LOCUS       NZ_CP076313             3372 bp    DNA     circular CON 26-JUN-2023
DEFINITION  Escherichia coli strain Survcare045 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP076313
VERSION     NZ_CP076313.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19349416
            Assembly: GCF_018771545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Nordmann,P., Yao,Y., Falgenhauer,L., Sadek,M., Imirzalioglu,C. and
            Chakraborty,T.
  TITLE     Recent emergence of aztreonam-avibactam resistance in NDM and OXA-48
            carbapenemase-producing Escherichia coli in Germany
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Yao,Y., Falgenhauer,L., Chakraborty,T. and Imirzalioglu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2021) Institute of Medical Microbiology,
            Justus-Liebig-University Giessen, Schubertstrasse 81, Giessen 35392,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/26/2023 02:31:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,066
            CDSs (total)                      :: 4,948
            Genes (coding)                    :: 4,684
            CDSs (with protein)               :: 4,684
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 264
            CDSs (without protein)            :: 264
            Pseudo Genes (ambiguous residues) :: 0 of 264
            Pseudo Genes (frameshifted)       :: 84 of 264
            Pseudo Genes (incomplete)         :: 176 of 264
            Pseudo Genes (internal stop)      :: 56 of 264
            Pseudo Genes (multiple problems)  :: 48 of 264
            Pseudo Genes (short protein)      :: 4 of 264
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076313.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Survcare045"
                     /isolation_source="Skin swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="Germany: Hesse"
                     /lat_lon="50.04 N 8.14 E"
                     /collection_date="2018"
                     /collected_by="IMM Giessen"
     gene            complement(join(3259..3372,1..405))
                     /locus_tag="KNN08_RS25105"
     CDS             complement(join(3259..3372,1..405))
                     /locus_tag="KNN08_RS25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_252237641.1"
                     /translation="MKERAASTENMLNSEFRKLEESVDKALSLNRQKIRDAISEHTTSV
                     KQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTALSVTGGIFLGCWGL
                     IQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVLPDGMKGENTWTVGD
                     KNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<293..1135)
                     /locus_tag="KNN08_RS00035"
                     /old_locus_tag="KNN08_00035"
                     /pseudo=""
     CDS             complement(<293..1135)
                     /locus_tag="KNN08_RS00035"
                     /old_locus_tag="KNN08_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1125..1448)
                     /locus_tag="KNN08_RS00040"
                     /old_locus_tag="KNN08_00040"
     CDS             complement(1125..1448)
                     /locus_tag="KNN08_RS00040"
                     /old_locus_tag="KNN08_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001677263.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1511..1702
                     /locus_tag="KNN08_RS00045"
                     /old_locus_tag="KNN08_00045"
     CDS             1511..1702
                     /locus_tag="KNN08_RS00045"
                     /old_locus_tag="KNN08_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165986.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3019..3252)
                     /locus_tag="KNN08_RS00050"
                     /old_locus_tag="KNN08_00050"
     CDS             complement(3019..3252)
                     /locus_tag="KNN08_RS00050"
                     /old_locus_tag="KNN08_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1784..1864)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.805"
                     /coverage="93.0"
                     /evalue="8.68e-18"
     Typing          complement(3..1135)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.735"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2937..3370)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="82.667"
