LOCUS       NZ_CP076109             9642 bp    DNA     circular CON 20-JUL-2023
DEFINITION  Klebsiella pneumoniae strain KpIM03_1 plasmid pKpIM03_1_3, complete
            sequence.
ACCESSION   NZ_CP076109
VERSION     NZ_CP076109.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17976209
            Assembly: GCF_030503755.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Tang,N.
  TITLE     In vivo evolution of carbapenem resistance in hypervirulent
            Klebsiella pneumoniae in long-term treatment patient
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   Tang,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2021) State Key Laboratory of Microbial Resources,
            Institute of Microbiology, Chinese Academy of Sciences, No.1 Beichen
            West Road, Beijing 100000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/18/2023 16:08:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,637
            CDSs (total)                      :: 5,514
            Genes (coding)                    :: 5,361
            CDSs (with protein)               :: 5,361
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 79 of 153
            Pseudo Genes (incomplete)         :: 79 of 153
            Pseudo Genes (internal stop)      :: 21 of 153
            Pseudo Genes (multiple problems)  :: 23 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076109.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9642
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KpIM03_1"
                     /isolation_source="sputum"
                     /host="patient"
                     /db_xref="taxon:573"
                     /plasmid="pKpIM03_1_3"
                     /geo_loc_name="China"
                     /collection_date="2019-03-10"
     gene            1352..2650
                     /locus_tag="KM293_RS28150"
                     /old_locus_tag="KM293_28050"
     CDS             1352..2650
                     /locus_tag="KM293_RS28150"
                     /old_locus_tag="KM293_28050"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_040196943.1"
                     /translation="MIVNFSLSNAFSFKDAQNLSMLAVSSPKDDINQANVMSIKGERIL
                     KSALIFGANASGKSNFIKTISIMKDIVLHSVQAIESSVTKNIIPFLLDEDGPKSPTEME
                     VIFLKDNIKYRYGLSLLQGKIKEEWLYYTPSSRETMLFERDGLDIDVNRASFGESALFI
                     KDKIIQKTRDDVPFISVIAGFNGEHSKRVVDWFRNIVVISGSNEGAFTGITLHLINESP
                     DFKLWLKKILPSFQISDIYLDEVAPDIPFNDIKTDDDNLKSLFSSIQNLAKTQKMFSIR
                     VIKKTANGEISVPIDFESEGTKKIVYMLGPIYNAINNGNLLLVDEFDSKFHTLLSKFIF
                     RMFHEQSEHGSQIIAAVQDVNLMSIDCFRRDQIWFVDKNYETGGSNLYSLVEYKERQRS
                     LKPSYGADYLNGDFDAIPLFENPQDIETLMSKE"
                     /name="ATP-binding protein"
     gene            2655..3095
                     /locus_tag="KM293_RS28155"
                     /old_locus_tag="KM293_28055"
     CDS             2655..3095
                     /locus_tag="KM293_RS28155"
                     /old_locus_tag="KM293_28055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_046654978.1"
                     /translation="MSPKRSRSLKSSSRKEETKDSKPKSESTDRFISVSFKYFCNVDDV
