LOCUS       NZ_CP075734             8153 bp    DNA     circular CON 16-AUG-2023
DEFINITION  Escherichia coli strain CP52E plasmid pCP52E-IncQ1, complete
            sequence.
ACCESSION   NZ_CP075734
VERSION     NZ_CP075734.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19296499
            Assembly: GCF_024499115.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Khine,N.O.
  TITLE     Colistin resistant E. coli isolates in pigs and pig farm environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8153)
  AUTHORS   Khine,N.O.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) Veterinary Microbiology, Chulalongkorn
            University, 39, Henry Dunant Road, Faculty of Veterinary Science,
            Bangkok, Bangkok 10400, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. galaxy verion 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/16/2023 04:22:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,843
            CDSs (total)                      :: 4,727
            Genes (coding)                    :: 4,479
            CDSs (with protein)               :: 4,479
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 248
            CDSs (without protein)            :: 248
            Pseudo Genes (ambiguous residues) :: 0 of 248
            Pseudo Genes (frameshifted)       :: 91 of 248
            Pseudo Genes (incomplete)         :: 149 of 248
            Pseudo Genes (internal stop)      :: 43 of 248
            Pseudo Genes (multiple problems)  :: 35 of 248
            Pseudo Genes (short protein)      :: 4 of 248
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075734.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CP52E"
                     /isolation_source="Anal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pCP52E-IncQ1"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2017-06-10"
                     /note="mcr-1"
     gene            1..840
                     /locus_tag="KKZ11_RS24040"
                     /old_locus_tag="KKZ11_23930"
     CDS             1..840
                     /locus_tag="KKZ11_RS24040"
                     /old_locus_tag="KKZ11_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_094337857.1"
                     /translation="MATHKPINILEAFTAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVLYLPAEDPPTAIHHRLHALGAYLNDEQRQAVAA
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /gene="repC"
                     /locus_tag="KKZ11_RS24045"
                     /old_locus_tag="KKZ11_23935"
     CDS             827..1678
                     /gene="repC"
                     /locus_tag="KKZ11_RS24045"
                     /old_locus_tag="KKZ11_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            1973..>2389
                     /locus_tag="KKZ11_RS24050"
                     /old_locus_tag="KKZ11_23940"
                     /pseudo=""
     CDS             1973..>2389
                     /locus_tag="KKZ11_RS24050"
                     /old_locus_tag="KKZ11_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /name="dihydropteroate synthase"
     gene            2747..3769
                     /locus_tag="KKZ11_RS24055"
                     /old_locus_tag="KKZ11_23945"
     CDS             2747..3769
                     /locus_tag="KKZ11_RS24055"
