LOCUS       NZ_CP076692            25878 bp    DNA     linear   CON 18-JUL-2023
DEFINITION  Escherichia coli strain S21EC plasmid pS21EC_B.
ACCESSION   NZ_CP076692
VERSION     NZ_CP076692.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19700015
            Assembly: GCF_018972225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25878)
  AUTHORS   Alvarez-Fraga,L., Phan,M.D., Goh,K.G.K., Nguyen,T.K.N.,
            Hancock,S.J., Allsopp,L.P., Peters,K.M., Forde,B.M., Roberts,L.W.,
            Totsika,M., Beatson,S.A., Ulett,G.C. and Schembri,M.A.
  TITLE     Differential Afa/Dr fimbriae expression in the multidrug resistant
            Escherichia coli ST131 clone
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 25878)
  AUTHORS   Alvarez-Fraga,L., Phan,M.D., Goh,K.G.K., Nguyen,T.K.N.,
            Hancock,S.J., Allsopp,L.P., Peters,K.M., Forde,B.M., Roberts,L.W.,
            Totsika,M., Beatson,S.A., Ulett,G.C. and Schembri,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) School of Chemistry and Molecular
            Biosciences, University of Queensland, Building 76 Cooper road,
            Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2021
            Assembly Method                   :: flye v. 2.5
            Assembly Name                     :: S21EC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 222x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/18/2023 04:17:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,149
            CDSs (total)                      :: 5,034
            Genes (coding)                    :: 4,863
            CDSs (with protein)               :: 4,863
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 72 of 171
            Pseudo Genes (incomplete)         :: 117 of 171
            Pseudo Genes (internal stop)      :: 34 of 171
            Pseudo Genes (multiple problems)  :: 44 of 171
            Pseudo Genes (short protein)      :: 3 of 171
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076692.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..25878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S21EC"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pS21EC_B"
                     /geo_loc_name="United Kingdom:Manchester"
                     /collection_date="2009"
     gene            complement(<1..67)
                     /locus_tag="KQ260_RS25830"
                     /pseudo=""
     CDS             complement(<1..67)
                     /locus_tag="KQ260_RS25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(64..>204)
                     /locus_tag="KQ260_RS25430"
                     /old_locus_tag="KQ260_25385"
                     /pseudo=""
     CDS             complement(64..>204)
                     /locus_tag="KQ260_RS25430"
                     /old_locus_tag="KQ260_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            308..1480
                     /gene="istA"
                     /locus_tag="KQ260_RS25435"
                     /old_locus_tag="KQ260_25390"
     CDS             308..1480
                     /gene="istA"
                     /locus_tag="KQ260_RS25435"
                     /old_locus_tag="KQ260_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="WP_000952372.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPPA
                     RKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKMRP
                     SKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRRFHVFAAPKQDAEHT
                     YESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRACRPRR
                     ARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKETPEQRF
                     ALEQEHLQPLPDTDFDTSYFDIRHVSRDSYIEVGGNRYSVPEALCGQPVSIRISLDDEL
                     RIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
                     /name="istA"
     gene            1480..2277
                     /gene="istB"
                     /locus_tag="KQ260_RS25440"
                     /old_locus_tag="KQ260_25395"
     CDS             1480..2277
                     /gene="istB"
                     /locus_tag="KQ260_RS25440"
                     /old_locus_tag="KQ260_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase IstB"
                     /protein_id="WP_000544830.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQE
                     WNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVILLG
                     PPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYARVLI
                     LDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAILDRLL
                     HHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVKSGQHQ"
                     /name="istB"
     gene            complement(2271..3101)
                     /locus_tag="KQ260_RS25445"
                     /old_locus_tag="KQ260_25400"
     CDS             complement(2271..3101)
                     /locus_tag="KQ260_RS25445"
                     /old_locus_tag="KQ260_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="WP_001298676.1"
                     /translation="MYFSGEPAKIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLLQ
                     TTEDVRFEPCPGLTAAGRGVLSPENITFTRWLKHLQDGVLLDEQNCLMLHELWLQSGTG
                     QRRWEGLPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLSEKTMPFDLLTV
                     LPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDFDTPW
                     CQPESDVVAELSRRFGCTLEHWYAEQGCNFCGCVNATMFDRYLPFSCPLRYFRY"
                     /name="DUF1281 domain-containing protein"
     gene            complement(3520..4740)
                     /locus_tag="KQ260_RS25450"
                     /old_locus_tag="KQ260_25405"
     CDS             complement(3520..4740)
                     /locus_tag="KQ260_RS25450"
                     /old_locus_tag="KQ260_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc53 family transposase"
                     /protein_id="WP_000343766.1"
                     /translation="MDEKSLYAHILNLTAPWQVKSLTLDENAGSVTVTVGIAENTQLTC
                     PTCRKSCSVHDHRHRKWRHLDTCQFMTLVEADVPRVMCPEHGCQTLPVPWAGSGSRYTL
                     LFESFVLSWLKISTVDAVRKQLKLSWNAVDGIMTRAVKRGLSRIKKPLSVRHMNVDEVA
                     FKKGHRYITVVSDRDGRALALTDDRGTESLASYLRSLTDSQLLAIKTLSMDMNAGYIRA
                     ARIHLPNAVEKIAFDRFHVAKQLGEVVDKTRQNEHPHLPVESRRQAKGTRFLWQYSDKW
                     MTESRQEKLMWLREQMQQTSQCWTLKELAKNIWDRPWSTERRNDWLQWISRASECDVPM
                     MKNAAKTIKKRLYGILNAMRHRVSNGNAEALNSKIRLLRIKARGYRNRERFKLGVMFHY
                     GKLNMAY"
                     /name="ISL3-like element ISEc53 family transposase"
     gene            complement(4759..5277)
                     /locus_tag="KQ260_RS25455"
                     /old_locus_tag="KQ260_25410"
     CDS             complement(4759..5277)
                     /locus_tag="KQ260_RS25455"
