcaaaaaat tgaatcatct aagcaagaag gtgatgaatc tacaaaaaaa
     4261 cttattgccc atttagaaaa tgaagtcaaa aaataccaag aacgtgaaga acggttaatg
     4321 caacaaattg accgtatgca aacactcatt gagctgaaaa gtgttgcacc tgccacagca
     4381 gcaccacacc aagatgctac ggcatgcgac accaagatgc cacagcatgc gacaacacaa
     4441 caagacactg ataacaaaaa gaataatgaa ttaaatattg cagaaaatgt agcagtgcca
     4501 cagcaagaaa ctacggcata ccacacccaa acgctacagc atgccacgtt gcaaagtgtg
     4561 gcagtgccac aacacaaaaa acgtggttta tttggccgtg tgctgaatgc cgtctttgat
     4621 aatgactgag caataagggt gaaagatcat gccgaaactg aaagacattg ccctgggaat
     4681 tattgtagct ccgctgctga tcccgatcat gctgattgca tcgtaccagg ataaaaaggc
     4741 gctgaagaaa gagctggatg aatgccaaaa agaaaaagac caggcatcct gattattcaa
     4801 ttgagctaat caaaagtgtg gaacattgat gaaataatag cgtggaacgt gaaaagtcat
     4861 aaaaaaagcc cgaacaggga tgctcgggct ataaactaga aactacagcg ttgaattata
     4921 aggataatta tacagcattt cgtataaggc gtattatgtt aattttagaa attattaaat
     4981 gattccaaga ttttctagcg acttataagt aatttcatta cgaatagaac cagacattcc
     5041 ttctttcatt tctaagttga gcgaaaaggg gatttttttt ccattagctt gctccaccca
     5101 accagtcaac caacctacct gtggagtaac atccattccc catccacttt ttgcataaat
     5161 cttactacca tttacttctt taattagaag cattttttta acttcttctt gagtttctaa
     5221 tttaaaaggt aatcggttat gtgcaaggtc atcggcaaaa ttaacttctt gtactggtgt
     5281 aattttaagg gggccaacta accaaaaatt atcgacctgt gttccaatat ttgtatttcc
     5341 aaaattaacc cgctttactt ctttctgcat tagctctagg ccagtccgtc ttgcaagctc
     5401 ttgatatact ggaactgctg acaatgccat tgcctcacct aaagtcatat ctttctccca
     5461 cataggataa gttctttttt taccatccca tttgaaaatc tcatttgttg ttgctttatg
     5521 attttctagc ccgattaaag catttagcat cttaaatgtt gatgcaggga catattcttt
     5581 atttgctcgt gcaagagcat taccataggt gctaagattt ttaccctctt taataataat
     5641 tacaccctgt gtttgagctt catcaaaata gcttttaata gctttttcat gttgctgaga
     5701 agaaatatga aaattatctt cagatttagt tttaatagat gaacatgcac tgagagaaac
     5761 tagaatagaa atgctgaata taggaagtat aaattttttc attacaaatt catgttaggg
     5821 gaaattttgg ggcttagagt ataatatttt tattaaaaaa gttaaaactc ttttaattta
     5881 acataatgga cgttatgcga agtacacttg tatattaata taaatatcaa ctacttaata
     5941 aaaatagaaa aatcccaaaa tgcccaaaaa gccgactttg aaacaagtcg gcttttttat
     6001 gagcagtttt gatacttctt gccaataatt ttgactaaaa attacacgaa tctggttgag
     6061 tgatacaatt ttgtgataca ttattgtata tacaaatttg aatgtttttt atggaacagt
     6121 attttgaatg ggatgaggct aagaatcgaa agaatcagaa aaagcatgat atctctttcg
     6181 aaactgcaag ccttgttttt gaagatccat tacggatctc aatccaagac agacatacca
     6241 atggtgaaga acgttggcaa accattggac gagttaaagg cgtactaatg ttattggtgg
     6301 ctcacaccat ttttgatgaa gatgactgtg aaatcatccg aatcattagt gcaaggcaag
     6361 tcactaaagc ggagcgaaac aaatatgagc atggttagat actcacacaa agagctgaat
     6421 gagaaatttg gtgagaagca agatgctgaa attcaacgct tgcttgctaa aggcacggtt
     6481 cctgatgatc agctagatct atcagatatt cctgaaatta cagactggag caatgctgta
     6541 cgccaaaatc aattttaccg cccagtaaag caacaaactt caattcggtt agatgctgat
