LOCUS       NZ_CP075855             7684 bp    DNA     circular CON 21-JUL-2023
DEFINITION  Escherichia coli strain S19-651 plasmid pS19_651-c, complete
            sequence.
ACCESSION   NZ_CP075855
VERSION     NZ_CP075855.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993361
            Assembly: GCF_016404165.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 7684)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiniSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/21/2023 04:43:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,346
            CDSs (total)                      :: 5,228
            Genes (coding)                    :: 4,987
            CDSs (with protein)               :: 4,987
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 99 of 241
            Pseudo Genes (incomplete)         :: 130 of 241
            Pseudo Genes (internal stop)      :: 53 of 241
            Pseudo Genes (multiple problems)  :: 41 of 241
            Pseudo Genes (short protein)      :: 4 of 241
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075855.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S19-651"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pS19_651-c"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2019"
                     /collected_by="ILS/NENT"
     gene            481..675
                     /locus_tag="JFY28_RS26400"
                     /old_locus_tag="JFY28_026400"
     CDS             481..675
                     /locus_tag="JFY28_RS26400"
                     /old_locus_tag="JFY28_026400"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="WP_001367294.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAKG
                     ENIGWCIARAEYCKKTGRF"
                     /name="ANR family transcriptional regulator"
     gene            complement(2907..3329)
                     /locus_tag="JFY28_RS26405"
                     /old_locus_tag="JFY28_026405"
     CDS             complement(2907..3329)
                     /locus_tag="JFY28_RS26405"
                     /old_locus_tag="JFY28_026405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_032330703.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKSDTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3451..3729
                     /locus_tag="JFY28_RS26410"
                     /old_locus_tag="JFY28_026410"
     CDS             3451..3729
                     /locus_tag="JFY28_RS26410"
                     /old_locus_tag="JFY28_026410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032330704.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQINELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(3721..4011)
                     /locus_tag="JFY28_RS26415"
                     /old_locus_tag="JFY28_026415"
     CDS             complement(3721..4011)
                     /locus_tag="JFY28_RS26415"
                     /old_locus_tag="JFY28_026415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032330705.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEYDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            4252..5898
                     /gene="cka"
                     /locus_tag="JFY28_RS26420"
                     /old_locus_tag="JFY28_026420"
     CDS             4252..5898
                     /gene="cka"
                     /locus_tag="JFY28_RS26420"
                     /old_locus_tag="JFY28_026420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_165371048.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVVEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGTDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELNKLNELLGI"
                     /name="cka"
     gene            complement(5895..6185)
                     /gene="cki"
                     /locus_tag="JFY28_RS26425"