                     /old_locus_tag="KQ260_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="WP_000115885.1"
                     /translation="MSVPQTKAELLLAIDKNFSKLISYLNTIPPEITSDKSMDGHAKGT
                     EMSVRDLVSYLLGWNALVVKWIASDAKGLPVDFPETGYKWNQLGLLAQKFYSDYSELSY
                     ELLVAELQTVKNEIVNLINDRTDDILYGRPWYTKWTMGRMISFNTSSPYANANGRLRKW
                     AKNNNISLK"
                     /name="ClbS/DfsB family four-helix bundle protein"
     gene            5412..5660
                     /locus_tag="KQ260_RS25460"
                     /old_locus_tag="KQ260_25415"
     CDS             5412..5660
                     /locus_tag="KQ260_RS25460"
                     /old_locus_tag="KQ260_25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="WP_000619112.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRIDQ
                     GSAPSVSVTGARNDKGKERIQSLLEEIWQDDSWLPAA"
                     /name="DinI-like family protein"
     gene            5657..6094
                     /gene="umuD"
                     /locus_tag="KQ260_RS25465"
                     /old_locus_tag="KQ260_25420"
     CDS             5657..6094
                     /gene="umuD"
                     /locus_tag="KQ260_RS25465"
                     /old_locus_tag="KQ260_25420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="WP_000109079.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDLN
                     SYCISRPAATFFLRTSGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKRLL
                     LRPRPALEPVSDSPEFRTLYPENICIFGIVTHVIHRTRELR"
                     /name="umuD"
     gene            6094..7364
                     /gene="umuC"
                     /locus_tag="KQ260_RS25470"
                     /old_locus_tag="KQ260_25425"
                     /pseudo=""
     CDS             6094..7364
                     /gene="umuC"
                     /locus_tag="KQ260_RS25470"
                     /old_locus_tag="KQ260_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766293.1"
                     /GO_function="GO:0003684 - damaged DNA binding [Evidence
                     IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /name="umuC"
     gene            complement(7369..7761)
                     /locus_tag="KQ260_RS25475"
                     /old_locus_tag="KQ260_25430"
     CDS             complement(7369..7761)
                     /locus_tag="KQ260_RS25475"
                     /old_locus_tag="KQ260_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="WP_000340835.1"
                     /translation="MDDERKRKKYTLYLHPEKAADFQTLEAIESVPRSERGELFRNAFI
                     SGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRTVLTELGASQSV
                     EKMPPSATDSVQEAMNDVRLKMKKLF"
                     /name="plasmid partitioning/stability family protein"
     gene            complement(7766..8737)
                     /gene="parM"
                     /locus_tag="KQ260_RS25480"
                     /old_locus_tag="KQ260_25435"
     CDS             complement(7766..8737)
                     /gene="parM"
                     /locus_tag="KQ260_RS25480"
                     /old_locus_tag="KQ260_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="WP_001103694.1"
                     /translation="MNVYCDDGSTTIKLAWNDNGKICKSLSQNSFRHGWKVDGLGIRQT
                     FNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLAPQPVSLTVTLPI
                     SEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVVDKVG
                     QFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNSGIGVSSVTKAAMSALRMASSDTSFL
                     VADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVNRVYVV
                     GGGAPLIYDSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
                     /name="parM"
     gene            8966..9610
                     /locus_tag="KQ260_RS25485"
                     /old_locus_tag="KQ260_25440"
     CDS             8966..9610
                     /locus_tag="KQ260_RS25485"
                     /old_locus_tag="KQ260_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000633911.1"
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAAK
                     HHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAHQI
                     IAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLEYLE
                     EFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAAKAEIEHLMNEVFGPW"
                     /name="AAA family ATPase"
     gene            9604..9879
                     /locus_tag="KQ260_RS25490"
                     /old_locus_tag="KQ260_25445"
     CDS             9604..9879
                     /locus_tag="KQ260_RS25490"
                     /old_locus_tag="KQ260_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000239529.1"
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQCR
                     TTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
                     /name="hypothetical protein"
     gene            complement(10017..10826)
                     /locus_tag="KQ260_RS25495"
                     /old_locus_tag="KQ260_25450"
     CDS             complement(10017..10826)
                     /locus_tag="KQ260_RS25495"
                     /old_locus_tag="KQ260_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237314.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_000016979.1"
                     /translation="MSGSVIHSQSAARVPAVYSAGQSPQLPVVIDYPAALALRQMSMVH
                     DELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQ
                     LATLKQRTEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPLERRNKRTGR
                     TEKARIWEVTDRTVRTWIGEAVAAAATDGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVL
                     QSLMGHKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNRHA"
                     /name="site-specific integrase"
     gene            complement(10827..11132)
                     /gene="ccdB"
                     /locus_tag="KQ260_RS25500"
                     /old_locus_tag="KQ260_25455"
     CDS             complement(10827..11132)
                     /gene="ccdB"
                     /locus_tag="KQ260_RS25500"
                     /old_locus_tag="KQ260_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="WP_001159871.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
                     /name="ccdB"
     gene            complement(11134..11352)
                     /gene="ccdA"
                     /locus_tag="KQ260_RS25505"
                     /old_locus_tag="KQ260_25460"
     CDS             complement(11134..11352)
                     /gene="ccdA"
                     /locus_tag="KQ260_RS25505"
                     /old_locus_tag="KQ260_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="WP_000813634.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWK
                     AENQEGMAEVARFIEMNGSFADENRDW"
                     /name="ccdA"