                     /old_locus_tag="KKZ11_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821153.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]; GO:0008324 - monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_000083351.1"
                     /translation="MSQSHAHDHGHDHDHDHTPTVTSANERKVLVSFFLIFGFMLVEAV
                     GGVLSGSLALLADAGHMLTDAVALALAYAAFRFGRRAADSKRTFGYLRFEVIAGFLNAV
                     TLFAIVAWIAYEAWERLQAPPVILAGPMMIVAVLGLLINVLVLWIMTRGETDHVNVKGA
                     ILHVMGDLLGSVGAIVAAIVIYFTGWTPIDPILSVLVAALILRSAWKLLAKSIHILLEG
                     APEDASPEKVEQGLMSTVPGLAAVSHVHVWQLTSGRTMATLHVRPNVDEEARAVVKRVE
                     VVLREQFSIEHATVGIDWNSEADENICSLQPTTDRQDHSGHGHSEHNHDHDHSAPGHKH
                     "
                     /name="cation diffusion facilitator family transporter"
     gene            3813..4190
                     /locus_tag="KKZ11_RS24060"
                     /old_locus_tag="KKZ11_23950"
     CDS             3813..4190
                     /locus_tag="KKZ11_RS24060"
                     /old_locus_tag="KKZ11_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821152.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000203935.1"
                     /translation="MTSRKTTSSAIAHCSTSSHGATTDVLALSQSDVAVLAETFRLLGD
                     QSRLKILLQCMRGSVAVGDIADSLDLSQSLVSHHLRLLRGARLVRGERQAKHIFYGIAD
                     QHVSQVLQDMAVHISEDRNDD"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(<4295..4456)
                     /locus_tag="KKZ11_RS24065"
                     /old_locus_tag="KKZ11_23955"
                     /pseudo=""
     CDS             complement(<4295..4456)
                     /locus_tag="KKZ11_RS24065"
                     /old_locus_tag="KKZ11_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(5056..5223)
                     /locus_tag="KKZ11_RS24070"
                     /old_locus_tag="KKZ11_23960"
     CDS             complement(5056..5223)
                     /locus_tag="KKZ11_RS24070"
                     /old_locus_tag="KKZ11_23960"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_161403145.1"
                     /translation="MTDLKLDDYTDADFIDELLSDELRCECGEELHYYDDGSLSRCNSC
                     DPDRETNPNL"
                     /name="hypothetical protein"
     gene            complement(5250..5534)
                     /locus_tag="KKZ11_RS24075"
                     /old_locus_tag="KKZ11_23965"
     CDS             complement(5250..5534)
                     /locus_tag="KKZ11_RS24075"
                     /old_locus_tag="KKZ11_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_094336938.1"
                     /translation="MAKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPA"
                     /name="mobilization protein"
     gene            5734..>6372
                     /locus_tag="KKZ11_RS24080"
                     /pseudo=""
     CDS             5734..>6372
                     /locus_tag="KKZ11_RS24080"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            6482..6895
                     /locus_tag="KKZ11_RS24085"
     CDS             6482..6895
                     /locus_tag="KKZ11_RS24085"
                     /inference="COORDINATES: protein motif:HMM:NF036929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_223384840.1"
                     /translation="MSAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRETQAAWAKLAKELREVASEVSDAAQSARSAARGWHWKLWLTVMAASM
