LOCUS       NZ_CP076320            13906 bp    DNA     circular CON 26-JUN-2023
DEFINITION  Escherichia coli strain Survcare045 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP076320
VERSION     NZ_CP076320.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19349416
            Assembly: GCF_018771545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13906)
  AUTHORS   Nordmann,P., Yao,Y., Falgenhauer,L., Sadek,M., Imirzalioglu,C. and
            Chakraborty,T.
  TITLE     Recent emergence of aztreonam-avibactam resistance in NDM and OXA-48
            carbapenemase-producing Escherichia coli in Germany
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13906)
  AUTHORS   Yao,Y., Falgenhauer,L., Chakraborty,T. and Imirzalioglu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2021) Institute of Medical Microbiology,
            Justus-Liebig-University Giessen, Schubertstrasse 81, Giessen 35392,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/26/2023 02:31:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,066
            CDSs (total)                      :: 4,948
            Genes (coding)                    :: 4,684
            CDSs (with protein)               :: 4,684
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 264
            CDSs (without protein)            :: 264
            Pseudo Genes (ambiguous residues) :: 0 of 264
            Pseudo Genes (frameshifted)       :: 84 of 264
            Pseudo Genes (incomplete)         :: 176 of 264
            Pseudo Genes (internal stop)      :: 56 of 264
            Pseudo Genes (multiple problems)  :: 48 of 264
            Pseudo Genes (short protein)      :: 4 of 264
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076320.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Survcare045"
                     /isolation_source="Skin swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="Germany: Hesse"
                     /lat_lon="50.04 N 8.14 E"
                     /collection_date="2018"
                     /collected_by="IMM Giessen"
     gene            join(13472..13906,1..263)
                     /locus_tag="KNN08_RS25005"
                     /old_locus_tag="KNN08_25000"
     CDS             join(13472..13721,13721..13906,1..263)
                     /locus_tag="KNN08_RS25005"
                     /old_locus_tag="KNN08_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223368647.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(722..1705)
                     /locus_tag="KNN08_RS25010"
                     /old_locus_tag="KNN08_25005"
     CDS             complement(722..1705)
                     /locus_tag="KNN08_RS25010"
                     /old_locus_tag="KNN08_25005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_151166972.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENKGLSVARNVAIESSTGEYIWFLDSDDKISHGSLKLIDKI
                     TNEKNYGIIFFNTHVFFDTPELKKKGWHDYKRSTFGEMDALTFFEKEMTNKKLIVSACC
                     YVFRRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEALYLRRVRNDSIMTSKK
                     SMRHIIGYSTCIDELTKYKKTKGIQNKSFDRFVNGMVLSLFSIAETIKTKEAMNYVARY
                     KKHPDLSFKDRALINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(1790..2158)
                     /locus_tag="KNN08_RS25015"
                     /old_locus_tag="KNN08_25010"
     CDS             complement(1790..2158)
                     /locus_tag="KNN08_RS25015"
                     /old_locus_tag="KNN08_25010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020936656.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            complement(2189..3417)
