LOCUS       NZ_CP075726             9537 bp    DNA     circular CON 16-AUG-2023
DEFINITION  Escherichia coli strain CPE35 plasmid pE35-COLE10, complete
            sequence.
ACCESSION   NZ_CP075726
VERSION     NZ_CP075726.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19296500
            Assembly: GCF_024499175.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Khine,N.O.
  TITLE     Colistin resistant E. coli isolates in pigs and pig farm environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9537)
  AUTHORS   Khine,N.O.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2021) Veterinary Microbiology, Chulalongkorn
            University, 39, Henry Dunant Road, Faculty of Veterinary Science,
            Bangkok, Bangkok 10400, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. galaxy verion 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/16/2023 04:23:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,854
            CDSs (total)                      :: 4,730
            Genes (coding)                    :: 4,508
            CDSs (with protein)               :: 4,508
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 59 of 222
            Pseudo Genes (incomplete)         :: 157 of 222
            Pseudo Genes (internal stop)      :: 40 of 222
            Pseudo Genes (multiple problems)  :: 35 of 222
            Pseudo Genes (short protein)      :: 4 of 222
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075726.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CPE35"
                     /isolation_source="Pig feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="pE35-COLE10"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2017-06-10"
                     /note="mcr-3"
     gene            1..951
                     /locus_tag="KKZ10_RS24060"
                     /old_locus_tag="KKZ10_23915"
     CDS             1..951
                     /locus_tag="KKZ10_RS24060"
                     /old_locus_tag="KKZ10_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001293213.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     gene            959..1372
                     /locus_tag="KKZ10_RS24065"
                     /old_locus_tag="KKZ10_23920"
     CDS             959..1372
                     /locus_tag="KKZ10_RS24065"
                     /old_locus_tag="KKZ10_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001229901.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTSE
                     LLRVFGAFKAVHHEQIDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
                     /name="hypothetical protein"
     gene            complement(1608..6488)
                     /locus_tag="KKZ10_RS24070"
                     /old_locus_tag="KKZ10_23925"
     CDS             complement(1608..6488)
                     /locus_tag="KKZ10_RS24070"
                     /old_locus_tag="KKZ10_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005204747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="WP_256634011.1"
                     /translation="MNALSALLTKIEQASPTQRDKGTTFENLCVQYFLHEPKYAELYSD
                     VLSYGGWVSQYGETVGITKKKDDGIDLVAVTKTGEFHAIQCKNYNQTKIAKKDIDSFLA
                     ASDKTYFTLRYIVASTDNWTEEAKNMLRDKAVPVTALSLTDLEQSALDWSQFDFDPAYK
