LOCUS       NZ_CP076321             4073 bp    DNA     circular CON 26-JUN-2023
DEFINITION  Escherichia coli strain Survcare045 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP076321
VERSION     NZ_CP076321.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19349416
            Assembly: GCF_018771545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Nordmann,P., Yao,Y., Falgenhauer,L., Sadek,M., Imirzalioglu,C. and
            Chakraborty,T.
  TITLE     Recent emergence of aztreonam-avibactam resistance in NDM and OXA-48
            carbapenemase-producing Escherichia coli in Germany
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Yao,Y., Falgenhauer,L., Chakraborty,T. and Imirzalioglu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2021) Institute of Medical Microbiology,
            Justus-Liebig-University Giessen, Schubertstrasse 81, Giessen 35392,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/26/2023 02:31:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,066
            CDSs (total)                      :: 4,948
            Genes (coding)                    :: 4,684
            CDSs (with protein)               :: 4,684
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 264
            CDSs (without protein)            :: 264
            Pseudo Genes (ambiguous residues) :: 0 of 264
            Pseudo Genes (frameshifted)       :: 84 of 264
            Pseudo Genes (incomplete)         :: 176 of 264
            Pseudo Genes (internal stop)      :: 56 of 264
            Pseudo Genes (multiple problems)  :: 48 of 264
            Pseudo Genes (short protein)      :: 4 of 264
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076321.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Survcare045"
                     /isolation_source="Skin swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="Germany: Hesse"
                     /lat_lon="50.04 N 8.14 E"
                     /collection_date="2018"
                     /collected_by="IMM Giessen"
     gene            complement(join(3845..4073,1..473))
                     /locus_tag="KNN08_RS25080"
                     /old_locus_tag="KNN08_25075"
     CDS             complement(join(3845..4073,1..473))
                     /locus_tag="KNN08_RS25080"
                     /old_locus_tag="KNN08_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1007..1261)
                     /gene="mobC"
                     /locus_tag="KNN08_RS25085"
                     /old_locus_tag="KNN08_25080"
     CDS             complement(1007..1261)
                     /gene="mobC"
                     /locus_tag="KNN08_RS25085"
                     /old_locus_tag="KNN08_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            1440..3587
                     /gene="mobQ"
                     /locus_tag="KNN08_RS25090"
                     /old_locus_tag="KNN08_25085"
     CDS             1440..3587
                     /gene="mobQ"
                     /locus_tag="KNN08_RS25090"
                     /old_locus_tag="KNN08_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(1263..1439)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1440..1798
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3846..4073)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.13e-115"
     Typing          1354..3584
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctgcggaact gctggagcag ctcgtaaatg cggatgctgt aggtgctgga aaggctggag
       61 acgttcttca cgactacccg cgtaaactgc cccttgagct gtgtcagata cggcatgatg
      121 gcatcggaga aggtgatatc tactttcgcc tcacctttga aatactgcgc ccggtactgc
      181 acccagcgga actcttctcg tttttcctcg ttcttgatga ttactgacct gtcccagagg
      241 cgatctatcg cttccttgag cctagcttca gcgtgctgcc gtcccatgtc aggaaaggca
      301 tcatgaaact ctgcagccgt gatggtcatg gttttttgtt catccggcga aggggaatct
      361 tctccggatt ttaggcggct gatacagacc agcaggaagc gctgttcctg caaagtgagc
      421 ttgtagcttg cctcgataag ggcgtttgat ttgtaagcta tcagatctga catttcgctt
      481 gctcgttgct attggtggcg tttttttact gtacgccact tatcatatgg gtggaagttt
      541 tttttttgca accccctttt tctgtggatt acacccccaa aagctccacc tttcaccccc
      601 aaaagctcca cctttcaccc ccaaaagctc cacctttcac ccccaaaagc tccacttatc
      661 ttgccagaac atctttaaaa tcatggtgtt acaagaccta aaaaggtttt aaaaaaaaag