     gene            13674..14315
                     /locus_tag="KQ260_RS25510"
                     /old_locus_tag="KQ260_25465"
     CDS             13674..14315
                     /locus_tag="KQ260_RS25510"
                     /old_locus_tag="KQ260_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000990667.1"
                     /translation="MNADTWTRLQAFRHALELTSCEASLTAGYDHLKDFPAGCSELASQ
                     TLTDYLTEDGSNLYSCIVGMQWDNGPGRYGHVIADPARDYIDLTLDQFPGYHNRIVAEP
                     VESGGQLAADLNREPAISTADGIVASPSDKVNYITEQKNRQPMVIITECYKLIRMTARF
                     IPMCQSSQLFQHQVFPRLFIISDVGSCSLFRNTLDRLATDWKVTLSILSL"
                     /name="hypothetical protein"
     gene            15490..16467
                     /locus_tag="KQ260_RS25515"
                     /old_locus_tag="KQ260_25470"
     CDS             15490..16467
                     /locus_tag="KQ260_RS25515"
                     /old_locus_tag="KQ260_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_001309253.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNTL
                     SRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVELP
                     FVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSAYYD
                     TERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPAPISL
                     ARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPRLKAPN
                     DESKENQLPPSPAEKISPEMAEKLALLEKLGITLDDLEKLFKSR"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(16710..17450)
                     /locus_tag="KQ260_RS25520"
                     /old_locus_tag="KQ260_25475"
     CDS             complement(16710..17450)
                     /locus_tag="KQ260_RS25520"
                     /old_locus_tag="KQ260_25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_001066947.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAYL
                     LAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEKVR
                     ARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWPVTDETMRNWLKQAVKRA
                     EADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFALDMAA
                     TLAVPFTGDGRDAAEILRTLPPLK"
                     /name="tyrosine-type recombinase/integrase"
     gene            complement(17571..17759)
                     /locus_tag="KQ260_RS25525"
                     /old_locus_tag="KQ260_25480"
     CDS             complement(17571..17759)
                     /locus_tag="KQ260_RS25525"
                     /old_locus_tag="KQ260_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001332784.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPNSPKNGKPALSPHHDWLK
                     TGSLRQNAPLRIWFYPQ"
                     /name="hypothetical protein"
     gene            18126..19295
                     /locus_tag="KQ260_RS25530"
                     /old_locus_tag="KQ260_25485"
     CDS             18126..19295
                     /locus_tag="KQ260_RS25530"
                     /old_locus_tag="KQ260_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001072358.1"
                     /translation="MNQKWKTLIVSVLTPSSIISGIALIVLWGYFSRLDRLDIFFDVMN
                     IKSILVLVCCTTILSLIMIIFIFFITSFFIPIVIPYDIKNLPAYNKIQGNFLSVMMLSG
                     LFPMAFIYVLYCAFDFDQSVKDNSGWLSILSIGVLIVVISAIINTRYLEYDLSFKNNRM
                     KLLRRVQIYLIIPLSIALLVHLQVIPLEMVFRNIETSDKSINFKVIAELAFMSYFIFIL
                     TILPGLIYLKMNPQHKLSKRISSAFVASLMILLVISTQITVLPVIFTHSVIKLSGISDF
                     KIHSYIIKTSEYPEEFFSNAVWGKKNIKPGEYYSVQAVSMFTTNQFILLCPKDIIRFYR
                     ESWKFDLLNVDFDTNTRKKLQEEAAYCVPISAISVKRWDMPLQGSKPSN"
                     /name="hypothetical protein"
     gene            complement(<19521..>19696)
                     /locus_tag="KQ260_RS25535"
                     /old_locus_tag="KQ260_25490"
                     /pseudo=""
     CDS             complement(<19521..>19696)
                     /locus_tag="KQ260_RS25535"
                     /old_locus_tag="KQ260_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(20142..20414)
                     /locus_tag="KQ260_RS25540"
                     /old_locus_tag="KQ260_25495"
     CDS             complement(20142..20414)
                     /locus_tag="KQ260_RS25540"
                     /old_locus_tag="KQ260_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="WP_001322642.1"
                     /translation="MTPSPYPRNNFNTELIQSCMNGEHFSLLIEISPIRSKKNIMALKE
                     YLVDGYSKQESCERNNVSISYFCLCLKKLNYTHSIVSALKYFYRE"
                     /name="adhesin biosynthesis transcription regulatory family
                     protein"
     gene            complement(<20951..21375)
                     /locus_tag="KQ260_RS25545"
                     /old_locus_tag="KQ260_25500"
                     /pseudo=""
     CDS             complement(<20951..21375)
                     /locus_tag="KQ260_RS25545"
                     /old_locus_tag="KQ260_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            21657..23627
                     /locus_tag="KQ260_RS25550"
                     /old_locus_tag="KQ260_25505"
     CDS             21657..23627
                     /locus_tag="KQ260_RS25550"
                     /old_locus_tag="KQ260_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin/transferrin/lactoferrin
                     family receptor"
                     /protein_id="WP_001298664.1"
                     /translation="MYMNVIRNVICTLIILPIGLQAETSHSSMAKDTITVVATGNQNTV
                     FETPSMVSVVTNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVLVL
                     VDGIRQLSDMAKSSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADFLPP
                     GETNGLSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAPNKEK
                     PAALFAKGSVGITDSNKAGASLRLYRNNTTEPGNPTQTHGDSGLRDRKTVQNDVQFWYQ
                     YAPVDNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFPGAHQLS
                     YGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSYPVNIIVGSRYDRYKSFNPR
                     AGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRKGKPNYWVP
                     NLNLKPENNITREIGAGIQLDGLLTGNDRLQLKGGYFGTDARNYIATRVDMKRMRSYSY
                     NVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTLISDISIPVGH
                     GGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEASVTLDNAFNKLA
                     MNGKGVPLSGRTVSLYTRYQW"
                     /name="TonB-dependent hemoglobin/transferrin/lactoferrin
                     family receptor"
     gene            23634..24425
                     /locus_tag="KQ260_RS25555"
                     /old_locus_tag="KQ260_25510"
     CDS             23634..24425
                     /locus_tag="KQ260_RS25555"
                     /old_locus_tag="KQ260_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="WP_000977394.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNITAEIGYGHNF
                     PSKGTIPDRRNFFENPRIYNGKETITLKPASTDYVYKTESASKDNGYVLSTYMKPGYWS
                     RTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGHELEIIPLSDIS
                     RFSEDVKLKVLYKTSPLAGAIMELDSVSYLKSSRHTHAVEHKHPVHKAELTFVTNEDGI
                     VTVPSLHIGQWLAKVQNKKSFQDKSLCDETVDVATLSFSRN"
                     /name="DUF4198 domain-containing protein"
     gene            complement(24488..>25096)
                     /locus_tag="KQ260_RS25560"
                     /old_locus_tag="KQ260_25515"
                     /pseudo=""
     CDS             complement(24488..>25096)
                     /locus_tag="KQ260_RS25560"
                     /old_locus_tag="KQ260_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /name="IS66 family transposase"
     gene            complement(25164..>25878)
                     /gene="istB"
                     /locus_tag="KQ260_RS25565"
                     /old_locus_tag="KQ260_25520"
     CDS             complement(25164..>25878)
                     /gene="istB"
                     /locus_tag="KQ260_RS25565"
                     /old_locus_tag="KQ260_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="WP_097737038.1"
                     /translation="SAAPALSQQAVDQEWSYMDFLEHLLHEEKLARHQRKQAMYTRMAA
                     FPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLAIAMGYEA
                     VRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQEEAKL
                     FFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRILHHSHVVQIKGESYRL
                     RQKRKAGVIAEANPE"
                     /name="istB"
     Typing          15491..16467
                     /name="IncFIB"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.751"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagcacggcg acatccttta ccggaaaaga aacgggcggt tgcccgcccg tctccgtcat
       61 acttcatccg ccataatgcg ccacattgtc gactatccac gcaggtccgg tgacttccgg
      121 cagctcatcg tcatctgtcg gggaagacca ttccagttcc ccccacagca catcaacaag
      181 ctctccgcgc tcatacaact gccagctgtc agcgccagtg atataagacg gtaattcacc
      241 atttggattg tccgctccac ccaacatgtt gtttccttaa ggttctcaca ccagaaagga
      301 catcaacatg ctgagcagag aggactttta catgataaag caaatgcgcc agcagggcgc
      361 gtacattgtc gatattgcga ctcagattgg ttgctctgaa cggacggtca gacgctacct
      421 caaataccct gaaccgccag ccagaaagac ccgccacaaa atggttaagc tgaaaccgtt
      481 tatggattac atcgacatgc gcctggcaga gaatgtctgg aatagtgagg ttatctttgc
      541 ggagattaag gcaatgggtt atacgggcgg acgttccatg ctgcgttact acatccagcc
      601 caaacgtaaa atgcgtccgt caaaaagaac agttcgcttc gaaactcagc ctggatacca
      661 gctccagcac gactggggcg aagttgaggt ggaggttgcc gggcaacggt gcaaagttaa
      721 ctttgcggtt aatacgctgg ggttctcccg ccgcttccat gtcttcgccg caccaaaaca
      781 ggatgctgag catacctacg aatcactggt tcgcgccttc cgctacttcg gtggttgtgt
      841 gaaaacggtg ctggttgata accagaaggc tgcggtgctg aagaataaca acgggaaagt
      901 cgtgttcaac tccggattcc tgttgctggc cgaccactat aacttcctgc cacgggcatg
      961 ccgtccacgc agggccagaa caaaaggtaa ggttgagcgg atggtgaaat acctcaagga
     1021 gaacttcttc gttcggtacc gcaggttcga cagcttcact catgttaatc aacaactgga
     1081 gcaatggata gccgatgtgg ctgacaaacg ggaacttcgc cagttcaaag aaacgccgga
     1141 acagcgcttc gcgctggagc aggaacatct gcagccgtta ccggatacgg acttcgatac
     1201 cagttacttc gacatccgcc atgtgtcccg ggacagctat atcgaggttg gtggtaatcg
     1261 ttacagcgtt cccgaagcgc tgtgtggtca gccggtatcg atacgaatat cgctggatga
     1321 cgagttgcgg atctacagta atgagaaact ggtggcctca catcgcctct gttcggcatc
     1381 gtctggctgg cagacagtgc cggagcatca cgccccgctc tggcagcagg tcagtcaggt
     1441 ggaacatcga ccactgagtg cctatgagga gctgttgtga tgcatgagct ggaagtcctg
     1501 ctgagtcgcc tgaaaatgga gcatctgagt tatcacgttg aaagcctgct ggaacaggca
     1561 gctaaaaaag agctgaacta ccgggagttc ctgtgcatgg cgctacagca ggaatggaac
     1621 ggcaggcatc agcgcggtat ggagtccagg ctgaagcagg ctcgtctgcc gtgggtcaaa
     1681 acgctggagc agttcgactt taccttccag ccgggcatcg accgtaaggt tgtccgggaa
     1741 ctggctggtc tggcgttcgt ggagcgcagc gaaaacgtga tcctgctggg acctcctggt
     1801 gtcggaaaaa ctcatctggc catagctctt ggcgtgaaag cggtggatgc gggacatcgg
     1861 gtactgttta tgccactgga cagactgatc gcgacactga tgaaagcgaa acaggaaaac
     1921 cggctggagc gtcagctgca gcaactgagt tatgcccggg tgttgatcct ggatgaaata
     1981 ggctatctgc cgatgaacag agaggaagcc agcctgttct tccggctact gaaccgtcga
     2041 tatgaaaaag cgagcatcat actgacgtca aacaaagggt tcgcagactg gggagaaatg
     2101 ttcggagatc acgtgctggc aacagcgata ctggatcggt tgctacatca ctcaaccacg
     2161 ctgaatatca aaggagagag ttaccggtta aaagagaaac gtaaagctgg agtgctgacc
     2221 aaaaacacaa cgccaatcag tgatgatgaa atggtgaaaa gcggacagca tcagtaacga
     2281 aagtatctta gcgggcatga aaatggcaaa taacggtcaa acatcgtggc gttgacacag
     2341 ccacagaaat tgcagccctg ttcggcgtac cagtgctcca gcgtgcagcc gaaacggcgg
     2401 cttaattctg caacaacgtc gctttccggc tgacaccacg gcgtgtcgaa atctacctga
     2461 atgaagtttt ccccctgact gctgatattc aggtcatacc cacaaggcca cttcacgcca
     2521 taccgttctg tataccagtg atatgcagaa ggaacaccgt tcagaacacc gccgttaaag
     2581 ccattcactt caatatccag gcgggtcggc aaaaccgtca gcaggtcaaa cggcatggtt
     2641 ttttccgaca gtacgttgtc acacagacgg ttccaccaca ccgatacatc ctcgttaccc
     2701 cagatgtcac accagtcacc tctttttgcg gtgaaatgca cagtgatagt ttccctgaca
     2761 tcatccggta acccttccca gcgacgctgt ccagtaccgc tctgcagcca gagttcatgc
     2821 agcatcaggc agttttgctc atccagcagt acaccgtcct gcaggtgctt cagccagcgg
     2881 gtgaacgtga tattctccgg cgataaaaca ccacgtccgg cagccgtcag tccggggcat
     2941 ggttcaaacc gcacatcttc agtggtctgc agaagtccgg cacttccggc cagaaacagc
     3001 tgaatacctt catttgtggc gcggcgataa aacggtgtga ctgcaccgct ggccagccgt
     3061 ttaatctcag caatctttgc cggttcacca gaaaaataca tacgattcga gcaccagttt
     3121 ggcatgggaa ttctcctctc acatacggcc tccatcaggc cgtgccgcac ctgtaaccgg
     3181 gcgaccgcat acgaatgcag gtgcggcccc gaagccgcag cgcgtggagg aggaacgacg
     3241 aaacgtgacc gggtgaggag gccggcgaca gcgacacact tgcatcggat gcagcccgat
     3301 taacgtgccg gcacggcctg ggtaaccaag tattttgtcc acagaaccgt gcacaaaatg
     3361 ctgtggataa gctggacaca gcagtccaca gcaggcatac agccacaccc tcagagaatt
     3421 gagcgttttt tagtcacaat aatgacattt gactgtgtgt ggattcagtt attagaatac
     3481 tgtatataca tgggtcttcc ccgatcatgg tgggaagact cagtacgcca tattcagttt
     3541 cccatagtgg aacataactc ccagtttaaa gcgttcccgg tttcggtatc ccctggcctt
     3601 tatcctcagc agtctgatct tgctgttcag cgcctccgca tttccattcg agacgcgatg
     3661 acgcattgca ttcagtattc cgtataaccg ttttttaatg gttttcgctg cgtttttcat
     3721 catcggcaca tcacattcag acgcccgcga tatccactgc aaccagtcat tcctgcgttc
     3781 tgtgctccag gggcgatccc agatattttt tgccagctct ttcagtgtcc agcactggct
     3841 tgtctgttgc atctgttccc gcaaccacat cagcttttcc tgccgggact cagtcatcca
     3901 tttgtcgctg tactgccaca ggaagcgggt acctttggcc tgacgacgac tttcaacagg
     3961 gaggtgcgga tgttcattct ggcgggtttt atcaaccact tcgcccagtt gcttcgccac