                     GQSIDSWRDDGCLGELVEKLIYLTANGITKVMSDEIFTNYNRYPDSSVNDFKCPDGLSK
                     DESWGVIKNIGGQKRRVAGFLRDNVFYIVFFDRDHRFWKSNK"
                     /name="hypothetical protein"
     gene            3860..4051
                     /locus_tag="KM293_RS28160"
                     /old_locus_tag="KM293_28060"
     CDS             3860..4051
                     /locus_tag="KM293_RS28160"
                     /old_locus_tag="KM293_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_040196939.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EYLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4051..4452
                     /locus_tag="KM293_RS28165"
                     /old_locus_tag="KM293_28065"
     CDS             4051..4452
                     /locus_tag="KM293_RS28165"
                     /old_locus_tag="KM293_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_040196941.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPEPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            6161..7459
                     /locus_tag="KM293_RS28170"
                     /old_locus_tag="KM293_28070"
     CDS             6161..7459
                     /locus_tag="KM293_RS28170"
                     /old_locus_tag="KM293_28070"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_040196943.1"
                     /translation="MIVNFSLSNAFSFKDAQNLSMLAVSSPKDDINQANVMSIKGERIL
                     KSALIFGANASGKSNFIKTISIMKDIVLHSVQAIESSVTKNIIPFLLDEDGPKSPTEME
                     VIFLKDNIKYRYGLSLLQGKIKEEWLYYTPSSRETMLFERDGLDIDVNRASFGESALFI
                     KDKIIQKTRDDVPFISVIAGFNGEHSKRVVDWFRNIVVISGSNEGAFTGITLHLINESP
                     DFKLWLKKILPSFQISDIYLDEVAPDIPFNDIKTDDDNLKSLFSSIQNLAKTQKMFSIR
                     VIKKTANGEISVPIDFESEGTKKIVYMLGPIYNAINNGNLLLVDEFDSKFHTLLSKFIF
                     RMFHEQSEHGSQIIAAVQDVNLMSIDCFRRDQIWFVDKNYETGGSNLYSLVEYKERQRS
                     LKPSYGADYLNGDFDAIPLFENPQDIETLMSKE"
                     /name="ATP-binding protein"
     gene            7464..7904
                     /locus_tag="KM293_RS28175"
                     /old_locus_tag="KM293_28075"
     CDS             7464..7904
                     /locus_tag="KM293_RS28175"
                     /old_locus_tag="KM293_28075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_046654978.1"
                     /translation="MSPKRSRSLKSSSRKEETKDSKPKSESTDRFISVSFKYFCNVDDV
                     GQSIDSWRDDGCLGELVEKLIYLTANGITKVMSDEIFTNYNRYPDSSVNDFKCPDGLSK
                     DESWGVIKNIGGQKRRVAGFLRDNVFYIVFFDRDHRFWKSNK"
                     /name="hypothetical protein"
     gene            8693..8884
                     /locus_tag="KM293_RS28180"
                     /old_locus_tag="KM293_28080"
     CDS             8693..8884
                     /locus_tag="KM293_RS28180"
                     /old_locus_tag="KM293_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_040196939.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EYLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8884..9285
                     /locus_tag="KM293_RS28185"
                     /old_locus_tag="KM293_28085"
     CDS             8884..9285
                     /locus_tag="KM293_RS28185"
