LOCUS       NZ_CP076312             4065 bp    DNA     circular CON 26-JUN-2023
DEFINITION  Escherichia coli strain Survcare045 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP076312
VERSION     NZ_CP076312.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19349416
            Assembly: GCF_018771545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Nordmann,P., Yao,Y., Falgenhauer,L., Sadek,M., Imirzalioglu,C. and
            Chakraborty,T.
  TITLE     Recent emergence of aztreonam-avibactam resistance in NDM and OXA-48
            carbapenemase-producing Escherichia coli in Germany
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Yao,Y., Falgenhauer,L., Chakraborty,T. and Imirzalioglu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2021) Institute of Medical Microbiology,
            Justus-Liebig-University Giessen, Schubertstrasse 81, Giessen 35392,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/26/2023 02:31:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,066
            CDSs (total)                      :: 4,948
            Genes (coding)                    :: 4,684
            CDSs (with protein)               :: 4,684
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 264
            CDSs (without protein)            :: 264
            Pseudo Genes (ambiguous residues) :: 0 of 264
            Pseudo Genes (frameshifted)       :: 84 of 264
            Pseudo Genes (incomplete)         :: 176 of 264
            Pseudo Genes (internal stop)      :: 56 of 264
            Pseudo Genes (multiple problems)  :: 48 of 264
            Pseudo Genes (short protein)      :: 4 of 264
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076312.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Survcare045"
                     /isolation_source="Skin swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="Germany: Hesse"
                     /lat_lon="50.04 N 8.14 E"
                     /collection_date="2018"
                     /collected_by="IMM Giessen"
     gene            221..334
                     /locus_tag="KNN08_RS25095"
     CDS             221..334
                     /locus_tag="KNN08_RS25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001427889.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
                     /name="hypothetical protein"
     gene            696..1088
                     /locus_tag="KNN08_RS00005"
                     /old_locus_tag="KNN08_00005"
     CDS             696..1088
                     /locus_tag="KNN08_RS00005"
                     /old_locus_tag="KNN08_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001218243.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRALQKLQI
                     QEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGNNA
                     ETQSTHYQPYPYDKVSIWGPFPHNLT"
                     /name="hypothetical protein"
     gene            complement(1558..1791)
                     /locus_tag="KNN08_RS00010"
                     /old_locus_tag="KNN08_00010"
     CDS             complement(1558..1791)
                     /locus_tag="KNN08_RS00010"
                     /old_locus_tag="KNN08_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137430.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLVEQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1798..2358)
                     /locus_tag="KNN08_RS00015"
                     /old_locus_tag="KNN08_00015"
     CDS             complement(1798..2358)
                     /locus_tag="KNN08_RS00015"
                     /old_locus_tag="KNN08_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067489.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENILNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2390..2827)
                     /locus_tag="KNN08_RS25100"
                     /pseudo=""
     CDS             complement(<2390..2827)
                     /locus_tag="KNN08_RS25100"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3036..3359)
                     /locus_tag="KNN08_RS00025"
                     /old_locus_tag="KNN08_00025"
     CDS             complement(3036..3359)
                     /locus_tag="KNN08_RS00025"
                     /old_locus_tag="KNN08_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3427..3618
                     /locus_tag="KNN08_RS00030"
                     /old_locus_tag="KNN08_00030"
     CDS             3427..3618
                     /locus_tag="KNN08_RS00030"
                     /old_locus_tag="KNN08_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3704..3791)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          2..330
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.879"
                     /coverage="100.0"
                     /evalue="7.71e-160"
     Typing          3802..4065
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.179"
                     /coverage="100.0"
                     /evalue="1.99e-87"
     Typing          complement(1452..3046)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.872"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3419..3621)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.235"
