LOCUS       NZ_CP076014             4167 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WGR351 plasmid
            p6WGR351, complete sequence.
ACCESSION   NZ_CP076014
VERSION     NZ_CP076014.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18317431
            Assembly: GCF_018623335.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Nicholas,L., Sunil,K., Mohammad,F., Prashant,J.R., Balaji,V. and
            Yamuna,B.D.
  TITLE     Complete genome of Klebsiella pneumoniae_WGR351
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Nicholas,L., Sunil,K., Mohammad,F., Prashant,J.R., Balaji,V. and
            Yamuna,B.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: CANU v. v.1.7; UNICYCLER HYBRID v. v.0.4.6
            Assembly Name                     :: WGR351
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 133.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:02:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,689
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,440
            CDSs (with protein)               :: 5,440
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 46 of 125
            Pseudo Genes (incomplete)         :: 71 of 125
            Pseudo Genes (internal stop)      :: 22 of 125
            Pseudo Genes (multiple problems)  :: 12 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076014.1.
            Bacteria and source DNA available from Wockhardt Research Centre.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WGR351"
                     /isolation_source="Pus"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p6WGR351"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2017"
                     /collected_by="Wockhardt Research Centre"
     gene            join(4066..4167,1..141)
                     /locus_tag="J4H97_RS28230"
                     /old_locus_tag="J4H97_28230"
     CDS             join(4066..4167,1..141)
                     /locus_tag="J4H97_RS28230"
                     /old_locus_tag="J4H97_28230"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(487..888)
                     /locus_tag="J4H97_RS28235"
                     /old_locus_tag="J4H97_28235"
     CDS             complement(487..888)
                     /locus_tag="J4H97_RS28235"
                     /old_locus_tag="J4H97_28235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(888..1079)
                     /locus_tag="J4H97_RS28240"
                     /old_locus_tag="J4H97_28240"
     CDS             complement(888..1079)
                     /locus_tag="J4H97_RS28240"
                     /old_locus_tag="J4H97_28240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1495..1608
                     /locus_tag="J4H97_RS28245"
                     /old_locus_tag="J4H97_28245"
                     /pseudo=""
     CDS             1495..1608
                     /locus_tag="J4H97_RS28245"
                     /old_locus_tag="J4H97_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1953..3068
                     /locus_tag="J4H97_RS28250"
                     /old_locus_tag="J4H97_28250"
     CDS             1953..3068
                     /locus_tag="J4H97_RS28250"
                     /old_locus_tag="J4H97_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_096768689.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILKN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            3068..3700
                     /locus_tag="J4H97_RS28255"
                     /old_locus_tag="J4H97_28255"
     CDS             3068..3700
                     /locus_tag="J4H97_RS28255"
                     /old_locus_tag="J4H97_28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     Typing          1201..1608
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 cgtgaagcgt tgccggcgca gccggaaaac cggttttcag caccttcagg aatcgggttc
       61 gtcgacggcc ggcaatcacg ttatggcaag tcgccggacg actcaccaca cgattcaccg
      121 gcgattcgaa tcgtgaattg aatcaagagt caccatgagc aaaaaacacc caaagtgatg
      181 aattgaatca agagtcgatt catcataaca atatgataat aaagcgtgat tcacatatga
      241 atcgtgggca attcgtcagc gattcgccat agagtcgtcc acgacttgga tctgcacttt
      301 gtaacacatc accgcacatt tatttttcat taacccattg ttgtaaataa ggaatctgca
      361 aaattttaat tttgcgtggg gtgtgggtat ttttagtggt gaggagcgtt cagagatctg
      421 acgatttttg ggaaggttca ggaactccct gggatggccg gagaggaaaa ctgaagcatg
      481 acggggtcag gattttttcc agaatttcca ccatggcgcc ggggattcag ttaattttgc
      541 ctggtgattt tcaagaagga gcatggcctg tttcaggctg tcaatgtgcg aatccttgga