     6601 gtacttgctt ggttcaaagc acaaggcaaa ggttatcaaa cacgtatgaa tgaaatttta
     6661 cgagatgcta tgctaaaaga attaaaaaat catcaataag aatgggagct taggctccca
     6721 tttttgactt catttttagg ggggattacc attcaaaaat ttaaatagta gattttataa
     6781 atttttaagt gttatattat aatataacat taaatttggt tgttttatgc taaataaaag
     6841 taaactattt ttagctttaa ttactttagg ggcctcgaaa attttattgg cagctgaagg
     6901 gcctgttact accttaaata caatagtatt aactgcacaa tctgatgagt tagggtctga
     6961 gctgttaggc aaaagtctaa atgttagtaa tcagtttatt gatactagta aacttaaaca
     7021 gcgttcgaca actctagggg atgccttggg tacggaatta ggaattcatt ctaatcaata
     7081 tggaggtgga gcatctactc ctattattcg tgggcaggaa ggaaaacgta ttaaagtttt
     7141 acagaataat gctgacgtgt tggatatgtc taatatgtcc ccagatcacg ctgtcacagt
     7201 tgaaccaagt ttagctaagt ctattgaaat tatacgtggt gcaagtaccc ttttgtatag
     7261 ttcaaatagt gcggcagggg tagtcaatgt cattgactat aaaattccaa cccaaatgcc
     7321 tcaagacgga ttagagggta atactacatt acgttttaat actggcagta atgagaagtt
     7381 gaccacagca ggtgtaacag ttggcttaag cccacatgta gctttacggg cagaagggtt
     7441 atatcgtaat gctggtaact ataaaactcc tcattaccag tctagctctt ataattcttt
     7501 agaagatctt gaaaaccaaa acagtattta taaaaattta aaatatttac cagaaagctg
     7561 ggcagagtcg agagtaggta cgcttggatt atcctggata gacgataata cttatttagg
     7621 agtttcttat acacatcgtc atgatgaata tggtttacca gcccatagcc atttatatga
     7681 aggttgtggt gcctctgcaa tcggtatcga ttcacgaatt agtggattaa aaaattatct
     7741 tttatattat cctcaattga tggatgagca agatattaat tacataaatc cacgtcctga
     7801 ttgtcatcag cataatcata ttcatgaaac taatttctct cataatgctc cttatattga
     7861 tttaaatact cgacgttatg atgtgcgtgg agaattcact caaccattta ccggtattga
     7921 caaaattcgt acctcattga gttatataga ttattttcac aatgaactcg agggagataa
     7981 gattactaac ttttttaaaa atacaggaaa agtaggccgc atagagttat cacatcagcc
     8041 attaggggaa ttaaccggta tcttggggct acaatatttg gaacaggata atagtgcttt
     8101 atcaccagtc cattctcaag aaggccatac cacctatcta gataatcagc agctattaaa
     8161 tcgaaatgtc actaaaaact ttagtgtatt tggcctagaa aaatataact ggaacgactt
     8221 tactttcgaa ctaggtgctc gtatagaaaa gcagaaagtt agtatggatt atgatataga
     8281 aaaaattaaa gattcaatga aaccttggcc taataaatat aacagtcctt atgtagaaaa
     8341 aaataataaa atcagggctc aaaatttaaa atctatttta gaagctgttc aacctaacaa
     8401 agaaacagct ttttcttatg caggtacagt ccactggaga tttgctccaa attatatctt
     8461 atcattaaca ggtacacatc aagaaagatt acctaatgca caagaaatgt atactcatgg
     8521 tatgcatctc gctactaatt cttttgaaat aggtaaccgc tttctaagaa aagagaaatc
     8581 caataattta gaaatcagtc tggcctataa ggatgattta cttgattatc aaatcagtac
     8641 ttactattat gattttgata actatatata tttacagaca ctcaatgaag ttttaggaac
     8701 tacaaaagtt agagatcagc atacactacg tattaatcat tatagccagt ctgctgctaa
     8761 tttttatgga ttagagggca acatagggta tcaatttaat tctgtttatc acggctctct
     8821 ttttggagat tatgtgaaag gccgtttaac aaatttacct gatgctgtaa ttgcatacga
     8881 tatctggaat agagaaccga ctttagctcc ccaaaaggat cgttacactc ctagattacc
     8941 tcctgctcgt ttgggtacac gact
//
