LOCUS       NZ_CP076238             7323 bp    DNA     circular CON 17-NOV-2023
DEFINITION  Escherichia coli O157:H7 strain 146N4 plasmid p7, complete sequence.
ACCESSION   NZ_CP076238
VERSION     NZ_CP076238.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17295279
            Assembly: GCF_020423185.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Amadio,A.F., Bono,J.L., Irazoqui,J.M., Larzabal,M., Marques da
            Silva,W., Eberhardt,M.F., Riviere,N.A., Gally,D.L., Manning,S.D. and
            Cataldi,A.
  TITLE     Genomic analysis of hypervirulent Escherichia coli O157:H7 isolated
            from Argentinean cattle
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   Amadio,A.F., Bono,J.L., Irazoqui,J.M., Larzabal,M., Marques da
            Silva,W., Eberhardt,M.F., Riviere,N.A., Gally,D.L., Manning,S.D. and
            Cataldi,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2021) EEA Rafaela, Instituto Nacional de
            Tecnologia Agropecuaria, Ruta 34 Km 227, Rafaela, Santa Fe 2300,
            Argentina
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: HGAP v. 3
            Assembly Name                     :: Ecoli 146N4 assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200X
            Sequencing Technology             :: PacBio RSII; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/17/2023 07:58:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,521
            Genes (coding)                    :: 5,195
            CDSs (with protein)               :: 5,195
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 115 of 326
            Pseudo Genes (incomplete)         :: 194 of 326
            Pseudo Genes (internal stop)      :: 67 of 326
            Pseudo Genes (multiple problems)  :: 43 of 326
            Pseudo Genes (short protein)      :: 4 of 326
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076238.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="146N4"
                     /serotype="O157:H7"
                     /isolation_source="Rectal swab of cattle in slaughterhouse"
                     /db_xref="taxon:83334"
                     /plasmid="p7"
                     /geo_loc_name="Argentina: Pilar, Buenos Aires province"
                     /lat_lon="34.45 S 58.91 W"
                     /collection_date="2001-03"
                     /collected_by="Meichtri, L, ITA-INTA"
                     /genotype="eae, stx1, stx2c"
     gene            complement(join(7248..7323,1..188))
                     /locus_tag="JKN67_RS27600"
                     /old_locus_tag="JKN67_27395"
     CDS             complement(join(7248..7323,1..188))
                     /locus_tag="JKN67_RS27600"
                     /old_locus_tag="JKN67_27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038405.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038406.1"
                     /translation="MNKMAIIDLAKSFLASKITAMEFSERICVERRKLYGVKNLSQDVL
                     NCGEELFIAAERFEPDSDRADYEFDDNGLRIEVQSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            complement(185..2278)
                     /gene="cda"
                     /locus_tag="JKN67_RS27605"
                     /old_locus_tag="JKN67_27400"
     CDS             complement(185..2278)
                     /gene="cda"
                     /locus_tag="JKN67_RS27605"
                     /old_locus_tag="JKN67_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_069191907.1"