                     /old_locus_tag="JFY28_026425"
     CDS             complement(5895..6185)
                     /gene="cki"
                     /locus_tag="JFY28_RS26425"
                     /old_locus_tag="JFY28_026425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_072149383.1"
                     /translation="MYLKYYLHNLPESLIPWILILIFNDNDNTPLLFVFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            6209..6340
                     /locus_tag="JFY28_RS26430"
                     /old_locus_tag="JFY28_026430"
     CDS             6209..6340
                     /locus_tag="JFY28_RS26430"
                     /old_locus_tag="JFY28_026430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001368419.1"
                     /translation="MKIVIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6539..6682)
                     /locus_tag="JFY28_RS26435"
                     /old_locus_tag="JFY28_026435"
     CDS             complement(6539..6682)
                     /locus_tag="JFY28_RS26435"
                     /old_locus_tag="JFY28_026435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(7347..7544)
                     /locus_tag="JFY28_RS26440"
                     /old_locus_tag="JFY28_026440"
     CDS             complement(7347..7544)
                     /locus_tag="JFY28_RS26440"
                     /old_locus_tag="JFY28_026440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_028132815.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQTHFEAQYGIEQP"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          7179..7266
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="1.5000000000000001e-30"
     Typing          complement(6611..7168)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.405"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgctgaata tgtgcgcgat tttctgtctg cgttgtgtgc agttgttgtc ataaacacga
       61 atgttccggc tgttcctgat gtgcgtcact ttgacgtttt gacaaagtga cgcacgggag
      121 gtgcttgcgc aaacccgcga ataacggcgt ttaccgcgtg attttttaac atctgacaaa
      181 gtgacccggc gtgatggcca gttctggcca gagacgttgg cggggtgtcg gggcgcagcc
      241 atgactaggc ggcatttgtc tgattgcgtg tgcgcggtcc gacaaataca catcctgtcc
      301 cgtttttcgc acggttttta ttgattaccg gacagatgaa cccggattgc catgttgcga
      361 aaatcacgtt aaaacgatta cgagcgtgct gttgggctga tatatcaaca gtcatgccaa
      421 tatgtattaa cccgatgggc tcgctctttt ttaattcaaa taaaaatata aattacgaat
      481 atgaatcagg acaaagcaat ttatttagaa aataaatatt tttcacccgc aaacgaagct
      541 gtcagactgg agcgtgaagg tgattacatt ggagcatgga aaaaatggaa tgatgcgctt
      601 cattatgcca aaggagaaaa tataggttgg tgtatagcca gagctgaata ctgtaaaaag
      661 acaggccgct tttaattctg gcggggtttc aaaggggcgt ccactttggc gcgtcgacgt
      721 atcaaaatgc gccagcattt tgtatacagt cggtcacgtg cctccagaat ttaccctcaa
      781 aacgcccctg caactgatac acaacaaaag tataactaat atgtagttat tatataacta
      841 atgcataata aacagctcac caacggatcg catgtagtag acgctacata aaaaatcctg
      901 aaatggagga tttgtcataa agagattcct gtttttttag cgttctgatg caggacaggg
      961 caaacaccgc aaaaatgatg aaaaactgat taaccatatt gtctgtattc tgaatgaatt
     1021 acgaagcata gcgataaatt aaaaaatccc cctggattac agggggaagc catcagagtt
     1081 tgagtaatga gaaatattat tttggagaga gcaccacaaa tgcgctcttt tccaatctgc
     1141 cgtaaccatc ggcaaaagac aatcaggaca ggcgaaaaaa tccccctata tggcaagggg
     1201 gtgtcaatga ggacatggca attgtgagtt ttgtgtgtgt tttatgtgtt gcagtttgca
     1261 acgtcagaaa tttgccgtag ttcgtcatga gagacaacaa aaaaatcccc cgtgactggt
     1321 ggaggccagg gcaagggggg tagcaaatgc ctgaataatt ttcgtagttt tgatataccc
     1381 atcgttgcaa ccgcaacggg atgaatttgc cgcagttgtg cgcgaaaaac aatcctgatt
     1441 ccgggacagg atgtgtaatc gtataaccgc gcacgcacgg ctatgcgatt accacctggt
     1501 cagggctttg ccccgacagc ctcgacaccg ttcccgcgat acgatagaac atcagggcgc
