LOCUS       NZ_CP076740             8954 bp    DNA     circular CON 16-APR-2024
DEFINITION  Acinetobacter baumannii strain X4-584 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP076740
VERSION     NZ_CP076740.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19690116
            Assembly: GCF_029774335.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Hsieh,Y.C., Wu,J.W., Chen,Y.Y., Quyen,T.L.T., Liao,W.C., Li,S.W.,
            Chen,Y.C. and Pan,Y.J.
  TITLE     An Outbreak of tet(X6)-Carrying Tigecycline-Resistant Acinetobacter
            baumannii Isolates with a New Capsular Type at a Hospital in Taiwan
  JOURNAL   Antibiotics (Basel) 10 (10), 1239 (2021)
   PUBMED   34680819
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8954)
  AUTHORS   Pan,Y.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) Department of Microbiology and Immunology,
            China Medical University, Taichung, Taiwan, No.91 Hsueh-Shih Road,
            Taichung 40402, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 262x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029774335.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 12:02:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,953
            CDSs (total)                      :: 3,857
            Genes (coding)                    :: 3,778
            CDSs (with protein)               :: 3,778
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 27 of 79
            Pseudo Genes (incomplete)         :: 46 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076740.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8954
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="X4-584"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed1"
                     /geo_loc_name="Taiwan"
                     /collection_date="2020-06-17"
     gene            579..1529
                     /gene="repM"
                     /locus_tag="KRO88_RS19150"
                     /old_locus_tag="KRO88_19035"
     CDS             579..1529
                     /gene="repM"
                     /locus_tag="KRO88_RS19150"
                     /old_locus_tag="KRO88_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            1522..2097
                     /locus_tag="KRO88_RS19155"
                     /old_locus_tag="KRO88_19040"
     CDS             1522..2097
                     /locus_tag="KRO88_RS19155"
                     /old_locus_tag="KRO88_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            2117..2260
                     /locus_tag="KRO88_RS19160"
                     /old_locus_tag="KRO88_19045"
     CDS             2117..2260
                     /locus_tag="KRO88_RS19160"
                     /old_locus_tag="KRO88_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(2443..3270)
                     /locus_tag="KRO88_RS19165"
                     /old_locus_tag="KRO88_19050"
     CDS             complement(2443..3270)
                     /locus_tag="KRO88_RS19165"
                     /old_locus_tag="KRO88_19050"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            3579..3866
                     /locus_tag="KRO88_RS19170"
                     /old_locus_tag="KRO88_19055"
     CDS             3579..3866
                     /locus_tag="KRO88_RS19170"
