LOCUS       NZ_CP076737             8970 bp    DNA     circular CON 20-APR-2024
DEFINITION  Acinetobacter baumannii strain X4-201 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP076737
VERSION     NZ_CP076737.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19690115
            Assembly: GCF_029774315.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Hsieh,Y.C., Wu,J.W., Chen,Y.Y., Quyen,T.L.T., Liao,W.C., Li,S.W.,
            Chen,Y.C. and Pan,Y.J.
  TITLE     An Outbreak of tet(X6)-Carrying Tigecycline-Resistant Acinetobacter
            baumannii Isolates with a New Capsular Type at a Hospital in Taiwan
  JOURNAL   Antibiotics (Basel) 10 (10), 1239 (2021)
   PUBMED   34680819
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8970)
  AUTHORS   Pan,Y.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) Department of Microbiology and Immunology,
            China Medical University, Taichung, Taiwan, No.91 Hsueh-Shih Road,
            Taichung 40402, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 210x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029774315.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 06:57:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,865
            Genes (coding)                    :: 3,768
            CDSs (with protein)               :: 3,768
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 40 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 19 of 97
            Pseudo Genes (multiple problems)  :: 11 of 97
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076737.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="X4-201"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed1"
                     /geo_loc_name="Taiwan"
                     /collection_date="2020-03-03"
     gene            complement(74..328)
                     /locus_tag="KRO90_RS19880"
     CDS             complement(74..328)
                     /locus_tag="KRO90_RS19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            1012..1962
                     /gene="repM"
                     /locus_tag="KRO90_RS19200"
                     /old_locus_tag="KRO90_19070"
     CDS             1012..1962
                     /gene="repM"
                     /locus_tag="KRO90_RS19200"
                     /old_locus_tag="KRO90_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            1955..2530
                     /locus_tag="KRO90_RS19205"
                     /old_locus_tag="KRO90_19075"
     CDS             1955..2530
                     /locus_tag="KRO90_RS19205"
                     /old_locus_tag="KRO90_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_279482303.1"
                     /translation="MAKLSLSEVFKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            2550..2693
                     /locus_tag="KRO90_RS19210"
                     /old_locus_tag="KRO90_19080"
     CDS             2550..2693
                     /locus_tag="KRO90_RS19210"
                     /old_locus_tag="KRO90_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(2876..3703)
                     /gene="blaOXA"
                     /locus_tag="KRO90_RS19215"
                     /old_locus_tag="KRO90_19085"
     ARG             complement(2880..3703)
                     /name="blaOXA"
                     /gene="blaOXA"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.91"
