LOCUS       NZ_CP075743             9022 bp    DNA     circular CON 16-AUG-2023
DEFINITION  Escherichia coli strain CPF6 plasmid pCPF6-unnamed2, complete
            sequence.
ACCESSION   NZ_CP075743
VERSION     NZ_CP075743.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19296497
            Assembly: GCF_024499155.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Khine,N.O.
  TITLE     Colistin resistant E. coli isolates in pigs and pig farm environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9022)
  AUTHORS   Khine,N.O.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2021) Veterinary Microbiology, Chulalongkorn
            University, 39, Henry Dunant Road, Faculty of Veterinary Science,
            Bangkok, Bangkok 10400, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. galaxy verion 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/16/2023 05:13:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,189
            CDSs (total)                      :: 5,067
            Genes (coding)                    :: 4,851
            CDSs (with protein)               :: 4,851
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 66 of 216
            Pseudo Genes (incomplete)         :: 158 of 216
            Pseudo Genes (internal stop)      :: 32 of 216
            Pseudo Genes (multiple problems)  :: 42 of 216
            Pseudo Genes (short protein)      :: 4 of 216
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075743.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CPF6"
                     /isolation_source="Pig feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="pCPF6-unnamed2"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2018-09-05"
                     /note="mcr-3"
     gene            join(8852..9022,1..1401)
                     /locus_tag="KKZ13_RS25720"
                     /old_locus_tag="KKZ13_25530"
     CDS             join(8852..9022,1..1401)
                     /locus_tag="KKZ13_RS25720"
                     /old_locus_tag="KKZ13_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017782005.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_039026336.1"
                     /translation="MTHIACHINTLQLPFVACTVPLTFTLPAAGCTLIGRNGIGKSLLL
                     ELLAGQRRADSGKIEWLTPISWLSQFAPAQDHDCAASVADLIGWGEPLRALERIEQGSC
                     AQDDFELLADRWNLRSDLADLLAEAGLRLDPHVPVSTLSGGQLTRLRLLALFARPAHFL
                     LLDEPDNHLDASGIDWLSQQLARHRGGYLLVSHDRRLLNQTSHILELSEQGLQSARLSF
                     DEFLAQQAEVQAARSTSLAQAVRSEKAAARSLQKVREQAARRASQGRRERGSQAKILLD
                     FKAEQAGKSLGRVLDRHERGHSAQVEARTEAAAALAAVQPLIMQWPEEIERKGAAQPLV
                     ITAASHVRWHGQRVDLVLQRGERCLLGGRNGCGKSSLLQMLRGVLEPETGSFRINGKMA
                     CLDQGLSLIARDLSPLANLLTVAPTLAESDARTRLAGIALRGDRALTPCHGLSGGERLK
                     LGLLMVLAQLPDLLLLDEPDNHLDHPSRQLLTQVLADYPGTLLLVSHDPDFVAGVGVGK
                     ELNLSE"
                     /name="ATP-binding cassette domain-containing protein"
     gene            <1486..1776
                     /locus_tag="KKZ13_RS25725"
                     /old_locus_tag="KKZ13_25535"
                     /pseudo=""
     CDS             <1486..1776
                     /locus_tag="KKZ13_RS25725"