      601 caaaagtaag gtgttcaggt gttcaatctc ctgtttcaga tgacggatca cctgttcact
      661 ctggtcagca gaggctgtat cacgctgtcc agagacctgt ccagaatggt gttcaccctt
      721 cggttgctca aaggggccaa agacacgtaa aagctctgaa gtatctacca tttttgcatt
      781 gcgctcaccg ctacaggtgg acaggtcacc tgctttaatt aacctgtgca gtgttgttct
      841 actcttaccc gtcagtcggg cagcctcact aatagacact ctggccatca gttaccatcc
      901 tgtaatgcat ccagtctcga ggacagtgta cggaaaagat gctccgcatc atcgtggagt
      961 ttctcacaaa ggtcggcctc gacatcgaga tcaagctcgt taagcttctc cagcaggagc
     1021 agagactgat tctggatgaa tttagccatg ttcagcgctg ttttctgttg tttcgtcata
     1081 agaggcctca gagcgtcagt ggtcagtgta gggatctcct cttttccgct tcctcgctca
     1141 ctcagtcgct acgctcctgc cgttcggctg cggcgagcgg caggagcatg cgtagaaacg
     1201 ctcgagaaaa ccgagtaatc gcatggagga aagaaggaaa ggccgcacag cggccgtttt
     1261 tccataggct ccgcccccct gacgagcatc acggaatctg acgctcaaat cagtggtggc
     1321 gaaacccgac aggactataa agataccagg cgtttccccc tgatggctcc ctcgtgcgct
     1381 ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc
     1441 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac
     1501 gaaccccccg ttcagtccga ctgctgcgcc taatccggta actatcgtct tgagtccaac
     1561 ccggaaagac acgacaaagc gccactggca gcagccactg gtaactgatt tagggagtta
     1621 gtcttgaagt tatacgccag ttaaagctaa actaaacgaa cagttttggt gactgcgctc
     1681 cctttaagcc agttacctcg gttcatagag cgccaactct actgaacctt tcgaaaaaac
     1741 cgcccgccaa ggcggttttt ttcgttcaca gccggaagct tacggctgct gccagataat
     1801 ctcaagaaga aagtaaaaaa aatcgattaa aacacaagca atcaaagtta atctttgaca
     1861 taaagtctag ctaatcacaa tgaaagctgt acattttatt aaggtactaa caaatcgtat
     1921 ggcatataac aaaaacacga acagaggcta tcatgataaa ggaaataagt ttaaagaact
     1981 tcaaatgctt caatgagcta tatctaaagc aattaaagac tttaaatatc atagctggga
     2041 aaaacaacta cgggaaaaca tctatactgg atgctatttt ttgtttttat gatgtaaaaa
     2101 acccggctgt tttgttaaac attcaagcat tcagaaaaga aatggcagaa ataaacaaaa
     2161 acaagccatt ttgggttagt tactttcatg atatggacac atcacaaaag atgtccatag
     2221 tcattcgaga tgaacgctct gaagtaacac aaacatatga aacagaaaca aatcagagat
     2281 tagaatcaag tttgtctcta aacgttgaca ccgtactatc taaccaaaac atacctcgtc
     2341 aaactatcgg ttctcaggcc acagtaaact ctttaaaaat aaatgtgacc gagacaccca
     2401 atgcgaaaaa cagggtttcc aataagcttt tctctatgca attgaaagaa gatggcgtag
     2461 aaataaaatc cgaaataaaa aacgaaaggg atgggattaa ttataaattt aagacagcaa
     2521 caataataac aacatccaga aagataaaca aagaggcaac aattacaaat gtatctagcc
     2581 tactaacaca aaaaaggaaa aaagacatac taaaaaactt aaaaaaaata gatgacagaa
     2641 ttgtagatat agcaatatct gcgataggta acaataagga aatatattta gacattggat
     2701 ttagtgagct aaatgaaata tcaatgcttg gcgagggtat cagtcgagca ttatctttta
     2761 tcagctcagt gctggtacag gaaaactcaa ttattcttat tgatgaaatc gagaacggaa
     2821 tacattactc agtaattaaa gacataataa aatcattaat ttcatctgca aaacaaaaca
     2881 acaatcaaat atttgcaaca actcatagtc aagatgtgat acgagcaata aacgaaatag
     2941 atagcaaaaa tgaagatatc gcttatatta gactaggaag agaaaagaac tccctaaaac
     3001 caacagccgt acaattcaat atggatgatt tctcctactc agttgagaac gactgggagg
     3061 taagataatg aaagacataa ataatagcaa aaagatcatt ttcgttgagg gtgatgatga
     3121 ttttcatttt ctatgcaacc tacttgaaat acaaggcatt accgatgttt ttgtcgaaaa
     3181 aataaatgga aaatcaaaat taaaacaagc acttagagca ttcaaaaaca ttgattcttt
     3241 cgatgaaaaa gaaagtattt ttattatatt agatgctgac acatcatttt ctgacacaga
     3301 aagatcaata attgccacat tgcgagaact acaaatctcc tcccccagct cgcataacga
     3361 gatagctatg aatggcaata caaaaatttc tttctttata atgccaggaa aagacaagca
     3421 aggtgccatt gaagatctga taattgaaca cgcaagcact cgagcgttat atgaatacat
     3481 aatagaattt ttcgaaaaaa ttaaaaataa taaagatgac atctcagcga gtgacaaaga
     3541 gtacacttat ccaacaaacg aaaaaaaagc taaggtacaa gtctatctta gctgcaatag
     3601 agaatccgac tcaagaatag gaatatccat gaaaaatgga ataataaaca cagcagacaa
     3661 ttgctttgat gaaataaaag attttatttc aaaaatataa atccaaatca agccagccct
     3721 tcacgggctg gcttatcacc agggtgttat taaacgccgg ataacgtata gaaagcatca
     3781 tacactttag ttatcagcaa tctcaaacgt cctaaagaca tcaccagatg ccaaaacaat
     3841 gattgtgcag atctaatgta aaagacccag cacaagatgc tcccttaagt tatattctct
     3901 ctattcactt aaaacaattt aacataaaat cataattgta cgcaccgcta aaacggactc
     3961 aggacgtttc tgacgggaaa ccggtaaaac aggcaacaaa ccacccgaaa acccgccaga
     4021 aacgcgcagg ctctgttttt acggcatgca tgagtatgcg cgcaagtgca tagcgcggaa
     4081 attcacacca ttcagccagg ggaagtcctg tttccggcgt tcttctcctc cgaaaacggt
     4141 gaacggggac gcaatttatt gcgtggc
//