                     PVMKAKKQLRPHQTPALEAVKRGLATADRGKLIMACGTGKTFTSLRIAEAVAGRGKTVL
                     FLVPSLALLSQTLDEWTQDTLIDLRCFAVCSDSDVGKKNHDDNVVVGISDLKYPATTNA
                     NSLVKAFNQPDIFGSDKPPYMNVVFSTYHSVEVIHQAQKLGFPAFDFIICDEAHRTTGA
                     TFEGDDESAFVRIHDNAYIAGQKRLYMTATPRIFGDDAKETEGVTLCSMDDKSLYGDDL
                     YVITFSKAVQLGILCDYKVIVLAVEEKHVSRRIQSLLKDENNQLKVDDAAKIVGCWKAL
                     AKQGLAGPDGVHPNAMKRAVAFCQVIEKDFRGKTHKVSSKLITEMFGAVVEAYQASEIE
                     LLRETAPDKAIDPSLKLQCQVKHVDGSMNATEKKSKIEWLKADTDENVCRILSNVRCLS
                     EGVDVPSLDAVLFLTPRSSQVDVVQSVGRVMRRAEGKELGYVILPVVIPAGEEPENALN
                     NNQAYRVVWQVLNALRAHDDRFDAMINKLEFNGKDTSRMEVIAVADKVVKKTKRQTKKS
                     ELAGKARKSSGIGSAVSPVPEQFDIEFSVGEIERALYAKIVKKCGNRHHWEDWANDIAK
                     IARTHIDRIHGILENPGNQVEISAFRAFADELRDDLNNSVSDDEIIEMLAQHLITKPVF
                     DALFADYNFTDHNPMSMAMQNVLNTLQEHHLEKEASTLNSFYESVKMRADGITTAEGKQ
                     QIIVQLYDKFFRNAFPRMTERLGIVYTPVEVVDFIIHSVNDVLKKEFGQTLADKGVHIL
                     DPFTGTGTFITRLLQSGLIPSDKLAYKFKNEIHANEIVLLAYYIAAINIEAVYHSITEE
                     SEYTPFEGICLTDTFEMYEKDDLVSEVLVDNSERRKRQKALDIRVIIGNPPYSAGQRSE
                     NDNNQNISYPHLDKRISDTYAAYSSATLQKNLYDSYIRAIRWASDRIGEQGVIGFVTNG
                     GYIDSNSADGLRKCLAEEFSSLYFFHLRGNARTSGEVRRKEKDNVFGQGTRTPIVIAIL
                     VKNPVATQTGQIYFHDIGDYLTKEEKLETISDFGSIDGIEKTHGWQKITPDVYNDWLNQ
                     RDDSFYDFIAIGNKKDEKEKSFFHNYSNGVVTARDSWVYQYSNKKLRVNISDMINFYNN
                     ELSTLSRVGDLNGKDVGDLIDNNPTKISWTRALKNDLKKGRNLEFDDLSIYVSMYRPFI
                     KCWMYFNRRLNEMVYQMPQIFPFPNSENVLITLNVNPKKGFCVLLTDVVPDFHLLGDVQ
                     CFPLYVYESSDTGVVRKNAINNDVLIEINSHYSLNNITNEDVFFYIYGLLHSNDYRATY
                     KDNISKELPRIPLVKNIDDFFVYSNSGRELAELHLKYEKVEPYKADIDTGSLSYSQLGK
                     DDFYVEKMKFAKKGEKDTVIYNNKIRIKNIPTEAYDYVVNGKSALEWVMERQGVSTHKD
                     SGIVNDANDWAIETMHNPRYPLELFLRVITVSLETQKIVNSLPKLDI"
                     /name="type ISP restriction/modification enzyme"
     gene            complement(6601..7185)
                     /locus_tag="KKZ10_RS24075"
                     /old_locus_tag="KKZ10_23930"
     CDS             complement(6601..7185)
                     /locus_tag="KKZ10_RS24075"
                     /old_locus_tag="KKZ10_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_010891053.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQAASSQLQQQSETLSR
                     VIRELDSASGNMTDTVRKSVSSALTQVEKELKQAGLAQQKPATEALNQAADTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVLASCVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRTTYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(7198..8313)
                     /gene="mobA"
                     /locus_tag="KKZ10_RS24080"
                     /old_locus_tag="KKZ10_23935"
     CDS             complement(7198..8313)
                     /gene="mobA"
                     /locus_tag="KKZ10_RS24080"
                     /old_locus_tag="KKZ10_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_016946648.1"
                     /translation="MASYHLSVKTGGKGSASPHADYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDPEQYFKRANTKEPERGGAKKARFG