                     /old_locus_tag="KKZ13_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004661993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /name="type II restriction endonuclease"
     gene            complement(2592..2783)
                     /locus_tag="KKZ13_RS25730"
                     /old_locus_tag="KKZ13_25540"
     CDS             complement(2592..2783)
                     /locus_tag="KKZ13_RS25730"
                     /old_locus_tag="KKZ13_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3203..3316
                     /locus_tag="KKZ13_RS25735"
                     /old_locus_tag="KKZ13_25545"
     CDS             3203..3316
                     /locus_tag="KKZ13_RS25735"
                     /old_locus_tag="KKZ13_25545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058670733.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERHDKAPLAAATGN"
                     /name="hypothetical protein"
     gene            3761..4186
                     /locus_tag="KKZ13_RS25740"
                     /old_locus_tag="KKZ13_25550"
     CDS             3761..4186
                     /locus_tag="KKZ13_RS25740"
                     /old_locus_tag="KKZ13_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676030.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_004884531.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIIPVLLRVWSTELALTNQRVIAKVGFIRRNTVELRIDKVESLGIHQGILGRIFNYGSI
                     VIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMNSKQQ"
                     /name="PH domain-containing protein"
     gene            5251..5445
                     /locus_tag="KKZ13_RS25745"
                     /old_locus_tag="KKZ13_25555"
     CDS             5251..5445
                     /locus_tag="KKZ13_RS25745"
                     /old_locus_tag="KKZ13_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_072644309.1"
                     /translation="MNKQQQIALNMARFIKSQSLTLLEKLDALDADEQATMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6435..7109)
                     /locus_tag="KKZ13_RS25750"
                     /old_locus_tag="KKZ13_25560"
     CDS             complement(6435..7109)
                     /locus_tag="KKZ13_RS25750"
                     /old_locus_tag="KKZ13_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_072644308.1"
                     /translation="MRTIVYVDGFNFYHGLVKRSGYKWLDLYKLFEEQLLPIRSRESQL
                     IQVKFFTAEIKAKFASRGELAHQAQQAYHRALVQIYGDNLQIIKGYYSERQECAMTFNE
                     GHPPSKTDRTTVWKLEEKQTDVRMALEMYRDAIRGEAEQLVLCTNDSDMIPPIEMIKAD
                     RPELKIGIVVPREQDTSRVISSLASLGDWSLPYVSYEALRLALLPDKVPTRRKPILKPN
                     HW"
                     /name="NYN domain-containing protein"
     gene            7457..7873
                     /locus_tag="KKZ13_RS25755"
                     /old_locus_tag="KKZ13_25565"
     CDS             7457..7873
                     /locus_tag="KKZ13_RS25755"
                     /old_locus_tag="KKZ13_25565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283459.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_057103209.1"
                     /translation="MSDQKNNLDLQKQTLESKWGKEALSMGWSAIPTSLFFLQGALNMT