                     /locus_tag="KNN08_RS25020"
                     /old_locus_tag="KNN08_25015"
     CDS             complement(join(2189..3102,3102..3417))
                     /locus_tag="KNN08_RS25020"
                     /old_locus_tag="KNN08_25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="WP_088895425.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACN
                     GLSDNRCLEI"
                     /name="IS3-like element IS2 family transposase"
     gene            complement(3877..4026)
                     /locus_tag="KNN08_RS25025"
                     /old_locus_tag="KNN08_25020"
     CDS             complement(3877..4026)
                     /locus_tag="KNN08_RS25025"
                     /old_locus_tag="KNN08_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_213837040.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAEVTEENLHALVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            5111..5428
                     /locus_tag="KNN08_RS25030"
                     /old_locus_tag="KNN08_25025"
     CDS             5111..5428
                     /locus_tag="KNN08_RS25030"
                     /old_locus_tag="KNN08_25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_032242326.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="lipoprotein"
     gene            6611..6745
                     /locus_tag="KNN08_RS25035"
                     /old_locus_tag="KNN08_25030"
     CDS             6611..6745
                     /locus_tag="KNN08_RS25035"
                     /old_locus_tag="KNN08_25030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072003336.1"
                     /translation="MHEPPVQSDRCVLSGNYRLESNPERHAKTPLAAAIGKQDVREMQ"
                     /name="replication initiation protein"
     gene            complement(7175..7618)
                     /locus_tag="KNN08_RS25040"
                     /old_locus_tag="KNN08_25035"
     CDS             complement(7175..7618)
                     /locus_tag="KNN08_RS25040"
                     /old_locus_tag="KNN08_25035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202752352.1"
                     /translation="MLPSETMIWQPEFTDKTLSRKPGAVQFSYLKAMYHQSKIQAELKA
                     QIHEQTTVNAICHHPESIEIIAVCSTDAYYRKRKDAAFLTTCSVLMRTLKDESVPMVLR
                     KTAWRLLNERYQRIKLNQAYRIENFLLVADFEYALEEHDELAE"
                     /name="hypothetical protein"
     gene            complement(7584..8797)
                     /locus_tag="KNN08_RS25045"
                     /old_locus_tag="KNN08_25040"
     CDS             complement(join(7584..8506,8506..8797))
                     /locus_tag="KNN08_RS25045"
                     /old_locus_tag="KNN08_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_139371347.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQSEYDSQWATICSIAPKIGCTPE
                     TLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFGEGGVRP
                     PLEKMMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEI
                     QRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAV
                     AAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIVGWRVSSSMETTF
                     VLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAES
                     INGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGN
                     DDLAA"
                     /name="IS3 family transposase"
     misc_feature    complement(8400..8516)