                     ETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQR
                     FDTEQLQAILERREAERQVQQSRDERDSVIDVTTSLREALSERDTLTLKQELKSEPEQE
                     SHSGRTFDFEKEPDKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERQRERER
                     LAEKQRQQEKERQRLAEQIRQKPDKGWSFSR"
                     /name="mobA"
     gene            8477..8944
                     /gene="traD"
                     /locus_tag="KKZ10_RS24085"
                     /old_locus_tag="KKZ10_23940"
     CDS             8477..8944
                     /gene="traD"
                     /locus_tag="KKZ10_RS24085"
                     /old_locus_tag="KKZ10_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_016946647.1"
                     /translation="MNKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     Typing          776..951
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(7202..8313)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.07"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtctgaat tagttgtttt caaagcaaat gaactagcga ttagtcgcta tgacttaacg
       61 gagcatgaaa ccaagctaat tttatgctgt gtggcactac tcaaccccac gattgaaaac
      121 cctacaagga aagaacggac ggtatcgttc acttataacc aatacgctca gatgatgaac
      181 atcagtaggg aaaatgctta tggtgtatta gctaaagcaa ccagagagct gatgacgaga
      241 actgtggaaa tcaggaatcc tttggttaaa ggctttgaga ttttccagtg gacaaactat
      301 gccaagttct caagcgaaaa attagaatta gtttttagtg aagagatatt gccttatctt
      361 ttccagttaa aaaaattcat aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa
      421 tactctatga ggatttatga gtggttatta aaagaactaa cacaaaagaa aactcacaag
      481 gcaaatatag agattagcct tgatgaattt aagttcatgt taatgcttga aaataactac
      541 catgagttta aaaggcttaa ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact
      601 tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac gttgattttc
      661 caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa ccagataaaa
      721 atgaatggtg acaaaatacc aacaaccatt acatcagatt cctacctaca taacggacta
      781 agaaaaacac tacacgatgc tttaactgca aaaattcagc tcaccagttt tgaggcaaaa
      841 tttttgagtg acatgcaaag taagtatgat ctcaatggtt cgttctcatg gctcacgcaa
      901 aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg aggttcttat
      961 ggctcttgta tctatcagtg aagcatcaag actaacaaac aaaagtagaa caactgttca
     1021 ccgttacata tcaaagggaa aactgtccat atgcacagat gaaaacggtg taaaaaagat
     1081 agatacatca gagcttttac gagtttttgg tgcattcaaa gctgttcacc atgaacagat
     1141 cgacaatgta acagatgaac agcatgtaac acctaataga acaggtgaaa ccagtaaaac
     1201 aaagcaacta gaacatgaaa ttgaacacct gagacaactt gttacagctc aacagtcaca
     1261 catagacagc ctgaaacagg cgatgctgct tatcgaatca aagctgccga caacacggga
     1321 gccagtgacg cctcccgtgg ggaaaaaatc atggcaattc tggaagaaat agcgctttca
     1381 gccggcaaac cggctgaagc cggatctgcg attctgataa caaactagca acaccagaac
     1441 agcccgtttg cgggcagcaa aacccgtact tttggacgtt ccggcggttt tttgtggcga
     1501 gtggtgttcg ggcggtgcgc gcaagatcca ttatgttaaa aacgacagga ttcatgaaaa
     1561 tcatcagaat tgtgcaggat ggtttttcca tcctgcacac ctattgatta gatatctagt
     1621 ttaggcaggc tattaacaat tttctgagtc tcaagactta cagtgatcac tcgcaggaac
     1681 aactctaacg gatatcgtgg gttatgcatg gtttcaattg cccagtcatt agcatcattc
     1741 acaataccgc tatctttatg ggtagaaacg ccttgtcgtt ccatcaccca ttcaagagca
