LOCUS       NZ_CP076347             2113 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Escherichia coli strain AVS0096 plasmid pAVS0096-c, complete
            sequence.
ACCESSION   NZ_CP076347
VERSION     NZ_CP076347.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19493560
            Assembly: GCF_018798845.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Biggel,M., Hoehn,S., Schmitt,K., Frei,A., Jans,C. and Stephan,R.
  TITLE     Complete Genome Sequence of Escherichia coli Sequence Type 1193
            Isolate AVS0096, Recovered from River Water in Switzerland
  JOURNAL   Microbiol Resour Announc 10 (31), e0060721 (2021)
   PUBMED   34351235
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 125.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018798845.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 03:02:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,984
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,648
            CDSs (with protein)               :: 4,648
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 89 of 223
            Pseudo Genes (incomplete)         :: 150 of 223
            Pseudo Genes (internal stop)      :: 39 of 223
            Pseudo Genes (multiple problems)  :: 49 of 223
            Pseudo Genes (short protein)      :: 1 of 223
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076347.1.
            Bacteria and source DNA available from Institute for Food Safety
            and Hygiene, Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AVS0096"
                     /isolation_source="river water"
                     /db_xref="taxon:562"
                     /plasmid="pAVS0096-c"
                     /geo_loc_name="Switzerland: Lorze"
                     /lat_lon="47.2029 N 8.4323 E"
                     /collection_date="2020-08"
                     /collected_by="AVS"
                     /subtype="ST1193"
     gene            complement(join(2036..2113,1..888))
                     /locus_tag="KM937_RS24690"
                     /old_locus_tag="KM937_24690"
     CDS             complement(join(2036..2113,1..888))
                     /locus_tag="KM937_RS24690"
                     /old_locus_tag="KM937_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            complement(1766..2032)
                     /locus_tag="KM937_RS24695"
                     /old_locus_tag="KM937_24695"
     CDS             complement(1766..2032)
                     /locus_tag="KM937_RS24695"
                     /old_locus_tag="KM937_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          complement(264..495)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gtcatcagtg ccatccccta catcgtcggc aatgacaaga tcctcattgg tttctcgatc
       61 caactggagg acgtttttta gcgccccacc ggtcgagata aaacggcgct tgtgaagctg
      121 ccgcgtcagc tcaagaaacc actcaggatc gtttgccata tcttccggtt taacggagta
      181 tttcagcgtt tcagcaaccg cgctttgcag ttgctcggca acgttggcca caacctcacc
      241 tgtcttagtt tttactgccc gaatatcgat attcggctca tagttcaccc gcaagcaatc
      301 gcgccagagt tctacccaac gttcgtgctt aacgtagttc ttccctttaa accaagaagg
      361 ttgcaccatc agcagacagt gaaaatgcgg atgtgcgctg ccatccttac ctcgcgtcac
      421 ctccgtagcc ctgatccacc cctgaacagg tgatagctcc tttcgctttt ccattcgctt
      481 aaacgccgca ttcattgctg taaggactgt tccaagttca cctatctcgc agttcctgac
      541 agttaacgtc agaaacaacc atcgggaaga cgggtaatcc acaacgattt tcggtagagc
      601 ctgataaaaa cgggcttgcc acatgagggt tcttctccac tggcagacag ggcaatgacg
      661 gacacggcaa aattgcgcac tacgaagccg taggcgcgtt tctccggttt ccatgatggt
      721 agaccaacca aaacgtaaca aaccgctaca ggaggccata cggagtgcat agcgctcgaa
      781 ttcagcagca gtctggtaga tacccccgac agaatcggta agcgtgcgct gggtatccca
      841 aactgcatca cgggggctgt agtccgaaag gaatttatct tctgacatta agcctgctcc
      901 gatctgggca ggctgtgatt tgatgtggta tattcatcac gtacctgtgc attgcgagcc
      961 agtacatgtc accagagttt gccgctgagg tgacacattc gaaatgttgg ggagtgttga
     1021 cgcactcccc aacgccgatt aaaaacgcct caattctgct ccattttctt tgacctgtca
     1081 aaacccactc aaagcctcgt cattactggg ctaaaccact atttcatatc cgccatttat
     1141 cccttaagta taaatacaag gatcacgcgc gagaattgcg caaaaaataa ccacaatttt
     1201 aaccccccaa aacaagcacc cccgaacggc ttaccccaac cttttccaca cacccgatca
     1261 ccaaaacagg gaataaaaac aaaaccctgt cttacaggtg gatggcctca cttcgtgaaa
     1321 ccatccaccc cgcataagtt ttttaatcgg tggtgtcgtc aatcactaaa tcggaacccc
     1381 aaaggggacc cccacgattt agcatttgac gagattaact ctcggagatg gcatcatgct
     1441 tttaacggac tttaaaaaac ggtgaacgga tgcggatttt aatccgtgtc tgtgaggcgt
     1501 tgagcaacga gcaaagcgag gcggtagaaa gagagccagc ggcaggctca ggacgataat
     1561 ccggggaaaa tgtaccactc gcagagtgtt aacatttgac atcggattgt ctatcctgca
     1621 ttggtgtcga gttggcaagg ctgaggataa tgctgaggat aatgctgagg ataatgctga
     1681 ggataatgca ggatagacag agcactagag attgcatgca atctcgtacc tatcacctca
     1741 gcctgccgct ggctcttttc cagaactaag aatgcgttct taaaaatcca aacttgatac
     1801 cgtttaaatc tattttcaca aaaagagttt ttaccctctc gtttgttttc tgaatatata
     1861 aaattaattc atcctcaaaa ccttcgtgcg gatgaaaacc aaatgtgcca aaacttaatc
     1921 catcaataag caaaataagg tttttaaacc tgtcaggaat caccgcttca cttcttaaaa
     1981 aaaataaacc aatagtctta tttccattca ggtattcaat attaatattc atacgttaat
     2041 ccgatttatc cttctcaggg gcgcgtttgt aacgccgttt acctgaatcc cagacaaacg
     2101 ccgtccgctt ccc
//