     4021 atggaaacga tcgaaggcga ttttctcgac cgcattgggt aaatggatac gcgctgctct
     4081 tatatagccc gcattcatgt ccatcgacag tgttttgatg gccaacaact gactgtcagt
     4141 aagcgaacgg agatagctgg caagactctc tgtgccacga tcatcggtta atgccagtgc
     4201 ccgcccgtcg cgatcagata ccacggttat atacctatgc ccttttttaa aggcgacttc
     4261 gtctacattc atatgacgca ctgataaagg ctttttaatc cgcgacaggc ctcgcttaac
     4321 cgcgcgggtc atgatgccgt caacggcatt ccagctaagt ttaagttgtt ttctgaccgc
     4381 atcaacggtg ctgattttaa gccatgagag cacgaacgat tcgaacagca gagtgtaccg
     4441 actgccggaa cctgcccacg gtacaggcag agtctggcag ccatgctccg ggcacataac
     4501 gcggggaaca tcggcttcta ctaatgtcat gaactggcag gtatcaaggt ggcgccattt
     4561 acgatgtcgg tgatcgtgaa cagaacagga tttcctgcag gtcggacagg ttaactgagt
     4621 attttcagca attccgactg taacagtaac ggaacctgca ttttcatcga gggtaaggga
     4681 tttaacctgc catggtgcag tcaggttaag gatatgggca tagagggatt tttcgtccat
     4741 ataatttcat tctgaagctt actttaaact gatattatta ttttttgccc actttcttaa
     4801 tcttccatta gcgttggcgt aaggcgaaga tgtgttaaat gagatcattc tccccatcgt
     4861 ccattttgtg taccatggtc ttccatacaa aatatcatcg gtacgatcat taataaggtt
     4921 cacaatctca ttttttacag tctgaagttc agctactaac aactcataac ttaactcact
     4981 gtaatctgag taaaattttt gagcaagaag gccaagctga ttccatttat agccagtttc
     5041 cggaaaatcg acaggcagac ctttagcatc agaagcgatc cactttacaa caagagcatt
     5101 ccatccaagc agatacgaaa cgagatcacg aacactcatc tccgttcctt tggcgtgtcc
     5161 gtccattgat ttatctgaag taatttctgg tgggattgtg ttgaggtaac taattaattt
     5221 actaaaattt ttatcaatag ctaaaagcag ttcagctttt gtttgcggca cactcataag
     5281 aattccctct catgtaaaaa gagacaccat aaaggtactg tgtggtgttt gaaaatcact
     5341 caattatgta ccacccacca caacagggga agacccatat acatacagca aaagaaaagg
     5401 gagatgagaa catgatccgc attgaaattc ttttcgaccg ccagagcaca aaaaacctca
     5461 aatcaggcac acttcaggcg ctacagaatg aaatcgaaca acgtctgaaa ccacactacc
     5521 cggaaatctg gctgcgtata gaccagggca gcgccccgtc tgtatctgtc actggagccc
     5581 gcaacgacaa gggtaaagaa cgtatccagt ctctgctgga agaaatctgg caggacgaca
     5641 gctggctgcc cgcagcatga gtaccgttta tcaccgtccg gctgaccctt caggcgatga
     5701 cagttacgtg cgaccgttgt ttgccgatcg ttgccaggcc ggttttcctt cgcctgccac
     5761 tgattatgct gagcaggaac tggatctgaa cagctattgc atcagcagac ctgcagccac
     5821 cttctttctg cgcaccagcg gtgaatcgat gaaccaggct ggcgtgcaga atggtgatct
     5881 gctggtagtg gacagggccg aaaaaccaca acacggggac atcgttatcg ctgagatcga
     5941 cggtgagttc accgtcaaac gactgctgtt gcgcccacgc ccggcactgg agccggtttc
     6001 agacagcccg gagttccgca cactgtatcc ggaaaacatc tgtatttttg gtattgtcac
     6061 tcacgtgata cacaggacgc gggagttacg ctgatgtttg cactggctga tatcaacagt
     6121 ttctacgcct catgtgaaaa agttttccgc ccggaccttc gcaacgaacc ggtcatcgta
     6181 ctcagcaata acgatggctg tgtgatcgcg cgcagcccgg aggcaaaagc ccttggcatc
     6241 agaatggggc agccctggtt tcaggtgaga caaatgcgcc tggagaagaa aatacatgta
     6301 ttttccagca attatgcgct gtaccacagc atgagccaac gggttatggc tgttctggag
     6361 tcgctttctc ccgcagttga gccctactca attgatgaaa tgttcattga tttgcggggg
     6421 ataaatcatt gcatctctcc ggagtttttt ggtcatcagc tcagggaaca ggtaaagagc
     6481 tggacaggac tcaccatggg ggtgggcatt gcgcctacaa aaacgctggc taaaagtgca
     6541 cagtgggcaa caaagcaatg gccacagttt tccggtgtgg tcgcgctgac ggcagaaaac
     6601 cgtaatcgga tcttgaagct actggggctg cagccagttg gtgaggtctg gggagtagga
     6661 cgcagactga cggaaaagct gaatgcgctg ggtattaaca cagcactgca actggcgcag
     6721 gctaacacgg cattcatccg gaaaaacttc agcgtcattc ttgagcgtac ggtacgcgaa
     6781 ctcaacggcg agtccagcat atccctggaa gaagtaccac cggcaaaaca gcagattgtc
     6841 tgtagtcgca gttttggtga acgaatcaca gacaaagatg ccatgcacca ggctgttgtt
     6901 cagtatgcag agcgggccgc agagaaacta cgtggggagc gtcagtattg tcggcaggtg
     6961 acgacatttg tacggacatc cccctttgca gtaaaagaac cctgttacag caatgccgct
     7021 gtggaaaagc tttcattgcc acacaggaca gccgggacat tattgccgcc gcatgcagag
     7081 tcttaaacca tgtctggcgt gaagggtacc gctatatgaa ggcaggagtc atgctggctg
     7141 atttcacacc atcgggtata gcgcagccgg gattatttga tgaaatccag ccccgtaaaa
     7201 acagtgaaaa gttaatgaaa acactcgatg aactgaacca gtcgggaaaa gggaaagtgt
     7261 ggtttgcggg gcgaggaacc gcccctgaat ggcaaatgaa acgggaaatg ctatcgcctt
     7321 cgtatacaac acaatggact gagatcccca ttgcttcatt ctgatatatc agaataattt
     7381 tttcatttta agacgcacat cattcattgc ctcctgcacc gaatcagtag cagaaggagg
     7441 cattttttca acagattgcg aagcaccgag ttcagtcaac acagttctga tatcggcctg
     7501 agaaggcttc catccagtcg tctgactgag taaggttaca acctgatctg ctgaaaactc
     7561 ctcgctcaaa atggctgtca gcaatacagg caggcgagga tcaagttggt gtaacgccat
     7621 tccagaaata aatgcattcc ggaacagctc accacgctca gaacgcggaa cggactctat
     7681 cgcttccaaa gtttgaaagt ctgcagcttt ttccggatgc agataaagtg tgtatttttt
     7741 ccttttccgt tcatcgtcca caatattact cctctttgaa agccgcgata gcttcaacca
     7801 gtgctgtctg tggcgactcc atcatcacta ctttttgccc aagatggtgc catgccgttt
     7861 ttatcgaatc atagatcaac ggggccccac caccgacaac ataaacacga ttcacatgat
     7921 ggaaatcacc gagctcattg actacttgct cccccaaact ggcaatagcc ccctcaatgg
     7981 tattaaggac cagatcagtt tttgtttcgt catttataac ctgccgaaca aagtccgggt
     8041 cattacgacg tttaatcaat tcatctgcaa cgaggaaact ggtatcactg gatgccatgc
     8101 gcaatgcact cattgcagct ttcgtaactg aagacacacc aatgccagaa ttaccatgaa
     8161 tggcacttac agagtcaaat tgaccaacga taacaccaac atcaagcgta gtgccgccaa
     8221 tatcaactac cagcgatttt tcgaactgac caactttatc cacgaccaga cgagaaaaca
     8281 cagctggcaa tgattcaggc atcacatcaa cgtgttcaat ggtgaaaaca tcgcctttat
     8341 taagacgaat cggcctcatt aaattctcaa ttttacgctg aatgttgagt tcattttttt
     8401 gacactcttt cgtatagaac tcactgatag gcaaagtcac agtcaatgat acaggctgag
     8461 gcgctaatcc actattcagt aaagcgtgat gcacagctaa caggttaaca tccgtatatt
     8521 gatactcaat atgagtcgta agaatagatt ggttgctaac ttcatcatac gtgtattttt
     8581 taccatccag ttcatagtta aatgtctggc gaattcctaa cccgtcaacc ttccatccat
     8641 gacgaaatga gttttgcgac aacgacttac agattttccc attatcattc catgccaact
     8701 tgattgttgt tgaaccatca tcgcagtata cgttcatcta tagcccctta aatactcaat
     8761 ctgagtaaat cattgtttct ggttgcaagg taatactcaa tttgagcacc atcatagtta
     8821 tgaaatcaca aaacacaagt attatttatt acatcacatg gcacacttca catttcatac
     8881 ttcataaata tgaacacata ttgacacctc aggttaccgt gtcaaaatat catcatactt
     8941 cacacatcac atttatgagg ttgcaatgat tactgtagtt ggtgggaaca aaggtggttc
     9001 agggaaaact accattgcat caaacctggc tattgctctc gcaaataaag gaagggaagt
     9061 ctgcctgtta aacggagatc tacaacgaac agccgcaaaa catcatgcgg aacgagaagc
     9121 tgcaggactg ttaccagcaa taacccttgt tgagaaattt gataacctga cacaaacact
     9181 acaggctctg aatgaaaagt tcgatgatgt aatagtggat gtcgcaggaa gaaactcaaa
     9241 ggaattcata acgagtggag ttgtcgcaca tcaaataatt gcgccactac agtgttctca
     9301 accagatctg gatactctta cagaactaga acaacagata gacgcaatgc gtaaccttaa
     9361 cccggaactt aaggtttatt gtttacaaag tatggcaaca accaatccgg ttttgagggg
     9421 aaatgagaga aaagaattcc ttgaatatct tgaagaattc ccaacaattc aagtccttga