                     /coverage="99.0"
                     /evalue="1.83e-52"
ORIGIN
        1 cccccaggtt tttgcctcga gtttcgccag cgtgttttcc tgctctctga tggtcaagat
       61 atttttcgca atcctgcttt cctgccactg aatcagcccc cagcacccca ggaaaatgcc
      121 gcccgtgacg ctgagcgcgg tcagcgccgg aaacagtacc cgccctttga tcacctgcca
      181 gagcaggttt ttctgctgcc gggacagttc ggcctctgtg gtgctgagct gcgtgctcac
      241 ggtggtgctc agcgtatcca gttgctgttt cacggatgtt gtatgttcgc tgatagcgtc
      301 tctgattttc tgcctgttta agctcagtgc cttgtctaca gactcttcta gcttcctgaa
      361 ctcgctgttc agcatgttct cggtagaagc ggctcgctct ttcagtttct tctcgaagtc
      421 tgttcccatt tgtaaaagat tgctcataca gcgccccctt tagcctgaga ttccgccccc
      481 cctccgggtc ggcaatactg atactgcttc tggttgtcct caccacctca aaacctgccg
      541 ctgtgagcgc ctcagtgaca tcctgacgcg tttttattgc tccggcacgg taaagcgcct
      601 caagaccccg tgtaatcgct tctgcggcct cctgtttcgc ttttggcaga ttattcggtg
      661 tgacaagcgt ccgtctgttc tccggtgcgt tcgggtcacg cagcccgtaa tgatgattaa
      721 ccagcgtctg ccaggcattt atcctcggcc tgtccgcacg gtcgtagtac ggctggaggc
      781 gcttaccgct ctgtagttcc atgttcggaa tgacaaaatt cagttcgagc cgccccttat
      841 cctggtgttc cacccagaga atgctgtact ggtctttttc gagaccgggc ataagaacac
      901 gctcaaagct cgtcatcact ctttcgcgtt cacccggcgg taactccttc tccgcgaacg
      961 acagaacgcc tgacgtgtat ttcttcgaaa atggcgtggc atcaatgagt tcccggattt
     1021 cttccgggtt accccggagc accgttgcgc cttcgcggtt acgctcccgc cccagcaggt
     1081 aatcgaccgg gccacttcca ccgcctttcc ccctggcatg aaatttaact atcatcccgt
     1141 cccccctgtt ccctgacagc ctgccgcaac tggcgcaatt caccctcgat ggccatcagt
     1201 gcggccacca catgaacccg gtcaatcgaa gaccagtggc cgctgttcac cttccgggct
     1261 gtctggttca gattgttccc gatggcagcc agatgccgca gcaatggcgg cgatagtgtc
     1321 ggcagttttc ctgtgcgcgc aaccggctcc cccagacaga cccgccgcat ccattctgcc
     1381 agtcgtttac cctcgcagcg ttccagtaac cgggcgtgct cctcatcgct aacccgtatc
     1441 gtgagcatct tctcgcgttt catcggtatc attcccccta aaaaatcccc tattaaggag
     1501 ccttaagacc atgaccaaac aggaaaaaac agcccttaac atggcccgct ttatcagaag
     1561 ccagacgctg accctgctgg aaaaactcaa cgagctggac gcggacgaac aggcagacat
     1621 ctgcgaatca cttcacgacc acgccgacga actctaccgc agttgcctcg cacgcttcgg
     1681 cgatgacggt gaaagcaact gaaacatgca gctcccggag acgttcacag cttgcctgtg
     1741 agcggatgcc gggagcagac aagcccgcca aggcgcgtca gtgcgggtgt cggggcgcag
     1801 ccctgaccca gttcacagag cgatagcgat gtgtatactg gcttatctca ttttgcatcg
     1861 gtgcagagtg gatgggaagt gcagcatgtc aggagtaaaa agccgcacag atgcgtaaaa
     1921 acagcgcgcc cgataaacaa cacacacgcg gacactggca caggcgcgta agtgcacaat
     1981 gcttgcctct gtgactcttc cgcttcctcg cccactcgct cactacgttc ggtcgctggg
     2041 gctgcggcga gcggtgggat cactctcgtt gtgatgcaga tccgtttttt tttttgcact
     2101 ggagttatca ggagtagcgg tgcacatggg atcgtgcgat atgctggatg gtggaaggtc
     2161 ggagggaacg gcaacaactc acccctccca gtgaagggac gggtagtttg ccgctctcga
     2221 tatgccggac ggagccaagg ccatcgatca cggggcgcgt cagtggaaga acccgcgcct
     2281 gcgtgacgta tgcgtcttcg ccgcaccatc tcacccgtca cgccaccaca taccgggaac
     2341 tgccgccaag gtctaactca accaatctag ccgcgctaca tacggttttt gtggcaactc
     2401 atcaggtgtc cgctgtcgat gaaactctct gcgccggtag cggaactctc acgcgtgcgg
     2461 tcgccgtcgt cacagctccc ccaaccgcaa acgattctcg caaatacgca agtatctgca
     2521 aaaacaggtt tttttcgggg ttcctgggag actgtgctgt tgaaaaattc ttgaagaata
     2581 cagaaggctc atgcaaactg aatgaactgg tttctgttgt ggatctcaac agtttccaca
     2641 ggaacattga ggaggtcaga gacctcaatg aacccgaaac agctcgcctt cctggcgagc
     2701 tgttttcgtt ctacgcccgc cgacgaaaga tcgcaagtgc aataacgaga tttttcacgt
     2761 ctttacgctc aaaaagtaaa aaaaccatgt tttcccctgg ctgaatcccc aaaaacgcgt
     2821 gaaatgcgtt ttcaggatgt tgcatagcta tgcacaaata cggcacacga tttgtttcgc
     2881 tcgccccagc cgtcgaacgg gtcaaaatgg caagatttcg tcacttcccc ggtcacatct
     2941 ccattgatgg cccactccga acccgtgccg cgcgttcctg tacctcatgt ctgtaccggg
     3001 cacgctcctg ttcatccctt agctgttgtt cgcgataaat aaccgactca agcgatccac
     3061 ttgcccggct aatctctgca cctgctgact caatatcacg acgcgctcgt tcagtatcgc
     3121 gttgttccgt tgcgtaacct cgtgcatctt ctgcaattcc gcgaaggcgc tctcccactc
     3181 gttcagccgc cgcatatagt cctgttgcaa atgctctaag gcgttcagca aatgcccttc
     3241 cagttccgtc atactcgttt actccctgac cagcctcacg gcgttcttgt cgcccaccgt
     3301 ccaggtgttt tcccctttca tcccgtccgg caacaccaga aatttcccgt tctcgccatt
     3361 gacgaacgtc ac
//