                     /old_locus_tag="KM293_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_040196941.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPEPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(3332..3739)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.902"
                     /coverage="99.0"
                     /evalue="2.74e-174"
     Typing          complement(8155..8545)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.284"
                     /coverage="91.0"
                     /evalue="2.43e-115"
ORIGIN
        1 tggtgagtcg ttcggcgact tgccataacg tgattgccgg ccgtcgacga acccgattcc
       61 tgaacgtgct gaaaaccggt tttccggctg cgccggcaac gcttcacggc cacgcaataa
      121 attgcgtccc cgttcaccgt tttcggatgg gaagaacgcc ggaaacagga cttaccctgg
      181 ctgaatggcg tgaattttcg cgctatgcac ttgcgtgcat actcatgcat gccgtaaaaa
      241 cagagcctgc gcgtttctgg cgggttttcg ggtggtttgt tgcctgtttt accggtttcc
      301 cgtcagaaac gccctgaggc cgttttagcg gtgcgtacaa ttagggtgtt atggtaaata
      361 gtcgtcggct acaggcataa aaaaaagccc cgaggggctt tttttttact tatcagtggc
      421 tttaaccaat agcttccact tattgctatg cccttttttc ttacagttag agcagggtgg
      481 aagcttgatg cattcccggt tcacgacgtc ttcgtattta caagtctggc actcataaat
      541 gccgggactg ggaggtacat cgccgatttc acgtaggact cggtattgat caggcttgtc
      601 agtttcaaga actgaacgct tgttttcata gaatgccatt ctgttccctt acatagataa
      661 aatctgggtt cgcagcctat tacggctgcg ggttttgtta ttttttagga tcacgtttta
      721 ctggtatctt aaacatccca ccgtttggac gggtgatccg cttacccttg actgttatgt
      781 aagggcgaaa aatgtatgcc tgagactcgt ctgtctgcat ggtgcatttc ctgtttttcg
      841 ccacatgaat gttgattttt gctatcaaaa ttgtactgta gcaaccaaca aatctagtag
      901 cttgcttgta aaagcacttg aggtggtact caagcgccgc gtttgcgtat ggtttgttgg
      961 ggttagttct ggtggtacag gctaaccctt tttattacat tgaaatctgc attcatcctc
     1021 ctgaattata tggttaattt ttaattcgca atgtgctttt tcacagtgtc tgcaccgctg
     1081 agcataaacg cgataaacag aaaccatccc cacccatcga ttgaatgaaa tgccaaaact
     1141 gctgagctaa tgaccgcagc gaatggcacc atctgatata gaaataacct catttttact
     1201 cctgttagca ctcagcattt tcaatgcctt tagttagtac tctgtaacac ttcacatgct
     1261 tgtatttgca tgctcacatg ctattcttaa tgctgttttt tgtcaacaaa tgacaaaatt
     1321 ttggtttaag aacgcaaaaa tggatgtcct tatgattgtt aacttcagct tgtctaatgc
     1381 attctcgttc aaagatgctc aaaatttgag catgttggcg gtgtcatcgc caaaggatga
     1441 catcaatcag gctaatgtca tgtctatcaa aggtgaaagg atattaaagt ccgccttgat
     1501 ttttggtgcg aatgcgagtg ggaaatcaaa ttttataaag acaatatcta ttatgaaaga
     1561 tattgttttg cattccgttc aggctataga atcaagtgtc acaaaaaaca taataccttt
     1621 tctgcttgat gaggatggcc caaaaagtcc aactgaaatg gaagttatct ttttaaagga
     1681 taacattaaa tacaggtatg ggttatcctt acttcaaggt aaaattaaag aggaatggct
     1741 ttactataca ccatcatcta gagaaacaat gttgtttgag agagatggtt tagatattga
     1801 tgtaaacaga gcttcctttg gggagtcggc tttgtttatt aaagataaga ttattcagaa
     1861 aaccagggat gatgttccat ttatatctgt tattgctggg tttaacggtg aacactctaa
     1921 aagagtcgtg gattggttta ggaatattgt tgttatctct ggctcaaatg aaggtgcatt
     1981 tacagggatt acattacatc ttattaatga gtctcctgac tttaaattgt ggctaaaaaa
     2041 aatactcccg tcatttcaga ttagtgatat ttatttagat gaggtcgccc cagacattcc