                     /coverage="100.0"
     gene            4012..4299
                     /locus_tag="KRO90_RS19220"
                     /old_locus_tag="KRO90_19090"
     CDS             4012..4299
                     /locus_tag="KRO90_RS19220"
                     /old_locus_tag="KRO90_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            4286..4600
                     /locus_tag="KRO90_RS19225"
                     /old_locus_tag="KRO90_19095"
     CDS             4286..4600
                     /locus_tag="KRO90_RS19225"
                     /old_locus_tag="KRO90_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            4728..7139
                     /gene="znuD2"
                     /locus_tag="KRO90_RS19230"
                     /old_locus_tag="KRO90_19100"
     CDS             4728..7139
                     /gene="znuD2"
                     /locus_tag="KRO90_RS19230"
                     /old_locus_tag="KRO90_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            7444..7905
                     /locus_tag="KRO90_RS19235"
                     /old_locus_tag="KRO90_19105"
     CDS             7444..7905
                     /locus_tag="KRO90_RS19235"
                     /old_locus_tag="KRO90_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(8228..8401)
                     /locus_tag="KRO90_RS19240"
                     /old_locus_tag="KRO90_19110"
     CDS             complement(8228..8401)
                     /locus_tag="KRO90_RS19240"
                     /old_locus_tag="KRO90_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(8401..8772)
                     /locus_tag="KRO90_RS19245"
                     /old_locus_tag="KRO90_19115"
     CDS             complement(8401..8772)
                     /locus_tag="KRO90_RS19245"
                     /old_locus_tag="KRO90_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     ARG             complement(2877..3703)
                     /name="OXA-72"
                     /gene="OXA-72"
                     /gene_name="OXA beta-lactamase; OXA-24-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; penam"
                     /identity="98.91"
                     /coverage="100.0"
     Typing          1013..1962
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tatcttatct aaaaaaagtg ttgttttgat gtacaaccat gagcacagca taaaaaatag
       61 ataattcaat cacttattct aaaaaaagac catttttctg tacacgtttc tgagacccta
      121 aaaaatcgct gaaatcctta tcctgtaagg ctttgagggg tgtcatcgtt agatgcgtgg
      181 tgtgtgacaa aaaaatcccg gcatgtgccg gatcttggat tggaaaattg gctaaagtga
      241 cgtagggccg gtgcttggtt ttgcatggaa aaaagtattt atttccttgt ttagaaaaac
      301 gtaaaaaatc aattttttgt ccattcatcc aggttaaaaa tttcaaccca aaactttaat
      361 tatgaaaagc ttcatagaac gcgttcaaat gcgatttaag agcctttacc tagaaaacat
      421 agatctcata gtgaaaaaca caaaacagct caaaaaacgc aaatgagagc gaaataaaga
      481 ggtattttga ctttagatag atgcatagag cgagtgtcta cgagcgaact atcaaaattt
      541 gcgcctagac cctctgaaaa acattttttt gtcctcttta gcctaagaaa gcgtaagttt
      601 cgtgcagaaa tttgctcctg gatcgagcgt agcgagtaaa aaagttttat gagcgaagcg
      661 aattccaagt ttcttttgct tttttttaaa gtcacgccag tattaaccaa aaaattgccc
      721 caacgaactg agcgaaagcg aagttcaata gagtttgagc gaagcgaaaa ccaagggcaa
      781 tttttcattc cctgggcttt taattatttt aaagttttta aatactttta aatggcttga
      841 aagccttatg aataaagggt tttagctctt atatgtccac gtttaccttg caatatgtcc