     9481 ttcagttatt tgtttcagaa aagtctatcg cgattgcatg agcaacggca ccggtgtagt
     9541 agaaacgaac aatacagcag ccaaagcaga aattgaacat ttaatgaatg aggtatttgg
     9601 cccatggtga agaaacccag ccagcaagca ttaaacagag ctgcagttac ggtagaacaa
     9661 gcagaggctc tggctcagcg ccttgcggat aaaccatacg gcgcgccgga aaaacccgaa
     9721 ccagaaaaac aatgtcggac gacaatctct cttggtgaaa gcatgcttgt cacaatagaa
     9781 gatctggcat tgaggaataa acgcaatggt aaagatccaa aaaatgtaag tgccatcgtc
     9841 agagtagccc ttgagcaata cttaaaaaca ctaacgtaat aatcaataag ttattactta
     9901 tgaaatcaca cttgtgaatt catactgttt aagcaccctt gcatacaaga gatcacatat
     9961 tgcagtggtc tcttttttta cagaataaat cacaattcat aattatgatt tatgaattat
    10021 gcgtgtctgt ttttcagcat tgtgaccgca tcggactcag gcatcgaaaa ctgcacccgg
    10081 tgccgtgcag ccacatccag cgcaaacacc ttcgtgtaga cctccgttga gctgatggac
    10141 ttatgcccca tcaggctctg caggaccttc agtggtatac cggcatacag catgtgcatc
    10201 gcataggaat ggcggaacgt atgtggcgtg accgggacag agaacgtcac accgtcagta
    10261 gcagcggcgg caaccgcctc cccaatccag gtcctgaccg ttctgtccgt cacttcccag
    10321 atccgcgctt tctctgtcct gcctgttcgt ttattacgcc gttccagagg aattttcagt
    10381 gttgccacca tcgtctgcag ctgactgacg taccaggagt cggagagcgg aaccagccgg
    10441 tgagtctgct gtccggcagg cgttcttcct gccgtcctgg cggctttttc ggtccgctgc
    10501 ttcagagtgg ccagctgcac aaacggatac ggaggcgcga gcgaaaaatc cccccgcgtc
    10561 agcgccagtg cttcattaat gcgcgcaccg gtgttccaca gtgtggccag cagcatcttg
    10621 cggcgcagat ccgggacgta atggagcagg gcactcactt ccggagccag cagatatttc
    10681 ggcagttcat catgaaccat cgacatctgg cgaagtgcca gagctgccgg ataatcaatg
    10741 acaacaggaa gttgtggaga ctgtccggca gaataaactg ccggtaccct ggctgcagac
    10801 tggctgtgta taacggagcc tgacatttat attccccaga acatcaggtt aatggcgttt
    10861 ttgatgtcat tttcgcggtg actgagatca gccacttctt ccccgataac ggagaccggc
    10921 acactggcca tatcggtggt catcatgcgc cagctttcat ccccgacatg caccaccggg
    10981 taaagctcac gggagacttt gtctgacagc aggcgggcac tggccagggg gatcaccatc
    11041 cgtcgcccgg gcgtgtcaat aatgtcactc tgaacatcca caaacagacg ataacggctc
    11101 tctcttttat aggtgtaaac cttaaactgc atttcaccag tccctgttct catcggcaaa
    11161 agagccgttc atttcaataa accgggcgac ctcagccatc ccttcctgat tttccgcttt
    11221 ccagcgttcg gcacgcagac gacgggcttc attctgcatg gttgtgctta ccagaccgga
    11281 gatattgaca tcatatgcct tgagcaattg atagctgtcg ctgtcaactg tcactgtaat
    11341 acgctgcttc atagcacacc tctttttgac atacttcggg tatgcatata agtatatatt
    11401 attatgatgc aaaaatcagc gcgcaaatac acatactgtt atcttacttt tagtaagcct
    11461 tatttatttt accttgcgtt atgttaacca ataaaaatca aaatctggct tataaaaaca
    11521 aaccgtaatt atcgcattcc cgtttccgtc agatccgact ttcattaata tcacaacttt
    11581 ccggaagtgt accacgctga gaaaatcatc atatcactgt tttccgcccc tgcgcggcgc
    11641 atcacaggtc agggagatgg cacaacatat agtgtcatta acagctaaat tcagcactac
    11701 atgttgtgct gacgggagat ggcgctgtgc ggcgggagag gaagatccgt gactaccttt
    11761 tttcggatct tattagtgta gaagaacgat tgttgcggtg aggggattat tgcttaggaa
    11821 gcaaaattca aaactgacta agccagaaat agcaacaaca agataaggaa attttccccc
    11881 tcagcccctg catgccgtga gggggagcaa ctacccaact acagttttca ttttatgata
    11941 cgtcttcact aggtaatacc tgtgaactga cagagtcttt acggagtgta tggcggacct
    12001 aaatttgatc cctgaaaact gtcagcgctg cgaggacgaa gaaagtatta cggctaaagc
    12061 cgtaatacct ccccatccag cttcaagcta tgtagaaact tatgtgggta gagcagtccc
    12121 gacagggcag taccgcattg acaggccgtc agggccgctt acgagcttgt atgacaaggg
    12181 gggcctagcg aattattgta cggtaagcct tttttgaaca gccttaccta ttgctataga
    12241 ggggaactcc tacgttatac gccgctttca ggggattcga ccttaatgag acaactcaaa
    12301 tatgatcgac aacttaaacg cgatagttgc tgataaaatc aatccattac gcgtttcaat
    12361 tgattaaata atgcacttaa tgtacaaaaa ttcccgatct caaaactgaa ccctaaagat
    12421 accaggcatc tcccccagga agcgccctca tgcgttttcc tgttccgccc ctgccaccta
    12481 ccttctgtta catacttgtt gtattattgt ttttgttttc aatccccaag gagacgtcgc
    12541 tatatgactg tgcgggagac agaggaaaac attgaacaaa tcttccctga gctttcgaac
    12601 ttaattgata ttattaataa atggctttcc catggaaaca ttgatcctga actggcacac
    12661 ttcatcattt atcttcttga ttacctaacc accacacaca aaagcatata aaaaatttcc
    12721 ttgcacatcc atttttttgc gaatattatt attcctgctg aatatgattc aatatcgcac
    12781 gctactcatc catcccagga taatgagttc ataggttgaa gtctcaacac ccccactacg
    12841 ggggtgtttt ttttaccaat aaacacaaat cacaccatcg ccatactctg tttgccagac
    12901 atcgattggt aaatatgcgt aactcgctat aatacaaaaa gaaaaatcag aaccagaaac
    12961 cagtttccag ataaaaaaca ccctcccgaa ggaggggaca gacacattca tatttcccaa
    13021 aacaggaatt tttattttta tgacgcatta atgaagttac ttcatctcca gtagattaac
    13081 ttcatttaaa gtggcgatca atcatattta ttacgactct aaactattac aacaaccaca
    13141 ataagatatc acctcaaata taaaagataa ttcttacaaa aacgttccga aaacaaaaaa
    13201 tacagataaa caccaattaa ttcagagcaa tctattgcga agaatcagag taacagggcc
    13261 ccatgatata atcatggggc cctgttgctt ttacagatgt aaaaacgacc aatcatttaa
    13321 catctgttat cggcaccgcc ccggacgggt tcggcgcaaa tatggtatgg taccagcccg
    13381 gcaattcacc tgccgtgcaa tctgacgaag cagtaccggc gtatgcagat atatcgtcca
    13441 gacatatctg ccgcattcag gcggtgagta aagccttctc tctcaaaaga ggagcaacag
    13501 ttatccacag attctgggga taactgcgaa aagaatatgt gccaggaaag tcggctcccg
    13561 ggaaaaagac cgctggcggc gcacctgctg gatacgaaag ctgagctgaa aggatcccgg
    13621 gaggacgtcc gtaaacttca gtccctggcc cgtcatgacg ggaaggcgaa aaagtgaatg
    13681 cggacacctg gacccgcctg caggccttcc gacatgctct ggagctgact tcctgtgagg
    13741 cttctctcac cgccgggtat gatcacctga aggatttccc ggccggttgc agcgagctgg
    13801 ccagccagac gctgactgat tatctgacgg aagacggcag caacctgtac tcctgtattg
    13861 tcggtatgca gtgggataat ggtcctggca gatacggcca cgtcattgcc gaccctgcca
    13921 gggactatat cgacctaacc cttgatcagt ttccaggcta tcataaccgg attgtggcag
    13981 agcccgttga gtccggcgga cagctcgcgg cagacctcaa cagggagcct gccatcagta
    14041 cagctgatgg aatagtggcc agcccatcag ataaggttaa ttacattact gaacagaaaa
    14101 atcgccagcc aatggtgatc attacagaat gttacaagct gatcagaatg acggcaagat
    14161 ttataccaat gtgccagtca tctcaactct tccagcacca ggtattccct cgcttattta
    14221 tcatttcaga cgttggctcc tgcagtttgt tcaggaacac cttagatcgt ttagctactg
    14281 actggaaggt gacactgtcc atcctgtcgc tatagggctt gatataaacc tggcaggcaa
    14341 gggatctggt taactccttt ccttcaataa agtgtttgaa gatccagaga tacgtatcca
    14401 caagctccag ccctgtactc tgtgtccccg actggaagac ctgcggttca gctggtattt
    14461 ccattacatc catgaccggt gtctgcgcag cagtgcctgt catcaggatc ctgtcactct
    14521 ttcgaccgac agattatggc atacagttgg gctcgtaaga tccagagtca ataactgaca
    14581 gggtttattc tgtcatcctc cggcagcaaa ggcatatacg atattcactt attttgtcgg
    14641 tccaacggct ttttacagta gctcgttttg cccaactgat gctacagctc tttggtttgc
    14701 ttcatcacgg atatcagctc cgacacaggc acagctgact ggttgcaacg tctgcacttc
    14761 tctggacctg aaacgtcaat tatcaggatt ctgacgatta gtcttaagac tgtcggtaaa
    14821 ttaagtgatg ccagttgtca ggatattcat ggagccagtc tgcatgcgcc tctggcgttg
    14881 agacaaacat atgaagtgta tttcggatat gactgtacgc tcaggaagta cggcagattc