                     /old_locus_tag="KRO88_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            3853..4167
                     /locus_tag="KRO88_RS19175"
                     /old_locus_tag="KRO88_19060"
     CDS             3853..4167
                     /locus_tag="KRO88_RS19175"
                     /old_locus_tag="KRO88_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            4295..6706
                     /gene="znuD2"
                     /locus_tag="KRO88_RS19180"
                     /old_locus_tag="KRO88_19065"
     CDS             4295..6706
                     /gene="znuD2"
                     /locus_tag="KRO88_RS19180"
                     /old_locus_tag="KRO88_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            7011..7472
                     /locus_tag="KRO88_RS19185"
                     /old_locus_tag="KRO88_19070"
     CDS             7011..7472
                     /locus_tag="KRO88_RS19185"
                     /old_locus_tag="KRO88_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(7795..7968)
                     /locus_tag="KRO88_RS19190"
                     /old_locus_tag="KRO88_19075"
     CDS             complement(7795..7968)
                     /locus_tag="KRO88_RS19190"
                     /old_locus_tag="KRO88_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(7968..8339)
                     /locus_tag="KRO88_RS19195"
                     /old_locus_tag="KRO88_19080"
     CDS             complement(7968..8339)
                     /locus_tag="KRO88_RS19195"
                     /old_locus_tag="KRO88_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(8611..8865)
                     /locus_tag="KRO88_RS19835"
     CDS             complement(8611..8865)
                     /locus_tag="KRO88_RS19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     ARG             complement(2447..3270)
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          580..1529
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaacacaa aacagctcaa aaaacgcaaa tgagagcgaa ataaagaggt attttgactt
       61 tagatagatg catagagcga gtgtctacga gcgaactatc aaaatttgcg cctagaccct
      121 ctgaaaaaca tttttttgtc ctctttagcc taagaaagcg taagtttcgt gcagaaattt
      181 gctcctggat cgagcgtagc gagtaaaaaa gttttatgag cgaagcgaat tccaagtttc
      241 ttttgctttt ttttaaagtc acgccagtat taaccaaaaa attgccccaa cgaactgagc
      301 gaaagcgaag ttcaatagag tttgagcgaa gcgaaaacca agggcaattt ttcattccct
      361 gggcttttaa ttattttaaa gtttttaaat acttttaaat ggcttgaaag ccttatgaat
      421 aaagggtttt agctcttata tgtccacgtt taccttgcaa tatgtccacg tttaccttgc
      481 aatatgtcca cgtttacctt gcaatatgtc cacgtttacc ttgcattagt acacaaataa
      541 tattaacgtg tacttataca caataaaaaa tagtggctat gagagattta gttgtaaagg
      601 acaatgcctt aatcaacgca agctataact tagacttagt agaacaacgt ttaattttat
      661 tggctattgt tgaagcaagg gaaagtggga aagggattaa tgcaaatgat ccattagaag
      721 ttcatgcaga tagttatatc aatcaatttg gtgtacaccg taatacagct tatcaagcct
      781 taaaagatgc ttgtaaggat ttattcgcgc gtcaatttag ctatcaagag aaaaaagcta
      841 atgggaatat ccgaaatgtt atgagtcgtt gggtatctca aattgcttat aacgacaatg
      901 aagcaactgt agacttaata tttgcacctg ctgttgttcc cttcataacc cgactggaag
      961 aacaatttac taaatatgaa ttacagcaag ttagtagtct tagtagcgct tatgccattc
     1021 gcttatatga gcttctaatt cagtggcgaa gcactggtaa aaccccaacc atagaactac