     1801 gacttgccat tcacaacgta gtcataggct tcagttggaa tgttttttat gcgaatttta
     1861 ttgttataga tgaccgtatc tttttcacct tttttggcga acttcatctt ctcgacataa
     1921 aaatcgtctt tgcctaactg agaatagctc agggatcccg tatcaatatc agccttgtat
     1981 ggctcaactt tttcatattt taaatgcaat tcagcaagtt cacgaccact attagaataa
     2041 acaaaaaaat catcaatatt tttaactaaa ggaatacgag gtaactcttt actaatatta
     2101 tccttatatg tagccctgta gtcattggaa tgaagcaatc catatatata gaaaaagaca
     2161 tcctcattag taatattatt taacgaataa tgagaattta tctcaatcaa cacatcatta
     2221 ttaatggcat tttttcttac gacaccagta tccgaggatt catacacata taaagggaag
     2281 cactgaacat cacctaacaa gtggaagtca ggtacgacgt ctgtcaatag tacgcaaaat
     2341 cctttttttg gatttacatt taatgttatc agcacatttt cagagtttgg gaatggaaat
     2401 atctgtggca tttgataaac catttcattc agtcttctat taaaatacat ccagcatttt
     2461 ataaatggtc tatacatcga cacatatata gataagtcat caaattccaa attacgacct
     2521 ttttttaaat cgttctttag ggctctagtc caacttattt ttgttgggtt attatcaata
     2581 aggtcgccta catccttacc atttaaatca cctactctag aaagagtaga caattcgtta
     2641 ttgtaaaaat taatcatatc tgaaatgtta actcttaact ttttattaga gtattgataa
     2701 acccaagagt cccttgctgt aacgacccca tttgaatagt tatgaaaaaa gcttttttct
     2761 ttttcatcct ttttattacc tatcgcaatg aagtcataaa aactgtcatc tcgctgattc
     2821 agccagtcgt tgtagacatc tggtgtgatt ttctgccagc catgcgtttt ctcaataccg
     2881 tcaatactgc caaaatcgga aattgtctcc agcttttctt cttttgtcag gtaatcacca
     2941 atatcatgaa aataaatctg acccgtctgt gttgctaccg gattttttac cagaatagca
     3001 attacaatcg gcgttcttgt tccctgacca aaaacgttat ctttttcttt gcggcgaacc
     3061 tctcctgacg ttctagcatt ccctctcaga tggaaaaaat acaggctact gaactcttcc
     3121 gccagacact tacgaagtcc atcagccgag ttagagtcga tatagccacc attagtgaca
     3181 aaaccgatca ccccctgctc tccgatacgg tcacttgccc aacggatagc gcgaatataa
     3241 gaatcatata agtttttttg cagcgttgct gatgaatacg ctgcatacgt atcggatatc
     3301 ctcttatcca gatgagggta gctaatattc tgattattat cattctctga tctctgaccc
     3361 gctgaataag gtggatttcc gataatcacc cgaatatcca gtgccttctg ccgcttacgg
     3421 cgttcactgt tatcaaccaa aacctcactg accaggtcat ctttctcata catttcaaag
     3481 gtgtcagtca gacaaatccc ttcaaatggc gtgtactctg attcttccgt gatgctgtga
     3541 tacaccgcct caatattaat ggcggcaatg taataggcta acaagacgat ttcgttggca
     3601 tggatttcat ttttaaactt gtaagccagt ttatctgacg ggataagacc actttgcagc
     3661 aggcgggtga taaacgtacc ggttccggtg aatgggtcaa gaatatgcac acctttgtcg
     3721 gcgagtgttt gtccgaactc ctttttcagc acgtcattca cgctgtgaat aataaaatca
     3781 accacctcaa caggcgtata cacaattccc agacgctcag tcattctcgg gaacgcgttt
     3841 ctgaaaaact tatcgtacaa ctgtacgata atctgctgtt taccttctgc ggtggtaatg
     3901 ccgtctgcac gcatttttac cgactcatag aagctgttca gcgtgctggc ttctttttcg
     3961 agatgatgct cctgtaacgt gttaagcacg ttctgcatcg ccatactcat cgggttatgg
     4021 tcggtaaaat tgtaatcggc aaacagggca tcaaagaccg gcttggttat cagatgctgt
     4081 gccagcattt caatgatttc atcgtcggaa acactgttat tcagatcatc acgcagttca
     4141 tcggcaaatg ccctgaaggc actgatttct acttgattac ccggattttc gagaatgccg
     4201 tgaatgcggt cgatatgcgt gcgggcaatt ttagcgatat cgttcgccca gtcttcccag
     4261 tgatgccggt taccgcattt tttaacgatt tttgcgtaca gggcacgttc aatctcacca
     4321 acggaaaact caatatcaaa ctgctcagga acaggactca cggctgaacc aatgccactg