                     MPTLVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            6940..7863
                     /locus_tag="KKZ11_RS24090"
     CDS             6940..7863
                     /locus_tag="KKZ11_RS24090"
                     /inference="COORDINATES: protein motif:HMM:NF028103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_198870443.1"
                     /translation="MGCERFDIGVRDATTGQMMNREWSASEVLQNTPWLKRMNAQGNDV
                     YIRPAEQERHGLVLVDDLSELDLEDMKAEGREPALIVETSPKNYQAWVKVADAAGGELR
                     GQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRTGYQPWVLLRESKGKTATAG
                     PALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRYGDD
                     LSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQ
                     LARAELAQAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            7919..8125
                     /gene="urfF"
                     /locus_tag="KKZ11_RS24095"
                     /old_locus_tag="KKZ11_23975"
     CDS             7919..8125
                     /gene="urfF"
                     /locus_tag="KKZ11_RS24095"
                     /old_locus_tag="KKZ11_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_094336940.1"
                     /translation="MKDHRDTQTGNLLATPNAIRQARFADAQRKLGRKARKIWATDDEA
                     EALRLYLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             1974..2488
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="63.1"
     Typing          5489..5645
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.671"
                     /coverage="100.0"
                     /evalue="1.08e-59"
     Typing          19..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.813"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          5735..7860
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.769"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttca cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgctctacc tgcccgccga agatccaccc
      241 accgccatcc atcaccggct tcacgccctg ggcgcatacc tcaacgacga gcagcggcaa
      301 gccgtggccg ctggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct tatgctattc
     1861 tgctcctgca gctaatggat caccgcaaac aggttactcg cctggggatt ccctttcgac
     1921 ccgagcatcc gtatgagact catgctcgat tattattatt atagaagccc ccatgaataa
     1981 atcgctcatc attttcggca tcgtcaacat aacctcggac agtttctccg atggaggccg
     2041 gtatctggcg ccagacgcag ccattgcgca ggcgcgtaag ctgatggccg agggggcaga
     2101 tgtgatcgac ctcggtccgg catccagcaa tcccgacgcc gcgcctgttt cgtccgacac
     2161 agaaatcgcg cgtatcgcgc cggtgctgga cgcgctcaag gcagatggca ttcccgtctc
     2221 gctcgacagt tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct
     2281 caatgatatt cgcggttttc cagacgctgc gttctatccg caattggcga aatcatctgc
     2341 caaactcgtc gttatgcatt cggtgcaaga cgggcaggca gatcggcgcg gtcatgggct
     2401 taccatcgac cttcattgtc atgtctctga tcggcttggc cggcgtggtg ctggcgctct
     2461 ggctgtggcc ggccagcgac ccttctgacg tggtgcatga ccaccccagc ttgccgccgg
     2521 atcaccctca tttacgcaca cacgcagacc gaggcaagca ccatcaccag ctgattgtgg
     2581 atgacctgca ccgtatctgg ccgaaaggat aggcacattt tctcgttgcg gcaccacaac
     2641 gtatggcttc ggcggctggg aagggtgagc gaaaatactt cacatgaaca tgtattcatg
     2701 tgttatattt tcaccatagt caaacactca accagcagag gcgcccatgt cccaatccca
     2761 tgcacatgac catggtcacg accatgatca cgaccacacc cccacagtga ccagcgccaa