                     /locus_tag="KNN08_RS25045"
                     /old_locus_tag="KNN08_25040"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            9193..9429
                     /locus_tag="KNN08_RS25050"
                     /old_locus_tag="KNN08_25045"
     CDS             9193..9429
                     /locus_tag="KNN08_RS25050"
                     /old_locus_tag="KNN08_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_032242304.1"
                     /translation="MTEMERQLLSALESLQTSYEQQKQAWQDSYSSLQRMFETTSQALE
                     RNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            9574..9906
                     /locus_tag="KNN08_RS25055"
                     /old_locus_tag="KNN08_25050"
     CDS             9574..9906
                     /locus_tag="KNN08_RS25055"
                     /old_locus_tag="KNN08_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032242302.1"
                     /translation="MSHAIEFIETTMFTRQIKQIATDDEIKELQKELIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSITDAEKAELKKLTQ
                     RLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            9908..10192
                     /gene="nadS"
                     /locus_tag="KNN08_RS25060"
                     /old_locus_tag="KNN08_25055"
     CDS             9908..10192
                     /gene="nadS"
                     /locus_tag="KNN08_RS25060"
                     /old_locus_tag="KNN08_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_032242301.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="nadS"
     gene            10391..11267
                     /locus_tag="KNN08_RS25065"
                     /old_locus_tag="KNN08_25060"
                     /pseudo=""
     CDS             10391..11267
                     /locus_tag="KNN08_RS25065"
                     /old_locus_tag="KNN08_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            11412..>11676
                     /locus_tag="KNN08_RS25070"
                     /old_locus_tag="KNN08_25065"
                     /pseudo=""
     CDS             11412..>11676
                     /locus_tag="KNN08_RS25070"
                     /old_locus_tag="KNN08_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(11769..13313)
                     /locus_tag="KNN08_RS25075"
                     /old_locus_tag="KNN08_25070"
     CDS             complement(11769..13313)
                     /locus_tag="KNN08_RS25075"
                     /old_locus_tag="KNN08_25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_201360865.1"
                     /translation="MKNYDVFYGGIFKTITDYKLLKIQKDIKNNMTALVSSFKWVGIGQ
                     LVKIATQLVSLFYLTHLIPPSSYGLLAMSSVVMNLASIFNDMGTGAAVIQRQEASIGFY
                     NYIYRINIFTGLFVMLLVIITSPLIVVYFNTPELYPVLFLLAFSFPISSLTITHKAKLE
                     KEMNFKVIIAIEIFSSLTGMVLAIVLANLGFGVYSLIFQTLTTISISTICFLKVSGLSL
                     SLRIKNISHSDKSGVIGFSGYLLSFNLINYFSRNLDSILIGRFFSSAILGVYSIAYRIM
                     LFPLQSLTLVFSRVFLPHLSKNINNTNNTNKQDYLNALKLILSLSAPLMLGLSATSFSF
                     TRIFFDPKWHLLGDLLHWLAPTAIIQSALSTTGSVFMAYGKTRWLFFLGCIGAFLMGLS
                     FIIGVHFDIKTLVYLYFFANLINSLPAFILLKNILKFSYIELVNVFFRTISPAFLMFLL
                     IKVWQHFSFFKNASIDFSFNVALGIFSYIVFYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     Typing          complement(4062..4235)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          4202..4710
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          6355..6719