                     PTTFNVLLHLIIHWWKPYDWPHPSQESIAVRMGVSSRTVQRSLQELENYGLLTKIRTSK
                     DNPKYKGRNIYDMSLLVKSLNILTPDLNAKLNEK"
                     /name="helix-turn-helix domain-containing protein"
     gene            8038..8301
                     /locus_tag="KKZ13_RS25760"
                     /old_locus_tag="KKZ13_25570"
     CDS             8038..8301
                     /locus_tag="KKZ13_RS25760"
                     /old_locus_tag="KKZ13_25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_072644307.1"
                     /translation="MAVYTSETKKFLDSVSHDKTWWTSRYNPADDVPVSGIYRCTVCGK
                     EITSNKDDPFPPQNHHQHSQSQAIKWQLIVRTDTQGNGCGSK"
                     /name="protein L"
     Typing          5146..5230
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.393"
                     /coverage="87.0"
                     /evalue="5.15e-16"
     Typing          complement(5522..5603)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.857"
                     /coverage="98.0"
                     /evalue="1.43e-25"
     Typing          2963..3320
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.1"
                     /coverage="89.0"
                     /evalue="2.93e-164"
     Typing          5904..6427
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.604"
                     /coverage="99.0"
                     /evalue="7.85e-161"
ORIGIN
        1 aagatcgagt ggttgacccc catcagctgg ttgtcccagt ttgcaccggc tcaagatcat
       61 gactgcgcag ccagtgtggc cgaccttatc ggctggggcg agccgctacg ggcccttgag
      121 cgcatcgagc aggggagctg cgcccaggac gatttcgagt tgctggctga tcgctggaac
      181 ctgcgcagcg atcttgccga cctgctggca gaagcaggcc tcagactcga tccccatgtc
      241 ccggtcagca ccctgagcgg tggccagctc acccgtttgc gcctgctggc gctgtttgcg
      301 aggcctgctc actttctgtt gcttgatgag ccagacaacc acctcgatgc ctctggcatc
      361 gactggctaa gccagcaact ggccagacat cgcggaggtt acctgctggt gagccacgac
      421 cggcgcctgc tcaatcaaac cagtcatatt ctggagctga gcgagcaggg gttgcagagt
      481 gcccgcctct cgtttgacga gtttctggca cagcaggccg aggtacaggc tgcccgcagc
      541 accagtttgg cgcaagcggt gcgaagcgaa aaggcggcgg cgcgcagctt gcaaaaagtg
      601 cgtgaacagg cggccagacg agccagtcag gggcggcgtg agcgcggctc ccaagccaaa
      661 atcctgcttg atttcaaggc ggaacaggca ggcaaatccc ttggcagagt gctggaccgc
      721 cacgagcggg gccacagtgc gcaagtggag gcccgcaccg aggcagcagc cgcgctggcg
      781 gcggtgcagc cgctcatcat gcagtggccg gaggagatcg agcgcaaggg ggctgcccag
      841 ccgctggtga ttacggctgc gtctcacgtg cgctggcatg gccagcgggt tgatctggtg
      901 ctgcaacggg gtgagcgctg tttgctcggc ggtcgcaatg gctgcggcaa atcgagtctg
      961 ctgcagatgt tgcgtggggt gctggagccc gagacgggca gctttcgcat caatggcaag
     1021 atggcttgcc tcgatcaggg gctcagcctg attgcgcggg atctcagccc gcttgccaac
     1081 ctgttgacag tggctccgac actggccgag agtgatgcga gaacacggct ggcgggcata
     1141 gccctgcgcg gcgatcgtgc tctgacgcct tgtcatggtc tgagtggggg ggagaggtta
     1201 aaactgggtc tgctgatggt actggcgcaa ctgcccgacc tgctgctgct cgatgaaccg
     1261 gacaaccatc tggatcaccc ctcgcgtcag ttgctcactc aggtcctggc ggactaccca
     1321 ggcactctgc tattggtttc tcacgatccc gacttcgttg caggggttgg ggttggtaaa
     1381 gaattaaatc tttcagagtg accgcaagat gattgaccta gatgcccttg tggcaggggg