     1081 aagaatttag aaagaagtta ggcgttcttg ataatgaata tttacggatg gctcatttaa
     1141 aagagcgtgt tttagagctt tcaattaaac aaataaatga gcatacggat ataactgtaa
     1201 aatatgaaca gcataaaaga ggacgttcta tttcaggatt ttcttttacc tttaaacaga
     1261 agaagaagga tagcccatca atagaaagag atccgaacac tttggagctt ttttcaaaga
     1321 tgaccgatgc tcaacggcat atgtttgcaa ataaactttc agaactccct gaaatgggtc
     1381 gctattcaca aggaacagaa agctaccaac agtttgctgt acgtattgct gagatgctac
     1441 aagatcccgc tcaattcaaa gagctatacc catacctaaa aaaagtggga tacatgccat
     1501 caaataaaaa ggacaccgta aatggctaag ttatcactaa gtgaagtatc taaaaaattt
     1561 catgtggata gatcaaccat ttacagagct gtacgtaatg gacgtttatc acgctccagt
     1621 gatggacaat tcgatctagc agaggtcata cgatgctttg gagaacctga gcaaacatct
     1681 caaaaaattg aatcatctaa gcaagaaggt gatgaatcta caaaaaaact tattgcccat
     1741 ttagaaaatg aagtcaaaaa ataccaagaa cgtgaagaac ggttaatgca acaaattgac
     1801 cgtatgcaaa cactcattga gctgaaaagt gttgcacctg ccacagcagc accacaccaa
     1861 gatgctacgg catgcgacac caagatgcca cagcatgcga caacacaaca agacactgat
     1921 aacaaaaaga ataatgaatt aaatattgca gaaaatgtag cagtgccaca gcaagaaact
     1981 acggcatacc acacccaaac gctacagcat gccacgttgc aaagtgtggc agtgccacaa
     2041 cacaaaaaac gtggtttatt tggccgtgtg ctgaatgccg tctttgataa tgactgagca
     2101 ataagggtga aagatcatgc cgaaactgaa agacattgcc ctgggaatta ttgtagctcc
     2161 gctgctgatc ccgatcatgc tgattgcatc gtaccaggat aaaaaggcgc tgaagaaaga
     2221 gctggatgaa tgccaaaaag aaaaagacca ggcatcctga ttattcaatt gagctaatca
     2281 aaagtgtgga acattgatga aataatagcg tggaacgtga aaagtcataa aaaaagcccg
     2341 aacagggatg ctcgggctat aaactagaaa ctacagcgtt gaattataag gataattata
     2401 cagcatttcg tataaggcgt attatgttaa ttttagaaat tattaaatga ttccaagatt
     2461 ttctagcgac ttataagtaa tttcattacg aatagaacca gacattcctt ctttcatttc
     2521 taagttgagc gaaaagggga ttttttttcc attagcttgc tccacccaac cagtcaacca
     2581 acctacctgt ggagtaacat ccattcccca tccacttttt gcataaatct tactaccatt
     2641 tacttcttta attagaagca tttttttaac ttcttcttga gtttctaatt taaaaggtaa
     2701 tcggttatgt gcaaggtcat cggcaaaatt aacttcttgt actggtgtaa ttttaagggg
     2761 gccaactaac caaaaattat cgacctgtgt tccaatattt gtatttccaa aattaacccg
     2821 ctttacttct ttctgcatta gctctaggcc agtccgtctt gcaagctctt gatatactgg
     2881 aactgctgac aatgccattg cctcacctaa agtcatatct ttctcccaca taggataagt
     2941 tcttttttta ccatcccatt tgaaaatctc atttgttgtt gctttatgat tttctagccc
     3001 gattaaagca tttagcatct taaatgttga tgcagggaca tattctttat ttgctcgtgc
     3061 aagagcatta ccataggtgc taagattttt accctcttta ataataatta caccctgtgt
     3121 ttgagcttca tcaaaatagc ttttaatagc tttttcatgt tgctgagaag aaatatgaaa
     3181 attatcttca gatttagttt taatagatga acatgcactg agagaaacta gaatagaaat
     3241 gctgaatata ggaagtataa attttttcat tacaaattca tgttagggga aattttgggg
     3301 cttagagtat aatattttta ttaaaaaagt taaaactctt ttaatttaac ataatggacg
     3361 ttatgcgaag tacacttgta tattaatata aatatcaact acttaataaa aatagaaaaa
     3421 tcccaaaatg cccaaaaagc cgactttgaa acaagtcggc ttttttatga gcagttttga
     3481 tacttcttgc caataatttt gactaaaaat tacacgaatc tggttgagtg atacaatttt
     3541 gtgatacatt attgtatata caaatttgaa tgttttttat ggaacagtat tttgaatggg
     3601 atgaggctaa gaatcgaaag aatcagaaaa agcatgatat ctctttcgaa actgcaagcc
     3661 ttgtttttga agatccatta cggatctcaa tccaagacag acataccaat ggtgaagaac