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.913"
                     /coverage="89.0"
                     /evalue="4.8700000000000004e-167"
ORIGIN
        1 ccacactgga gcgtcttctg agcctgctgt cggcctttga ggtcgtggta tggatgacgg
       61 atggctggcc gctgtatgaa tcacgcctga agggaaagct gcacgttatc agcaagcgat
      121 atacgcagcg cattgagcga cataatctga atctgagaca acatctggca aggctgggac
      181 ggaagtcact gtcgttctca aaatcggtgg agctgcatga caaggtcatc gggcattatc
      241 tgaacataaa acactatcag taagttggag tcattacccg gtctgcgttg tcgggaagat
      301 gcgtgatctg gggttagttt ggatatagag aattattgta cggtaagcct gttttttgaa
      361 cagacttacc tatagcaata gggtgtactc ccgcgctata cggcattttc agaggtttga
      421 ccttaatcgg ataacctaaa tgcgatactg caagagcgac aacttaaaag cgatagccac
      481 tgacgcaaaa tcaacccctt acgaaccaag attggttaaa taatgcgctt aacgtacaaa
      541 aatttccgat ctccaaactg accccgatct gatccttcaa ctcagcaaaa gttcgattta
      601 ttcaacaaag ccccggaaag cgtgcgtatc gcctgaaaac ccaacccgtc acggggatac
      661 ctatccgaaa tcttatttat tcaacaaaga aaaaccttta ttaattatcg ctttgctttt
      721 attaagggtt gcttttttta ttaataatgc cattccttag gtaattaaac accttcccgt
      781 tttttagttt taaataagtc tttttaatta atgggtaatt aattaatgct ctatctttaa
      841 atgataggtc ggggtgtttt ttatatctcg ccacatagtt cattgcttcc tttgttttta
      901 ttgtttccgc tatagagaat aacgacaaaa ccattccatt cacaaatcta tcaaagctct
      961 tgttttgtat gccttttgtt tttttatact tcgtaagctc atcgatgcat gttgaatatc
     1021 caatgatgtg cctcattgat tttttagacg tcattatgga atcatttctt accctcctaa
     1081 gatataacgc ctcatccaac gagaggcata tagttgaata attgtcaaaa atcagggttg
     1141 ctataaagtg attatcctca tgatatatat gcggaatgaa tgcattgcca tttaataatt
     1201 cccttctaaa aacatagcag catgctgaca ctattagttt tttgttcgtc atttcttttt
     1261 caaagaaagt taatgcatcc atttcaccaa atgttgaacg cttataatca tgccagcctt
     1321 tttttttcag ttctggagtg tcaaaaaaaa catgtgtatt aaagaaaata atcccataat
     1381 ttttttcatt ggttatcttg tctattaact ttagacttcc atgtgatatt ttatcatcac
     1441 tatctaaaaa ccatatatat tcaccagttg atgattcaat ggcgacattt cttgccaccg
     1501 acaggccttt attttcctga tttattactt tgattctttg gtcatcaaat gacagtatga
     1561 tatctagact gccatctgtt gaaccatcat ttacacagat tatctcatag tcgttaaaat
     1621 cctgatctaa tactgattga atacattccc ttaagtattt ttcaacgtta tatacaggta
     1681 ctattatcga aaattttttt ttcacagtaa tctccaaaca atatttagtt atactaacat
     1741 caagttgtta tttatggaat gcgatttatc aacggctttg ttgaataaat cagaatcctt
     1801 tcttcctgct aaggctatct ctgaatgact caaccactct ttgagattct tcttctgctc
     1861 gcatgaagtc attcccggac agaaaaaaac cattgtcagg cccactggct tcagagaaat
     1921 tcgtttctgc agcagacaca gaatccacat cagcaagaga cggttcatct gtactttttc
     1981 tcaatctgta aagagcctta tcaaacgcaa ccagtgagat attcaggccg taacgttcat
     2041 tcagcacagt cacaatctct tccctacgaa taccattttt cttccaggtt tctatatcgt
     2101 tcttggaatc cctgacagct gcaatggctg ttcttggacc agggttaaaa ttcgccatat
     2161 accgcccata atgtctttgg atttgcccct atatttccag acatctgtta tcacttaacc
     2221 cattacaagc ccgctgccgc agatattccc gtggcgagcg ataacccagc gcactatgcg
     2281 gatgccattc gttataatgc tcgaacgcct ctgcaaggtt ctttgctgcc gttaacccgt
     2341 ctggtttggg catgatactg atgtagtcac gctttatcgt tttcacgaag ctctctgcta
     2401 ttccgttact ctccggactc cgcaccgccg tgttcttcgg ttcaagtccc aacatccggg
     2461 cgaactggcg tgtttcatta gcccggtagc atgaaccatt atccgtcagc cactccactg
     2521 gagacgacgg aagatcgttg ccgaagcggc gttccaccgc tcccagcatg acgtcctgta
     2581 ctgtttcact gttgaagccg ccggtagtga ccgcccagtg cagtgcctca cgatcacagc
     2641 agtccagcgc gaacgtgaca cgcagtctct ctccgttatc acagcagaac tcgaacccgt