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSLGLAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRSGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPVSQLPAAGN
                     NVPVKYRLTDLMQNGTQYIAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGSGVSPL
                     YISTVEILDRNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKVELFSKAGVKQPPVY
                     TQEMVERANSVMNEQGTLVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVGPLLMRISLGALWFHSEDAGAGSDIVPGRDMEAMFSLNAQMLAGQGMVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNDSIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPAPQPSAPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDAGGLRDFIYWR
                     PDATGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTNNVVILKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            2536..2973
                     /locus_tag="JKN67_RS27610"
                     /old_locus_tag="JKN67_27405"
     CDS             2536..2973
                     /locus_tag="JKN67_RS27610"
                     /old_locus_tag="JKN67_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177063.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTGQDGRR
                     EFETSELIRAYGELKQDETAERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="hypothetical protein"
     gene            3057..3476
                     /locus_tag="JKN67_RS27615"
                     /old_locus_tag="JKN67_27410"
     CDS             3057..3476
                     /locus_tag="JKN67_RS27615"
                     /old_locus_tag="JKN67_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3961..4194)
                     /locus_tag="JKN67_RS27620"
                     /old_locus_tag="JKN67_27415"
     CDS             complement(3961..4194)
                     /locus_tag="JKN67_RS27620"
                     /old_locus_tag="JKN67_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4201..4761)
                     /locus_tag="JKN67_RS27625"
                     /old_locus_tag="JKN67_27420"
     CDS             complement(4201..4761)
                     /locus_tag="JKN67_RS27625"
                     /old_locus_tag="JKN67_27420"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001428444.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAALKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4793..5476)
                     /locus_tag="JKN67_RS28485"
                     /old_locus_tag="JKN67_27425"
                     /pseudo=""
     CDS             complement(<4793..5476)
                     /locus_tag="JKN67_RS28485"
                     /old_locus_tag="JKN67_27425"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5439..5762)
                     /locus_tag="JKN67_RS27635"
                     /old_locus_tag="JKN67_27430"
     CDS             complement(5439..5762)
                     /locus_tag="JKN67_RS27635"
                     /old_locus_tag="JKN67_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024177064.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGHWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5829..6020
                     /locus_tag="JKN67_RS27640"
                     /old_locus_tag="JKN67_27435"
     CDS             5829..6020
                     /locus_tag="JKN67_RS27640"
                     /old_locus_tag="JKN67_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165978.1"
                     /translation="MTKQEKTALNMARFIRSQTLILLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6690..6803
                     /locus_tag="JKN67_RS27645"