     1441 ttagaggagt tgacggaaat ttcggcggtt ccggcttaca accccgaata ccataacccg
     1501 gcgttcgact cgctgaagct gaccatgctt ggtgtgaaat cgacctgcaa ggaccgctgg
     1561 cggcaggttc tcgccgaagc agaccgcatt gatgacaagc atctgctgac gctggaaaca
     1621 gcaatctcca cgcatcagac cgacgaaatg gcagcaaagc ggctgcaact cgttctgccg
     1681 cgcagtctgc accagaccta tacgccagcc cagcaggcat ggttgatgga tgttgtcagt
     1741 ttcacggaac tggtacgtgc tcgacagaat gcgtgagtgc tgaacttcgg cttagcgtac
     1801 gattttttcc gttttctgag ccgacccctt tctgaggcct ccggcctgaa acacacacct
     1861 ttctgtgccc atatcgagaa aaaacgcccc tgatgattct ggcgcgactg catggctatg
     1921 cacggacata cctggccggg attttagcgg ctaaaatccg atgaacgccc tgaaagccac
     1981 cacgaaccac cccgataacg gtgaacggat gcgcaattta ttgcgtggcc gtgaggcgtt
     2041 gccggcacag ccggaaaaac cattttcagc acatgcagga ggcccaagaa gagggggcgg
     2101 atgtacgaat agttcagagg tcagatctga cgtaaaaatg atgtaaaagt gacgtaaaag
     2161 tgatgtactt ttgatgtaat tctgttgtac acccgcgatc tgacgtaaaa gtgatgtaat
     2221 tttgatgtac ttttgatgta attctgttgt acacccgcgt tctggggtaa aagcagatgc
     2281 aattttgtag tttatgtgta gtcgctgaac ccatagggtc atacgagcat gaccctttcc
     2341 cggaaaagga cgctttttag ccagaatcat ccttaacctg cggcgccagc actcgggagt
     2401 ggtcgtttta tatactaaaa aatgacatac acctaacgca gctgcgattt tcatcgcaac
     2461 ttcatgaaaa ataaataaaa aagaaaaaac acagggattc tgggtattac cggggtaata
     2521 atgttaagcg ctaacacaat gaaaattaag gcgatgccgg tattactccg gtaataccca
     2581 gaacggagcc attacgcacc gtcctgctgc tcattcaggc gcgctgacag gcttcggtaa
     2641 aggcgttccg catcctcatg gagtttctcg cagaaatcag cctcatgatc cagatccagc
     2701 tcattaagcc tgtccagaag aagcagggac tgatcctgga tgaatttagc catgttaacg
     2761 gcagtctttt gttgtttgtt catgaaagcc ccggcgcgaa agtgcttact gtacgttcgg
     2821 atggttttcc gcttcctcgc tcattcgctc actccgttcg gtcatgagtc tgcggcgagc
     2881 ggagggagga taagcagata acgtaacggt aagcataacg aaagctaagg acaagggcga
     2941 aaaggccacg ccccgccggg gcgtttttcc ataggctccg cccccctgac aagcatcacg
     3001 aaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag atcccaggcg
     3061 tttccccctg gtagctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
     3121 attccgctgt tatggccgcg tttatctcat tccacgcctg acactcagtt ccgggtaggc
     3181 agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgact gctgcgcctt
     3241 gtccggtaac tatcatcttg agtccaaccc ggaaagacac gacaaagcgc cactggcagc
     3301 agccactggt aactgattta gaggagttag tcttgaagtt atgcgccggt taaggctaaa
     3361 ctgaaaggac agatttggtt actgcgctcc tccaagccag ttaccgcggt tcaaagagtt
     3421 ggtagctctg agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt ttccagagaa
     3481 agagattacg acgcaacgga caggatctca agaagatcat ctgtgatatt cgacagatat
     3541 gggataaagg attgcaacgc caagagtatg tgagacatag cagcagccac caccaccaca
     3601 gaatcaagcc agttgcgtca cgaaaggaaa gaaccgaact gtgcgacggt cgctacgctc
     3661 tccgtaaatt ttgtataccc accacggaga cataaagttt ctattgctga ttcgccttaa
     3721 aaagggaaaa atcctatttt ttctaaatgg atataacctt atgggaagct acgtagacca
     3781 atcattaacg agaaatgaat ccgtaatatc tcgcgctcaa acctcctgga ttccaacaat
     3841 catcccagta attatcggaa tcttgttgct gcctttttac ggcttagggt tacttataat
     3901 aatccctgtg ctccttagag tttggtcaac ggagcttgca ttaactaacc aaagagtcat