     2701 cagagcacca tcgctgattg ctttctttca cggccactct gcctgtatgt gcccgtttcg
     2761 atggcggtac agcaggtttt cgctcaagca acagcgcatt ctggcgcatg atccggtaaa
     2821 cacgtttggc attgatcgca ggcataccat caagttctgc ctgtctgcga agcagcgccc
     2881 atacccgacg ataaccatac gtgggcagct ctccgataac atggtgtata cggagaagca
     2941 catccgtatc atcagtgtga cgactgcggc ggccatccat ccagtcatcg gttcgtctga
     3001 gaatgacgtg caactgcgca cgcgacaccc ggagacaacg gctgactaag cttactcccc
     3061 atccccgggc aataagggcg cgtgcgctat ccactttttt gcccgtccat attcaacggc
     3121 ttctttgagg agttcatttt ccatcgtttt cttgccgagc aggcgctgga gttctttaat
     3181 ctgcttcatg gcggcagcaa gttcagaggc aggaacaacc tgttctccgg cggcgacagc
     3241 agtaagactt ccttcctggt attgcttacg ccagagaaat aactggctgg ctgctacacc
     3301 atgttgccgg gcaacgaggg agaccgtcat ccccggttca aagctctgct gaacaattgc
     3361 gatcttttcc tgtgtggtac gccgtctgcg tttctccggc cctaagacat caatcatctg
     3421 ttctccaatg actagtctaa aaactagtat taagactatc acttatttaa gtgatactgg
     3481 ttgtctggag attcaggggg ccagtctagt cttacatttg tctcataata gtccaaaata
     3541 agagtaaaac gcaaaaaatg tcttacaatt gtcttatttt tgtcctttcc ggttcatgga
     3601 tacatgaacc gcctgatgtg gaacgctttt tatcgatctc atgcgctgcc aaagatagat
     3661 tcattcttta aaagtcaggt tacattatcc cttaaatggt catacaccat acgcaaccgc
     3721 gattttcatc gcaattgcat gaattaaaag ataaaaactg aagtccccgt ttatttatgt
     3781 aatacgcaac gtattacaac cacctgtaat tacatgaaaa taaagaaata agatgtaata
     3841 ccggcgtatt accacgtacg cgggtaagcg aaaaagctat aacgcttccc ggccgacagg
     3901 cggcccaaaa tccaccaggg catgcaggtt ctcttcagta acctccgcca ttaaatcaaa
     3961 aaggtcatat tcgccgggaa gctgcgtatc agtcgaatcc agttgttcat catgttcaga
     4021 tggcatgggg tgatatccag tagctgagag gttgttagag ggtttcgggg cgcagccctg
     4081 aaccagtcac gtagcgctag cggagtgtat actggcttac tatgttggca ctgatgaggg
     4141 agtcagtgaa gtgcttcatg tggcaggaca aaaaaggcgg caccggtgcg tcagcagaat
     4201 atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
     4261 ctgcggcgag cggaaatggc ttacgaacgg ggcggagatg tcctggaaga tgccagggaa
     4321 atacttaaca gggaagcgat agggccgcgg caaagccgtt tttccatagg ctccgccccc
     4381 ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat
     4441 aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc
     4501 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc
     4561 agttccggga aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
     4621 gactgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag acacgacaaa
     4681 gcgccactgg cagcagccac tggtaacagg attagcagag cgagttaatg taggtggtgc
     4741 tatagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     4801 ctgcgctctg ctgaagccag ttaccaggtt aagcagttcc ccaactgact taaccttcga
     4861 aaaaccacct ccccaggtgg ttttttcgtt ttcagagcaa gagattacgc gtagaaaaaa
     4921 aggaactcaa gaagatcctt tctatgctca accaccgcat ggcccatagc cgcgctggcc
     4981 gttgccggtc agctccagaa tcctcgacca atatggaacc ttgcccctcc caaaattcaa
     5041 ctaaaaaacc ctcatttcct gccgataaaa tgggtatctc ttaactatgc ctaaataaat
     5101 aaggataatt ttgaaaaaaa tattcatcct atcggcagcg ctgatcatgg caggatgcgc
     5161 aacaaagcaa tatccacagg ccgtgaacgt caccggtcca gaatcttccg caatggattg
     5221 caaagcggta gctctggaaa tcgccaaaac gcgcagtgta caacaagaaa ttgaggaaac
     5281 cggcgaattt gacgggcgca ccgtattagg tttcctgggt gattttggga taggaaatgg
     5341 catggcaaaa ggcgaagccc gcaaaaaagc agctaaccgg ctaaatcagc tggaagccct
     5401 gaaggctgca aagtgtgcag atcaataagg tacaaaaaag ccctcggggc tttttttgta
     5461 cctgccaatc agctgaattt accataacat ccgttgcgtg caccatctca gacgccctca