                     /old_locus_tag="JKN67_27440"
     CDS             6690..6803
                     /locus_tag="JKN67_RS27645"
                     /old_locus_tag="JKN67_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001428439.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDFTPLAAAIGN"
                     /name="hypothetical protein"
     gene            complement(7032..7178)
                     /locus_tag="JKN67_RS27650"
                     /old_locus_tag="JKN67_27445"
     CDS             complement(7032..7178)
                     /locus_tag="JKN67_RS27650"
                     /old_locus_tag="JKN67_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071527535.1"
                     /translation="MKKKIGVSIILVLVVLCLTACQNNYVRDVQGGTIAPSSSSKLTGV
                     AIQ"
                     /name="colicin release lysis protein"
     BGC             1..7278
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6106..6193)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          6216..6762
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.011"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3855..5449)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.12"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcagaatcag gctcgaaacg ctcagcagct ataaacaact cctccccaca attcaataca
       61 tcctgagaca aattcttaac accatacaat tttctccgct caacacaaat ccgctctgaa
      121 aactccattg ctgttatttt tgaagctaaa aatgacttcg ccaaatcgat tatcgccatc
      181 ttgttcatag ttcacctgtt tctaaataaa ttcgcccatt atccgcatct ggatttattt
      241 tccacccaga taagaactca ccatttgatt ttagtatcac cacattattt gtattaggat
      301 taaaataaac ttttgatcct ttttctcgtc ggtatgttcc tttctctact gtatccttat
      361 cggataaatg ttcctcaata gcatccctga atttagtaag agtttcacga ttctttttag
      421 tatcactaat accaaaatca ccagcatgtt tatatttttt atcaagctgt ttgcgtgaaa
      481 accgccctga atccagagga tcattaagca tcacatagac agcttcaaca ccagtcccgg
      541 tagcatcagg acgccagtaa ataaaatctc gaagtccacc ggcatcagag actggagtaa
      601 tcgtcgttac cggcatttca accggtttta cctccgtacc tgtatgcact ggggtaaccg
      661 gaacgggttg atgatttcct gtatctgttg gtgccgaagg ttgtggggcc ggatttacca
      721 gaatcgtcct ggaaggtgca ccgactactg ctggcagcgt aattttatcc agtcctgtag
      781 ctgtatcacg aacagcatta agaacaggaa ccgcagcagg gatactatca ttccctgtct
      841 tcagtaaatc cagagcaagt tgtccgttac tgtttatcaa ttgtccacga acaggcagat
      901 taacactcgt agcaccaggt tcaatgacca tgccctgtcc tgccaacatc tgagcattca
      961 gcgagaacat agcctccata tctcgccccg gcacgatatc acttcctgct ccggcgtctt
     1021 ctgaatggaa ccacagagcg ccaagagaaa tccgcataag caggggaccc acttcgggta
     1081 tggttacttt ctccagagca ttactgaaaa acttgcttat gttgcctgca atatcaccag
     1141 cagccgtcca gacaccagtt ccggtcatcg ccagttgtac agaactggca gtgttattca
     1201 gaacaagagt tccctgctca ttcattactg aattagccct ttccaccatt tcctgtgtat
     1261 atacaggagg ctgtttcaca cctgctttac taaataactc gacttttgct ttttcagctt
     1321 cggcccgggc taccgcttct tctttcttga ctctgaaatc atcctttgct ttattttctg
     1381 cttcctgacg ctgactcaga ccattacggt caagaatctc cacagttgaa atatataacg
     1441 gagaaacacc gctgccgtca ggaaaacgaa caatgacatc atgattatta ctggacatcg
     1501 tataacctgc ctcaacataa ttacctgtct cagcaataac gccctcttcc tgaatttcgg
     1561 gattagacag aaccggagta ttcatctgcc ccacacttaa taccaggtcc ggtagatttg
     1621 gtgatacagg ggcactgtaa acatctttaa tatttgttcc cggacggctc cggtccggaa
     1681 ccggaacggc atcaactacc ggcactgtca tcggaatacc tccgataata gcaatatatt
     1741 gcgtaccgtt ctgcataagg tccgtcagac gatatttcac cggtacatta ttacccgctg