      901 acgtttacct tgcaatatgt ccacgtttac cttgcaatat gtccacgttt accttgcatt
      961 agtacacaaa taatattaac gtgtacttat acacaataaa aaatagtggc tatgagagat
     1021 ttagttgtaa aggacaatgc cttaatcaac gcaagctata acttagactt agtagaacaa
     1081 cgtttaattt tattggctat tgttgaagca agggaaagtg ggaaagggat taatgcaaat
     1141 gatccattag aagttcatgc agatagttat atcaatcaat ttggtgtaca ccgtaataca
     1201 gcttatcaag ccttaaaaga tgcttgtaag gatttattcg cgcgtcaatt tagctatcaa
     1261 gagaaaaaag ctaatgggaa tatccgaaat gttatgagtc gttgggtatc tcaaattgct
     1321 tataacgaca atgaagcaac tgtagactta atatttgcac ctgctgttgt tcccttcata
     1381 acccgactgg aagaacaatt tactaaatat gaattacagc aagttagtag tcttagtagc
     1441 gcttatgcca ttcgcttata tgagcttcta attcagtggc gaagcactgg taaaacccca
     1501 accatagaac tacaagaatt tagaaagaag ttaggcgttc ttgataatga atatttacgg
     1561 atggctcatt taaaagagcg tgttttagag ctttcaatta aacaaataaa tgagcatacg
     1621 gatataactg taaaatatga acagcataaa agaggacgtt ctatttcagg attttctttt
     1681 acctttaaac agaagaagaa ggatagccca tcaatagaaa gagatccgaa cactttggag
     1741 cttttttcaa agatgaccga tgctcaacgg catatgtttg caaataaact ttcagaactc
     1801 cctgaaatgg gtcgctattc acaaggaaca gaaagctacc aacagtttgc tgtacgtatt
     1861 gctgagatgc tacaagatcc cgctcaattc aaagagctat acccatacct aaaaaaagtg
     1921 ggatacatgc catcaaataa aaaggacacc gtaaatggct aagttatcac taagtgaagt
     1981 atttaaaaaa tttcatgtgg atagatcaac catttacaga gctgtacgta atggacgttt
     2041 atcacgctcc agtgatggac aattcgatct agcagaggtc atacgatgct ttggagaacc
     2101 tgagcaaaca tctcaaaaaa ttgaatcatc taagcaagaa ggtgatgaat ctacaaaaaa
     2161 acttattgcc catttagaaa atgaagtcaa aaaataccaa gaacgtgaag aacggttaat
     2221 gcaacaaatt gaccgtatgc aaacactcat tgagctgaaa agtgttgcac ctgccacagc
     2281 agcaccacac caagatgcta cggcatgcga caccaagatg ccacagcatg cgacaacaca
     2341 acaagacact gataacaaaa agaataatga attaaatatt gcagaaaatg tagcagtgcc
     2401 acagcaagaa actacggcat accacaccca aacgctacag catgccacgt tgcaaagtgt
     2461 ggcagtgcca caacacaaaa aacgtggttt atttggccgt gtgctgaatg ccgtctttga
     2521 taatgactga gcaataaggg tgaaagatca tgccgaaact gaaagacatt gccctgggaa
     2581 ttattgtagc tccgctgctg atcccgatca tgctgattgc atcgtaccag gataaaaagg
     2641 cgctgaagaa agagctggat gaatgccaaa aagaaaaaga ccaggcatcc tgattattca
     2701 attgagctaa tcaaaagtgt ggaacattga tgaaataata gcgtggaacg tgaaaagtca
     2761 taaaaaaagc ccgaacaggg atgctcgggc tataaactag aaactacagc gttgaattat
     2821 aaggataatt atacagcatt tcgtataagg cgtattatgt taattttaga aattattaaa
     2881 tgattccaag attttctagc gacttataag taatttcatt acgaatagaa ccagacattc
     2941 cttctttcat ttctaagttg agcgaaaaag gggatttttt tccattagct tgctccaccc
     3001 aaccagtcaa ccaacctacc tgtggagtaa catccattcc ccatccactt tttgcataaa
     3061 tcttactacc atttacttct ttaattagaa gcattttttt aacttcttct tgagtttcta
     3121 atttaaaagg taatcggtta tgtgcaaggt catcggcaaa attaacttct tgtactggtg
     3181 taattttaag ggggccaact aaccaaaaat tatcgacctg tgttccaata tttgtatttc
     3241 caaaattaac ccgctttact tctttctgca ttagctctag gccagtccgt cttgcaagct
     3301 cttgatatac tggaactgct gacaatgcca ttgcctcacc taaagtcata tctttctccc
     3361 acataggata agttcttttt ttaccatccc atttgaaaat ctcatttgtt gttgctttat
     3421 gattttctag cccgattaaa gcatttagca tcttaaatgt tgatgcaggg acatattctt
     3481 tatttgctcg tgcaagagca ttaccatagg tgctaagttt tttaccctct ttaataataa
     3541 ttacaccctg tgtttgagct tcatcaaaat agcttttaat agctttttca tgttgctgag