     2821 cgagcgcaag gttctggttt ccttcttcct gatcttcggc ttcatgctcg tggaagctgt
     2881 cggcggcgtg ctgtcgggct cgctagccct acttgccgat gctggccaca tgctgaccga
     2941 cgccgtggca cttgccctgg cttacgctgc cttccgattc ggtcgccgcg cagccgacag
     3001 caaacgcaca ttcggttacc tgcgcttcga ggtcatcgcc ggcttcctga acgccgtgac
     3061 cctgttcgcg atcgtcgcat ggatcgccta cgaggcgtgg gagcggctgc aggcgccgcc
     3121 cgtcatcctc gccggcccga tgatgatcgt cgccgtgctc ggcttactga tcaacgtcct
     3181 cgtgctgtgg atcatgaccc gcggcgaaac cgatcacgtc aatgtgaaag gtgccattct
     3241 ccacgtgatg ggtgacctgc tgggctctgt cggcgccatc gttgcagcga tcgtcatcta
     3301 tttcaccggc tggacgccga tcgaccccat tctgtcggtc ttggtggcag cgttgattct
     3361 gcgcagcgcc tggaagctcc tggccaagtc gatccatatc ctgctggaag gcgcgccaga
     3421 ggatgcttcg ccggaaaagg tggaacaagg cctgatgagc actgtacccg gcctggcagc
     3481 tgtcagccac gttcatgtgt ggcaactcac ttccggtcgc acgatggcca cactgcacgt
     3541 tcgacccaat gtggacgagg aggcgcgagc cgtggtcaag cgcgtcgagg tggtgcttcg
     3601 agagcaattc agcatcgaac acgccacagt cggaatcgac tggaactcgg aggctgacga
     3661 gaacatctgc agcctgcaac ccacgacaga ccgtcaggac cacagcggcc atggccacag
     3721 tgagcacaat catgaccatg accactccgc tcctggtcac aagcattgag gccgtgcgag
     3781 ccggctcgca gttcacttga gggtttgaat ccatgacatc acgcaaaacc accagctccg
     3841 ctattgctca ctgttcgacc tccagccacg gcgcgaccac tgatgtcctc gcactgtcgc
     3901 aaagtgacgt cgctgttctg gccgagacct tccgcctgct gggcgaccaa tcgcgattga
     3961 aaatcctgtt gcagtgcatg cgcggatccg tcgcagtggg cgacattgcc gattcgctag
     4021 acctgtcgca atccttggtc agccatcacc tgcgcctgtt gcgcggcgcg cgcctcgtgc
     4081 gcggcgagcg ccaggccaag cacatcttct acggcatcgc cgaccagcac gtcagccagg
     4141 tactgcagga catggcggtt cacatttcgg aggacagaaa cgacgattga ctgaacgtgt
     4201 gagtgcgcgc cggcctactc cttcaagggg taggcttttt tttggctatc aatctagaga
     4261 aacaccgacg cgccttgctg aatgcctaga accccacata gcccggcaat tcctttccct
     4321 gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga tagagaaggg
     4381 tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg gccgtcctgg
     4441 ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca gtctatcgct
     4501 tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca ttgccagcca
     4561 gtgcccgtca ctcccgtact aactgtcacg aacccctgca ataactgtca cgcccccctg
     4621 caataactgt cacgaacccc tgcaataact gtcacgcccc caaacctgca aacccagcag
     4681 gggcgggggc tggcggggtg ttggaaaaat ccatccatga ttatctaaga ataatccact
     4741 aggcgcggtt atcagcgccc ttgtggggcg ctgctgccct tgcccaatat gcccggccag
     4801 aggccggata gctggtctat tcgctgcgct aggctacaca ccgccccacc gctgcgcggc
     4861 agggggaaag gcgggcaaag cccgctaaac cccacaccaa accccgcaga aatacgctgg
     4921 agcgctttta gccgctttag cggcctttcc ccctacccga agggtggggg cgcgtgtgca
     4981 gccccgcagg gcctgtctcg gtcgatcatt cagcatcggc atcatctgct aatttcccgc
     5041 aatgttcgca ggtaatcata ggtttggatt ggtttctcta tctggatcac acgaattgca
     5101 gcgactcaat gaaccatcat cgtaataatg aagctcctct ccgcattcac agcgcagttc
     5161 atcactgagc agttcatcaa taaaatcagc gtctgtgtaa tcgtctagtt ttaaatcagt
     5221 cattattgct ttcctttatt agtttaaatt caggccggct catccttctg gcgtggcggc
     5281 agaccgaaca aggcccggtc gtggtcgcgt tcaaggtacg catccattgc cgccatgagc
     5341 cgatcttctg gccactcgct gctgttcacc ttggccagaa tcatggcgcc caccagcacc
     5401 ttgcgccgcg tttcgttctt gcgctcttgc tgctgctccc ttgccctcac ccgctgaatc