     1801 ctggtaactg gctgacagga atatccgtta cctgctccag ttgctgtgta accataagac
     1861 gaataatttt ttgctcattt ccggaagcag gctccagacg ggcaaaagaa acagcagccg
     1921 ggcgtgatgg ttctggcaaa ccactacgta aaccattctc aagaactgtt cgtgcagcag
     1981 aaactttctc gtttccccag ggactatgca cactgacaga ccatcccgac cagtccccct
     2041 cttcaagttc cttatcaagg ataatccggc gaataattcc tggaaaaaga acactttgta
     2101 caagtgcgat accttgtgga ctgtaatctc cccatcccgg aggcatggat ggagcgagac
     2161 ctaatgaacc accgggtttc acatcaccgc ccggaattcg tccagttgac ggccacacaa
     2221 ccattgtgtc cccgtaatca ataccttctg tcgggacact accttcatta tcactcatag
     2281 aaaacctctt tgacattaaa aacaaatagt taaaagacgc atactgtata tacaaccact
     2341 ggttttatat acagtttaaa attacgccgc tgcgttttcc ctgtcaaccc tgtggatttt
     2401 cgtttttgtg aaaacgatca aaaaaacagc cacccacacc acgtgctatt cccacccgat
     2461 gccacaaaaa ccagcacaaa cattaccgtt ctccggcccc agtatgtttt actgaaacca
     2521 tgatatgaga caccaatggg acactgtcac ttcatggcat ggcacacact ccaggacgca
     2581 ctgaaaatga caggcagatc gcgttcacag ttttaccgtg atatgcgcgc aggccttgtc
     2641 agttaccgta ccggccagga cggacgacgg gagtttgaaa ccagtgaact gatccgggca
     2701 tacggcgaat taaagcagga tgagacagca gaaaggcaca gtgcgggaca ggcagaaaat
     2761 ccacatgatc agcagacaga acgcattctc cgggagctga atgagctgaa acaatgcctg
     2821 acactgatgc ttgaggacaa acaggcacag gatacggaac gccggcgcca ggaagccgaa
     2881 cgggaacagc tacataaaga gatagagcag ctcaggcagg cactggagca ggaaaaaaaa
     2941 cggggattct gggccagact gttcagccgg tgagcatcgg ctgcaagtga tgataagata
     3001 gtcagcggtt aaatataagt cactgagtac aaagatttaa aggtaaggag acactcatga
     3061 gacgttccgg cggcacactg tatcttatcg ccctgctgac agcggcaacg gtcctgacag
     3121 cctgcacgcc aaagggcagt gtggaacaac atacccggca ttacgtttat gcatcagacg
     3181 atggttttga tcctaacttt tccacccaga aggccgacac aacacgaatg atggtgcctt
     3241 ttttccggca gttctgggat atgggagcaa aggataaagc gacaggaaaa tcacggagtg
     3301 atgtgcagca acgcattcag cagtttcaca gccaggaatt tttaaactca ctccggggca
     3361 caactcaatt tgcgggtact gattaccgca gcaaagacct taccccgaaa aaatccaggc
     3421 tgctggctga gacgatttct gcggtttatc tcgatggcta cgagggaaga cagtaagtgg
     3481 atttaccata atcccttaat tgtacgcacc gctgaaatgc gttcagcgcg atcacggctg
     3541 ctgacaggta aaaatggcaa caaaccaccc gaaaagctgc cgcgatcgca cctgataaat
     3601 tttaaccgta tgcatagcta ttcagccatg tgaataacgc tggttttgcc tgcgtaaacc
     3661 tcatgacact gttttttttc catcttttca gttgatgaca tacgcagaca tcgcgggatg
     3721 aggctgagga atgagcgcga tctggcaaag aggcaaaaca cagcaacaaa aacgacacgc
     3781 cagaatcgcg cccggatgca tttttaacgc gttccggtac catctggcaa cctcccggaa
     3841 caactcaccg tcacatcccc attgacgggc cacgccatac ccgtacttcc cgttcctgct
     3901 cttcatgcca ggaccgcgca cgctcccgtt ccaggcgtgc cagcctttcc tgttcatccc
     3961 ttatctgctg ttcgtgataa ataaccgact caagcggtcc acctgcccgg ctaatctctg
     4021 cacctgctga ctcaagtcgt cgcactgttc cctcagttgc ccgttctcct gtcgtgtcag
     4081 ctcgaacata tgctgcaaat ccgtgaaggc gctctcccag tctttcagcc gctgcgtata
     4141 gtcctgctgc aattgctcta aggcgttcag taagtgcatt tccagctctg tcatactcac
     4201 ttactccctg accagtctta ctgcgttctt cttctccacc gtccagttgt tttccccctt
     4261 caccccggac ggcaatacca gaaatttccc gttcctgccc tcgtgatacg tcacacccca
     4321 tgttttttcc cggagtttcg ccagcgtctc ttcctggtcc ctgatagcca ggatgttcgc
     4381 cgcaatccgg ctttcctgcc actgaatcag cccccatgac gccagaaaaa tcccgcccgt
     4441 gacgcaaagc gccgtcagcg acgggtacag tatccgccct ttgatcagct tccagagcag
     4501 ctcttcctgc cgccgggcca gttcgttctc tgtggcgctg aactgcgcgt tcacggcact