     3601 aagaaatatg aaaattatct tcagatttag ttttaataga tgaacatgca ctgagagaaa
     3661 ctagaataga aatgctgaat ataggaagta ttaatttttt cattacaaat tcatgttagg
     3721 ggaaattttg gggcttagag tataatattt ttattaaaaa agttaaaact cttttaattt
     3781 aacataatgg acgttatgcg aagtacactt gtatattaat ataaatatca actacttaat
     3841 aaaaatagaa aaatcccaaa atgcccaaaa agccgacttt gaaacaagtc ggctttttta
     3901 tgagcagttt tgatacttct tgccaataat tttgactaaa aattacacga atctggttga
     3961 gtgatacaat tttgtgatac attattgtat atacaaattt gaatgttttt tatggaacag
     4021 tattttgaat gggatgaggc taagaatcga aagaatcaga aaaagcatga tatctctttc
     4081 gaaactgcaa gccttgtttt tgaagatcca ttacggatct caatccaaga cagacatacc
     4141 aatggtgaag aacgttggca aaccattgga cgagttaaag gcgtactaat gttattggtg
     4201 gctcacacca tttttgatga agatgactgt gaaatcatcc gaatcattag tgcaaggcaa
     4261 gtcactaaag cggagcgaaa caaatatgag catggttaga tactcacaca aagagctgaa
     4321 tgagaaattt ggtgagaagc aagatgctga aattcaacgc ttgcttgcta aaggcacggt
     4381 tcctgatgat cagctagatc tatcagatat tcctgaaatt acagactgga gcaatgctgt
     4441 acgccaaaat caattttacc gcccagtaaa gcaacaaact tcaattcggt tagatgctga
     4501 tgtacttgct tggttcaaag cacaaggcaa aggttatcaa acacgtatga atgaaatttt
     4561 acgagatgct atgctaaaag aattaaaaaa tcatcaataa gaatgggagc ttaggctccc
     4621 atttttgact tcatttttag gggggattac cattcaaaaa tttaaatagt agattttata
     4681 aatttttaag tgttatatta taatataaca ttaaatttgg ttgttttatg ctaaataaaa
     4741 gtaaactatt tttagcttta attactttag gggcctcgaa aattttattg gcagctgaag
     4801 ggcctgttac taccttaaat acaatagtat taactgcaca atctgatgag ttagggtctg
     4861 agctgttagg caaaagtcta aatgttagta atcagtttat tgatactagt aaacttaaac
     4921 agcgttcgac aactctaggg gatgccttgg gtacggaatt aggaattcat tctaatcaat
     4981 atggaggtgg agcatctact cctattattc gtgggcagga aggaaaacgt attaaagttt
     5041 tacagaataa tgctgacgtg ttggatatgt ctaatatgtc cccagatcac gctgtcacag
     5101 ttgaaccaag tttagctaag tctattgaaa ttatacgtgg tgcaagtacc cttttgtata
     5161 gttcaaatag tgcggcaggg gtagtcaatg tcattgacta taaaattcca acccaaatgc
     5221 ctcaagacgg attagagggt aatactacat tacgttttaa tactggcagt aatgagaagt
     5281 tgaccacagc aggtgtaaca gttggcttaa gcccacatgt agctttacgg gcagaagggt
     5341 tatatcgtaa tgctggtaac tataaaactc ctcattacca gtctagctct tataattctt
     5401 tagaagatct tgaaaaccaa aacagtattt ataaaaattt aaaatattta ccagaaagct
     5461 gggcagagtc gagagtaggt acgcttggat tatcctggat agacgataat acttatttag
     5521 gagtttctta tacacatcgt catgatgaat atggtttacc agcccatagc catttatatg
     5581 aaggttgtgg tgcctctgca atcggtatcg attcacgaat tagtggatta aaaaattatc
     5641 ttttatatta tcctcaattg atggatgagc aagatattaa ttacataaat ccacgtcctg
     5701 attgtcatca gcataatcat attcatgaaa ctaatttctc tcataatgct ccttatattg
     5761 atttaaatac tcgacgttat gatgtgcgtg gagaattcac tcaaccattt accggtattg
     5821 acaaaattcg tacctcattg agttatatag attattttca caatgaactc gagggagata
     5881 agattactaa cttttttaaa aatacaggaa aagtaggccg catagagtta tcacatcagc
     5941 cattagggga attaaccggt atcttggggc tacaatattt ggaacaggat aatagtgctt
     6001 tatcaccagt ccattctcaa gaaggccata ccacctatct agataatcag cagctattaa
     6061 atcgaaatgt cactaaaaac tttagtgtat ttggcctaga aaaatataac tggaacgact
     6121 ttactttcga actaggtgct cgtatagaaa agcagaaagt tagtatggat tatgatatag
     6181 aaaaaattaa agattcaatg aaaccttggc ctaataaata taacagtcct tatgtagaaa
     6241 aaaataataa aatcagggct caaaatttaa aatctatttt agaagctgtt caacctaaca