     5521 gggcatttct gcgccctcct gcctatttcc ggcatctttc cctgtccata tcgtgaaaaa
     5581 acgcgcctga tgatttcttc gcgtctgcat ggctatgcac acgaacgcat ggacgtcatt
     5641 ttagcggcta aaatccggcg agcgccctga aagccaccac gaaccccacc gataacggtg
     5701 aaagggtgcg cagtttactg tgcgaccgtg aggcgttggc caggccgtca ggcatggcag
     5761 gatttattgc taagggggtc ggatttaacc tggattaatc agcacacagt gatgaacggg
     5821 attcggaagg ggcacccttg cggcgcggcg atgtatacat cgctaacgtg ccttggtcat
     5881 ttattgcaag aagggagagg tagcattttg atagcaaaaa agtagcaaaa ggatagcaac
     5941 cgaaagacca gaagactctg gagatagcaa aaaagtagca aaatggtagc aaaaagatag
     6001 caaacatgcg ggagctaacc aggagtcttt agcgggccgg cggtactcca aaaacacatg
     6061 ttgtcccgct gttttgtgaa taacccagca tagctgtggg cacaaaacat ttcctgtccg
     6121 ctgctttaac ctttgtaatc caggaacgtc aggtgacgtc actttgcggc gttagcccta
     6181 tcctgcatcc tatgaaaaaa tgcagttacg gagtgtaagg tgccggacgt gaggccgaag
     6241 atattccgct tcctcgctca ctgactcgct acgctcggtc gttcggctgc ggcgagcgga
     6301 cggagaatat tcacaaaggc atatggcgat gaagacgaga taaagcgagg gaggaaaggc
     6361 cgcggcgctg ccggttttcc ataggctccg cccccctgac aagcatcaca gaatctgacg
     6421 ctcaaatcag tggtggcgaa acccgacagg acttaaagat cccaggcgtt tccccctggt
     6481 agctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     6541 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     6601 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgtcttgt ccggtaacta
     6661 tcgtcttgag tccaatccgg aaagacacgc aaaaacgcca ctggcagcgg ccattggtaa
     6721 acaggatgtg cgagagatgc agtaagagtt cttgaagtga tggcctgagt gcggctacac
     6781 tgaaaggaca gatttggtga catgtctcgc acaagactgt taccgcggtt agaaaatctg
     6841 ccaggattag ctaaccttcg aaaaaccacc tgccagggtg gttttttcgt ttacagagca
     6901 agagattacg acgcaacaga aaggatctca agaagttcct tgctgtattg cgggtccgct
     6961 tggaaaaagg aaattatcct acgttaagcg ttttaagcta acgggtgatt tcgttccatt
     7021 gagcgcgaac ccctgaccgt aagggatgta cgaaatgatc cggctgtccg ttaatggttt
     7081 tgcttacttt cggacatgtt gtctacatgc tgtacgtaat cggacgttta agtcacatgc
     7141 acgttaactg tcggtattca tcgcctcact agtactactc tgctagttca tcatgttctt
     7201 cgagagcata ttcaaaatcg gcaacaagca agaaattttc tatcctataa gcctgattta
     7261 acttaattcg ttggtatctt tcgttcaata atcgccatgc tgttttccgt agtaccatcg
     7321 gtacggattc atcttttaaa gtacgcatga gcacagaaca ggttgtcaga aaagcagcgt
     7381 ccttacgttt tcggtaatac gcatcagtcg aacaaaccgc aatgatctcg attgattcag
     7441 ggtgatgaca gatagcatta accgtagttt gttcatgtat ctgtgccttg agttcagcct
     7501 gaatttttga ttgatgatac atagccttga gatagctaaa ctgaaccgcc ccgggtttcc
     7561 tggagagtgt tttatctgtg aactcaggct gccagatcat cgtttccgat ggaagcataa
     7621 taagcttttt ctgcttctgc cggaggagta tggcccagcc tttccagcaa tcgtcgattg
     7681 ttataccagt ccacccacgt gagtgtggcc agttccactt ctgcacggtt tttccagctc
     7741 ttacggtgta ttacctccgc tttgtaaaga ccattgatgc tctccgccat cgcgttgtca
     7801 tacgagtcgc ctgtacttcc tgttgatgcc agtaatccgg cttccttaag ccgctgtgtg
     7861 taggccagcg atacatactg agaaccttta tcactgtgat ggaccgtgcc ggacggtcga
     7921 cgggcccata acgcctgctc cagtgcatcc agcacgaatg tcgtttccat ggacgatgag
     7981 acccgccacc ccacgatgta tccggcaaac acatcaatga tgaacgccac atagacgaag
     8041 ccccgccatg tgctgacgta agtaaaatca gccacccaca gctggtcagg tcgttctgcc
     8101 acgaactgac ggtttacgcg gtcgcctgcg gcaacggctt tccggctgat ggtcgtacgg
     8161 acctttttac cccggagaac accggcaagt cccataaccg ccatgagacg tgccacagtg
     8221 catctggcca ctctgatacc ttcccgtaac aactgacgcc agactttacg cacaccgtat
     8281 accttgtgat tttcatcgta tacgcgctgt atctctttct tcagccagtc atcgcgctgc