     2101 ttttaatgac ataaaaacgg acgacgataa tttaaaaagt ctatttagct ccatacaaaa
     2161 tttagcaaaa acgcaaaaaa tgttttctat tcgtgtgatt aagaaaacag cgaatggtga
     2221 aatatccgtc cctattgatt ttgaatcaga gggaaccaag aaaatagtat atatgcttgg
     2281 ccctatttat aatgccatta acaatggtaa cttattgtta gttgatgagt ttgactctaa
     2341 attccatact ttgctttcta agtttatatt tagaatgttt catgagcaaa gcgaacatgg
     2401 tagccagatt atagctgctg ttcaggacgt taacctgatg tctatagatt gcttcaggag
     2461 agatcaaatt tggtttgttg ataaaaacta tgagactggt ggaagtaact tgtattcact
     2521 tgttgaatat aaggaaaggc aaagatcttt gaagccatcc tatggggctg attacttaaa
     2581 tggtgacttt gatgcaatac cgttgtttga aaatccacaa gatattgaaa ccttaatgtc
     2641 taaggaatga tgtcatgtca ccaaagagaa gtagaagcct aaaaagctcg tcaagaaagg
     2701 aagaaactaa agactccaag cctaaatccg agtcaacgga taggtttatt tctgtgagtt
     2761 tcaagtactt ctgtaatgtt gatgatgttg gtcaatctat agattcctgg cgcgatgatg
     2821 gatgccttgg tgagttagtg gaaaaactaa tttatttgac tgccaatggt attactaaag
     2881 taatgtctga tgagatcttt actaattaca atcgctatcc agattcttct gttaacgact
     2941 tcaagtgccc cgatggatta tcaaaagatg aatcttgggg ggttataaaa aacattggcg
     3001 ggcagaaaag aagggttgct ggattccttc gggataatgt tttttatatt gttttctttg
     3061 atagggacca tcggttttgg aagtcaaata agtaatgtat tgccttgagt ggttctcttt
     3121 ttacaaggat cctcttgaga tcagaggcta ctcatcgtaa tctcttgctt cgaaaacgaa
     3181 aaaaccgcct tggcgggcgg tttttcgaag gttccaagag ttggcgctct ttgaaccgag
     3241 gtaactggct tatgaggagc acagtaacca aaacttgttc tttcagttta gccttaaccg
     3301 gcacataact tcaagactaa ctcctctaaa tcagttacca gtggctgctg ccagtggtgc
     3361 ttttgcatgt cttaccgggt tggactcaag atgatagtta ccggatcaag gcgcagtagt
     3421 cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc ttcccggaac
     3481 tgagtgtcag gcgtggaatg agataaacgc ggccataact gcggaatgac accggtaaac
     3541 cgaatggcag gaacaggaga gcgcacgagg gagccatcag ggggaaacgc ctggtatctt
     3601 tatagtcctg tcgggtttcg ccgccactga tttgagcgtc agattctgtg atgctcgtca
     3661 ggggggcgga gcctatggaa aaacggccgc tgtgcggcct ttccttttct cctcctttga
     3721 ccgctcggtt ttttcgagct tttatacgga tgctcctgcc gctcgccgca gccgaacgac
     3781 cggagcgtag cgactgagtg agcgaggaag cggaaaagag aacatcccta cactgagcac
     3841 tgacgctgtg aggcctctta tgacgaaaca acagaaaaca gcgctgaata tggctaaatt
     3901 catccagaat cagtctctgc tcctgctgga gaagcttaac gagcttgatc tagatgtcga
     3961 ggccgacctt tgtgagaaac tccacgatga tgcggagtat cttttccgta cactgtcctc
     4021 gagactggat gcattacagg atggtaactg atggccagag tgtctattag tgaggctgcc
     4081 cgactgacgg gtaagagtag aacaacactg cacaggttaa ttaaagcagg tgacctgtcc
     4141 acctgtagcg gtgagcgcaa tgcaaaaatg gtagatactt cagagctttt acgtgtcttt
     4201 ggtccctttg agcaaccgaa gggtgaacac cattctggac aggtctctga acagcgtgat
     4261 acagcctctg ctgaccagag tgaacaggtg attcgtcatc tgaaacagga gattgaacac
     4321 ctgaacacct tacttttgtc caaggattcg cacattgaca gcctgaaaca ggccatgctc
     4381 cttcttgaaa atcaccaggc aaaattaact gaatccccgg agccatggtg gaaattctgg
     4441 aaaaaatcct gatcctgcca tgcttgagtt ttcctctccg gccatcccgg ggagttcctg
     4501 aaccttccca aaaatcgcca gatctctgaa cgctcctcac gactaaaaat acccacacac
     4561 tacgcaaaat taaaattttg cagatttgta tttaatatca gttgtttact ttcctgaaat