     3961 cgcaaaagtg ggttttatcc gacgcaatac ggtggaactc cgtattgata aagttgaaag
     4021 ccttggcata catcaaggga tactgggacg aatctttaac tatgggtcta tcgtgattaa
     4081 aggcactggc ggaaccaaca ctccaattcc aaacatcaag gcgccaatgc aattcagatc
     4141 gattgtgaac aatcatatcg aggaaatgaa ttctaagcag cagtagcttc ctcgtagcat
     4201 tacaaaaaaa gccctgcggg gctttttttg tacctgccaa tcagctggat ttaccataac
     4261 atccgttgcg tgcaccatct cagacgccct cagggcattt ctgcgccctc cagcctattt
     4321 ccggcatctt tccctgtcca tatcgtgaaa aaacgcgcct gatgatttct gcgcatctgc
     4381 atggctatgc acgaacatgc ctggccggaa ttttagcggc taaaatcctg cgaactgctc
     4441 aaaaggctcg ctgaacccca tcgataacgg tgaacgggtg cacagtttac tgtgcggtcg
     4501 tgaggcgttg agcaacgagc tttgcgaggc ggtaggggtt ctgagagcca gctgcaggct
     4561 caggagaaaa cattctgttt tcggggcaga taccgcaaat gacgtcattt tgcggcgtct
     4621 gccctatcct gcatcgcaac gtctaaagga tgtgagtaca aacgccagca ctttgcgaat
     4681 cctgccctcg atctgaacaa aagcaaaatt tcgtacgtac gaaattcagg gtatcaggct
     4741 gcgcctgatg aaatgttttt ggtgccggcg gtactccaaa aacacatctt gtccgacagt
     4801 tttgtggtgc cggcgaagcg gcggtggcca caaaacattt cctgtcccca tcgattcatt
     4861 cgagaaacat aagaggcgaa gaaggggtca gtttggagat cggaaatttt tgtacgttaa
     4921 gcgcattatt taaccaatct tggttcgtaa ggggttgatt ttgcgtcagt ggctatcgct
     4981 tttaagttgt cgctcttgca gtatcgcatt taggttatcc gattaaggtc aaacctctga
     5041 aaatgccgta tagcgcggga gtacacccta ttgctatagg taagtctgtt caaaaaacag
     5101 gcttaccgta caataattct ctatatccaa actgacccct aaagaaacag gacaggatgt
     5161 gcaattgtaa taccgtcgcc cgcaacgtct tacaattacc acctggtcag ggtccacccg
     5221 acacccggta ctgacggaga acaacggaag gtgaacaaac agcaacaaat tgcgctgaat
     5281 atggcgcgat ttatcaaaag ccagagcctg acactacttg aaaaactgga tgctctggat
     5341 gccgacgaac aggcgaccat gtgcgaacga ctgcacgaac tcgcggaaga gctccagaac
     5401 agcatccagg ttcgctttga gaccgaaagt gaaacaggga cataacgaag ctgacggagg
     5461 cgctcacggc ttgtccgcga gcgttttcgg cagcagacaa gcccgtcagg gcgcgtcagt
     5521 ggggttcggg gcgaagccct gaaccagtca cgtagagata gcggagtgta tacgggctta
     5581 atcatgcagc atcagtgcgg attgtatgaa aagtttgcca tatccggagt gaaatgccgc
     5641 acagatgcgt aaggagaata tgcctgttca ggcgctcttc cgcttcctcg ctcactgact
     5701 cgctacgctc ggtcgttcgg ctgcggcgag cggtgtctgc tcactcaaag atggtaatac
     5761 agttatccac agaatccgag gataacgccg gaaagaacat gtgagcaaca agacgaaacc
     5821 cggaaaaagg cagcgccggt ggcgtttttc cataggcttc gcccccctga cgagcatcac
     5881 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gacttaaaga taccaggcgt
     5941 ttccccctgg tggctccctc gtgcgctctc ctgttcctgc cattcggttt accggtgtca
     6001 ctccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
     6061 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgactg ctgcgccttt
     6121 tccggtaact atcgtcttga gtccaatccg gaaagacacg acttatcgcc actggcagca
     6181 gccattggta acaggattca cggagcagag tagtagaagt gcttctacag agttcttgaa
     6241 gtgatggcct gactgcggct acactggaag gacagttttg gtatctgcgc tccgtgaaag
     6301 ccagttacct tcggaaaaaa gagttggtag ctcttgattc cggcaaacaa accaccgttg
     6361 gtagcggtgg tttttttgtt tgcaggacat gagattacga cgagagtaaa aggatcacaa
     6421 gaagatccgg ctgtttacca gtgatttggc ttcagtattg gctttcgcct agtggggact