     1561 gtttccgggt aaacgcatgt taatgaccgg gaattcattg atcagggacc ggagacgcgc
     1621 tacaaaactc ctgcgcgttt ctggcatcaa ctggccatgg ggatggtttg gcagtagcag
     1681 caagcgccag aaacgcgctc aaaacgtctc taacagcctg aacccaccaa aacataatga
     1741 aacattgcca gatgcaaaaa agtgcgttag aaagcgtctg agcgcgtttc cttgccacct
     1801 gaacgtcatc tctgggattc gagctcactc catcaaataa caaataaact tcaaagtctg
     1861 ataatgggtt ccccctgtaa tccaggggat ttttttcgct cactcattac cgcacaactc
     1921 gacatcacgc cggagacacg ctaaaacacc ctgcgcgttt ctggtgcaaa gagtagttcg
     1981 tagggatgat tacagtcagg tatgtaccag aatcgcgccc tgaacacggc tgaacgcctc
     2041 aaagcgaaac cattgcctca tgtaaaaaat cgcgttacaa agcctctgag cgcgtttctg
     2101 gcgtcttttt tgttgctgtg tcgctatact cgttgtcaga ttcgcgctca atcatcactg
     2161 attaatctgc gcttatcctt cgaccgatca cgctggtatt ttttgacaac cggcgcccat
     2221 gaaaacgcca gacgggaacc gtcatccgca ccctctgaca tgctggggat taaaaatccc
     2281 tgtgccgcac agccggcgtt gcataaaatt ccgctctgcg atgagtattg tttaatcaaa
     2341 aatccccgca gtttctccag aaaaacgcga gtctgttctc atgccccgga tgcccacccc
     2401 ccacgcaaaa ccaacttttt tctgatttgc tttttaaatc atgttgttat ggtttgaaaa
     2461 atatattttc ttattcagat ttatacaaaa gcgagttgtc ggcgactact ggatgattcg
     2521 gcaacgagtc gtcggtgatt tatcgatgac tcaacacact gtctctctgg tgttcagaaa
     2581 aagcgagtcg ccagagagtc gcgaggaaaa atactggatg actcgctgat gactcccggg
     2641 gtgaatcgct cacgactcgt ctgtaactac tgtctgattt cttctcatca gggctgaaaa
     2701 cgtccgaaaa tgcgccttat gggcatcagg cgcgcccaaa ccgatcgcca ttcagtttca
     2761 tgcatagtca tgcagtgcca tgaaaacaat cctgacgtat ttttccggtt tacccggggg
     2821 aaaacatctc tttttgcggt ttttgcgcca gaatcgcgct gagcgcgttt cagcggtgcg
     2881 tacaataagg gattatggta aatcccttac tgtctgccct cgtatccatc gagataaacc
     2941 gcagaaatgg tgtctgccat tagtttcatt tttttcggcg aaggcatatc gctgtttgtg
     3001 tattttctgc cagcaaaggt tgttgtgctc cggagtgagt gaaaaaattt ttctgagtgg
     3061 aattctttta cacgttgctg tgccgtttct gctgatactc cggctgcttt gtctttcatc
     3121 cccatatccc ggaactgctg aaagaaaggg agcatcatac gtatggtgtc tgatttctgg
     3181 gtgtagaagt taggatcaaa tccatcatct gaagcataaa cataatgccg ggtatgtcgt
     3241 tccaggctgg tctttggtgt gcatcctgta agagctattg ttgctgttat cagaggtatc
     3301 agaagcagcc tggcagcggc agatctcata aacatctcct tgcgttttta atcttgatac
     3361 tcagggagtt atatctccga agagtatctc ttatcatacg tcggtttact cccaatgtga
     3421 tttgatggtc aatgagtcag gaataatccc gtgaaaaaat gtgtgccagt cctgcttttt
     3481 agtgttcttt ctgcttcggt tgccagtcat gcggacagca atgtttttca acaaaaggcc
     3541 gagaagacaa aaaacggcat tgttgcggat gttaatgcca gacagaaaat gttcagtaac
     3601 agactgaaga tacaacttca gatcaatgag ttgagagcag agctggctgg caccagagat
     3661 gcccaaaaaa gagaggattt gcgtaaaaaa ctggagtctc tggagcggca aaagaaatcc
     3721 ttacgctgat gggggcgtat tttccccggt ttcttccata acctttttac cgttcagata
     3781 tttacgggcg aggcgaatgc gtcttgcctg gtataccgct gagtaagcct gggtgggatt
     3841 cacaccatta ctgatttcac tgaccacatg acgctgatca gccagtgtca gcgtgtaaac
     3901 ctgtttctgg atgaggtcac gggctttgtc atactcatat ccggcagcaa caagggtgtc
     3961 gagaagtcgt ttgtctgttt tgttcagttt atggcggggt gatttgctca catcgttctc
     4021 cgttttttca tgatccaaca gttttatatc tcatcctgat ttgctccggt gttctgtttc
     4081 gacgttctca atgcctgcct ccccatagtg tgatccttat tgcaaaaatg aaaatccatg
     4141 ctcttgacac ggacaatgct gagtagtagg ttttcactgt acataaaacc agtggttata
     4201 tatacagtat attgttttta atttattgtt ttaattatca aagaggaatt tatggctaaa
     4261 gaactaagtg gatatggccc aactgctggt gagtctatgg ggggaacagg ggcgaatctg
     4321 aatcagcaag gtggaaataa taatagtaat agtggggttc actggggtgg tggctctggg
     4381 catggtaata atggtggtca gggaaattca aattcatctg gttctacatc aactgtgatg
     4441 aaaaccggtg agtcatatct gactccgtgg ggtgatgttg tcattaataa tgatggcctt
     4501 ccggtgatga atggtattgt tatgacagaa gagaattcaa ctctggttga taatccattt