     4381 cttttacgcg cttttcctgc cagttcagat tttttcgtct gccgtttagt tttcttcacc
     4441 actttatcgg ctaccgcgat cacttccatt ctgctggtat ctttaccgtt aaactccagc
     4501 ttgttgatca tggcatcaaa gcggtcatca tgcgccctga gtgcattcag cacctgccag
     4561 acaacacggt aggcctgatt attgttcaac gcattctccg gctcttctcc ggcgggaatg
     4621 acaaccggca gaatgacata gcccagctct ttaccttcgg cacgccgcat aacacgcccg
     4681 acagactgca ccacatccac ctgagagctt ctcggcgtta aaaacaacac tgcatccaga
     4741 gaaggcacat ccacaccttc agataaacaa cggacgttag acaatatccg gcagacattt
     4801 tcatccgtat ccgctttcag ccattcaatt ttggattttt tctcagtggc attcatgctg
     4861 ccgtcaacgt gcttcacctg acactgaagt tttaatgacg gatcaatagc cttatccggt
     4921 gcggtttccc tgagaagttc aatctcggat gcctgatagg cttcaaccac tgctccgaac
     4981 atttcagtga tcagtttaga actgacctta tgcgttttgc cgcgaaagtc tttttctatc
     5041 acctgacaga acgccactgc ccgcttcatc gcattaggat gaacgccatc aggccccgcc
     5101 agcccctgct ttgccagtgc tttccagcaa cccacaattt ttgccgcatc atcgactttg
     5161 agctgattat tttcatcctt cagcagagac tgaatgcggc ggctgacatg cttttcttcc
     5221 acggcaagca caataacttt gtaatcacac aaaatgccca actgaacggc tttagagaag
     5281 gtaatgacat acaggtcatc accgtaaagg cttttgtcat ccattgagca taaggtgacc
     5341 ccttcagtct ctttcgcatc gtccccgaaa atgcgcggag tcgcggtcat gtacaggcgt
     5401 ttttgaccgg cgatataggc gttgtcgtga atacgaacaa aggcggactc atcatcccct
     5461 tcaaaggtcg cacctgtggt ccggtgagct tcatcacaaa tgatgaaatc aaatgcagga
     5521 aaacccagtt tttgcgcctg atggatgacc tcaacagagt ggtaggtaga aaacaccacg
     5581 ttcatataag gcggcttatc actgccgaag atatcaggct gattaaacgc tttgacgagg
     5641 ctgttcgcat tcgtggtcgc cggatatttc aggtcgctga taccaacaac gacattgtca
     5701 tcatggtttt tcttacccac atcactgtca gagcagacag caaaacagcg caggtctatc
     5761 agcgtatcct gtgtccattc atccagcgtc tgactcaaca atgccagact gggcacaagg
     5821 aacagtacgg ttttcccccg tccggcaacc gcttcagcga tacgcagcga ggtgaaggtt
     5881 ttccctgtgc cgcacgccat aataagcttg ccgcggtcgg cagtcgccag ccctcttttc
     5941 acggcttcaa gcgcgggagt ctgatgaggt cggagttgtt tttttgcttt catcaccggt
     6001 ttgtaggccg gatcaaaatc gaattgtgac cagtccagtg cgctttgctc aaggtctgtc
     6061 aggcttaacg cagtgacagg aacggccttg tcccgcagca tatttttagc ttcctccgtc
     6121 cagttatccg tagaggcaac gatatagcgg agcgtaaaat acgttttatc ggaggcagcc
     6181 agaaaactat caatatcctt tttggcgatt ttagtctgat tgtagttttt gcactggata
     6241 gcatgaaact cccccgtttt ggttacggcg accaaatcaa tgccatcatc ttttttcttc
     6301 gtgatcccaa cggtttcacc gtactgagaa acccatccgc catagctcag cacgtcactg
     6361 tacagctcgg catatttagg ttcatggagg aaatactgca cacaaaggtt ttcaaaggtt
     6421 gtacctttgt cccgctgtgt tggtgaagcc tgttcaattt ttgtcagcaa tgcagaaagt
     6481 gcattcatcg ttgatatcct taatgtcctt tatttgctgg ggttatcaga tccccccgac
     6541 acgtttaatt aatgctttct ccgccggaga tcgacgcaca gcgttctgtg ctctatgatg
     6601 ttatttctta ataatcatcc aggtattctc tttatcacca tacgtagtgc gagtgtccac
     6661 cttaacgcag ggctttccgt cacagcgcga tatgtcagcc agcggggctt tcttttgcca
     6721 gaccgcttcc atcctctgca tttcagcaat ctggctatac ccgtcattca taaaccacgt
     6781 aaatgccgtc acgcaggaag ccaggacgaa gaatatcgtc agtacaagat aaatcgcgga
     6841 tttccacgta tagcgtgaca tctcacgacg catttcatgg atcatcgctt tcgccgtatc
     6901 ggcagcctga ttcagcgctt ctgtcgccgg tttctgctgt gctaatccgg cttgtttcag