     5461 tcggcattga tccgtgctcg ctgttcttcg agcttggcca gcctatccgc cgccttgttg
     5521 ctccccttag ccatcttgac acctcattgt taatgtgctg tctcttaggc tatcatggag
     5581 gcacgacggc ggcaaacccc gaccgtactt tgtacgggag ggcgcactta ccggtttctc
     5641 ttcgagaaac tggcctaacg gccacccttc gggcggtgcg ctctccgagg gccattgcat
     5701 ggagccgaaa agcaaaagca acagcgaggc agcatggcga tttatcacct tacggcgaaa
     5761 accggcagca ggtcgggcgg ccaatcggcc aaggccaagg ccgactacat ccagcgcgaa
     5821 ggcaagtatg cccgcgacat ggatgaagtg ctgcacgccg aatccgggca catgccggag
     5881 ttcgtcgagc ggcccgccga ctactgggat gctgccgacc tgcatgaacg cgccaatggg
     5941 cggctgttca aggaggtcga atttgccctg ccggtcgagc tgaccctcga ccagcagaag
     6001 gcgctggcgt ccgagttcgc ccagcacctg accggtgccg aacgtttgcc gtatacgctg
     6061 gccatccatg ccggtggcgg cgagaacccg cactgccacc tgatgatctc cgagcggatc
     6121 aatgacggca tcgagcggcc cgccgctcag tggttcaagc ggtacaacgg caaggccccg
     6181 gagaagggcg gagcgcagaa gaccgaagca ctcaagccca aggcatggct tgagcagacc
     6241 cgcgaggcat gggccgacca tgccaaccgg gcattagagc gggctggcca cgacgcccgc
     6301 attgaccacc gaacgcttga ggcgcagggc atcgagcgcc tgcccggtgt tcacctgggg
     6361 ccgaacgtgg tggagatgga aggccggggc attcgcaccg accgggcaga cgtggccctg
     6421 aacatcgaca ccgccaacgc ccagatcatc gacttgcagg aataccggga ggctatagac
     6481 catgagcgca atcgacagag tgaagaaatc cagaggaatc aacgagttag cggagcagat
     6541 cgagccgctg gcccagagca tggcgacact ggccgacgag gcccggcagg tcatgagcca
     6601 gacccagcag gccagcgagg cgcaggcagc ggagtggctg aaagcccagc gcgagacaca
     6661 ggcagcatgg gccaagctgg ccaaggaatt gcgggaagtg gccagcgagg tgagcgacgc
     6721 cgcgcagagc gcccggagcg cggccagagg gtggcactgg aagctgtggc taaccgtgat
     6781 ggcggcttcc atgatgccta cgctggtgct gctgatcgca tcgttgctct tgctcgacct
     6841 gacgccactg acaacagagg acggctcgat ctggctgcgc ttggtggccc gatgaaaaac
     6901 gacagaacct tgcaggccat aggccgacag ctcaaggcca tgggctgtga gcgcttcgat
     6961 atcggcgtca gggatgccac caccggccag atgatgaacc gggaatggtc agcctccgaa
     7021 gtgctccaga acacgccatg gctcaagcgg atgaatgccc agggcaatga cgtgtatatc
     7081 aggcccgccg agcaggaacg ccatggtctg gtgctggtgg acgacctcag cgagctcgac
     7141 ctggaggaca tgaaagccga gggccgggaa ccggcgctga tcgtggagac cagcccgaag
     7201 aactatcagg catgggtcaa ggtggccgac gccgcaggcg gtgaactccg ggggcagatt
     7261 gcccggacgc tggccagcga gtacgacgcc gacccggcca gcgccgacag ccgccactat
     7321 ggccgcttgg cgggtttcac caaccgcaag gacaagcaca ccacccgcac cggctatcag
     7381 ccgtgggtgc tgctgcgtga gtccaagggc aagaccgcca ccgctggccc ggcgctggtg
     7441 cagcaggctg gccagcagat cgagcaggcc cagcggcagc aggagaaggc ccgcaggctg
     7501 gccagcctcg aactgcccga gcggcagctt agccgccacc ggcgcacggc gctggacgag
     7561 taccgcagcg agatggccgg actggtcaag cgctacggtg acgacctcag caagtgcgac
     7621 tttatcgccg cgcagaaact ggccagccgg ggccgcagtg ccgaggaaat cggcaaggcc
     7681 atggccgagg ccagcccggc gctggcagag cgcaagcccg gccacgaagc ggattacatc
     7741 gagcgcaccg tcagcaaggt tatgggtctg cccagcgtcc agcttgcgcg ggccgagctg
     7801 gcacaggcac cggcaccccg ccagcgaggc atggacaggg gcgggccaga tttcagcatg
     7861 taacgcttgc attagcgcta acgggtagta tactgttagc tctaatggag gatttgagat
     7921 gaaagaccat cgggacacgc agaccggcaa cctgttggcc acgcccaacg cgatcaggca
     7981 ggctaggttt gccgacgctc agcgaaaact gggccgcaag gcccgtaaga tatgggccac
     8041 agacgacgag gccgaggcgc tgcgtctgta cctggaagaa ctcagagcgg cgcagggcgg
     8101 gggtagtgac cccgccagcg cctaaccacc aactgcctga acaggaggca atc
//