     4561 ggaggggtct ccagagttct taattcttta attagtgata tgccatcact ttttgcagaa
     4621 agctcaggaa ataacaacaa taacactgct tcagttaata ctgcaccaac gaatgctcag
     4681 gtcagtgata tggataagag ttcgaaggtg gtgggtaatg taattaatga aaaacaaaag
     4741 cagaagaata aaattgctac acagattagt gaaaaacaga agaaaatcga ggagatgaaa
     4801 aaagtattca agcatcattc ttatcatggt atcactgatc ttgagaggga tgttgatgag
     4861 ttgcaaaaaa aatcaaatca gcttgatgca gatattagca aacttaattc ttataaaaat
     4921 acattgcaaa gtaaaattgg tgatgtgaat aaacagaagg aagctgaaga aaaggctcgt
     4981 gaaaatgcag aggtcgctga acatgaaact cttaatgaag agaaacaggc ggtggtggaa
     5041 gcagagaagc gtttagctga ggcgaaagct gaactggcga aggctgaaag tgatgtacag
     5101 agtaagcaag ctactgtttc cagagttgca ggagagcttg aaaacgctca gaagagtgtt
     5161 gacgtgaagg tcaccggatt tccgggatgg cgtgacgttc agaaaaaact acagcgtcag
     5221 cttgaagcta agcaggctga gtactccgca gttgaaaatg aacttaagaa tgctgttagt
     5281 ttcagggatg gtaaggccgc tgaggttaaa gaggcagagc agaaattaaa agaagctcag
     5341 gatgctcttg aaaagagcca gataaaagat gctgttgata ctatggttgg attttatcaa
     5401 tatataaccg aacaatatgg agaaaaatac gcaaaaattg ctcaggattt agcggaaaag
     5461 tctaaaggaa agaaaattca aggtgttgat gaagctctgg cagcatttga aaagtataaa
     5521 aatgtactgg ataagaaatt cagtaaggtt gatcgggatg ctatttttaa tgcgttagag
     5581 tctgttaatt atgatgagtt atctaagaat ttaacgaaga tatcaaagtc gcttaaaatc
     5641 actagtcgag tatcttttct atatgatgtt ggtactgatt ttaaaaatgc aatagaaact
     5701 ggtaactggc gacctttgtt tgtcacgctg gagaaaagtg cggtagatgt aggtgtggca
     5761 aaaattgtgg cattaatgtt tagttttatt gttggtgtgc ctcttggttt ctgggggatt
     5821 gcaattgtca caggtattgt ttcttcttac ataggggatg atgagttgaa caaacttaat
     5881 gagttgttag ggatttaatt tctctttaga gtgtaatata tgaaacttgg caatcctact
     5941 gggattgcca ttaataaata aaacaatgca gataacttgc ataagtacca gggctctttt
     6001 tttaactttg tattttcttt gatatatcta gatatggtta atttagagta tggatatagc
     6061 aatacatgta ttgatgatat aaagacaaat aacaaaggag tgttatcatt gtcgttaaat
     6121 attaaaataa gaatccatgg tataagtgat tcaggtaaat tatgtaggta gtattttaga
     6181 tacataattt ttcttttgtt ggaggtgtat gaaaatagta atttttctaa ttacaatatt
     6241 attcttgtca ggatgtcaag caaactatat tcgtgatgta caaggaggaa ccgttgcacc
     6301 atcttcgtct tctgaactga cggggatcgc ggttcagtag aaaagatcaa aggatcttct
     6361 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctgcca
     6421 acggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttt
     6481 agtggagcac agataccaaa tgctgtcctt ccagtgtagc cgctataagg cttcaacttc
     6541 aagagttctg cgtatctaaa tccactatcc agttaccaat ggctgctgcc agtggcgtta
     6601 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6721 gataccaaca gcgtgagcta tgagaaagcg ccacgtttcc cgaagggaga aaggcggaca
     6781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccggggggaa
     6841 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagtggcgcg gccttttccg
     6961 ggttctcgtt ttttgcgcac atgtttcttt cggcgttatc ccctgattct gtggataacg
     7021 gtatcaccgt ctttgagtga ggtgactccg ctcgccgcag ccgaacgacc gagcgtagcg
     7081 agtcagtgag cgaggaagcg gaagtgtacc ggaagctgca ttttctcctt acgcatctat
     7141 gcggcatttc acacccggca tggtgcactt ttcatacaat ccgcactgat gccacatgat
     7201 taagtcagta tacactccgc taccgctacg tgactgggtc atggctgcgc cccgacgccc
     7261 gctaaagccc cctgacgtgc cctgacgggc tttgtctgct ggcgaaaaca ctcacggaca
     7321 agccgcgacc actttcgcca gcgcctttat ggttgttcta tgccgtactg agcttcaaag
     7381 tgagtctgta tgctgttctg gagttcttcc gccagttcgt gcagtcgttc gcacatggcg
     7441 gcctgttcgt ctgcatccag tgcatccagt ttttcgagca acgtcaggct ctggcttttg
     7501 ataaatcgcg ccatgttgag cgcggtctgc tgctgtttgt tcacgttctc ccgtctcctt
     7561 tactggtgtt tacgtccggc agtgcgtcac ctggtgtgat gacccggtga cgtcactgaa
     7621 aattatcaca caagcccagt cgcgcgtgtg ctgcgcgaca ttaacacccc gtgacgctta
     7681 tttt
//