    14941 cgtcaaaaat tacctgtaaa gacagacttc agaaaaaaca gtgctcgccg cagaccggtg
    15001 accgggcgaa gcccgcgaac cggcgaggaa gcgcaatata tctgtagtta cagtgagcag
    15061 gtacgccctg aagccttcat aatcggtagt gtatgttgtt tttccggaat gatttatcca
    15121 catatccaga ggcccgatcc agtaaacaga tccatgaatg atcaacaaag gatccattaa
    15181 agatccccat atcgctgcaa accttgtcac tcatgggccg ggaccacgtt cacataagct
    15241 gtggcatgtt atggataaac tgtaacaggt tcatgataag ctgtattcag taatgcatat
    15301 actgaagtaa gttaatgaca taaactatgg tcagtacgcc agactcagtt gttaaataca
    15361 gactgcaggt ttttcttcag tcagttgacg gggttctgac acacgatttt ctgtttattc
    15421 ttttactgtc cacaggcagg aggctttctg gaaaacgaaa attcagacat caaaaaactg
    15481 ttcggcgagg tggataagtc gtccggtgag ctggtgacac tgacaccaaa caataacaac
    15541 accgtacaac ctgtggcgct gatgcgtctg ggcgtctttg tacctaccct taagtcactg
    15601 aagaacagta aaaaaaatac actgtcacgc accgatgcca cggaagaact gacgcgtctt
    15661 tctctggccc gtgcagaagg attcgataag gttgagatca ccggcccccg gctggatatg
    15721 gataacgatt tcaagacctg ggtggggatc attcattcct ttgcccgcca taacgtgatt
    15781 ggtgacaaag ttgaactgcc ttttgtcgag tttgcaaaac tgtgtggtat accctcaagc
    15841 cagtcatcac gcaggctgcg tgagcgcatc agcccttccc tgaaacgcat tgccggtacc
    15901 gtgatctcct tttcccgcac cgatgagaag cacacccggg aatacatcac ccatctggta
    15961 cagtcagcct actacgatac tgaacgggat attgttcagt tacaggctga tccccgcctt
    16021 tttgaactgt accagtttga cagaaaggtc cttcttcagc ttaaggcgat taatgccctg
    16081 aagcgacggg agtccgccca ggcactttac acctttatag agagcctgcc ccgggatccg
    16141 gcaccgatat cgctggcgcg gctgcgtgca cgcctcaatc tgaagtctcc tgtattttcc
    16201 cagaaccaga cggtcagacg ggcaatggag cagttgcgcg agattggata tcttgattac
    16261 acggagatcc agcgggggcg gacaaaattc ttctgtattc actaccggcg tccccggtta
    16321 aaagcgccga atgatgagag taaggaaaat cagttgccac cttcacctgc ggaaaaaatc
    16381 agtccggaga tggcggagaa gcttgccctg cttgaaaaac tgggcatcac gctggatgac
    16441 ctggaaaaac tcttcaaatc ccgctgaaca taaactgtag tcagataaga gagactcttt
    16501 actgactaca gtttatatta acaggtgcag tgagtgatgt gctcactgta gtttatattc
    16561 agtttcctgc tgcactgtct gtggctgagc tgccatctgc ctgttcctta cataaaacac
    16621 ggcttactca ctacagttta tctacctgct atagcttatg ttatgcctgt tctgcttacc
    16681 tccgcttata taaggaagag cgtatgctgt cacttcaggg gaggcagtgt acgcaggatc
    16741 tctgcagcat cgcgcccgtc acctgtgaaa ggcactgcca gcgtggcagc catatccagc
    16801 gcaaaaactc tggtataaac ctccatcgaa cgtggatccc tgtgaccagc cagtgcctgg
    16861 atgactttcc ggggctggcg gtgatagagc atgtgcatga tatagctgtg ccggaaggta
    16921 tgtggtgtga ccggaatcga aaaatgcacg ccgtcagctt cagcccgctt caccgcctgt
    16981 ttcagccagt tgcgcattgt ctcatccgtg accggccata acggctcccg ccggcggggc
    17041 cgggtggtga tcatccagct ttccatctgc ctgacatagc ttatatctgt cagcggaacc
    17101 aggcgcactt catcttttgg cgggcgtccg cgtcgcgcac gcactttttc ggacaggatc
    17161 cgcacaaacg gtcttactcc atccaggtca aatgattccg gcgtcagcat ccgggcttcg
    17221 ccaatacgca ttccggtatt ccagagggtg gcgaacagca tatggtgacg ctgatccggc
    17281 atatagaaaa gaagggcact cacttccggg gccagcaggt aggccggtgt ggcccccgtg
    17341 gaagtggcca ttcttctcag ggagagcgct gttgcaaaat ccacccccgg cgcaatgggt
    17401 aacagggaga cgcgttctgg tgaattctgc aggggaatga cattgttcat gagttatttt
    17461 tcacgtagca cacgcactgt ttatggatac aggtattctg acctgaacgc ccggatcctt
    17521 ccacgacaat aaatcgcagc gcgcggatcc ttttgtttga ttatcacgca ttattgcggg
    17581 taaaaccaaa tgcgtaaagg cgcattttgc cgcagggatc ccgtcttaag ccagtcatgg
    17641 tgcgggctga gagctggttt accgtttttg ggagaattgg ggtaatgcca ggaacaggca
    17701 gatacggaga aaaatcctgc agataacctt ctacgacaac tgaaggtgtc tgacgccata
    17761 ttcttagcta cttctcagtt ttaaggcggc ttagtcacaa cataaggtta tgcataataa
    17821 gcatatttta ttatgttatg ttatggtctt gttcatgcat gaactgttat ctattcagtc
    17881 acttttaacg ttttatatca tactgcatat gacacaatca tcttctatat tcagattaaa
    17941 aatattatga cgacgatcaa ataaatttag tgaaatcatt acttacaata atatgtttat
    18001 gacataattt atcaaaaata tccgacaacc ttcctgaaag gttactgcga ggagtcaaac
    18061 aatgaagtgt aataacttat tcactctttt ttatgctctc tttatagcaa aaccaggaag
    18121 ttaaaatgaa tcagaagtgg aagacgctaa tagtttcagt attaacacca tcaagtatta
    18181 tatcagggat tgcgttgata gttctctggg gatatttcag tcgtctggat cgacttgata
    18241 ttttctttga tgtgatgaat attaaaagca tcttggtatt ggtttgttgt acaactattc
    18301 tatcattgat tatgatcatt tttatttttt ttataacatc tttttttata ccaatagtta
    18361 ttccttatga tataaaaaat cttcctgctt acaataagat tcagggtaat tttttatctg
    18421 taatgatgtt atctggcctc ttccccatgg cctttatata cgtgttatac tgtgcatttg
    18481 attttgacca gagtgttaaa gataactctg gctggttatc tattttaagt ataggagtac
    18541 taatagttgt tatttcagcc ataataaaca cgaggtatct tgaatatgac ttatcgttta
    18601 aaaataatag aatgaaactg ttgagacgtg ttcagatcta tcttatcatt ccgttgtcta
    18661 ttgctttact ggtgcatctt caggtaattc cactggagat ggtttttagg aatattgaaa
    18721 cttcagataa gagtattaat ttcaaagtga tagctgaact ggctttcatg tcatatttta
    18781 tttttattct gacaatactt cccggcctca tttatctaaa aatgaatcca cagcataaac
    18841 tttctaaaag aattagttct gcatttgtcg catccttgat gatattatta gttatatcga
    18901 ctcaaataac cgtattacca gtaattttta cccattcagt tattaagctc tcaggtataa
    18961 gtgacttcaa aatacatagc tacattatta aaacaagtga atatccggag gagttttttt
    19021 ccaatgcagt atggggtaaa aaaaatataa aaccggggga atattactct gtgcaggctg
    19081 tttctatgtt tacgacaaac cagtttattc tcctttgtcc caaggatatt ataaggtttt
    19141 atcgggaaag ctggaagttc gacctgctta atgtagactt cgatacaaat accaggaaga
    19201 aactccagga agaagcagca tattgcgtac caatttctgc aatatctgta aaacgatggg
    19261 atatgcctct tcagggctcg aaaccctcaa attagaaggc cgctcgtgcg actgtattag
    19321 atagtaatgc ggtatggaaa aatagtgatg gcaagatgaa cttgacttgg ctaatcaaat
    19381 accggcgtag tgattaggaa acagggctcg gataacatta ttgcgcggtt cattgaacct
    19441 actgatgacg ccggatatat agtcgtaaac cgtttcgaac cagtacttat atcaccagga
    19501 gagtccatat atgaccaccg tttacagtaa aaacggtatc tgttttctga tccctccagc
    19561 gtcaaactac cgttgtaatg gtgaggtctg gtgctattgt aataattcag aatataatct
    19621 ctgatttatt tccgaacctc gcccttgcct gcgtaaccat ccgtcgcccc catcctgttt
    19681 tcagactgcg gaaaaaggca agtttttatc cccccacaga gattcaatca tatatcgcgt
    19741 gttttggaag tgacagttct tattcctgag aatactgcat gtgcaacaca ctcggcatcc
    19801 atcgatacca cttttatctc caataggcag gataaaaaac aagactgtga attaaagtaa
    19861 tcggcaaatg aatgagttag aagatactga aaatgaatca aggactaacc ccgtagggaa
    19921 tgaaaataat aaaagaacat cttgttttta ttttaatgaa tgagcatgtt cttttatatt
    19981 aataacaaaa cctatcatta aacaaaatga tgaatgacag agttttcttt aaaatagctt
    20041 atattatctt acagtagctg acatagtgca atataaatta tcagctatgc cataacggct
    20101 tagcagtgtc taagcgtaag aatcaagact tcagttacag tttactcacg gtagaaatat
    20161 ttaagagcac taacaatact gtgtgtatag tttagttttt ttaggcatag gcaaaaataa
    20221 ctaatggaaa cattgttcct ttcacaactt tcttgctttg aataaccatc aaccaaatac
    20281 tccttaagag ccatgatgtt ttttttactt ctgattggtg atatttcaat taaaagtgaa
    20341 aagtgttctc cattcataca tgattgaatt agctctgtat taaagttatt tcttggatag