     8341 gcacgggcac tgcgtttatc cggatgatgt cgctgttgct gacagtggta atacgttgac
     8401 ggggcaatat gcagttcgct gcatagcggt ccgaccccgt actgctcacg cagcttatcc
     8461 agcagtggca tcattttttc cagaggcggt cgaactccgc cttcgcaaaa taagcggaag
     8521 cctggcgaag gatatcgtta ctgcggcgca gttcacgatt ttcacgttcc agctctttca
     8581 gacgctgacg ttcagcggtg gtgagccctc catcaccgcc cccggtatcc cgctcatgct
     8641 ggcgaaccca gacacgcaga gtctccggcg tacagccaat ctttggagca atggaacaaa
     8701 ttgtcgccca ttgtgagtca tattcgctct gactttccag aaccatacgg actgcccgtt
     8761 gacggacttc gggggaaaaa cgagtatttt tagtcatcct gtttacctct ttctcaggaa
     8821 gtttagtctc caggattccc ggggcggttc attccccatc aaacagtaag tcgtgtcaat
     8881 tcctagagtt aacacatttc tgatcatgac tcacacgtta cgttcagtcg gtagccatct
     8941 gtgcaaaaaa tgcgcttagc gtaggataat ttcctttttc caagcggacc ccagagcggc
     9001 gcaggggtag cgatttacaa ggacgacaag cagaagaaca cctatctggt ggtactgccg
     9061 aagaaagcgc atggcaccga gacatacaca tcgacagccg gaaacaccgt gatgaagtac
     9121 accgcgaaat aaccccgtca gacaccacag aaacgccaca gcacgttttt atcactgagg
     9181 acaggaaaaa ccatgaccga gatggaaagg cagcttctga gcgccttaga aagcttacaa
     9241 acaagctacg aacagcagaa gcaggcgtgg caggacagtt acagcagctt gcaacgcatg
     9301 ttcgagacta cctcacaggc actggagcgc aacgacaggg tatgtctgaa cttgagcaat
     9361 caggtgaacg ccttgcacga acaggtcgaa cgcttgagca gcgatctgag ccgattaaca
     9421 cgctcgtaaa gcagcaggaa cggcagttac agcaggagaa gcaagcaacc cgctatcgag
     9481 gtcagtccat gtaacgtctg atggcacaga acgcggcttt tcagaacacg cctcttggtg
     9541 tacattgtac atcaaaatat acaggagaca gccgtgagtc acgcgattga gtttatcgag
     9601 acaacgatgt ttacccgtca gataaagcaa atcgccacgg atgatgagat caaagagctt
     9661 cagaaagagc ttatcgagtc accggacaaa ggtgacctca tccagaaaac gggcggtctg
     9721 cgaaagatca ggatggcgac ggggaatcag ggaaagagcg gcagtgcgcg ggtgatttac
     9781 ttccttgcca ccgcagaggt gatttatctg gtgatggcct accctaaaag caccaaagac
     9841 agcatcacag acgcagagaa agcagaactg aagaagctga cccaacgact gaaagacgag
     9901 gtgtaagatg agtttttttg acgagctgaa agcctctctg gaagaggccg ttgaaatcaa
     9961 aaacggaacc aaagcccccg cccgtgtgac ccgctacgag ctggcagacg tcagggctat
    10021 cagagaaaac ctgaatatta ctcagagcga aatggcgaaa gtgctgggaa ccagcctcga
    10081 taccatcaaa agctgggaaa ccggacgccg taacccaacc ggactggcag caaaagtgct
    10141 ggccaccatt caggctaacc caaagttttt tgatgaactg gcggcgcatt aagctgatgt
    10201 gacagaagaa attcgacagg cgaggccgtt cgcgtgtaac tttaacggca acagaaaaaa
    10261 caaagccccg ataatcatga tttcaacttg gcggaagaga catgataaat cagggctagg
    10321 tataaaacct aagaaggata ttagcacatg aaccgtgcca gacaacagcc cgccataagt
    10381 tcagggcaca ttgcgtaacg cagcactcga acttttcaac gaccgcttgc cgcataaacc
    10441 gtacttctcg gatgatttac actttggtgt ccgcatcgca gggaaagagc gcgctattct
    10501 ggctaaatac atccagttta atcagcctca cgccatgttc tggctgggat ttgacgttga
    10561 ccgcatcggc gcagccattg actggagcga ccgcaacgcc cccgcgccga cactgaccat
    10621 caccaacccc gaaaacggac acgcgcacct gctgtacgca ctgaaaacct cgatacgcac
    10681 cgcgccagac ggaaaaatga aaccgttacg ttacgccgct gctgtagaga gcgcgctacg
    10741 taaaaaattg ggcgctgaca cggggtattc ggggctaatt tgcaagaatc cgaaccacag
    10801 tcactggaaa attgcccgta tggcagcctg aactctacac gctggactgg ttagccgaat
    10861 cactcgacct gaacgctgcg aacgataaaa aaatcgtcgc cgattatggc ttaggtcgta
    10921 actgcatact gtttgataaa accagaaagt gggcatatcg cgctatccgt cagggctggc
    10981 ctgagtacga acagtggcta caagcctgcc atgaacgcgc ctgcgcttac aatctgcaat
    11041 ttgcagtgcc tttgaatgag aatgaagtaa cgggaatagc gaaaagtatc tgcaagtgga