     3721 gttggcaaac cattggacga gttaaaggcg tactaatgtt attggtggct cacaccattt
     3781 ttgatgaaga tgactgtgaa atcatccgaa tcattagtgc aaggcaagtc actaaagcgg
     3841 agcgaaacaa atatgagcat ggttagatac tcacacaaag agctgaatga gaaatttggt
     3901 gagaagcaag atgctgaaat tcaacgcttg cttgctaaag gcacggttcc tgatgatcag
     3961 ctagatctat cagatattcc tgaaattaca gactggagca atgctgtacg ccaaaatcaa
     4021 ttttaccgcc cagtaaagca acaaacttca attcggttag atgctgatgt acttgcttgg
     4081 ttcaaagcac aaggcaaagg ttatcaaaca cgtatgaatg aaattttacg agatgctatg
     4141 ctaaaagaat taaaaaatca tcaataagaa tgggagctta ggctcccatt tttgacttca
     4201 tttttagggg ggattaccat tcaaaaattt aaatagtaga ttttataaat ttttaagtgt
     4261 tatattataa tataacatta aatttggttg ttttatgcta aataaaagta aactattttt
     4321 agctttaatt actttagggg cctcgaaaat tttattggca gctgaagggc ctgttactac
     4381 cttaaataca atagtattaa ctgcacaatc tgatgagtta gggtctgagc tgttaggcaa
     4441 aagtctaaat gttagtaatc agtttattga tactagtaaa cttaaacagc gttcgacaac
     4501 tctaggggat gccttgggta cggaattagg aattcattct aatcaatatg gaggtggagc
     4561 atctactcct attattcgtg ggcaggaagg aaaacgtatt aaagttttac agaataatgc
     4621 tgacgtgttg gatatgtcta atatgtcccc agatcacgct gtcacagttg aaccaagttt
     4681 agctaagtct attgaaatta tacgtggtgc aagtaccctt ttgtatagtt caaatagtgc
     4741 ggcaggggta gtcaatgtca ttgactataa aattccaacc caaatgcctc aagacggatt
     4801 agagggtaat actacattac gttttaatac tggcagtaat gagaagttga ccacagcagg
     4861 tgtaacagtt ggcttaagcc cacatgtagc tttacgggca gaagggttat atcgtaatgc
     4921 tggtaactat aaaactcctc attaccagtc tagctcttat aattctttag aagatcttga
     4981 aaaccaaaac agtatttata aaaatttaaa atatttacca gaaagctggg cagagtcgag
     5041 agtaggtacg cttggattat cctggataga cgataatact tatttaggag tttcttatac
     5101 acatcgtcat gatgaatatg gtttaccagc ccatagccat ttatatgaag gttgtggtgc
     5161 ctctgcaatc ggtatcgatt cacgaattag tggattaaaa aattatcttt tatattatcc
     5221 tcaattgatg gatgagcaag atattaatta cataaatcca cgtcctgatt gtcatcagca
     5281 taatcatatt catgaaacta atttctctca taatgctcct tatattgatt taaatactcg
     5341 acgttatgat gtgcgtggag aattcactca accatttacc ggtattgaca aaattcgtac
     5401 ctcattgagt tatatagatt attttcacaa tgaactcgag ggagataaga ttactaactt
     5461 ttttaaaaat acaggaaaag taggccgcat agagttatca catcagccat taggggaatt
     5521 aaccggtatc ttggggctac aatatttgga acaggataat agtgctttat caccagtcca
     5581 ttctcaagaa ggccatacca cctatctaga taatcagcag ctattaaatc gaaatgtcac
     5641 taaaaacttt agtgtatttg gcctagaaaa atataactgg aacgacttta ctttcgaact
     5701 aggtgctcgt atagaaaagc agaaagttag tatggattat gatatagaaa aaattaaaga
     5761 ttcaatgaaa ccttggccta ataaatataa cagtccttat gtagaaaaaa ataataaaat
     5821 cagggctcaa aatttaaaat ctattttaga agctgttcaa cctaacaaag aaacagcttt
     5881 ttcttatgca ggtacagtcc actggagatt tgctccaaat tatatcttat cattaacagg
     5941 tacacatcaa gaaagattac ctaatgcaca agaaatgtat actcatggta tgcatctcgc
     6001 tactaattct tttgaaatag gtaaccgctt tctaagaaaa gagaaatcca ataatttaga
     6061 aatcagtctg gcctataagg atgatttact tgattatcaa atcagtactt actattatga
     6121 ttttgataac tatatatatt tacagacact caatgaagtt ttaggaacta caaaagttag
     6181 agatcagcat acactacgta ttaatcatta tagccagtct gctgctaatt tttatggatt
     6241 agagggcaac atagggtatc aatttaattc tgtttatcac ggctctcttt ttggagatta
     6301 tgtgaaaggc cgtttaacaa atttacctga tgctgtaatt gcatacgata tctggaatag
