LOCUS       NZ_CP076316             2101 bp    DNA     circular CON 26-JUN-2023
DEFINITION  Escherichia coli strain Survcare045 plasmid unnamed8, complete
            sequence.
ACCESSION   NZ_CP076316
VERSION     NZ_CP076316.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19349416
            Assembly: GCF_018771545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Nordmann,P., Yao,Y., Falgenhauer,L., Sadek,M., Imirzalioglu,C. and
            Chakraborty,T.
  TITLE     Recent emergence of aztreonam-avibactam resistance in NDM and OXA-48
            carbapenemase-producing Escherichia coli in Germany
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Yao,Y., Falgenhauer,L., Chakraborty,T. and Imirzalioglu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2021) Institute of Medical Microbiology,
            Justus-Liebig-University Giessen, Schubertstrasse 81, Giessen 35392,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/26/2023 02:31:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,066
            CDSs (total)                      :: 4,948
            Genes (coding)                    :: 4,684
            CDSs (with protein)               :: 4,684
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 264
            CDSs (without protein)            :: 264
            Pseudo Genes (ambiguous residues) :: 0 of 264
            Pseudo Genes (frameshifted)       :: 84 of 264
            Pseudo Genes (incomplete)         :: 176 of 264
            Pseudo Genes (internal stop)      :: 56 of 264
            Pseudo Genes (multiple problems)  :: 48 of 264
            Pseudo Genes (short protein)      :: 4 of 264
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076316.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Survcare045"
                     /isolation_source="Skin swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="Germany: Hesse"
                     /lat_lon="50.04 N 8.14 E"
                     /collection_date="2018"
                     /collected_by="IMM Giessen"
     gene            complement(join(1959..2101,1..124))
                     /locus_tag="KNN08_RS01615"
                     /old_locus_tag="KNN08_01615"
     CDS             complement(join(1959..2101,1..124))
                     /locus_tag="KNN08_RS01615"
                     /old_locus_tag="KNN08_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(128..1093)
                     /locus_tag="KNN08_RS01620"
                     /old_locus_tag="KNN08_01620"
     CDS             complement(128..1093)
                     /locus_tag="KNN08_RS01620"
                     /old_locus_tag="KNN08_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(469..700)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 caaaacttaa tccatcaata agcaaaataa ggtttttaaa cctgtcagga atcaccgctt
       61 cacttcttaa aaaaaataaa ccaatagtct tatttccatt caggtattca atattaatat
      121 tcatacgtta atccgattta tccttctcag gggcgcgttt gtaacgccgt ttacctgaat
      181 cccagacaaa cgccgtccgc ttcccgtcat cagtgccatc ccctacatcg tcggcaatga
      241 caagatcctc attggtttct cgatccaact ggaggacgtt ttttagcgcc ccaccggtcg
      301 agataaaacg gcgcttgtga agctgccgcg tcagctcaag aaaccactca ggatcgtttg
      361 ccatatcttc cggtttaacg gagtatttca gcgtttcagc aaccgcgctt tgcagttgct
      421 cggcaacgtt ggccacaacc tcacctgtct tagtttttac tgcccgaata tcgatattcg
      481 gctcatagtt cacccgcaag caatcgcgcc agagttctac ccaacgttcg tgcttaacgt
      541 agttcttccc tttaaaccaa gaaggttgca ccatcagcag acagtgaaaa tgcggatgtg
      601 cgctgccatc cttacctcgc gtcacctccg tagccctgat ccacccctga acaggtgata
      661 gctcctttcg cttttccatt cgcttaaacg ccgcattcat tgctgtaagg actgttccaa
      721 gttcacctat ctcgcagttc ctgacagtta acgtcagaaa caaccatcgg gaagacgggt
      781 aatccacaac gattttcggt agagcctgat aaaaacgggc ttgccacatg agggttcttc
      841 tccactggca gacagggcaa tgacggacac ggcaaaattg cgcactacga agccgtaggc
      901 gcgtttctcc ggtttccatg atggtagacc aaccaaaacg taacaaaccg ctacaggagg
      961 ccatacggag tgcatagcgc tcgaattcag cagcagtctg gtagataccc ccgacagaat
     1021 cggtaagcgt gcgctgggta tcccaaactg catcacgggg gctgtagtcc gaaaggaatt
     1081 tatcttctga cattaagcct gctccgatct gggcaggctg tgatttgatg tggtatattc
     1141 atcacgtacc tgtgcattgc gagccagtac atgtcaccag agtttgccgc tgaggtgaca
     1201 cattcgaaat gttggggagt gttgacgcac tccccaacgc cgattaaaaa cgcctcaatt
     1261 ctgctccatt ttctttgacc tgtcaaaacc cactcaaagc ctcgtcatta ctgggctaaa
     1321 ccactatttc atatccgcca tttatccctt aagtataaat acaaggatca cgcgcgagaa
     1381 ttgcgcaaaa aataaccaca attttaaccc cccaaaacaa gcacccccga acggcttacc
     1441 ccaacctttt ccacacaccc gatcaccaaa acagggaata aaaacaaaac cctgtcttac
     1501 aggtggatgg cctcacttcg tgaaaccatc caccccgcat aagtttttta atcggtggtg
     1561 tcgtcaatca ctaaatcgga accccaaagg ggacccccac gatttagcat ttgacgagat
     1621 taactctcgg agatggcatc atgcttttaa cggactttaa aaaacggtga acggatgcgg
     1681 attttaatcc gtgtctgtga ggcgttgagc aacgagcaaa gcgaggcggt agaaagagag
     1741 ccagcggcag gctcaggacg ataatccggg gaaaatatac cactcgcaga gtgttaacat
     1801 ttgacatcgg attgtctatc ctgcattggt gtcgagttgg caaggctgag gataatgctg
     1861 aggataatgc tgaggataat gcaggataga cagagcacta gagattgcat gcaatctcgt
     1921 acctatcacc tcagcctgcc gctggctctt ttccagaact aagaatgcgt tcttaaaaat
     1981 ccaaacttga taccgtttaa atctattttc acaaaaagag tttttaccct ctcgtttgtt
     2041 ttctgaatat ataaaattaa ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg
     2101 c
//