    11101 catttttgaa atttaataag caaaattttt cacttataca agccaaaaga ggaaaagttg
    11161 gtggtgaaaa atcatcagga ggaggtaggc tgtctctagg ggagccttgg gttgctttag
    11221 gtattagtcg cgctacattt tttagaaaaa ggaaaattta taattaagat tcagtatttt
    11281 acatactgaa tcttaattat tgaatcacca ttacttaagt gttgatagtg ttttttttcc
    11341 agcagtaaca actgtttgtg atgatgccaa cttgctggtt tagtgtctaa tggtgttttt
    11401 gaggtgctcc cgtggcttcc atttccatca gttatccctc ctgttcagct actgaaggcg
    11461 tggtgcgtaa cggtaaaagc actaccggac atcaacgcta tctctgctct cactgtcgta
    11521 aaacatagca agcaactaca gttcacttac accgcctctg agcccggtac acaccagaaa
    11581 atcattgata tggccatgaa tggcgttgga tgccgggcca catcccgcat tatgggcact
    11641 gtcctcaaca cgattttacg acacttaaaa aaattaggcc gaagtcggta tcgggcatta
    11701 tctgaacata aaacacaagc agtaagttgg agtcattacc aattcattta atgaatttaa
    11761 taaatgaatc actttaactt ggtcatgcat cttaacttat gaattactcc tgtcctaaca
    11821 acctcattga aattaaacaa ggtataaaaa acaatataac taaaaatacc caatgcgaca
    11881 ttaaaagaaa aatcaatgct ggcatttttg aaaaaggaaa aatgctgcca tactttaata
    11941 agtaagaaca tcaaaaaagc tggggagata gttctaaaaa acacgtttac caattcaatg
    12001 taactaaact tcaagatatt cttaagtaaa ataaatgccg ggagtgaatt aattaagttt
    12061 gcaaaaaaat aaaggtagac caatgttttt atatcgaaat gaacacctat aatgaaagac
    12121 aaccccatca aaaaagcgcc aatgcaacct aagaaaaaca accaccttgt ttttccgtag
    12181 gccataaaaa cagaacctgt tgtactcaag gctgattgga taatcgctgt aggtgccaac
    12241 caatgcaata agtctccaag taggtgccac ttaggatcaa agaaaatcct cgtaaaagaa
    12301 aaagatgtag cagataaacc tagcatcagt ggcgcgctta atgatagtat aagttttaat
    12361 gcatttaagt aatcttgttt attagtgtta ttagtgttat ttatgttttt gctaaggtgt
    12421 ggcaagaaaa ctctagaaaa aacaagcgtc aggctctgca acggaaataa cataatgcgg
    12481 taggcaatcg aatagacccc aagtattgcg gatgagaaaa aacggcctat taaaatagaa
    12541 tcaagattcc tggaaaaata atttattaaa ttaaaagaca ataaataacc actaaaccct
    12601 ataactcctg acttgtcact atggctgata tttttaattc tcaatgataa actcaacccg
    12661 gaaaccttca aaaaacatat tgtggatatt gatattgttg tcagtgtttg aaaaatcaaa
    12721 ctataaacac caaagcctaa attagctaaa actattgcca gcaccattcc tgtaaggcta
    12781 gagaaaattt caattgcaat gatgacttta aagttcattt ctttttctaa ttttgctttg
    12841 tgagttattg ttaagctgga aattggaaag ctaaaagcca atagaaatag aaccgggtat
    12901 agctcgggtg tgttaaagta cacgacaatt aatggggatg ttattattac aagcagcata
    12961 acaaacaacc cagtaaaaat gtttatcctg tatatgtaat tataaaaacc gatcgacgcc
    13021 tcttgtcgct gaattactgc cgccccagtt cccatgtcat tgaatatgct tgctaaattc
    13081 ataaccacag atgacattgc taatagtcca tagctcgatg gggggattaa gtgcgtcaag
    13141 tagaataacg aaaccaattg tgttgctatt tttacaagtt gtccaatgcc aacccattta
    13201 aaactactga ctagtgcagt catattgttt ttaatatcct tttgaatttt caataattta
    13261 taatccgtga tggttttaaa gataccacca taaaagacat cataattctt caagctatca
    13321 tctacgttgt agattccgat ttagctaaac tttaggactt tataagttcg tatcccgtat
    13381 gattataagg cattatgatc atctccacca atagtgggta atgctgccaa cttactgatt
    13441 tagtgtatga tggtgatttt aaggtgcttg cgtggcttcc atttccatca gatgtccttc
    13501 ctgctccgct actgaaggcg tggtgcgtaa cggcaaaagc actgccggac atcagcgcta
    13561 tctctgctct cattgccgta aaacatggca actacagttc acttacaccg cctctcagcc
    13621 cggtacgcac cagaaaatca ttgatatggc catgaatggc gtcggatgtc gcgccagtgc
    13681 acgcattatg ggcgttggcc tcaacacggt tttacgtcac ttaaaaaact caggccgcag
    13741 tcggtaacct cgcgcataca accgggaagt gatgtgattg tctgcgctga aatggacgaa
    13801 cattggggct acgtcggtgc taaatcacgt cagcgctggc tgttttacgc gtatgacagg
    13861 atacggagga cggttgtggc gcacgtcttc ggtgaacgca ctctgg
//