     6961 ttctttctca acctgagtga gcgcggaact caccgatttc ctgacggtgt cagtcatatt
     7021 accggacgcg ctgtccagct cacgaatgac cctgctcagc gtttcacttt gctgctgtaa
     7081 ttgtgatgag gcggcctgaa actgttctgt cagagaagta acacgctttt ccagcgcctg
     7141 atgatgcccg ataagggcgg caatttgttt aatttcgtcg ctcatacaaa atcctgccta
     7201 tcgtgagaat gaccagcctt tatccggctt ctgtcgtatc tgttcggcga gtcgctgtcg
     7261 ttctttctcc tgctgacgct gtttttccgc cagacgttcg cgctctctct gcctttccat
     7321 ctcctgatgt atcccctgga actccgccat cgcatcgtta acaagggact gaagatcgat
     7381 ttcttcctgt atatccttca tggcatcact gaccagtgcg ttcagcttgt caggctcttt
     7441 ttcaaaatca aacgttctgc cggaatggga ttcctgctca ggctctgact tcagctcctg
     7501 ttttagcgtc agagtatccc tctcgctgag ggcttcccgt aacgaggtag tcacgtcaat
     7561 tacgctgtca cgttcatcac gggactgctg cacctgcctt tcagcctccc tgcgctcaag
     7621 aatggcctgt agctgctcag tatcgaatcg ctgaacctga cccgcgccca gatgccgctc
     7681 aggctcacgg tcaatgccct gcgccttcag ggaacgggaa tcaacccggt cagcgtgctg
     7741 ataccgttca aggtgcttat tctggaggtc agcccagcgt tccctctggg caacaaggta
     7801 ttctttgcgt tcggtcggtg tttccccgaa acgtgccttt tttgcgccac cgcgctccgg
     7861 ctctttggtg ttagcccgtt taaaatactg ctcagggtca cggtgaatac cgtcattaat
     7921 gcgttcagag aacatgatat gggcgtgggg ctgctcgcca ccggctatcg ctgctttcgg
     7981 attatggata gcgaactgat aggcatggcg gtcgccaatt tcctgttgga caaaatcgcg
     8041 gacaagctca agacgttgtt cgggttttaa ctcacgcggc agggcaatct cgatttcacg
     8101 gtaggtacag ccgttggcac gttcagacgt gtcagcggct ttccagaact cggacggttt
     8161 atgcgctgcc cacgccggca tattgccgga ctccttgtgc tcaaggtcgg agtctttttc
     8221 acgggcatac tttccctcac gcgcaatata atcggcatga ggagaggcac tgccttttcc
     8281 gccggttttt acgctgagat gataggatgc catcgtgttt tatcccgctg aaggcgcgca
     8341 ccgtttctga acgaagtgaa gaaacgtcta agtgcgccct gataaataaa agagttatca
     8401 gggattgtag tgggatttga cctcctctgc catcactgag cataatcatt ccgttagcat
     8461 tcaggaggta aacagcatga ataaaagcga aaaacaggaa caatgggcag cagaaagagt
     8521 gcagtatatt cgcggcttaa agtcgccgaa tgagcaacag aaacttatgc tgatactgac
     8581 ggataaagca gataaaacag cacaggatat caaaacgctg tccctgctga tgaaggctga
     8641 acaggcagca gagaaagcgc aggaagccag agcgaaagtc atgaacctga tacaggcaga
     8701 aaagcgagcc gaagccagag ccgcccgtaa agcccgtgac catgccctgt accagtctgc
     8761 cggattgctt atcctggcgg gtctggttga cagtaagacg ggtaagcctg ttgatgatac
     8821 cgctgcctta ctgggtgcat tagccagtct gaatgacctg tcacgggata atccgaagtg
     8881 gtcagactgg aaaatcagag ggcaggaact gctgaacagc aaaaagtcag atagcaccac
     8941 atagcagacc cgccataaaa cgccctgaga agcccgtgac gggcttttct tgtattatgg
     9001 gtagtttcct tgcatgaatc cataaaaggc gcctgtagtg ccatttaccc ccattcactg
     9061 ccagagccgt gagcgcagcg aactgaatgt cacgaaaaag acagcaactc aggtgcctga
     9121 tggtcggaga caaaaggaat attcagcgat ttgcccgagc ttgcgagggt gctacttaag
     9181 cctttagggt tttaaggtct gttttgtaga ggagcaaaca gcgtttgcga catccttttg
     9241 taatactgcg gaactgacta aagtagtgag ttatacacag ggctgggatc tattcttttt
     9301 atcttttttt attctttctt tattctataa attataacca cttgaatata aacaaaaaaa
     9361 acacacaaag gtctagcgga atttacagag ggtctagcag aatttacaag ttttccagca
     9421 aaggtctagc agaatttaca gatacccaca actcaaagga aaaggactag taattatcat
     9481 tgactagccc atctcaattg gtatagtgat taaaatcacc tagaccaatt gagatgt
//