     4621 ttcgtttttt aaattttatt tacatagatc cgactctatg acgactcgtc atagagtcgg
     4681 taacgactca ggactataaa aacaatgagt taataacgac ccgactctag acacgactcc
     4741 ccggttggtt gttttttaga cgcggcgact ctgggtacga ctctagtgaa gagtcgccgg
     4801 tgaatcgtgt ggtgagtcgt tcggcgactt gccataacgt gattgccggc cgtcgacgaa
     4861 cccgattcct gaacgtgctg aaaaccggtt ttccggctgc gccggcaacg cttcacggcc
     4921 acgcaataaa ttgcgtcccc gttcaccgtt ttcggatggg aagaacgccg gaaacaggac
     4981 ttaccctggc tgaatggcgt gaattttcgc gctatgcact tgcgtgcata ctcatgcatg
     5041 ccgtaaaaac agagcctgcg cgtttctggc gggttttcgg gtggtttgtt gcctgtttta
     5101 ccggtttccc gtcagaaacg ccctgaggcc gttttagcgg tgcgtacaat tagggtgtta
     5161 tggtaaatag tcgtcggcta caggcataaa aaaaagcccc gaggggcttt ttttttactt
     5221 atcagtggct ttaaccaata gcttccactt attgctatgc ccttttttct tacagttaga
     5281 gcagggtgga agcttgatgc attcccggtt cacgacgtct tcgtatttac aagtctggca
     5341 ctcataaatg ccgggactgg gaggtacatc gccgatttca cgtaggactc ggtattgatc
     5401 aggcttgtca gtttcaagaa ctgaacgctt gttttcatag aatgccattc tgttccctta
     5461 catagataaa atctgggttc gcagcctatt acggctgcgg gttttgttat tttttaggat
     5521 cacgttttac tggtatctta aacatcccac cgtttggacg ggtgatccgc ttacccttga
     5581 ctgttatgta agggcgaaaa atgtatgcct gagactcgtc tgtctgcatg gtgcatttcc
     5641 tgtttttcgc cacatgaatg ttgatttttg ctatcaaaat tgtactgtag caaccaacaa
     5701 atctagtagc ttgcttgtaa aagcacttga ggtggtactc aagcgccgcg tttgcgtatg
     5761 gtttgttggg gttagttctg gtggtacagg ctaacccttt ttattacatt gaaatctgca
     5821 ttcatcctcc tgaattatat ggttaatttt taattcgcaa tgtgcttttt cacagtgtct
     5881 gcaccgctga gcataaacgc gataaacaga aaccatcccc acccatcgat tgaatgaaat
     5941 gccaaaactg ctgagctaat gaccgcagcg aatggcacca tctgatatag aaataacctc
     6001 atttttactc ctgttagcac tcagcatttt caatgccttt agttagtact ctgtaacact
     6061 tcacatgctt gtatttgcat gctcacatgc tattcttaat gctgtttttt gtcaacaaat
     6121 gacaaaattt tggtttaaga acgcaaaaat ggatgtcctt atgattgtta acttcagctt
     6181 gtctaatgca ttctcgttca aagatgctca aaatttgagc atgttggcgg tgtcatcgcc
     6241 aaaggatgac atcaatcagg ctaatgtcat gtctatcaaa ggtgaaagga tattaaagtc
     6301 cgccttgatt tttggtgcga atgcgagtgg gaaatcaaat tttataaaga caatatctat
     6361 tatgaaagat attgttttgc attccgttca ggctatagaa tcaagtgtca caaaaaacat
     6421 aatacctttt ctgcttgatg aggatggccc aaaaagtcca actgaaatgg aagttatctt
     6481 tttaaaggat aacattaaat acaggtatgg gttatcctta cttcaaggta aaattaaaga
     6541 ggaatggctt tactatacac catcatctag agaaacaatg ttgtttgaga gagatggttt
     6601 agatattgat gtaaacagag cttcctttgg ggagtcggct ttgtttatta aagataagat
     6661 tattcagaaa accagggatg atgttccatt tatatctgtt attgctgggt ttaacggtga
     6721 acactctaaa agagtcgtgg attggtttag gaatattgtt gttatctctg gctcaaatga
     6781 aggtgcattt acagggatta cattacatct tattaatgag tctcctgact ttaaattgtg
     6841 gctaaaaaaa atactcccgt catttcagat tagtgatatt tatttagatg aggtcgcccc
     6901 agacattcct tttaatgaca taaaaacgga cgacgataat ttaaaaagtc tatttagctc
     6961 catacaaaat ttagcaaaaa cgcaaaaaat gttttctatt cgtgtgatta agaaaacagc
     7021 gaatggtgaa atatccgtcc ctattgattt tgaatcagag ggaaccaaga aaatagtata
     7081 tatgcttggc cctatttata atgccattaa caatggtaac ttattgttag ttgatgagtt