     4561 gttcagcgtc tccagttgtt ctttcagcgc tgctgtgtgt gcgctgatag cgtctctgat
     4621 tttctgcccg tttaagttca gttccctgtc tacagacgct tcgagcttcc tgaactcgct
     4681 gttcagcatg ttctctgtag agacggcacg ctctttcagt ttcttctcga agtctgtccc
     4741 catttgtaaa agattgctca tacagcgccc ctttcagcct gagattacgc ccgccctccg
     4801 ggtcggcgat actgatactg ctcctggttg tcctcaccac ctcaaaacct gccactgtaa
     4861 gcgcctcagt gacatcctga cgcgttttta gcgctccggc atgataaaga gcctccagcc
     4921 ccctcgtaat cgcttctgcg gcctcctgtt tcgctttcgg cagattattc ggggtgacaa
     4981 gtgtccgcct gttctccggt gcgttcgggt cgtgcagccc gtaatgatga ttcaccagtg
     5041 tctgccaggc attgattcgc ggacggtccg cacggtcgta atagggctgg agccgttttc
     5101 cgctcaccag ctccatattc gggatgacaa aattcagctc aagacgcccc ttgtcctggt
     5161 gctccaccca caggatgctg tactgatttt tttcaagacc gggcatcagt acccgctcaa
     5221 agctctccat caccctttca cgctctcccg gtggcagtgt ctgctctgca aaagacagaa
     5281 cccccgaggt gtattttttc gcaaacggcg tggcatcgat gagttcccgc acctcttcgg
     5341 gatcaccccg cagaattctc gccccttccc ggttacgctc ccggcccagc aggtaatcaa
     5401 ccggaccact gccaccgcct tttcccctgg catgaaactt aactatcatc ccgttctccc
     5461 tgtttacgga cctcatccct cagctcactc agttcacgtc cgatggccat cagtgcagcc
     5521 accacatgaa cccggtcatg ccccgaccag tgtccgctgt ttatcttccg ggctatctga
     5581 ttcaggttat tgccgaccga agcgaactgg cgcaacagcg gcggtgccag tgtcggaaga
     5641 cctgacgttt tcgatggcgg tgcacccagg cagaccttac gcatccatga cgcaagttgt
     5701 tttccctcac aacgtgccag cagccgcgca tgttcctcat ccgtgacccg tatcgtgagc
     5761 atcctttcgc gtttcaccgg tatcattaaa aacctccgac agactcccca cacatggaga
     5821 acagaactgt gactaaacag gaaaaaacag cccttaacat ggcccgcttt atcagaagcc
     5881 agacgctgat cctgctggaa aaactgaatg aactggacgc cgacgaccag gcagacatct
     5941 gcgaagccct tcacgaccac gccgacgagc tttaccgcag ctgcctcgca cgcttcgggg
     6001 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgcctgtgag
     6061 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggttttagc gggtgtcggg
     6121 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta atcgtgtggc
     6181 atcagtgagg attgtatgaa aagtgcacca tgtacggtgt gaaatgccgc acagatgtgt
     6241 aaggagaaca tgcagatgcc gatgctcttc cgcttcctcg ctcactgact cgctgtgctc
     6301 ggtcgttcgg ctgcggcgag cggtgtctgc tcacacaaaa gcggcaatac tgttatccac
     6361 agaatcaggg gataacgccg gaaagaacat gtgagcaaca agacaaagcc ggggaaaagg
     6421 ccgcgcaggt ggcgcttttt ccataggctc cgcccccctg acgagcatca caaaaaccga
     6481 cgctcaagtc agaggtggcg aaacccgaca ggacttaaag ataccaggcg tttccccccg
     6541 gaagctccct cgtgcgctct cctgttccga ccctgccgat taccggatac ctgtccgcct
     6601 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgttggtat ctcagttcgg
     6661 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6721 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac tttacgccac
     6781 tggcagcagc cattggtaac tgaaaagtgg atttagatac gcagaactct tgaagttgaa
     6841 gccttatcgc ggctacactg aaaggacagc atttggtatc tgtgctccac taaagccagt
     6901 taccttcgga aaaagagttg gtagctcttg ttccggcaaa caaaccaccg ttggtagcgg
     6961 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7021 tttaatcttt ttcactgaat cgctaccccg gttagtttcg aggatgatga tggagcaatc
     7081 gttcctccct gaacatccct tacatagtta ttttgacaag cagtcagaca caaaacaaca
     7141 agaacgagaa taatcgatac ccctatcttt tttttcataa catctccttg tcatgagtga
     7201 gtatctgaca tttataatta aattccagat attaaaatca ctcatattta taatttaaat
     7261 ttttccagga tagattggac ttcaatccta agtccattat catcgaattc ataatcagcc
     7321 cta
//