     6301 aagaaacagc tttttcttat gcaggtacag tccactggag atttgctcca aattatatct
     6361 tatcattaac aggtacacat caagaaagat tacctaatgc acaagaaatg tatactcatg
     6421 gtatgcatct cgctactaat tcttttgaaa taggtaaccg ctttctaaga aaagagaaat
     6481 ccaataattt agaaatcagt ctggcctata aggatgattt acttgattat caaatcagta
     6541 cttactatta tgattttgat aactatatat atttacagac actcaatgaa gttttaggaa
     6601 ctacaaaagt tagagatcag catacactac gtattaatca ttatagccag tctgctgcta
     6661 atttttatgg attagagggc aacatagggt atcaatttaa ttctgtttat cacggctctc
     6721 tttttggaga ttatgtgaaa ggccgtttaa caaatttacc tgatgctgta attgcatacg
     6781 atatctggaa tagagaaccg actttagctc cccaaaagga tcgttacact cctagattac
     6841 ctcctgctcg tttgggtaca cgactaaaag ctgattttga tgaatcgctt aaaggtgaaa
     6901 tagaatatta tcgagtcttt aaacaagata atatttctaa atttgagcag gttacatcag
     6961 gttataatat gcttaatatg actttagcat ataaaaataa gctatctcac acagaatatg
     7021 atttattctt taaagcgaat aatttacttg atcagaaagt ctatgcccat gaaacatttt
     7081 tgccatatat tccccaaatt ggaagaaatt ttagtttagg cttaaatctt aatttctaga
     7141 taagatactg taatcttcta agaaaatttc ttagaagatt tttcttataa aatgattaga
     7201 ttaataatta aggtaagcgt ccgctgaatc ccatacctat tttttaattc gatttaggtt
     7261 tccagcagtg tggcagtaac tcttcaatct gggtcacttt atgtgtcggc agccttttca
     7321 gcactgaata gccaccgatt ttgtagacac cttttagcta gataaaaagc aatttttttg
     7381 atctttaaag aatgaggata taagctgttt aaaacttaat tctaattggt taataggtga
     7441 aaaatgaaat tagcagaagc tctcttactt cgaagtgatc aacaaaaaaa aattatttca
     7501 ctaaaacaac gtatcaatgc aaatgtattg gtacaagatg gtgatcaacc atctgaagat
     7561 ccaaatgaac tactaaaaca agtattttct ttaattcaag aatttcaaaa attaagttat
     7621 gcaattcatg aaactaatgc tttaacaaaa ttaaatgatg gccgttctct acttgcttta
     7681 ttgactttgc gtgatgaatt tgtagaacag cacaaaaccc taactgcagc aataagtaat
     7741 acatctcgtg aatctgatcg ttatagtacg cgggaaataa agtggcataa agttattcca
     7801 gtttcatcat tacaaaaaca agctgatgat attagtctta aattacgtga tttaaacgtg
     7861 ttaattcagt cgactaattg gaaaattgat ttgattgaag cctaatagtc ctttagatat
     7921 aatcaagttg aacatactgg gcgagtacaa gatcccttac ttccacaaca taggattgta
     7981 aataaagtgt agtggcttgc aaagcaagtg gataagctta aaattttaag tacaaatcaa
     8041 tccatcactt ggcataactc aaatctttgt gcaaacaaag ttacacgtta cttataacta
     8101 ccatgtactg acagtatgtt cacctcaatt actaaaaata aagaaatgaa caagcatcag
     8161 ctcaactaca tttctatttt taattcagtt cggcatttaa cataaaatct cttatacgaa
     8221 gcccaggtta attctgggct ttttgttcaa tctcatcatt ggccgagaac atacttggat
     8281 tcgtaagcct gatattttcc tgacaacttc cacacagcat caatcctgca aaaagttgta
     8341 cattttgtct attttcacaa agcgtacatg gctttgaaag ctgaataata ttatctgaca
     8401 ctactttacc ctcttatagt tatcatctgc atatgaataa cctttagctt gtatcattct
     8461 aacctcatct ttagaagcgc catttttcat taagagccac aacatcatga aatatagtcc
     8521 agaaaagtct tcatagtatt tttcagaaaa attgttatct tcctcaaaat catctattcg
     8581 cttttctaat cgattcacca gatcctcaat acgatcggct tgctgtttaa cagcaataga
     8641 ttgaaaatca ttggtcaaaa ttttactgaa taatttactg gttctgattc cttctgaatt
     8701 agccaattga tcgtagaatt ctgctacttg tggatctaac ctgactgcat aagttttgac
     8761 tttttcgccc ataaattaca cctcttatta aaattagatt ttgtacaaaa ttaaatatat
     8821 ataattcaac taattaaata aaaaataata attagattac gtacaaactt atttgtacag
     8881 aattaattta ttctaaaaca aaaacttatt taaatataat gcttttttta tgtacaatca
     8941 tgagtacata ttttttctta aatttatcaa
//