     7141 tgactctaaa ttccatactt tgctttctaa gtttatattt agaatgtttc atgagcaaag
     7201 cgaacatggt agccagatta tagctgctgt tcaggacgtt aacctgatgt ctatagattg
     7261 cttcaggaga gatcaaattt ggtttgttga taaaaactat gagactggtg gaagtaactt
     7321 gtattcactt gttgaatata aggaaaggca aagatctttg aagccatcct atggggctga
     7381 ttacttaaat ggtgactttg atgcaatacc gttgtttgaa aatccacaag atattgaaac
     7441 cttaatgtct aaggaatgat gtcatgtcac caaagagaag tagaagccta aaaagctcgt
     7501 caagaaagga agaaactaaa gactccaagc ctaaatccga gtcaacggat aggtttattt
     7561 ctgtgagttt caagtacttc tgtaatgttg atgatgttgg tcaatctata gattcctggc
     7621 gcgatgatgg atgccttggt gagttagtgg aaaaactaat ttatttgact gccaatggta
     7681 ttactaaagt aatgtctgat gagatcttta ctaattacaa tcgctatcca gattcttctg
     7741 ttaacgactt caagtgcccc gatggattat caaaagatga atcttggggg gttataaaaa
     7801 acattggcgg gcagaaaaga agggttgctg gattccttcg ggataatgtt ttttatattg
     7861 ttttctttga tagggaccat cggttttgga agtcaaataa gtaatgtatt gccttgagtg
     7921 gttctctttt tacaaggatc ctcttgagat cagaggctac tcatcgtaat ctcttgcttc
     7981 gaaaacgaaa aaaccgcctt gaggaggcgg tttgatcgaa ggttaagtca gttcagggaa
     8041 ctgcttaacc gggtaactgg gtatacggac cacccagata ccaaaactgt tctttcagtg
     8101 tagccgcagt taggcaccca cttcaaacct ctcaatatct cgtatatatc cagttaccaa
     8161 tggctgctgc cagtggcgtt tttgcgtgtc cgtccgggtt ggactcaaga tgatagttac
     8221 cggaaagggc gcagcagtcg ggctgaacag gggggttcgt gcatacagcc cagcttggaa
     8281 cgaacgacct aaaccgagcc gagataccag cagcgtgagc tatgaagcgc cacgcttccc
     8341 gaaaggaaaa aggcggaaca ggtatccggt aaacggcagg aatcggaaca ggagagcgca
     8401 cgaggccgcc ggagagaaat caccggcatc tttaagtcct gtcgggtttc gtcctcctct
     8461 gacttgagcg tccatttctg tgatgctcgt cagggaggcg gaactatgga aaaacagcaa
     8521 cgccgcatcc ttttctccgg ccctttctga tttgagcgcc tttgctttat cccgttatcc
     8581 gtgagtcggt cacgcccgcc gcagccgaac gaccggagcg tagcgactga gtgagcgagg
     8641 aagcggaaaa gagaacatcc ctacactgag cactgacgct gtgaggcctc ttatgacgaa
     8701 acaacagaaa acagcgctga atatggctaa attcatccag aatcagtctc tgctcctgct
     8761 ggagaagctt aacgagcttg atctagatgt cgaggccgac ctttgtgaga aactccacga
     8821 tgatgcggag tatcttttcc gtacactgtc ctcgagactg gatgcattac aggatggtaa
     8881 ctgatggcca gagtgtctat tagtgaggct gcccgactga cgggtaagag tagaacaaca
     8941 ctgcacaggt taattaaagc aggtgacctg tccacctgta gcggtgagcg caatgcaaaa
     9001 atggtagata cttcagagct tttacgtgtc tttggtccct ttgagcaacc gaagggtgaa
     9061 caccattctg gacaggtctc tgaacagcgt gatacagcct ctgctgacca gagtgaacag
     9121 gtgattcgtc atctgaaaca ggagattgaa cacctgaaca ccttactttt gtccaaggat
     9181 tcgcacattg acagcctgaa acaggccatg ctccttcttg aaaatcacca ggcaaaatta
     9241 actgaatccc cggagccatg gtggaaattc tggaaaaaat cctgatcctg ccatgcttga
     9301 gttttcctct ccggccatcc cggggagttc ctgaaccttc ccaaaaatcg ccagatctct
     9361 gaacgctcct cacgactaaa aatacccaca cactacgcaa aattaaaatt ttgcagattt
     9421 gtatttaata tcagttgttt actttcctga aatttcgttt tttaaatttt atttacatag
     9481 atccgactct atgacgactc gtcatagagt cggtaacgac tcaggactat aaaaacaatg
     9541 agttaataac gacccgactc tagacacgac tccccggttg gttgtttttt agacgcggcg
     9601 actctgggta cgactctagt gaagagtcgc cggtgaatcg tg
//
