LOCUS       NZ_CP076310             2213 bp    DNA     circular CON 26-JUN-2023
DEFINITION  Escherichia coli strain Survcare253 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP076310
VERSION     NZ_CP076310.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19349419
            Assembly: GCF_018771465.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Nordmann,P., Yao,Y., Falgenhauer,L., Sadek,M., Imirzalioglu,C. and
            Chakraborty,T.
  TITLE     Recent emergence of aztreonam-avibactam resistance in NDM and OXA-48
            carbapenemase-producing Escherichia coli in Germany
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Yao,Y., Falgenhauer,L., Chakraborty,T. and Imirzalioglu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2021) Institute of Medical Microbiology,
            Justus-Liebig-University Giessen, Schubertstrasse 81, Giessen 35392,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 560x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/26/2023 02:24:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,013
            CDSs (total)                      :: 4,886
            Genes (coding)                    :: 4,617
            CDSs (with protein)               :: 4,617
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 82 of 269
            Pseudo Genes (incomplete)         :: 176 of 269
            Pseudo Genes (internal stop)      :: 47 of 269
            Pseudo Genes (multiple problems)  :: 35 of 269
            Pseudo Genes (short protein)      :: 4 of 269
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076310.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Survcare253"
                     /isolation_source="urine, catheter urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="Germany: Hesse"
                     /lat_lon="50.04 N 8.14 E"
                     /collection_date="2019"
                     /collected_by="IMM Giessen"
                     /note="Col4401"
     gene            329..592
                     /locus_tag="KNN07_RS24840"
                     /old_locus_tag="KNN07_24840"
     CDS             329..592
                     /locus_tag="KNN07_RS24840"
                     /old_locus_tag="KNN07_24840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_215619697.1"
                     /translation="MAPSCALLFLPFGLPVSFRCYGRVYLIPRLTLGSGKAVRSKLDCM
                     HEPPVQSDCCAFSGNYQLESNPERHDKAPLAAAIGNWISRAG"
                     /name="hypothetical protein"
     gene            1047..1472
                     /locus_tag="KNN07_RS24845"
                     /old_locus_tag="KNN07_24845"
     CDS             1047..1472
                     /locus_tag="KNN07_RS24845"
                     /old_locus_tag="KNN07_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676030.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_001676030.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIVPVLLRVWSTELALTNQRVIAKVGLIRRNTVELRIDKVESLGIHQGILGRIFNYGSI
                     VIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMDSKQQ"
                     /name="PH domain-containing protein"
     gene            complement(1739..1975)
                     /locus_tag="KNN07_RS24850"
                     /old_locus_tag="KNN07_24850"
     CDS             complement(1739..1975)
                     /locus_tag="KNN07_RS24850"
                     /old_locus_tag="KNN07_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001676031.1"
                     /translation="MNRLTNLTPAEKKFLDDAIAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSEFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          complement(2..100)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.0"
                     /coverage="95.0"
                     /evalue="1.27e-39"
     Typing          complement(2133..2211)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="93.0"
                     /evalue="6.569999999999999e-34"
     Typing          24..570
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.727"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 gcgaatatca gtgaagtgct tcatgtggca ggagaaaaaa ggcggcaccg gcgcgtcagc
       61 agaatatgcg atacaggata tattccgctt cctcgctcac tgactcgctg cgctcggtcg
      121 ttcggctgcg gcgagcggtg cgggcttact ggcggggcgg aaatttcctg gaagatgcca
      181 ggaagaaact taacagggaa gcgagaaggc cacggcgaag ccgtttttcc ataggctccg
      241 cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg
      301 acttaaagat accaggcgtt tccccctggt ggctccctcg tgcgctctcc tgttcctgcc
      361 tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tatctcattc cacgcctgac
      421 actcggttcc gggaaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
      481 gtccgactgc tgcgcctttt ccggtaacta tcaacttgag tccaacccgg aaagacacga
      541 caaagcgcca ctggcagcag ccattggtaa ctggattagc agagcgggat aatgtaggca
      601 gtgctacaga gttcttgaag tggtggcctg agtgcggcta cactggaagg acagtttagg
      661 tatctgcgct ctgctgaagc cagttaccac ggttagaaaa tctgccagga tttactaacc
      721 ttcgaaaaac cacctgccag ggtggttttt tcgtttccaa ggcaagagat tacccgcaga
      781 gaaagacgat ctcaagaaga tcatctgtga tattcgacag atatgggata aaggtttcta
      841 acgccaggag tatatggggc gtagcagcag cgttcaccac cgttgaatca agctactttc
      901 atggcaagaa ataaaagaac cgagcagtcc gagagtcgct acgctcccct taatttttgt
      961 atatctacca cgcggatata aggtttctat tgccgattca tcttaaaaag ggaaaaatcc
     1021 tattttttct aaatggatgt aaccttatgg gaagctacgt agaccaatca ttgacgagaa
     1081 atgaatctgt aatatctcgc gctcaaacct cctggattcc aacaatcatc ccagtaatta
     1141 tcggaatcct gttgctacct ttttacggct taggattact tataatagtc cctgtgcttc
     1201 ttagagtttg gtcaacggag cttgcattga ctaaccaaag agtcatcgca aaagtagggt
     1261 tgatccgacg taatacggtg gaactccgta ttgataaagt tgaaagcctt ggcatacatc
     1321 aagggatact aggacgaatc tttaattatg ggtcgatcgt aattaaaggt accggcggaa
     1381 ccaacacgcc aattccaaac atcaaggcac caatgcaatt cagatctatt gtgaacaatc
     1441 acattgagga aatggattct aagcagcagt agtttcctca taagcattac aaaaaaagcc
     1501 ccgcagggct ttttttgtac ctgccagtga gtcagattta ccataacatc cgttgtctgc
     1561 actgcctgag aggccctcag cgcgtttctg cggcctgaca gccgtttccg gcacctttca
     1621 ccgcaccacc ccctgaaaaa cgcccctgat gatttctgcg cgtctgcatg gctattcaca
     1681 ggagtgaatg gcgggcaatt tcggagtgtc agcaccacta aaccgccaaa aggcgcaatc
     1741 agcggcgcgg cgcgcgcgga cgtgaccagg caaactcgga ctgctgccgc tcatcctcac
     1801 gtaatgcacg ctggcggtcg gcgtaacgct gcgactcaat ctgcgcacgc gctctgttca
     1861 ggacgatatg ccggttcggc tgatttagct ttttgcctga cgctctctca gctgcggcaa
     1921 tagcatcatc aagaaatttt ttctctgcag gtgtcaggtt tgtcagtctg ttcatagcat
     1981 tctcccatca gaataacgcc gcacagtctg ccagacacag cagaaccgat gaagcgctgg
     2041 cggtgctgaa agacagccag aaacggtgaa cgggagcgga ttttaatccg gtgacgtgag
     2101 acgttgggca agccgcaggc gcgtcagtgg ttttagcggg tttcggggcg cagccctgaa
     2161 ccagtcacgc agcgctagcg gagtgtatac tggcttacta tgttggcacg gat
//