      721 atctaaaaat aagatcttaa aaacttaacg gcgagcctgt ggataacttg gggtgctgaa
      781 aacaaaaaac caataaaaac aatgcgttgc aataaagcag aattgcaagt ttcgaataca
      841 ccccctccaa ccctgtggaa acccagtatt catgcgggtt cagcgcatat cacctgccga
      901 aaaaccgtgc gtatgtgctc aacctggcga ctgattcact tccgcttcct cgcccactcg
      961 ctcgctaacg ctcggtcgct ggggctgcgg cgagcggacg atacaatcac tgttctggca
     1021 gcgcctccag ctcgttaatc agcggcagta tgcgcgtcac atcagcctgt cgtgtcacct
     1081 ctgccttcag gaactggagt aaccaggcac gcaactgcac ctcctccggc tttctgacct
     1141 ttgccagcaa tgctgcaccc acaacaacct tctgcgctgt gtccaggctc cggctcttcg
     1201 ccttcaggcg ctgtaatctc gcctcagcat cagcaatctt ctgttcgagc gttctgctca
     1261 tgatgtaact ccgtgcgcga tgaaaaatcg cattttagcg cgtcactggt agtttaaaaa
     1321 ctgagctggc ataatgcacg gcaaatcacg aagtgcgcac ttatacaatc tccactccgt
     1381 ttcgattgtg tgcgccctgc ggggctgaaa gaaaactaca aaaaggcatt acggcagaaa
     1441 tggcgattta tcatctcagc atgaaaatta tctccagaag cagcggctac agcgccgtag
     1501 cttccgctgc ctatcgttcc ggttcactga tgcttgatga gcgtaccgga ctgactcacg
     1561 actacacccg caaaagcggt gtggctgagg ctgtgattct gacccccgcc accgcccctg
     1621 cctggtgtac gaaccgtgcc gaactctgga atgccgttga gaaggccgaa cgccggaaga
     1681 actcacaact tgcgcgggaa attgaactcg ccatccccca cgaactgccg caggatgccg
     1741 cacgggaaac cgttctcgcc tttgtcagag aaaactttgt cagtcagggc atgattgctg
     1801 atgtggcgtt ccatcacatg gataaaacaa atccccatgc gcacatcatg ctgaccacga
     1861 gggctatcgg acctgctggg ttcagcggaa aggtcaggga ctggaacgac cggacacatg
     1921 ccgagacgtg gcgggcatca tgggctgacc atgcaaacag ggcactggcg aacgccggtt
     1981 atcaggaaga gatagaccat cgttcatacg agcgacaggg gcttgagaaa acgcctggta
     2041 ttcacctcgg aaagtctgcc tgtgcgatgg aaacacgcgg tatcgaaaca gaacgcggcg
     2101 aacagaatcg tctgattaac aggcttaacc tggaaataca gatttcccgc accgaactca
     2161 ggaacggagg gctgacagag cctgcccgcc gtgtggctga ccttctgaat atcgggcttc
     2221 ctgacaacgc aacttcgtac acactacgtg acattatcga agcgttgccg aaggacagta
     2281 actcggcatg gaaactcacc agtaatgcca tgtcgatgat ggcagatatg caggcgacgc
     2341 gcagacgctg ggaagagctg aacaccgaac gaaaggaagc gatgagccat gctgccagcc
     2401 tgaaaaaatc atcgccgttt tcgtccggtt tttcccgtat tcccctgatg caatgggcgg
     2461 caccggaata ccgtagtgag cagggaaaaa tccagtctct gagccagcaa atggaaaaat
     2521 tacgacagca ttaccggaag gtggagaaac aggacattcc cgcgagtcag atggcatttg
     2581 aaaaacagtg gcagcagtgg ggggaatccg gccttgcagc tctcagagcc cagttgctta
     2641 aacgtgaaga ggaattgcgc cggaaggaac aggaagatac tgaacgtagt cgcgcggagc
     2701 agctcaggaa aaatgacaat gccgttatgg attcaggggt gctgagagca gtcgttacgg
     2761 ggtatggcga ggctcccatg cctggtaacg gggaaatgac ctgttacctt ctgcttcaca
     2821 accggaacgg agaatatacg ctctggggga acgagctgga gaaataccgc accagaatct
     2881 ttgagagtgt tgatttaatg cgtgacagga gtggttatat ctgtgaccga agcgaaatcg
     2941 gccaacgtcc gccactgcaa cgtgtttatt cctctgcaac ctttgagcaa ctgctgacgc
     3001 aggtttgcca gacatggccg caacataccc gcaatctccg ccagccgaag acatggccgg
     3061 agtctttctg tcttggagaa gaccgccagc cagccatgcc atcaccagcg gcaaggaagg
     3121 tggattttat gcagggacgt ctgccaccga cgctgatgcc ggtcatgagt tctgtcgacc
     3181 gggaaacccg ccagcttcag cttttgctgg tcatgggcgt ggatgactct ctgggcggtg
     3241 ttatccggct taatggtacg ctgtatcccg cgtttgccgt tccctcagcc gataacaggc
     3301 atctggtcat cagtgcgctg accgataagg ggttacggta tgcagggtat ggtgttgccg
     3361 taaatcacga tgcagacagc cacatatcac ctgcaccaga actgatgcag tttcatctga
     3421 aaacccgtga ggcaccgctt tttgctgccg tcaacacacc agaaaaacag ccggatcatc
     3481 tgttccgctc gctgggtttt aatcgtacct gggaggaatg gcagcgcgag gaagatgcac
     3541 gaacacacac caccgagagg agacatgatc gcggttggtc acagtgaatg tgaacaatat
     3601 tttgttttgt ttaacatcgt gttatgttcg tcgatgttct tcgatgttca tcgacaggga
     3661 tgaatgcaag aagcacactc acataacgca agcatagttt tgtttttccg cccccaaaaa
     3721 ttggggggtc ttttttcgct gcatcagagc gtttttgacg caatttttca cttcaatagt
     3781 accttcgcac tactaacaca aaaaaaacgc agcagggagc gttttaggtg ccttaaaatc
     3841 gattttagat cgtcatcttg atctgtttgt cttccttgaa gcgaaaatgc agggctacaa
     3901 ccgtgcgccc ctttttgatc gtctcaacag ttacaaccag atcgcttttt tcgttcagtt
     3961 cggcgatggc tggcttgatt atcatcttat tcaagtcttt gaattgttga tatttatctt
     4021 caacggcaag catggttcgg aaatcatcga tagctatcgt tctgtcaccg gtg
//