    20401 ggggagggag tcatatatta tctaacctta tttttctatt aaagaattga aatagaatag
    20461 acagaaatgc tattggagta agtatttgat agtaggtaat ttacgctcct atatagacta
    20521 ttgttactca tataatattt tggggtggct tatgttgtga cagcgccagt tgttaaaaac
    20581 gttgtaccgt catacgggat aatatagaac aaccaataac gattactggt ctatgaccac
    20641 cgccagagat acagaaaagc tttatctgta atgagctcaa ctatatatcc cgtcaactgc
    20701 tggttcagag cgttgtcgta aaaatcctgt gcaaataaat tagtcatata tcaagccatt
    20761 tacgcggtaa ctggcaggtc tgaaatttaa cctcctgacg atgcaggctt gcatacatcc
    20821 atagttttac tttggtatgt gactctaagt atgtagatca tcagtccggc atggaacgct
    20881 gttttgaccc gctaaaatcc ttacggaaaa gtaaggatgt ggatttgccc ctatatttcc
    20941 agtcatcggt tcgtctgaga atgacgtgca actgcgcacg cgacacccgg agacaacggc
    21001 tgactaagct tactccccat ccccgggcaa taagggcgcg tgcgctatcc acttttttgc
    21061 acgcccatat tcaacggctt ctttaaggag ttcattttcc atcgtttttt tgccgagcag
    21121 gcgctggagt tctttaatct gcttcatggc agcagcaagt tcagaggcag gaacgacctg
    21181 ttctcctgcg gccacagcag taagacttcc ctcctggtat tgcttgcgcc agagaaataa
    21241 ctggctggct gccacaccgt gttgccgggc aacaagggag accgtcattc ccggttcaaa
    21301 gctctgctga acaatagcga tcttttcctg tgtagtacgc cgtctgcgtt tctccggtcc
    21361 taagacatca atcatctgtt ctccaatgac tagtctaaaa actagtatta agactatcac
    21421 ttatttaagt gatattggtt gtctggagat tcagggggcc agtctaaatt ccgtattgct
    21481 ggttctttat cggtagaaaa atcaatatcc atagcatcgg tcgcaggcgc tgatacattg
    21541 cagagcgacc gggtattaga gtcgctattc actggttatc actgaacgct gcgtgatcag
    21601 ggttgcatta ataaaatgat atgttataac atatcaatat taacatatat tttcgcatgt
    21661 atatgaacgt aatcagaaac gtcatttgta cattaatcat actcccgata gggttacagg
    21721 cagagaccag tcattcttct atggctaaag atacaatcac cgttgtcgcg acaggaaatc
    21781 agaacacggt atttgaaacg ccgtcaatgg tcagtgtcgt cacgaatgac acgccatgga
    21841 gtcagaatgc ggtcacatca gccggcatgc tgaaaggtgt tgccggtctc agccagactg
    21901 gtgcaggacg gaccaatggg cagaccttta atctacgcgg ctatgacaaa agcggggtac
    21961 ttgttcttgt tgacggcatc cgtcaactca gtgacatggc aaaaagcagt ggcacttatc
    22021 tggatccggc actcgtcaaa cgtatcgaag ttgtccgcgg accaaactcc agtctgtacg
    22081 gcagtggcgg gctgggaggt gttgtggact tcagaactgc cgatgcagca gatttccttc
    22141 cccccggaga gacaaacggt ttaagtctgt ggggaaatat cgccagtggt gaccacagca
    22201 caggctcggg gctcacctgg ttcggtaaaa ccggaaaaac agatgcgctc ctttctgtca
    22261 ttatgcgtaa aagaggtaat atctatcaaa gtgatggtga gcgcgcacct aacaaggaaa
    22321 aacctgcagc cctgtttgcg aaaggctctg tcggtataac agacagtaac aaagcaggtg
    22381 ccagcttgcg tctctaccgg aataacacca ctgaaccggg caatcccact cagacacatg
    22441 gtgacagcgg cctgcgtgac agaaaaacag tacaaaatga cgtacagttc tggtaccagt
    22501 acgctcctgt ggataacagc ctcatcaatg taaagtcaac tttatatctc agtgatatca
    22561 ctatcaagac aaacggtcac aacaaaacgg cagaatggag aaacaacaga acctccggtg
    22621 ttaatgttgt caacaggagt catactctga tttttccggg agcccatcag ttaagttatg
    22681 gcgctgaata ttaccgtcag cagcagaagc cagaaggctc tgccacacta tatccggaag
    22741 gaaacattga ctttacatcg ttgtatttcc aggatgaaat gacaatgaaa agctacccgg
    22801 ttaacattat cgtcggttcc cgctatgacc ggtacaagag cttcaatccc cgtgccggag
    22861 aactgaaagc cgaacgcctg tccccaaggg cggcgatttc agtctcaccg acagactggc
    22921 tgatgatgta cggctccata tcctctgcat tccgtgcccc cacaatggca gaaatgtaca
    22981 gggatgatgt acatttttac cgcaagggta aacccaatta ctgggtacct aaccttaatc
    23041 tgaaaccaga aaataacatt acccgtgaga ttggcgcagg tattcaactg gacggcctgc
    23101 ttacaggcaa tgatcggctg cagttgaaag gcggatattt cggaacggat gccagaaact
    23161 atattgccac gcgcgtggat atgaaacgga tgcgttctta ttcttataat gtatcccggg
    23221 cccgtatctg gggatgggat atgcagggaa attaccagtc tgattatttt gactggatgc
    23281 tttcttataa ccgcacggaa agtatggatg ccagcagcag ggaatggctg ggctccggca
    23341 atcctgacac actcatcagt gacataagta tacctgttgg tcatggagga gtttctgccg
    23401 gatggcgtgc tgaactttcg gcagcagcca cgcatgtgaa aaaaggcgat ccccatcagg
    23461 ctggctatgc cgtacattcc ttttcactgt cttataagcc tgtaagtgtt aaaggctttg
    23521 aggcatcagt gactctggat aatgccttca acaagcttgc catgaatggc aaaggagtgc
    23581 cgctttcagg cagaactgtc agtctttata cccgttatca gtggtaacaa atcatgatga
    23641 aaaatacagg ttatatctta gctctttgtc tgacagcatc ggggcatgtc ctggcccacg
    23701 atgtctggat tacaggtaaa caggcagaga acaacattac cgcagagatt ggttatggtc
    23761 ataatttccc ctcaaagggc acaattcctg acagaaggaa tttctttgaa aatccccgga
    23821 tttataacgg gaaagagaca ataacactga agccagcgtc cacggattat gtctataaaa
    23881 ctgagtctgc aagcaaagat aatggctacg ttttgtcaac atatatgaaa ccgggatact
    23941 ggtcgagaac ctcttcagga tggaaaccgg tcagccggga gggcagaaat gatgtggctt
    24001 actgtgagtt tgtcactaaa tatgcgaaat cttttattcc tggtgaacag cagatgccag
    24061 cacaactcta tcagtctcca acggggcatg agcttgaaat cattccgtta tccgatataa
    24121 gtcgtttcag tgaagatgtg aagctgaaag tcctgtataa aacgtccccg ctcgccggag
    24181 ctatcatgga gcttgactcg gtcagttatc tgaaatcatc ccgtcatact catgcagttg
    24241 agcacaaaca tcctgttcat aaagcagaac tcacctttgt aactaatgag gatggtatcg
    24301 tcacagtacc ttctcttcat attggacagt ggctggcgaa agtccaaaat aagaaaagtt
    24361 ttcaggacaa aagcctgtgt gatgaaactg tcgatgtggc aactttaagt ttctcccgaa
    24421 attaacaata ctattccctg ccctgaaatt gtaagcgtgc agcaagaacc gtattgacgg
    24481 ggatgtgtta ttcagtcggc agtgctacgc gccaggggag caattcgccg acccggttta
    24541 tcggccagtc ggctatgacg tcaaggacat agcggaggta gctttctggc tccactccgt
    24601 tcagtttgca cgtcccgatc aggctgtaca gcagcgctcc ccgctctcct ccatgatccg
    24661 aaccgaagaa caggtagttt ttgcggccca gactgaccat ccgcaacgca ttttcagcga
    24721 tgttattgtc cgcctcagcc cagccatcat ctgcatagta cgtcagcgcc ggccactggt
    24781 tcagggcgta tgcgaacgct ttcgccagtt ctgagtgtcg cgacagggtt ttcatctttt
    24841 cacgcagcca gctttccagg gatttcagca gcggtttcgt tttcaactga cgttcggcaa
    24901 ggcgttgctc cgccgtcatt ccccttatct ctgcctcgat ggcgtacagt tcgccgatcc
    24961 gtttcagcgc ttcctccgtc agggctgacg gggtgcgaac gtgcacatcg tggattttac
    25021 ggcgggcgtg agcccaacag gcggcttccg ttatccggcc atcccggtac agctcgttga
    25081 acccggcgta tgcatcctgt caacgacgga tgaaaagtga tccacttata tctccaccaa
    25141 cggcccaata ttgatccacc gttttactca ggattagctt ctgctataac cccggccttt
    25201 cgtttctgtc tgagtcgata gctttctcct ttgatttgaa cgacatgtga gtggtgtaag
    25261 atacggtcca gcatcgctga ggtcagtgct gcatcaccgg cgaacgtttg atcccactgc
    25321 ccgaacggca gattggatgt caggatcatt gcgctctttt cgtaacgttt agcgatgacc
    25381 tggaagaaca gctttgcttc ttcctgactg aacggcagat agcctatttc atcaatgatg
    25441 agcaggcggg gggccattac tccacgctga agcgtcgttt tataacggcc ctgacgttgt
    25501 gccgtagata actgaagtaa cagatctgct gctgttgtga agcgaacttt gatacctgca
    25561 cggactgctt catagcccat cgctattgcc agatgggttt tccccacacc tgatggcccc
    25621 agtaatacga tattttcatt acgttctatg aagctgagtg agcgtaacga ctggagttgc
    25681 ttctgcggtg ctccggtggc gaatgtgaag tcatactctt cgaacgtttt caccgccggg
    25741 aaggctgcca ttcgggtata catcgcctgt ttacgttgat gacgtgccag tttttcttca
    25801 tgaagcagat gctccaggaa gtccatataa ctccattcct ggtctactgc ctgttgtgac
    25861 agcgcaggcg ctgcgctt
//