     6361 agaaccgact ttagctcccc aaaaggatcg ttacactcct agattacctc ctgctcgttt
     6421 gggtacacga ctaaaagctg attttgatga atcgcttaaa ggtgaaatag aatattatcg
     6481 agtctttaaa caagataata tttctaaatt tgagcaggtt acatcaggtt ataatatgct
     6541 taatatgact ttagcatata aaaataagct atctcacaca gaatatgatt tattctttaa
     6601 agcgaataat ttacttgatc agaaagtcta tgcccatgaa acatttttgc catatattcc
     6661 ccaaattgga agaaatttta gtttaggctt aaatcttaat ttctagataa gatactgtaa
     6721 tcttctaaga aaatttctta gaagattttt cttataaaat gattagatta ataattaagg
     6781 taagcgtccg ctgaatccca tacctatttt ttaattcgat ttaggtttcc agcagtgtgg
     6841 cagtaactct tcaatctggg tcactttatg tgtcggcagc cttttcagca ctgaatagcc
     6901 accgattttg tagacacctt ttagctagat aaaaagcaat ttttttgatc tttaaagaat
     6961 gaggatataa gctgtttaaa acttaattct aattggttaa taggtgaaaa atgaaattag
     7021 cagaagctct cttacttcga agtgatcaac aaaaaaaaat tatttcacta aaacaacgta
     7081 tcaatgcaaa tgtattggta caagatggtg atcaaccatc tgaagatcca aatgaactac
     7141 taaaacaagt attttcttta attcaagaat ttcaaaaatt aagttatgca attcatgaaa
     7201 ctaatgcttt aacaaaatta aatgatggcc gttctctact tgctttattg actttgcgtg
     7261 atgaatttgt agaacagcac aaaaccctaa ctgcagcaat aagtaataca tctcgtgaat
     7321 ctgatcgtta tagtacgcgg gaaataaagt ggcataaagt tattccagtt tcatcattac
     7381 aaaaacaagc tgatgatatt agtcttaaat tacgtgattt aaacgtgtta attcagtcga
     7441 ctaattggaa aattgatttg attgaagcct aatagtcctt tagatataat caagttgaac
     7501 atactgggcg agtacaagat cccttacttc cacaacatag gattgtaaat aaagtgtagt
     7561 ggcttgcaaa gcaagtggat aagcttaaaa ttttaagtac aaatcaatcc atcacttggc
     7621 ataactcaaa tctttgtgca aacaaagtta cacgttactt ataactacca tgtactgaca
     7681 gtatgttcac ctcaattact aaaaataaag aaatgaacaa gcatcagctc aactacattt
     7741 ctatttttaa ttcagttcgg catttaacat aaaatctctt atacgaagcc caggttaatt
     7801 ctgggctttt tgttcaatct catcattggc cgagaacata cttggattcg taagcctgat
     7861 attttcctga caacttccac acagcatcaa tcctgcaaaa agttgtacat tttgtctatt
     7921 ttcacaaagc gtacatggct ttgaaagctg aataatatta tctgacacta ctttaccctc
     7981 ttatagttat catctgcata tgaataacct ttagcttgta tcattctaac ctcatcttta
     8041 gaagcgccat ttttcattaa gagccacaac atcatgaaat atagtccaga aaagtcttca
     8101 tagtattttt cagaaaaatt gttatcttcc tcaaaatcat ctattcgctt ttctaatcga
     8161 ttcaccagat cctcaatacg atcggcttgc tgtttaacag caatagattg aaaatcattg
     8221 gtcaaaattt tactgaataa tttactggtt ctgattcctt ctgaattagc caattgatcg
     8281 tagaattctg ctacttgtgg atctaacctg actgcataag ttttgacttt ttcgcccata
     8341 aattacacct cttattaaaa ttagattttg tacaaaatta aatatatata attcaactaa
     8401 ttaaataaaa aataataatt agattacgta caaacttatt tgtacagaat taatttattc
     8461 taaaacaaaa acttatttaa atataatgct ttttttatgt acaatcatga gtacatattt
     8521 tttcttaaat ttatcaatat cttatctaaa aaaagtgttg ttttgatgta caaccatgag
     8581 cacagcataa aaaatagata attcaatcac ttattctaaa aaaagaccat ttttctgtac
     8641 acgtttctga gaccctaaaa aatcgctgaa atccttatcc tgtaaggctt tgaggggtgt
     8701 catcgttaga tgcgtggtgt gtgacaaaaa aatcccggca tgtgccggat cttggattgg
     8761 aaaattggct aaagtgacgt agggccggtg cttggttttg catggaaaaa agtatttatt
     8821 tccttgttta gaaaaacgta aaaaatcaat tttttgtcca ttcatccagg ttaaaaattt
     8881 caacccaaaa ctttaattat gaaaagcttc atagaacgcg ttcaaatgcg atttaagagc
     8941 ctttacctag aaaa
//