                     /coverage="100.0"
                     /evalue="5.02e-12"
ORIGIN
        1 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggacttaaa gataccaggc
       61 gtttccccct ggtggctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      121 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgttggta
      181 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      241 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      301 cttaacgcca ctggcagcag ccattggtaa ctagacaagg tggattgaga tattaagagt
      361 tcttgaagtg gtggcctaac tgcggctaca ctagaaggac agtatttggt atctgtgctc
      421 caccaagcca gttaccttcg gaagaaagag ttggtagctc tagattccgg caaacaaacc
      481 accgttggta gcggtggttt ttttgtttgc aggcagcaga ttacgcgccg caaaaaagga
      541 tctcaagaag atcttgaatc actctatgaa aattttgttt tatagccgta tttgttgatg
      601 ctataagcca gagaccttct atgagaatag acatatcaca tcaaacacgg cataccccgc
      661 caaacatgct tcctcgggaa caaaattgtg tagcaatggc attatctgcg tgctttagac
      721 aacaactaaa ccctgtggta aacagcttat taaaagagag aataatacac tcccctaaag
      781 aattggaaca tgacaacgca gtaataagag ccctgcaaaa actacaaatt caagaagttt
      841 gtaacagcac cctttgggag acagccaaac aacaactact tcaaaaatcg gacggaagat
      901 attttgccat caattctaaa cacttagcct ttccaggccc aggagaatct cacgccttct
      961 gttgcatcaa atacaaaaat gcaattggca ttaacggaaa caatgccgaa acccaatcga
     1021 cccactacca accctatccc tatgataaag tcagtatatg gggaccattc cctcacaatt
     1081 taacgtaata tcttaattgt acgcaccgct gaaatgcgtt cagcgcgatc acggctgctg
     1141 acaggtaaaa atagcaacaa accacccgaa aagctgccgc gatcgcacct gataaatttt
     1201 aaccgtatgc atagctattc agccatgtga ataacgctgg ttttgcctgc gtaaacctca
     1261 tgacactgtt ttttttccat cttttcagtt gatgacatac gcagacatcg cgggatgagg
     1321 ctgaggaatg agcgcgatct ggcaaagagg caaaacacag caacaaaaac gacacgccag
     1381 aatcgcgccc ggatgcgttt ttaacgcgtt ccggtaccat ctggcaacct cccggaacaa
     1441 ctcaccgtca tatccctatt gacgggccac gccatacccg tgcttcccgt tcctgctctt
     1501 catgccagga ccgcgcacgc tcccgttcca ggcgtgcctg cctttcctgt tcatccctta
     1561 tctgctgttc gtgataaata accgactcaa gtggtccacc tgctcgacta atctctgcac
     1621 ctgctgactc aagtcgtcgc actgttccct cagttgcccg ttctcctgtc gtgtcagctc
     1681 gaacatatgc tgcaaatccg tgaaggcgct ctcccagtct ttcagccgct gcgtatagtc
     1741 ctgctgcaat tgctctaagg cgttcagtaa gtgcatttcc agctctgtca tactcactta
     1801 ctccctgacc agtcttactg cgttcttctt ctccaccgtc cagttgtttt cccccttcac
     1861 cccggacggc aataccagaa atttcccgtt cctgccctcg tgatacgtca caccccatgt
     1921 tttttcccgg agtttcgcca gcgtctcttc ctggtccctg atagccagga tgttcgccgc
     1981 aatccggctt tcctgccact gaatcagccc ccatgacgcc agaaaaatcc cgcccgtgac
     2041 gcaaagcgcc gtcagcgacg ggtacagtat ccgccctttg accagcttcc agagcagctc
     2101 ttcctgccgc cgggccagtt cgttctctgt ggcgctgaac tgcgcgttca cggcactgtt
     2161 cagcgtctcc agttgttctt tcaccgctgc tgtgtgtgcg ctgatagcgt ctctgatttt
     2221 ctgcccgttt aagttcagtt ccctgtctac agacgcttcg agcttcctga actcgctgtt
     2281 cagtatgttc tctgtagaga cggcacgctc tttcagtttc ttctcgaagt ctgtccccat
     2341 ttgtaaaaga ttgctcatac agcgcccctt tcagcctgag attacgcccg ccctccgggt
     2401 cggcgatact gatactgctc ctggttgtcc tcacaacctc aaaacctgcc gctgtaagcg
     2461 cctcagtgac atcctgacgc gtttttagcg ctccggcatg gtaaagagcc tccagtcccc
     2521 tcgtaatcgc ttctgcggcc tcctgtttcg ctttcggcag attattcggg gtgacaagtg
     2581 tccgcctgtt ctccggtgcg ttcgggtcgt gcagcccgta atggtgattc accagtgtct
     2641 gccaggcatt gattcgcgga cggtccgctc ggtcgtaata gggctggagc cgttttccgt
     2701 tcgccagctc catattcggg atgacaaaat tcagctcaag acgccccttg tcctggtgct
     2761 ccacccacag gatgctgtac tgattttttt caagaccggg catcagtacc cgctcaaagc
     2821 tctccatcac cctttcacgc tctcccggtg gcagggtctg ctctgcaaaa gacagaaccc
     2881 ccgaggtgta ttttttcgca aacggcgtgg catcgatgag ttcccgcacc tcttcgggag
     2941 caccccgcag aactctcgcc ccttcccggt tacgctcccg gcccagcagg taatcaaccg
     3001 gaccactgcc accgcctttt cccctggcat gaaacttaac tatcatcccg ctctccctgt
     3061 ttacggacct catccctcag ctcactcagt tcacgtccga tggccatcag tgcagccacc
     3121 acatgaaccc ggtcatgccc cgaccactgt ccgctgttta tcttccgggc tatctgattc
     3181 aggttattgc cgaccgaagc gaactggcgc aacagcggcg gtgccagtgt cggaagacct
     3241 gacgttttcg atggcggtgc ccccaggcag accttacgca tccatgacgc aagttgtttt
     3301 ccctcacaac gtgccagcag ccgcgcatgt tcctcatccg tgacccgtat cgtgagcatc
     3361 ctttcgcgtt tcaccggtat cattaaaaac ctccgacaga ctccccacac atggagaaac
     3421 agaactgtga ctaaacagga aaaaaccgcc cttaacatgg cccgcttcat cagaagccag
     3481 acgctgaccc tgctggaaaa actgaatgaa ctggacgccg acgaccaggc tgacatctgc
     3541 gaagcgcttc acgaccacgc tgacgagctt taccgcagct gcctcgcacg cttcggggat
     3601 gatggtgaaa acctctgaca catgcagctc ccggagacgg tcacagctgg cctgtgagcg
     3661 gatgccggga gcagacaagc ccgtcagggc gcgtcagcag gttttagcgg gtgtcggggc
     3721 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttact tatgcggcat
     3781 cagtgcggat tgtatgaaaa gtgcaccatg ccgggtgtga aatgccgcac agatgcgtaa
     3841 ggagaaaatg tagcttccgg cgctcttccg cttcctcgct cactgactcg ctacgctcgg
     3901 tcgttcggct gcggcgagcg gtatcagcgc actcaaagac ggtaacacag ttatccacag
     3961 aatcagggga taaagcaaaa agaaacatgt gagcaaaaaa caagagcccg aaaaaggccg
     4021 cgccggtggc gtttttccat aggctccgcc cccctgacga gcatc
//