     6481 ttatctggca ataaagctaa tcttaaggct tcataggata catatggcaa gctccaatcc
     6541 cctaaactag ccagactact aatcactcga ctagtatctt gttcccttgg cactacaatt
     6601 cctattttta actcaggtcg atcggctttt atcatttcta ttggcggtat catatctgag
     6661 tcgttagtac agagaaccaa ttgttctgcc tcaccacgga tcgcatcgcg atacatttct
     6721 aaagccattc taacgtcggt ctgcttttct tctaacttcc agaccgttgt tctatccgtt
     6781 tttgatggcg gatgcccctc attaaaggtc attgcacatt cctgacgctc tgagtaataa
     6841 cctttgatta tctgtaggtt atcgccatag atctgtacta atgctcgatg gtaggcttgc
     6901 tgagcctggt gcgccaattc accccgagaa gcaaactttg ctttgatctc agcagtaaag
     6961 aacttcacct gaatcaactg tgactctctt gatcttatcg gaagtagttg ttcttcaaag
     7021 agtttataga gatctaacca cttatatccc gaacgcttaa ccaatccgtg ataaaaatta
     7081 aacccatcta cgtaaactat tgttctcatt ttatttcgcg cataaaaaaa ccgcctctaa
     7141 aggcggtttt aacaacaaaa actaacgtta gcaaacagtt taagttgtag ttcctcaggg
     7201 ttaaaactaa ccgagagggc ggtttcaagc tcctgaagga agacgcgtaa cttaatcgct
     7261 gcccacctaa agggaggttt agagcctcaa gggagctgcg taacttaatc gcgctaccaa
     7321 gaggtgagtg tagattaagt atagttaaat ataatgatat tataagcgcg atggtgtcgt
     7381 actaattata tgaattaatt taccaacctg ctaagctgaa gtcttctatg aagacttcag
     7441 cttagcaggt tggtatatgt cagatcaaaa aaataattta gaccttcaaa aacagacact
     7501 tgaatctaaa tgggggaagg aggcgttaag catgggatgg tcagccattc ctacatccct
     7561 tttcttcctg cagggcgcac tcaatatgac ccccacaaca ttcaatgtgc tacttcatct
     7621 aatcatccac tggtggaaac cctatgattg gccacatcca tctcaagaga gcatcgcggt
     7681 tagaatggga gtttcatcaa gaacagttca acgctcactc caagaacttg aaaattacgg
     7741 attgcttaca aaaattcgta caagcaagga caaccctaag tacaaagggc gaaacattta
     7801 cgatatgtct cttttagtta agtcactaaa cattcttacg ccagacctta atgccaaact
     7861 gaacgaaaag taaccataaa ataattttaa aaatcagacc attacagctt atatttacca
     7921 tagcatctct attttagcgt ttttcaatca tcgccagatg attgaaaaac gctaaaaaaa
     7981 accaaactta taaaaaggat ccccctccgc cctatacatt ttggaaagga aaggcaaatg
     8041 gctgtataca cctctgagac taaaaaattt ttagacagtg tttctcacga taaaacttgg
     8101 tggacatcac gatataaccc agctgacgat gtaccggtat cagggatcta tcgttgtact
     8161 gtttgcggaa aagagataac tagcaataaa gatgacccgt ttccgccgca aaaccatcat
     8221 caacacagcc aaagtcaggc aatcaagtgg caattaattg tcagaacaga tacgcaagga
     8281 aatggttgtg gttctaaata actcaaaagc ccttaggggc ttttttgtac ctagcggtca
     8341 gacggattaa acataacatc cgttgcccgc accgcctcag aggccctcag ggcgtttctg
     8401 gggacggtag agaaaacgga caaaatcgta cgctaagcat gcggcctgtc aggttgaggc
     8461 catagagttt gacgtttagg cgtcagataa tgcagaaagc ggccctagtc tgacgcgatg
     8521 ccagtgagcc gactgacgcc cagttgaggt atagcccaga ctgacacttg acttattgat
     8581 acaacatgca agtgagtgat accggaacat ttggcatttt ttactatctg aagtatgatt
     8641 ttactttcat cagtaaagag tatttcgccg atgcgcattt aacctgtccg caccccactt
     8701 ttgattcttc tcccgccagg gagagcgggt gcgtctcacc gtgacaggtt gatgctgatt
     8761 cctctgctgt tttcccacta gtggacacgt gtccctgtca tttggtttag ctcttggcct
     8821 gcggcttccg cattggccta caggagttca gatgacccat atcgcatgtc atatcaacac
     8881 tttgcagctg cccttcgtcg cctgtactgt gccccttacc ttcactttgc ccgctgcggg
     8941 ctgcaccctg atcggccgta acggcatcgg caagagcctg ctgctggaac tgctggcggg
     9001 ccagcgccgc gcggattctg gc
//
