LOCUS       NZ_CP075730             1749 bp    DNA     circular CON 16-AUG-2023
DEFINITION  Escherichia coli strain CPE35 plasmid pE35-ColpVC, complete
            sequence.
ACCESSION   NZ_CP075730
VERSION     NZ_CP075730.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19296500
            Assembly: GCF_024499175.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Khine,N.O.
  TITLE     Colistin resistant E. coli isolates in pigs and pig farm environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Khine,N.O.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2021) Veterinary Microbiology, Chulalongkorn
            University, 39, Henry Dunant Road, Faculty of Veterinary Science,
            Bangkok, Bangkok 10400, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. galaxy verion 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/16/2023 04:23:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,854
            CDSs (total)                      :: 4,730
            Genes (coding)                    :: 4,508
            CDSs (with protein)               :: 4,508
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 59 of 222
            Pseudo Genes (incomplete)         :: 157 of 222
            Pseudo Genes (internal stop)      :: 40 of 222
            Pseudo Genes (multiple problems)  :: 35 of 222
            Pseudo Genes (short protein)      :: 4 of 222
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075730.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CPE35"
                     /isolation_source="Pig feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="pE35-ColpVC"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2017-06-10"
                     /note="mcr-3"
     gene            join(1359..1749,1..599)
                     /locus_tag="KKZ10_RS24165"
                     /old_locus_tag="KKZ10_24010"
     CDS             join(1359..1749,1..599)
                     /locus_tag="KKZ10_RS24165"
                     /old_locus_tag="KKZ10_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_039022372.1"
                     /translation="MKNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDSRWMFLTLTVRNCAIGELGDTLSRMNTAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVKHARWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKIEVKKYRRSPTKDKTESE"
                     /name="protein rep"
     Typing          complement(328..517)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.859"
                     /coverage="99.0"
                     /evalue="3.7200000000000003e-85"
ORIGIN
        1 tgagccggat gaatacggct ttccagcggc tgaaggatcg caaagagttc agaccagttc
       61 agggatggat ccgcaccacg gaagtcaccc gcggcagtga cggcagcgca cacccgcact
      121 tccacacact gatgatggtt ccgcctggca tgcttaacgg gaagagttac gtcaaacatg
      181 cgcgctgggt tgagctgtgg cgggagtgcc ttcgggtcag ctacgatccg aatgtcgatg
      241 tccgggcagt gaaaccccgg aagccgaagg acggcgaatc gctggcctgc gcgaccgccg
      301 aactggtgcg cggcgcggtg gccgagaccc tgaaatattc cacgaagccg gccgacatgg
      361 tcgccgatcc tgaatggttc ctcgaactga cccgccagac gcacaagcgc cgctttgtgg
      421 cgaccggcgg cgcactcaaa gacgtattga aactcgatca ggaaaccgat gccgacatgg
      481 tgatcgggga tgatatttcc gagggcgatg atgacggttc ccgcatcgcg ttcgagtgga
      541 aaatcgaggt caaaaaatac cggcgttcgc cgacgaaaga taaaaccgaa tcagagtaaa
      601 aacactgcga tgcaggatag ggcaaacgcc gcaaaatgac gtcatccgac atcattccgc
      661 cgcgttcgcc cagtgacctg cagtcggctc tggggtgtcg gggctgcgcc ctgacctggg
      721 gttttagatt gcggccatcg tgatggcggt aatacaaaac gcacgggaca agatgtgttt
      781 ttggagtacc gccgacacgc gacggacagt ccaaaatatc ttggtcaggg ctgcgccccg
      841 acaccctgcc aacacctcac gccgccagat taaaatctgc cagcgttcgc cgttcctgaa
      901 atcgtcaaac gctaaatcgt ggggtcccgc tggcttgcca gtgggttccg atttagtgat
      961 tgacgagcgc acccgtaaaa cactgccgac aggtggttgt gatcacgcag tgagcgcaac
     1021 cacctgataa gcgtttttgg ggtttgtggg gtccgattta acgtggattt tgggggcaaa
     1081 aaaatcgcgc gcgttccttg tatttatact taagggataa ctctggcgtt aagaataagc
     1141 ctcagccctt gtgccgcaag ggtttcagcg tgatttgcct gaaaatgact ttgaagttat
     1201 tctgtcggga gtggtcgcgt taaaagcgac ccccgcaacg acggccatcg ttcgggggcg
     1261 tgaccacctg taggaattct attttgcgac agacaacaca ggtgatacgg caatgataac
     1321 acgtcaagtg agccatcgcc cagtatgggg cggtgcgcat gaaaaatcct gaactccaga
     1381 acctcacgga ctattccccc tcagatgcgc cctgggacgc tcacagaagc gtttctgacg
     1441 acgtgggagg gatttacctg cttgcggccg aatatgagcg ctacggggcg cgtatggcct
     1501 cctgcggcgg tttactgcgt ttcgggtggt ctaccctcaa agaaaccgga gaaacgcgcc
     1561 tgaggctgcg tgaggcgcat ttctgccgtg tccggcactg tcctgtctgc cagtggaggc
     1621 gctccctgat gtggcaagcc cgtttctacc agtccctgcc gcgtatcgtg gcggattacc
     1681 ccgattcccg ctggatgttc ctgaccctga ccgtgcgcaa ctgtgcgatc ggggagctgg
     1741 gcgatacgc
//
